CN102283109B - Breeding method of new variety of banana - Google Patents
Breeding method of new variety of banana Download PDFInfo
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- CN102283109B CN102283109B CN 201010202124 CN201010202124A CN102283109B CN 102283109 B CN102283109 B CN 102283109B CN 201010202124 CN201010202124 CN 201010202124 CN 201010202124 A CN201010202124 A CN 201010202124A CN 102283109 B CN102283109 B CN 102283109B
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Abstract
The invention relates to a breeding method of a new variety of banana, which comprises the five steps of sterile material acquisition, bud differentiation and proliferation, adventitious bud strong stock cultivation, rooting cultivation, and seedling adaptation and transplantation to obtain banana sprouts. Compared with the prior art, through a tissue culture technique, the breeding speed and the neatness of nursery stock can be greatly increased, the original female parent properties can be better maintained, the survival rate can be improved, thus, the output is increased, and the transplanting survival rate can reach 95%.
Description
Technical field
The present invention relates to a kind of herbal tissue culture method, especially relate to a kind of method of cultivation of new variety of banana.
Background technology
' Rowe Red ' plant is large-scale draft to banana, and is high about 1 meter, and red streak is arranged on the leaf, and sight is peculiar, belongs to small-sized plant in banana belongs to, and is to see the vegetable material that leaf is the master.Banana mainly in the torrid areas cultivation, is also paid attention at Temperate Region in China at present very much.As the Introduced Cultivars comparatively small amt of external introduction, its plant division is slow, but fixing market demand is arranged, and the huge market demand, and the seedling supply is restricted.By tissue culture technique, greatly improve the reguarity of reproduction speed and seedling, keep better original maternal character.' the group training report of Rowe Red ' there is not yet banana.
Summary of the invention
Purpose of the present invention is exactly to provide a kind of reguarity that can greatly improve reproduction speed and seedling for the defective that overcomes above-mentioned prior art existence, keeps better the method for cultivation of a kind of new variety of banana of original maternal character.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of method of cultivation of new variety of banana is characterized in that, the method is acquisition, bud part Differentiation and proliferation, indefinite bud strong seedling culture, root culture and hardening and the transplanting five-step approach that comprises sterilizable material, and concrete steps are as follows:
(1) acquisition of sterilizable material: the budlet that takes the banana base portion, with behind the tap water flushing 2h on Bechtop, be the alcohol immersion 20-40s of 75wt% with concentration, the mercuric chloride solution of 1wt ‰ soaks 10-20min, behind aseptic water washing 5-6 time, blot surperficial moisture content with aseptic filter paper, get the budlet base portion and be cut into that 1cm is long to be inoculated on the inducing culture;
(2) Differentiation and proliferation of bud is: budlet was inoculated on the inducing culture after 3 weeks, the budlet base portion begins to expand, and the yellow-green colour projection occurs, visible significantly callus after 1 week, cultivated 1 month, and then downcut band bud callus and put into the adventitious bud proliferation substratum and cultivate;
(3) indefinite bud strong seedling culture
The Multiple Buds that in the adventitious bud proliferation substratum, induces, the Da Cong bud is divided into Xiao Cong or simple bud after, put on the strong seedling culture base, indefinite bud extends rapidly, can grow up to the plantlet of 2-3cm after 20 days;
(4) root culture
Get and highly be the plantlet of 2-3cm, switching enters root induction in the root media, and the seedling base section dissolves the former base of root of many whites after 10 days, can grow to 4-6cm after 30 days;
(5) hardening and transplanting
Root culture 20-30 days, when root system grows to 1-2cm, select the aseptic seedling of well developed root system robust growth, indoor uncork hardening 3 days is taken out afterwash root agar, and domestication after 40 days in the greenhouse can be transplanted outdoorly, gives rich water quality management and gets final product.
The composition of described budlet inducing culture is MS+6-BA1.0mg/L+NAA0.1mg/L, MS+6-BA2.0mg/L+NAA0.2mg/L or MS+6-BA3.0mg/L+NAA0.3mg/L.
The preferred MS+6-BA2.0mg/L+NAA0.2mg/L of the composition of described budlet inducing culture.
The composition of described proliferated culture medium is MS+6-BA1.0mg/L+NAA0.1mg/L, MS+6-BA2.0mg/L+NAA0.2mg/L or MS+6-BA3.0mg/L+NAA0.3mg/L.
The preferred MS+6-BA1.0mg/L+NAA0.1mg/L of the composition of described proliferated culture medium.
The composition of described strong seedling culture base is MS+6-BA0.5mg/L+NAA0.1mg/L, MS+6-BA1.0mg/L+NAA0.1mg/L or MS+6-BA2.0mg/L+NAA0.2mg/L.
The preferred MS+6-BA0.5mg/L+NAA0.1mg/L of the composition of described strong seedling culture base.
The composition of described root media is MS+NAA0.05mg/L, MS+NAA0.1mg/L or MS+NAA0.2mg/L.
The preferred MS+NAA0.1mg/L of the composition of described root media.
Described budlet inducing culture, proliferated culture medium, strong seedling culture base and root culture based component also comprise sucrose 30g/L, agar 6g/L, and the culture temperature of this substratum is 24-26 ℃, and illumination is 70-90 μ mol/ms, and the pH value is 5.5-6.0.
Compared with prior art, the present invention can improve the reguarity of reproduction speed and nursery stock greatly by tissue culture technique, keeps better original maternal character, and can improve surviving rate, thereby improve output.
Embodiment
The present invention is described in detail below in conjunction with specific embodiment.
Embodiment 1
A kind of method of cultivation of new variety of banana, the method may further comprise the steps:
(1) asepticize is processed
Take the budlet of banana base portion, with behind the tap water flushing 2h on Bechtop, be the alcohol immersion 30s of 75wt% with concentration, the mercuric chloride solution of 1wt ‰ soaks 15min, behind aseptic water washing 5-6 time, blot surperficial moisture content with aseptic filter paper, get the budlet base portion and be cut into that 1cm is long to be inoculated on the budlet inducing culture MS+6-BA2.0mg/L+NAA0.2mg/L;
(2) Differentiation and proliferation of bud
Budlet was inoculated on the budlet inducing culture after 3 weeks, the budlet base portion begins to expand, the yellow-green colour projection appears, visible significantly callus was cultivated after 1 month after 1 week, and cutting-out band bud callus is put into proliferated culture medium MS+6-BA1.0mg/L+NAA0.1mg/L and cultivated, the base portion callus is many, but do not affect the propagation of indefinite bud, the indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, and it is good to grow in this cultivation;
(3) indefinite bud strong seedling culture
Induce Multiple Buds at proliferated culture medium, every clump of Multiple Buds only has the 2-3 strain to extend, and all the other are in the dwarfing state, after the Da Cong bud is divided into Xiao Cong or simple bud, put on the strong seedling culture base MS+6-BA0.5mg/L+NAA0.1mg/L, indefinite bud extends rapidly, can grow 2-3cm after 20 days;
(4) root culture
Get and highly be the plantlet of 2-3cm, switching enters root induction among the root media MS+NAA0.1mg/L, and the seedling base section dissolves the former base of root of many whites after 10 days, can grow to 4-6cm after 30 days, and root system is sturdy, and fibrous root is numerous, and rooting rate is 100%;
(5) hardening and transplanting
Root culture 25 days when root system grows to 1-2cm, is selected the aseptic seedling of well developed root system robust growth, indoor uncork hardening 3 days is taken out afterwash root agar, and domestication is after 40 days in the greenhouse, can transplant outdoorly, give rich water quality management and get final product, transplanting survival rate is 95%.
Embodiment 2
A kind of method of cultivation of new variety of banana, the method may further comprise the steps:
(1) asepticize is processed
Take the budlet of banana base portion, with behind the tap water flushing 2h on Bechtop, be the alcohol immersion 20s of 75wt% with concentration, the mercuric chloride solution of 1wt ‰ soaks 10min, behind aseptic water washing 5-6 time, blot surperficial moisture content with aseptic filter paper, get the budlet base portion and be cut into that 1cm is long to be inoculated on the budlet inducing culture MS+6-BA1.0mg/L+NAA0.1mg/L;
(2) Differentiation and proliferation of bud
Budlet was inoculated on the budlet inducing culture after 3 weeks, the budlet base portion begins to expand, the yellow-green colour projection appears, visible significantly callus was cultivated after 1 month after 1 week, and cutting-out band bud callus is put into proliferated culture medium MS+6-BA2.0mg/L+NAA0.2mg/L and cultivated, the base portion callus is many, but do not affect the propagation of indefinite bud, the indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, and it is good to grow in this cultivation;
(3) indefinite bud strong seedling culture
Induce Multiple Buds at proliferated culture medium, every clump of Multiple Buds only has the 2-3 strain to extend, and all the other are in the dwarfing state, after the Da Cong bud is divided into Xiao Cong or simple bud, put on the strong seedling culture base MS+6-BA1.0mg/L+NAA0.1mg/L, indefinite bud extends rapidly, can grow 2-3cm after 20 days;
(4) root culture
Get and highly be the plantlet of 2-3cm, switching enters root induction among the root media MS+NAA0.05mg/L, and the seedling base section dissolves the former base of root of many whites after 10 days, can grow to 2-3cm after 30 days;
(5) hardening and transplanting
Continue root culture 20 days, and when root system grows to 1-2cm, selected the aseptic seedling of well developed root system robust growth, indoor uncork hardening 3 days is taken out afterwash root agar, and domestication is after 40 days in the greenhouse, can transplant outdoorly, give rich water quality management and get final product, transplanting survival rate is 95%.
Embodiment 3
A kind of method of cultivation of new variety of banana, the method may further comprise the steps:
(1) asepticize is processed
Take the budlet of banana base portion, with behind the tap water flushing 2h on Bechtop, be the alcohol immersion 40s of 75wt% with concentration, the mercuric chloride solution of 1wt ‰ soaks 20min, behind aseptic water washing 5-6 time, blot surperficial moisture content with aseptic filter paper, get the budlet base portion and be cut into that 1cm is long to be inoculated on the budlet inducing culture MS+6-BA3.0mg/L+NAA0.3mg/L;
(2) Differentiation and proliferation of bud
Budlet was inoculated on the budlet inducing culture after 3 weeks, the budlet base portion begins to expand, the yellow-green colour projection appears, visible significantly callus was cultivated after 1 month after 1 week, and cutting-out band bud callus is put into proliferated culture medium MS+6-BA3.0mg/L+NAA0.3mg/L and cultivated, the base portion callus is many, but do not affect the propagation of indefinite bud, the indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, and it is good to grow in this cultivation;
(3) indefinite bud strong seedling culture
Induce Multiple Buds at proliferated culture medium, every clump of Multiple Buds only has the 2-3 strain to extend, and all the other are in the dwarfing state, after the Da Cong bud is divided into Xiao Cong or simple bud, put on the strong seedling culture base MS+6-BA2.0mg/L+NAA0.2mg/L, indefinite bud extends rapidly, can grow 2-3cm after 20 days;
(4) root culture
Get and highly be the plantlet of 2-3cm, switching enters root induction among the root media MS+NAA0.2mg/L, and the seedling base section dissolves the former base of root of many whites after 10 days, can grow to 2-3cm after 30 days;
(5) hardening and transplanting
Continue root culture 30 days, and when root system grows to 1-2cm, selected the aseptic seedling of well developed root system robust growth, indoor uncork hardening 3 days is taken out afterwash root agar, and domestication is after 40 days in the greenhouse, can transplant outdoorly, give rich water quality management and get final product, transplanting survival rate is 95%.
Embodiment 4
A kind of method of cultivation of new variety of banana, the method may further comprise the steps:
(1) asepticize is processed
Take the budlet of banana base portion, with behind the tap water flushing 2h on Bechtop, be the alcohol immersion 30s of 75wt% with concentration, the mercuric chloride solution of 1wt ‰ soaks 15min, behind aseptic water washing 5-6 time, blot surperficial moisture content with aseptic filter paper, get the budlet base portion and be cut into that 1cm is long to be inoculated on the budlet inducing culture MS+6-BA3.0mg/L+NAA0.3mg/L;
(2) Differentiation and proliferation of bud
Budlet was inoculated on the budlet inducing culture after 3 weeks, the budlet base portion begins to expand, the yellow-green colour projection appears, visible significantly callus was cultivated after 1 month after 1 week, and cutting-out band bud callus is put into proliferated culture medium MS+6-BA1.0mg/L+NAA0.1mg/L and cultivated, the base portion callus is many, but do not affect the propagation of indefinite bud, the indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, and it is good to grow in this cultivation;
(3) indefinite bud strong seedling culture
Induce Multiple Buds at proliferated culture medium, every clump of Multiple Buds only has the 2-3 strain to extend, and all the other are in the dwarfing state, after the Da Cong bud is divided into Xiao Cong or simple bud, put on the strong seedling culture base MS+6-BA0.5mg/L+NAA0.1mg/L, indefinite bud extends rapidly, can grow 2-3cm after 20 days;
(4) root culture
Get and highly be the plantlet of 2-3cm, switching enters root induction among the root media MS+NAA0.1mg/L, and the seedling base section dissolves the former base of root of many whites after 10 days, can grow to 2-3cm after 30 days, and root system is sturdy, and fibrous root is numerous, and rooting rate is 100%;
(5) hardening and transplanting
Continue root culture 25 days, and when root system grows to 1-2cm, selected the aseptic seedling of well developed root system robust growth, indoor uncork hardening 3 days is taken out afterwash root agar, and domestication is after 40 days in the greenhouse, can transplant outdoorly, give rich water quality management and get final product, transplanting survival rate is 95%.
Described budlet inducing culture, proliferated culture medium, strong seedling culture base and root culture based component also comprise sucrose 30g/L, agar 6g/L, and the culture temperature of this substratum is 24 ℃, and illumination is 70 μ mol/ms, and the pH value is 5.5.
Embodiment 5
A kind of method of cultivation of new variety of banana, the method may further comprise the steps:
(1) asepticize is processed
Take the budlet of banana base portion, with behind the tap water flushing 2h on Bechtop, be the alcohol immersion 20s of 75wt% with concentration, the mercuric chloride solution of 1wt ‰ soaks 10min, behind aseptic water washing 5-6 time, blot surperficial moisture content with aseptic filter paper, get the budlet base portion and be cut into that 1cm is long to be inoculated on the budlet inducing culture MS+6-BA1.0mg/L+NAA0.1mg/L;
(2) Differentiation and proliferation of bud
Budlet was inoculated on the budlet inducing culture after 3 weeks, the budlet base portion begins to expand, the yellow-green colour projection appears, visible significantly callus was cultivated after 1 month after 1 week, and cutting-out band bud callus is put into proliferated culture medium MS+6-BA2.0mg/L+NAA0.2mg/L and cultivated, the base portion callus is many, but do not affect the propagation of indefinite bud, the indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, and it is good to grow in this cultivation;
(3) indefinite bud strong seedling culture
Induce Multiple Buds at proliferated culture medium, every clump of Multiple Buds only has the 2-3 strain to extend, and all the other are in the dwarfing state, after the Da Cong bud is divided into Xiao Cong or simple bud, put on the strong seedling culture base MS+6-BA1.0mg/L+NAA0.1mg/L, indefinite bud extends rapidly, can grow 2-3cm after 20 days;
(4) root culture
Get and highly be the plantlet of 2-3cm, switching enters root induction among the root media MS+NAA0.05mg/L, and the seedling base section dissolves the former base of root of many whites after 10 days, can grow to 2-3cm after 30 days;
(5) hardening and transplanting
Continue root culture 20 days, and when root system grows to 1-2cm, selected the aseptic seedling of well developed root system robust growth, indoor uncork hardening 3 days is taken out afterwash root agar, and domestication is after 40 days in the greenhouse, can transplant outdoorly, give rich water quality management and get final product, transplanting survival rate is 95%.
Described budlet inducing culture, proliferated culture medium, strong seedling culture base and root culture based component also comprise sucrose 30g/L, agar 6g/L, and the culture temperature of this substratum is 25 ℃, and illumination is 80 μ mol/ms, and the pH value is 5.8.
Embodiment 6
A kind of method of cultivation of new variety of banana, the method may further comprise the steps:
(1) asepticize is processed
Take the budlet of banana base portion, with behind the tap water flushing 2h on Bechtop, be the alcohol immersion 40s of 75wt% with concentration, the mercuric chloride solution of 1wt ‰ soaks 20min, behind aseptic water washing 5-6 time, blot surperficial moisture content with aseptic filter paper, get the budlet base portion and be cut into that 1cm is long to be inoculated on the budlet inducing culture MS+6-BA2.0mg/L+NAA0.2mg/L;
(2) Differentiation and proliferation of bud
Budlet was inoculated on the budlet inducing culture after 3 weeks, the budlet base portion begins to expand, the yellow-green colour projection appears, visible significantly callus was cultivated after 1 month after 1 week, and cutting-out band bud callus is put into proliferated culture medium MS+6-BA3.0mg/L+NAA0.3mg/L and cultivated, the base portion callus is many, but do not affect the propagation of indefinite bud, the indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, and it is good to grow in this cultivation;
(3) indefinite bud strong seedling culture
Induce Multiple Buds at proliferated culture medium, every clump of Multiple Buds only has the 2-3 strain to extend, and all the other are in the dwarfing state, after the Da Cong bud is divided into Xiao Cong or simple bud, put on the strong seedling culture base MS+6-BA2.0mg/L+NAA0.2mg/L, indefinite bud extends rapidly, can grow 2-3cm after 20 days;
(4) root culture
Get and highly be the plantlet of 2-3cm, switching enters root induction among the root media MS+NAA0.2mg/L, and the seedling base section dissolves the former base of root of many whites after 10 days, can grow to 2-3cm after 30 days;
(5) hardening and transplanting
Continue root culture 30 days, and when root system grows to 1-2cm, selected the aseptic seedling of well developed root system robust growth, indoor uncork hardening 3 days is taken out afterwash root agar, and domestication is after 40 days in the greenhouse, can transplant outdoorly, give rich water quality management and get final product, transplanting survival rate is 95%.
Described budlet inducing culture, proliferated culture medium, strong seedling culture base and root culture based component also comprise sucrose 30g/L, agar 6g/L, and the culture temperature of this substratum is 26 ℃, and illumination is 90 μ mol/ms, and the pH value is 6.0.
Claims (1)
1. one kind is used for the substratum that Banana Tissue is cultivated, and it is characterized in that comprise budlet inducing culture, proliferated culture medium, strong seedling culture base and root media, wherein, the composition of described budlet inducing culture is MS+6-BA1.0mg/L+NAA0.1mg/L; The composition of described proliferated culture medium is MS+6-BA2.0mg/L+NAA0.2mg/L; The composition of described strong seedling culture base is MS+6-BA1.0 mg/L+NAA0.1mg/L; The composition of described root media is MS+NAA0.05mg/L.
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102613053B (en) * | 2012-04-05 | 2013-05-01 | 中国热带农业科学院环境与植物保护研究所 | Water culture method for banana tissue-culture seedlings |
| CN103444535A (en) * | 2013-09-05 | 2013-12-18 | 广东省农业科学院果树研究所 | Novel method for increasing tissue culture and rapid propagation coefficients of banana |
| CN104871975A (en) * | 2015-05-27 | 2015-09-02 | 杨树东 | Banana tissue culture propagation method |
| CN106718947A (en) * | 2017-03-22 | 2017-05-31 | 黄庆辉 | One kind promotes the value-added tissue culture method of banana callus |
| CN106942055A (en) * | 2017-03-22 | 2017-07-14 | 黄庆辉 | A kind of chaff adenine phosphate banana proliferated culture medium screening technique |
| CN106942054A (en) * | 2017-03-22 | 2017-07-14 | 黄庆辉 | A kind of banana increment culture medium with chaff adenine phosphate |
| CN107027629A (en) * | 2017-05-04 | 2017-08-11 | 北海康维生态农业科技有限公司 | A kind of method for tissue culture for improving banana survival rate |
| CN107232055A (en) * | 2017-06-14 | 2017-10-10 | 中国热带农业科学院海口实验站 | A Xi any of several broadleaf plants breeding methods based on improved culture medium |
| CN110692517A (en) * | 2019-10-10 | 2020-01-17 | 高州市石生源生物科技发展有限公司 | Banana tissue culture breeding method |
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2010
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