CN102258625A - Medicine for treating disease such as ulcerative colitis, colon cancer or rectal cancer and production method thereof - Google Patents
Medicine for treating disease such as ulcerative colitis, colon cancer or rectal cancer and production method thereof Download PDFInfo
- Publication number
- CN102258625A CN102258625A CN201010278360XA CN201010278360A CN102258625A CN 102258625 A CN102258625 A CN 102258625A CN 201010278360X A CN201010278360X A CN 201010278360XA CN 201010278360 A CN201010278360 A CN 201010278360A CN 102258625 A CN102258625 A CN 102258625A
- Authority
- CN
- China
- Prior art keywords
- extract
- radix glycyrrhizae
- fructus jujubae
- crude drug
- filtrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a medicine for treating the colonic disease, in particular to a medicine for treating the disease such as ulcerative colitis, colon cancer or rectal cancer. The medicine is prepared by comprising alhagi sparsifolia, liquorice, wolfberry, date extract and tea polyphenols. The medicine disclosed by the invention has the advantages of high efficiency, low toxin and no drug resistance, and has better curative effects on the colonic disease, especially on the ulcerative colitis, the colon cancer or the rectal cancer.
Description
Technical field
The present invention relates to a kind of medicine for the treatment of the colon disease, particularly a kind of medicine for the treatment of ulcerative colitis, colon cancer or rectal cancer one class disease
Background technology
At present for the ulcer colonic diseases, particularly lack effective treatment means for ulcerative colitis, colon cancer or rectal cancer, western medicine has efficient characteristics, but usually be accompanied by big toxic and side effects, also be easy to generate Drug resistance after taking medicine for a long time, and the treatment by Chinese herbs toxic and side effects is little, but also has the slower deficiency of produce effects.
Chinese patent 200910113204.5 provides a kind of isoflavone Lignanoids compounds that extracts from camel thorn plants, its structural formula is
Test shows that this chemical compound has antibacterial and anti-inflammation functions, has the low characteristics of toxic and side effects, but its further application is still indeterminate, and effect is also not obvious in clinical practice.
Chinese patent 200910113530.6 provides a kind of treatment allergic colitis medicine that extracts from camel thorn plants, this medicine is single prescription, be mainly used in the treatment allergic colitis, suitable and narrower, and be folk prescription for a certain reason, its curative effect is not fully exerted, has influenced result of use.
Therefore, a kind of efficient, low toxicity and be the pharmaceutical composition of main component with the extract from camel thorn plants, the pharmaceutical composition of particularly treating the colon disease is just removed to be proposed.
Summary of the invention
The pharmaceutical composition that the purpose of this invention is to provide a kind of treatment colon disease of efficient, low toxicity, particularly a kind of pharmaceutical composition for the treatment of ulcerative colitis, colon cancer or rectal cancer one class disease.
The objective of the invention is to be achieved through the following technical solutions:
It is with from Alhagi sparsifolia extract, and be equipped with Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae extract, tea polyphenols carries out prescription and forms, characteristics are that described proportioning is:
Camel thorn plants extract 1g, Radix Glycyrrhizae extract 3～10g, wolfberry fruit extract 8～15g, Fructus Jujubae extract 6～15g, tea polyphenols 0.2～1.0g.
Above-mentioned Radix Glycyrrhizae extract, wolfberry fruit extract and Fructus Jujubae extract are produced by the following method:
Alcohol extraction: use 40 ~ 95% ethanol to carry out reflux, extract, 1 ~ 2h respectively in Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae, cross 38 ~ 180 μ m sieve and get filtrate.
Above-mentioned reflux, extract, is preferably carried out more than twice, each 1 ~ 2h, and described each extraction press concentration of alcohol from high to low level time extract, Chinese crude drug and alcoholic acid mixing ratio are 0.05 ~ 0.2Kg/L when extracting at every turn.
Water is carried: alcohol extraction residue water is carried 1 ~ 2 time, and each 1 ~ 2h crosses 38 ~ 180 μ m sieve and gets filtrate, and the mixing ratio of Chinese crude drug and water is 0.05 ~ 0.2Kg/L during extraction.
Concentrate drying: said extracted gained filtrate concentrate drying to water content below 10%, is promptly got Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract.
Mix: above-mentioned Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract, tea polyphenols and camel thorn plants extract are mixed in proportion promptly obtain medicine of the present invention, can will add soluble starch or similar filler wet granulation in the mixture according to concrete needs, or make capsule, tablet.
Above-mentioned camel thorn plants extract can be produced by the following method:
Get exsiccant camel thorn plants aerial parts to pulverize or be cut into≤2 centimetres granule or section be as raw material.
Add the ratio reflux, extract, 1 ~ 2h of 10kg raw material in per 25000 ~ 35000ml, 50～70% ethanol, cross 38 ~ 180 μ m aperture sieve, get filtrate be concentrated into concentration of alcohol≤2% concentrated solution.
Above-mentioned extraction preferably repeats 2 ~ 5 times.
Extraction: concentrated solution is extracted 1 ~ 2h in concentrated solution volume 2-3 chloroform doubly, cross 38 ~ 180 μ m sieve, the gained extract is concentrated into chloroform concentration≤2%.
Silica gel column chromatography: measure chloroform extraction liquid through silica gel column chromatography by 8 ~ 12 times of silicagel column cylinder volumes.
Described silicagel column particle diameter is preferably below the 150 μ m.
Eluting: with content of acetone is petroleum ether-acetone mixed liquor eluting of 10～50%, collects eluent, through the following ODS high performance liquid chromatogram of 61 μ m preparative column, gained mobile phase is sloughed petroleum ether and acetone gets final product.
Camel thorn plants extract of the present invention also can be produced by the following method:
Get exsiccant camel thorn plants aerial parts, pulverize or be cut into≤2 centimetres granule or section be as raw material, the ethanol reflux, extract, that places 25000～40000ml 60～95% by every 10kg is more than 1 time, each more than 1 hour extracting solution stock solution.
With extracting solution be concentrated into dry matter content be not less than 5% extractum, be preferably 20～40%.
Extract more than 1 time to above-mentioned extractum:
A. the ethyl acetate of doubly measuring with concentrated gained extractum volume 1-3 extracts, and leaves standstill clarification in 4～6 hours to obvious layering occurring, goes ethyl acetate layer to keep water-soluble layer.
Preferably carry out at least a of following operation successively before a step: the 60-90 ℃ of petroleum ether of doubly measuring with extractum volume 1-3 extracts, and keeps water-soluble layer; Chloroform with 1～3 times of amount of volume extracts, and keeps water-soluble layer.
B. the n-butyl alcohol of doubly measuring with the water-soluble layer of a step volume 1-3 extracts, and leaves standstill clarification in 4～6 hours to obvious layering occurring, gets n-butanol layer solution, concentrates gained n-butanol layer extract and is medicine of the present invention.
Above-mentioned n-butanol layer solution is preferably in and is concentrated into dry matter content 〉=75% in the Rotary Evaporators.
Medicine of the present invention has efficiently, the advantage of low toxicity, no Drug resistance, to the colon disease, particularly ulcerative colitis, colon cancer, rectal cancer is had better curative effect.
The specific embodiment
Embodiment 1:
Get the dry former plant of camel thorn and be ground into 2 centimetres granule as raw material, ethanol by every 3500ml65% adds 10kg raw material reflux, extract, 1.5h, cross 180 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/3000ml65% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 2h in the chloroform of 2 times of concentrated solution volumes, cross 100 μ m sieve, trapped substance repeats 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measuring the silica gel column chromatography of chloroform extraction liquid through 150 μ m granularities by 8 times of silicagel column cylinder volumes, is petroleum ether-acetone mixed liquor eluting of 15% with content of acetone, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, immobile phase is a silica gel, and granularity 48 μ m slough petroleum ether with gained mobile phase and acetone obtains the camel thorn plants extract.
With the Chinese crude drug Radix Glycyrrhizae; Fructus Lycii and Fructus Jujubae are broken into 5mm following granule or powder respectively; the ratio 0.1Kg/L that presses Chinese crude drug and ethanol is extracting liquorice respectively; Fructus Lycii and big jujube granule or powder reflux, extract, three times in ethanol; extract used concentration of alcohol three times and be followed successively by 90%; 70% and 40%; each 2h; cross 180 μ m sieve after each the extraction and get filtrate; carry 1 time for 0.2Kg/L water by the mixing ratio of Chinese crude drug and water more afterwards; 2h; cross 180 μ m sieve and get filtrate; said extracted gained filtrate mixed concentrating after drying by Chinese crude drug kind difference respectively, obtain Radix Glycyrrhizae respectively to water content below 10%; Fructus Lycii; Fructus Jujubae extract.
The part by weight of pressing camel thorn plants extract 1g, Radix Glycyrrhizae extract 5g, wolfberry fruit extract 8g Fructus Jujubae extract 10g, tea polyphenols 0.2g mixes and adds soluble starch as the filler wet granulation, promptly obtains medicine of the present invention.
Embodiment 2:
Get the dry former plant of camel thorn and be ground into 1 centimetre granule as raw material, ethanol by every 3000ml 70% adds 10kg raw material reflux, extract, 2h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/3000ml65% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5h in the chloroform of 2 times of concentrated solution volumes, cross 74 μ m sieve, trapped substance repeats 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 124 μ m granularities, with content of acetone is petroleum ether-acetone mixed liquor eluting of 30%, collects eluent, through ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 25 μ m slough petroleum ether with gained mobile phase and acetone obtains camel thorn plants extract of the present invention.
With the Chinese crude drug Radix Glycyrrhizae; Fructus Lycii and Fructus Jujubae are broken into 3mm following granule or powder respectively; press Chinese crude drug and ethanol than 0.15Kg/L extracting liquorice; Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively; extract used concentration of alcohol three times and be followed successively by 95%; 70%; 50%; each 1.2h; cross 100 μ m sieve and get filtrate; carry 2 times for 0.2Kg/L water by the mixing ratio of Chinese crude drug and water again; each 1 ~ 2h; cross 100 μ m sieve and get filtrate; said extracted gained filtrate mixed concentrating after drying by Chinese crude drug kind difference respectively, obtain Radix Glycyrrhizae respectively to water content below 8%; Fructus Lycii; Fructus Jujubae extract.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 10g, wolfberry fruit extract 8g, Fructus Jujubae extract 6g, tea polyphenols 0.6g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 3:
Get the dry former plant of camel thorn and be ground into 0.5 centimetre granule as raw material, ethanol by every 3000ml 65% adds 10kg raw material reflux, extract, 2h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/2500ml70% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1 h in the chloroform of 2.5 times of concentrated solution volumes, cross 104 μ m sieve, trapped substance repeats 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measuring the silica gel column chromatography of chloroform extraction liquid through 124 μ m granularities by 10 times of silicagel column cylinder volumes, is petroleum ether-acetone mixed liquor eluting of 50% with content of acetone, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 15 μ m slough petroleum ether and acetone with gained mobile phase, obtain camel thorn plants extract of the present invention.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae are broken into the following granule of 4mm respectively, press Chinese crude drug and ethanol than 0.18Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be twice of reflux, extract, in ethanol respectively, the used concentration of alcohol of extracted twice is respectively 90% and 50%, each 2h, cross 106 μ m sieve and get filtrate, carry 2 times for 0.0.2Kg/L water by the mixing ratio of Chinese crude drug and water again, each 2h, cross 106 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 7% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively, Fructus Lycii, Fructus Jujubae extract.
The weight ratio of getting camel thorn plants extract 1g, Radix Glycyrrhizae extract 8g, wolfberry fruit extract 12g, Fructus Jujubae extract 6g, tea polyphenols 0.4g mix and add soluble starch as the filler wet granulation, promptly obtain medicine of the present invention.
Embodiment 4:
Get the dry former plant of camel thorn be cut into 1 centimetre with interior section as raw material, ethanol by every 3000ml 65% adds 10kg raw material reflux, extract, 1.5h, cross 74 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/2800ml65% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5 h in the chloroform of 2.5 times of concentrated solution volumes, cross 74 μ m sieve, trapped substance repeats 2 extractions by said ratio, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid through 74 μ m granularities by 12 times of silicagel column cylinder volumes, with pressing 8 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 25%, collect eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 23 μ m slough petroleum ether with gained mobile phase and acetone obtains camel thorn plants extract of the present invention.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 2mm respectively, press Chinese crude drug and ethanol than 0.2Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, four times in ethanol respectively, extract used concentration of alcohol four times and be followed successively by 95%, 70%, 55%, 40%, each 1.5h, cross 58 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water for 0.1Kg/L water again, cross 58 μ m sieve and get filtrate, said extracted gained filtrate mixed concentrating after drying by Chinese crude drug kind difference respectively, obtain Radix Glycyrrhizae respectively to water content below 10%, Fructus Lycii, Fructus Jujubae extract.
Mix and add soluble starch by the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 5g, wolfberry fruit extract 10g, Fructus Jujubae extract 10g, tea polyphenols 1.0g and make capsule, promptly obtain medicine of the present invention as filler.
Embodiment 5:
Get the dry former plant of camel thorn be cut into 0.5 centimetre with interior section as raw material, ethanol by every 3200ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 38 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/2800ml65% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5 h in the chloroform of 2.5 times of concentrated solution volumes, cross 38 μ m sieve, trapped substance repeats 1 extraction by said ratio, the gained extract is concentrated into chloroform concentration≤2%, measures the silica gel column chromatography of chloroform extraction liquid, with pressing 12 times of amounts of silicagel column cylinder volume through 104 μ m granularities by 9 times of silicagel column cylinder volumes, content of acetone is petroleum ether-acetone mixed liquor eluting of 45%, collect eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 13 μ m, gained mobile phase is sloughed petroleum ether and acetone, obtain camel thorn plants extract of the present invention.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 3mm respectively, press Chinese crude drug and ethanol than 0.15Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively, extract used concentration of alcohol three times and be respectively 95%, 75%, 55%, each 1.5h, cross 45 μ m sieve and get filtrate, carry 2 times for 0.1Kg/L water by the mixing ratio of Chinese crude drug and water again, each 1.5h, cross 45 μ m sieve and get filtrate, said extracted gained filtrate mixed concentrating after drying by Chinese crude drug kind difference respectively, obtain Radix Glycyrrhizae respectively to water content below 5%, Fructus Lycii, Fructus Jujubae extract.
Mix and add soluble starch by the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 8g, wolfberry fruit extract 8g, Fructus Jujubae extract 8g, tea polyphenols 0.5g and make capsule, promptly obtain medicine of the present invention as filler.
Embodiment 6:
Get the dry former plant of camel thorn and be ground into granule below 0.8 centimetre as raw material, ethanol by every 3400ml 55% adds 10kg raw material reflux, extract, 1.5h, cross 124 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in 10kg material quantity/2500ml 50% alcoholic acid ratio again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1 h in the chloroform of 2 times of concentrated solution volumes, cross 44 μ m sieve, trapped substance repeats 2 extractions by said ratio, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 11 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 8 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 30%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 18 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains camel thorn plants extract of the present invention.
Fructus Jujubae after Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and the enucleation is broken into the granule of 4mm respectively, press Chinese crude drug and ethanol than 0.12Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae twice of reflux, extract, in ethanol respectively, extract used concentration of alcohol and be respectively 90%, 55%, each 2h, cross 48 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.12Kg/L water again, cross 48 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 6% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract respectively.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 8g, wolfberry fruit extract 15g, Fructus Jujubae extract 15g, tea polyphenols 1.0g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 7:
Get the dry former plant of camel thorn and be ground into granule below 1.2 centimetres as raw material, ethanol by every 2500ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/2500ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5 h in the chloroform of 2 times of concentrated solution volumes, cross 61 μ m sieve, trapped substance is by extraction 1 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 25%, collect eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 10 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains camel thorn plants extract of the present invention.
Fructus Jujubae after Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and the enucleation is broken into the granule of 5mm respectively, press Chinese crude drug and ethanol than 0.08Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae twice of reflux, extract, in ethanol respectively, extract used concentration of alcohol and be respectively 85%, 55%, each 2h, cross 75 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.1Kg/L water again, cross 75 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 7% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract respectively.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 10g, wolfberry fruit extract 8g, Fructus Jujubae extract 6g, tea polyphenols 0.2g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 8:
Get the dry former plant of camel thorn and be ground into granule below 1.0 centimetres as raw material, ethanol by every 3300ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/2500ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.8 h in the chloroform of 2.2 times of concentrated solution volumes, cross 62 μ m sieve, trapped substance is by extraction 1 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 8 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 45%, collect eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 6.5 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains camel thorn plants extract of the present invention.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 5mm respectively, press Chinese crude drug and ethanol than 0.18Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively, extract used concentration of alcohol three times and be respectively 90%, 70%, 50%, each 1.5h, cross 150 μ m sieve and get filtrate, carry 1.5h by the mixing ratio of Chinese crude drug and water 1 time for 0.12Kg/L water again, cross 150 μ m sieve and get filtrate, said extracted gained filtrate mixed concentrating after drying by Chinese crude drug kind difference respectively, obtain Radix Glycyrrhizae respectively to water content below 10%, Fructus Lycii, Fructus Jujubae extract.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 6g, wolfberry fruit extract 15g, Fructus Jujubae extract 6g, tea polyphenols 0.7g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 9:
Get the dry former plant of camel thorn and be ground into granule below 0.8 centimetre as raw material, ethanol by every 3500ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/2800ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5 h in the chloroform of 2.8 times of concentrated solution volumes, cross 38 μ m sieve, trapped substance is by extraction 1.5 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 1 extraction, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 12 times of silicagel column cylinder volumes through 58 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 30%, collect eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 2.6 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains camel thorn plants extract of the present invention.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 4mm respectively, press Chinese crude drug and ethanol than 0.1Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, four times in ethanol respectively, extract used concentration of alcohol and be respectively 90%, 80%, 70%, 60%, each 1.2h, cross 180 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.1Kg/L water again, cross 180 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 9% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively, Fructus Lycii, Fructus Jujubae extract.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 9g, wolfberry fruit extract 12g, Fructus Jujubae extract 12g, tea polyphenols 0.5g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 10:
Get the dry former plant of camel thorn and be ground into granule below 0.5 centimetre as raw material, ethanol by every 3000ml 50% adds 10kg raw material reflux, extract, 2h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 1 time in the alcoholic acid ratio of 10kg material quantity/3000ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 2h in the chloroform of 2.5 times of concentrated solution volumes, cross 149 μ m sieve, trapped substance is by extraction 2 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 1 extraction, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 106 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 30%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 58 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains camel thorn plants extract of the present invention.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 3mm respectively, press Chinese crude drug and ethanol than 0.15Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively, extract used concentration of alcohol three times and be respectively 80%, 60%, 40%, each 1.5h, cross 120 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.1Kg/L water again, cross 120 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 7% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively, Fructus Lycii, Fructus Jujubae extract.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 4g, wolfberry fruit extract 8g, Fructus Jujubae extract 15g, tea polyphenols 1.0g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 11:
Get the dry former plant of camel thorn and be ground into granule below 0.6 centimetre as raw material, ethanol by every 3200ml 50% adds 10kg raw material reflux, extract, 1.2h, cross 124 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/2700ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 2h in the chloroform of 2.2 times of concentrated solution volumes, cross 89 μ m sieve, trapped substance is by extraction 2 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 1 extraction, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 45%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 74 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains camel thorn plants extract of the present invention.
With the Chinese crude drug Radix Glycyrrhizae; Fructus Jujubae after Fructus Lycii and the enucleation is broken into 5mm following granule or powder respectively; press Chinese crude drug and ethanol than 0.16Kg/L extracting liquorice; Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively; extract used concentration of alcohol three times and be respectively 90%; 75%; 50%; each 2h; cross 120 μ m sieve and get filtrate; carry 1.5h by the mixing ratio of Chinese crude drug and water 1 time for 0.2Kg/L water again; cross 120 μ m sieve and get filtrate; said extracted gained filtrate is mixed concentrated after drying respectively to water content 8% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively; Fructus Lycii; Fructus Jujubae extract.
Mix and add soluble starch by the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 5g, wolfberry fruit extract 12g, Fructus Jujubae extract 10g, tea polyphenols 0.8g and make tablet, promptly obtain medicine of the present invention as filler.
Embodiment 12:
Get the dry former plant of camel thorn and be ground into granule below 0.3 centimetre as raw material, ethanol by every 3300ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 124 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/2500ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 2h in the chloroform of 2.5 times of concentrated solution volumes, cross 44 μ m sieve, trapped substance is by extraction 1.5 h in the chloroform of 1.8 times of above-mentioned concentrated solution volumes and repeat 1 extraction, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 40%, collect eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 2.5 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains camel thorn plants extract of the present invention.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the powder of 1mm respectively, press Chinese crude drug and ethanol than 0.1Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively, extract used concentration of alcohol three times and be respectively 95%, 70%, 55%, each 1.2h, cross 38 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.05Kg/L water again, cross 38 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 5% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively, Fructus Lycii, Fructus Jujubae extract;
Mix and add soluble starch by the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 4g, wolfberry fruit extract 10g, Fructus Jujubae extract 6g, tea polyphenols 0.7g and make tablet, promptly obtain medicine of the present invention as filler.
Embodiment 13:
Get the dry former plant of camel thorn and be ground into 2 centimetres granule as raw material, ethanol by every 3500ml65% adds 10kg raw material reflux, extract, 1.5h, cross 180 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/3000ml65% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 2h in the chloroform of 2 times of concentrated solution volumes, cross 100 μ m sieve, trapped substance repeats 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measuring the silica gel column chromatography of chloroform extraction liquid through 150 μ m granularities by 8 times of silicagel column cylinder volumes, is petroleum ether-acetone mixed liquor eluting of 15% with content of acetone, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, immobile phase is a silica gel, and granularity 48 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the powder of 1mm respectively, press Chinese crude drug and ethanol than 0.1Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively, extract used concentration of alcohol three times and be respectively 95%, 70%, 55%, each 1.2h, cross 38 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.05Kg/L water again, cross 38 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 5% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively, Fructus Lycii, Fructus Jujubae extract;
Mix and add soluble starch by the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 4g, wolfberry fruit extract 10g, Fructus Jujubae extract 6g, tea polyphenols 0.7g and make tablet, promptly obtain medicine of the present invention as filler.
Embodiment 14:
Get the dry former plant of camel thorn and be ground into 1 centimetre granule as raw material, ethanol by every 3000ml 70% adds 10kg raw material reflux, extract, 2h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/3000ml65% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5h in the chloroform of 2 times of concentrated solution volumes, cross 74 μ m sieve, trapped substance repeats 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 124 μ m granularities, with content of acetone is petroleum ether-acetone mixed liquor eluting of 30%, collects eluent, through ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 25 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
With the Chinese crude drug Radix Glycyrrhizae; Fructus Jujubae after Fructus Lycii and the enucleation is broken into 5mm following granule or powder respectively; press Chinese crude drug and ethanol than 0.16Kg/L extracting liquorice; Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively; extract used concentration of alcohol three times and be respectively 90%; 75%; 50%; each 2h; cross 120 μ m sieve and get filtrate; carry 1.5h by the mixing ratio of Chinese crude drug and water 1 time for 0.2Kg/L water again; cross 120 μ m sieve and get filtrate; said extracted gained filtrate is mixed concentrated after drying respectively to water content 8% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively; Fructus Lycii; Fructus Jujubae extract.
Mix and add soluble starch by the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 5g, wolfberry fruit extract 12g, Fructus Jujubae extract 10g, tea polyphenols 0.8g and make tablet, promptly obtain medicine of the present invention as filler.
Embodiment 15:
Get the dry former plant of camel thorn and be ground into 0.5 centimetre granule as raw material, ethanol by every 3000ml 65% adds 10kg raw material reflux, extract, 2h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/2500ml70% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1 h in the chloroform of 2.5 times of concentrated solution volumes, cross 104 μ m sieve, trapped substance repeats 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 124 μ m granularities, with content of acetone is petroleum ether-acetone mixed liquor eluting of 50%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 15 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 4mm respectively, press Chinese crude drug and ethanol than 0.1Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, four times in ethanol respectively, extract used concentration of alcohol and be respectively 90%, 80%, 70%, 60%, each 1.2h, cross 180 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.1Kg/L water again, cross 180 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 9% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively, Fructus Lycii, Fructus Jujubae extract.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 9g, wolfberry fruit extract 12g, Fructus Jujubae extract 12g, tea polyphenols 0.5g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 16:
Get the dry former plant of camel thorn be cut into 1 centimetre with interior section as raw material, ethanol by every 3000ml 65% adds 10kg raw material reflux, extract, 1.5h, cross 74 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/2800ml65% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5 h in the chloroform of 2.5 times of concentrated solution volumes, cross 74 μ m sieve, trapped substance repeats 2 extractions by said ratio, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid through 74 μ m granularities by 12 times of silicagel column cylinder volumes, with pressing 8 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 25%, collect eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 23 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 3mm respectively, press Chinese crude drug and ethanol than 0.15Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively, extract used concentration of alcohol three times and be respectively 80%, 60%, 40%, each 1.5h, cross 120 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.1Kg/L water again, cross 120 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 7% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively, Fructus Lycii, Fructus Jujubae extract.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 4g, wolfberry fruit extract 8g, Fructus Jujubae extract 15g, tea polyphenols 1.0g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 17:
Get the dry former plant of camel thorn be cut into 0.5 centimetre with interior section as raw material, ethanol by every 3200ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 38 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/2800ml65% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5 h in the chloroform of 2.5 times of concentrated solution volumes, cross 38 μ m sieve, trapped substance repeats 1 extraction by said ratio, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid through 104 μ m granularities by 9 times of silicagel column cylinder volumes, with pressing 12 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 45%, collect eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 13 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 5mm respectively, press Chinese crude drug and ethanol than 0.18Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively, extract used concentration of alcohol three times and be respectively 90%, 70%, 50%, each 1.5h, cross 150 μ m sieve and get filtrate, carry 1.5h by the mixing ratio of Chinese crude drug and water 1 time for 0.12Kg/L water again, cross 150 μ m sieve and get filtrate, said extracted gained filtrate mixed concentrating after drying by Chinese crude drug kind difference respectively, obtain Radix Glycyrrhizae respectively to water content below 10%, Fructus Lycii, Fructus Jujubae extract.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 6g, wolfberry fruit extract 15g, Fructus Jujubae extract 6g, tea polyphenols 0.7g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 18:
Get the dry former plant of camel thorn and be ground into granule below 0.8 centimetre as raw material, ethanol by every 3400ml 55% adds 10kg raw material reflux, extract, 1.5h, cross 124 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in 10kg material quantity/2500ml 50% alcoholic acid ratio again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1 h in the chloroform of 2 times of concentrated solution volumes, cross 44 μ m sieve, trapped substance repeats 2 extractions by said ratio, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 11 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 8 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 30%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 18 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
Embodiment 19:
Get the dry former plant of camel thorn and be ground into granule below 1.2 centimetres as raw material, ethanol by every 2500ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/2500ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5 h in the chloroform of 2 times of concentrated solution volumes, cross 61 μ m sieve, trapped substance is by extraction 1 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 25%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 10 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 3mm respectively, press Chinese crude drug and ethanol than 0.15Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, three times in ethanol respectively, extract used concentration of alcohol three times and be respectively 95%, 75%, 55%, each 1.5h, cross 45 μ m sieve and get filtrate, carry 2 times for 0.1Kg/L water by the mixing ratio of Chinese crude drug and water again, each 1.5h, cross 45 μ m sieve and get filtrate, said extracted gained filtrate mixed concentrating after drying by Chinese crude drug kind difference respectively, obtain Radix Glycyrrhizae respectively to water content below 5%, Fructus Lycii, Fructus Jujubae extract.
Mix and add soluble starch by the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 8g, wolfberry fruit extract 8g, Fructus Jujubae extract 8g, tea polyphenols 0.5g and make capsule, promptly obtain medicine of the present invention as filler.
Embodiment 20:
Get the dry former plant of camel thorn and be ground into granule below 1.0 centimetres as raw material, ethanol by every 3300ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 3 times in the alcoholic acid ratio of 10kg material quantity/2500ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.8 h in the chloroform of 2.2 times of concentrated solution volumes, cross 62 μ m sieve, trapped substance is by extraction 1 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 2 extractions, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 8 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 45%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 6.5 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
Fructus Jujubae after Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and the enucleation is broken into the granule of 5mm respectively, press Chinese crude drug and ethanol than 0.08Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae twice of reflux, extract, in ethanol respectively, extract used concentration of alcohol and be respectively 85%, 55%, each 2h, cross 75 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.1Kg/L water again, cross 75 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 7% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract respectively.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 10g, wolfberry fruit extract 8g, Fructus Jujubae extract 6g, tea polyphenols 0.2g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 21:
Get the dry former plant of camel thorn and be ground into granule below 0.8 centimetre as raw material, ethanol by every 3500ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/2800ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 1.5 h in the chloroform of 2.8 times of concentrated solution volumes, cross 38 μ m sieve, trapped substance is by extraction 1.5 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 1 extraction, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 12 times of silicagel column cylinder volumes through 58 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 30%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 2.6 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Jujubae after Fructus Lycii and the enucleation is broken into the granule of 2mm respectively, press Chinese crude drug and ethanol than 0.2Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be reflux, extract, four times in ethanol respectively, extract used concentration of alcohol four times and be followed successively by 95%, 70%, 55%, 40%, each 1.5h, cross 58 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water for 0.1Kg/L water again, cross 58 μ m sieve and get filtrate, said extracted gained filtrate mixed concentrating after drying by Chinese crude drug kind difference respectively, obtain Radix Glycyrrhizae respectively to water content below 10%, Fructus Lycii, Fructus Jujubae extract.
Mix and add soluble starch by the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 5g, wolfberry fruit extract 10g, Fructus Jujubae extract 10g, tea polyphenols 1.0g and make capsule, promptly obtain medicine of the present invention as filler.
Embodiment 22:
Get the dry former plant of camel thorn and be ground into granule below 0.5 centimetre as raw material, ethanol by every 3000ml 50% adds 10kg raw material reflux, extract, 2h, cross 150 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 1 time in the alcoholic acid ratio of 10kg material quantity/3000ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 2h in the chloroform of 2.5 times of concentrated solution volumes, cross 149 μ m sieve, trapped substance is by extraction 2 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 1 extraction, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 106 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 30%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 58 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
Fructus Jujubae after Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and the enucleation is broken into the granule of 4mm respectively, press Chinese crude drug and ethanol than 0.12Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae twice of reflux, extract, in ethanol respectively, extract used concentration of alcohol and be respectively 90%, 55%, each 2h, cross 48 μ m sieve and get filtrate, carry 2h by the mixing ratio of Chinese crude drug and water 1 time for 0.12Kg/L water again, cross 48 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 6% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract respectively.
Press the weight ratio of camel thorn plants extract 1g, Radix Glycyrrhizae extract 8g, wolfberry fruit extract 15g, Fructus Jujubae extract 15g, tea polyphenols 1.0g and mix and add soluble starch, promptly obtain medicine of the present invention as the filler wet granulation.
Embodiment 23:
Get the dry former plant of camel thorn and be ground into granule below 0.6 centimetre as raw material, ethanol by every 3200ml 50% adds 10kg raw material reflux, extract, 1.2h, cross 124 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/2700ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 2h in the chloroform of 2.2 times of concentrated solution volumes, cross 89 μ m sieve, trapped substance is by extraction 2 h in the chloroform of 1.5 times of above-mentioned concentrated solution volumes and repeat 1 extraction, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 45%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 74 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
With the Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae are broken into the following granule of 4mm respectively, press Chinese crude drug and ethanol than 0.18Kg/L extracting liquorice, Fructus Lycii or Fructus Jujubae be twice of reflux, extract, in ethanol respectively, the used concentration of alcohol of extracted twice is respectively 90% and 50%, each 2h, cross 106 μ m sieve and get filtrate, carry 2 times for 0.0.2Kg/L water by the mixing ratio of Chinese crude drug and water again, each 2h, cross 106 μ m sieve and get filtrate, said extracted gained filtrate is mixed concentrated after drying respectively to water content 7% by Chinese crude drug kind difference, obtain Radix Glycyrrhizae respectively, Fructus Lycii, Fructus Jujubae extract.
The weight ratio of getting camel thorn plants extract 1g, Radix Glycyrrhizae extract 8g, wolfberry fruit extract 12g, Fructus Jujubae extract 6g, tea polyphenols 0.4g mix and add soluble starch as the filler wet granulation, promptly obtain medicine of the present invention.
Embodiment 24:
Get the dry former plant of camel thorn and be ground into granule below 0.3 centimetre as raw material, ethanol by every 3300ml 50% adds 10kg raw material reflux, extract, 1.5h, cross 124 μ m aperture sieve, get filtrate, trapped substance repeats reflux, extract, 2 times in the alcoholic acid ratio of 10kg material quantity/2500ml50% again, merging filtrate, concentrating under reduced pressure and dealcoholysis to concentration of alcohol≤2% gets concentrated solution, concentrated solution is extracted 2h in the chloroform of 2.5 times of concentrated solution volumes, cross 44 μ m sieve, trapped substance is by extraction 1.5 h in the chloroform of 1.8 times of above-mentioned concentrated solution volumes and repeat 1 extraction, the gained extract is concentrated into chloroform concentration≤2%, measure the silica gel column chromatography of chloroform extraction liquid by 10 times of silicagel column cylinder volumes through 74 μ m granularities, with pressing 10 times of amounts of silicagel column cylinder volume, content of acetone is petroleum ether-acetone mixed liquor eluting of 40%, collects eluent, through anti-phase ODS high performance liquid chromatogram preparative column, stationary-phase particle size degree 2.5 μ m slough petroleum ether with gained mobile phase and acetone promptly obtains the camel thorn plants extract.
With the Chinese crude drug Radix Glycyrrhizae; Fructus Lycii and Fructus Jujubae are broken into 5mm following granule or powder respectively; the ratio 0.1Kg/L that presses Chinese crude drug and ethanol is extracting liquorice respectively; Fructus Lycii and big jujube granule or powder reflux, extract, three times in ethanol; extract used concentration of alcohol three times and be followed successively by 90%; 70% and 40%; each 2h; cross 180 μ m sieve after each the extraction and get filtrate; carry 1 time for 0.2Kg/L water by the mixing ratio of Chinese crude drug and water more afterwards; 2h; cross 180 μ m sieve and get filtrate; said extracted gained filtrate mixed concentrating after drying by Chinese crude drug kind difference respectively, obtain Radix Glycyrrhizae respectively to water content below 10%; Fructus Lycii; Fructus Jujubae extract.
The part by weight of pressing camel thorn plants extract 1g, Radix Glycyrrhizae extract 5g, wolfberry fruit extract 8g Fructus Jujubae extract 10g, tea polyphenols 0.2g mixes and adds soluble starch as the filler wet granulation, promptly obtains medicine of the present invention.
Claims (5)
1. medicine for the treatment of ulcerative colitis, colon cancer or rectal cancer one class disease, it be with Alhagi sparsifolia extract and be equipped with Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae extract, tea polyphenols carries out prescription and forms, and is characterised in that described proportioning is:
Camel thorn plants extract 1g, Radix Glycyrrhizae extract 3～10g, wolfberry fruit extract 8～15g, Fructus Jujubae extract 6～15g, tea polyphenols 0.2～1.0g;
Described Radix Glycyrrhizae extract, wolfberry fruit extract and Fructus Jujubae extract are produced by the following method:
Alcohol extraction: use 40 ~ 95% ethanol to carry out reflux, extract, at least twice respectively in Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae, each 1 ~ 2h, cross 38 ~ 180 μ m sieve and get filtrate, and described extracted twice is at least pressed concentration of alcohol level time extraction from high to low, and Chinese crude drug and alcoholic acid mixing ratio are 0.05 ~ 0.2Kg/L when extracting at every turn;
Water is carried: alcohol extraction residue water is carried 1 ~ 2 time, and each 1 ~ 2h crosses 38 ~ 180 μ m sieve and gets filtrate, and the mixing ratio of Chinese crude drug and water is 0.05 ~ 0.2Kg/L when extracting at every turn;
Concentrate drying: with said extracted gained filtrate concentrate drying to water content below 10%, Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract;
Mix: above-mentioned Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract, tea polyphenols and camel thorn plants extract are mixed in proportion.
2. the medicine of treatment ulcerative colitis according to claim 1, colon cancer or rectal cancer one class disease is characterized in that described Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae are broken into 5mm following granule or powder.
3. medicine production method for the treatment of ulcerative colitis, colon cancer or rectal cancer one class disease, it be with Alhagi sparsifolia extract and be equipped with Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae extract, tea polyphenols carries out prescription and forms, be characterised in that:
Described Radix Glycyrrhizae extract, wolfberry fruit extract and Fructus Jujubae extract are produced by the following method:
Alcohol extraction: use 40 ~ 95% ethanol to carry out reflux, extract, 1 ~ 2h respectively in Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae, cross 38 ~ 180 μ m sieve and get filtrate;
Water is carried: alcohol extraction residue water is carried 1 ~ 2 time, and each 1 ~ 2h crosses 38 ~ 180 μ m sieve and gets filtrate, and the mixing ratio of Chinese crude drug and water is 0.05 ~ 0.2Kg/L during extraction;
Concentrate drying: with said extracted gained filtrate concentrate drying to water content below 10%, Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract;
Mix: above-mentioned Radix Glycyrrhizae, Fructus Lycii, Fructus Jujubae extract, tea polyphenols and camel thorn plants extract are mixed in proportion.
4. the medicine production method of treatment ulcerative colitis according to claim 3, colon cancer or rectal cancer one class disease is characterized in that described Chinese crude drug Radix Glycyrrhizae, Fructus Lycii and Fructus Jujubae are broken into 5mm following granule or powder.
5. according to the medicine production method of claim 3 or 4 described treatment ulcerative colitiss, colon cancer or rectal cancer one class disease, it is characterized in that:
In the extraction of described Radix Glycyrrhizae extract, wolfberry fruit extract and Fructus Jujubae extract, the reflux, extract, of described alcohol extraction is carried out more than twice, each 1 ~ 2h, and described each extraction press concentration of alcohol from high to low level time extract, Chinese crude drug and alcoholic acid mixing ratio are 0.05 ~ 0.2Kg/L when extracting at every turn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010278360XA CN102258625A (en) | 2010-05-24 | 2010-09-11 | Medicine for treating disease such as ulcerative colitis, colon cancer or rectal cancer and production method thereof |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010180975.9 | 2010-05-24 | ||
| CN201010180975 | 2010-05-24 | ||
| CN201010278360XA CN102258625A (en) | 2010-05-24 | 2010-09-11 | Medicine for treating disease such as ulcerative colitis, colon cancer or rectal cancer and production method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102258625A true CN102258625A (en) | 2011-11-30 |
Family
ID=45005591
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010278360XA Pending CN102258625A (en) | 2010-05-24 | 2010-09-11 | Medicine for treating disease such as ulcerative colitis, colon cancer or rectal cancer and production method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102258625A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITRM20130542A1 (en) * | 2013-10-04 | 2015-04-05 | Aboca Spa Societa Agricola | NEW FRACTIONS OF LIQUORICE EXTRACTS AND THEIR USES |
| CN114767716A (en) * | 2022-04-24 | 2022-07-22 | 华南农业大学 | Kefir whey and tea polyphenol composition as well as preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101259159A (en) * | 2008-04-17 | 2008-09-10 | 中国科学院新疆理化技术研究所 | Preparation of alhagi sparsifolia total flavones and uses thereof |
-
2010
- 2010-09-11 CN CN201010278360XA patent/CN102258625A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101259159A (en) * | 2008-04-17 | 2008-09-10 | 中国科学院新疆理化技术研究所 | Preparation of alhagi sparsifolia total flavones and uses thereof |
Non-Patent Citations (6)
| Title |
|---|
| > 20080430 张贵杰等 骆驼刺属植物的化学成分与生物活性 157-160 1-5 第23卷, 第4期 * |
| > 20090531 贾晓光等 维药骆驼刺提取物药理活性研究 18-20 1-5 第27卷, 第5期 * |
| > 20090630 张贵杰等 骆驼刺的化学成分研究 897-899 1-5 第44卷, 第12期 * |
| 张贵杰等: "骆驼刺属植物的化学成分与生物活性", <<国外医药-植物药分册>> * |
| 张贵杰等: "骆驼刺的化学成分研究", <<中国药学杂志>> * |
| 贾晓光等: "维药骆驼刺提取物药理活性研究", <<新疆中医药>> * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITRM20130542A1 (en) * | 2013-10-04 | 2015-04-05 | Aboca Spa Societa Agricola | NEW FRACTIONS OF LIQUORICE EXTRACTS AND THEIR USES |
| EP2857068A1 (en) * | 2013-10-04 | 2015-04-08 | Aboca S.p.A. Societa' Agricola | Fractions of liquorice extracts depleted of glycyrrhizic acid and uses thereof |
| CN114767716A (en) * | 2022-04-24 | 2022-07-22 | 华南农业大学 | Kefir whey and tea polyphenol composition as well as preparation method and application thereof |
| CN114767716B (en) * | 2022-04-24 | 2024-03-26 | 华南农业大学 | Kefir whey and tea polyphenol composition and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109438241A (en) | A kind of industrial method of synchronous preparation STEVIA REBAUDIANA chlorogenic acid and steviol glycoside | |
| CN105503786B (en) | Split Lignanoids compounds-smartweed diffractive ring lignan glucoside E and preparation method thereof | |
| CN108186474A (en) | Loranthus plant Loranthus tanakae extractive of general flavone, preparation method and application | |
| CN101538296B (en) | Active ingredients of camptosorus sibiricus, and extraction method and use of same | |
| CN105130759A (en) | Extraction method of magnolia officinalis extract | |
| CN102731592A (en) | Method for extracting cleupin and amentoflavone from olive leaf | |
| CN102258625A (en) | Medicine for treating disease such as ulcerative colitis, colon cancer or rectal cancer and production method thereof | |
| CN102716255B (en) | Eucommia ulmoides medicinal wine and preparation method thereof | |
| CN105106252A (en) | Method for extracting and separating flavonoid compounds from hypericum japonicum | |
| CN102503996B (en) | Method for extracting active constituent from Swertia mussotii plant | |
| CN102225956A (en) | Method for extracting and separating poliumoside from Gallicarpa kwangtungensis Chun | |
| CN100542523C (en) | Capsule with hairy vein agrimony and arenobufagin and preparation method thereof, method of quality control | |
| CN103497091B (en) | Compound with tyrosinase inhibitory activity and preparation method thereof | |
| CN101628030A (en) | Method for preparing folium orthosiphoni extractive and composition thereof | |
| CN105646638B (en) | The preparation method of pedunculoside | |
| CN105012378A (en) | Licorice root formula particle preparation method | |
| CN102040635A (en) | Method for efficiently separating and purifying forsythiaside B monomer and poliumoside monomer | |
| CN102247436B (en) | Preparation method of Qingxuanpian | |
| CN101703554A (en) | Preparation and use of semen cuscutae flavonoids | |
| CN110003290B (en) | Mechanochemical extraction method for preparing hydrolysable tannin crude extract from water chestnut shells | |
| CN109553654B (en) | The method of glycyrrhizin, licoflavone and licorice polysaccharide is extracted from Radix Glycyrrhizae | |
| CN114106008A (en) | Diterpene quinone compound extracted and separated from salvia miltiorrhiza bunge and method and application thereof | |
| CN101940615A (en) | New preparation method of Isodon excisa total diterpene | |
| CN101875685A (en) | Lysimachia foenum-graecum hance saponin extract having more than or equal to 30 percent of saponin and extraction process thereof | |
| Kumar et al. | Green and proficient process for industrial-scale preparation of Gymnema sylvestre standardized-extract enriched with Gymnemic acids through polymer-matrix-adsorption to reduce hyperglycemia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20111130 |