CN102302210A - Beverage with anti-fatigue effect - Google Patents
Beverage with anti-fatigue effect Download PDFInfo
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- CN102302210A CN102302210A CN201110203007A CN201110203007A CN102302210A CN 102302210 A CN102302210 A CN 102302210A CN 201110203007 A CN201110203007 A CN 201110203007A CN 201110203007 A CN201110203007 A CN 201110203007A CN 102302210 A CN102302210 A CN 102302210A
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Abstract
The invention belongs to the technical field of foods and discloses a beverage made from Cordyceps polysaccharides, taurine and/or medlar polysaccharides. The beverage is characterized by comprising 0.1-2 parts by weight of Cordyceps polysaccharides and 0.5-10 parts by weight of taurine and/or medlar polysaccharides. The pharmacological studies prove that the raw materials of the beverage have good anti-fatigue effect.
Description
Technical field
The invention belongs to food technology field, be specifically related to contain in a kind of raw material the beverage of Cordyceps sinensis polysaccharide, taurine.
Background technology
Along with the quickening of modern life rhythm, the aggravation of social competition, much human is because the pressure etc. of work, study, and fatigue becomes the health problem of puzzlement much human.
1, why the people can be tired
Fatigue is a kind of physiological phenomenon, is a kind of mechanism of protectiveness to the people.This is that health sends the signal that have a rest to us.If no matter ignore, health will suffer damage, and is overworked for a very long period at last or disease to it.
2, Pi Lao classification fatigue generally can be divided into physical fatigue, mental fatigue, mental fatigue and pathology fatigue.(1) physical fatigue.Physical fatigue makes corporality tired again; Be since when the people for a long time, greatly during the physical exertion campaign of intensity; Produced a large amount of metabolins in the body; Like lactic acid, carbon dioxide, serum urea nitrogen etc.; This type material gathers in vivo; Stimulate human tissue cell and nervous system, will make the people produce sense of fatigue.(2) mental fatigue.Mental fatigue is after people require mental skill for a long time; Cause what cerebrum blood and oxygen supply deficiency caused; Specifically can occur absent minded, feel dizzy, slow in reacting, limbs fatigue or symptom such as drowsiness, serious caused insomnia, dreaminess, feel sick, problems such as vomiting, personality change.(3) mental fatigue.Mental fatigue is the longlyest in the modern life to see and complicated a kind of fatigue, because the anxiety of continuation and pressure generation.The people is engaged in work, the learning activities of some dullnesses, machinery for a long time, and maincenter local nerve cell causes the people that enthusiasm and the interest of work to life is obviously reduced, until producing weary mood owing to suppressing appears in constant tension.Produce the people of mental fatigue, detesting appears in the lighter, the symptom of malinger goldbrick relinquish, study, life, and weight person depression, neurasthenia, compulsive behavior also can occur and such as the phenomenon of habits and customs changes such as beginning smoking, excessive drinking.(4) pathology is tired.Pathology fatigue is because symptom such as human body that some disease caused is weak, unable.Fatigue is one of these sick tendencys.All can make the patient feel indescribable fatigue such as virus hepatitis, pulmonary tuberculosis, diabetes, myocardial infarction, anaemia, blood disease and cancer etc.; This fatigue and muscle power, mental, psychological fatigue property is different fully, it has three characteristics: the one, when healthy subjects should not fatigue occur, occur; The 2nd, tired degree is serious, eliminates also slowly; The 3rd, this fatigue is often with other symptoms, like low-heat, general malaise, poor appetite or hyperfunction etc.Only behind disease cured, fatigue just can be eliminated.(5) comprehensive fatigue.The often not single reason of the fatigue that the modern life causes causes; The reason of its existing muscle power, mentality; The reason that psychology, social activity are also arranged; The reason that also possibly also be mingled with disease; Make " symptom " of various single fatigues not really outstanding and typical, the fatigue that this non-single factors causes is called " comprehensive fatigue ".
Health is in fatigue state for a long time, can cause hormone in vivo metabolism disorder, unusual, the hypoimmunity of nervous system regulatory function, can cause vegetative dystonie shapes such as shoulder is ached, headache.The people that symptom is strong, how many chance of suffering from respiratory tract, digestive system, circulator official rank infection disease than common people is.The people who suffers from diseases such as blood vessel, heart if overwork at ordinary times, possibly cause sudden death.
Summary of the invention
For these reasons, the inventor is through experimental study, with Cordyceps sinensis polysaccharide, taurine and/or LBP-X combination; And be prepared into beverage, pharmacological evaluation shows, the beverage of the present invention's preparation; The effect that not only has conventional beverage, the colleague also has antifatigue effect.
The present invention realizes through following technical proposals.
A kind of beverage with antifatigue effect, raw material is Cordyceps sinensis polysaccharide, taurine and/or LBP-X in the beverage, wherein Cordyceps sinensis polysaccharide 0.1-2 weight portion, taurine and/or LBP-X 0.5-10 weight portion.
Wherein can also add set-in of plum rains juice in the beverage.
Wherein can also add rose in the beverage, glucose and/or oligoisomaltose
Wherein can also add Pandan Leaves juice in the beverage, glucose and/or oligoisomaltose.
Wherein plum juice refers to prune juice, and dark plum juice is stewed plum juice, carving plum juice, and a kind of of plum juice wrapped up in Soviet Union
Cordyceps sinensis polysaccharide can perhaps must be got according to prior art for preparing by commercially available acquisition in the above-mentioned beverage, perhaps prepares according to following method: bacterial classification is: Paecilomyces hepiali chen or Hirsutella hepiali Chen et Shen; Culture medium is: glucose 20-40 weight portion, serpentgrass 30-100 weight portion, milk powder 20-30 weight portion, dusty yeast 20-50 weight portion, magnesium sulfate 0.2-5 weight portion, dipotassium hydrogen phosphate 0.2-5 weight portion; The serpentgrass water extracts the back and filters, and use the distilled water constant volume after in filtrating, adding other component mixing while hot, and adjust pH is to 5-6.5, and cultivation is 3-10 days under 18-25 ℃, and the secondary feed supplement is: glucose and/or wolfberry fruit extract; After cultivating end, ferment cordyceps sinensis bacterium liquid is filtered, centrifugal, get supernatant, place 30-90 ℃ of bath, regulate the pH value to 4-6, add proteolytic enzyme and cellulolytic enzyme respectively and carry out enzymolysis, move into the enzyme that goes out in 100 ℃ of baths after enzymolysis 80-200 minute immediately; In 80 ℃ of water-baths, it is condensed into the 1/6-1/3 of volume, adds the ethanol of the 20%-95% of 3-6 times of volume after the cooling while stirring, then at 4-8 ℃ of static 12-36 hour; Centrifugal; Concentrate, wash back 60 ℃ of constant temperature dryings, both got Cordyceps sinensis polysaccharide with absolute ethyl alcohol.
One, beverage raw material screening test
Test 1 group: raw material is Cordyceps sinensis polysaccharide 0.5g, taurine 2g, 3g.
Test 2 groups: raw material is Cordyceps sinensis polysaccharide 0.5g, taurine 2g, panaxan 3g.
Test 3 groups: raw material is Cordyceps sinensis polysaccharide 0.5g, taurine 2g, people's astragalus polyose 3g.
Test 4 groups: Cordyceps sinensis polysaccharide 0.5g, taurine 2g.
Test 5 groups: Cordyceps sinensis polysaccharide 0.5g, LBP-X 2g.
Test 6 groups: Cordyceps sinensis polysaccharide 0.5g, taurine and LBP-X 2g.
Above-mentioned raw materials adds distilled water, filters, and sterilization is prepared into bottled drink.
Hot test:
It is that 40 ℃ ± 2 ℃ insulating boxs were placed 10 days that beverage is placed temperature, difference sampling and measuring in the time of 5 days, 10 days.Result of the test is seen table 1.
The high light experiment:
Placing illumination is the lighting box 10 days of 4500lx ± 500lx, sampling and measuring respectively in the time of 5 days, 10 days.Result of the test is seen table 2.
Table 1 hot test result
Table 2 highlight test result
Result of the test: show that through high temperature, high highlight test Cordyceps sinensis polysaccharide and taurine and/or LBP-X can access the qualified product of product quality as beverage raw material, therefore, above-mentioned raw materials is significant.
Two, pharmacodynamics screening test
Anti-fatigue test
Trial drug:
Test 1 group: Cordyceps sinensis polysaccharide 0.5g, taurine 2g, 3g;
Test 2 groups: Cordyceps sinensis polysaccharide 0.1g, 0.5g and taurine 0.5g;
3 groups of medicines: Cordyceps sinensis polysaccharide 2g, taurine 10g;
4 groups of medicines: Cordyceps sinensis polysaccharide 1g, taurine 1g;
5 groups of medicines: Cordyceps sinensis polysaccharide 0.8g, taurine 5g;
6 groups of medicines: Cordyceps sinensis polysaccharide 0.6g, LBP-X 4g;
7 groups of medicines: hairworm grass polysaccharide 0.4g, taurine 3g, LBP-X 1g;
Experimental animal: animal 2-3 monthly age, Kunming kind male and healthy mouse, body weight (25 ± 3) g.
Test method: the animal used as test mouse is divided into group, i.e. different pharmaceutical group and control group, every group of 12 mouse are adopted administration by gavage, and give mouse stomach different pharmaceutical with the 0.20g/kg dosage every day, and control group gives equivalent distilled water.1 time/d, continuous 28d.Carried out the mensuration of each item antifatigue index on the 29th day.
Motion model control group and experimental group began to carry out the adaptability swimming instruction on the 21st day in irritating stomach; Swimming time is begun by 10min/d; Increased progressively 5min to the every day 20 days; Power exhausts criterion does not float for the 8s that sinks under water by mouse body weight 5% swimming with a load attached to the body to mouse, can't accomplish righting reflex after pulling out.Power exhausts swimming time: after last was subjected to reagent 30min to mouse, adopting load mode was that a quality is the lead of mouse body weight 5% in the mouse tail, and mouse is placed the swimming case of depth of water 30cm, and the temperature of water is controlled at about 30 ℃.Forced swimming to power exhausts (till the 8s that sinks under water is motionless), the record swimming time.The mensuration of serum urea nitrogen: the 1. preparation of serum: after last is subjected to reagent 30min to mouse; With the swimming case that mouse does not bear a heavy burden and places depth of water 30cm, the temperature of water is controlled at about 30 ℃, swimming 90min; Afterbody blood sampling at once behind the rest 30min, about 0.5ml that takes a blood sample (not adding anti-coagulants).Put 4 ℃ of about 3h of refrigerator, the centrifugal 15min of 2500r/min after the blood clotting gets serum and measures the serum urea nitrogen value.2. determination of urea nitrogen method: press document [comparison [J] of Wei Xu east .3 kind serum urea nitrogen determination method. Chongqing medical science .1997,26 (4): 246.] method measures.The mensuration of hepatic glycogen: the 1. extraction of hepatic glycogen: after last is subjected to reagent 30min to mouse, takes off neck at once and put to death, get liver, inhale the blood of attachment removal rapidly with filter paper.Take by weighing about 1g, put in the mortar, add and clean fine sand a little and 10% trichloroacetic acid 1m, l grinds.Add 5% trichloroacetic acid 2m again, l continues to grind, fully worn into the meat gruel shape to liver organization till.Then with the centrifugal 10min of 2500r/min.Supernatant is changed over to another centrifuge tube and measures volume, add 95% ethanol, after the mixing, leave standstill 10min with volume.This moment, glycogen became flocculent deposit to separate out.Precipitation solution is with the centrifugal 10min of 2500r/min.Abandoning supernatant, and centrifuge tube is inverted in 2min on the filter paper.Add distilled water 1m in the deposition, l stirs with glass bar and is precipitated to dissolving.2. the mensuration of hepatic glycogen: press literature method anthrone-concentrated sulfuric acid method and measure hepatic glycogen content, every group is repeated 3 times, averages.
Table 1 power exhausts swimming time
Annotate: compare * P＜0.05, * * P＜0.01 with control group; Compare △ P＜0.05 for 1 group with test; Compare #P＜0.05 for 2 groups with test.
Table 2 serum urea nitrogen content
| Group | Serum urea nitrogen |
| C/mmo?l·L-1 | |
| Control group | 14.1±1.2 |
| Test 1 group | 10.9±0.8* |
| Test 2 groups | 10.6±0.9* |
| Test 3 groups | 6.2±0.5**△# |
| Test 4 groups | 6.4±0.4**△# |
| Test 5 groups | 6.7±0.5**△# |
| Test 6 groups | 6.2±0.6**△# |
| Test 7 groups | 5.9±0.4**△# |
Annotate: compare * P＜0.05, * * P＜0.01 with control group; Compare △ P＜0.05 for 1 group with test; Compare #P＜0.05 for 2 groups with test.
Table 3 hepatic glycogen content
Annotate: compare * P＜0.05, * * P＜0.01 with control group; Compare △ P＜0.05 for 1 group with test; Compare #P＜0.05 for 2 groups with test.
Conclusion (of pressure testing): above-mentioned test shows that beverage raw material of the present invention has fine effect to antifatigue.
Embodiment
Embodiment 1
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 0.1g and taurine 0.5g.
Aforementioned pharmaceutical compositions is prepared into beverage.
Embodiment 2
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 2g, taurine 10g.
Embodiment 3
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 1g, taurine 1g.
Embodiment 4
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 0.1g, taurine 0.5g, LBP-X 2g.
Embodiment 5
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 1.9g, LBP-X 10g, and plum juice is an amount of.
Embodiment 6
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 0.9g, taurine 1.2g, LBP-X 2g, and rose, glucose, oligoisomaltose are an amount of.
Embodiment 7
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 1.5g, LBP-X 0.5g, Pandan Leaves juice, and glucose, oligoisomaltose are an amount of.
Embodiment 8
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 2g, LBP-X 10g, and rose and oligoisomaltose are an amount of.
Embodiment 9
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 1g, taurine 1.5g.
Embodiment 10
A kind of beverage with antifatigue effect, beverage raw material are Cordyceps sinensis polysaccharide 0.9g, taurine 0.5g and LBP-X 3g, and rose candy, FOS are an amount of.
Above-mentioned said Cordyceps sinensis polysaccharide can be from the natural cs fructification, extract and or from the ARTIFICIAL CULTURE fructification, extract and or extraction and getting from the mycelium of fermented and cultured and zymotic fluid.
Above-mentioned said Cordyceps sinensis polysaccharide can be commercially available purchase gained, perhaps obtains according to following preparation method:
Embodiment 11
Bacterial classification is: Paecilomyces hepiali chen or Hirsutella hepiali Chen et Shen; Culture medium is: glucose 20g, serpentgrass 30g, milk powder 20g, dusty yeast 20g, magnesium sulfate 0.2g, dipotassium hydrogen phosphate 0.2g; The serpentgrass water extracts the back and filters, and use the distilled water constant volume after in filtrating, adding other component mixing while hot, adjust pH to 5, and 18 ℃ of cultivations 3 days down, the secondary feed supplement is: wolfberry fruit extract; After cultivating end, ferment cordyceps sinensis bacterium liquid is filtered, centrifugal, get supernatant, place 30 ℃ of baths, regulate pH value to 4, add proteolytic enzyme and cellulolytic enzyme respectively and carry out enzymolysis, enzymolysis moves into the enzyme that goes out in 100 ℃ of baths immediately after 80 minutes; In 80 ℃ of water-baths, it is condensed into 1/6 of volume, adds 20% ethanol of 3 times of volumes after the cooling while stirring, then 4 ℃ static 12 hours, centrifugal, concentrate, wash back 60 ℃ of constant temperature dryings with absolute ethyl alcohol, both Cordyceps sinensis polysaccharide.
Embodiment 12
Bacterial classification is: Paecilomyces hepiali chen or Hirsutella hepiali Chen et Shen; Culture medium is: glucose 40g, serpentgrass 100g, milk powder 30g, dusty yeast 50g, magnesium sulfate 5g, dipotassium hydrogen phosphate 5g; The serpentgrass water extracts the back and filters, and use the distilled water constant volume after in filtrating, adding other component mixing while hot, adjust pH to 6.5, and 18 ℃ of cultivations 3 days down, the secondary feed supplement is: glucose and/or structure Qi extract; After cultivating end, ferment cordyceps sinensis bacterium liquid is filtered, centrifugal, get supernatant, place 90 ℃ of baths, regulate pH value to 6, add proteolytic enzyme and cellulolytic enzyme respectively and carry out enzymolysis, enzymolysis moves into the enzyme that goes out in 100 ℃ of baths immediately after 200 minutes; In 80 ℃ of water-baths, it is condensed into 1/3 of volume, adds 95% ethanol of 6 times of volumes after the cooling while stirring, then 8 ℃ static 36 hours, centrifugal, concentrate, wash back 60 ℃ of constant temperature dryings with absolute ethyl alcohol, both Cordyceps sinensis polysaccharide.
Embodiment 13
Bacterial classification is: Paecilomyces hepiali chen or Hirsutella hepiali Chen et Shen; Culture medium is: glucose 30g, serpentgrass 35g, milk powder 25g, dusty yeast 35g, magnesium sulfate 0.5g, dipotassium hydrogen phosphate 0.8g; After the serpentgrass water extracts, use the distilled water constant volume after in filtrating, adding other component mixing while hot, adjust pH to 5.5,20 ℃ of cultivations 5 days down, the secondary feed supplement is: glucose and structure Qi extract; After cultivating end, ferment cordyceps sinensis bacterium liquid is filtered, centrifugal, get supernatant, place 45 ℃ of baths, regulate pH value to 4.5, add proteolytic enzyme and cellulolytic enzyme respectively and carry out enzymolysis, enzymolysis moves into the enzyme that goes out in 100 ℃ of baths immediately after 100 minutes; In 80 ℃ of water-baths, it is condensed into 40% the ethanol that adds 4 times of volumes after 1/4 cooling of volume while stirring, then 5 ℃ static 14 hours, centrifugal, concentrate, wash back 60 ℃ of constant temperature dryings with absolute ethyl alcohol, both Cordyceps sinensis polysaccharide.
Embodiment 14
Bacterial classification is: Paecilomyces hepiali chen or Hirsutella hepiali Chen et Shen; Culture medium is: glucose 35g, serpentgrass 75g, milk powder 25g, dusty yeast 35g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g; After the serpentgrass water extracts, use the distilled water constant volume after in filtrating, adding other component mixing while hot, adjust pH to 6,24 ℃ of cultivations 8 days down, the secondary feed supplement is: glucose; After cultivating end, ferment cordyceps sinensis bacterium liquid is filtered, centrifugal, get supernatant, place 75 ℃ of baths, regulate pH value to 5.5, add proteolytic enzyme and cellulolytic enzyme respectively and carry out enzymolysis, enzymolysis moves into the enzyme that goes out in 100 ℃ of baths immediately after 150 minutes; In 80 ℃ of water-baths, it is condensed into 95% the ethanol that adds 5 times of volumes after 1/4 cooling of volume while stirring, then 6 ℃ static 24 hours, centrifugal, concentrate, wash back 60 ℃ of constant temperature dryings with absolute ethyl alcohol, both Cordyceps sinensis polysaccharide.
Embodiment 15
Bacterial classification is: Paecilomyces hepiali chen or Hirsutella hepiali Chen et Shen; Culture medium is: glucose 30g, serpentgrass 95g, milk powder 26g, dusty yeast 44g, magnesium sulfate 2g, dipotassium hydrogen phosphate 3g; After the serpentgrass water extracts, use the distilled water constant volume after in filtrating, adding other component mixing while hot, adjust pH to 6,20 ℃ of cultivations 8 days down, the secondary feed supplement is: glucose and wolfberry fruit extract; After cultivating end, ferment cordyceps sinensis bacterium liquid is filtered, centrifugal, get supernatant, place 75 ℃ of baths, regulate pH value to 6, add proteolytic enzyme and cellulolytic enzyme respectively and carry out enzymolysis, enzymolysis moves into the enzyme that goes out in 100 ℃ of baths immediately after 95 minutes; In 80 ℃ of water-baths, it is condensed into 1/5 of volume, adds 65% ethanol of 5 times of volumes after the cooling while stirring, then 7 ℃ static 14 hours, centrifugal, concentrate, wash back 60 ℃ of constant temperature dryings with absolute ethyl alcohol, both Cordyceps sinensis polysaccharide.
Claims (7)
1. the beverage with antifatigue effect is characterized in that raw material is Cordyceps sinensis polysaccharide, taurine and/or LBP-X in the beverage, wherein Cordyceps sinensis polysaccharide 0.1-2 weight portion, taurine and/or LBP-X 0.5-10 weight portion.
2. a kind of beverage with antifatigue effect according to claim 1 wherein can also add set-in of plum rains juice in the beverage.
3. a kind of beverage with antifatigue effect according to claim 1 wherein can also add rose in the beverage, glucose and/or oligoisomaltose.
4. a kind of beverage with antifatigue effect according to claim 1 wherein can also add Pandan Leaves juice in the beverage, glucose and/or oligoisomaltose.
5. a kind of beverage with antifatigue effect according to claim 1 wherein can also add strawberry, blueberry, raspberry in the beverage; Rubus parvifolius, American Ginseng or its extract, pseudo-ginseng or its extract; Guarana or its extract, genseng or its extract, lysine; Arginine, citrulling, rhodiola root or its extract; Barrenwort or its extract, n-octacosanol, damiana; Tribulus terrestris or its extract, agate card, one or more of eastern brother Ali.
6. a kind of beverage according to claim 2 with antifatigue effect, wherein plum juice refers to prune juice, dark plum juice is stewed plum juice, carving plum juice, a kind of of plum juice wrapped up in Soviet Union.
7. a kind of beverage according to claim 1 with antifatigue effect, wherein the preparation method of Cordyceps sinensis polysaccharide is: bacterial classification is: Paecilomyces hepiali chen or Hirsutella hepiali Chen et Shen; Culture medium is: glucose 20-40 weight portion, serpentgrass 30-100 weight portion, milk powder 20-30 weight portion, dusty yeast 20-50 weight portion, magnesium sulfate 0.2-5 weight portion, dipotassium hydrogen phosphate 0.2-5 weight portion; The serpentgrass water extracts the back and filters, and use the distilled water constant volume after in filtrating, adding other component mixing while hot, and adjust pH is to 5-6.5, and cultivation is 3-10 days under 18-25 ℃, and the secondary feed supplement is: glucose and/or wolfberry fruit extract; After cultivating end, ferment cordyceps sinensis bacterium liquid is filtered, centrifugal, get supernatant, place 30-90 ℃ of bath, regulate the pH value to 4-6, add proteolytic enzyme and cellulolytic enzyme respectively and carry out enzymolysis, move into the enzyme that goes out in 100 ℃ of baths after enzymolysis 80-200 minute immediately; In 80 ℃ of water-baths, it is condensed into the 1/6-1/3 of volume, adds the ethanol of the 20%-95% of 3-6 times of volume after the cooling while stirring, then at 4-8 ℃ of static 12-36 hour; Centrifugal; Concentrate, wash back 60 ℃ of constant temperature dryings, both got Cordyceps sinensis polysaccharide with absolute ethyl alcohol.
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Application publication date: 20120104 |