CN101195043A - Medical patch with different bioavailability on both sides and method for preparing the same - Google Patents
Medical patch with different bioavailability on both sides and method for preparing the same Download PDFInfo
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- CN101195043A CN101195043A CNA2007101718245A CN200710171824A CN101195043A CN 101195043 A CN101195043 A CN 101195043A CN A2007101718245 A CNA2007101718245 A CN A2007101718245A CN 200710171824 A CN200710171824 A CN 200710171824A CN 101195043 A CN101195043 A CN 101195043A
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Abstract
The invention belongs to the medical material technical field, in particular to a medical sticking patch and preparing process, and the medical sticking patch has two sides which are respectively provided with different biocompatibilities. The invention uses plasma to treat the medical sticking patch for improving the infiltrating performance, and the coating of regenerative silk fibroin is bonded to enhance the biocompatibility of the medical sticking patch. The whole modifying process is simple and easy to operate, does not relate to any toxic and noxious reagents, provides no unfavorable influence to the subsequent utilization of the medical sticking patch, and can substantially lower the adhesion produced between the medical sticking patch and body tissues when in the using process. On one hand, the medical sticking patch prepared by the invention uses the characteristics of a high porosity factor of the polypropylene category sticking patch and the well-entering growth of peripheral tissues, and can reinforce the tensile performance of repairing locations, and on the other hand, the invention uses the biocompatibility of regenerative silk fibroin, and can effectively lower the occurrence of complications such as tissue adhesion.
Description
Technical field
The invention belongs to surface physics and biological technical field, be specifically related to a kind of method of utilizing the natural biological macromole to modify medical material.
Background technology
Silkworm silk is human widely used natural biological macromole, is a kind of protein fibre of being made up of fibroin albumen (70-80%) and sericin, is widely used in textile industry.Because have excellent biological compatibility, no antigen, suitable characteristics such as biodegradability simultaneously based on the silk fiber of fibroin albumen, silk fiber also is widely used in the clinical medicine as operation suture thread.Along with the development of protein chemistry, tissue engineering, biochemistry etc., fibroin albumen also is widely used in fields such as medicine, food, fine chemistry industry, organizational project as tissue engineering material, medicine controlled release carrier, intelligent aqueous gel capable, medical dressing, bio-modification pick off etc.The inventor once used the highly sensitive enzyme electrode of regenerated silk fibroin formulations prepared from solutions, medicine controlled releasing Nano microsphere, tissue engineered porous scaffold material.
Tension-free hernioplasty is the advanced technology of treatment hernia, the sticking patch that uses has polypropylene sticking patch (PP mesh), expanded polytetrafluoroethylsealing sticking patch (e-PTFE mesh) and polyester patches (PET mesh) etc. at present, and wherein the use with tension-free polypropylene sticking patch is the most extensive.Use tension-free polypropylene sticking patch can effectively treat adult's oblique hernia, straight hernia etc., treat the misery that the back relapse rate is low, alleviate patient, but the early stage out-of-bed activity of postoperative.But the use of polypropylene sticking patch can cause the adhesion of peritoneum.From the histology, foreign body reaction is arranged and form with the multinuclear foreign-body giant cell along the polypropylene sticking patch in the animal experiment, the sticking patch two sides is peritoneumization all.After hyaluroni, hydroxy methocel carry out finishing, can reduce the generation of adhesion, but there is dispute in its effect.
Plasma is partially ionized gas, is made up of electronics, ion, free radical, neutral particle and radiation photon etc.Low temperature plasma glow discharge is even discharge, and wherein particle energy density is suitable, can not cause mechanical damage to fiber at low temperatures, is applicable to the surface modification of fiber.Hydrophilic, wettability that the rear surface is handled in use such as polypropylene, polyester fiber low-temperature plasma sputter glow discharge obviously improve.
Fibroin albumen is made up of 18 seed amino acids such as glycine, alanine, have excellent biological compatibility and adjustable biodegradability, be used for coated titanium mesh and repair that tracheal injury can be induced fibroblast and the mucosa cell is good in the fibroin protein film superficial growth, no granulation tissue hyperplasia, the generation of healing acceleration, minimizing complication.
At present, adopt the means of finishing very extensive in the tissue engineering material Application for Field to improve properties of materials.Utilize ion sputtering at its surface deposition hydroxyapatite layer as titanium alloy material, can improve its biocompatibility greatly, help osteoblast in its surface differentiation, propagation, and can induce the osseous tissue hypertrophy.Utilize collagen protein modified polymer material also can significantly improve its biocompatibility.Utilize hyaluronate or hydroxy methocel finishing polypropylene sticking patch may reduce the generation of complication such as tissue adhesion, but, use to be subjected to strict restriction owing to collagen protein, hyaluronic acid etc. separate purification difficult, the source is limited, price is high.
Based on above situation, the present invention utilizes regenerated silk fibroin good biocompatibility, wide material sources, lower-price characteristic, regenerated silk fibroin is applied to the modification of medical patch by the modern physics method, improved the biocompatibility of medical patch, improve the therapeutic effect of herniorrhaphy, solved the problem of complication such as tissue adhesion in the herniorrhaphy.
Summary of the invention
The object of the present invention is to provide a kind of by Cement Composite Treated by Plasma, and the medical patch of modifying with the regenerated silk fibroin single face and preparation method thereof.
The method with regenerated silk fibroin modification medical patch that the present invention proposes with low-temperature plasma sputter medical patch, to improve its wettability, makes the hydrophilic and hydrophobic on medical patch two sides produce significant difference; Reuse regenerated silk fibroin solution impregnation applies; Handle with ethanol water at last, promptly obtain the medical patch that the single face regenerated silk fibroin is modified.
In said method, the medical patch that uses is polypropylene sticking patch or polyester patches.
In said method, used regenerated silk protein solution is that the dialysis after come unstuck, neutral salt dissolves of Bombyx bombycis silk prepares, and concentration is 1-5%.
In said method, the condition of plasma sputtering is controlled to be: voltage is that 30-50V, electric current are 1-2A, and the processing time is 2-20 minute.
In said method, the infiltrating time of regenerated silk fibroin solution is 12-24 hour.
In said method, ethanol water concentration is 60-90%, processing time 20-40 minute.
The operating procedure of said method is as follows: Bombyx bombycis removes pupa, handles 30-90 minute down at 90-100 ℃ with sodium carbonate or the sodium bicarbonate of 1-5%, and is clean with rinsed with deionized water, repeats 1-3 time on demand to remove sericin fully; Drying is 24 hours under 65 ℃, obtains boiled silk.The lithium-bromide solution of boiled silk with 7-10mol/L dissolved 30-90 minute down at 30-60 ℃.Filter and remove undissolved degumed silk, it is 10 that solution pours into molecular cut off, 000-14, in 000 the cellulose bag filter, with deionized water dialysis 2-3 days, prepare regenerated silk fibroin solution, the concentration of regulating regenerated silk fibroin solution with deionized water is 1-5%.
With commodity medical patch Low Temperature Plasma Treating, be infiltrated on then in the regenerated silk fibroin solution of 1-5%, dip-coating 12-24 hour, take out the back air drying.Modified medical patch immerses in the 60-90% ethanol water again to be handled 20-40 minute, promptly got the medical patch that fibroin albumen is modified.
The medical patch sem observation that the regenerated silk fibroin of above-mentioned preparation is modified, can see through plasma sputtering face polypropylene fibre infall having fibroin protein film to form, but not have fibroin protein film to form without plasma sputtering face polypropylene fibre.Animal experiment shows: it agrees with good with regenerated silk modification face and surrounding tissue growth this medical patch in the abdominal-wall defect reparation; And modify face in abdominal-wall defect is repaired through regenerated silk fibroin, and the adhesive area between nethike embrane and abdominal visceral browser reduces to 5.82% by 98.3%; And the adhesion degree obviously reduces, and forms loose adhesion only and between nethike embrane.
Do not see and similar techniques of the present invention.
Description of drawings
Fig. 1 electromicroscopic photograph of (b) that is medical patch before regenerated silk fibroin is modified after (a).
The adhesion area of Fig. 2 (a) back (b) that is medical patch before regenerated silk fibroin is modified and adhesion situation contrast to be schemed.
Fig. 3 is the adhesion situation contrast figure of medical patch before and after regenerated silk fibroin is modified that the abdominal wall at sites optical photograph shows in the animal experiment.Wherein (A) is not modified, (B) for modifying face.
The specific embodiment
Below utilize embodiment to further describe the present invention, but can not think to limit scope of invention.
Embodiment 1:
With boiling 45 minutes in 20 gram Bombyx bombycis silks immersion 2L, 0.5% sodium carbonate liquor, clean with deionised water.Repeat above operation once, the Bombyx bombycis silk after will coming unstuck is 65 ℃ of following dry for standby.
Take by weighing the dried Bombyx bombycis silk that comes unstuck of 10 grams and be dissolved in the lithium-bromide solution that 100ml concentration is 9.5mol/L, dissolved 45 minutes down at 40 ℃.Filter to remove and not dissolve the Bombyx bombycis silk, solution pours in the cellulose bag filter, with deionized water dialysis three days, concentration is 4.70% regenerated silk fibroin solution.
Get commercial polypropylene sticking patch voltage 40V, electric current be under the 2.5A argon atmospher ionic medium sputter 2 minutes, immersed immediately in 4.7% the regenerated silk fibroin solution 24 hours.Taking-up was dried in air 12 hours, be dipped in 70% ethanol water 30 minutes, the polypropylene for medical article sticking patch modified of regenerated silk fibroin.
Animal experiment shows: agree with good with regenerated silk modification face and surrounding tissue growth in the abdominal-wall defect reparation; And modify face through regenerated silk fibroin, and the adhesive area between nethike embrane and abdominal visceral browser reduces to 5.82% by 100%; And the adhesion degree obviously reduces, and forms loose adhesion only and between nethike embrane, and does not stick together between internal organs such as intraperitoneal small intestinal.
Embodiment 2:
With boiling 45 minutes in 20 gram Bombyx bombycis silks immersion 2L, 0.5% sodium carbonate liquor, clean with deionised water.Repeat above operation once, the Bombyx bombycis silk after will coming unstuck is 65 ℃ of following dry for standby.
Take by weighing the dried Bombyx bombycis silk that comes unstuck of 10 grams and be dissolved in the lithium-bromide solution that 100ml concentration is 9.5mol/L, dissolved 45 minutes down at 40 ℃.Filter to remove and not dissolve the Bombyx bombycis silk, solution pours in the cellulose bag filter, with deionized water dialysis three days, concentration is 4.70% regenerated silk fibroin solution.
Get commercial polypropylene sticking patch voltage 40V, electric current be under the 2.5A argon atmospher ionic medium sputter 10 minutes, immersed immediately in 2% the regenerated silk fibroin solution 18 hours.Taking-up was dried in air 12 hours, be dipped in 60% ethanol water 30 minutes, the polypropylene for medical article sticking patch modified of regenerated silk fibroin.
Animal experiment shows: agree with good with regenerated silk modification face and surrounding tissue growth in the abdominal-wall defect reparation; And modify face through regenerated silk fibroin, and the adhesive area between nethike embrane and abdominal visceral browser reduces to 4.5% by 98.3%; And the adhesion degree obviously reduces, and forms loose adhesion only and between nethike embrane, and does not stick together between internal organs such as intraperitoneal small intestinal.
Embodiment 3:
With boiling 45 minutes in 20 gram Bombyx bombycis silks immersion 2L, 0.5% sodium bicarbonate solution, clean with deionised water.Repeat above operation once, the Bombyx bombycis silk after will coming unstuck is 65 ℃ of following dry for standby.
Take by weighing the dried Bombyx bombycis silk that comes unstuck of 10 grams and be dissolved in the lithium-bromide solution that 100ml concentration is 9.5mol/L, dissolved 45 minutes down at 40 ℃.Filter to remove and not dissolve the Bombyx bombycis silk, solution pours in the cellulose bag filter, with deionized water dialysis three days, concentration is 4.70% regenerated silk fibroin solution, deionized water is regulated to such an extent that concentration is 2% silk fibroin protein solution.
Get commercial polyester patches voltage 50V, electric current be under the 1.5A argon atmospher ionic medium sputter 10 minutes, immersed immediately in 4.7% the regenerated silk fibroin solution 24 hours.Taking-up was dried in air 12 hours, be dipped in 80% ethanol water 40 minutes, the regenerated silk fibroin medical polyester patches of modifying.
Animal experiment shows: agree with good with regenerated silk modification face and surrounding tissue growth in the abdominal-wall defect reparation; And modify face through regenerated silk fibroin, and the adhesive area between nethike embrane and abdominal visceral browser reduces to 5.1% by 96.6%; And the adhesion degree obviously reduces, and forms loose adhesion only and between nethike embrane, and between internal organs such as intraperitoneal small intestinal any adhesion does not take place.
Embodiment 4:
With boiling 45 minutes in 20 gram Bombyx bombycis silks immersion 2L, 0.5% sodium bicarbonate solution, clean with deionised water.Repeat above operation once, the Bombyx bombycis silk after will coming unstuck is 65 ℃ of following dry for standby.
Take by weighing the dried Bombyx bombycis silk that comes unstuck of 10 grams and be dissolved in the lithium-bromide solution that 100ml concentration is 9.5mol/L, dissolved 45 minutes down at 40 ℃.Filter to remove and not dissolve the Bombyx bombycis silk, solution pours in the cellulose bag filter, with deionized water dialysis three days, concentration is 4.70% regenerated silk fibroin solution, regulate to such an extent that concentration is 2% regenerated silk fibroin solution with deionized water.
Get commercial polyester patches voltage 50V, electric current be under the 1.5A argon atmospher ionic medium sputter 20 minutes, immersed immediately in 2% the regenerated silk fibroin solution 12 hours.Taking-up was dried in air 12 hours, be dipped in 80% ethanol water 20 minutes, the regenerated silk fibroin medical polyester patches of modifying.
Animal experiment shows: agree with good with regenerated silk modification face and surrounding tissue growth in the abdominal-wall defect reparation; And modify face through regenerated silk fibroin, and the adhesive area between nethike embrane and abdominal visceral browser reduces to 4.6% by 97.6%; And the adhesion degree obviously reduces, and forms loose adhesion only and between nethike embrane, and does not stick together between internal organs such as intraperitoneal small intestinal.
Claims (2)
1. a two sides has the preparation method of the medical patch of different bioavailability, it is characterized in that concrete steps are as follows: with low-temperature plasma sputter medical patch, to improve its wettability, make the hydrophilic and hydrophobic on medical patch two sides produce significant difference; Reuse regenerated silk fibroin solution impregnation applies; Handle with ethanol water at last, promptly obtain the medical patch that the single face regenerated silk fibroin is modified; Wherein:
Described medical patch is polypropylene sticking patch or polyester patches;
Used regenerated silk protein solution is that the dialysis after come unstuck, neutral salt dissolves of Bombyx bombycis silk prepares, and concentration is 1-5%;
The condition of described plasma sputtering is: voltage is that 30-50V, electric current are 1-2A, and the processing time is 2-20 minute;
The infiltrating time of described regenerated silk fibroin solution is 12-24 hour;
Described ethanol water concentration is 60-90%, processing time 20-40 minute.
2. medical patch by the preparation of the described method of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101718245A CN101195043A (en) | 2007-12-06 | 2007-12-06 | Medical patch with different bioavailability on both sides and method for preparing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101718245A CN101195043A (en) | 2007-12-06 | 2007-12-06 | Medical patch with different bioavailability on both sides and method for preparing the same |
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| CN101195043A true CN101195043A (en) | 2008-06-11 |
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| CNA2007101718245A Pending CN101195043A (en) | 2007-12-06 | 2007-12-06 | Medical patch with different bioavailability on both sides and method for preparing the same |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102085173A (en) * | 2011-01-26 | 2011-06-08 | 上海交通大学 | Bioactive emulsion with natural fibroin as emulsifier and preparation method thereof |
| CN102302801A (en) * | 2011-09-14 | 2012-01-04 | 上海市第六人民医院 | Silk-fibroin-coated polypropylene mesh and preparation method thereof |
| WO2012100574A1 (en) * | 2011-01-27 | 2012-08-02 | 苏州大学 | High-strength biological scaffold material and preparation method thereof |
| CN104288836A (en) * | 2014-09-09 | 2015-01-21 | 上海纳米技术及应用国家工程研究中心有限公司 | Active coating layer modified polyethylene terephthalate material and preparation method thereof |
| CN111134747A (en) * | 2019-12-31 | 2020-05-12 | 南通纺织丝绸产业技术研究院 | Barb type silk suture line and preparation method thereof |
| CN111407924A (en) * | 2020-04-17 | 2020-07-14 | 南京鼓楼医院 | Composite patch with anisotropic surface and preparation method and application thereof |
-
2007
- 2007-12-06 CN CNA2007101718245A patent/CN101195043A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102085173A (en) * | 2011-01-26 | 2011-06-08 | 上海交通大学 | Bioactive emulsion with natural fibroin as emulsifier and preparation method thereof |
| CN102085173B (en) * | 2011-01-26 | 2012-05-30 | 上海交通大学 | Bioactive emulsion with natural fibroin as emulsifier and preparation method thereof |
| WO2012100574A1 (en) * | 2011-01-27 | 2012-08-02 | 苏州大学 | High-strength biological scaffold material and preparation method thereof |
| US10201410B2 (en) | 2011-01-27 | 2019-02-12 | Soochow University | High-strength biological scaffold material and preparation method thereof |
| CN102302801A (en) * | 2011-09-14 | 2012-01-04 | 上海市第六人民医院 | Silk-fibroin-coated polypropylene mesh and preparation method thereof |
| CN102302801B (en) * | 2011-09-14 | 2014-04-02 | 上海市第六人民医院 | Silk-fibroin-coated polypropylene mesh and preparation method thereof |
| CN104288836A (en) * | 2014-09-09 | 2015-01-21 | 上海纳米技术及应用国家工程研究中心有限公司 | Active coating layer modified polyethylene terephthalate material and preparation method thereof |
| CN111134747A (en) * | 2019-12-31 | 2020-05-12 | 南通纺织丝绸产业技术研究院 | Barb type silk suture line and preparation method thereof |
| CN111407924A (en) * | 2020-04-17 | 2020-07-14 | 南京鼓楼医院 | Composite patch with anisotropic surface and preparation method and application thereof |
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Application publication date: 20080611 |