CN102268063A - Method for separating active materials from marine animals by freezing and concentrating - Google Patents
Method for separating active materials from marine animals by freezing and concentrating Download PDFInfo
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- CN102268063A CN102268063A CN201110163970XA CN201110163970A CN102268063A CN 102268063 A CN102268063 A CN 102268063A CN 201110163970X A CN201110163970X A CN 201110163970XA CN 201110163970 A CN201110163970 A CN 201110163970A CN 102268063 A CN102268063 A CN 102268063A
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Abstract
The invention relates to a method for separating active materials from marine animals by freezing and concentrating, which comprises: preparing hydrolyzate of aquatic proteins by enzymolysis of aquatic raw materials, wherein the hydrolyzate of aquatic proteins contains micro molecular amino acids and macro molecular active materials such as polypeptides, proteins and polysaccharides; and freezing and concentrating the hydrolyzate of aquatic proteins, so that based on the principle that the diffusion speed in liquid of the micro molecular amino acids and the diffusion speed in liquid of the macro molecules are different, the micro molecular amino acids, which diffuse in a high speed, were retained in the liquid and concentrated while the macro molecular polypeptide, proteins and polysaccharides, which diffuse at a low speed, were wrapped or carried by ice crystals in a freezing and concentrating process so as to be separated out. In the invention, freezing and concentrating are used for separating the active materials from marine animals, so the hydrolyzate of aquatic proteins can be concentrated and separated at a low temperature, the bioactivity and flavor of the hydrolyzate can be retained to a maximum degree, the protein denaturation problem aroused by hot concentration is solved, the membrane pollution in a membrane separation process is avoided, and the influence on the activity of part of enzymes under action of a high-speed shearing force is avoided.
Description
Technical field
The invention belongs to field of processing of aquatic products, more specifically relate to a kind of method of freeze concentration separating marine biologically active substance.
Background technology
Macromolecular substance such as polypeptide, protein, polysaccharide have complicated chemical structure and very high biological activity in the marine organisms, are the affluent resources of healthcare product and new medicament screen.
Mainly concentrate on the membrane separation technique aspect about the isolating research of macromole active substance in the aquatic products enzymolysis solution at present.The color and luster and the local flavor of the liquid product that separation methods such as traditional screen filtration method, hot method of enrichment obtain are not good, often be prone to the precipitated and separated phenomenon in the put procedure, the Powdered goods that make with this solution that contains active substance often have that powder is coarse, appearance luster is not good, the raw meat bitter taste is arranged, easily the defective of moisture absorption caking.Membrane separation technique to the separation of marine bioactivity material than the efficient height of traditional method a lot, but be applied to poor effect on the suitability for industrialized production, film very easily pollutes, and separation costs is improved greatly.Secondly in the membrane sepn process, enzyme activity may be affected in the effect lower section of high speed shear power.
Summary of the invention
In order to overcome above-mentioned defective, effective separating marine biologically active substance, the invention provides a kind of method of freeze concentration separating marine biologically active substance, this method utilizes in the liquid that do not coexist of amino acid and peptide molecular weight velocity of diffusion different, under low-temperature atmosphere-pressure, pass through the decline of control refrigerant temperature, can not destroy under the active situation of macromolecular substance, make small molecules amino acid and macromole peptide, protein separation.
The present invention implements by following technical solution:
A kind of method of freeze concentration separating marine biologically active substance, be that the marine organisms aquatic product protein is obtained the aquatic product protein hydrolyzed solution behind enzymolysis, wherein contain small molecules amino acid and polypeptide, protein, macromole active substances such as polysaccharide, again the aquatic product protein hydrolyzed solution is handled through freeze concentration, by control refrigerant temperature and then crystallization control speed, utilize amino acid and peptide molecular weight not to coexist and have different velocity of diffusion in the liquid, small molecules amino acid velocity of diffusion is fast, be retained in the liquid and be concentrated, macromole peptide and protein velocity of diffusion are slow, in the freeze concentration crystallisation process, wrapped up or carry secretly, thereby separate by ice crystal.
Marine organisms among the present invention comprise fishery products such as fishes and shrimps Bei Zao, and the fishery products tankage, and marine organisms albumen such as fishes and shrimps Bei Zao through enzymolysis, are obtained containing the protein hydrolyte of macromole active substances such as small molecules amino acid and polypeptide, protein, polysaccharide.Through freeze concentration, refrigerant temperature is controlled at-18 ℃～-35 ℃ with said hydrolyzed liquid, collects the ice crystal of constantly separating out, and reaches isolating purpose.
Than prior art, the invention has the advantages that: the present invention is used for freeze concentration the separation of marine bioactivity material, can be with aquatic product protein hydrolysate concentrating and separating at low temperatures, the biological activity and the local flavor that at utmost keep hydrolysate, solve heat and concentrate the problem that makes protein denaturation, simultaneously, small-molecule substance such as amino acid etc. is concentrated, the membrane pollution problem of having avoided membrane sepn to produce, and under the effect of high speed shear power to the influence of part enzyme activity.
Embodiment
The method of freeze concentration separating marine biologically active substance, be to obtain the aquatic product protein hydrolyzed solution behind the raw materials of marine products pre-treatment enzymolysis, wherein contain macromole active substances such as small molecules amino acid and polypeptide, protein, polysaccharide, again the aquatic product protein hydrolyzed solution is handled through freeze concentration, utilize amino acid and the peptide molecular weight velocity of diffusion that do not coexist in the liquid different, micromolecular amino acid velocity of diffusion is fast, be retained in the liquid and be concentrated, macromole peptide, protein, polysaccharide velocity of diffusion are slow, in the freeze concentration crystallisation process, wrapped up, thereby separate by ice crystal.
Above-mentioned raw materials of marine products comprises fishery products such as fishes and shrimps shellfish, and the fishery products tankage.With protease hydrolyzed raw materials of marine products and filtration, obtain the aquatic product protein hydrolyzed solution.The aquatic product protein hydrolyzed solution is carried out freeze concentration, regulate refrigerant temperature to-18 ℃～-25 ℃,, make small molecules amino acid be retained in the liquid and be concentrated with crystallization control speed, the macromole active substance is wrapped up by ice crystal and is separated, and collects these ice crystals.After ice crystal was collected, centrifugal, the liquid that throws away is freeze concentration again, remaining ice crystal regathers, and dissolves, and contains macromolecular substance such as polypeptide, protein, polysaccharide in the solution after dissolving, can further carry out extraction separation after the preservation, the small molecules amino acid in the solution obtains purifying and concentrates.
Embodiment 1:
(1) getting with fresh abalone internal organs albumen is 5 kilograms of the raw materials of marine products of main component.
(2) add water and mix after, add protease hydrolyzed, obtain abalone internal organs protein hydrolyte, total solid concentration 5%, wherein aminoacids content accounts for total solids content 48%.
(3) abalone internal organs protein hydrolyte is carried out freeze concentration, refrigerant temperature is reduced to-18 ℃, and this moment, ice crystal was constantly grown.
(4) separate ice crystal, isolated liquid is refunded the concentration tank freeze concentration again, reaches 13% up to total solid concentration, records that aminoacids content accounts for 64% of total solids content in the solution this moment, and small molecules amino acid obtains purifying and concentrates.
Embodiment 2:
(1) gets with 5 kilograms of the protein raw materials of the tankage of fresh Harengulazunasi (Bleeker) and Harengulazunasi (Bleeker) gained after removing bone and grease and handling.
(2) add water and mix after, add protease hydrolyzed, obtain the scaled sardine protein hydrolyzed solution, total solid concentration 5.5%, wherein aminoacids content accounts for total solids content 51%.
(3) the scaled sardine protein hydrolyzed solution is carried out freeze concentration, refrigerant temperature is reduced to-25 ℃, and this moment, ice crystal was constantly grown.
(4) separate ice crystal, isolated liquid is refunded the concentration tank freeze concentration again, reaches 13.5% up to total solid concentration, records that aminoacids content accounts for 72% of total solids content in the solution this moment, and small molecules amino acid obtains purifying and concentrates.
Embodiment 3:
(1) gets with 5 kilograms of the shrimp protein raw materials of fresh shrimp head, shrimp shell gained after removing grease and handling.
(2) add water and mix after, add protease hydrolyzed, obtain the shrimp protein hydrolyzed solution, total solid concentration 5.8%, wherein aminoacids content accounts for total solids content 50%.
(3) the shrimp protein hydrolyzed solution is carried out freeze concentration, refrigerant temperature is reduced to-35 ℃, and this moment, ice crystal was constantly grown.
(4) separate ice crystal, isolated liquid is refunded the concentration tank freeze concentration again, reaches 13.9% up to total solid concentration, records that aminoacids content accounts for 78% of total solids content in the solution this moment, and small molecules amino acid obtains purifying and concentrates.
Embodiment 4:
(1) gets 5 kilograms of porphyra haitanensis protein raw materials.
(2) add water and mix after, add protease hydrolyzed, obtain the porphyra haitanensis protein hydrolyte, total solid concentration 5.8%, wherein aminoacids content accounts for total solids content 21%.
(3) the porphyra haitanensis protein hydrolyte is carried out freeze concentration, refrigerant temperature is reduced to-30 ℃, and this moment, ice crystal was constantly grown.
(4) separate ice crystal, isolated liquid is refunded the concentration tank freeze concentration again, reaches 12.8% up to total solid concentration, records that aminoacids content accounts for 42% of total solids content in the solution this moment, and small molecules amino acid obtains purifying and concentrates.
The above only is preferred embodiment of the present invention, and all equalizations of being done according to the present patent application claim change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. the method for a freeze concentration separating marine biologically active substance, the marine organisms aquatic product protein is obtained the aquatic product protein hydrolyzed solution behind enzymolysis, it is characterized in that: described method is that the aquatic product protein hydrolyzed solution is handled through freeze concentration, makes small molecules amino acid and macromole peptide, protein separation.
2. the method for a kind of freeze concentration separating marine biologically active substance according to claim 1 is characterized in that: the refrigerant temperature of described freeze concentration is-18 ℃～-35 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110163970XA CN102268063A (en) | 2011-06-17 | 2011-06-17 | Method for separating active materials from marine animals by freezing and concentrating |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110163970XA CN102268063A (en) | 2011-06-17 | 2011-06-17 | Method for separating active materials from marine animals by freezing and concentrating |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105817051A (en) * | 2016-03-30 | 2016-08-03 | 南通中国科学院海洋研究所海洋科学与技术研究发展中心 | Method for extracting micromolecular intracellular metabolite from nori |
| CN112592383A (en) * | 2020-12-09 | 2021-04-02 | 美泰科技（青岛）股份有限公司 | Method for high-power concentration of bioactive feed liquid at low temperature |
| CN116035190A (en) * | 2022-12-30 | 2023-05-02 | 浙江工业大学 | Mussel flavor base material produced by recycling protein from mussel processing byproducts and application |
-
2011
- 2011-06-17 CN CN201110163970XA patent/CN102268063A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105817051A (en) * | 2016-03-30 | 2016-08-03 | 南通中国科学院海洋研究所海洋科学与技术研究发展中心 | Method for extracting micromolecular intracellular metabolite from nori |
| CN105817051B (en) * | 2016-03-30 | 2018-03-06 | 南通中国科学院海洋研究所海洋科学与技术研究发展中心 | The method that small molecule intracellular metabolin is extracted from seaweed |
| CN112592383A (en) * | 2020-12-09 | 2021-04-02 | 美泰科技（青岛）股份有限公司 | Method for high-power concentration of bioactive feed liquid at low temperature |
| CN116035190A (en) * | 2022-12-30 | 2023-05-02 | 浙江工业大学 | Mussel flavor base material produced by recycling protein from mussel processing byproducts and application |
| CN116035190B (en) * | 2022-12-30 | 2024-10-18 | 浙江工业大学 | Mussel flavor base material produced by recycling protein from mussel processing byproducts and application |
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Application publication date: 20111207 |