CN102250783A - Enrichment culturing method for ammonia oxidizing bacteria - Google Patents
Enrichment culturing method for ammonia oxidizing bacteria Download PDFInfo
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- CN102250783A CN102250783A CN2010102666767A CN201010266676A CN102250783A CN 102250783 A CN102250783 A CN 102250783A CN 2010102666767 A CN2010102666767 A CN 2010102666767A CN 201010266676 A CN201010266676 A CN 201010266676A CN 102250783 A CN102250783 A CN 102250783A
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- ammonia oxidizing
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- oxidizing bacteria
- nitrobacteria
- culturing
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Abstract
The invention discloses an enrichment culturing method for ammonia oxidizing bacteria. The method comprises the following steps: adding activated sludge containing nitrobacteria into a culture medium which takes landfill leachate as a raw material; controlling the concentration of DO (dissolved oxygen) to be between 2 and 3mg/L; keeping constant shading aeration in a culturing process; and taking 5 days as a culture cycle for one generation, and culturing 4 generations in total. Experimental comparisons prove that the ammonia oxidizing efficiency of the ammonia oxidizing bacteria, cultured in the landfill leachate, is increased by 20.9 percent compared with that of the ammonia oxidizing bacteria not cultured in the landfill leachate. The economical and efficient nitrobacterium with high concentration can be prepared by the method, and the prepared high-concentration nitrobacterium is added into a waste water biological denitrification process so as to improve the denitrification efficiency, so that the processing time is greatly shortened, and unnecessary engineering cost is reduced, thereby developing a novel denitrification thought for the denitrification process of a sewage treatment plant.
Description
Technical field
The present invention relates to the environmental microorganism field, relate in particular to a kind of enrichment culture method of handling the ammonia oxidation bacteria of nitrogen in the waste water.
Background technology
Since nitrogen to hydrobiont circle eutrophication have a strong impact on and to hydrobiological toxicity, denitrogenation of waste water more and more is subjected to people's attention.The cost-effective method that from waste water, removes nitrogen commonly used be nitrated/return nitrofication process.But because nitrifier is an autotrophic bacteria, its poor growth, and very easily suppressed by toxic substance, so, how to guarantee effectively that the treatment unit for waste water nitric efficiency becomes the problem that needs solve.Recover nitrification activity in the nitrifier short period of time but add, shorten stoppage time, guarantee the waste water water quality qualified discharge that dispatches from the factory.But nitrated microbial inoculum costliness, and be difficult for preserving.
Percolated liquid in city refuse landfill is a kind of complicated component, and ammonia nitrogen concentration height, C/N directly do not enter environment than low high concentrated organic wastewater if do not add processing, can cause serious environmental to pollute.The processing of percolate ammonia nitrogen is the problem of making us very much " headache " always, and domestic a lot of percolate from garbage filling field are handled can not be up to standard, is because this index of ammonia nitrogen can't be up to standard to a great extent.
Summary of the invention
Problem based on above-mentioned prior art exists the objective of the invention is to utilize this convenience of high ammonia nitrogen in the percolate to provide a kind of cost-effective method to obtain the nitrifier of high density, thereby is used for the denitrogenation processing of waste water.
The present invention realizes by the following method:
The active sludge that will contain nitrobacteria joins to cultivate in the substratum of percolate as raw material, and incubation time was a generation culture cycle with 5 days, cultivates through 4 cycles of supporting of being commissioned to train altogether.
Further, in culturing process, control dissolved oxygen (DO) concentration is at 2～3mg/L.
Further, described substratum add active sludge after the SS value be 2000～3500mg/L.
Further, in culturing process, keep continuing aeration.
Further, in above-mentioned culturing process, keep omnidistance shading to cultivate.
The step of the specific implementation of this programme can be as follows:
1, the outfit of raw material
A. substratum: the percolate of taking from city life garbage landfill
Table 1 percolate water quality situation
| NH4+-N(N,mg/L) | COD(O 2,mg/L) | SS(mg/L) | PH |
| 1440 | 4.31×103 | 109 | 9.18 |
B. artificial distribution's water quality
Table 2 artificial distribution water quality situation
| KH 2PO 4 | Na 2HPO 4 | (NH 4) 2SO 4 | CaCl 2·2H 2O | MgSO 4·7H 2O | NaHCO 3 |
| 0.7g/L | 13.5g/L | 2.5g/L | 0.005g/L | 0.01g/L | 0.5g/L |
C. active sludge
Take from the returned sluge of sewage work, its activated sludge concentration of rough determination (SS) value is 10248mg/L.Before the experiment beginning, carry out aeration to this active sludge and cultivate, make it keep active.
D. experimental installation
Adopt the triangular flask of 1L that nitrobacteria is carried out the aeration cultivation, during enrichment culture, triangular flask is outer will be wrapped with newspaper, and to reach the lucifuge requirement, simultaneously, triangular flask is suitable for reading will to add tampon, to stop the invasion of assorted bacterium.
2, the enrichment culture of nitrobacteria
The active sludge that will contain the nitrobacteria bacterial classification joins in the substratum, control DO concentration is at 2～3mg/L, measure ammonia nitrogen concentration every day one time, and record temperature, DO and pH value, cultivated through 5 days, the first-generation is cultivated and is finished, and adds 2 times of first-generation to it again and cultivates the long-pending percolate of residue bacteria liquid, carries out the s-generation and cultivates.Using such method is carried out the enrichment culture of the nitrobacteria in four generations.
Beneficial effect of the present invention is to utilize and is rich in high ammonia nitrogen in the percolate and comes the enrichment culture nitrobacteria, both can effectively solve percolate discharging problem not up to standard, nitrobacteria that again can cost-effective acquisition high density, and can improve nitric efficiency by the method that in wastewater biological denitrificaion technology, adds the nitrifier liquid of denseization, thereby shorten the process time greatly, reduce unnecessary construction costs, for the denitrification process of sewage work is opened up new denitrogenation thinking, can alleviate nitrogen effectively to natural pollution simultaneously.
Description of drawings
Fig. 1 is the ammonia nitrogen concentration change curve of parallel sample of the present invention in 1.;
Fig. 2 is the ammonia nitrogen concentration change curve of parallel sample of the present invention in 2.;
Fig. 3 is the interior ammonia nitrogen concentration of an A reactor of the present invention variation diagram in time;
Fig. 4 is the interior ammonia nitrogen concentration of a B reactor of the present invention variation diagram in time.
Embodiment
For ease of understanding,, the specific embodiment of the present invention is described further below by specific embodiment.
The active checking of embodiment 1 active sludge
The returned sluge of fetching is inoculated among the artificial distribution, inoculation back SS value is maintained about 2000-3500mg/L, a good living environment is arranged to guarantee nitrobacteria.Next every 3～4 hours mensuration ammonia nitrogen concentration wherein, measure by 4 times ammonia nitrogen concentrations, find that ammonia nitrogen concentration is on a declining curve, illustrate that thus active sludge is activated, be that nitrobacteria still survives in the active sludge, can be as the bacterial classification source of nitrobacteria.
The enrichment culture of embodiment 2 nitrobacterias
Get the triangular flask of two 1L, respectively get the percolate of 550mL and make substratum, each is to wherein inserting the active sludge that 250mLSS concentration is 10248mg/L then, mix the back and measure the interior SS concentration of two reactors all about 2300mg/L, measure ammonia nitrogen concentration and chemical oxygen demand (COD) (COD) value in two reactors simultaneously, and record temperature, DO, and pH value, and make DO concentration maintain 2～3mg/L.
After on-test, measure ammonia nitrogen concentration every day one time, and record temperature, DO and pH value.Through 5 days cultivation, ammonia nitrogen concentration was dropped to below the 100mg/L by the 800mg/L (in N) in when beginning, and so far, the first-generation is cultivated and finished.When beginning and finish, all needs in per generation SS, the COD of the bacterium liquid in the reactor are measured.The first-generation is cultivated when finishing, and last bacterium liquid is few in the reactor, so cultivate the long-pending percolate of residue bacteria liquid to wherein adding 2 times of first-generation, carries out the s-generation and cultivates.Using such method has been carried out the enrichment culture of the nitrobacteria of four times in generation.Because each ammonia nitrogen concentration of surveying when COD and SS value, all has the part bacterium to be removed with liquid, so when finishing to cultivate, there is not much left for the interior active sludge of discovery reactor.Fig. 1, Fig. 2 be respectively during the enrichment culture above-mentioned two parallel samples 1., 2. in the change curve of ammonia nitrogen concentration.
Concrete verification experimental verification
The checking of experimental example 1 rate of nitrification
Other gets the triangular flask of two 1L, and label is A and B respectively, according to add corresponding solution shown in the following table 3 in two triangular flasks of described A, B.
Table 3: the proof test prescription of rate of nitrification
Annotate: 1. cultivate the residue bacterium liquid in 1. of parallel sample after four generations for above-mentioned enrichment culture nitrobacteria
Cultivate nitrobacteria according to above-mentioned prescription, continuous aeration was cultivated 8 hours, and the method for cultural method during with the nitrobacteria enrichment culture is consistent, does not just need the replacement.Surveyed an ammonia nitrogen concentration every 2 two hours, observe the variation of ammonia nitrogen concentration in the even individual reactor of A, B, when beginning, centre and end, respectively survey the SS value one time simultaneously, so that the rate of nitrification of the nitrobacteria of final Units of Account SS quality.
Experimental example 2
By Fig. 1 and Fig. 2 as can be seen, through the enrichment culture of four times in generation, per generation ammonia nitrogen concentration all present downtrending, can determine in whole enrichment culture process, nitrobacteria is in active survival condition, shows that it is feasible utilizing percolate to cultivate nitrobacteria.To have obtained enrichment in order verifying, to have carried out the contrast experiment of ammonia oxidation efficient through ammonia oxidation bacteria in 4 mud of being commissioned to train after supporting.
Confirmatory experiment by rate of nitrification can get Fig. 3 and Fig. 4, and the slope of figure cathetus is respectively 20.3mgN/h and 15.9mgN/h.Fig. 3 represents that the A reactor has added that the ammonia oxidation efficient of nitrobacteria is 9.08mgNH4+-N/ (gSSh) behind the enrichment culture bacterium liquid, and Fig. 4 represents that the ammonia oxidation efficient of nitrobacteria in the B reactor after nitrobacteria in the B reactor ordinary activated sludge adds percolate is 7.19mgNH4+-N/ (gSSh).By comparing both ammonia oxidation efficient of A, B as can be known, in active sludge, add 14.5% ammonia oxidation bacteria through the acquisition of percolate enrichment culture, keeping under the constant prerequisite of total suspended particles concentration, ammonia oxidation efficient has improved 20.9%, the ammonia oxidation bacteria through the percolate enrichment culture of interpolation is given the credit in the raising that shows ammonia oxidation efficient, (ammonia oxidation bacteria of supposition enrichment is identical with the ammonia oxidation bacteria activity in the active sludge) as calculated, it is foster to be commissioned to train through 4, and the concentration of the ammonia oxidation bacteria of enrichment is 2.9 times of ammonia oxidation bacteria concentration in the active sludge.
Therefore it is very cost-effective utilizing percolate enrichment culture ammonia oxidation bacteria.
The above; only for the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, and anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (5)
1. the enrichment culture method of an ammonia oxidation bacteria comprises that the active sludge that will contain nitrobacteria joins to cultivate in the substratum of percolate as raw material, and incubation time was a generation culture cycle with 5 days, cultivates through 4 cycles of supporting of being commissioned to train altogether.
2. cultural method according to claim 1 is characterized in that: in culturing process, control DO concentration is at 2～3mg/L.
3. cultural method according to claim 1 is characterized in that: the SS value behind the described substratum adding active sludge is 2000～3500mg/L.
4. according to each described cultural method of claim 1 to 3, it is characterized in that: in culturing process, keep continuing aeration.
5. cultural method according to claim 4 is characterized in that: in culturing process, keep omnidistance shading to cultivate.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288223A (en) * | 2013-06-09 | 2013-09-11 | 北京工业大学 | Ammonia oxidizing bacterium enriching device and method suitable for purifying micro-polluted water and application of ammonia oxidizing bacterium enriching device and method |
| CN103396966A (en) * | 2013-08-09 | 2013-11-20 | 湖北科亮生物环保科技有限公司 | Method for producing high-efficiency composite autotrophic nitrifying bacteria |
| CN104593304A (en) * | 2015-01-21 | 2015-05-06 | 浙江大学 | Quick enrichment culturing method of ocean ammoxidation archaea |
| CN106517539A (en) * | 2016-11-28 | 2017-03-22 | 北京工业大学 | Method for directionally quick screening and enrichment of broad-spectrum ammonia oxidizing bacteria |
| CN111349577A (en) * | 2018-12-21 | 2020-06-30 | 中国石油化工股份有限公司 | Culture method of ammonia oxidizing bacteria |
| CN113186146A (en) * | 2021-05-13 | 2021-07-30 | 深高蓝德环保科技集团股份有限公司 | Method for culturing microbial agent by using town landfill leachate |
| CN113308393A (en) * | 2021-04-20 | 2021-08-27 | 上田环境修复有限公司 | Preparation for degrading ammonia nitrogen and total nitrogen and preparation method thereof |
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| CN101164926A (en) * | 2007-08-17 | 2008-04-23 | 浙江大学 | Domestic garbage stuffing and processing technique with in-situ denitrogenation function and bioreactor |
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| CN101164926A (en) * | 2007-08-17 | 2008-04-23 | 浙江大学 | Domestic garbage stuffing and processing technique with in-situ denitrogenation function and bioreactor |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288223A (en) * | 2013-06-09 | 2013-09-11 | 北京工业大学 | Ammonia oxidizing bacterium enriching device and method suitable for purifying micro-polluted water and application of ammonia oxidizing bacterium enriching device and method |
| CN103396966A (en) * | 2013-08-09 | 2013-11-20 | 湖北科亮生物环保科技有限公司 | Method for producing high-efficiency composite autotrophic nitrifying bacteria |
| CN104593304A (en) * | 2015-01-21 | 2015-05-06 | 浙江大学 | Quick enrichment culturing method of ocean ammoxidation archaea |
| CN106517539A (en) * | 2016-11-28 | 2017-03-22 | 北京工业大学 | Method for directionally quick screening and enrichment of broad-spectrum ammonia oxidizing bacteria |
| CN106517539B (en) * | 2016-11-28 | 2019-06-14 | 北京工业大学 | The orientation method that quickly screening is enriched with broad spectrum activity ammonia oxidizing bacteria |
| CN111349577A (en) * | 2018-12-21 | 2020-06-30 | 中国石油化工股份有限公司 | Culture method of ammonia oxidizing bacteria |
| CN111349577B (en) * | 2018-12-21 | 2022-07-08 | 中国石油化工股份有限公司 | Culture method of ammonia oxidizing bacteria |
| CN113308393A (en) * | 2021-04-20 | 2021-08-27 | 上田环境修复有限公司 | Preparation for degrading ammonia nitrogen and total nitrogen and preparation method thereof |
| CN113186146A (en) * | 2021-05-13 | 2021-07-30 | 深高蓝德环保科技集团股份有限公司 | Method for culturing microbial agent by using town landfill leachate |
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