CN102273467B - Application of metal derivative containing chlorophyll and hydrolyzate thereof as plant growth regulator - Google Patents
Application of metal derivative containing chlorophyll and hydrolyzate thereof as plant growth regulator Download PDFInfo
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Abstract
The invention provides an application of a metal derivative containing chlorophyll and hydrolyzate thereof as a plant growth regulator. The metal derivative containing chlorophyll and hydrolyzate thereof is selected from chlorophyll metal chelate, a chlorophyllin metal chelate and a monomeric metal chelate obtained by separating a chlorophyllin mixture. The chlorophyll is a green pigment extracted from a natural plant raw material and provided with a phaeophytin mother ring structure. The chlorophyllin is a mixture of multiple dihydroporphin components obtained by hydrolyzing the chlorophyll.The monomeric chlorophyllin component is selected from dihydroporphin-e4, dihydroporphin-e6 or phaeophorbide a monomethyl ester. Metal ions comprise bivalent or trivalent transition metal manganese, iron, copper or zinc ions in an oxidation state. The plant growth regulator provided by the invention has the advantages of safety, non-toxicity, low residue, strong activity, good effect, stable product property, controllable and reliable quality and easiness in promotion and application; the chlorophyll content of the plant can be increased, the root growth is promoted, the germination ratio is increased, the stress resistance is enhanced and the yield is also increased.
Description
Technical field
The present invention relates to a kind of new purposes of material, be specifically related to a kind of metal derivative of chlorophyll and hydrolysate thereof that contains as the application of new plant growth regulator.
Background technology
Chlorophyll is that plant carries out photosynthetic main pigment, extensively is present in the chloroplast of higher plant, and be photosynthetic material base.On the chemical constitution, chlorophyll is the compound of a class chelated mineral magnesium, contain total chlorophyll of about 0.25% in the plant leaf blade, its formation mainly contains multiple isomer such as chlorophyll a, chlorophyll b, Chlorofucsin, CHLOROPHYLL d, the chlorophyll of natural extraction is the mixture of these isomer (monomer), and the common ground of these monomer isomer is to have the female ring of pheophytin (phorbin) structure; Chlorophyll has abundant chemical reactivity, forming chelate with transition metal is the important reaction of a chlorophyllous class, can obtain the product that a class has using value thus is the chlorophyll metal derivative, these products have been widely used in food and cosmetics (A.M.Humphrey, CHLOROPHYLL, Food Chemistry 5 (1980) 57-67).Food toner copper chlorophyll namely is that raw material removes chelated magnesium earlier with chlorophyll, adopts copper (Cu then
+ 2) salt makes with chlorophyll reaction under the organic solvent condition, be easy to prepare derivative (the chlorophyllous synthetic and spectral investigation of transition metal of divalent metal Mn, Zn, Fe, Co, Ni etc. or its corresponding trivalent metal with similar methods, the natural science progress, 1999 (9) 5:405～412; Chang Rubo, Wang Mengliang, the synthetic and spectral investigation of bacteriochlorophyll transient metal complex, University Of Shanxi's journal (natural science edition) 28 (2): 169～172,2005).
The catabolite (general designation chlorophyllin) that the chlorophyll hydrolysis obtains containing carboxyl is the another kind of important reaction that chlorophyll has significant application value.Because chlorophyll is multiple isomer mixture, therefore its hydrolysis gained chlorophyllin product also is the very complicated mixture of composition, studies show that, what obtain according to the chlorophyllous hydrolysis of differential responses condition (as acid-base value, temperature, reaction time, solvent etc.) all is the chlorophyllin product that multiple heterogeneity ratio is mixed, the basic reaction conditions hydrolysis obtains containing the chlorophyllin product of chelated magnesium, and the acid reaction condition, then hydrolysis gets the pheophorbide product.Because cost, commercialization chlorophyllin product adopt chlorophyll basic hydrolysis preparation and get.Each constituent structure difference is that side chain and carboxyl quantity have nothing in common with each other in the chlorophyllin product, but the parent nucleus ring of all have chlorin (chlorin) or pheophytin (phorbin), thereby easily is combined the generation metallo-chelate with metal ion.The same with the complicated ingredient of chlorophyllin, its chlorophyllin metallo-chelate remains the mixture (S.Chernomorsky of multiple heterogeneity, Variability of the composition of chlorophyllin, Mutation Research, and composition is more more complicated than chlorophyll 324 (1994) 177-178).Although each constituent structure of forming all contains same parent nucleus, only there are differences at side chain, but the chlorophyllin of mixed state, its corresponding metallo-chelate of each monomer chlorophyllin composition show bigger difference or have diverse character aspect biologically active, these are all relevant with complexity and the chelate structure of chlorophyllin composition.In addition, the polytropy of metal in the chelating product-sour ratio of components also is the major reason that produces nature difference, this is namely relevant with the preparation condition technical method, also exist certain related with participation chelated metal ionic valence condition and ionic radius, bivalent metal ion is because radius is bigger, tenor not only is difficult to reach 1: 1 mol ratio and content often has than great fluctuation process in its chelate, as copper chlorophyllin sodium salt and sodium iron chlorophyllin salt (food applications) commodity, copper content and the iron content of product quality mark are respectively 4-6% and 〉=2%, the tenor of 1: 1 chelating mol ratio far below 9.2% and 8.2%.
The chlorophyllin metallo-chelate has been widely used in daily chemical industry and food (A.M.Humphrey, CHLOROPHYLL, Food Chemistry 5 (1980) 57-67).Important commercial prod in enormous quantities mainly is copper chlorophyllin sodium salt and the sodium iron chlorophyllin salt (M.Ishidate as the food toner, Jr, T.Sofuni, K.Yoshikawa, M.Hayashi, T.Nohmi, M.Sawada And A.Matsuoka, Primary Mutagenicity Screening Of Food Additives Currently Used In Japan, Fd Chem.Toxic.Vol.22, No.8, pp.623～636,1984), in addition, the chlorophyllin metallo-chelate also is widely used in clinical as medicine.But the metallo-chelate application agriculturally of chlorophyll and chlorophyllin thereof at present is rare, yet there are no bibliographical information as the use research of plant growth regulator.
The chlorophyllin metallo-chelate is prepared under certain condition by chlorophyllin and slaine and gets, and removes the chelated magnesium in the chlorophyllin earlier, is replaced with other metals and makes (Zhang Guangming, Xu Deyu etc., chlorophyll degradation product metal complex synthetic and right
60The radioprotection of Co radiation murine; Acta Pharmaceutica Sinica Acta Pharmaceutica Sinica 1998; 33 (10): 748～754), owing to water-soluble reason, the non-chelating carboxyl of chlorophyllin metallo-chelate is prepared into water-soluble products by the alkalization salify usually and uses with convenient.The existing big quantity research report of its preparation method, synthetic (JP57177064) as food toner Sodium Copper Chlorophyllin, manganese (III) chlorophyllin and iron (III) chlorophyllin (V.V.Borovkov, J.M.Lintuluoto and Y.Inoue, Convenient Method for Efficient Iron and Manganese Ion Insertion into Various Porphyrins under Mild Conditions, Synlett 1999; 1999:61-62), preparation (the Wang Lijuan of the sodium iron chlorophyllin of food and medicinal usage, Qian Xueren, the preparation of leaf of bamboo sodium iron chlorophyllin and property research, chemistry of forest product and industry, 2005 (25) 3:89～92), the chlorophyllin metal derivative zinc chlorophyllin of biologically active (the synthetic and Preliminary Clinical of zinc chlorophyllin a), cobalt (II) chlorophyllin (synthesizing of chlorophyll a cobalt (II)-vitamin PP), chromium (III) chlorophyllin (the synthetic and clinic trial pre-test of chromium chlorophyllin sodium) etc., the iron chlorophyllin sodium salt also can be by the preparation of traditional Chinese medicine silkworm excrement, and its advantage is that price is low, the quality better steady quality.
Chlorophyllin contains multiple isomer composition, is to form complicated mix product.The composition that commercially available copper chlorophyllin sodium salt is indicated contains chlorophyllin diacid copper sodium (chlorin-e4 copper sodium), chlorophyllin three sour copper sodiums (chlorin-e6 copper sodium) respectively, also contains the sodium salt composition of other nearly 10 kinds of chlorin class formation chelated coppers simultaneously.The product of different merchandise resourceses, according to technology and different (the Alan Mortensen of its mixing chlorin components in proportions of raw material, Asger Geppel, HPLC-MS analysis of the green food colorant sodium copper chlorophyllin, Innovative Food Science and Emerging Technologies 8 (2007) 419-425).Chlorination chlorophyllin iron rule is made by chlorophyllin and ferric chloride reaction, mainly contain chlorination chlorophyllin diacid iron (chlorination chlorin-e4 closes iron), composition (Y.Nonomura such as chlorination chlorophyllin three sour iron (chlorination chlorin-e6 closes iron) and chlorination chlorophyllin a mono-methyl iron, Deng, High-performance liquid chromatographic separation of iron (III) chlorophyllin, Journal of Chromatography A, 721 (1996) 350-354), by commercially available these three kinds of monomeric compounds of the separable acquisition of chlorination chlorophyllin iron product, and the product that is commonly referred to chlorination chlorophyllin iron is the multiple constituents mixt that is main component with these three kinds of monomeric compounds.
Have been found that for a long time, transition metal such as zinc, manganese, copper, iron are the necessary micronutrient elements of plant growth, it is the part that promotes various organized enzymes such as photosynthesis, respiration and material transformation or coenzyme in the plant corpus, at the plant corpus intensive amount between 0.2～200mg/kg, modern study shows that many transition elements such as chromium, cobalt, nickel etc. also have adjusting/promotion plant growth effect with the part rare earth element, yet these metallic elements are not also as the application report of plant growth regulator.Transition metals such as zinc, manganese, copper, iron use as trace element fertilizer with mineral salt or simple complex-type compound usually, its actual amount of application reaches every mu 1～3500 gram not to be waited, because product forms is simple, environment for use is complicated and the difficult application process of holding, the long-term use namely easily causes phytotoxicity and may cause the environmental pollution that is difficult to administer.
The application of plant growth regulating substance is plant physiology and one of agrotechnical major progress in modern age, though plant endogenous property growth regulating-activity material (＜1 μ mol/L) under extremely low concentration namely shows strong activity, but its content is atomic, be difficult to extract preparation, thereby price is high, therefore, the endogenic growth regulatory substance of non-plant namely becomes the emphasis of research.Plant growth regulator has natural extraction and artificial synthetic two big classes, at present, the application that puts goods on the market of 100 multiple products is arranged, an important technology method (Fu Hualong etc. that improve and improve the agricultural output quality have been become, the research of plant growth regulator and application, biological processing, 2008 (6) 4:7～12).Plant growth regulator generally has low concentration and promotes and the characteristic of high concentration inhibition plant growth, must use the effect of can bringing into normal play with narrow dosage range, and this causes practical application to have many restrictions and inconvenience; Plant endogenous sex hormone has natural metabolic pathway and mode, be difficult for savings, compare with it, the present exogenous synthetic class plant growth regulator that uses, the using dosage higher concentration is bigger than normal, and metabolic pathway is unclear, chemical property is stable, residual more or the longevity of residure is long, continuous expansion along with range of application, its residue problem has caused people's great attention (Zhao Min etc., plant growth regulator is to crops and environmentAL safety, environment and health magazine, 2007 (24) 5:370～372), China stipulates, any pollution-free food production process, and the form that is not able to fertilizer and agricultural chemicals is used plant growth regulator (the agricultural industry criteria NY-T 393-2000 of the People's Republic of China (PRC) pollution-free food agricultural chemicals usage criteria of chemosynthesis; NY-T 394-2000 pollution-free food fertilizer usage criteria).Research, little, the stable in properties of exploitation dosage, plant growth regulator easy to use have become the development trend in this field, and nontoxic, have no side effect and the highly effective and safe product that do not influence plant eubolism approach and mode is the emphasis difficult point of plant growth regulator research.
Summary of the invention
The purpose of this invention is to provide a kind of metal derivative of chlorophyll and hydrolysate thereof that contains as the application of new plant growth regulator.The present invention be a kind of nontoxic, have no side effect and do not influence the plant growth regulator of the highly effective and safe of plant eubolism approach and mode.
The scheme of finishing the foregoing invention task is: a kind of metal derivative of chlorophyll and hydrolysate thereof that contains is as the application of new plant growth regulator.The metal derivative that contains chlorophyll and hydrolysate thereof as plant growth regulator of the present invention, be chlorophyll metalloid chelate, comprise chlorophyll metallo-chelate, chlorophyllin metallo-chelate and by the metallo-chelate of the separating obtained monomer component of chlorophyllin (mixture).In these metallo-chelates, preferred chlorophyllin metallo-chelate.
Chlorophyll metallo-chelate of the present invention all has good plant growth regulating activity.
Chlorophyll metallo-chelate of the present invention, can conventional method with chlorophyll or chlorophyllin or pheophorbide or in the chlorophyllin component separating obtained monomer component be that part and transition metal salt preparation get.
Chlorophyll metallo-chelate of the present invention, according to the source of part, prepared metallo-chelate i.e. mixture, also monomeric compound.Mixture types of metals chelate comprises: chlorophyll and chlorophyllin are the metallo-chelate of part preparation; The monomer type metallo-chelate comprises: separating obtained monomer component is the metallo-chelate of part preparation in the chlorophyllin component.The preferred mixture types of metals chelate cheap and easy to get of the present invention.
Chlorophyll metallo-chelate of the present invention, used chlorophyll are to be the green pigment with the female ring of pheophytin (phorbin) structure that raw material extracts with the natural plants.
Chlorophyllin metallo-chelate of the present invention, used chlorophyllin are the multiple dihydro porphin mixture of ingredients that chlorophyll obtains through hydrolysis.
Monomer type chlorophyllin metallo-chelate of the present invention, used monomer chlorophyllin composition comprises: chlorin-e4, chlorin-e6 and pheophorbide a mono-methyl.
The plant growth regulator that contains the chlorophyll metal derivative of the present invention, the metal ion of use comprises: the transition metal manganese of oxidation state divalence or trivalent, iron, copper, zinc ion, preferred manganic ion and iron ion.The monomer component of transition metal ions and chlorophyll or chlorophyllin or chlorophyllin generates the chelate of mol ratio 1: 1 or 1: 2.
The plant growth regulator that contains the chlorophyll metal derivative of the present invention is that the solution that is mixed with the chlorophyll metal derivative of 0.001ppm～100ppm uses.According to the specific requirement of working concentration, can directly add the water preparation; Higher or the not diffluent solution of desired concn can the small amount of alkali sample dissolution or earlier with the small amount of ethanol dissolving, thin up is formulated then.
The explanation of invention technology
In vitro test of the present invention is unexpected to be found, employed new plant growth regulator (concentration: 1.0ppm～100ppm) can significantly suppress chlorophyllase to chlorophyllous degraded (concentration of substrate 324ppm, 0.36mmol/L), its IC
50Scope: 13.84～85.46ppm, and inhibiting rate increases progressively according to the rising of sample concentration, has typical state of conflict and suppresses characteristics; And comparative trial finds that haematoporphyrin (Hemin, hemin, chlorination PORPHYRIN IRON, ferriporphyrin) solution does not have influence to chlorophyllase degraded chlorophyll, and the haematoporphyrin molecule of similar structures does not have external chlorophyll enzyme inhibition activity.
The used new plant growth regulator solution of the present invention that mung bean chlorophyllase in seedling stage suppresses to experiment showed, 0.1ppm concentration has obvious inhibitory action to the chlorophyllase activity of mung bean blade, and the chlorophyllase inhibiting rate of series compound reaches 4.25%～24.44%.The plant physiology evidence, the new plant growth regulator that the present invention uses not only can reduce the chlorophyllase vigor, improve chlorophyll content of plant, can also induce the rising of plant leaf blade SOD, CAT and POD vigor under the salt stress, promote the content of polyphenoils proline to raise simultaneously, thereby alleviate the oxidative damage of plant leaf blade under the salt stress, strengthen crop anti-adversity.
Cultivation and field experiment show that 0.001ppm～10.0ppm solution can be used for seed soaking, cuttage or crop field irrigation; 0.1ppm～10.0ppm solution is used for spraying; 2.0ppm～100ppm solution is used for trunk injection, to promote seed germination, improve germination rate, increase the long radical of root, to promote the adventive root growth, accelerate growth of seedling, strengthen resistance, improve chlorophyll content, increase output, delay the leaf lawn removing jaundice fallen leaves time, improve the flowers and trees appreciation effect.
The new plant growth regulator solution trial that the present invention uses shows, solution pH scope 5.0～7.0 under working concentration of preparation, SOLUTION PROPERTIES is more stable, solution preparation lucifuge condition still reaches 90% above content after following 2 hours, can bring into play the action effect of plant growth regulator, and being convenient for carrying transportation and use, product is practical.The haematoporphyrin contrast solution that (lucifuge) concentration is close under the same terms after 2 hours content reduce more than half, 50% of the not enough initial concentration of actual concentrations.
The new plant growth regulator that the present invention uses is with existing technology of preparing production, the various method simple process of technology path maturation, and raw material sources are abundant, with low cost, the chlorophyll that namely can the natural green plant leaf blade extracts is feedstock production, the chlorophyllin series products of also can market buying such as chlorophyllin magnesium sodium salt (or claim: chlorophyll magnesium sodium salt), the copper chlorophyllin sodium salt (or claims: sodium copper chlorophyllin), sodium iron chlorophyllin salt (or claim: sodium-iron-chlorophyllin) or pheophorbide be feedstock production, the preparation of its chlorophyllin class elemental metals chelate can separate be obtained by mixture types of metals chelate product, also can the monomer part and reacting metal salt make.
Advantage of the present invention
1, safety non-toxic, the new plant growth regulator source that the present invention uses is natural, and no people and animals' toxicity more do not comprise other harmful components that plant can't metabolism, and the low consumption of dosage is few, is difficult for accumulating residual, so safety is good.
2, active strong, effective, and the effect performance is more extensive, as improving chlorophyll content of plant, hestening rooting, raising percentage of seedgermination, strengthen crop anti-adversity, increasing crop yield etc.
3, environmental protection can reduce fertilizer amount in using in right amount under guaranteeing the output prerequisite, effectively reduce the various harm of chemicals.
4, the convenient use is suitable for the multiple common using method of agriculture and forestry, as seed soaking (immersion), irrigation, foliage spray, injection etc.It is more wide in range that dose concentration requires, and positive and negatively uses the also unlikely ill effect such as the obvious underproduction that causes near concentration deviation more than 10 times.
5, stable in properties cheap and easy to get, product prepares production easily, and raw material sources are extensively with low cost, and product property is stablized easy to use, and the quality of production is controlled reliable, is easy to apply.
Embodiment
Embodiment 1: chlorophyll enzyme inhibition activity (in vitro test) accurately takes by weighing the acetone powder 1.00g that is made by fresh green gram leaf, with 4 ℃ of centrifugal 10min behind 25mL phosphate buffer (pH 7.0～7.5) the extracting 4.5h, get supernatant and be chlorophyllase liquid, organized enzyme concentration: 0.14mmol/L, substrate chlorophyll concentration: 130ppm.Measure the chlorophyllase inhibiting rate (Gan Zhijun of each concentration chlorophyllin metal derivative sample solution of 0.4ppm～25.0ppm, Wang Xiaoyun, Zou Qi. wheat chlorophyllase biochemical kinetics characteristic research. northwest Botany Gazette, 2003,23 (5): 750～754.), and calculate IC
50Value, series of samples IC
50The results are shown in following table
The chlorophyll enzyme inhibition activity IC of series of samples
50 *
*: concentration of substrate 324ppm (0.36mM)
Embodiment 2: maize seed soaking germination rate and seedling growing way time: in April, 2009; Corn variety: come together sweet No. one; Sample: chlorination phyllo-iron and chlorination chlorophyllin iron; Test method: 0.001ppm～1.0ppm sample solution maize seed soaking 18 hours, clear water immersion group changes over to afterwards and cultivates in the identical soil of soil property and fertility for contrast (CK), waters once in per 2 days.After 10 days with compare, examine or check the difference of its germination rate, plant height and main root long (being all mean value), the results are shown in following table
Maize seed soaking germination rate and the contrast of seedling growing way
The bud ratio of each concentration of experimental group, plant height and root length compare with CK that all there were significant differences, and experimental group not only growing way is good, and root system abundant flourishing, lateral root is many, main root long (long near 2 times of roots of control group).
Embodiment 3: body inner chlorophyll enzyme suppresses experiment, choose full seed, mung bean seed of the same size with new plant growth regulator 0.1ppm solution immersion 4h of the present invention, 28 ℃ of following presoaking and germinatings of room temperature, select the consistent mung bean that germinates, 0.1ppm water culture in illumination box is when growing 1～2 pair of true leaf (after about 8 days), make acetone powder with its blade, supporting with water planting is blank (inhibiting rate: 0%), measure each processed group chlorophyllase activity, get the following table result
Embodiment 4: the test of corn field planting, place: Lao Guan village, Yuanan County, Hubei, corn variety: beautiful No. two of brocade.The medium fertility hillside fields, test area 133 square meters are established the equal area contrast.Experimental group seedling stage (during 6～7 leaves) foliage spray 1.0ppm chlorination chlorophyllin iron sample solution 1 time grows to the shooting stage (during 8～9 leaves) and sprays the 2nd time with same concentrations, and the typhon mouth phase sprays 1 time again, sprays altogether 3 times (concentration is identical).Actual production, 105.1 kilograms of experimental group, 90.0 kilograms of control groups, volume increase 16.7%.
Embodiment 5: emerge under the maize seed soaking cultivation drought stress and growing way in August, 2009, (0.001ppm～5.0ppm) seed soaking was handled corn seed (kind: come together sweet No.) in 18 hours to new plant growth regulator series of samples of the present invention, was contrast (CK) with clear water immersion group with variable concentrations.Be seeded in after the seed soaking in the identical soil of soil property and fertility, water during sowing 1 time, the hot summer weather environment and stop to water (the simulating drought condition) that place natural lighting to ventilate then, the average emergence rate of examination, height of seedling and main root are long after 12 days, and measure and respectively organize the chlorophyll average content.The results are shown in following table
Annotate 1.: chlorophyll content is SPAD value (unit: mg/g)
The result proves that new plant growth regulator of the present invention namely has good plant growth regulation under low concentration, can significantly improve bud ratio and plant height, and wherein main root is long increases and control group difference and remarkable.
Embodiment 6: the aloe leaf surface spraying test, and aloe barbadensis Miller, with the 1st foliage spray of 3.0ppm chlorination chlorophyllin iron sample solution, clear water sprays and is control group during 11 months planting seasons.Later every interval January sprays 1 time with same concentrations, stops to spray sample solution after spraying 4 times continuously, other fertilisings, waters as usual.
Observe during 18 months vegetative period, the about half blade of each strain of control group has the black fleck, and the basic immaculate in the full surface of experimental group blade.Experimental group and control group (each 10 strain) compare, the average blade of every strain, and experimental group: 13.1/strain, control group: 11.5/strain; Maximum blade (1/strain) average length, experimental group: 85 ± 2.2cm, control group: 79 ± 1.7cm (experimental group balanced growth 7.6%); The average sheet of maximum blade (1/strain) is heavy, experimental group: 512.3g, control group: 465.5g (experimental group increases by 10.06%).
Embodiment 7: chlorophyllin iron (test solution) is induced the generation of mung bean hypocotyl adventive root, choose the mung bean seedlings that growth is unified, height of seedling is 7～8cm, saving down from first pair of true leaf, 3cm cuts, to transplant a cutting and insert respectively in 1.0ppm～0.1ppm test solution and the distilled water, renew solution every day one time, coprocessing 6 days.Handle second day its adventive root notable difference takes place namely to show, measured in the 6th day, get the average indefinite radical of every cutting seedling and average adventive root length overall, the results are shown in following table
Embodiment 8: chlorination chlorophyllin a mono-methyl closes iron (test solution) to the protection experiment of green bean-leaf oxidative damage under the salt stress, 150mmolL
-1NaCl is condition of salt stress, with Hogland nutrient solution configuration 10ppm, 1ppm, 0.1ppm chlorination chlorophyllin a mono-methyl closes the iron test solution and cultivated mung bean seedlings eight days, be that (chlorination chlorophyllin a mono-methyl closes iron: 0 concentration), it is as shown in the table to handle the data of measuring in the 8th day in contrast with the Hogland nutrient solution that does not add test solution
Find to add chlorination chlorophyllin a mono-methyl and close the chlorophyll content that each concentration test solution of iron can significantly strengthen blade, reduce film fat peroxidating product malonaldehyde (MDA) accumulation, increase the content of polyphenoils proline, and increase POD (peroxidase), SOD (superoxide dismutase) that can be in various degree and CAT (catalase) the antioxidase vigor that is.
Embodiment 9: land for growing field crops rape leaf surface spraying test, place: Li Qiao village, Liu Lang town, Wuhu County, Anhui Province, heavy viscosity lake mud field, slant acidity soil (topsoil pH 6.0), middle fertility.
Sample: chlorination chlorophyllin iron; Solution concentration 0.8ppm; Test area 667m
2(experimental group) method: foliage-spray, spray altogether 3 times, wherein rape seedling sprays 1 time, reproductive stage and sprays (15 days at interval) 2 times; Spray water in same rape continuous cropping field and compare (area 667m
2, control group), fertilizer amount and water the water yield and respectively organize constant.Observe growing way and damage by disease and insect situation, measure crucial physiology and economic data, multianalysis test effect.
Rape growing way and chlorophyll content contrast spray back the 15th day (initial bloom stage) mensuration rape plant, blade growing way and chlorophyll content for the third time and the results are shown in following table
The mensuration contrast of the whole strain of Wuhu rape initial bloom stage and blade
*Fall 14 blade data
Experimental group and control group relatively, no matter all there were significant differences for whole strain and blade growing way, that experimental group shows as is strongr, tall and big, color depth and full.Chlorophyll content difference up 50% more than illustrates under equal conditions, and new plant growth regulator of the present invention can improve dish quality in seedling stage, increase chlorophyll content, strengthen photosynthesis, promote seedling leaf number and functional leaf to increase, promote it to nourish and grow and reproductive growth.
Output and quality contrast, experimental group (every 667m
2) rape seed effective yield 255.3Kg, control group output 197.7Kg, experimental group and contrast ratio volume increase 29.14%.
Oilseed prod attributional analysis (Ministry of Agriculture's oil plant and quality of item supervision and inspection center) is the result show, experimental group and control group quality indifference prove that plant growth regulator of the present invention does not have any adverse effect to the quality of rape seed when improving output.
Damage by disease and insect situation experimental group does not have obvious damage by disease and insect and takes place, its freeze injury of synthesis result, downy mildew, stalk break rate and to split the bar rate very more remarkable than contrast component 11.70%, 17.20%, 13.1%, 6.70% difference that do not descend.Show that foliage-spray chlorination chlorophyllin iron can improve the rape physiological vigor and promote growth, improve rape resistance.
After economic characters contrast experiment's group sprays 1 time, thick, the branch of seedling stage rape plant height, collar, secondary branch, main shaft are long, spindle angular fruit number, stem total leaf number, greenery number and single-strain fresh weight etc. are all very remarkable with the variation that control group relatively takes place, and increase 11.7cm, 0.215cm, 1.45,3.95,1.2cm, 8.45,2.83,3.04 and 109.15g respectively than contrast; Finish spray for three times after, the variation that reproductive stage rape plant height, collar be thick, branch, secondary branch, main shaft are long, spindle angular fruit number, stem total leaf number, greenery number and single-strain fresh weight take place, still all very more remarkable than seedling spraying variation once, do not increase 20.85cm, 0.48cm, 2.55,8.35,3.35cm, 13.8,5.9,8.02 and 159.95g than the contrast component.
Embodiment 10: rape trunk injection hestening rooting is improved growing way test site, soil property, fertility with embodiment 9, in the land for growing field crops rape that does not have to spray, choose rape 20 strains in squaring period (excellent choose for order to weak) of growing way optimum, set up badge card in order, be control group with odd number 10 strains, even number 10 strains are experimental group, and the every strain of experimental group rape is in the 3rd side shoot place injection 6.0ppm chlorination 1.5 milliliters of ferrous solutions of chlorophyllin (dosage: 0.009 milligram/strain).
The species test result, up to each later stage, the plant growing way all shows the obvious growth advantage to the experimental group rape from injection.The high contrast of plant: the average plant of experimental group is up to 187.43 ± 2.3cm, control group 163.43 ± 2.6cm; Growing way: experimental group cotyledon hypertrophy, stem stalk are sturdy, the big pod grain of angle fruit is many.
The root system contrast, the experimental group well developed root system, the root mass amount is big, and absorptivity is strong.Obvious with contrast difference, see the following form
The stem institutional framework: the prosperity of experimental group rape stem xylem, the degree of lignification height, the conduit wall thickness, the conduit number is many in each vascular bundle, is conducive to transportation and the supply of moisture and mineral nutrition, for rape growth provides good material guarantee.
Embodiment 11: the wheat field planting test of different fertilizer consumption, place: the precious Hua Zhenfeng altar in Jurong City, Jiangsu Province village; Soil property neutrality (pH7.0), middle fertility cultivated land 400 square meters are turned over before the sowing and are divided equally 4 (100 square meters/piece) after adding conventional fertilizer; Be divided into test and contrast two and organize control group greatly: the general planting method, 4.75 kilograms of chemical fertilizer application total amounts are made base manure and are topdressed 2 conventional dispensings of branch with urea (2 kilograms), diammonium phosphate (2 kilograms) and potassium chloride (0.75 kilogram); The same control group of experimental group fertilizer consumption is divided into 3 groups again according to fertilizer amount: full fertilizer group (4.75 kilograms of chemical fertilizer), half fertile group (chemical fertilizer respectively reduces by half, total 2.38 kilograms), the fertile group of nothing (not using chemical fertilizer).Test method: all experimental group are sowed after 18 hours with 0.3ppm chlorination chlorophyllin molten iron solution seed soaking, in the process of growth respectively at preceding 2 days of the phase of surviving the winter, survive the winter after date 7 days, vegetative period and blooming stage with the foliage spray of 1.6ppm chlorination chlorophyllin molten iron solution.Result of the test is as follows:
Spray back 7 days examination growing ways in seedling stage for the 3rd time, measure its plant height, vane thickness and chlorophyll content, see the following form
Experimental group wheat seeding plant height and vane thickness all are better than contrast, and increase progressively and (increase progressively 6.28%～18.2% plant height respectively according to fertile amount of application; 0.9%～16.9% thickness), show that chlorination chlorophyllin iron has the obvious growth regulating action.Yet, half fertile experimental group the highest (increasing by 13.5%), complete fertile group appear in chlorophyll content, and to take second place, does not have fertile group minimum even be lower than the result of contrast (reduction by 8.8%), illustrate that fertilizer nutrition and the effect of chlorination chlorophyllin iron growth regulating have different role and influence to the wheat seeding growing way, produce different effects, coordinate the two ratio and can reach good technical effect more.
Cut harvest time and receive species test in preceding 2 days, the relatively real per unit area yield of receiving of each group is calculated in grouping harvesting back, and analyzing relatively, the wheat micronutrient levels changes.Species test: each group is gathered in 1.21 square meters respectively, statistics number of productive ear, grain number per spike, thousand kernel weight, and the real per unit area yield of receiving of each group sees the following form
Under the effect of chlorination chlorophyllin iron, increase the effective fringe of applying quantity of chemical fertilizer and increase progressively, but grain number per spike increases and then reduce (half fertile group grain number per spike maximum) earlier, thousand kernel weight and grain number per spike and contrast all no significant differences; Use chlorination chlorophyllin iron all can increase output (1.9%～27.8%), amount of increase in production and chemical fertilizer (base manure and topdress) amount of application there is no positive correlation, the real per unit area yield of receiving is with half fertile group the highest (volume increase reaches 27.8%), preliminary explanation chlorination chlorophyllin iron has the effect that reduces chemical fertilizer and increase output simultaneously, and good prospects for application should be arranged.
Assay determination wheat products micronutrient levels finds use chlorination chlorophyllin iron can increase Ca content, and other elements not to have significant change, illustrate that chlorination chlorophyllin iron does not influence the original basic distribution of crop metal trace element as plant growth regulator.
Each organizes wheat healthy trace elements with household content
Gathering in fetched earth the same day measures the remaining fertility of soil, get the even degree of depth soil sample of 20cm in every group of plot plow layer, mensuration is respectively distinguished soil N, P, K constituent content (residual volume) reaches and sees the following form with control group residual volume ratio with TFC series chemical fertilizer in soil tacheometer (the prosperous and powerful analytical instrument in Beijing manufacturing center).
Harvesting back soil fertility residual volume and residual volume ratio
The soil fertility of planting latter stage is the comprehensive embodiment of fertilizer input and crop utilization effect, and the former increases fertility remnants, and the latter then reduces remnants, and residual volume more little (residual volume is lower than more) crop fertility availability is more high, and the fertility waste is more few; Otherwise availability is more low, the waste is more big.Test is respectively organized the fertilizer residual volume all less than control group, shows the fertility efficient of using chlorination chlorophyllin iron can increase wheat crop, to obtain to increase the effect of output; But fertility utilization ratio and fertility height (what are used) have notable difference, and its difference shows as under the different soil fertility condition: during fertility abundance (complete fertile group), the fertile utilization ratio of K is the highest; When fertility weakens (half fertilizer is organized and do not had fertilizer and organize), the fertile utilization ratio of P is the highest.The fertile residual volume prompting of higher N uses chlorination chlorophyllin iron can significantly reduce the fertile usage amount (reducing by more than 50) of N.The highest prompting of half fertile group output, change fertilizer N, P and K ratio and (significantly reduce N fertilizer, suitably reduce K fertilizer) and reduce the possibility that the total amount of application of fertilizer has continuation raising output, its practical significance is that the growth regulator of trace namely might be saved the chemical fertilizer of appreciable amount, this to reduce cost, to reduce environmental pollution significant, will produce considerable society and economic benefit.
Embodiment 12: rice field plantation, place: Lao Guan village, Yuanan County, Hubei, kind: No. 22 seed rice of farming earlier, fertility irrigated land such as low in the slant acidity, about 2730 square meters of the test gross area, wherein control group area 334 square meters.Experimental group (2 groups) is respectively: seed soaking-sprinkling group (800 square meter) and irrigation-sprinkling group (1668 square meter).Test method: seed soaking, 1 kilogram of seed rice soaked 24 hours with 2 liters of 1.5ppm chlorination chlorophyllin iron sample solution; Irrigate, chlorination chlorophyllin iron sample is mixed with earlier than concentrated solution, is added several times according to current by the rice field water filling port, reaches the irrigation final concentration with injected water volume to requiring the depth of water namely to dilute; Spray the foliage spray of 2.0ppm chlorination chlorophyllin iron sample solution.Seed soaking-sprinkling group: seed soaking on April 19th, 2009, spray after 10 days the 1st time in transplantation of seedlings, spray tillering stage the 2nd time, spray the 3rd time before the flowering booting, each per 667 square meters spray about 30 liters of solution; Irrigation-sprinkling group: commonsense method is cultivated shoot transplanting equipment and is sprayed after 10 days the 1st time, irrigates tillering stage 1 time (sample 50mg pours water dark 0.03 meter, the about 0.001ppm of concentration), sprays the 2nd time before the flowering booting, and each sprinkling amount is the same.Control group: according to conventional process.
Observe each phase test treatment effect, compare with control group, during transplanting: seed soaking is handled with rice shoot (the comprising irrigation-sprinkling group seedling) difference of non-seed soaking obvious, the rice shoot plant height that seed soaking is handled on average increases by 16.2%, color depth (chlorophyll content improves 9.5%), shoot root is long increases by 61.3%, its blade colourity difference is retained to plant always change Huang before.
Ripe back is in piecemeal harvesting on September 12nd～16,2009.Weigh and calculate constituent parts output (the dried rice output of per 667 square meters), control group: 565.0 kilograms; Seed soaking-sprinkling group: 620.8 kilograms (volume increase 9.88%); Irrigation-sprinkling group: 582.9 kilograms (volume increase 3.17%).Synthesis result the analysis showed that, seed soaking, sprinkling and irrigation all are effective processing methods, sample is minimum, operation is the simplest and effect is obvious but seed soaking is used, function influence almost runs through paddy rice whole vegetative period, overall efficiency is the highest, and the irrigation of non-seed soaking-sprinkling group amount of increase in production is obviously not as good as seed soaking-sprinkling group; Seed soaking-spray the combination ratio to irrigate-spray the combined method better effects if is more suitable for the plantation of paddy rice.
Embodiment 13: growing way experiment under the drought stress of wheat seed soaking back, the wheat seed of " raising wheat 158 " is with 10ppm, 1ppm, 0.1ppm chlorination chlorophyllin ferrous solution seed soaking, be that the osmotic stress condition is carried out osmotic stress and handled with 20% (w/v) PEG-6000, be contrast with water, cultivate observed the wheat seed average germination percentage on the 6th day and average root long, get the following table result
The result shows, under drought condition, chlorination chlorophyllin ferrous solution of the present invention can effectively improve germination rate and significantly increase wheat root long to the processing of soaking seed of little wheat seeds.
Embodiment 14: the experiment of vegetables seed soaking germination rate, and vegetable species: a variety of Chinese cabbage, flowering radish, carrot, cucumber, asparagus lettuce, cowpea, all purchase the green neck kind industry Co., Ltd in Nanjing.Each vegetable seeds is in 1ppm, and 0.1ppm soaks 8h in the 0.01ppm chlorination chlorophyllin ferrous solution, and clear water soaks for contrast, cultivates in Turnover Box with the soil of identical soil property and fertility, waters once in per 2 days, and its germination rate of record gets following table germination rate result after 15 days,
The result shows, solution of the present invention is handled the seed soaking of vegetables kind, can effectively improve its germination rate, though various germination of vegetable seeds have nothing in common with each other with the seed soaking concentration, but the result of contrast does not all appear being weaker than in the variation of 100 times of concentration difference, illustrate that the technical method that the present invention adopts has the more wide in range scope of application, is conducive to apply.
Claims (5)
1. one kind contains the metal derivative of chlorophyll and hydrolysate thereof as the application of the plant growth regulator of chlorophyllase inhibition; It is characterized in that the metal ion in the described metal derivative is: the transition metal ions of oxidation state cupric, manganese, zinc or manganic, iron; Described chlorophyll is to be the green pigment with the female ring structure of pheophytin that raw material extracts with the natural plants;
The described metal derivative that contains chlorophyll and hydrolysate thereof is selected from: chlorophyll metallo-chelate, chlorophyllin metallo-chelate reach the metallo-chelate by the separating obtained monomer component of chlorophyllin mixture;
Described monomer component is selected from: chlorin-e4, chlorin-e6 or pheophorbide a mono-methyl.
2. the metal derivative of chlorophyll and hydrolysate thereof that contains according to claim 1 is as the application of the plant growth regulator of chlorophyllase inhibition, it is characterized in that the described metal derivative that contains chlorophyll and hydrolysate thereof is the chlorophyllin metallo-chelate.
3. the metal derivative of chlorophyll and hydrolysate thereof that contains according to claim 1 is characterized in that described metal ion is selected from: manganic ion and ferric ion as the application of the plant growth regulator of chlorophyllase inhibition.
4. the application of the plant growth regulator that suppresses as chlorophyllase according to the described metal derivative that contains chlorophyll and hydrolysate thereof of one of claim 1-3, it is characterized in that the monomer component of described transition metal ions and chlorophyll or chlorophyllin or chlorophyllin generates the chelate of mol ratio 1:1 or 1:2.
5. the described metal derivative of chlorophyll and hydrolysate thereof that contains of claim 4 is characterized in that as the application of the plant growth regulator of chlorophyllase inhibition this plant growth regulator is that the solution that is mixed with 0.001 ppm～100ppm uses.
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| CN111248217A (en) * | 2020-03-29 | 2020-06-09 | 邢宇俊 | Plant growth regulator composition containing chlorin iron and thidiazuron |
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