CN102250795A - Gordoniaalkanivorans, microbial agent and use thereof - Google Patents
Gordoniaalkanivorans, microbial agent and use thereof Download PDFInfo
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- CN102250795A CN102250795A CN2011101671851A CN201110167185A CN102250795A CN 102250795 A CN102250795 A CN 102250795A CN 2011101671851 A CN2011101671851 A CN 2011101671851A CN 201110167185 A CN201110167185 A CN 201110167185A CN 102250795 A CN102250795 A CN 102250795A
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Abstract
The invention provides Gordoniaalkanivorans with a collection number of CGMCCNo.4796, a microbial agent containing the Gordoniaalkanivorans, and the use of the Gordoniaalkanivorans or the microbial agent in degradation of petroleum hydrocarbon, biological remediation of petroleum pollution and biological remediation of soil polluted by petroleum and biological remediation of water polluted by petroleum. The Gordoniaalkanivorans with the collection number of CGMCCNo.4796 and the microbial agent containing the Gordoniaalkanivorans have very high petroleum hydrocarbon degrading capacity and can be widely used for degrading petroleum hydrocarbons and remediating petroleum pollution, such as remediating soil and water, which are polluted by petroleum.
Description
Technical field
The present invention relates to a kind of food alkane Gordon Salmonella (
Gordonia alkanivorans), and a kind of contain this food alkane Gordon Salmonella (
Gordonia alkanivorans) as the microbiobacterial agent of activeconstituents and their application; Or rather, relate to food alkane Gordon Salmonella that a kind of preserving number is CGMCC No. 4796 (
Gordonia alkanivorans), and the microbiobacterial agent that contains this food alkane Gordon Salmonella, also relate to application, the application in the biological restoration petroleum pollution, the application in the soil of biological restoration petroleum pollution and the application in the water body of biological restoration petroleum pollution in decomposing petroleum hydrocarbon of described food alkane Gordon Salmonella or microbiobacterial agent.
Background technology
The world today, oil has become one of human topmost energy, and a large amount of oil and processed goods thereof enter the petroleum pollution that soil causes soil, bring harm for human mater to whole biosphere, thereby become international environmental problem.In the research to petroleum pollution, biological restoration as a kind of potential efficiently, cleaning technique come into one's own just day by day cheaply.
Biological restoration (Bioremediation) is meant by biological (particularly microorganism) catalyze and degrade organic pollutants, thus the controlled or spontaneous process of carrying out of repairing the pollutent in contaminated environment or the elimination environment.
" petroleum hydrocarbon " is the main pollutant component in oil and the processed goods thereof, discovers that microorganism can play Degradation to petroleum hydrocarbon.The microbiological deterioration petroleum hydrocarbon is to find for 19 end of the centurys.Before the 1950's, Bel is representative with U.S. C.E. assistant, and the marine microorganism decomposing petroleum hydrocarbon has been carried out extensive studies.The beginning of the fifties, gas-chromatography was come out, and the widespread usage of radio-label method has play a part positive to the microbiological deterioration mechanism of studying petroleum hydrocarbon.Since the sixties,, impelled many maritime nations,, actively developed the research work of relevant marine microorganism decomposing petroleum hydrocarbon as states such as the U.S., Canada, Japan, Britain and the Soviet Union because offshore oil pollution is on the rise.The mid-1970s, American scholar have also been cultivated " super microorganism " with engineered technology, in the hope of decomposing petroleum hydrocarbon effectively.
China is from 1975, successively Jiaozhou Bay, Qingdao, the Bohai Sea, PORT OF XIAMEN, the Huanghai Sea and East Sea oil degradation microbial numbers, distribution, kind formed and influenced degradation factors etc. and investigate.
In influencing the principal element of microorganism to the degraded of petroleum hydrocarbon, the physiological property of microorganism is one of key factor of decision decomposing petroleum hydrocarbon ability.It is also lower to the petroleum hydrocarbon degradation ability at present to have separated the microorganism strains that obtains, and therefore, presses for and develops a kind of microorganism strains and relevant microbiobacterial agent that can the efficient degradation petroleum hydrocarbon.
Summary of the invention
The objective of the invention is to overcome the existing microorganism strains shortcoming low, a kind of microorganism strains and relevant microbiobacterial agent and application of decomposing petroleum hydrocarbon efficiently are provided the degradation capability of petroleum hydrocarbon.
In order to realize first goal of the invention, provide a kind of food alkane Gordon Salmonella (
Gordonia alkanivorans), wherein, this food alkane Gordon Salmonella (
Gordonia alkanivorans) deposit number be CGMCC No. 4796.
In order to realize second goal of the invention, a kind of microbiobacterial agent of decomposing petroleum hydrocarbon is provided, wherein, this microbiobacterial agent contain food alkane Gordon Salmonella (
Gordonia alkanivorans) as activeconstituents, wherein, this food alkane Gordon Salmonella (
Gordonia alkanivorans) deposit number be CGMCC No. 4796.
In addition, the present invention also provides described food alkane Gordon Salmonella or the application of described microbiobacterial agent in decomposing petroleum hydrocarbon.
The present invention also provides described food alkane Gordon Salmonella or the application of described microbiobacterial agent in the biological restoration petroleum pollution.
The present invention also provides described food alkane Gordon Salmonella or the application of described microbiobacterial agent in the soil of biological restoration petroleum pollution.
The present invention also provides described food alkane Gordon Salmonella or the application of described microbiobacterial agent in the water body of biological restoration petroleum pollution.
Food alkane Gordon Salmonella of the present invention (
Gordonia alkanivorans) bacterial strain screens by a large amount of screening operations, this bacterial strain has very strong degradation capability to petroleum hydrocarbon.This bacterial strain has been stored in China Committee for Culture Collection of Microorganisms common micro-organisms center, and (address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), preserving number was CGMCC No. 4796 on 04 28th, 2011.Preserving number provided by the invention be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and the microbiobacterial agent that contains this bacterium petroleum hydrocarbon is had very strong degradation capability, can be widely used in decomposing petroleum hydrocarbon and to the reparation of petroleum pollution, as to the reparation of the soil of petroleum pollution with to reparation of the water body of petroleum pollution etc.
Description of drawings
Fig. 1 shown the preserving number of the present invention's screening be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) 24 hours form of cultivation on solid TSA substratum;
Fig. 2 shown the preserving number of the present invention's screening be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) cultivating 24 hours on the solid TSA substratum after the form under 100 times of oily mirrors of lawn dyeing back at opticmicroscope;
Fig. 3 for the preserving number of the present invention screening be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) after cultivating 24 hours on the solid TSA substratum stereoscan photograph (* 10K);
Fig. 4 for the preserving number of the present invention screening be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) after cultivating 48 hours on the solid TSA substratum stereoscan photograph (* 10K).
Embodiment
The invention provides a kind of food alkane Gordon Salmonella (
Gordonia alkanivorans), wherein, this food alkane Gordon Salmonella (
Gordonia alkanivorans) deposit number be CGMCC No. 4796.Through 16s rDNA amplification and sequencing analysis, deposit number is that the bacterial strain of CGMCC No. 4796 has the 16s rDNA sequence shown in the SEQ ID NO:1.
The present invention also provides a kind of microbiobacterial agent of decomposing petroleum hydrocarbon, wherein, this microbiobacterial agent contain food alkane Gordon Salmonella (
Gordonia alkanivorans) as activeconstituents, wherein, this food alkane Gordon Salmonella (
Gordonia alkanivorans) deposit number be CGMCC No. 4796.The food alkane Gordon Salmonella that contains in the described microbiobacterial agent (
Gordonia alkanivorans) amount can in very large range change, under the preferable case, the contained total viable count of the described microbiobacterial agent of every gram can be 0.1-2.5 * 10
10Individual, 0.5-1 * 10 more preferably
10Individual.
According to the present invention, described microbiobacterial agent also contains substratum, and described substratum is one or more in beef-protein medium, oil screening culture medium and the PDA substratum, is preferably beef-protein medium.Above-mentioned substratum can be commercially available or prepare according to the record of " microbiological culture media handbook " (Microbiology Culture Media Manual).For example, described beef-protein medium can contain extractum carnis, peptone, NaCl and water, wherein, extractum carnis with respect to 100 weight parts, the content of peptone can be the 150-250 weight part, the content of NaCl can be the 50-150 weight part, and the content of water can be the 15000-25000 weight part, and the pH value can be 7.2-7.4; The agar that adds 1.2 weight % in the substratum can be made into solid medium.
The present invention also provide preserving number be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and contain the application of microbiobacterial agent in decomposing petroleum hydrocarbon of this bacterial strain.
The present invention also provide preserving number be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and contain the application of microbiobacterial agent in the biological restoration petroleum pollution of this bacterial strain.
The present invention also provide preserving number be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and contain the application of microbiobacterial agent in the soil of biological restoration petroleum pollution of this bacterial strain.
The present invention also provide preserving number be CGMCC No. 4796 food alkane Gordon Salmonella (
Gordonia alkanivorans) and contain the application of microbiobacterial agent in the water body of biological restoration petroleum pollution of this bacterial strain.
Below by specific embodiment the present invention is further illustrated.
Experiment material
One, the oil sample of strain screening:
The crude oil and the commercially available 0# diesel oil that pick up from Shengli Oil Field are even by the mixed of 1:4, as the experiment oil.
Two, pedotheque is the mixture of 1:1:1 that picks up from three kinds of different oil-polluted soils samples of Shandong Shengli Oil Field, and it is respectively:
1) the ground crude oil contaminated soil around the producing well;
2) oil sediment; With
3) stack the topsoil soil of oil sediment for a long time.
Three,Substratum
1) enrichment medium: K
2HPO
43H
2O 1.0g, KH
2PO
41.0g, MgSO
47H
2O 0.5g, NH
4NO
31.0g, CaCl
20.02g distilled water 1000mL transfers to PH7.0,121 ℃ of sterilization 20min add the 5g oil after cooling.
2) isolation medium (PDA substratum): potato powder 6.0g, glucose 20g, agar 20g adds distilled water until 1000ml, and standby 115 ℃ of autoclavings 20 minutes.
3) strain store medium: beef-protein medium
4) oil screening culture medium (liquid): NaNO
31.5g, (NH4)
2SO
41.5g, K
2HPO
41g, MgSO
47H
2O 0.5 g, KCl 0.5g, FeSO
47H
2O 0.01g, CaCl
20.002g distilled water 1000mL transfers to pH8.0,121 ℃ of sterilization 20min, and the cooling back adds the 2.5g oil.The agar that adds 1.2 weight % in the substratum is made the solid screening culture medium.
5) solid TSA substratum: Tryptones 15g, soya peptone 5g, sodium-chlor 30g, agar 15g, adding distilled water is 1000ml until final volume, the pH value is 7.1-7.5.
Embodiment 1
1, the concentration and separation of petroleum hydrocarbon degradation bacterial strain
Taking by weighing the adding of 5g oil-polluted soils mixture is equipped with in the 250mL triangular flask of 100mL enrichment medium, under 28 ℃, the constant temperature shaking table condition of 180r/min, cultivate, draw the 5mL nutrient solution after 5 days and be forwarded to fresh enrichment medium, the same terms was cultivated 5 days, enrichment culture is 25 days so continuously, nutrient solution is through being coated on behind the gradient dilution on the solid PDA substratum, in 28 ℃ of cultivations; After treating that flat board grows bacterium colony, single bacterium colony of picking different shape adopts the line partition method to carry out separation and purification repeatedly, and up to the pure growth that obtains single strain, the bacterial strain behind the purifying is stored in the beef-protein medium inclined-plane.
2, the screening of high degrading activity bacterial strain
The bacterial strain of above-mentioned purifying is inoculated into respectively in the 250mL triangular flask that the 100mL screening culture medium is housed, cultivated 5 days under 28 ℃, the condition of 180r/min, be coated on the solid screening culture medium after 100 times of the nutrient solution dilutions, observe the growing state of each bacterial strain in solid medium, filter out grow very fast, the more bacterial strain of bacterium colony.
Single bacterium colony of each bacterial strain of picking is inoculated into respectively in the 250ml triangular flask that 50ml liquid beef-protein medium is housed, in 28 ℃, the constant temperature shaking table of 180r/min, cultivated 24 hours, measure the absorbancy of bacterium liquid at the 600nm place, regulate its mass concentration with the beef-protein medium of sterilization and make OD600nm be about 0.4, make seed inoculation screening culture medium with this bacterium liquid that dilutes.
Drawing the ready seed liquor of 5ml is inoculated in the 250mL triangular flask that the 100mL screening culture medium is housed, measure oil content after in 28 ℃, the constant temperature shaking table of 180r/min, cultivating 4 days, the processing triplicate of each seed liquor, calculate average degradation rate, filter out the highest bacterial strain of oil degradation rate, and this bacterial strain carried out biological preservation, preserving number is CGMCC No. 4796.
Embodiment 2
According to experiment content and experimental technique of record in " common bacteria system identification handbook " [4] and " actinomycetes systematics principle, method and put into practice " [5], to sieve preserving number be that the bacterial strain of CGMCC No. 4796 carries out strain identification.
1, morphological analysis
To preserving number is that the bacterial strain of CGMCC No. 4796 carries out morphological analysis and observes under opticmicroscope and scanning electron microscope, and the result is respectively shown in Fig. 1-4.
Colonial morphology is observed and is carried out on solid TSA substratum, after the bacterial strain streak inoculation, observes colonial morphology in 28 ℃ of constant temperature culture 24-48 hours.Preserving number is that the bacterium colony of the bacterial strain of CGMCC No. 4796 is orange, circle, and quality is smooth, rat.Picking is cultivated 24 hours lawn dyeing backs and is observed under 100 times of oily mirrors of opticmicroscope.Can see clearly that at microscopically preserving number is that the somatic cells of bacterial strain of CGMCC No. 4796 is less relatively, and in pairs or gather into Xiao Cong and occur.In culturing process, find preserving number be the cell shape of bacterial strain of CGMCC No. 4796 along with incubation time changes, thus 24 hours, 48 hours thalli morphology of this strain culturing has been done scanning electron microscopic observation respectively, see Fig. 3 and 4 respectively.Cultivated 24 hours, thalline all is rod, and cell is less relatively; Be cultured to 48 hours, most of thalline are spherical in shape.
2,16s rDNA identifies
The pcr amplification of 16s rDNA and order-checking: the purifying preserving number is total DNA of the bacterial classification of CGMCC No. 4796 to adopt test kit (the full Shi Jin in Beijing biotech company) to extract also, be template with total DNA then, 16s rDNA conserved sequence carried out pcr amplification obtain target gene fragment.
The pcr amplification primer is respectively:
27f(5 '-AGAGTTTGATCCTGGCTCAG-3 ') and;
1492r（5’-TACGGCTACCTTGTTACGACTT-3’）。
PCR total reaction system is 50uL, specifically comprises:
| 10×Buffer | 5μL |
| 10 mmol/ L dNTP | 4μL |
| Reverse primer | 1μL |
| Forward primer | 1μL |
| Taq enzyme (5U/ μ L) | 1μL |
| Template DNA | 2μL |
| ddH 2O | Complement to 50 μ L |
The PCR reaction conditions is: 94 ℃ of pre-sex change 5min, and 94 ℃ of sex change 1min, 50 ℃ of annealing 1min, 72 ℃ are extended 2min, and this step is carried out 35 circulations altogether, and last 72 ℃ are extended 10min.
Amplified production carries out the electrophoresis check with 1% sepharose.The examining order of PCR product is finished by Beijing three rich polygala root biotech companies.Sequencing result shows that preserving number is that the bacterial strain of CGMCC No. 4796 has the 16s rDNA sequence shown in the SEQ ID NO:1.
Through Phylogenetic Analysis: land http://www.ncbi.nlm.nih.gov, check order row are analyzed by the sequence in blast program and the nucleic acid database search same or analogous nucleotide sequence, utilize sequence alignment software ClustalX 2.0 and Phylogenetic Analysis software MEGA 4.0.2 constructing system evolutionary tree then.In MEGA software, the method of constructing system evolutionary tree is used the Neighbor-Joining method, the base alternative model is selected the Kimura two-parameter model, the evolutionary tree certificate authenticity is selected 1000 duplicate detection of check (bootstrap) of bootstrapping for use, and the conversion in the DNA sequence variations is given identical weighted value with transversion.Show by analysis, preserving number be the bacterial strain of CGMCC No. 4796 belong to food alkane Gordon Salmonella (
Gordonia alkanivorans).
Embodiment 3
Taking by weighing 10g oil-polluted soils mixture (oil content 1.25 gram) adds and the 100mL beef-protein medium is housed (preserving number is that the inoculum size of food alkane Gordon Salmonella of CGMCC No. 4796 is that the contained total viable count of every gram substratum is 0.55 * 10
10Individual) the 250mL triangular flask in, under 28 ℃, the constant temperature shaking table condition of 180r/min, cultivate, detect the oil content in the oil-polluted soils mixture after 3 days, the result is 0.49 gram, this bacterial strain is about 60.8% to the degradation rate of oil.
Embodiment 4
Taking by weighing 10g oil-polluted soils mixture (oil content 1.25 gram) adds and the 100mL beef-protein medium is housed (preserving number is that the inoculum size of food alkane Gordon Salmonella of CGMCC No. 4796 is that the contained total viable count of every gram substratum is 0.85 * 10
10Individual) the 250mL triangular flask in, under 28 ℃, the constant temperature shaking table condition of 180r/min, cultivate, detect the oil content in the oil-polluted soils mixture after 3 days, the result is 0.42 gram, this bacterial strain is about 66.4% to the degradation rate of oil.
Embodiment 5
Taking by weighing 10g oil-polluted soils mixture (oil content 1.25 gram) adds and the 100mL beef-protein medium is housed (preserving number is that the inoculum size of food alkane Gordon Salmonella of CGMCC No. 4796 is that the contained total viable count of every gram substratum is 0.35 * 10
10Individual) the 250mL triangular flask in, under 28 ℃, the constant temperature shaking table condition of 180r/min, cultivate, detect the oil content in the oil-polluted soils mixture after 3 days, the result is 0.51 gram, this bacterial strain is about 59.2% to the degradation rate of oil.
By The above results as can be seen, the preserving number that the present invention filters out is that food alkane Gordon Salmonella of CGMCC No. 4796 has good degradation capability to petroleum hydrocarbon, can be widely used in decomposing petroleum hydrocarbon and to the reparation of petroleum pollution, as to the reparation of the soil of petroleum pollution with to reparation of the water body of petroleum pollution etc.
SEQUENCE LISTING
＜110〉Institute of Environment and Sustainable Development in Agriculture, CAAS
＜120〉a kind ofly eat alkane Gordon Salmonella and microbiobacterial agent and their application
<160> 1
<170> PatentIn version 3.4
<210> 1
<211> 1408
<212> DNA
＜213〉food alkane Gordon Salmonella (
Gordoniaalkanivorans) 16s rDNA
<400> 1
gcttacacat gcaagtcgaa cggaaaggcc cagcttgctg ggtactcgag tggcgaacgg 60
gtgagtaaca cgtgggtgat ctgccctgaa ctttgggata agcctgggaa actgggtcta 120
ataccggata tgaccttgga gtgcatgctc tggggtggaa agcttttgcg gttcaggatg 180
ggcccgcggc ctatcagctt gttggtgggg taatggccta ccaaggcgac gacgggtagc 240
cgacctgaga gggtgatcgg ccacactggg actgagacac ggcccagact cctacgggag 300
gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagcgacgc cgcgtgaggg 360
atgacggcct tcgggttgta aacctctttc accagggacg aagcgcaagt gacggtacct 420
ggagaagaag caccggccaa ctacgtgcca gcagccgcgg taatacgtag ggtgcgagcg 480
ttgtccggaa ttactgggcg taaagagctc gtaggcggtt tgtcgcgtcg tctgtgaaat 540
tctgcaactc aattgtaggc gtgcaggcga tacgggcaga cttgagtact acaggggaga 600
ctggaattcc tggtgtagcg gtgaaatgcg cagatatcag gaggaacacc ggtggcgaag 660
gcgggtctct gggtagtaac tgacgctgag gagcgaaagc gtgggtagcg aacaggatta 720
gataccctgg tagtccacgc cgtaaacggt gggtactagg tgtggggctc atttcacgag 780
ttccgtgccg tagctaacgc attaagtacc ccgcctgggg agtacggccg caaggctaaa 840
actcaaagga attgacgggg gcccgcacaa gcggcggagc atgtggatta attcgatgca 900
acgcgaagaa ccttacctgg gtttgacata caccagacgc atgtagagat acatgttccc 960
ttgtggttgg tgtacaggtg gtgcatggct gtcgtcagct cgtgtcgtga gatgttgggt 1020
taagtcccgc aacgagcgca acccttgtcc tgtattgcca gcgggttatg ccggggactt 1080
gcaggagact gccggggtca actcggagga aggtggggat gacgtcaagt catcatgccc 1140
cttatgtcca gggcttcaca catgctacaa tggctggtac agagggctgc gataccgtga 1200
ggtggagcga atcccttaaa gccagtctca gttcggattg gggtctgcaa ctcgacccca 1260
tgaagtcgga gtcgctagta atcgcagatc agcaacgctg cggtgaatac gttcccgggc 1320
cttgtacaca ccgcccgtca cgtcatgaaa gtcggtaaca cccgaagccg gtggcctaac 1380
cccttgtggg agggagctgt cgaaggtg 1408
Claims (10)
- Food alkane Gordon Salmonella ( Gordonia alkanivorans), it is characterized in that, this food alkane Gordon Salmonella ( Gordonia alkanivorans) deposit number be CGMCC No. 4796.
- 2. the microbiobacterial agent of a decomposing petroleum hydrocarbon is characterized in that, this microbiobacterial agent contain food alkane Gordon Salmonella ( Gordonia alkanivorans) as activeconstituents, wherein, this food alkane Gordon Salmonella ( Gordonia alkanivorans) deposit number be CGMCC No. 4796.
- 3. microbiobacterial agent according to claim 2, wherein, the contained total viable count of the described microbiobacterial agent of every gram is 0.1-2.5 * 10 10Individual.
- 4. microbiobacterial agent according to claim 3, wherein, the contained total viable count of the described microbiobacterial agent of every gram is 0.5-1 * 10 10Individual.
- 5. according to any described microbiobacterial agent among the claim 2-4, wherein, this microbiobacterial agent also contains substratum, and described substratum is one or more in beef-protein medium, oil screening culture medium and the PDA substratum.
- 6. microbiobacterial agent according to claim 5, wherein, described substratum is a beef-protein medium.
- 7. any application of described microbiobacterial agent in decomposing petroleum hydrocarbon among the described food alkane of claim 1 Gordon Salmonella or the claim 2-6.
- 8. any application of described microbiobacterial agent in the biological restoration petroleum pollution among the described food alkane of claim 1 Gordon Salmonella or the claim 2-6.
- 9. any application of described microbiobacterial agent in the soil of biological restoration petroleum pollution among the described food alkane of claim 1 Gordon Salmonella or the claim 2-6.
- 10. any application of described microbiobacterial agent in the water body of biological restoration petroleum pollution among the described food alkane of claim 1 Gordon Salmonella or the claim 2-6.
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| CN112608862A (en) * | 2020-12-21 | 2021-04-06 | 中国科学院南海海洋研究所 | Petroleum hydrocarbon degradation functional bacterium SCSIO19801 and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703348A (en) * | 2012-05-25 | 2012-10-03 | 武汉科技大学 | Alkane degrading bacteria and application thereof |
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| CN103232960A (en) * | 2013-04-23 | 2013-08-07 | 中国海洋石油总公司 | Oil-degrading composite bacterium and microbial inoculum applied to high salt environment |
| CN103232960B (en) * | 2013-04-23 | 2014-12-03 | 中国海洋石油总公司 | Oil-degrading composite bacterium and microbial inoculum applied to high salt environment |
| CN112608862A (en) * | 2020-12-21 | 2021-04-06 | 中国科学院南海海洋研究所 | Petroleum hydrocarbon degradation functional bacterium SCSIO19801 and application thereof |
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