CN102286604B - Microbiological method for screening dimethoate residues in food - Google Patents
Microbiological method for screening dimethoate residues in food Download PDFInfo
- Publication number
- CN102286604B CN102286604B CN 201110188037 CN201110188037A CN102286604B CN 102286604 B CN102286604 B CN 102286604B CN 201110188037 CN201110188037 CN 201110188037 CN 201110188037 A CN201110188037 A CN 201110188037A CN 102286604 B CN102286604 B CN 102286604B
- Authority
- CN
- China
- Prior art keywords
- screening
- ljm
- food
- bifidobacterium adolescentis
- dimethoate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a bifidobacterium LJM-001 strain. The collection number of the bifidobacterium LJM-001 strain is CGMCC No.4090. The LJM-001 strain is hypersensitive to dimethoate and is a working strain for detecting dimethoate residues in food. The invention also provides a microbiological method for screening dimethoate residues in food. According to the size of antibacterial rings on a screening culture medium, whether the residual quantity of the dimethoate in food samples exceeds the standard or not can be judged. The method for screening the dimethoate residues in food has the characteristics of simpleness and convenience for operation, low cost, high sensitivity and suitability for sample screening, and the screening requirement of a large number of samples in the pesticide residue detection department in China is met.
Description
Technical field
The present invention relates to the application of a strain bifidobacterium adolescentis and the Determination of Dimethoate Residues screening in food of conduct work bacterial strain thereof.
Background technology
Rogor is a kind of efficient moderately toxic organophosphorus pesticide, chemistry O by name, O-dimethyl-S-(N-methylamino formyl methyl) phosphorodithioate (C
6H
12NO
3PS
2), because of its instant effect, the advantage such as cheap, be widely used in desinsection, the sterilization of the farm crop such as vegetable and fruit in China and killed mite.
But the excessive use of Rogor makes its high residue in vegetable and fruit, brings huge threat for people's health, so China's use of having limited the quantity of.In January, 2005 Ministry of Health of the People's Republic of China and Standardization Administration of China issue GB2763-2005 is defined in maximum residue limit (the maximum residue limits of leaf vegetables, MRL) be 1mg/kg, pomaceous fruits fruit MRL is 1mg/kg, stone fruit MRL is 2mg/kg, citrus fruit MRL is 2mg/kg, and will detect in food the Determination of Dimethoate Residues amount and classify residual monitoring emphasis as.
At present, the method for mensuration Rogor mainly contains vapor-phase chromatography (GC), high performance liquid chromatography (HPLC), chemoluminescence method, enzyme inhibition method, capillary electrophoresis technique (CE) etc.Detect with aforesaid method and have plant and instrument complexity, Sample pretreatment and the loaded down with trivial details defective of measurement operation, be not suitable for the great amount of samples screening, and costly, it is promoted the use of be restricted.
The microbial method outstanding advantages is easy and economical, does not need complicated plant and instrument, just can carry out under general experiment condition, can carry out quick primary dcreening operation to great amount of samples.But the work bacterial strain is just the most critical issue that affects microbial method accuracy and precision on susceptibility and the specificity of test substance.Up to now, there is no and filter out high to dimethoate pesticide susceptibility, that specificity is high bifidus bacillus work bacterial strain and the Determination of Dimethoate Residues screening is used in food relevant report thereof.
Summary of the invention
The object of the invention is to provide the strain bifidus bacillus high to Rogor susceptibility.
Bifidus bacillus of the present invention is: bifidobacterium adolescentis (Bifidobacterium adolescentis) LJM-001CGMCC No.4090.
Bifidobacterium adolescentis of the present invention (Bifidobacterium adolescentis) LJM-001, on August 17th, 2010 at China Committee for Culture Collection of Microorganisms's common micro-organisms center, it is referred to as CGMCC (address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101) preservation, Classification And Nomenclature is bifidobacterium adolescentis (Bifidobacterium adolescentis), and deposit number is CGMCC No.4090.
Bifidobacterium adolescentis LJM-001 of the present invention is located away from Zhejiang Province's Healthy Youth human faecal mass.
Bifidobacterium adolescentis LJM-001 of the present invention has following microbial characteristic:
(1) colonial morphology: be faint yellow or oyster white, central uplift, neat in edge, circle, smooth, diameter 1～2mm size.
(2) individual morphology: be irregular G+ sporeless bacterium, it is shaft-like that thalline is, and slightly crooked, what have is V-shaped, is on a small quantity the Y type, and two ends color depth.
(3) physiological and biochemical property: D-ribose (+); L-arabinose (+); Lactose (+); Cellobiose (+); Melizitose (+); Raffinose (+); Sorbyl alcohol (+); Starch (+); Glucose (+); Wood sugar (+); Seminose (+); Fructose (+); Semi-lactosi (+); Maltose (+); Trehalose (+); Melibiose (+); N.F,USP MANNITOL (+); Saligenin (+); Nitrate reduction (-); Catalase (-); Arginine hydrolysis experiment (-); Gelatine liquefication reaction test (-); Indole test (-); F6PPK enzyme (+).
Well-grown under anaerobism is not grown in aerobic environment.25～28 ℃ of 37～41 ℃ of minimum growth temperatures of optimum growth temperature; The highest 43～45 ℃; Growth optimal pH 6.5～7.0; Do not grow in pH4.5～5.0 or 8.0～8.5.
Bifidobacterium adolescentis LJM-001 of the present invention with belong to reference culture of the same race together and compare Rogor susceptibility and improved more than 100 times.
Another object of the present invention is to set up a kind of bifidobacterium adolescentis LJM-001 that uses as the microbial method of work bacterial strain screening food Determination of Dimethoate Residues.
The principle of screening food Determination of Dimethoate Residues of the present invention is based on Rogor its responsive bifidobacterium adolescentis LJM-001 is had obvious restraining effect, on food samples point sample bifidus bacillus bacterium layer screening substratum, anaerobism was cultivated after 18-24 hour, measure the inhibition zone of its formation, can judge according to the size of inhibition zone in food samples to be measured, whether the Determination of Dimethoate Residues amount is qualified.
The present invention utilizes bifidobacterium adolescentis LJM-001 as the microbial method of work bacterial strain screening food Determination of Dimethoate Residues, comprises step:
1, screening substratum preparation: peptone 10g, extractum carnis 10g, yeast extract paste 5g, glucose 20g, sodium acetate 5g, K
2HPO
42g, MgSO
47H
2O 0.5g, MnSO
44H
2O 0.2g, oligofructose 3g, dibasic ammonium citrate 2g, tween-80 1ml, agar 15g, developer 0.1g, distilled water 1L proofreaies and correct pH6.5 with above composition heating for dissolving, 115 ℃ autoclaving 15-20 minute.
Developer is 5-bromo-4-chloro-3-indoles-β-D galactoside (X-Gal).
2, collecting cells: bifidobacterium adolescentis LJM-001 is inoculated in the slant medium of nutrient agar medium, 35 ± 2 ℃ of anaerobism were cultivated 24 hours, with sterile saline, lawn is washed, make bacteria suspension, under 721 spectrophotometer 580nm wavelength, light transmission rate is controlled to be 0.8, puts in 2-8 ℃ of refrigerator and preserve.
3, the dull and stereotyped preparation of bacterium layer: get the flat board of diameter 90mm, high 16～17mm, the screening substratum 20mL that injection heating melts makes and evenly spread out cloth at the bottom of dish, places to make on horizontal stand and solidifies, as bottom; Add bacteria suspension 0.5mL on the bottom substratum that has solidified (letting cool to 48～50 ℃), fully mixing, make and evenly spread out cloth on bottom, as the bacterium layer.
4, sample pre-treatments: get food samples homogenate to be checked and organize 5g, with aseptic 1% phosphate buffered saline buffer (pH6.0) 25ml dilution, the whirlpool mixing is in 80 ℃ of heating in water bath 5min, in the centrifugal 15min of 5000r/min, get supernatant liquor as test sample liquid after cooling.
5, sample determination: (diameter 13 ± 1mm) with the light paper-pressing sheet of tweezers, makes the scraps of paper and substratum close contact, drips test sample liquid 90 μ l on the circle filter paper with micropipet to place the circle filter paper in the culture dish of bacterium layer flat board.Each repeats 2 culture dish at least for sample, and 35 ± 2 ℃ of anaerobism were cultivated 18～24 hours, and antibacterial circle diameter is measured by inhibition zone computer analyzer.
6, result is judged:
As inhibition zone 〉=15mm, Determination of Dimethoate Residues positive (+) in the judgement food samples.
Work bacterial strain of the present invention is bifidobacterium adolescentis LJM-001, on 08 17th, 2010 in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, deposit number is CGMCCNo.4090.
Food samples of the present invention comprises the vegetable and fruits such as Chinese cabbage, eggplant, cucumber, pumpkin, wax gourd, apple, pear, peach.
Advantage of the present invention and effect:
(1) bifidobacterium adolescentis LJM-001 of the present invention is high to Rogor susceptibility, specificity good, can be used as the work bacterial classification of Determination of Dimethoate Residues in microbial process screening food;
(2) added the X-Gal indicator in screening substratum of the present invention, made the inhibition zone of generation more clear;
(3) sample pre-treatments of the present invention is simple, a large amount of food samples of screening simultaneously;
(4) detection method of the present invention is simple and easy to do, have highly sensitive, the tolerance range high, lowest detection is limited to 0.1mg/kg in vegetables, lower than the maximum residue limit of national standard, the rate of recovery is all greater than 70%, and the variation coefficient is in 10%, the accuracy and the precision requirement that meet the screening of food Determination of Dimethoate Residues are fit to the screening of Determination of Dimethoate Residues in food fully.
Embodiment
The invention will be further described below in conjunction with specific embodiment, but illustrated embodiment is not as a limitation of the invention.If do not specialize, the conventional means that in embodiment, technique means used is well known to those skilled in the art.
Separation and the evaluation of embodiment 1 bifidobacterium adolescentis LJM-001
with the ight soil of Zhejiang Province's healthy young people as sample separation, on the modified MRS nutrient agar, under 37 ℃ of anaerobic conditions, 48h is coated with separation and Culture, obtain bifidobacterium adolescentis LJM-001 of the present invention, be accredited as bifidobacterium adolescentis, in on 08 17th, 2010 in (address: No. 3, A, DaTun Road, Chaoyang District, BeiJing City, China Committee for Culture Collection of Microorganisms's common micro-organisms center, Institute of Microorganism, Academia Sinica, postcode 100101) preservation, Classification And Nomenclature is bifidobacterium adolescentis (Bifidobacterium adolescentis), preserving number is CGMCC No.4090.
The formula of modified MRS nutrient agar: peptone 10g, extractum carnis 10g, yeast extract paste 5g, glucose 20g, sodium acetate 5g, K
2HPO
42g, MgSO
47 H
2O 0.5g, MnSO
44H
2O 0.2g, oligofructose 3g, dibasic ammonium citrate 2g, tween-80 1ml, agar 15g, 5-bromo-4-chloro-3-indoles-β-D galactoside (X-Gal) 0.1g, distilled water 1L transfers pH to 7.0, sterilizes 15 minutes for 115 ℃.
Bifidobacterium adolescentis LJM-001 of the present invention has following microbial characteristic:
(1) colonial morphology: be faint yellow or oyster white, central uplift, neat in edge, circle, smooth, diameter 1～2mm size.
(2) individual morphology: be irregular G
+Sporeless bacterium, thalline are shaft-like, and slightly crooked, what have is V-shaped, is on a small quantity the Y type, and two ends color depth.
(3) physiological and biochemical property: D-ribose (+); L-arabinose (+); Lactose (+); Cellobiose (+); Melizitose (+); Raffinose (+); Sorbyl alcohol (+); Starch (+); Glucose (+); Wood sugar (+); Seminose (+); Fructose (+); Semi-lactosi (+); Maltose (+); Trehalose (+); Melibiose (+); N.F,USP MANNITOL (+); Saligenin (+); Nitrate reduction (-); Catalase (-); Arginine hydrolysis experiment (-); Gelatine liquefication reaction test (-); Indole test (-); F6PPK enzyme (+).
Well-grown under anaerobism is not grown in aerobic environment.37～41 ℃ of optimum growth temperatures; 25～28 ℃ of minimum growth temperatures; The highest 43～45 ℃; Growth optimal pH 6.5～7.0; Do not grow in pH4.5～5.0 or 8.0～8.5.
Test 1 sensitivity test
Adopt scraps of paper agar diffusion (K-B) method, choose the Rogor reference liquid of different concns, with the modified MRS nutrient agar, culture medium prescription is seen embodiment 1, take bifidobacterium adolescentis LJM-001 of the present invention as the test bacterium, be placed in 37 ℃ of incubator anaerobism and cultivate the growing state of observing the LJM-001 bacterial strain after 24h, do the Rogor sensitivity test with agar diffusion method of the paper, measure Rogor to the bacteriostasis property of LJM-001 bacterial strain.
3 repetitions are done in above-mentioned test, to belong to bifidobacterium adolescentis reference culture of the same race together as contrast.
Table 1 sensitivity test
Test-results: bifidobacterium adolescentis LJM-001 of the present invention has the susceptibility of height to Rogor, its with belong to reference culture together and compare, susceptibility to Rogor has improved more than 100 times, Rogor susceptibility≤0.1mg/kg of bifidobacterium adolescentis LJM-001, lower than the maximum residue limit (MRL) of national standard, can be used as the work bacterial strain of screening Determination of Dimethoate Residues fully.
Test 2 specific tests
Drip respectively Rogor in alternate Oxford cup with reference to working standard liquid such as concentration (0.1mg/kg) reference liquid and Rogor, acephatemet, acephate, omethoate, SD-1750, thiophos on flat board.Compare bifidobacterium adolescentis LJM-001 of the present invention to the susceptibility of Rogor, acephatemet, acephate, omethoate, SD-1750,6 kinds of agricultural chemicals of thiophos.
Calculate the cross reacting rate of Rogor and other 5 kinds of agricultural chemicals with following formula.
Test-results: bifidobacterium adolescentis LJM-001 of the present invention is the most responsive to Rogor, and is insensitive to other 5 kinds of agricultural chemicals, and in other 5 kinds of agricultural chemicals, the LJM-001 bacterial strain is all insensitive to each agricultural chemicals of 2 times of MRL and following dosage level thereof.
The screening of Determination of Dimethoate Residues in embodiment 2 cabbages leaves
1, screening substratum preparation:
Peptone 10g, extractum carnis 10g, yeast extract paste 5g, glucose 20g, sodium acetate 5g, K
2HPO
42g, MgSO
47H
2O 0.5g, MnSO
44H
2O 0.2g, oligofructose 3g, dibasic ammonium citrate 2g, tween-80 1ml, agar 15g, developer 0.1g, distilled water 1L proofreaies and correct pH6.5 with above composition heating for dissolving, 115 ℃ autoclaving 15-20 minute.
Developer is 5-bromo-4-chloro-3-indoles-β-D galactoside (X-Gal).
2, collecting cells:
Bifidobacterium adolescentis LJM-001 is inoculated in the slant medium of nutrient agar medium, 35 ± 2 ℃ of anaerobism were cultivated 24 hours, with sterile saline, lawn were washed, and made suspension, under 721 spectrophotometer 580nm wavelength, light transmission rate is controlled to be 0.8, puts in 2～8 ℃ of refrigerators and preserve.
3, the dull and stereotyped preparation of bacterium layer:
Get the flat board of diameter 90mm, high 16～17mm, (3) screening substratum 20mL that injection heating melts makes and evenly spread out cloth at the bottom of dish, places to make on horizontal stand and solidifies, as bottom; Add bacteria suspension 0.5mL on the bottom substratum that has solidified (letting cool to 48～50 ℃), fully mixing, make and evenly spread out cloth on bottom, as the bacterium layer.
4, sample pre-treatments:
Get cabbages leaves homogenate to be checked and organize 5g, cooling rear in the centrifugal 15min of 5000r/min in 80 ℃ of heating in water bath 5min with aseptic 1% phosphate buffered saline buffer (pH6.0) 25ml dilution, whirlpool mixing, get supernatant liquor as test sample liquid.
5, sample determination:
(diameter 13 ± 1mm) with the light paper-pressing sheet of tweezers, makes the scraps of paper and substratum close contact, drips test sample liquid 90 μ l on the circle filter paper with micropipet to place the circle filter paper in the culture dish of bacterium layer flat board.Each repeats 2 culture dish at least for sample, and anaerobism is cultivated 35 ± 2 ℃, and 18～24 hours, antibacterial circle diameter was measured by inhibition zone computer analyzer.
6, result is judged:
As inhibition zone 〉=15mm, Determination of Dimethoate Residues positive (+) in the judgement food samples.
Test the mensuration of 1 standard substance precision
Getting concentration is 0.1mg/kg, the Rogor reference liquid of 1mg/kg, pipette 90 μ l with pipettor respectively and add bacterium layer screening media surface, four round filter papers that place on bacterium layer surface are (on diameter 13 ± 1mm), each concentration drips a round filter paper, repeat 5 culture dish, 35 ± 2 ℃ of anaerobism were cultivated 18～24 hours.
The mensuration of table 2 standard substance precision
Annotate: circle filter paper diameter is 13 ± 1mm, the not long bacterium of circle filter paper inferior segment
Test-results: the variation coefficient of 0.1mg/kg Rogor reference liquid is that the variation coefficient of 2.6%, 1mg/kg Rogor reference liquid is 2.5%.The variation coefficient all in 10%, meets the precision requirement of agricultural chemicals screening fully.
Test the mensuration of 2 food samples precision
Get the blank homogenate cabbages leaves of agricultural chemicals 5g, in triplicate, make that in food, Rogor concentration is respectively 0.1mg/L, 1mg/L, pipetting 90 μ l with pipettor adds on four round filter papers that bacterium layer media surface place, each concentration drips 2 round filter papers, repeats 3 culture dish, cultivates 18～24 hours for 35 ± 2 ℃.
Table 3 food samples precision test
Test-results: the variation coefficient between 0.1mg/kg concentration sample plate is 1.0%, and total variation coefficient is 1.31%; Between the dish of 1mg/kg concentration sample, the variation coefficient is 1.56%, and total variation coefficient is 1.85%.
The inventive method variation coefficient all in 10%, meets the precision requirement of agricultural chemicals screening fully.
Test 3 food samples accuracy tests
Get each 5g of the blank homogenate food samples of agricultural chemicals, in quintuplicate, make in food that Rogor concentration is respectively 0.1,0.5,0.75,1,1.25mg/kg, pipetting 90 μ l with pipettor adds on four round filter papers that bacterium layer media surface place, each concentration drips 2 round filter papers, and another 3 splash into reference liquid with reference to concentration.Repeat 5 culture dish, put in the incubator of 35 ± 2 ℃ 18～24 hours, measure antibacterial circle diameter.
Calculate the rate of recovery of Rogor in food samples with following formula.
Table 4 food samples accuracy test
Test-results: in food samples, the Rogor interpolation rate of recovery is 84%～98.4%, in the daytime the variation coefficient is 1.22%～8.79%, the rate of recovery is all greater than 70%, the variation coefficient is in 10%, show that the present invention has higher accuracy and repeatability, meet the requirement of the existing food pesticide residue screening of China fully.
Test 4 false negative rate tests
Take blank each 5g of food samples of agricultural chemicals of homogeneous, add the Rogor reference liquid, make Rogor concentration 1mg/kg in food, measuring method is with embodiment 2, and replication 100 times is observed the probability that negative findings occurs, and calculates false negative rate.
Test-results: when in cucumber, Determination of Dimethoate Residues was 1mg/kg, false negative rate was 0; When in wax gourd, Determination of Dimethoate Residues was 1mg/kg, false negative rate was 2%.
The test of table 5 false negative rate
Claims (3)
1. the application of a strain bifidobacterium adolescentis (Bifidobacterium adolescentis) LJM-001 is characterized in that the screening that a strain bifidobacterium adolescentis LJM-001CGMCC No.4090 is applied to the food Determination of Dimethoate Residues that is applied as of described bacterial strain.
2. the application of a strain bifidobacterium adolescentis LJM-001 according to claim 1 is characterized in that: a described strain bifidobacterium adolescentis LJM-001 is applied to the screening of food Determination of Dimethoate Residues, comprises step:
(1) screening substratum preparation: peptone 10g, extractum carnis 10g, yeast extract paste 5g, glucose 20g, sodium acetate 5g, K
2HPO
42g, MgSO
4.7H
2O0.5g, MnSO
44H
2O0.2g, oligofructose 3g, dibasic ammonium citrate 2g, tween-80 1ml, agar 15g, developer 5-bromo-4-chloro-3-indoles-β-D galactoside (X-Gal) 0.1g, distilled water 1L, heating for dissolving is proofreaied and correct pH6.5,115 ℃ autoclaving 15-20 minute;
(2) collecting cells: bifidobacterium adolescentis LJM-001 is inoculated in the slant medium of nutrient agar medium, and 35 ± 2 ℃ of anaerobism were cultivated 24 hours, with sterile saline, lawn were washed, and made bacteria suspension, put in 2-8 ℃ of refrigerator and preserved;
(3) the dull and stereotyped preparation of bacterium layer: get the flat board of diameter 90mm, high 16～17mm, the screening substratum 20mL that injection heating melts makes and evenly spread out cloth at the bottom of dish, places to make on horizontal stand and solidifies, as bottom; Let cool to 48～50 ℃, add bacteria suspension 0.5mL on the bottom substratum that has solidified, fully mixing, make and evenly spread out cloth on bottom, as the bacterium layer;
(4) sample pre-treatments: get food samples homogenate to be checked and organize 5g, with the sterile phosphate damping fluid 25ml dilution of 1%pH6.0, the whirlpool mixing is in 80 ℃ of heating in water bath 5min, in the centrifugal 15min of 5000r/min, get supernatant liquor as test sample liquid after cooling;
(5) sample determination: place the round filter paper of diameter 13 ± 1mm in the culture dish of bacterium layer flat board, with the light paper-pressing sheet of tweezers, make the scraps of paper and substratum close contact, drip test sample liquid 90 μ 1 with micropipet on the circle filter paper; Each repeats 2 culture dish at least for sample, and 35 ± 2 ℃ of anaerobism were cultivated 18～24 hours, and antibacterial circle diameter is measured by inhibition zone computer analyzer;
(6) result is judged: when inhibition zone 〉=15mm, and Determination of Dimethoate Residues positive (+) in the judgement food samples.
3. the application of a strain bifidobacterium adolescentis LJM-001 according to claim 2, it is used for the screening of food Determination of Dimethoate Residues, it is characterized in that, wherein the described bacteria suspension of step (2) light transmission rate under 721 spectrophotometer 580nm wavelength is controlled to be 0.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110188037 CN102286604B (en) | 2011-06-25 | 2011-06-25 | Microbiological method for screening dimethoate residues in food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110188037 CN102286604B (en) | 2011-06-25 | 2011-06-25 | Microbiological method for screening dimethoate residues in food |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102286604A CN102286604A (en) | 2011-12-21 |
| CN102286604B true CN102286604B (en) | 2013-06-05 |
Family
ID=45333358
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110188037 Expired - Fee Related CN102286604B (en) | 2011-06-25 | 2011-06-25 | Microbiological method for screening dimethoate residues in food |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102286604B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789267B (en) * | 2014-02-21 | 2016-05-11 | 刘洛贤 | A kind of improved primary hippocampal neurons method |
| CN106222235B (en) * | 2016-07-13 | 2020-02-18 | 温州医科大学 | Method for rapidly screening and evaluating pesticide residues in tea |
| CN106282295B (en) * | 2016-07-13 | 2020-06-23 | 温州医科大学 | Method for rapidly screening tea pesticide residues based on microbial toxicity |
| CN109490490A (en) * | 2018-07-02 | 2019-03-19 | 马鞍山清净环保科技有限公司 | The remaining detection method of thimet in a kind of vegetables |
| CN109971670B (en) * | 2019-02-01 | 2023-01-24 | 温州医科大学 | Enterococcus faecalis, culture medium thereof, and method for detecting antibiotic residues in milk and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1888059A (en) * | 2006-07-13 | 2007-01-03 | 上海交通大学 | Recombinat acetylcholinesterase and its prepn process and usage in detecting presticide residue |
| CN101241113A (en) * | 2007-02-07 | 2008-08-13 | 劲牌有限公司 | Chinese herbal medicine residual organophosphorus pesticide detection method |
| CN101333257A (en) * | 2008-08-07 | 2008-12-31 | 江苏省农业科学院 | Broadspectrum specificity polyclone antibody of methoxyl organophosphorus pesticide and uses thereof |
| CN101343325A (en) * | 2008-08-21 | 2009-01-14 | 上海交通大学 | Antibody preparation method capable of detecting multiple organophosphorus pesticide residuals |
-
2011
- 2011-06-25 CN CN 201110188037 patent/CN102286604B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1888059A (en) * | 2006-07-13 | 2007-01-03 | 上海交通大学 | Recombinat acetylcholinesterase and its prepn process and usage in detecting presticide residue |
| CN101241113A (en) * | 2007-02-07 | 2008-08-13 | 劲牌有限公司 | Chinese herbal medicine residual organophosphorus pesticide detection method |
| CN101333257A (en) * | 2008-08-07 | 2008-12-31 | 江苏省农业科学院 | Broadspectrum specificity polyclone antibody of methoxyl organophosphorus pesticide and uses thereof |
| CN101343325A (en) * | 2008-08-21 | 2009-01-14 | 上海交通大学 | Antibody preparation method capable of detecting multiple organophosphorus pesticide residuals |
Non-Patent Citations (4)
| Title |
|---|
| 微量果汁中痕量乐果的快速质谱检测;王姜 等;《分析化学》;20100430;第38卷(第4期);453-457 * |
| 王姜 等.微量果汁中痕量乐果的快速质谱检测.《分析化学》.2010,第38卷(第4期),453-457. |
| 细菌发光传感器对污染物急性毒性快速检测的研究;闫鹏 等;《中国卫生工程学》;20021231;第1卷(第2期);65-68 * |
| 闫鹏 等.细菌发光传感器对污染物急性毒性快速检测的研究.《中国卫生工程学》.2002,第1卷(第2期),65-68. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102286604A (en) | 2011-12-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102286604B (en) | Microbiological method for screening dimethoate residues in food | |
| Pedersen et al. | Nitrogen fixation (acetylene reduction) associated with roots of winter wheat and sorghum in Nebraska | |
| CN102485880B (en) | Bacillus amyloliquefaciens and application thereof | |
| Lin et al. | Changes of soil microbiological properties caused by land use changing from rice–wheat rotation to vegetable cultivation | |
| CN102268471B (en) | Microbiological method for detecting acephate residues in food | |
| Trevors et al. | Electron transport system activity in soil, sediment, and pure cultures | |
| Shila et al. | Detection of Pseudomonas syringae pv. lachrymans associated with the seeds of cucurbits | |
| CN102304481B (en) | Bifidobacterium adolescentis strain | |
| Adetunji et al. | Production of phytotoxic metabolites with bioherbicidal activities from Lasiodiplodia pseudotheobromae produced on different agricultural wastes using solid-state fermentation | |
| CN102168053B (en) | Paenibacilluspolymyxa strain for coproduction of LI-F type antibiotic and dibutyl phthalate and application thereof | |
| CN102590196B (en) | The detection method of antibacterial medicine residue in a kind of Rapid Screening animal foodstuff sample | |
| CN102304482B (en) | Cifidobacterium longum strain | |
| CN102517230B (en) | Bifidobacterium breve and detection method of methamidopho pesticide residue in foodstuff | |
| CN105400717A (en) | Bacterial strain HBRM-16 capable of promoting growth of roots of rubber tree and application of bacterial strain HBRM-16 | |
| Gabriel-Ajobiewe et al. | Basal media formulation using Canavalia ensiformis as carbon and nitrogen source for the growth of some fungi species | |
| CN106282295B (en) | Method for rapidly screening tea pesticide residues based on microbial toxicity | |
| CN103333834B (en) | Bacterium for degrading herbicide imazethapyr and application of bacterium | |
| CN106222235B (en) | Method for rapidly screening and evaluating pesticide residues in tea | |
| CN102329734B (en) | Aspergillus fumigatus and application thereof to degradation of imazethapyr | |
| Rasmann et al. | Resilient populations of root fungi occur within five tomato production systems in southeast Florida | |
| KR20030031371A (en) | Bacillus subtilis YS1 having antifungal activity, preparation method of its mutants by gamma radiation and the mutants thereof | |
| CN106929444B (en) | Bacillus and application thereof | |
| Abdullahi et al. | Isolation and identification of bacteria associated with aerial part of rice plant from Kware lake | |
| CN106085872B (en) | A kind of mixing endogenetic fungus that can promote acacia confusa Nutrient Absorption | |
| Balabel | Detection of Ralstonia solanacearum phylotype II, sequevar 1 in seasonal weed plants associated with potato cultivations in Egypt |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Jiaming Inventor after: Fang Fang Inventor after: Shi Hongying Inventor after: Zhong Chongzhou Inventor after: Wang Wenwei Inventor after: Lu Zhongqiu Inventor after: Hong Guangliang Inventor before: Liu Jiaming Inventor before: Du Jimei Inventor before: Yao Wei Inventor before: Lin Gang |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: LIU JIAMING DU JIMEI YAO WEI LIN GANG TO: LIU JIAMING FANG FANG SHI HONGYING ZHONG CHONGZHOU WANG WENWEI LU ZHONGQIU HONG GUANGLIANG |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: WENZHOU MEDICAL UNIVERSITY RENJI COLLEGE Free format text: FORMER OWNER: WENZHOU MEDICINAL INSTITUTE Effective date: 20140520 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 325035 WENZHOU, ZHEJIANG PROVINCE TO: 325000 WENZHOU, ZHEJIANG PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20140520 Address after: 325000 Zhejiang province Chashan Wenzhou Higher Education Park Patentee after: Shanghai Institute of Wenzhou Medical University Address before: 325035 Zhejiang province Chashan Wenzhou Higher Education Park of Wenzhou Medical College Patentee before: Wenzhou Medical College |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130605 Termination date: 20180625 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |