CN102260635A - Freeze drying protective agent as well as preparation method and application thereof - Google Patents
Freeze drying protective agent as well as preparation method and application thereof Download PDFInfo
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- CN102260635A CN102260635A CN 201010185602 CN201010185602A CN102260635A CN 102260635 A CN102260635 A CN 102260635A CN 201010185602 CN201010185602 CN 201010185602 CN 201010185602 A CN201010185602 A CN 201010185602A CN 102260635 A CN102260635 A CN 102260635A
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Abstract
The invention provides a freeze drying protective agent. Based on 100 parts, the freeze drying protective agent is prepared from the following components in parts by weight: 12 parts-20 parts of degreased milk powder, 0.5 part-3 parts of sodium glutamate, 0.1 part-0.5 part of sodium ascorbate, 0.5 part-3 parts of glycerol, 0.05 part-0.3 part of cystine, 10 parts-20 parts of trehalose and the balance of water. The invention provides a method for preparing the freeze drying protective agent. The method comprises the following steps: mixing the components; and sintering for 30 minutes at the temperature of 100 DEG C so as to prepare the freeze drying protective agent. The freeze drying protective agent is applied to freeze drying of microorganisms, especially lactobacillus, thereby exerting the protective function of the freeze drying protective agent to the greatest extent and reducing the death of thallus in the freeze drying process so as to greatly improve the survival rate of thallus.
Description
Technical field
The present invention relates to a kind of freezing drying protective agent, specifically, the present invention relates to a kind of freezing drying protective agent, preparation method and application thereof of lactobacillus.
Background technology
Lyophilize is wet stock to be frozen at a lower temperature solid-state, then under high vacuum, with the direct distillation drying process of removing for gaseous state of solid-state moisture wherein, also claims sublimation drying.In freezing dry process, freezing and dry two processes can cause the passivation of damage, death and some zymoprotein molecule thereof of part microorganism cells.If the protective material selection is improper in the freeze-dry process, then fermentative activity descends.
Lactobacillus is one of microorganism resource important in the dairy industry microorganism, the effects such as balance, enhancing body immunizing power, reducing cholesterol level, alleviation lactose intolerance and the formation of inhibition tumour cell that have good fermentation character and improvement or regulate the enteric microorganism flora.Industry such as lactobacillus has been widely used in food, brewage.In how will producing better seed selection to good lactobacillus preserve, select suitable freezing drying protective agent particularly important.
The kind of various microorganisms is different with itself structure (having or not cyst membrane, pod membrane etc. as bacterium), cultural method is also variant; for the damage and the dead reduction as far as possible that make microorganism in the freezing dry process, the prescription of the freezing drying protective agent of various microorganisms also is not quite similar.Protective material commonly used is mainly milk, sucrose, gelatin etc., and prescription is simple, and defencive function is poor.If under 2 ℃ of-8 ℃ of conditions, preservation period has only the 4-6 month, how need preserve below-15 ℃.Add that the protectant development of traditional biological products relatively lags behind, make the prolonged preservation of finished product and transportation limited.So freezing drying protective agent is very urgent preferably to study a kind of effect.
Summary of the invention
The purpose of this invention is to provide freezing drying protective agent with preferable effect.This kind freezing drying protective agent is in the lyophilize of microorganism especially lactobacillus, and the protection effect increases significantly, and has improved the surviving rate of thalline greatly.
The invention provides a kind of freezing drying protective agent, 100 parts of freezing drying protective agents are prepared from by the component of following weight part:
12 parts-20 parts of skimmed milk powders,
0.5 part-3 parts of Sodium Glutamates,
0.1 part-0.5 part of sodium ascorbate,
0.5 part-3 parts of glycerine,
0.05 part-0.3 part of Gelucystine,
10 parts-20 parts of trehaloses,
Other is supplied by water.
In the common component of freezing drying protective agent, glycerine can promote lyophilize handle in the catalase renaturation, and rise to 0.8% when glycerol concentration, the catalase recoverability is complete.Skim-milk can promote distillation.Compare with sucrose, the second-order transition temperature of trehalose is higher, and hydroscopicity is lower, and no reductibility can form a kind of weak glass and has good provide protection.Sugar is relevant with its concentration to proteinic provide protection, and in certain concentration range, the provide protection of sugar increases along with the rising of concentration usually; When reaching a certain concentration, provide protection reaches maximum value; After this increase the concentration of sugar again, the protection effect does not have remarkable increase, can reduce on the contrary sometimes.Amino acid is one of common protein protectant, can prevent that active principle is with the water vapour loss that distils.
In refrigerating process, can the raise temperature of subsiding of finished product of crystal type amino acid, the destruction that stops the protein structure that causes because of subsiding.Some tensio-active agents such as tween 80, sodium laurylsulfonate and xitix or reductive agent also play the certain protection effect to the protein in the freezing dry process.In addition, the autoxidation of product can consume the oxygen in freeze-drying sample interior and the environment; Put into electronics or hydrogen ion, as xitix etc., oxidation chain reaction in the freeze-drying sample capable of blocking, the inhibited oxidation enzymic activity prevents sample deterioration by oxidation in lyophilize and storage.
The contriver has at first carried out single factor experiment in the screening process to cryoprotectant, wherein CK is not for adding any protectant control group, and skimmed milk powder 5% is meant that it is that skim-milk, 95 grams are water that 5 grams are arranged in the 100 gram freezing drying protective agents in the row 1; By that analogy.Listed the viable count that adds under the different protective material conditions in test-results such as the table 2, table 2.
Table 1 single factor experiment level of factor table
| Freezing drying protective agent | CK | 1 | 2 | 3 |
| Skimmed milk powder | 0 | 5% | 10% | 20% |
| Sodium Glutamate | 0 | 0.5% | 1.5% | 3% |
| Sodium ascorbate | 0 | 0.1% | 0.3% | 0.5% |
| Glycerine | 0 | 0.5% | 1.5% | 3% |
| Gelucystine | 0 | 0.1% | 0.2% | 0.3% |
| Trehalose | 0 | 10% | 15% | 20% |
Table 2 freezing drying protective agent single factor experiment result (* 10
9Cfu/g)
| Freezing drying protective agent | CK | 1 | 2 | 3 |
| Skimmed milk powder | 0.142 | 6.35 | 6.40 | 6.51 |
| Sodium Glutamate | 0.142 | 6.08 | 6.12 | 6.33 |
| Sodium ascorbate | 0.142 | 6.43 | 6.48 | 6.56 |
| Glycerine | 0.142 | 6.12 | 6.23 | 6.44 |
| Gelucystine | 0.142 | 6.40 | 6.52 | 6.58 |
| Trehalose | 0.142 | 6.59 | 6.66 | 6.78 |
Test-results shows that 6 kinds of different protective material components all have provide protection for thalline, adds in protectant sample viable count and is much higher than and does not add protectant blank.And the provide protection for thalline in freezing dry process of Gelucystine, sodium ascorbate and trehalose obviously is better than other compositions.Based on this, select these three factors to carry out orthogonal test, further determine best protective material prescription.Orthogonal experiments sees Table 3:
Table 3 lactobacillus lyophilized vaccine orthogonal experiments
| Test number | Gelucystine A | Sodium ascorbate B | Trehalose C | Empty row D | Viable count (* 10 9cfu/g） |
| 1 | 0.1% | 0.1% | 10% | 1 | 6.08 |
| 2 | 0.1% | 0.3% | 15% | 2 | 6.15 |
| 3 | 0.1% | 0.5% | 20% | 3 | 6.88 |
| 4 | 0.2% | 0.1% | 15% | 3 | 6.33 |
| 5 | 0.2% | 0.3% | 20% | 1 | 7.03 |
| 6 | 0.2% | 0.5% | 10% | 2 | 6.23 |
| 7 | 0.3% | 0.1% | 20% | 2 | 6.76 |
| 8 | 0.3% | 0.3% | 10% | 1 | 6.07 |
| 9 | 0.3% | 0.5% | 15% | 3 | 6.12 |
| K1 | 6.370 | 6.390 | 6.127 | 6.410 | ? |
| K2 | 6.530 | 6.417 | 6.200 | 6.380 | ? |
| K3 | 6.317 | 6.410 | 6.890 | 6.427 | ? |
| R | 0.213 | 0.027 | 0.763 | 0.047 | ? |
By above quadrature analysis result as can be known, the influence of trehalose is very remarkable, is main factor.Sodium ascorbate influence minimum, not remarkable.Therefore the size that influences of three factors is followed successively by: trehalose〉Gelucystine〉sodium ascorbate.The optimum level of this three factor is combined as: A2B2C3, under this condition after the lyophilize thalline survival number be 7.03 * 10
9Cfu/g.
The contriver finds through test of many times; each component in the freezing drying protective agent provided by the invention has all been brought into play effect separately; act synergistically mutually simultaneously, brought into play the defencive function of freezing drying protective agent to greatest extent, reduce the death of thalline in freezing dry process.Compare with existing frozen-dried protective agent prescription, trehalose that adds among the present invention and Gelucystine are to the survival rate effect of increasing significantly of thalline.
The invention provides a kind of method for preparing freezing drying protective agent; with 12 parts-20 parts of skimmed milk powders, 0.5 part-3 parts of Sodium Glutamates; 0.1 part-0.5 part of sodium ascorbate; 0.5 part-3 parts of glycerine; 0.05 part-0.3 part of Gelucystine; 10 parts-20 parts of trehaloses are soluble in water, are that 100 ℃ sterilization is prepared from through 30 minutes temperature.
The present invention also provides the application of a kind of freezing drying protective agent in microbial freezing drying.
Further, the invention provides the application of a kind of freezing drying protective agent in lactobacillus.Above freezing drying protective agent has preferable protection effect to lactobacillus in freezing dry process.
Utilize the freezing drying protective agent among the present invention can make lactobacillus lyophilize starter, the preparation method comprises the steps: lactobacillus through centrifugal enrichment, mixes under sterile state in ratio and the freezing drying protective agent of mass ratio 1:1; Is that storage strengthened the survival rate of lactobacillus in freezing dry process to strengthen the freezing tolerance of somatic cells in 12 hours in 4 ℃ the environment with the microbial inoculum that obtains at storage temperature.
Adopt the lactobacillus lyophilize starter of above-mentioned freezing drying protective agent production to be inoculated in the cow's milk of sterilization, under 43 ℃ condition, carry out fermenting experiment, fermentation 4.5-5h, titration acidity 〉=65 ° T, pH≤4.70, the sense organ local flavor is all good.
According to 1:10(w/v) lactobacillus lyophilize starter is dissolved in (121 ℃ of sterilizations, in virgin Chinese protein peptone water 15min), do suitable dilution then, be poured into (115 ℃ of sterilizations, on MRS substratum 15min), behind 37 ℃ of cultivation 48-72h, cell concentration is all greater than 1.0 * 10 after testing
9Cfu/g.
Wherein, above lactobacillus can be the lactobacillus bulgaricus subspecies, separates in the tradition milk-product of the Inner Mongol, and Inner Mongol tradition milk-product can be by being purchased or gathering acquisition to the pastoral area.
Embodiment
Below in conjunction with specific embodiments method of the present invention is done more detailed description.It will be appreciated by those skilled in the art that following embodiment all is used for the present invention's scope required for protection is carried out the description of exemplary, summarize the relative scope of each parameter of the present invention, thereby it can not be interpreted as a kind of concrete restriction of the present invention with this.
Table 4. freezing drying protective agent prescription
| Component (unit: gram) | Prescription 1 | Prescription 2 | Prescription 3 |
| Skimmed milk powder | 15±1 | 19±1 | 13±1 |
| Sodium Glutamate | 2.5±0.5 | 0.6±0.1 | 0.7±0.1 |
| Sodium ascorbate | 0.15±0.05 | 0.4±0.1 | 0.3±0.1 |
| Glycerine | 2.5±0.5 | 1.5±0.5 | 0.6±0.1 |
| Gelucystine | 0.25±0.05 | 0.2±0.05 | 0.1±0.05 |
| Trehalose | 15±1 | 19±1 | 11±1 |
| Water | 64.6±1 | 59.3±1 | 74.3±1 |
In table 4 prescription ± number, the limit of error that allows during only representative is tested.
Embodiment 1
The component of freezing drying protective agent as in the table 4 prescription 1 shown in,
Preparation method's following steps:
1) mixes according to the component of above-mentioned prescription 1;
2) above-mentioned freezing drying protective agent is sterilized, the temperature of sterilization is 100 ℃, and the time is 30 minutes.
The above-mentioned freezing drying protective agent for preparing is applied in the lactobacillus, lactobacillus is had preferable protection effect in freezing dry process.
Preparation has the lactobacillus fermentation agent of above-mentioned freezing drying protective agent: at first with centrifugal enrichment to lactobacillus bulgaricus join in the above-mentioned freezing drying protective agent in the ratio of mass ratio 1:1, under sterile state, both are mixed.Then the lactobacillus bulgaricus proliferated culture medium for preparing is stored in 4 ℃ of environment and stored 12 hours.
The dry matter content that adopts the lactobacillus bulgaricus lyophilize starter of above-mentioned freezing drying protective agent production to be inoculated in sterilization is to carry out fermenting experiment in 11.5% the sweet milk under 43 ℃ condition; fermented 4.5-5 hour; titration acidity 〉=65 ° T, pH≤4.70, the sense organ local flavor is all good.
According to 1:10(w/v) the lactobacillus bulgaricus freeze dried fermenting preparation is dissolved in (121 ℃ of sterilizations, 15 minutes) virgin Chinese protein peptone water in, do suitable dilution then, after 48-72 hour, cell concentration is 2.7 * 10 after testing in the last 37 ℃ of cultivations of milk-acid bacteria substratum (MRS substratum)
9Cfu/g.
Embodiment 2
The component of freezing drying protective agent as in the table 4 prescription 2 shown in,
Preparation method's following steps:
1) mixes according to the component of above-mentioned prescription 2;
2) above-mentioned freezing drying protective agent is sterilized, the temperature of sterilization is 100 ℃, and the time is 30 minutes.
The above-mentioned freezing drying protective agent for preparing is applied in the lactobacillus, lactobacillus is had preferable protection effect in freezing dry process.
Preparation has the lactobacillus fermentation agent of above-mentioned freezing drying protective agent: at first with centrifugal enrichment to lactobacillus bulgaricus join in the above-mentioned freezing drying protective agent in the ratio of mass ratio 1:1, under sterile state, both are mixed.Then the lactobacillus bulgaricus proliferated culture medium for preparing is stored in 4 ℃ of environment and stored 12 hours.
The lactobacillus bulgaricus freeze dried fermenting preparation that adopts above-mentioned freezing drying protective agent production is to carry out fermenting experiment in 11.5% the whole-milk under 43 ℃ condition with the dry matter content that is inoculated in sterilization in right amount; fermented 4.5-5 hour; titration acidity 〉=65 ° T, pH≤4.70, the sense organ local flavor is all good.
According to 1:10(w/v) the fermentation using lactobacillus bulgaricus agent is dissolved in (121 ℃ of sterilizations, 15 minutes) virgin Chinese protein peptone water in, do suitable dilution then, cultivated 48-72 hour for last 37 ℃ at milk-acid bacteria substratum (MRS substratum), cell concentration is 6.7 * 10
9Cfu/g.
Embodiment 3
The component of freezing drying protective agent as in the table 4 prescription 3 shown in,
Preparation method's following steps:
1) mixes according to the component of above-mentioned prescription 3;
2) above-mentioned freezing drying protective agent is sterilized, the temperature of sterilization is 100 ℃, and the time is 30 minutes.
The above-mentioned freezing drying protective agent for preparing is applied in the lactobacillus, lactobacillus is had preferable protection effect in freezing dry process.
Preparation has the lactobacillus fermentation agent of above-mentioned freezing drying protective agent: at first with centrifugal enrichment to lactobacillus bulgaricus join in the above-mentioned freezing drying protective agent in the ratio of mass ratio 1:1, under sterile state, both are mixed.Then the lactobacillus bacteria proliferated culture medium for preparing is stored in 4 ℃ of environment and stored 12 hours.
The lactobacillus freeze dried fermenting preparation that adopts above-mentioned cryoprotectant production is to carry out fermenting experiment in 11.5% sweet milk or the whole-milk under 43 ℃ condition with the dry matter content that is inoculated in sterilization in right amount; fermented 4.5-5 hour; titration acidity 〉=65 ° T, pH≤4.70, the sense organ local flavor is all good.According to 1:10(w/v) the fermentation using lactobacillus bulgaricus agent is dissolved in (121 ℃ of sterilizations, 15 minutes) virgin Chinese protein peptone water in, do suitable dilution then, cultivated 48-72 hour for last 37 ℃ at milk-acid bacteria substratum (MRS substratum), cell concentration is 4.3 * 10
9Cfu/g.
Lyophilized vaccine of the present invention has safety, cost is low and raw material is easy to obtain advantage.When adopting this protective material that lactobacillus is carried out lyophilize, can improve the survival rate of lactobacillus in refrigerating process, make the lactobacillus fermentation agent after the freeze-drying still can keep higher fermentative activity, therefore be more suitable for large-scale industrial production.
Above listed a series of detailed description only is specifying at feasibility embodiment of the present invention; they are not in order to restriction protection scope of the present invention, allly do not break away from equivalent embodiment or the change that skill spirit of the present invention done and all should be included within protection scope of the present invention.
Claims (4)
1. freezing drying protective agent is characterized in that 100 parts of described freezing drying protective agents are prepared from by the component of following weight part:
12 parts-20 parts of skimmed milk powders,
0.5 part-3 parts of Sodium Glutamates,
0.1 part-0.5 part of sodium ascorbate,
0.5 part-3 parts of glycerine,
0.05 part-0.3 part of Gelucystine,
10 parts-20 parts of trehaloses,
Other is supplied by water.
2. method for preparing freezing drying protective agent is characterized in that: the component described in the claim 1 is mixed, and is that 100 ℃ sterilization is prepared from through 30 minutes temperature.
3. freezing drying protective agent as claimed in claim 1 is used in microbial freezing drying.
4. application as claimed in claim 3, wherein, described microorganism is a lactobacillus.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559501A (en) * | 2012-01-16 | 2012-07-11 | 东南大学 | Freeze drying protective agent for bacillus pasteurii |
| CN107841463A (en) * | 2017-12-18 | 2018-03-27 | 江南大学 | A kind of high activity lactobacillus bacterium powder vacuum freezedrying production technology and application |
| WO2018081550A1 (en) * | 2016-10-28 | 2018-05-03 | Vedanta Biosciences, Inc. | Methods and compositions for preserving bacteria |
| CN108018317A (en) * | 2017-12-18 | 2018-05-11 | 江南大学 | A kind of method for improving Lactococcus lactis resistance and the application in bacterium powder preparation |
| CN110982762A (en) * | 2020-01-04 | 2020-04-10 | 广东环凯生物科技有限公司 | Clostridium sporogenes stabilizer and application thereof |
| CN113234712A (en) * | 2021-05-27 | 2021-08-10 | 浙江海洋大学 | Aerogel immobilized microorganism preservation method |
| WO2022141934A1 (en) * | 2020-12-30 | 2022-07-07 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Staphylococcus aureus standard strains containing specific molecular target, and detection and use thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1507878A (en) * | 2002-12-15 | 2004-06-30 | 东北农业大学 | Method for preparing lactobacillus dry-powder product |
| CN101486986A (en) * | 2009-02-12 | 2009-07-22 | 上海谱莱生物技术有限公司 | Preparation of freeze-dried Lactobacillus acidophilus powder |
-
2010
- 2010-05-28 CN CN 201010185602 patent/CN102260635B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1507878A (en) * | 2002-12-15 | 2004-06-30 | 东北农业大学 | Method for preparing lactobacillus dry-powder product |
| CN101486986A (en) * | 2009-02-12 | 2009-07-22 | 上海谱莱生物技术有限公司 | Preparation of freeze-dried Lactobacillus acidophilus powder |
Non-Patent Citations (1)
| Title |
|---|
| 《当代新型食品》 19981231 刘程 冷冻干燥工艺及对相关设备的要求 北京工业大学出版社 56 1-4 , * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559501A (en) * | 2012-01-16 | 2012-07-11 | 东南大学 | Freeze drying protective agent for bacillus pasteurii |
| WO2018081550A1 (en) * | 2016-10-28 | 2018-05-03 | Vedanta Biosciences, Inc. | Methods and compositions for preserving bacteria |
| US11447738B2 (en) | 2016-10-28 | 2022-09-20 | Vedanta Biosciences, Inc. | Methods and compositions for preserving bacteria |
| US11814615B2 (en) | 2016-10-28 | 2023-11-14 | Vedanta Biosciences, Inc. | Methods and compositions for preserving bacteria |
| CN107841463A (en) * | 2017-12-18 | 2018-03-27 | 江南大学 | A kind of high activity lactobacillus bacterium powder vacuum freezedrying production technology and application |
| CN108018317A (en) * | 2017-12-18 | 2018-05-11 | 江南大学 | A kind of method for improving Lactococcus lactis resistance and the application in bacterium powder preparation |
| CN110982762A (en) * | 2020-01-04 | 2020-04-10 | 广东环凯生物科技有限公司 | Clostridium sporogenes stabilizer and application thereof |
| WO2022141934A1 (en) * | 2020-12-30 | 2022-07-07 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Staphylococcus aureus standard strains containing specific molecular target, and detection and use thereof |
| CN113234712A (en) * | 2021-05-27 | 2021-08-10 | 浙江海洋大学 | Aerogel immobilized microorganism preservation method |
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