CN102250795B - Gordoniaalkanivorans, microbial agent and use thereof - Google Patents
Gordoniaalkanivorans, microbial agent and use thereof Download PDFInfo
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- CN102250795B CN102250795B CN 201110167185 CN201110167185A CN102250795B CN 102250795 B CN102250795 B CN 102250795B CN 201110167185 CN201110167185 CN 201110167185 CN 201110167185 A CN201110167185 A CN 201110167185A CN 102250795 B CN102250795 B CN 102250795B
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Abstract
The invention provides Gordoniaalkanivorans with a collection number of CGMCCNo.4796, a microbial agent containing the Gordoniaalkanivorans, and the use of the Gordoniaalkanivorans or the microbial agent in degradation of petroleum hydrocarbon, biological remediation of petroleum pollution and biological remediation of soil polluted by petroleum and biological remediation of water polluted by petroleum. The Gordoniaalkanivorans with the collection number of CGMCCNo.4796 and the microbial agent containing the Gordoniaalkanivorans have very high petroleum hydrocarbon degrading capacity and can be widely used for degrading petroleum hydrocarbons and remediating petroleum pollution, such as remediating soil and water, which are polluted by petroleum.
Description
Technical field
The present invention relates to a kind of food alkane Gordon Salmonella (
Gordonia alkanivorans), and a kind of contain this food alkane Gordon Salmonella (
Gordonia alkanivorans) as the microbiobacterial agent of activeconstituents and their application; Or rather, relate to food alkane Gordon Salmonella that a kind of preserving number is CGMCC No. 4796 (
Gordonia alkanivorans), and the microbiobacterial agent that contains this food alkane Gordon Salmonella, also relate to application, the application in the biological restoration petroleum pollution, the application in the soil of biological restoration petroleum pollution and the application in the water body of biological restoration petroleum pollution in decomposing petroleum hydrocarbon of described food alkane Gordon Salmonella or microbiobacterial agent.
Background technology
The world today, oil has become one of topmost energy of the mankind, and a large amount of oil and processed goods thereof enter the petroleum pollution that soil causes soil, bring harm for human mater to whole biosphere, thereby become international environmental problem.In the research to petroleum pollution, biological restoration as a kind of potential efficiently, cleaning technique come into one's own just day by day cheaply.
Biological restoration (Bioremediation) refers to by biological (particularly microorganism) catalyze and degrade organic pollutants, thus the controlled or spontaneous process of carrying out of repairing the pollutent in contaminated environment or elimination environment.
" petroleum hydrocarbon " is the main pollutant component in oil and processed goods thereof, and research finds that microorganism can play Degradation to petroleum hydrocarbon.The microbiological deterioration petroleum hydrocarbon is to find 19 end of the centurys.Before the 1950's, take U.S. C.E. assistant Bel as representative, the marine microorganism decomposing petroleum hydrocarbon is conducted extensive research.The beginning of the fifties, gas-chromatography was come out, and the generally application of radio-label method has play a part positive to the microbiological deterioration mechanism of studying petroleum hydrocarbon.Since the sixties, because offshore oil pollution is on the rise, impel many maritime nations, as states such as the U.S., Canada, Japan, Britain and the Soviet Union, actively developed the research work of relevant marine microorganism decomposing petroleum hydrocarbon.The mid-1970s, American scholar have also been cultivated " super microbe " with engineered technology, to decomposing petroleum hydrocarbon effectively.
China is from 1975, successively on quantity, distribution, the species composition of Jiaozhou Bay of Qingdao, the Bohai Sea, PORT OF XIAMEN, the Huanghai Sea and East Sea oil degradation microorganism with affect degradation factors etc. and investigate.
In affecting the principal element of microorganism to the degraded of petroleum hydrocarbon, the physiological property of microorganism is one of key factor that determines the decomposing petroleum hydrocarbon ability.At present separated the microorganism strains that obtains also lower to the petroleum hydrocarbon degradation ability, therefore, in the urgent need to developing a kind of microorganism strains and relevant microbiobacterial agent that can the efficient degradation petroleum hydrocarbon.
Summary of the invention
The object of the invention is to overcome the existing microorganism strains shortcoming low to the degradation capability of petroleum hydrocarbon, a kind of microorganism strains and relevant microbiobacterial agent and application of decomposing petroleum hydrocarbon efficiently are provided.
In order to realize first goal of the invention, provide a kind of food alkane Gordon Salmonella (
Gordonia alkanivorans), wherein, this food alkane Gordon Salmonella (
Gordonia alkanivorans) deposit number be CGMCC No. 4796.
In order to realize second goal of the invention, a kind of microbiobacterial agent of decomposing petroleum hydrocarbon is provided, wherein, this microbiobacterial agent contain food alkane Gordon Salmonella (
Gordonia alkanivorans) as activeconstituents, wherein, this food alkane Gordon Salmonella (
Gordonia alkanivorans) deposit number be CGMCC No. 4796.
In addition, the present invention also provides described food alkane Gordon Salmonella or the application of described microbiobacterial agent in decomposing petroleum hydrocarbon.
The present invention also provides described food alkane Gordon Salmonella or the application of described microbiobacterial agent in the biological restoration petroleum pollution.
The present invention also provides described food alkane Gordon Salmonella or the application of described microbiobacterial agent in the soil of biological restoration petroleum pollution.
The present invention also provides described food alkane Gordon Salmonella or the application of described microbiobacterial agent in the water body of biological restoration petroleum pollution.
Food alkane Gordon Salmonella of the present invention (
Gordonia alkanivorans) bacterial strain screens by a large amount of screening operations, this bacterial strain has very strong degradation capability to petroleum hydrocarbon.This bacterial strain has been stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and (address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), preserving number was CGMCC No. 4796 on 04 28th, 2011.Preserving number provided by the invention be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and the microbiobacterial agent that contains this bacterium petroleum hydrocarbon is had very strong degradation capability, can be widely used in decomposing petroleum hydrocarbon and to the reparation of petroleum pollution, as to the reparation of the soil of petroleum pollution with to reparation of the water body of petroleum pollution etc.
Description of drawings
Fig. 1 shown the preserving number of the present invention's screening be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) the cultivation form of 24 hours on solid TSA substratum;
Fig. 2 shown the preserving number of the present invention's screening be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) cultivating 24 hours on solid TSA substratum by the form under the rear 100 times of oily mirrors at opticmicroscope of lawn dyeing;
Fig. 3 be preserving number that the present invention screens be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) after cultivating 24 hours on solid TSA substratum stereoscan photograph (* 10K);
Fig. 4 be preserving number that the present invention screens be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) after cultivating 48 hours on solid TSA substratum stereoscan photograph (* 10K).
Embodiment
The invention provides a kind of food alkane Gordon Salmonella (
Gordonia alkanivorans), wherein, this food alkane Gordon Salmonella (
Gordonia alkanivorans) deposit number be CGMCC No. 4796.Through 16s rDNA amplification and sequencing analysis, deposit number is that the bacterial strain of CGMCC No. 4796 has the 16s rDNA sequence shown in SEQ ID NO:1.
The present invention also provides a kind of microbiobacterial agent of decomposing petroleum hydrocarbon, wherein, this microbiobacterial agent contain food alkane Gordon Salmonella (
Gordonia alkanivorans) as activeconstituents, wherein, this food alkane Gordon Salmonella (
Gordonia alkanivorans) deposit number be CGMCC No. 4796.The food alkane Gordon Salmonella that contains in described microbiobacterial agent (
Gordonia alkanivorans) amount can in very large range change, under preferable case, the contained total viable count of the described microbiobacterial agent of every gram can be 0.1-2.5 * 10
10Individual, 0.5-1 * 10 more preferably
10Individual.
According to the present invention, described microbiobacterial agent also contains substratum, and described substratum is one or more in beef-protein medium, oil screening culture medium and PDA substratum, is preferably beef-protein medium.Above-mentioned substratum can be commercially available or prepare according to the record of " microbiological culture media handbook " (Microbiology Culture Media Manual).For example, described beef-protein medium can contain extractum carnis, peptone, NaCl and water, wherein, extractum carnis with respect to 100 weight parts, the content of peptone can be the 150-250 weight part, the content of NaCl can be the 50-150 weight part, and the content of water can be the 15000-25000 weight part, and the pH value can be 7.2-7.4; Add the agar of 1.2 % by weight to can be made into solid medium in substratum.
The present invention also provide preserving number be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and contain the application of microbiobacterial agent in decomposing petroleum hydrocarbon of this bacterial strain.
The present invention also provide preserving number be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and contain the application of microbiobacterial agent in the biological restoration petroleum pollution of this bacterial strain.
The present invention also provide preserving number be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and contain the application of microbiobacterial agent in the soil of biological restoration petroleum pollution of this bacterial strain.
The present invention also provide preserving number be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and contain the application of microbiobacterial agent in the water body of biological restoration petroleum pollution of this bacterial strain.
Below by specific embodiment, the present invention is carried out further description.
Experiment material
One, the oil sample of strain screening:
Pick up from the crude oil of Shengli Oil Field and commercially available 0# diesel oil and mix in the ratio of 1:4, as the experiment oil.
Two, pedotheque is the mixture of 1:1:1 that picks up from three kinds of different oil-polluted soils samples of Shengli Oil Field, Shandong, and it is respectively:
1) the ground crude oil contaminated soil around producing well;
2) oil sediment; With
3) stack for a long time the topsoil soil of oil sediment.
Three,Substratum
1) enrichment medium: K
2HPO
43H
2O 1.0g, KH
2PO
41.0g, MgSO
47H
2O 0.5g, NH
4NO
31.0g, CaCl
20.02g distilled water 1000mL transfers to PH7.0,121 ℃ of sterilization 20min add the 5g oil after cooling.
2) isolation medium (PDA substratum): potato powder 6.0g, glucose 20g, agar 20g adds distilled water until 1000ml, and standby 115 ℃ of autoclavings 20 minutes.
3) strain store medium: beef-protein medium
4) oil screening culture medium (liquid): NaNO
31.5g, (NH4)
2SO
41.5g, K
2HPO
41g, MgSO
47H
2O 0.5 g, KCl 0.5g, FeSO
47H
2O 0.01g, CaCl
20.002g distilled water 1000mL transfers to pH8.0,121 ℃ of sterilization 20min add the 2.5g oil after cooling.Add the agar of 1.2 % by weight to make the solid screening culture medium in substratum.
5) solid TSA substratum: Tryptones 15g, soya peptone 5g, sodium-chlor 30g, agar 15g adds distilled water until final volume is 1000ml, and the pH value is 7.1-7.5.
Embodiment 1
1, the concentration and separation of petroleum hydrocarbon degradation bacterial strain
Taking 5g oil-polluted soils mixture adds in the 250mL triangular flask that the 100mL enrichment medium is housed, cultivate under 28 ℃, the constant-temperature table condition of 180r/min, draw afterwards the 5mL nutrient solution in 5 days and be forwarded to fresh enrichment medium, the same terms was cultivated 5 days, enrichment culture is 25 days so continuously, nutrient solution is through being coated on after gradient dilution on solid PDA substratum, in 28 ℃ of cultivations; After flat board grew bacterium colony, the single bacterium colony of picking different shape adopted the line partition method repeatedly to carry out separation and purification, until obtain the pure growth of single strain, the bacterial strain after purifying is stored in the beef-protein medium inclined-plane.
2, the screening of high degrading activity bacterial strain
The bacterial strain of above-mentioned purifying is inoculated into respectively in the 250mL triangular flask that the 100mL screening culture medium is housed, cultivated 5 days under 28 ℃, the condition of 180r/min, after being diluted 100 times, nutrient solution is coated on the solid screening culture medium, observe the growing state of each bacterial strain in solid medium, filter out grow very fast, the more bacterial strain of bacterium colony.
Single bacterium colony of each bacterial strain of picking is inoculated into respectively in the 250ml triangular flask that 50ml liquid beef-protein medium is housed, cultivated 24 hours in 28 ℃, the constant-temperature table of 180r/min, measure bacterium liquid in the absorbancy at 600nm place, regulate its mass concentration with the beef-protein medium of sterilization and make OD600nm be about 0.4, make seed inoculation screening culture medium with this bacterium liquid that dilutes.
Drawing the ready seed liquor of 5ml is inoculated in the 250mL triangular flask that the 100mL screening culture medium is housed, measure oil content cultivate 4 days in 28 ℃, the constant-temperature table of 180r/min after, the processing triplicate of each seed liquor, calculate average degradation rate, filter out the highest bacterial strain of petroleum degradation rate, and this bacterial strain has been carried out biological preservation, preserving number is CGMCC No. 4796.
Embodiment 2
According to " experiment content and experimental technique of record in common bacteria system identification handbook [4] and " actinomycetes systematic theory, method and put into practice " [5], to sieve preserving number be that the bacterial strain of CGMCC No. 4796 carries out strain identification.
1, morphological analysis
The bacterial strain that is CGMCC No. 4796 to preserving number carries out morphological analysis and observes under opticmicroscope and scanning electron microscope, and result is respectively as shown in Fig. 1-4.
Colonial morphology is observed and is carried out on solid TSA substratum, after the bacterial strain streak inoculation, observes colonial morphology in 28 ℃ of constant temperature culture 24-48 hours.Preserving number is that the bacterium colony of the bacterial strain of CGMCC No. 4796 is orange, circle, smooth texture, rat.Picking is cultivated 24 hours rear 100 times of oily Microscopic observations at opticmicroscope of lawn dyeing.Can clearly see at microscopically, preserving number is the somatic cells less of the bacterial strain of CGMCC No. 4796, and in pairs or gather into Xiao Cong and occur.Find in culturing process preserving number be the cell shape of bacterial strain of CGMCC No. 4796 along with incubation time changes, thus this strain culturing thalli morphology of 24 hours, 48 hours has been done respectively scanning electron microscopic observation, see respectively Fig. 3 and 4.Cultivated 24 hours, thalline all is rod, the cell less; Be cultured to 48 hours, most of thalline are spherical in shape.
2,16s rDNA identifies
The pcr amplification of 16s rDNA and order-checking: the purifying preserving number is total DNA of the bacterial classification of CGMCC No. 4796 to adopt test kit (Beijing full Shi Jin biotech company) to extract also, then take total DNA as template, 16s rDNA conserved sequence is carried out pcr amplification obtain the goal gene fragment.
The pcr amplification primer is respectively:
27f(5 '-AGAGTTTGATCCTGGCTCAG-3 ') and;
1492r（5’-TACGGCTACCTTGTTACGACTT-3’）。
PCR total reaction system is 50uL, specifically comprises:
| 10×Buffer | 5μL |
| 10 mmol/ L dNTP | 4μL |
| Reverse primer | 1μL |
| Forward primer | 1μL |
| Taq enzyme (5U/ μ L) | 1μL |
| Template DNA | 2μL |
| ddH 2O | Complement to 50 μ L |
The PCR reaction conditions is: 94 ℃ of denaturation 5min, and 94 ℃ of sex change 1min, 50 ℃ of annealing 1min, 72 ℃ are extended 2min, and this step is carried out 35 circulations altogether, and last 72 ℃ are extended 10min.
Amplified production carries out the electrophoresis check with 1% sepharose.The examining order of PCR product is completed by Beijing three rich polygala root biotech company.The sequencing result demonstration, preserving number is that the bacterial strain of CGMCC No. 4796 has the 16s rDNA sequence shown in SEQ ID NO:1.
Through Phylogenetic Analysis: log in http://www.ncbi.nlm.nih.gov, check order row are analyzed by the sequence in blast program and nucleic acid database search same or analogous nucleotide sequence, then utilize sequence alignment software ClustalX 2.0 and Phylogenetic Analysis software MEGA 4.0.2 constructing system evolutionary tree.In MEGA software, the method of constructing system evolutionary tree is used the Neighbor-Joining method, the base alternative model is selected the Kimura two-parameter model, the evolutionary tree certificate authenticity is selected 1000 duplicate detection of check (bootstrap) of bootstrapping, and identical weighted value is given in the conversion in the DNA sequence variations and transversion.Show by analysis, preserving number be the bacterial strain of CGMCC No. 4796 belong to food alkane Gordon Salmonella (
Gordonia alkanivorans).
Embodiment 3
Taking 10g oil-polluted soils mixture (oil content 1.25 grams) adds and the 100mL beef-protein medium is housed (preserving number is that the inoculum size of food alkane Gordon Salmonella of CGMCC No. 4796 is that the contained total viable count of every gram substratum is 0.55 * 10
10Individual) the 250mL triangular flask in, cultivate under 28 ℃, the constant-temperature table condition of 180r/min, detected afterwards the oil content in the oil-polluted soils mixture in 3 days, result is 0.49 gram, this bacterial strain is about 60.8% to the degradation rate of oil.
Embodiment 4
Taking 10g oil-polluted soils mixture (oil content 1.25 grams) adds and the 100mL beef-protein medium is housed (preserving number is that the inoculum size of food alkane Gordon Salmonella of CGMCC No. 4796 is that the contained total viable count of every gram substratum is 0.85 * 10
10Individual) the 250mL triangular flask in, cultivate under 28 ℃, the constant-temperature table condition of 180r/min, detected afterwards the oil content in the oil-polluted soils mixture in 3 days, result is 0.42 gram, this bacterial strain is about 66.4% to the degradation rate of oil.
Embodiment 5
Taking 10g oil-polluted soils mixture (oil content 1.25 grams) adds and the 100mL beef-protein medium is housed (preserving number is that the inoculum size of food alkane Gordon Salmonella of CGMCC No. 4796 is that the contained total viable count of every gram substratum is 0.35 * 10
10Individual) the 250mL triangular flask in, cultivate under 28 ℃, the constant-temperature table condition of 180r/min, detected afterwards the oil content in the oil-polluted soils mixture in 3 days, result is 0.51 gram, this bacterial strain is about 59.2% to the degradation rate of oil.
Can be found out by the above results, the preserving number that the present invention filters out is that food alkane Gordon Salmonella of CGMCC No. 4796 has good degradation capability to petroleum hydrocarbon, can be widely used in decomposing petroleum hydrocarbon and to the reparation of petroleum pollution, as to the reparation of the soil of petroleum pollution with to reparation of the water body of petroleum pollution etc.
SEQUENCE LISTING
＜110〉Institute of Environment and Sustainable Development in Agriculture, CAAS
＜120〉a kind ofly eat alkane Gordon Salmonella and microbiobacterial agent and their application
<160> 1
<170> PatentIn version 3.4
<210> 1
<211> 1408
<212> DNA
＜213〉food alkane Gordon Salmonella (
Gordoniaalkanivorans) 16s rDNA
<400> 1
gcttacacat gcaagtcgaa cggaaaggcc cagcttgctg ggtactcgag tggcgaacgg 60
gtgagtaaca cgtgggtgat ctgccctgaa ctttgggata agcctgggaa actgggtcta 120
ataccggata tgaccttgga gtgcatgctc tggggtggaa agcttttgcg gttcaggatg 180
ggcccgcggc ctatcagctt gttggtgggg taatggccta ccaaggcgac gacgggtagc 240
cgacctgaga gggtgatcgg ccacactggg actgagacac ggcccagact cctacgggag 300
gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagcgacgc cgcgtgaggg 360
atgacggcct tcgggttgta aacctctttc accagggacg aagcgcaagt gacggtacct 420
ggagaagaag caccggccaa ctacgtgcca gcagccgcgg taatacgtag ggtgcgagcg 480
ttgtccggaa ttactgggcg taaagagctc gtaggcggtt tgtcgcgtcg tctgtgaaat 540
tctgcaactc aattgtaggc gtgcaggcga tacgggcaga cttgagtact acaggggaga 600
ctggaattcc tggtgtagcg gtgaaatgcg cagatatcag gaggaacacc ggtggcgaag 660
gcgggtctct gggtagtaac tgacgctgag gagcgaaagc gtgggtagcg aacaggatta 720
gataccctgg tagtccacgc cgtaaacggt gggtactagg tgtggggctc atttcacgag 780
ttccgtgccg tagctaacgc attaagtacc ccgcctgggg agtacggccg caaggctaaa 840
actcaaagga attgacgggg gcccgcacaa gcggcggagc atgtggatta attcgatgca 900
acgcgaagaa ccttacctgg gtttgacata caccagacgc atgtagagat acatgttccc 960
ttgtggttgg tgtacaggtg gtgcatggct gtcgtcagct cgtgtcgtga gatgttgggt 1020
taagtcccgc aacgagcgca acccttgtcc tgtattgcca gcgggttatg ccggggactt 1080
gcaggagact gccggggtca actcggagga aggtggggat gacgtcaagt catcatgccc 1140
cttatgtcca gggcttcaca catgctacaa tggctggtac agagggctgc gataccgtga 1200
ggtggagcga atcccttaaa gccagtctca gttcggattg gggtctgcaa ctcgacccca 1260
tgaagtcgga gtcgctagta atcgcagatc agcaacgctg cggtgaatac gttcccgggc 1320
cttgtacaca ccgcccgtca cgtcatgaaa gtcggtaaca cccgaagccg gtggcctaac 1380
cccttgtggg agggagctgt cgaaggtg 1408
Claims (10)
- Food alkane Gordon Salmonella ( Gordonia alkanivorans), it is characterized in that, this food alkane Gordon Salmonella ( Gordonia alkanivorans) deposit number be CGMCC No. 4796.
- 2. the microbiobacterial agent of a decomposing petroleum hydrocarbon, is characterized in that, this microbiobacterial agent contain food alkane Gordon Salmonella ( Gordonia alkanivorans) as activeconstituents, wherein, this food alkane Gordon Salmonella ( Gordonia alkanivorans) deposit number be CGMCC No. 4796.
- 3. microbiobacterial agent according to claim 2, wherein, the contained total viable count of the described microbiobacterial agent of every gram is 0.1-2.5 * 10 10Individual.
- 4. microbiobacterial agent according to claim 3, wherein, the contained total viable count of the described microbiobacterial agent of every gram is 0.5-1 * 10 10Individual.
- 5. the described microbiobacterial agent of any one according to claim 2-4, wherein, this microbiobacterial agent also contains substratum, and described substratum is one or more in beef-protein medium, oil screening culture medium and PDA substratum.
- 6. microbiobacterial agent according to claim 5, wherein, described substratum is beef-protein medium.
- 7. the application of the described microbiobacterial agent of any one in decomposing petroleum hydrocarbon in food alkane Gordon Salmonella claimed in claim 1 or claim 2-6.
- 8. the application of the described microbiobacterial agent of any one in the biological restoration petroleum pollution in food alkane Gordon Salmonella claimed in claim 1 or claim 2-6.
- 9. the application of the described microbiobacterial agent of any one in the soil of biological restoration petroleum pollution in food alkane Gordon Salmonella claimed in claim 1 or claim 2-6.
- 10. the application of the described microbiobacterial agent of any one in the water body of biological restoration petroleum pollution in food alkane Gordon Salmonella claimed in claim 1 or claim 2-6.
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| CN102703348B (en) * | 2012-05-25 | 2013-08-21 | 武汉科技大学 | Alkane degrading bacteria and application thereof |
| CN103232960B (en) * | 2013-04-23 | 2014-12-03 | 中国海洋石油总公司 | Oil-degrading composite bacterium and microbial inoculum applied to high salt environment |
| CN112608862B (en) * | 2020-12-21 | 2022-04-15 | 中国科学院南海海洋研究所 | Petroleum hydrocarbon degradation functional bacterium SCSIO19801 and application thereof |
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| JP2008289445A (en) * | 2007-05-28 | 2008-12-04 | Tosoh Corp | Petroleum-decomposing microorganism, microorganism consortium, and method for cleaning petroleum-contaminated soil by using the same |
| CN101974466A (en) * | 2010-10-28 | 2011-02-16 | 大连理工大学 | Gordonia alkanivorans and application in oil degradation |
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2011
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008289445A (en) * | 2007-05-28 | 2008-12-04 | Tosoh Corp | Petroleum-decomposing microorganism, microorganism consortium, and method for cleaning petroleum-contaminated soil by using the same |
| CN101974466A (en) * | 2010-10-28 | 2011-02-16 | 大连理工大学 | Gordonia alkanivorans and application in oil degradation |
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| Title |
|---|
| "Isolation and Characterization of a Diesel-Degrading Bacterium, Gordonia sp SD8";Hong, Sunhwa;《Korean Journal of Microbiology and Biotechnology》;20100930;第38卷(第3期);335-339 * |
| "石油烃降解菌的筛选与鉴定";崔丽虹;《生物技术通报》;20090930(第9期);143-147 * |
| Hong, Sunhwa."Isolation and Characterization of a Diesel-Degrading Bacterium, Gordonia sp SD8".《Korean Journal of Microbiology and Biotechnology》.2010,第38卷(第3期), |
| 崔丽虹."石油烃降解菌的筛选、鉴定及复合菌群降解效果的研究".《中国优秀硕士学位论文全文数据库（工程科技Ⅰ辑）》.2009,(第10期), |
| 崔丽虹."石油烃降解菌的筛选与鉴定".《生物技术通报》.2009,(第9期), |
| 崔丽虹."石油烃降解菌的筛选、鉴定及复合菌群降解效果的研究".《中国优秀硕士学位论文全文数据库(工程科技Ⅰ辑)》.2009,(第10期), * |
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