CN102277228A - Preparation method and application of refined ostrich oil - Google Patents
Preparation method and application of refined ostrich oil Download PDFInfo
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- 238000001914 filtration Methods 0.000 claims abstract description 8
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- Cosmetics (AREA)
Abstract
The invention belongs to the technical field of chemical invention, particularly relating to a preparation method and application of refined ostrich oil. The preparation method of the ostrich oil comprises the following steps: taking ostrich fat tissue as a raw material, mincing, putting into an extraction device, adding water, pressurizing to extract, and filtering and separating to obtain crude ostrich oil; melting the crude ostrich oil, adding dilute aqueous alkali, stirring, standing for layering, and collecting an oil phase; carrying out centrifugal dehydration after an oil layer is melted; collecting an upper layer of clarified oil phase; and drying in vacuum to remove trace moisture so as to obtain the refined ostrich oil. The ostrich oil disclosed by the invention can be used for preparing cosmetics, medical paste and body surface inflammation resistant drug. The preparation method has the advantages of simple technology, small equipment investment and high yield, is convenient to operate, does not contain organic solvent and is environmentally-friendly.
Description
Technical field
The invention belongs to chemical invention technical field, be specifically related to a kind of preparation method and its usage of refining ostrich oil.
Background technology
African Ostrich (Struthio camelus) is the maximum in the present birds, and it may be evolved by the ancestors of circling in the air, but itself can not fly, is African local animal, mainly is distributed in Sub-Sahara.All in the world now African Ostrich all belong to same race, can be divided into 4 existing subspecies and the subspecies that destroied the evidence 1 modern age in the biology classification.
Wild ostrich belongs to " CITS " protection category, now development and use for raising and train ostrich.Raise and train ostrich and be meant that through the production population of artificial hybridization seed selection one-tenth can develop, as circulation of commodities, its breed, exploitation, utilization start from South Africa.The nineties in 20th century, China begins to introduce the cultural technique of ostrich, carries out industrialization development, and is developed rapidly.China has become Asia first at present, the fifth-largest ostrich in the world is cultured state.Its product mainly comprises ostrich meat, skin, egg and feather etc., is the important source of luxury food, famous and precious leather, artwork, medicine and finishing material.
Ostrich oil derives from the fat of ostrich; show after deliberation; mainly form by lipid acid such as oleic acid, palmitinic acid, Zoomeric acid, linolic acid and stearic acid; its unique lipid acid is formed; form similar to the lipid acid of human body skin; and do not contain phosphatide in the ostrich oil; so ostrich oil is to the seepage force of human body skin and to take the medicine ability all stronger; in addition; ostrich oil also has and promotes effects such as wound healing, relieving inflammation and relaxing pain and protection be cardiovascular, so ostrich oil has extremely application prospects in makeup and health care industry.
At present, the domestic report of introducing the ostrich oil health keeping and medical function increases gradually.Relate to the preserve moisture making invention of cosmetic cream of a kind of novel sunscreen that contains the ostrich essential oil as: patent CN200610105029.1, it adopts natural matter, has the desalination freckle, gets rid of pigmentation, keeps skin life, prevents the advantage of water loss.Patent CN200610042792.0 then utilizes ostrich oil and middle Tibetan medicine to develop a kind of paste for the treatment of wound and burn and scald.Patent CN200910021991.0 then utilizes ostrich oil to develop a kind of moisturizing and whitening cream agent.
Extracting method about ostrich oil also has relevant patent report, but all be to adopt solvent (ether, sherwood oil, supercritical co) to extract, extraction yield with organic solvent extraction is very high, but there is potential safety hazard, adopt supercritical carbon dioxide extraction to avoid problems such as dissolvent residual, but extraction efficiency but reduce greatly.A kind of extraction of ostrich oil and oil, fat separation method have been reported as: patent CN200910021992.5, adopt ether to do the solvent extraction ostrich oil, liquid oil of centrifugation and solid-state fat then, this method adopts ether as extracting solvent, but ether is inflammable and explosive, and human body is had anesthetic action, in use have great potential safety hazard.Patent CN200910102292.9 has reported a kind of supercritical extraction method of ostrich oil, this method has been avoided problems such as dissolvent residual, but this preparation method's devices needed investment is big, the technical requirements height, production run cost height, and the extraction yield of this method is very low, and extraction yield has only about 40%, causes the waste of raw material.Patent CN201010120721.8 provides a kind of and has extracted the greasy method of ostrich with petroleum ether solvent, and this method makes the extraction yield of ostrich oil can be up to 90%, but the subject matter of this method is dissolvent residual, if misoperation is easily brought negative impact.
Summary of the invention
The extraction and the process for purification that the purpose of this invention is to provide the ostrich oil of a kind of simple, safety, efficient height, economic environmental protection.
The present invention adopts the pressurized water extracting method, makes protein denaturation by heating, thereby the destruction of adipose tissue cell flows out fat, obtains slightly oil of ostrich.
The present invention adopts sig water neutralization to remove free fatty acids in the thick oil of ostrich, be washed till neutrality again, and dehydration obtains refining ostrich oil.
The present invention is achieved through the following technical solutions:
(1) be raw material with the ostrich fatty tissue, rub into meat slurry after, in the extraction element of packing into, every gram raw material adds the water of 5～20mL, extracts 1～2 time in 95～130 ℃, extraction time 1～3h will extract gained oil-water mixture filtered through gauze afterwards, the filtering dregs of fat; Then, oil-water mixture is placed under 40～60 ℃ of environment, standing demix, aqueous phase discarded obtains slightly oil of ostrich.
(2) the gained ostrich is thick oily in 50～80 ℃ of thawings, add 1～10% dilute alkaline soln, the ratio of oil and alkali lye is 1: 0.5～1: 2, stirs 10～30min under 50～80 ℃ of temperature, places then under 40～60 ℃ of environment, standing demix, discard the buck phase, the hot wash of using 60～80 ℃ again is more than 2 times, and washing is to neutral, aqueous phase discarded is collected oil phase.
(3) with oil phase in 50～80 ℃ of thawings, centrifuge dehydration again, the whizzer temperature is set to 30～60 ℃, rotating speed is 3000～7000rpm, and centrifugation time is 20～60min, after centrifugal the finishing, collect the flaxen clarification oil phase in upper strata, again with oil phase 30～60 ℃ of following vacuum hydro-extractions, obtain the refining ostrich oil of flaxen liquid state, stored refrigerated is flaxen solid-state ostrich oil.
In view of the present situation of ostrich oil extracting method, it is the pressurised extraction ostrich oil and the purified novel method of solvent that the present invention proposes with water.The characteristics of this method are: technology is simple, easy to operate, facility investment is little, not with an organic solvent, environmental protection, and the extraction yield of ostrich oil is improved.
Through pharmaceutical research, show that the refining ostrich oil that obtains has that perviousness is strong, effects such as relieving inflammation and relaxing pain and promotion wound healing, therefore refining ostrich oil can be used for the amendment of makeup and the auxiliary material of body surface inflammation and medical paste.
Embodiment
Be described further by the extraction and the process for purification of specific examples ostrich oil disclosed in this invention.
Embodiment 1
After the ostrich fatty tissue cleaned, rub with mincer, it is standby to place refrigerator and cooled to freeze.Take by weighing 230g ostrich fatty tissue, place in the vertical high-pressure steam sterilizing pan, add 1500mL water, it is 95 ℃ that temperature is set, and extraction time is 1h, and freezing air is got rid of in heating earlier, when treating that a large amount of white vapor are discharged, close vent valve, when treating that temperature reaches 95 ℃, self-clocking behind the extraction 1h, is treated its automatic cooling, when tensimeter is shown as 0, open vent valve earlier, open pot cover again, take out extract, use filtered through gauze, the filtering dregs of fat, oil-water mixture is poured in the separating flask, place in 40 ℃ the baking oven standing demix, aqueous phase discarded, divide oil-yielding stratum, obtain slightly oil of 114g ostrich, refrigeration is placed as faint yellow solid, and yield is 49.56%.
Embodiment 2
Take by weighing 200g ostrich fatty tissue, place in the vertical high-pressure steam sterilizing pan, add 1500mL water, it is 105 ℃ that temperature is set, and extraction time is 1h, and freezing air is got rid of in heating earlier, when treating that a large amount of white vapor are discharged, close vent valve, when treating that temperature reaches 105 ℃, self-clocking behind the extraction 1h, is treated its automatic cooling, when tensimeter is shown as 0, open vent valve earlier, open pot cover again, take out extract, use filtered through gauze, the filtering dregs of fat, oil-water mixture is poured in the separating flask, place in 50 ℃ the baking oven standing demix, aqueous phase discarded, divide oil-yielding stratum, obtain slightly oil of 128g ostrich, refrigeration is placed as faint yellow solid, and yield is 64.00%.
Embodiment 3
Take by weighing 210g ostrich fatty tissue, place in the vertical high-pressure steam sterilizing pan, add 1000mL water, it is 115 ℃ that temperature is set, and extraction time is 1h, and freezing air is got rid of in heating earlier, when treating that a large amount of white vapor are discharged, close vent valve, when treating that temperature reaches 115 ℃, self-clocking behind the extraction 1h, is treated its automatic cooling, when tensimeter is shown as 0, open vent valve earlier, open pot cover again, take out extract, use filtered through gauze, the filtering dregs of fat, oil-water mixture is poured in the separating flask, place in 60 ℃ the baking oven standing demix, aqueous phase discarded, divide oil-yielding stratum, obtain slightly oil of 138g ostrich, refrigeration is placed as faint yellow solid, and yield is 65.71%.
Embodiment 4
Take by weighing 228g ostrich fatty tissue, place in the vertical high-pressure steam sterilizing pan, add 1000mL water, it is 125 ℃ that temperature is set, and extraction time is 1h, and freezing air is got rid of in heating earlier, when treating that a large amount of white vapor are discharged, close vent valve, when treating that temperature reaches 125 ℃, self-clocking behind the extraction 1h, is treated its automatic cooling, when tensimeter is shown as 0, open vent valve earlier, open pot cover again, take out extract, use filtered through gauze, the filtering dregs of fat, oil-water mixture is poured in the separating flask, place in 60 ℃ the baking oven standing demix, aqueous phase discarded, divide oil-yielding stratum, obtain slightly oil of 144g ostrich, refrigeration is placed as faint yellow solid, and yield is 63.16%.
Embodiment 5
Getting the thick oil of ostrich that embodiment 1-4 obtained makes with extra care.Get slightly oil of 128g ostrich,, add 10% aqueous sodium hydroxide solution, heated and stirred 30min in 40 ℃ of heating and melting, then oil-water mixture is poured in the separating flask, placed in 40 ℃ the baking oven standing demix, aqueous phase discarded, oil reservoir are used 80 ℃ hot wash 3 times again, and detecting pH value be neutrality.With oily temperature rise to 80 ℃, divide water with whizzer then, the whizzer temperature is made as 30 ℃, and rotating speed is 3000rpm, centrifugal 20min, then with oil 60 ℃ of following vacuum hydro-extractions, must make with extra care ostrich oil 76g, yield is 59.38%.
Embodiment 6
Get slightly oil of 154g ostrich,, add 2% aqueous sodium hydroxide solution, heated and stirred 30min in 50 ℃ of heating and melting, then oil-water mixture is poured in the separating flask, placed in 50 ℃ the baking oven standing demix, aqueous phase discarded, oil reservoir are used 80 ℃ hot wash 3 times again, and detecting pH value be neutrality.With oily temperature rise to 80 ℃, divide water with whizzer then, the whizzer temperature is made as 40 ℃, and rotating speed is 5000rpm, centrifugal 40min, then with oil 40 ℃ of following vacuum hydro-extractions, must make with extra care ostrich oil 114g, yield is 74.03%.
Embodiment 7
Get slightly oil of 144g ostrich,, add 1% aqueous sodium hydroxide solution, heated and stirred 30min in 60 ℃ of heating and melting, then oil-water mixture is poured in the separating flask, placed in 60 ℃ the baking oven standing demix, aqueous phase discarded, oil reservoir are used 80 ℃ hot wash 3 times again, and detecting pH value be neutrality.With oily temperature rise to 80 ℃, divide water with whizzer then, the whizzer temperature is made as 60 ℃, and rotating speed is 7000rpm, centrifugal 60min, then with oil 60 ℃ of following vacuum hydro-extractions, must make with extra care ostrich oil 112g, yield is 77.78%.
Embodiment 8
According to national standard peroxide value, acid number, iodine number (GB/T 5532-2008), saponification value (GB/T5534-2008), specific refractory power (NC-02263), proportion (SN/T 0801.8-1999), moisture and volatile matter content content (GB/T9696-2008) and the fusing point (GB/T 24892-2010) of refining ostrich oil are measured; And use GC-FID that the fatty acid component of ostrich oil has been carried out qualitative and quantitative detection.
The physical and chemical index of the refining ostrich oil that embodiment 5-7 makes the results are shown in Table 1, and fatty acid component GC-FID analytical results sees Table 2:
The physical and chemical index of the refining ostrich oil of table 1
The fatty acid component analysis (GC-FID) (content %) of the refining ostrich oil of table 2
| The lipid acid kind | Example 5 | Example 6 | Example 7 |
| Tetradecanoic acid (C14:0) | 1.00 | 0.74 | 1.07 |
| Tetradecane monoenoic acid (C14:1n5) | 0.13 | 0.08 | 0.13 |
| Pentadecylic acid (C15:0) | 0.22 | 0.14 | 0.19 |
| Palmitinic acid (C16:0) | 33.79 | 30.66 | 30.28 |
| Zoomeric acid (C16:1) | 8.96 | 8.98 | 9.64 |
| Margaric acid (C17:0) | 0.34 | 0.21 | 0.32 |
| Heptadecane monoenoic acid (C17:1) | 0.16 | 0.16 | 0.17 |
| Oleic acid (C18:1) | 35.70 | 38.53 | 33.20 |
| Antiform oleic acid (C18:1n9t) | 0.39 | 0.27 | 0.16 |
| Linolic acid (C18:2) | 10.37 | 11.78 | 15.89 |
| Stearic acid (C18:0) | 6.68 | 5.89 | 5.21 |
| Linolenic acid (C18:3n6) | 1.24 | 1.72 | 2.67 |
| Linolenic acid (C18:3n3) | 0.34 | 0.37 | 0.37 |
| Other | 0.68 | 0.47 | 0.7 |
| Add up to | 100 | 100 | 100 |
Embodiment 9
This patent is inquired into the influence of the guinea pig skin inflammation that ostrich oil causes trichoroacetic acid(TCA), respectively with fat of Oromaius norvaehollandeae and positive contrast of lanolin and negative control.
Experimental technique: by the skin allergic reaction correct of stipulating in safety evaluation of cosmetics program and the method, cavy was bought at this laboratory observation after 1 week, selected healthy animal, was divided into 3 groups at random, removed back wool with electric razor, was about 4 * 6cm
2, get the undamaged animal of skin complete behind the 24h for examination, 6 of every concentration groups, male and female half and half.Being tried thing is trichoroacetic acid(TCA), and trichoroacetic acid(TCA) is coated with skin, removes to be tried to observe behind the thing to be subjected to examination district's skin erythema, oedema and reaction such as fester, and carries out in pairs to afterbody from the head, and top is A, B; C, D; E, F be totally 3 pairs of 6 points.Each dot spacing 1.2cm, each is to spacing 2.5cm.Order was observed respectively in the 1st day, 2 days, 3 days, 4 days, 5 days, 8 days～the 18th day successively.
Experimental result sees Table 3:
Table 3 trichoroacetic acid(TCA) is to the guinea pig skin sensitization test
Annotate: 0: no pathology; 1,2,3,4 represent lesion degree respectively.
Conclusion: the lanolin group does not have influence in the 1st day animal, is the inflammation peak time of animal more than the 2nd day, and this inflammation lasted till the 18th day always, except that the skin inflammation, and began to fester, and lasts till that always experiment finishes.The ostrich oil group also presented obvious inflammation from the 2nd day, this process lasts till always and began in the 8th day to alleviate that inflammation finishes after the 12nd day.The fat of Oromaius norvaehollandeae group had tangible inflammation on the 2nd day, and 3-5 days inflammation begin to descend, and inflammation finishes substantially after the 8th day.In ostrich oil group and the experiment of fat of Oromaius norvaehollandeae group, cavy infection do not occur and waits performance except skin inflammation.This shows that ostrich oil and fat of Oromaius norvaehollandeae have certain effect to skin inflammation.
Embodiment 10
This patent has been inquired into the perviousness of ostrich oil and to the nourishing effect of skin, and with fat of Oromaius norvaehollandeae and lanolin in contrast.
Experimental technique: this test directly is applied in ostrich oil, fat of Oromaius norvaehollandeae and three kinds of samples of lanolin on the human body skin, experiences according to the sense organ of different people, waits as excitement, greasy feeling and marks, relatively the sense organ difference of three kinds of samples.The double-blind method is taked in experiment.Participating in the test number is 28 people, male 14 people, women 14 people, the age all between 20～30 years old, skin matter health.The testee exposes arm, use earlier warm water cleaning, dry, dip in by the tester then and get an amount of sample, be applied in testee's inboard arm respectively equably, require the testee respectively at 10min, 30min and 60min after to the sense organ of three kinds of samples impression keep the score respectively, standards of grading are 1～5 minute, 1 expression is the lightest, and 5 expressions are the heaviest, add up total points at last.
Last scoring statistics is seen Table 4, and as can be seen, the score of ostrich oil and fat of Oromaius norvaehollandeae differs and is not very big on the total points statistics, but ostrich oil and fat of Oromaius norvaehollandeae differ bigger with the score situation of lanolin.With regard to excitement, score value is high more, represents that then the excitement of this sample is big more, and from data statistics as seen, the excitement of lanolin is greater than fat of Oromaius norvaehollandeae and ostrich oil; With regard to absorptivity, score value is high more, represents that then the perviousness of this sample is good more, and from data statistics as seen, the perviousness of fat of Oromaius norvaehollandeae is slightly strong than ostrich oil, and the perviousness of fat of Oromaius norvaehollandeae and ostrich oil is all greater than lanolin.
Table 4 ostrich oil, fat of Oromaius norvaehollandeae and lanolin cutaneous sense investigation statistics
Embodiment 11
This patent has been inquired into the effect of ostrich oil promotion wound healing, and with fat of Oromaius norvaehollandeae, lanolin, paraffin oil in contrast.
Experimental technique:
After SD rat (female) is bought, after one week, select healthy animal, be divided into 5 groups at random, 5 every group, be respectively paraffin group, lanolin group, fat of Oromaius norvaehollandeae group, ostrich oil group and model group at this laboratory observation.Every rat back loses hair or feathers with 8%Na2S, and the depilation region area is about 10cm
2Behind the depilation 24h, weigh, according to 0.3% dosage abdominal injection, the 10% Chloral Hydrate solution of its body weight it is anaesthetized, the zone of behind about 10min the back being lost hair or feathers places 95 ℃ of thermostat water bath 5s, causes the back scalding model, begins administration behind the 24h.Get an amount of paraffin oil, lanolin, fat of Oromaius norvaehollandeae, ostrich oil heating in water bath 10min sterilization, temperature begins to give respectively paraffin group, lanolin group, fat of Oromaius norvaehollandeae group, ostrich oil group SD rat back scald wound district coating after reducing to room temperature, not administration of model group, once a day.Create all soft tissue rednesses, surface of a wound blister swelling degree, wound healing time, wound healing rate and pathology and histological observation after observing topical agent.After observing for three weeks, after SD rat cervical vertebra dislocation execution, get its skin of scalding of fritter, after fixing with 10% formalin solution, make pathological section, observe every group of scalded skin pathologic condition.
The scoring detailed rules and regulations:
5 minutes, the blister swelling of white was scalded the edge and is pink or dark red, or the big area erythema;
4 minutes, the blister of white was flat flat, and the scald place is dark red, and the edge is hard, is yellowish-brown, little the upwarping of forming a scab;
3 minutes, whole dark red spot in scald place and incrustation can be seen newborn skin, after the part incrustation comes off pus were arranged;
2 minutes, incrustation all came off, and is new erythema after scab comes off;
1 minute, skin was almost recovered.
The wound healing rate is calculated:
Describe with scalding the position with transparent film after the SD burned rats, estimate its scalding area with graph paper; After treating that experiment finishes, survey area is calculated the wound healing rate once more.
Conclusion: can find out that from table 5 ostrich oil has the effect of tangible promotion wound healing to the SD burned rats.The calculation result of wound healing rate shows that ostrich oil promotes that the wound healing rate is the highest, and there were significant differences with model group and paraffin group; Fat of Oromaius norvaehollandeae and lanolin promote that the wound healing rate is also higher, with model group significant difference are arranged also.The result of scoring shows that the wound tissue healing of burned rat models of ostrich oil group is the fastest, to the basic healing of the 3rd week; The rat of lanolin group has the sign of obvious deterioration at first all surface of a wound, it is good to be not so good as fat of Oromaius norvaehollandeae group, paraffin group and model group, but resume speed increases after the 10th day, to the 3rd week its healing state slightly better than paraffin group and model group, be inferior to the fat of Oromaius norvaehollandeae group slightly.
Table 5 is respectively organized rat skin healing rate and scoring
★P＜0.01 expression relatively has utmost point significant difference with model group;
◆P＜0.01 expression relatively has utmost point significant difference with paraffin group;
☆Relatively there were significant differences with model group in p＜0.05 expression.
In sum, because ostrich oil has good moistness and perviousness, contain a large amount of unsaturated fatty acidss; skin had special protecting effect; ostrich oil can be used as the makeup amendment, and its main effect makes skin moisturizing, bright and clean for preserving moisture, seal, nourish and lubricating.Ostrich oil can be added in the makeup to the makeup of prescription Pear Power, sun-proof, effect such as preserve moisture with suitable dosage as amendment.Ostrich oil also relieving inflammation and relaxing pain, promote the function of wound healing, so ostrich oil can also be used to promote the treatment of wound healing and various inflammation as the transdermal carrier of various medicines.
Claims (5)
1. the preparation method of a refining ostrich oil is characterized in that, realizes by following steps:
(1) is raw material with the ostrich fatty tissue, rubs, place extraction element, add entry, pressurised extraction 1～2 time, each 1～2 hour extraction time, extracting temperature is 95～125 ℃, with extracting liquid filtering, the filtering dregs of fat with in the oil-water mixture impouring separating flask, place standing demix under 40～60 ℃ of environment again, aqueous phase discarded obtains slightly oil of ostrich;
(2) with the thick oil of ostrich in 40～80 ℃ of thawings, add 1～10% dilute alkaline soln, stir, in and the free fatty acids in the thick oil of ostrich, place standing demix under 40～60 ℃ of environment then, aqueous phase discarded, and with hot wash to neutral, collect oil phase;
(3) with centrifuge dehydration after 40～80 ℃ of thawings of oil reservoir, the whizzer temperature is set to 30～60 ℃, rotating speed is 3000～7000rpm, centrifugation time is 20～60min, behind centrifugal the finishing, moisture micro-in the oil phase is removed in collection upper strata clarification oil phase and 30～60 ℃ of vacuum-dryings, obtains making with extra care ostrich oil at last.
2. the preparation method of a kind of refining ostrich oil according to claim 1 is characterized in that, extracting temperature described in the step (1) is 105～125 ℃, and separation temperature is 50～60 ℃.
3. the preparation method of a kind of refining ostrich oil according to claim 1 is characterized in that, dilute alkaline soln concentration is 1～5% described in the step (2), and separation temperature is 50～60 ℃.
4. the preparation method of a kind of refining ostrich oil according to claim 1, it is characterized in that the whizzer temperature is 40～60 ℃ described in the step (3), rotating speed is 5000～7000rpm, centrifugation time is 30～60min, and the vacuum-drying temperature is 50～60 ℃.
5. the purposes of an ostrich oil is characterized in that: the medicine that is used to make makeup, medical paste and antibody table inflammation.
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| CN103113984A (en) * | 2013-03-04 | 2013-05-22 | 浙江绿升生物科技有限公司 | Improved process for refining mixed oil |
| CN105670779A (en) * | 2016-03-09 | 2016-06-15 | 湛江市博泰生物化工科技实业有限公司 | Method for extracting euphausia superba oil through nitrogen pressurizing water vapor |
| CN105861136A (en) * | 2015-01-22 | 2016-08-17 | 北京世纪旌旗生物科技股份有限公司 | Method for extraction and separation of crocodile oil |
| CN106509659A (en) * | 2016-10-30 | 2017-03-22 | 南宁多灵生物科技有限公司 | Struthio camelus bone sugarless tablets as well as preparation method and application thereof |
| CN107446696A (en) * | 2016-01-27 | 2017-12-08 | 黄楷能 | Poultry grease treatment method |
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| CN113440618A (en) * | 2021-07-06 | 2021-09-28 | 朱景平 | Transdermal matrix and preparation method thereof |
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| CN103113984A (en) * | 2013-03-04 | 2013-05-22 | 浙江绿升生物科技有限公司 | Improved process for refining mixed oil |
| CN105861136A (en) * | 2015-01-22 | 2016-08-17 | 北京世纪旌旗生物科技股份有限公司 | Method for extraction and separation of crocodile oil |
| CN107446696A (en) * | 2016-01-27 | 2017-12-08 | 黄楷能 | Poultry grease treatment method |
| CN105670779A (en) * | 2016-03-09 | 2016-06-15 | 湛江市博泰生物化工科技实业有限公司 | Method for extracting euphausia superba oil through nitrogen pressurizing water vapor |
| CN106509659A (en) * | 2016-10-30 | 2017-03-22 | 南宁多灵生物科技有限公司 | Struthio camelus bone sugarless tablets as well as preparation method and application thereof |
| CN109181883A (en) * | 2018-10-24 | 2019-01-11 | 新疆工程学院 | A kind of production technology of refined horse oil ethyl ester |
| CN109181883B (en) * | 2018-10-24 | 2021-10-29 | 新疆工程学院 | Production process of refined horse oil ethyl ester |
| CN111117749A (en) * | 2019-12-09 | 2020-05-08 | 上海阿道润滑科技有限公司 | High-lubrication low-foam micro-emulsion cutting fluid and preparation method thereof |
| CN113440618A (en) * | 2021-07-06 | 2021-09-28 | 朱景平 | Transdermal matrix and preparation method thereof |
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