CN102253229A - High-flux dry biochemical chip - Google Patents
High-flux dry biochemical chip Download PDFInfo
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- CN102253229A CN102253229A CN201110161162XA CN201110161162A CN102253229A CN 102253229 A CN102253229 A CN 102253229A CN 201110161162X A CN201110161162X A CN 201110161162XA CN 201110161162 A CN201110161162 A CN 201110161162A CN 102253229 A CN102253229 A CN 102253229A
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Abstract
The invention relates to a high-flux dry biochemical chip which comprises a chip base, a filter layer and a reaction hole cover board, wherein a plurality of mutually independent reaction holes are formed on the chip base; a reagent layer and a color development layer are arranged in each reaction hole from top to bottom; sample injection holes are formed on the reaction hole cover board; the reaction hole cover board is arranged on the chip base and the sample injection holes are positioned on the reaction holes; and the filter layer is positioned between the sample injection holes and the reaction holes. Preferably, the chip base is provided with a concave part; the reaction holes are formed in the concave part; the reaction hole cover board is arranged in the concave part; the chip base is a plastic base, a cylindrical base or a transparent base; the number of reaction holes is more than 10; the sample injection holes are distributed on an inner circle and an outer circle; and the filter layer is a filter membrane. The high-flux dry biochemical chip is unique in design and ingenious in idea, so that the detection efficiency can be improved, the using amount of sample is reduced and the cost is reduced; and the high-flux dry biochemical chip is suitable for large-scale popularization and application.
Description
Technical field
The present invention relates to the biochemical chip technical field, particularly dry type biochemical chip technical field specifically is meant a kind of high flux dry type biochemical chip, is used for the dry type biochemistry detection field of clinical inspection analytic instrument and diagnostic reagent.
Background technology
Biochemical chip is a kind of as dry type biochemistry, mainly be in conjunction with supporting instrument sample to be carried out qualitative or quantitative test for medical institutions, the biochemistry detection mode of Cai Yonging mainly adopts the liquid phase turbidimetry in the market, the necessary instrument complexity, operating personnel are had relatively high expectations, the reagent storage life is shorter, and uncork stability is bad, and the sample size that needs is bigger; Each sample need add reagent separately and detect, and the amount of expending of reagent is big, and speed limit has bottleneck, and forms cross pollution easily in the course of reaction.
Therefore,, thereby can improve detection efficiency, reduce sample and detectable consumption, reduce cost, reduce cross pollution for needs provide a kind of high flux dry type biochemical chip.
Summary of the invention
In order to solve the problem of above-mentioned existence, the purpose of this invention is to provide a kind of high flux dry type biochemical chip, this high flux dry type biochemical chip design is unique, be skillfully constructed, thereby can improve detection efficiency, reduce sample and detectable consumption, reduce cost, reduce cross pollution, be suitable for large-scale promotion application.
To achieve these goals, high flux dry type biochemical chip of the present invention, be characterized in, comprise chip base, filtering layer and reacting hole cover plate, described chip base is provided with separate some reacting holes, be disposed with reagent layer and color layer in the described reacting hole from top to bottom, described reacting hole cover plate offers well, described reacting hole cover plate lid is located on the described chip base and described well is positioned on the described reacting hole, and described filtering layer is between described well and described reacting hole.
The well of described reacting hole cover plate plays the effect of diffusion layer, and detected sample infiltrates from well, thereby is used for detected sample by being evenly distributed to filtering layer.
Thereby described filtering layer is used for detected sample by removing chaff interference.
At described reagent layer, the reagent in detected sample and the reagent layer reacts; Reagent layer is generally made with freeze-dried powder reagent, and reagent generally is with the reaction reagent of dry type biochemical reaction for example Mg ion and AMP mix reagent.
Reaction product reaches color layer, thereby presents different colors, and color layer is generally made with the freeze-dried powder developer, and developer is the reagent para-nitro-pheneye phosphate for example that makes reaction product colour developing.
Described chip base can be any suitable shape, and preferably, described chip base has recess, and described reacting hole is arranged in the described recess, and described reacting hole cover plate lid is located in the described recess.
Described reacting hole can be scattered in any suitable shape, and more preferably, described reacting hole is circle distribution.
Described chip base can be any suitable solid material.Preferably, described chip base is a plastic base.
Described chip base can be any suitable shape, and preferably, described chip base is the right cylinder pedestal.
Described chip base can be transparent also can be opaque, preferably, described chip base is a transparent base.Thereby can observe the color that each hole reaction is presented from each bottom, hole, the back side of chip base.
The number of described reacting hole can be determined as required.Preferably, the number of described reacting hole is greater than 10.
Described well can be scattered in any suitable shape, and preferably, described well is inside and outside two circle circles and distributes.
Described filtering layer can adopt any suitable components, and preferably, described filtering layer is a filtering membrane.
Beneficial effect of the present invention is: high flux dry type biochemical chip of the present invention comprises chip base, filtering layer and reacting hole cover plate, chip base is provided with separate some reacting holes, be disposed with reagent layer and color layer in the reacting hole from top to bottom, the reacting hole cover plate offers well, reacting hole cover plate lid is located on the chip base and well is positioned on the reacting hole, filtering layer is between well and reacting hole, therefore, can high throughput testing, speed significantly improves with respect to present method; Required detection sample size reduces significantly; Can detect a plurality of projects simultaneously; Each reacts separate, and the centre does not produce cross pollution; Design is unique, is skillfully constructed, thereby can improves detection efficiency, reduces sample and detectable consumption, reduces cost, and reduces cross pollution, is suitable for large-scale promotion application.
Description of drawings
Fig. 1 is a schematic perspective view of the chip base of a specific embodiment of the present invention.
Fig. 2 is another schematic perspective view of chip base shown in Figure 1.
Fig. 3 is the schematic perspective view of the reacting hole cover plate of a specific embodiment of the present invention.
Embodiment
In order more to be expressly understood technology contents of the present invention, the spy enumerates following specific embodiment and describes in detail.Yet specific embodiment is only used for illustrating, rather than limitation of the present invention.
The structure of the high flux dry type biochemical chip of embodiment 1-3 all as Figure 1-3, high flux dry type biochemical chip comprises chip base 1, filtering layer (not shown) and reacting hole cover plate 2, chip base 1 is provided with 20 separate reacting holes 11, be disposed with reagent layer (not shown) and color layer (not shown) in the reacting hole 11 from top to bottom, reacting hole cover plate 2 offers well 21, chip base 1 has recess 12, reacting hole 11 is arranged in the recess 12, reacting hole cover plate 2 lids are located in the recess 12, well 21 is positioned on the reacting hole 11, well 21 is inside and outside two circle circles and distributes, and is crisscross arranged, filtering layer is a filtering membrane, between well 21 and reacting hole 11, all inject a certain amount of dry type biochemical reagents in each reacting hole 11, the biochemical reaction between each reacting hole 11 all is independently, do not disturb mutually.Thereby whole high flux dry type biochemical chip forms a plurality of functional layers from top to bottom successively: diffusion layer, filtering layer, reagent layer, color layer.Difference as detailed below.
Embodiment 1
Ground floor: diffusion layer, impel sample evenly to spread.
The second layer: filtering layer, remove chaff interference, adopt the nitrocellulose membrane material to make.
The 3rd layer: reagent layer, sample and reagent are reacted, adopt freeze-dried powder reagent to make, reagent is Mg ion and AMP mix reagent.
The 4th layer: color layer, make the end reaction thing present different colours, adopt the freeze-dried powder developer to make, developer is a para-nitro-pheneye phosphate.
Detected sample infiltrates by the well on the ground floor reacting hole cover plate 2 21 during reaction, enter after filtering through second layer filtering membrane in the reacting hole 11 in the chip base 1 with the 3rd layer reagent and react, continue infiltration downwards and the 4th layer chromogenic reagent generation chromogenic reaction then until end, reaction finishes the back and reads the change color value in each hole by the necessary instrument unification, and with instrument in the typical curve contrast that prestores obtain the concentration of each test event in the sample.
The test process of this case description alkaline phosphatase project, alkaline phosphatase in the sample is under alkaline ph value, but the hydrolysis of catalysis para-nitro-pheneye phosphate generates p-nitrophenol, p-nitrophenol has bigger absorption at the 400nm place, by instrument reflected light is carried out spectroscopic assay, and be translated into the activity degree of enzyme.
Embodiment 2
Ground floor: diffusion layer, impel sample evenly to spread.
The second layer: filtering layer, remove chaff interference, adopt the nitrocellulose membrane material to make.
The 3rd layer: reagent layer, sample and reagent are reacted, adopt freeze-dried powder reagent to make, reagent is glycylglycine.
The 4th layer: color layer, make the end reaction thing present different colours, adopt the freeze-dried powder developer to make, developer is a γ glutamy p-nitrophenyl ammonia.
Detected sample infiltrates by the well on the ground floor reacting hole cover plate 2 21 during reaction, enter after filtering through second layer filtering membrane in the reacting hole 11 in the chip base 1 with the 3rd layer reagent and react, continue infiltration downwards and the 4th layer chromogenic reagent generation chromogenic reaction then until end, reaction finishes the back and reads the change color value in each hole by the necessary instrument unification, and with instrument in the typical curve contrast that prestores obtain the concentration of each test event in the sample.
This item description the test process of gamma glutamyl transpeptidase, with patient's sample drop on chip, sample is distributed uniformly and is penetrated into reagent layer and color layer through behind the filtering layer, paddy amine acyl part in gamma glutamyl transpeptidase in the sample (GGT) the catalysis gamma-glutamyl paranitroanilinum shifts to two sweet amine peptides, and produce paranitrobenzoic acid simultaneously, carry out spectroscopic assay and calculate GGT activity value in the sample by instrument.
Embodiment 3
Ground floor: diffusion layer, impel sample evenly to spread.
The second layer: filtering layer, remove chaff interference, adopt the nitrocellulose membrane material to make.
The 3rd layer: reagent layer, sample and reagent are reacted, adopt freeze-dried powder reagent to make, reagent is LiOH dry type reagent.
The 4th layer: color layer, make the end reaction thing present different colours, adopt the freeze-dried powder developer to make, developer is a cupric tartrate.
Detected sample infiltrates by the well on the ground floor reacting hole cover plate 2 21 during reaction, enter after filtering through second layer filtering membrane in the reacting hole 11 in the chip base 1 with the 3rd layer reagent and react, continue infiltration downwards and the 4th layer chromogenic reagent generation chromogenic reaction then until end, reaction finishes the back and reads the change color value in each hole by the necessary instrument unification, and with instrument in the typical curve contrast that prestores obtain the concentration of each test event in the sample.
This case description the test process of total protein project, with patient's sample drop on dry plate, sample reacts in reagent layer after by filtering layer, be penetrated into color layer again and developer reacts, generate coloured complex, carry out reflection spectrum measuring by instrument and obtain Protein content in the sample.
Adopt high flux dry type biochemical chip of the present invention, chip base 1 adopts solid material, beat some reacting holes 11 on it, all put into dry type biochemical reagents material in each reacting hole 11, the biochemical reaction between each reacting hole 11 all is independently, does not disturb mutually, but every high flux dry type biochemical chip test item＞10, high throughput testing, speed is fast, and patient samples is few, and cost is low.
To sum up, high flux dry type biochemical chip design of the present invention is unique, is skillfully constructed, thereby can improves detection efficiency, reduces sample and detectable consumption, reduces cost, and reduces cross pollution, is suitable for large-scale promotion application.
In this instructions, the present invention is described with reference to its certain embodiments.But, still can make various modifications and conversion obviously and not deviate from the spirit and scope of the present invention.Therefore, instructions and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (9)
1. high flux dry type biochemical chip, it is characterized in that, comprise chip base, filtering layer and reacting hole cover plate, described chip base is provided with separate some reacting holes, be disposed with reagent layer and color layer in the described reacting hole from top to bottom, described reacting hole cover plate offers well, and described reacting hole cover plate lid is located on the described chip base and described well is positioned on the described reacting hole, and described filtering layer is between described well and described reacting hole.
2. high flux dry type biochemical chip according to claim 1 is characterized in that described chip base has recess, and described reacting hole is arranged in the described recess, and described reacting hole cover plate lid is located in the described recess.
3. high flux dry type biochemical chip according to claim 2 is characterized in that described reacting hole is circle distribution.
4. high flux dry type biochemical chip according to claim 1 is characterized in that described chip base is a plastic base.
5. high flux dry type biochemical chip according to claim 1 is characterized in that described chip base is the right cylinder pedestal.
6. high flux dry type biochemical chip according to claim 1 is characterized in that described chip base is a transparent base.
7. high flux dry type biochemical chip according to claim 1 is characterized in that the number of described reacting hole is greater than 10.
8. high flux dry type biochemical chip according to claim 1 is characterized in that, described well is inside and outside two circle circles and distributes.
9. high flux dry type biochemical chip according to claim 1 is characterized in that described filtering layer is a filtering membrane.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110161162XA CN102253229A (en) | 2011-06-15 | 2011-06-15 | High-flux dry biochemical chip |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110161162XA CN102253229A (en) | 2011-06-15 | 2011-06-15 | High-flux dry biochemical chip |
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| CN102253229A true CN102253229A (en) | 2011-11-23 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111426823A (en) * | 2020-04-30 | 2020-07-17 | 益善生物技术股份有限公司 | Reaction cassette for detection and detection operation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5443988A (en) * | 1992-09-02 | 1995-08-22 | Fuji Photo Film Co., Ltd. | Method for high speed analysis of a dry analytical element |
| CN1639557A (en) * | 2001-04-11 | 2005-07-13 | 伯斯坦技术公司 | Multi-parameter assays including analysis discs and methods relating thereto |
| CN1985174A (en) * | 2004-07-12 | 2007-06-20 | 爱科来株式会社 | Analyzer, method for specifying reaction vessel in analyzer, and analytical apparatus |
| CN101598727A (en) * | 2009-07-09 | 2009-12-09 | 上海科华生物工程股份有限公司 | The drying chemical reagent paper of quantitatively determining human blood urea content |
-
2011
- 2011-06-15 CN CN201110161162XA patent/CN102253229A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5443988A (en) * | 1992-09-02 | 1995-08-22 | Fuji Photo Film Co., Ltd. | Method for high speed analysis of a dry analytical element |
| CN1639557A (en) * | 2001-04-11 | 2005-07-13 | 伯斯坦技术公司 | Multi-parameter assays including analysis discs and methods relating thereto |
| CN1985174A (en) * | 2004-07-12 | 2007-06-20 | 爱科来株式会社 | Analyzer, method for specifying reaction vessel in analyzer, and analytical apparatus |
| CN101598727A (en) * | 2009-07-09 | 2009-12-09 | 上海科华生物工程股份有限公司 | The drying chemical reagent paper of quantitatively determining human blood urea content |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111426823A (en) * | 2020-04-30 | 2020-07-17 | 益善生物技术股份有限公司 | Reaction cassette for detection and detection operation method |
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Application publication date: 20111123 |