CN102302801A - Silk-fibroin-coated polypropylene mesh and preparation method thereof - Google Patents
Silk-fibroin-coated polypropylene mesh and preparation method thereof Download PDFInfo
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- CN102302801A CN102302801A CN201110271009A CN201110271009A CN102302801A CN 102302801 A CN102302801 A CN 102302801A CN 201110271009 A CN201110271009 A CN 201110271009A CN 201110271009 A CN201110271009 A CN 201110271009A CN 102302801 A CN102302801 A CN 102302801A
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Abstract
The invention relates to a silk-fibroin-coated polypropylene mesh as well as a preparation method and application thereof. The silk-fibroin-coated polypropylene mesh is prepared by coating the surface of a polypropylene mesh used as a support with a silk fibroin coating liquid to form a layer of silk fibroin coating; and the pores of the prepared polypropylene mesh are open. The modification process of the silk-fibroin-coated polypropylene mesh is simple and feasible, and does not use any toxic and harmful reagents; on the one hand, the tensile property of the repaired part is enhanced by utilizing the characteristics that the polypropylene mesh has high porosity and surrounding tissues can well grow; and on the other hand, the silk-fibroin-coated polypropylene mesh can be applied in clinical female pelvic floor reconstruction surgery by utilizing the characteristics that the regenerated silk fibroin has biocompatibility and can promote the growth of mechanocytes, thereby accelerating tissue growth and reducing postoperative complications.
Description
Technical field
The present invention relates to a kind of organizational project and medical science articles for use field, relate in particular to a kind of fibroin coating polypropylene mesh and its production and application.
Background technology
At present; For female pelvic organ prolapse (the pelvic floor prolapse that damages at the bottom of the basin and functional deterioration causes; POP) and stress incontinence (stress urinary incontinence; Be called for short: SUI) etc. treatment of diseases is general adopts traditional surgical intervention scheme, promptly carries out the vagina front and rear wall through operation and repairs and uterectomy.Yet above-mentioned two kinds of therapeutic schemes can only be repaired the female pelvic organ of prolapsus, and can't improve the intensity of tissue itself, can't reach at the bottom of the basin anatomical structure and rebuild, thereby thoroughly cure the purpose of diseases such as female pelvic organ prolapse, so the postoperative recurrence rate is high.In recent years, in order to overcome tradition for the deficiency in the female pelvic organ of surgical intervention reparation prolapsus, polypropylene mesh is that the polymer fabric sheet material of representative is widely used at the bottom of the basin in the reconstruction operations.These polymer fabric sheet material porositys are higher, and tissue merges preferable, has good biomechanical property, thereby can effectively improve the intensity of tissue itself.Adopt the postoperative recurrence rate of these synthetic net sheet material to be starkly lower than kposthesis at the bottom of traditional basin.
Yet; Adopt the polymer fabric sheet material can effectively treat diseases such as organ comes off at the bottom of the basin; But the biocompatibility of above-mentioned polymer fabric sheet is relatively poor; After implantation; Thereby may cause the erosion that influences quality of life, the especially polypropylene mesh of patient's postoperative such as a series of complication relevant such as corroding exposure, infection, pain, sexual intercourse discomfort with the net sheet, often relevant with the histocompatibility of implanting polypropylene mesh; And need exenterate net sheet once more, bring misery to the patient.
Implant the back at human body and be prone to produce problems such as complication in order to overcome above-mentioned polymer fabric sheet, thereby people begin to demand and carry out the material behavior that finishing improves the polymer fabric sheet at the polymer fabric sheet, improve its biocompatibility.Method to the transformation of net sheet material has at present: collagen covering, fatty acid coating, polyvinylidene fluoride coating and metallic element titanium coating etc.Wherein, what effect was best is collagenic coating, and it can improve histocompatibility, reduces inflammatory reaction and tissue fibering, promotes collagen formation etc.But because the separating and purifying technology of collagenic coating is complicated, the early-stage preparations time is longer, and its extensive use of factor restriction such as cost an arm and a leg.
Afterwards, find in to the different increment study of human body cell strain on silk protein membrane state, inside and outside scholar: fibroin is the same with collagen protein, can promote the growth of cell.
Fibroin is a kind of natural structure property albumen, and with respect to collagen protein, it has, and the source is wide, and price is low, and preparation is simple, and advantage such as it is better to possess biocompatibility equally, and complication rate is low.Fibroin albumen is made up of 18 seed amino acids, and wherein main have three kinds of simple aminoacid: glycine, and alanine and serine are formed, and they occupy 85% of Tot Prot approximately.A lot of hydrophilic amino acid residues are arranged in the fibroin, and it is increasingly extensive in the application of medical field that its excellent biological compatibility and nontoxic, nonirritant make it; Like medical fibroin protein skin regeneration film, cell culture medium, medicine control carrier; Medical dressing, tissue engineering bracket material etc.But up to now, still there is not the relevant report that fibroin coating polypropylene mesh is used both at home and abroad in reconstructive surgery at the bottom of the department of obstetrics and gynecology basin.
Summary of the invention
The invention provides a kind of fibroin coating polypropylene mesh with and preparation method thereof; The defective that it exists to embedded material used in the reconstructive surgery at the bottom of the above-mentioned existing department of obstetrics and gynecology basin; Surface-coated one deck fibroin coating at polypropylene mesh; Thereby increase the biocompatibility of polypropylene mesh, avoid the generation of the relevant complication of polymer fabric sheet.
The present invention's a kind of fibroin coating polypropylene mesh and preparation thereof and application realize its purpose through following technical scheme:
A kind of fibroin coating polypropylene mesh wherein, comprises the polypropylene mesh as support; On said polypropylene mesh surface, adopt fibroin coating liquid parcel one deck fibroin coating; And the hole of said polypropylene mesh is the unlatching shape.
Above-mentioned method, wherein, in the said fibroin coating liquid, the volumetric concentration of fibroin is 1 ~ 8%.Wherein, special preferred volume concentration is 4 ~ 6% fibroin.
The method for preparing of above-mentioned fibroin coating polypropylene mesh wherein, may further comprise the steps:
The preparation silk protein solution: and with polypropylene mesh in being soaked in said silk protein solution more than 6 hours, fully soak into, thereby on polypropylene mesh, apply one deck silk protein solution coating;
With the polypropylene mesh that scribbles the silk protein solution coating after soaking into take out, drying, and be positioned over and soak 20 ~ 40 minutes after drying in the ethanol, make fibroin coating polypropylene mesh.
Above-mentioned method, wherein, repeat polypropylene mesh be soaked in fully soak in the said silk protein solution with drying steps repeatedly after, more dried polypropylene mesh is soaked in the alcoholic solution.
The application of above-mentioned fibroin coating polypropylene mesh, wherein, the mass concentration of said alcoholic solution is 60 ~ 90%.
Above-mentioned method wherein, also comprises the preparation process of silk protein solution, the steps include: with Bombyx bombycis remove pupa successively, come unstuck handle after, dissolving; And the solution that obtains is carried out deionized water dialyse, obtain the regenerated silk protein solution; Dilute said regenerated silk protein solution, obtain volumetric concentration and be 1 ~ 8% fibroin coating liquid.
Above-mentioned method, wherein, Bombyx bombycis goes coming unstuck behind the pupa to comprise in handling, and earlier Bombyx bombycis is placed 1 ~ 5% sodium bicarbonate to handle under 90 ~ 100 ℃ of conditions 30 ~ 90 minutes, takes out, and cleans with deionized water; Behind repetition said process 1 ~ 3 time, drying is 30 ~ 90 minutes under 60 ~ 70 ℃, obtains boiled silk; Afterwards under 30 ~ 60 ℃ of conditions, boiled silk will be dissolved in the lithium-bromide solution of 7 ~ 10mol/L and handle 30 ~ 90 minutes.
Above-mentioned method, wherein, in said Bombyx bombycis deionized water dialysis procedure, will being dissolved in silkworm silk solution in the lithium bromide, to pour residual molecular weight into be in 10000 ~ 14000 the fiber bag filter, with deionized water dialysis 2 ~ 3 days, thereby to obtain fibroin coating liquid.
Above-mentioned method wherein, also comprises the cleaning course of putting into said silk protein solution polypropylene mesh before, and its step is after elder generation carries out ultrasonic cleaning, Cement Composite Treated by Plasma with polypropylene mesh, to obtain clean polypropylene mesh.
A kind of application of fibroin coating polypropylene mesh wherein, is used as the fibroin coating polypropylene mesh that makes the timbering material of female pelvic reconstructive surgery.
Adopt the advantage of the method for preparing of a kind of fibroin coating of the present invention polypropylene mesh to be:
1. the present invention is through the surface-coated fibroin coating at polypropylene mesh; Thereby obtain fibroin coating polypropylene mesh; It utilizes the characteristics that the polypropylene mesh porosity is high, the fine title deed for land of surrounding tissue ability is gone into growth on the one hand, strengthens the stretch-proof performance of repairing the position; Utilize proteic biocompatibility of regenerated silk and the effect that promotes fibroblastic growth on the other hand, healing acceleration reduces complication, and the present invention can be used for clinical female pelvic reconstructive surgery, effectively reduces the generation of Reconstruction infectious-related complication at the bottom of the basin.
2. the method for preparing process of fibroin coating polypropylene mesh is simple among the present invention, does not relate to any poisonous and harmful reagent, and the follow-up use of medical patch is had no adverse effect; And fibroin to have a source wide, price is low, preparation is simple; Thereby reduced medical cost greatly, practical.
Description of drawings
Fig. 1 is undressed polypropylene mesh stereoscan photograph;
Fig. 2 is the stereoscan photograph of fibroin coating polypropylene mesh;
Fig. 3 is for having fibroin coating polypropylene mesh group to implant to draw materials in 30 days Masson dyeing photo;
Fig. 4 implants the Masson dyeing photo of drawing materials in 30 days for no fibroin coating polypropylene mesh group;
Fig. 5 is for having fibroin coating polypropylene mesh group to implant to draw materials in 90 days Masson dyeing photo;
Fig. 6 implants the Masson dyeing photo of drawing materials in 90 days for no fibroin coating polypropylene mesh group;
Fig. 7 implants 90 days transmission electron microscope photos of drawing materials for fibroin coating polypropylene mesh group;
Fig. 8 implants 90 days transmission electron microscope photos of drawing materials for no fibroin coating polypropylene mesh group;
Fig. 9 implants 30, the 60 and 90 days pull test curve charts of drawing materials in back for fibroin coating polypropylene mesh group;
Figure 10 implants 30, the 60 and 90 days pull test curve charts of drawing materials in back for no fibroin coating polypropylene mesh group.
The specific embodiment
The invention provides a kind of fibroin coating polypropylene mesh and its production and application.Fibroin coating polypropylene mesh among the present invention is by forming as the polypropylene mesh of support and the fibroin coating that adopts fibroin coating liquid to be coated in said polypropylene mesh surface.Wherein, after the said fibroin coating of said polypropylene mesh surface-coated, the hole of said polypropylene mesh does not seal, and still is the unlatching shape.
Wherein, in the said fibroin coating liquid, the volumetric concentration of fibroin is 1 ~ 8%, preferred 4 ~ 6%.
The method for preparing of said fibroin coating polypropylene mesh may further comprise the steps:
Step 1, fibroin coating liquid preparation: with Bombyx bombycis remove pupa successively, come unstuck handle after, dissolving; And the solution that obtains is carried out deionized water dialyse, obtain the regenerated silk protein solution; Dilute said regenerated silk protein solution, obtain volumetric concentration and be 1 ~ 8% fibroin coating liquid;
Step 2, fibroin coating polypropylene mesh preparation: place the abundant infiltration of described fibroin coating liquid after 12 ~ 24 hours the polypropylene mesh of cleaning, take out dry; Repeat above-mentioned polypropylene mesh and in fibroin coating liquid, soak into, drying steps 4 ~ 6 times; Be positioned over afterwards and handle 20 ~ 40 minutes in the alcoholic solution, after drying makes fibroin coating polypropylene mesh.
The present invention has also comprised the application of said fibroin polypropylene mesh; Described fibroin polypropylene mesh is suitable as timbering material and is applied in the female pelvic reconstructive surgery; Said fibroin polypropylene mesh; Can strengthen the stretch-proof performance of repairing the position; But also have excellent biological compatibility and the effect that promotes fibroblastic growth, help the reparation and the growth of tissue.
Set forth the present invention and advantage thereof in detail through specific embodiment below, but concrete protection domain of the present invention never is confined to following embodiment.
Embodiment 1
Step 1: common polypropylene mesh is carried out ultrasonic cleaning and Cement Composite Treated by Plasma, the polypropylene mesh that preparation is clean.
Step 2: the pure silk protein solution that purchase is obtained adopts deionized water to be diluted to concentration volumetric concentration 4 ~ 6%, obtains the regenerated silk protein solution.
Step 3: the polypropylene mesh of the cleaning that obtains in the step 1 inserted in the regenerated silk protein solution that obtains in the step 2 fully soak into, carry out the regenerated silk protein solution on the surface of polypropylene mesh and apply; Take out dry after 15 hours; After repeating the regenerated silk protein solution coating procedure five times of above-mentioned polypropylene mesh, place in 70% the ethanol again and handled 20 ~ 40 minutes, dry back obtains fibroin coating polypropylene mesh.
Embodiment 2:
Step 1: common polypropylene mesh is carried out ultrasonic cleaning and Cement Composite Treated by Plasma, the polypropylene mesh that preparation is clean.
Step 2: fibroin coating liquid preparation: Bombyx bombycis is removed pupa, and the sodium bicarbonate with 1% ~ 5% was handled 30 ~ 90 minutes down at 90-100 ℃, afterwards clean with rinsed with deionized water; Repeat above-mentioned cleaning of removing the Bombyx bombycis behind the pupa 1 ~ 3 time, to remove sericin fully; After to be cleaned, with take out behind the sericin Bombyx bombycis 65 ℃ dry 24 hours down, obtain boiled silk, and boiled silk be dissolved in the lithium-bromide solution of 7 ~ 10mol/L under 30 ~ 60 ℃ of conditions, remove undissolved coming unstuck to filter; After 30 ~ 90 minutes; It is in 10000 ~ 14000 the cellulose bag filter that the lithium-bromide solution of boiled silk is poured into residual molecular weight; And, prepare the regenerated silk protein solution, and dilute the 5% regenerated silk protein solution that obtains with deionized water with deionized water dialysis 3 days.
Step 3: fibroin coating polypropylene mesh preparation: the polypropylene mesh of the cleaning that obtains in the step 1 inserted in 5% the regenerated silk protein solution that obtains in the step 2 fully soak into, carry out the regenerated silk protein solution on the surface of polypropylene mesh and apply; Took out after drying in 20 hours; Repeat the regenerated silk protein solution coating procedure four times of above-mentioned polypropylene mesh, place and handled in 90% ethanol 20 ~ 40 minutes, dry back obtains fibroin coating polypropylene mesh.
Fibroin coating polypropylene mesh that the present invention is made and common polypropylene mesh without the silk rolling protein coating are observed, and contrast is found:
As shown in Figure 2, in Fig. 1, ganoid undressed polypropylene mesh, the surface-coated of fibroin coating polypropylene mesh of the present invention layer of even fibroin coating.And as shown in Figure 2, the fibroin coating is full of polypropylene fibre surface and fiber junction, and the polypropylene mesh hole do not seal, and is opening.
After treating that the preparation of said fibroin polypropylene mesh is accomplished, with fibroin coating polypropylene mesh with needs cutting typing encapsulation after, the ethane via epoxyethane sterilization, subsequent use.
Fibroin coating polypropylene mesh of the present invention; Applied one deck fibroin coating in showing of polypropylene mesh; It has kept the high porosity of polypropylene mesh; It utilizes the characteristics that the polypropylene mesh porosity is high, the fine title deed for land of surrounding tissue ability is gone into growth on the one hand, strengthens the stretch-proof performance of repairing the position; Utilize proteic biocompatibility of regenerated silk and the effect that promotes fibroblastic growth on the other hand, healing acceleration reduces complication, and the present invention can be used for clinical female pelvic reconstructive surgery, effectively reduces the generation of Reconstruction infectious-related complication at the bottom of the basin.And in the preparation process, the present invention does not relate to any poisonous and harmful reagent, and the follow-up use of medical patch is had no adverse effect, and has guaranteed medical safe property.
Specify fibroin coating polypropylene mesh of the present invention and no fibroin polypropylene mesh as the medical beneficial effect of timbering material through experiment below.
Experiment: rabbit abdominal-wall defect zoopery:
Experimental model: 6 of the female White Rabbits of cleaning level New Zealand, body weight 2.3 ~ 2.7kg, the about 2.5kg of average weight, rabbit is 3 months age.
6 new zealand white rabbits are divided into 3 groups at random, are respectively 30 days groups, 60 days groups, 90 days groups, 2 every group.According to implanting whether coated wire protein coating of polypropylene mesh, the net sheet is divided into two groups of A, B, every group each 12.Wherein, embodiment of the invention group (A group): fibroin coating polypropylene mesh is arranged; Comparative Examples group (B group): no fibroin coating polypropylene mesh.
A,
B implants the cardinal principle specimen observation that net sheet different time is drawn materials for two groups:
It is dead that the net sheet is implanted the no laboratory animal in back, do not see that all intraperitoneal is tolerant from the bulging of abdominal-wall defect place for two groups, and all net sheets intraperitoneal all do not take place corrode and expose, and do not have local abscess and form.The net sheet is sprawled smooth, does not see obvious distortion before implanting.All form the loose connective tissue layer of thin layer between two networking sheets surface and stomach wall subcutaneous tissue.
Implant the HE dyeing observation that net sheet different time is drawn materials for two groups:(HE dyeing: hematoxylin-eosin staining method, hematoxylin-eosin staining):
When (1) the net sheet is implanted 30 days, there is more inflammatory cell to assemble around the visible embedded material.Along with the increase of the time of implantation, inflammatory cell quantity obviously reduces around the net sheet; Fibroblast quantity increases, and form is also gradually ripe; Collagen secretion increases.All see more inflammatory response cell, plasma cell around the fibril of no fibroin coating networking sheet, the macrophage amount is few than inflammatory cell, simultaneously visible minute quantity multinucleated giant cell.That have in addition can be in net sheet peripheral vessels the leukocyte of volume.Have the inflammatory reaction and the fiberization of fibroin coated mesh sheet group (A group) all lighter, the new vessels generation on every side of net sheet is apparent in view, and it is many that macrophage quantity does not have fibroin coated mesh sheet.
When (2) the net sheet is implanted 60 days, still visible inflammatory response cell around the two networking sheets, but obviously reduce when implanting 30 days, macrophage quantity also reduces, and visible a small amount of eosinophilic granulocyte and basophilic granulocyte; And the net sheet increases the weight of when fiberization is implanted 30 days on every side.Part does not have the still visible lymphocytic infiltrations of fibroin coated mesh sheet (B group) on every side.
When (3) the net sheet is implanted 90 days, almost lose inflammatory response cell around each networking sheet; Macrophage still exists, but quantity further reduces.Have fibroin coated mesh sheet (A group) on every side fiberization not have fibroin coated mesh sheet (B group) light.The reaction of net sheet peripheral vessels is more obvious when implanting 30 days and 60 days.At this moment, all net sheets all show the favorable tissue compatibility, net sheet and surrounding tissue good knitting.
Implant the Masson dyeing observation that net sheet different time is drawn materials for two groups:
Two groups of collagen stainings (Masson dyeing) of implanting the net sheet show: for two networking sheets; When A group fibroin coated mesh sheet of the present invention (Fig. 3) and B group contrast networking sheet (Fig. 4) are implanted 30 days; After two networking sheets are implanted; More inflammatory cell is assembled around the fibril of polypropylene mesh, is with sparse on a small quantity new life's collagen tissue outward.The net sheet is implanted (Fig. 5 after 90 days; Fig. 6); Rarely seen a spot of inflammatory response cell around the fibril of fibroin coating polypropylene mesh (A group) is arranged; Macrophage is also less; Visible obviously collagen tissue holds around the fibril; Net sheet and surrounding tissue good knitting, not having fibroin coating polypropylene mesh (B group) then has more inflammatory response cell and macrophage on every side and does not have tangible collagen tissue and surround, and itself and surrounding tissue amalgamation are relatively poor.Therefore, it is many that the present invention has the newborn collagen quantity around the fibroin coated mesh sheet not have fibroin coated mesh sheet, and its biocompatibility with tissue is better.
Implant the transmission electron microscope observing that net sheet different time is drawn materials for two groups:
When the net sheet was implanted 30 days, more inflammatory cell held around the net sheet, and fibrosis forms not obvious.The net sheet is implanted the more preceding minimizing of inflammatory cell during with 90 days in 60 days, comparison diagram 7 and Fig. 8, and than the net sheet of no fibroin coating, embodiment of the invention fibroin coating polypropylene mesh periphery fiberization is stronger, and the fibroblast arrangement is orderly.
Fig. 9 and Figure 10 are embodiment group and Comparative Examples networking sheet 30 days, and 60 days, 90 days tensile stress-strain figures, vertical coordinate were represented tensile stress at break (MPa), the abscissa elongation strain (%) of representing to rupture.
Visible along with implanting time lengthening among the figure, tensile stress at break, fracture elongation strain, and tensile stress totally presents increase tendency during disconnected taking off.The fibroin coating polypropylene mesh of embodiment group all is higher than the no fibroin coating polypropylene mesh of the Comparative Examples group of the same period at 30 days, 60 days, 90 days tensile stress at break.The fracture elongation strain parameter of fibroin coating polypropylene mesh of the present invention is obvious with the trend that the implantation time lengthening increases, and is not obvious at the increase tendency of no fibroin coating polypropylene mesh.
Show that from the above experimental diagrams of respectively organizing fibroin coating polypropylene mesh of the present invention utilizes the characteristics that polypropylene sticking patch porosity is high, surrounding tissue is cut growth well on the one hand, can strengthen the stretch-proof performance of repairing the position; Utilize the proteic biocompatibility of regenerated silk on the other hand, increase the biocompatibility of implant and tissue, reduce the generation of post-operative complication.
The present invention shows through the zoopery result; The present invention is applied to department of obstetrics and gynecology female pelvic reconstruction operations and surgery abdominal hernia repairing operation as timbering material; The polypropylene mesh of fibroin coating can reduce early stage inflammatory reaction of implant site and fibrosis; Reduce the tissue adhesion; Increase biocompatibility; Reduce the generation of net sheet related complication, and fibroin coating polypropylene mesh has good relatively tensile stress and elongation strain is answered, and helps the growth of female pelvic tissue.
More than specific embodiment of the present invention is described in detail, but it is just as example, the present invention is not restricted to the specific embodiment of above description.To those skilled in the art, any equivalent modifications that the present invention is carried out with substitute also all among category of the present invention.Therefore, not breaking away from impartial conversion and the modification of being done under the spirit and scope of the present invention, all should contain within the scope of the invention.
Claims (10)
1. a fibroin coating polypropylene mesh is characterized in that, comprises the polypropylene mesh as support; On said polypropylene mesh surface, adopt fibroin coating liquid parcel one deck fibroin coating; And the hole of said polypropylene mesh is the unlatching shape.
2. method according to claim 1 is characterized in that, in the said fibroin coating liquid, the volumetric concentration of fibroin is 1 ~ 8%.
3. the method for preparing of a fibroin coating polypropylene mesh as claimed in claim 1 is characterized in that, may further comprise the steps:
The preparation silk protein solution; And with polypropylene mesh in being soaked in said silk protein solution more than 6 hours, fully soak into, thereby on polypropylene mesh, apply one deck fibroin coating;
With the polypropylene mesh that scribbles the fibroin coating after soaking into take out, drying, and be positioned over and soak 20 ~ 40 minutes after drying in the ethanol, make fibroin coating polypropylene mesh.
4. method according to claim 3 is characterized in that, repeat polypropylene mesh be soaked in fully soak in the said silk protein solution with drying steps repeatedly after, more dried polypropylene mesh is soaked in the alcoholic solution.
5. the application of a fibroin coating polypropylene mesh as claimed in claim 3 is characterized in that, the mass concentration of said alcoholic solution is 60 ~ 90%.
6. method according to claim 3 is characterized in that, also comprises the preparation process of silk protein solution, the steps include: with Bombyx bombycis remove pupa successively, come unstuck handle after, dissolving; And the solution that obtains is carried out deionized water dialyse, obtain the regenerated silk protein solution; Dilute said regenerated silk protein solution, obtain volumetric concentration and be 1 ~ 8% fibroin coating liquid.
7. method according to claim 6 is characterized in that, Bombyx bombycis goes coming unstuck behind the pupa to comprise in handling, and earlier Bombyx bombycis is placed 1 ~ 5% sodium bicarbonate to handle under 90 ~ 100 ℃ of conditions 30 ~ 90 minutes, takes out, and cleans with deionized water; Behind repetition said process 1 ~ 3 time, drying is 30 ~ 90 minutes under 60 ~ 70 ℃, obtains boiled silk; Afterwards under 30 ~ 60 ℃ of conditions, boiled silk will be dissolved in the lithium-bromide solution of 7 ~ 10mol/L and handle 30 ~ 90 minutes.
8. method according to claim 3 is characterized in that, in said Bombyx bombycis deionized water dialysis procedure; Will; To be dissolved in silkworm silk solution in the lithium bromide, to pour residual molecular weight into be in 10000 ~ 14000 the fiber bag filter, with deionized water dialysis 2 ~ 3 days, thereby to obtain fibroin coating liquid.
9. method according to claim 3; It is characterized in that also comprise the cleaning course of putting into said silk protein solution polypropylene mesh before, its step does; After earlier polypropylene mesh being carried out ultrasonic cleaning, Cement Composite Treated by Plasma, obtain clean polypropylene mesh.
10. the application like fibroin coating polypropylene mesh as described in above-mentioned arbitrary claim is characterized in that, said fibroin coating polypropylene mesh is as the timbering material of reconstructive surgery at the bottom of the women basin.
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| CN114366850A (en) * | 2022-01-19 | 2022-04-19 | 复旦大学附属华山医院 | Artificial rotator cuff patch based on drug-loaded silk protein and preparation method thereof |
| CN114392396A (en) * | 2022-01-19 | 2022-04-26 | 福州市第二医院（福建省福州中西医结合医院、福州市职业病医院） | Rotator cuff patch based on silk fibroin gel and preparation method thereof |
| CN114392396B (en) * | 2022-01-19 | 2022-10-28 | 福州市第二医院（福建省福州中西医结合医院、福州市职业病医院） | Rotator cuff patch based on silk fibroin gel and preparation method thereof |
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