CN102250798B - Pseudomonas and microbial agent as well as applications thereof - Google Patents
Pseudomonas and microbial agent as well as applications thereof Download PDFInfo
- Publication number
- CN102250798B CN102250798B CN 201110167206 CN201110167206A CN102250798B CN 102250798 B CN102250798 B CN 102250798B CN 201110167206 CN201110167206 CN 201110167206 CN 201110167206 A CN201110167206 A CN 201110167206A CN 102250798 B CN102250798 B CN 102250798B
- Authority
- CN
- China
- Prior art keywords
- pseudomonas
- rhodopseudomonas
- microbiobacterial agent
- petroleum
- cgmcc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The invention provides pseudomonas with the collection number of CGMCC (China General Microbiological Culture Collection Center) No.4793, a microbial agent containing the pseudomonas, and applications of the pseudomonas or the microbial agent in petroleum hydrocarbon degradation, petroleum pollution bioremediation, petroleum polluted soil bioremediation and petroleum polluted water bioremediation. The pseudomonas with the collection number of CGMCC No.4793 and the microbial agent containing the pseudomonas have strong degrading capability for the petroleum hydrocarbon, can be widely applied to petroleum hydrocarbon degradation and petroleum pollution renovation, petroleum polluted soil renovation, petroleum polluted water renovation and the like.
Description
Technical field
The present invention relates to a kind of Rhodopseudomonas (Pseudomonas sp), and a kind ofly contain this Rhodopseudomonas (Pseudomonas) as the microbiobacterial agent of activeconstituents and their application; Or rather, relate to the Rhodopseudomonas that a kind of preserving number is CGMCC No.4793 (Pseudomonas sp), and the microbiobacterial agent that contains this Rhodopseudomonas, also relate to application, the application in the biological restoration petroleum pollution, the application in the soil of biological restoration petroleum pollution and the application in the water body of biological restoration petroleum pollution in decomposing petroleum hydrocarbon of described Rhodopseudomonas or microbiobacterial agent.
Background technology
The world today, oil has become one of topmost energy of the mankind, and a large amount of oil and processed goods thereof enter the petroleum pollution that soil causes soil, bring harm for human mater to whole biosphere, thereby become international environmental problem.In the research to petroleum pollution, biological restoration as a kind of potential efficiently, cleaning technique come into one's own just day by day cheaply.
Biological restoration (Bioremediation) refers to by biological (particularly microorganism) catalyze and degrade organic pollutants, thus the controlled or spontaneous process of carrying out of repairing the pollutent in contaminated environment or elimination environment.
" petroleum hydrocarbon " is the main pollutant component in oil and processed goods thereof, and research finds that microorganism can play Degradation to petroleum hydrocarbon.The microbiological deterioration petroleum hydrocarbon is to find 19 end of the centurys.Before the 1950's, take U.S. C.E. assistant Bel as representative, the marine microorganism decomposing petroleum hydrocarbon is conducted extensive research.The beginning of the fifties, gas-chromatography was come out, and the generally application of radio-label method has play a part positive to the microbiological deterioration mechanism of studying petroleum hydrocarbon.Since the sixties, because offshore oil pollution is on the rise, impel many maritime nations, as states such as the U.S., Canada, Japan, Britain and the Soviet Union, actively developed the research work of relevant marine microorganism decomposing petroleum hydrocarbon.The mid-1970s, American scholar have also been cultivated " super microbe " with engineered technology, to decomposing petroleum hydrocarbon effectively.
China is from 1975, successively on quantity, distribution, the species composition of Jiaozhou Bay of Qingdao, the Bohai Sea, PORT OF XIAMEN, the Huanghai Sea and East Sea oil degradation microorganism with affect degradation factors etc. and investigate.
In affecting the principal element of microorganism to the degraded of petroleum hydrocarbon, the physiological property of microorganism is one of key factor that determines the decomposing petroleum hydrocarbon ability.At present separated the microorganism strains that obtains also lower to the petroleum hydrocarbon degradation ability, therefore, in the urgent need to developing a kind of microorganism strains and relevant microbiobacterial agent that can the efficient degradation petroleum hydrocarbon.
Summary of the invention
The object of the invention is to overcome the existing microorganism strains shortcoming low to the degradation capability of petroleum hydrocarbon, a kind of microorganism strains and relevant microbiobacterial agent and application of decomposing petroleum hydrocarbon efficiently are provided.
In order to realize first goal of the invention, a kind of Rhodopseudomonas (Pseudomonas sp) is provided, wherein, the deposit number of this Rhodopseudomonas (Pseudomonas sp) is CGMCC No.4793.
In order to realize second goal of the invention, a kind of microbiobacterial agent of decomposing petroleum hydrocarbon is provided, wherein, this microbiobacterial agent contains Rhodopseudomonas (Pseudomonas sp) as activeconstituents, wherein, the deposit number of this Rhodopseudomonas (Pseudomonas sp) is CGMCC No.4793.
In addition, the present invention also provides described Rhodopseudomonas or the application of described microbiobacterial agent in decomposing petroleum hydrocarbon.
The present invention also provides described Rhodopseudomonas or the application of described microbiobacterial agent in the biological restoration petroleum pollution.
The present invention also provides described Rhodopseudomonas or the application of described microbiobacterial agent in the soil of biological restoration petroleum pollution.
The present invention also provides described Rhodopseudomonas or the application of described microbiobacterial agent in the water body of biological restoration petroleum pollution.
Rhodopseudomonas of the present invention (Pseudomonas sp) bacterial strain screens by a large amount of screening operations, and this bacterial strain has very strong degradation capability to petroleum hydrocarbon.This bacterial strain has been stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and (address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), preserving number was CGMCC No.4793 on 04 28th, 2011.Preserving number provided by the invention is that the Rhodopseudomonas (Pseudomonas sp) of CGMCC No.4793 and the microbiobacterial agent that contains this bacterium have very strong degradation capability to petroleum hydrocarbon, can be widely used in decomposing petroleum hydrocarbon and to the reparation of petroleum pollution, as to the reparation of the soil of petroleum pollution with to reparation of the water body of petroleum pollution etc.
Description of drawings
Fig. 1 has shown that the preserving number of the present invention's screening is that the Rhodopseudomonas (Pseudomonas sp) of CGMCC No.4793 is cultivated the form of 24 hours on solid TSA substratum;
Fig. 2 shown the preserving number of the present invention's screening be CGMCC No.4793 Rhodopseudomonas (Pseudomonas sp) cultivate on solid TSA substratum 24 hours by lawn dyeing after under opticmicroscope oil mirror the form of (10 * 100 times).
Embodiment
The invention provides a kind of Rhodopseudomonas (Pseudomonas sp), wherein, the deposit number of this Rhodopseudomonas (Pseudomonas sp) is CGMCC No.4793.Through 16s rDNA amplification and sequencing analysis, deposit number is that the bacterial strain of CGMCC No.4793 has the 16s rDNA sequence shown in SEQ ID NO:1.
The present invention also provides a kind of microbiobacterial agent of decomposing petroleum hydrocarbon, wherein, this microbiobacterial agent contains Rhodopseudomonas (Pseudomonas sp) as activeconstituents, and wherein, the deposit number of this Rhodopseudomonas (Pseudomonas sp) is CGMCC No.4793.The amount of the Rhodopseudomonas that contains in described microbiobacterial agent (Pseudomonas sp) can in very large range change, and under preferable case, the contained total viable count of the described microbiobacterial agent of every gram can be 0.1-2.5 * 10
10Individual, 0.5-1 * 10 more preferably
10Individual.
According to the present invention, described microbiobacterial agent also contains substratum, and described substratum is one or more in beef-protein medium, oil screening culture medium and PDA substratum, is preferably beef-protein medium.Above-mentioned substratum can be commercially available or prepare according to the record of " microbiological culture media handbook " (Microbiology Culture Media Manual).For example, described beef-protein medium can contain extractum carnis, peptone, NaCl and water, wherein, extractum carnis with respect to 100 weight parts, the content of peptone can be the 150-250 weight part, the content of NaCl can be the 50-150 weight part, and the content of water can be the 15000-25000 weight part, and the pH value can be 7.2-7.4; Add the agar of 1.2 % by weight to can be made into solid medium in substratum.
It is the Rhodopseudomonas (Pseudomonas sp) of CGMCC No.4793 and the application of microbiobacterial agent in decomposing petroleum hydrocarbon that contains this bacterial strain that the present invention also provides preserving number.
It is the Rhodopseudomonas (Pseudomonas sp) of CGMCC No.4793 and the application of microbiobacterial agent in the biological restoration petroleum pollution that contains this bacterial strain that the present invention also provides preserving number.
It is the Rhodopseudomonas (Pseudomonas sp) of CGMCC No.4793 and the application of microbiobacterial agent in the soil of biological restoration petroleum pollution that contains this bacterial strain that the present invention also provides preserving number.
It is the Rhodopseudomonas (Pseudomonas sp) of CGMCC No.4793 and the application of microbiobacterial agent in the water body of biological restoration petroleum pollution that contains this bacterial strain that the present invention also provides preserving number.
Below by specific embodiment, the present invention is carried out further description.
Experiment material
One, the oil sample of strain screening:
Pick up from the crude oil of Shengli Oil Field and commercially available 0# diesel oil and mix in the ratio of 1:4, as the experiment oil.
Two, pedotheque is the mixture of 1:1:1 that picks up from three kinds of different oil-polluted soils samples of Shengli Oil Field, Shandong, and it is respectively:
1) the ground crude oil contaminated soil around producing well;
2) oil sediment; With
3) stack for a long time the topsoil soil of oil sediment.
Three, substratum
1) enrichment medium: K
2HPO
43H
2O 1.0g, KH
2PO
41.0g, MgSO
47H
2O 0.5g, NH
4NO
31.0g, CaCl
20.02g distilled water 1000mL transfers to PH7.0,121 ℃ of sterilization 20min add the 5g oil after cooling.
2) isolation medium (PDA substratum): potato powder 6.0g, glucose 20g, agar 20g adds distilled water until 1000ml, and standby 115 ℃ of autoclavings 20 minutes.
3) strain store medium: beef-protein medium
4) screening culture medium (liquid): NaNO
31.5g, (NH4)
2SO
41.5g, K
2HPO
41g, MgSO
47H
2O 0.5g, KCl 0.5g, FeSO
47H
2O 0.01g, CaCl
20.002g distilled water 1000mL transfers to pH8.0,121 ℃ of sterilization 20min add the 2.5g oil after cooling.Add the agar of 1.2 % by weight to make the solid screening culture medium in substratum.
5) solid TSA substratum: Tryptones 15g, soya peptone 5g, sodium-chlor 30g, agar 15g adds distilled water until final volume is 1000ml, and the pH value is 7.1-7.5.
Embodiment 1
1, the concentration and separation of petroleum hydrocarbon degradation bacterial strain
Taking 5g oil-polluted soils mixture adds in the 250mL triangular flask that the 100mL enrichment medium is housed, cultivate under 28 ℃, the constant-temperature table condition of 180r/min, draw afterwards the 5mL nutrient solution in 5 days and be forwarded to fresh enrichment medium, the same terms was cultivated 5 days, enrichment culture is 25 days so continuously, nutrient solution is through being coated on after gradient dilution on solid PDA substratum, in 28 ℃ of cultivations; After flat board grew bacterium colony, the single bacterium colony of picking different shape adopted the line partition method repeatedly to carry out separation and purification, until obtain the pure growth of single strain, the bacterial strain after purifying is stored in the beef-protein medium inclined-plane.
2, the screening of high degrading activity bacterial strain
The bacterial strain of above-mentioned purifying is inoculated into respectively in the 250mL triangular flask that the 100mL screening culture medium is housed, cultivated 5 days under 28 ℃, the condition of 180r/min, after being diluted 100 times, nutrient solution is coated on the solid screening culture medium, observe the growing state of each bacterial strain in solid medium, filter out grow very fast, the more bacterial strain of bacterium colony.
Single bacterium colony of each bacterial strain of picking is inoculated into respectively in the 250ml triangular flask that 50ml liquid beef-protein medium is housed, cultivate 24h in 28 ℃, the constant-temperature table of 180r/min, measure bacterium liquid in the absorbancy at 600nm place, regulate its mass concentration with the beef-protein medium of sterilization and make OD600nm be about 0.4, make seed inoculation screening culture medium with this bacterium liquid that dilutes.
Drawing the ready seed liquor of 5ml is inoculated in the 250mL triangular flask that the 100mL screening culture medium is housed, measure oil content cultivate 4 days in 28 ℃, the constant-temperature table of 180r/min after, the processing triplicate of each seed liquor, calculate average degradation rate, filter out the highest bacterial strain of petroleum degradation rate, and this bacterial strain has been carried out biological preservation, preserving number is CGMCC No.4793.
Embodiment 2
According to " experiment content and experimental technique of record in common bacteria system identification handbook [4] and " actinomycetes systematic theory, method and put into practice " [5], to sieve preserving number be that the bacterial strain of CGMCC No.4793 carries out strain identification.
1, morphological analysis
The preserving number bacterial strain that is CGMCC No.4793 is carried out morphological analysis and at the optical microphotograph Microscopic observation, result respectively as illustrated in fig. 1 and 2.
Colonial morphology is observed and is carried out on solid TSA substratum, after the bacterial strain streak inoculation, observes colonial morphology in 24 hours in 28 ℃ of constant temperature culture.Preserving number is that the bacterium colony of the bacterial strain of CGMCC No.4793 is colourless translucent, circle, and the edge is irregular, smooth texture, surface flat.Picking is cultivated 24 hours rear 100 times of oily Microscopic observations at opticmicroscope of lawn dyeing.Can clearly see at microscopically, preserving number is that the somatic cells of the bacterial strain of CGMCC No.4793 is stock shape, and single appearance.
2,16s rDNA identifies
The pcr amplification of 16s rDNA and order-checking: the purifying preserving number is total DNA of the bacterial classification of CGMCC No.4793 to adopt test kit (Beijing full Shi Jin biotech company) to extract also, then take total DNA as template, 16s rDNA conserved sequence is carried out pcr amplification obtain the goal gene fragment.
The pcr amplification primer is respectively:
27f(5 '-AGAGTTTGATCCTGGCTCAG-3 ') and;
1492r（5’-TACGGCTACCTTGTTACGACTT-3’）。
PCR total reaction system is 50uL, specifically comprises:
| 10×Buffer | 5μL |
| 10mmol/L dNTP | 4μL |
| Reverse primer | 1μL |
| Forward primer | 1μL |
| Taq enzyme (5U/ μ L) | 1μL |
| Template DNA | 2μL |
| ddH 2O | Complement to 50 μ L |
The PCR reaction conditions is: 94 ℃ of denaturation 5min, and 94 ℃ of sex change 1min, 50 ℃ of annealing 1min, 72 ℃ are extended 2min, and this step is carried out 35 circulations altogether, and last 72 ℃ are extended 10min.
Amplified production carries out the electrophoresis check with 1% sepharose.The examining order of PCR product is completed by Beijing three rich polygala root biotech company.The sequencing result demonstration, preserving number is that the bacterial strain of CGMCC No.4793 has the 16s rDNA sequence shown in SEQ ID NO:1.
Through Phylogenetic Analysis: log in http://www.ncbi.nlm.nih.gov, check order row are analyzed by the sequence in blast program and nucleic acid database search same or analogous nucleotide sequence, then utilize sequence alignment software ClustalX 2.0 and Phylogenetic Analysis software MEGA 4.0.2 constructing system evolutionary tree.In MEGA software, the method of constructing system evolutionary tree is used the Neighbor-Joining method, the base alternative model is selected the Kimura two-parameter model, the evolutionary tree certificate authenticity is selected 1000 duplicate detection of check (bootstrap) of bootstrapping, and identical weighted value is given in the conversion in mutant dna sequence and transversion.Show by analysis, preserving number is that the bacterial strain of CGMCC No.4793 belongs to Rhodopseudomonas (Pseudomonas sp).
Embodiment 3
Taking 10g oil-polluted soils mixture (oil content 1.25 grams) adds and the 100mL beef-protein medium is housed (preserving number is that the inoculum size of the pseudomonas of CGMCC No.4793 is that the contained total viable count of every gram substratum is 0.5 * 10
10Individual) the 250mL triangular flask in, cultivate under 28 ℃, the constant-temperature table condition of 180r/min, detected afterwards the oil content in the oil-polluted soils mixture in 3 days, result is 0.51 gram, this bacterial strain is about 59.2% to the degradation rate of oil.
Embodiment 4
Taking 10g oil-polluted soils mixture (oil content 1.25 grams) adds and the 100mL beef-protein medium is housed (preserving number is that the inoculum size of the pseudomonas of CGMCC No.4793 is that the contained total viable count of every gram substratum is 0.8 * 10
10Individual) the 250mL triangular flask in, cultivate under 28 ℃, the constant-temperature table condition of 180r/min, detected afterwards the oil content in the oil-polluted soils mixture in 3 days, result is 0.45 gram, this bacterial strain is about 64% to the degradation rate of oil.
Embodiment 5
Taking 10g oil-polluted soils mixture (oil content 1.25 grams) adds and the 100mL beef-protein medium is housed (preserving number is that the inoculum size of the pseudomonas of CGMCC No.4793 is that the contained total viable count of every gram substratum is 0.3 * 10
10Individual) the 250mL triangular flask in, cultivate under 28 ℃, the constant-temperature table condition of 180r/min, detected afterwards the oil content in the oil-polluted soils mixture in 3 days, result is 0.55 gram, this bacterial strain is about 56% to the degradation rate of oil.
Can be found out by the above results, the preserving number that the present invention filters out is that the bacterial strain of CGMCC No.4793 has good degradation capability to petroleum hydrocarbon, can be widely used in decomposing petroleum hydrocarbon and to the reparation of petroleum pollution, as to the reparation of the soil of petroleum pollution with to reparation of the water body of petroleum pollution etc.
SEQUENCE LISTING
＜110〉Institute of Environment and Sustainable Development in Agriculture, CAAS
＜120〉a kind of Rhodopseudomonas and microbiobacterial agent and their application
<160> 1
<170> PatentIn version 3.4
<210> 1
<211> 1414
<212> DNA
＜213〉Rhodopseudomonas (
Pseudomonas) 16s rDNA
<400> 1
gctacacatg caagtcgagc ggatgaaggg agcttgctcc tggattcagc ggcggacggg 60
tgagtaatgc ctaggaatct gcctggtagt gggggataac gttccgaaag gaacgctaat 120
accgcgtacg tcctacggga gaaagcaggg gaccttcggg ccttgcgcta tcagatgagc 180
ctaggtcgga ttagctagtt ggtgaggtaa tggctcacca aggcgacgat ccgtaactgg 240
tctgagagga tgatcagtca cactggaact gagacacggt ccagactcct acgggaggca 300
gcagtgggga atattggaca atgggcgaaa gcctgatcca gccatgccgc gtgtgtgaag 360
aaggtcttcg gattgtaaag cactttaagt tgggaggaag ggctgtaggc taatatcctg 420
cggttttgac gttaccaaca gaataagcac cggctaactt cgtgccagca gccgcggtaa 480
tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttcgt 540
taagttggat gtgaaagccc cgggctcaac ctgggaactg catccaaaac tggcgagcta 600
gagtacggta gagggtagtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag 660
gaacaccagt ggcgaaggcg actacctgga ctgatactga cactgaggtg cgaaagcgtg 720
gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgtc aactagccgt 780
tggaatcctt gagattttag tggcgcagct aacgcattaa gttgaccgcc tggggagtac 840
ggccgcaagg ttaaaactca aatgaattga cgggggcccg cacaagcggt ggagcatgtg 900
gtttaattcg aagcaacgcg aagaacctta cctggccttg acatgctgag aactttccag 960
agatggattg gtgccttcgg gaactcagac acaggtgctg catggctgtc gtcagctcgt 1020
gtcgtgagat gttgggttaa gtcccgtaac gagcgcaacc cttgtcctta gttaccagca 1080
cgttatggtg ggcactctaa ggagactgcc ggtgacaaac cggaggaagg tggggatgac 1140
gtcaagtcat catggccctt acggccaggg ctacacacgt gctacaatgg tcggtacaaa 1200
gggttgccaa gccgcgaggt ggagctaatc ccataaaacc gatcgtagtc cggatcgcag 1260
tctgcaactc gactgcgtga agtcggaatc gctagtaatc gtgaatcaga atgtcacggt 1320
gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgggagtgg gttgctccag 1380
aagtagctag tctaaccttc ggggggacgg tacc 1414
Claims (10)
1. a Rhodopseudomonas (Pseudomonas sp), is characterized in that, the deposit number of this Rhodopseudomonas (Pseudomonas sp) is CGMCC No.4793.
2. the microbiobacterial agent of a decomposing petroleum hydrocarbon, is characterized in that, this microbiobacterial agent contains Rhodopseudomonas (Pseudomonas sp) as activeconstituents, and wherein, the deposit number of this Rhodopseudomonas (Pseudomonas sp) is CGMCC No.4793.
3. microbiobacterial agent according to claim 2, wherein, the contained total viable count of the described microbiobacterial agent of every gram is 0.1-2.5 * 10
10Individual.
4. microbiobacterial agent according to claim 3, wherein, the contained total viable count of the described microbiobacterial agent of every gram is 0.5-1 * 10
10Individual.
5. the described microbiobacterial agent of any one according to claim 2-4, wherein, this microbiobacterial agent also contains substratum, and described substratum is one or more in beef-protein medium, oil screening culture medium and PDA substratum.
6. microbiobacterial agent according to claim 5, wherein, described substratum is beef-protein medium.
7. the application of the described microbiobacterial agent of any one in decomposing petroleum hydrocarbon in Rhodopseudomonas claimed in claim 1 or claim 2-6.
8. the application of the described microbiobacterial agent of any one in the biological restoration petroleum pollution in Rhodopseudomonas claimed in claim 1 or claim 2-6.
9. the application of the described microbiobacterial agent of any one in the soil of biological restoration petroleum pollution in Rhodopseudomonas claimed in claim 1 or claim 2-6.
10. the application of the described microbiobacterial agent of any one in the water body of biological restoration petroleum pollution in Rhodopseudomonas claimed in claim 1 or claim 2-6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110167206 CN102250798B (en) | 2011-06-21 | 2011-06-21 | Pseudomonas and microbial agent as well as applications thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110167206 CN102250798B (en) | 2011-06-21 | 2011-06-21 | Pseudomonas and microbial agent as well as applications thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102250798A CN102250798A (en) | 2011-11-23 |
| CN102250798B true CN102250798B (en) | 2013-11-06 |
Family
ID=44978370
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110167206 Expired - Fee Related CN102250798B (en) | 2011-06-21 | 2011-06-21 | Pseudomonas and microbial agent as well as applications thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102250798B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108034625A (en) * | 2018-02-06 | 2018-05-15 | 北京大学 | A kind of degradation bacteria strains JN7 of oily sludge petrochina hydro carbons and its application |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937724B (en) * | 2014-04-24 | 2016-10-19 | 烟台大境生态环境科技股份有限公司 | Yellow sea pseudomonas and microbial bacterial agent and their application |
| CN104745506A (en) * | 2015-03-11 | 2015-07-01 | 河南省水文地质工程地质勘察院有限公司 | Petroleum hydrocarbon degrading bacteria and application thereof |
| CN104830723B (en) * | 2015-04-30 | 2018-04-20 | 大连民族学院 | There is the bacterium AW25 of oil degradation and its purposes and bacteria carrier |
| CN106754528B (en) * | 2016-12-29 | 2020-09-29 | 长安大学 | Pseudomonas flexi and application thereof |
| EP3456730B1 (en) * | 2017-09-19 | 2022-03-02 | BRAIN Biotech AG | A new species of the genus pseudomonas |
| CN108102979B (en) * | 2018-02-06 | 2020-08-04 | 北京大学 | Degradation strain JN5 for petroleum hydrocarbons in oily sludge and application thereof |
| CN108048374B (en) * | 2018-02-06 | 2020-08-04 | 北京大学 | Degradation strain JN4 for petroleum hydrocarbons in oily sludge and application thereof |
| CN109593687A (en) * | 2019-01-21 | 2019-04-09 | 杭州造品科技有限公司 | One pseudomonas and its application in oil degradation |
| CN110055187B (en) * | 2019-02-28 | 2021-01-08 | 中国农业科学院农业环境与可持续发展研究所 | Pseudomonas proteorum and application thereof |
| CN110564654A (en) * | 2019-10-12 | 2019-12-13 | 江苏圣泰环境科技股份有限公司 | Microbial inoculum for repairing petroleum-polluted soil |
| CN113980856B (en) * | 2021-11-17 | 2023-08-29 | 中国农业科学院研究生院 | New pseudomonas strain and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007215403A (en) * | 2005-11-21 | 2007-08-30 | Asahi Breweries Ltd | Method for decomposing environmental pollutant by using microorganism |
| CN101486980A (en) * | 2009-02-16 | 2009-07-22 | 于洋 | Solid microbial preparation for petroleum pollutant and oil product degradation, preparation and use |
-
2011
- 2011-06-21 CN CN 201110167206 patent/CN102250798B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007215403A (en) * | 2005-11-21 | 2007-08-30 | Asahi Breweries Ltd | Method for decomposing environmental pollutant by using microorganism |
| CN101486980A (en) * | 2009-02-16 | 2009-07-22 | 于洋 | Solid microbial preparation for petroleum pollutant and oil product degradation, preparation and use |
Non-Patent Citations (6)
| Title |
|---|
| "Investigation of saturated and aromatic hydrocarbon resistance mechanisms in Pseudomonas aeruginosa IBBML1";Lazaroaie, MM;《CENTRAL EUROPEAN JOURNAL OF BIOLOGY》;20091231;第4卷(第4期);1-10 * |
| "石油烃降解菌的筛选与鉴定";崔丽虹;《生物技术通报》;20090930(第9期);143-147 * |
| Lazaroaie, MM."Investigation of saturated and aromatic hydrocarbon resistance mechanisms in Pseudomonas aeruginosa IBBML1".《CENTRAL EUROPEAN JOURNAL OF BIOLOGY》.2009,第4卷(第4期), |
| 崔丽虹."石油烃降解菌的筛选、鉴定及复合菌群降解效果的研究".《中国优秀硕士学位论文全文数据库（工程科技Ⅰ辑）》.2009,(第10期), |
| 崔丽虹."石油烃降解菌的筛选与鉴定".《生物技术通报》.2009,(第9期), |
| 崔丽虹."石油烃降解菌的筛选、鉴定及复合菌群降解效果的研究".《中国优秀硕士学位论文全文数据库(工程科技Ⅰ辑)》.2009,(第10期), * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108034625A (en) * | 2018-02-06 | 2018-05-15 | 北京大学 | A kind of degradation bacteria strains JN7 of oily sludge petrochina hydro carbons and its application |
| CN108034625B (en) * | 2018-02-06 | 2020-08-04 | 北京大学 | Degradation strain JN7 for petroleum hydrocarbons in oily sludge and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102250798A (en) | 2011-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102250798B (en) | Pseudomonas and microbial agent as well as applications thereof | |
| CN102250794B (en) | Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof | |
| CN102250801B (en) | Microbial agent, and its preparation method and application | |
| CN102533601B (en) | Bacillus simplex, and culture method and application thereof | |
| CN109182178B (en) | Strain with chromium tolerance and Cr (VI) removal capacity and application thereof in-situ remediation of moderately and slightly chromium-polluted soil | |
| CN106167776A (en) | A kind of can bacillus cereus (Bacillus cereus) TH 35 of heavy metal cadmium and application thereof in activating soil | |
| CN102399719B (en) | Bacterium DW3 capable of degrading marine diesel oil pollutants | |
| Wu et al. | A rock-weathering bacterium isolated from rock surface and its role in ecological restoration on exposed carbonate rocks | |
| CN110217895A (en) | A kind of complex micro organism fungicide and its application for water environment treatment | |
| CN102250797B (en) | Gordoniaamicalis and microbial agent and applications thereof | |
| CN104087525A (en) | Novel seawater fermentation strain generating biosurfactant | |
| CN102250770B (en) | Microbial agent and preparation method and application thereof | |
| CN108300674A (en) | A kind of oil degradation bacteria and its preparation method and the application in degrading crude oil | |
| CN102250796B (en) | Rhodococcusruber and microbial agent and application of Rhodococcusruber and microbial agent | |
| Barua et al. | Studies on non-symbiotic diazotrophic bacterial populations of coastal arable saline soils of India | |
| CN102250795B (en) | Gordoniaalkanivorans, microbial agent and use thereof | |
| CN114107092A (en) | Plant endophyte Gordonia L191 for degrading phthalate and application thereof | |
| CN102492637A (en) | Atrazine degrading bacterium | |
| CN104726370A (en) | Enterobacter with crude oil degradation effect and application of enteric bacilli | |
| CN102382784A (en) | Salt-resistant Serratiasp. strain and application thereof in restoration of salinized petroleum-contaminated soil | |
| CN104845899A (en) | Application of Rhodococcus sp. 2G in degradation of phthalate | |
| CN106591173A (en) | Bacillus flexus HL-37 capable of activating soil heavy metal cadmium, and applications thereof | |
| CN104805018A (en) | Agromyces sp. MT-E used for simultaneous degradation of plurality of phthalic acid esters | |
| CN104845890A (en) | Application of Agromyces sp. MT-E in degradation of various phthalates | |
| CN113897320A (en) | Halophyte with salt-tolerant growth-promoting function and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131106 Termination date: 20200621 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |