CN102249894B - Method for filtering and clarifying citric acid fermentation broth - Google Patents
Method for filtering and clarifying citric acid fermentation broth Download PDFInfo
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- CN102249894B CN102249894B CN201010583693.3A CN201010583693A CN102249894B CN 102249894 B CN102249894 B CN 102249894B CN 201010583693 A CN201010583693 A CN 201010583693A CN 102249894 B CN102249894 B CN 102249894B
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Abstract
The invention relates to a method for filtering and clarifying a citric acid fermentation broth. The method is characterized by the steps that: before the citric acid fermentation broth is processed, various flocculating agents and filter aids are added to feed liquid; filtering is then carried out, such that the clarified fermentation broth with a turbidity lower than 5 is obtained. With the method provided by the invention, quality of the fermentation broth is substantially improved. Not only the turbidity of the fermentation broth is substantially reduced, but the contents of internal colloids, proteins and other impurities in the fermentation broth are also reduced. Therefore, a burden on post extraction is greatly reduced.
Description
Technical field
The present invention relates to biological chemical field, be specifically related to a kind of citric acid fermentation broth and filter defecation method.
Background technology
Citric acid is first acid in organic acid, due to the performance of physicals, chemical property, derivative, is widely used in the industries such as food, medicine, daily use chemicals.In suitability for industrialized production, citric acid is mainly to take the starchy materials such as corn, cassava through submerged fermentation, to make as raw material.After fermentation ends, can obtain a large amount of citric acid fermentation wine with dregs, wherein contain many impurity, as mycelium, albumen, colloid, pigment etc., as without purifying and filtration treatment, will be difficult to directly enter rear extraction workshop section.At present, general way is karusen to be carried out to initial filter and multiple filter is processed, and the clear liquid of acquisition is transported to rear extraction workshop section again.The shortcoming of this technique was that cleaner liquid is of poor quality, unstable, its turbidity is conventionally more than 10, and the materials such as the larger albumen of rear extraction impact, colloid are effectively removed, cause rear extraction passive, every unit consumption index is obviously higher, the byproduct calcium sulfate color obtaining is poor, is unfavorable for follow-up comprehensive recycling.
Although disclose a kind for the treatment of process of citric acid mother liquor in the patent that is 200710113693.5 in the patent No., the impurity such as colloid are wherein effectively removed, obtain good turbidity, but had essence different from the present invention, cannot be applied to the processing of citric acid fermentation broth.Compare with citric acid mother liquor, citric acid fermentation broth composition is more complicated, amount is large, process more difficult, processing cost is high, therefore, need to find a kind of effective filtering fermentation liquor defecation method, from feed liquid source, do, to improve the shortcoming that fermented liquid turbidity is poor, protein-colloid content is high, thereby greatly alleviate the facility load of subsequent disposal, significantly reduce production run cost.
Summary of the invention
For above existing problems, the present invention proposes a kind of filtration defecation method of citric acid fermentation broth, adopt present method, can be effective improve the shortcoming that fermented liquid turbidity is poor, protein-colloid content is high, obtain high-quality citric acid fermentation broth.
Key step is as follows:
1) coagulation
First in citric acid fermentation broth, add flocculation agent to stir, then add flocculating aids to stir, carry out solid-liquid separation;
2) second-stage treatment
In above-mentioned filtrate, add flocculation agent to stir, then add flocculating aids to stir, carry out solid-liquid separation;
The flocculation agent adding in described coagulation is different from the flocculation agent adding in second-stage treatment and flocculating aids with flocculating aids, and its concrete technology step is as follows:
(1), in citric acid fermentation broth, add flocculation agent and stir, adding proportion is 0.1-2g/m
3;
(2), to citric acid fermentation broth, add flocculating aids and stir, adding proportion is 0.1-2kg/m
3;
(3), above-mentioned mixed solution is carried out to one-level solid-liquid separation with plate-and-frame filter press;
(4), in the filtrate after filtering, again add flocculation agent and stir, adding proportion is 0.1-1g/m
3;
(5), in the filtrate after filtering, again add flocculating aids and stir, adding proportion is 0.1-2kg/m
3;
(6), above-mentioned mixed solution is carried out to secondary solid-liquid separation with plate-and-frame filter press.
In step (1), by test of many times, prove that the flocculating effect of polyacrylamide or sodium polyacrylate is best, they can with fermented liquid in soluble proteins, colloid flocculate, thereby it is precipitated out.Table 1 is flocculation agent kind and the impact of adding proportion on colloid protein content thereof.By table 1, drawn, when the adding proportion of polyacrylamide/sodium polyacrylate is 0.1-2g/m
3time, flocculating effect is better, not only can make albumen and colloid fully flocculate but also can guarantee that flocculation agent can not remain in fermented liquid, the mass formation impact on citric acid fermentation broth.
Table 1 flocculation agent kind and adding proportion thereof and colloid protein content relation
In step (2), adding flocculating aids is the flocs unit surface formation drainage coating in order to produce in step (1), and flocs unit is disperseed, and is easy to filter.Because this is filtered into single filter, relatively low to the requirement of filtrate turbidity, so select the flocculating aids of relative low price.Table 2 is flocculating aids kind and the impact of adding proportion on turbidity thereof.By test of many times, determine that adopting ground phosphate rock or calcium sulfate is flocculating aids, adding proportion is 0.1-2kg/m
3time, can obtain comparatively ideal effect.When above-mentioned mixed solution is carried out to one-level solid-liquid separation with plate-and-frame filter press, flocculating aids and flocs unit are all filtered, and can not enter in filtrate its quality is impacted, and after one-level solid-liquid separation, the turbidity of filtrate is 6-10.
The impact on turbidity of table 2 flocculating aids kind and adding proportion thereof
After above-mentioned coagulation, in filtrate, still there is a small amount of albumen and colloid, therefore need to carry out secondary precision processing, through test of many times checking in table 3, find that the result of inorganic flocculating agent is more satisfactory, so adopting polymerize aluminum chloride or polyaluminium sulfate in step (4) is flocculation agent, adding proportion is 0.1-1g/m
3, and because they are food grade, can not affect fermented liquid mass formation.
Table 3 flocculation agent kind and adding proportion thereof and colloid protein content relation
Equally, for ease of filtering, step still needs to add flocculating aids in (5), and in table 4, test of many times proves, diatomite and perlite are more satisfactory, and the ratio of interpolation is 0.1-2kg/m
3, wherein perlitic cost is lower, because it can not enter into filtrate, therefore can be not influential to fermented liquid quality.Flocculating aids forms coating on flocs unit surface, by its dispersion.Owing to filtering feed liquid, it is citric acid fermentation broth, to reduce as far as possible the residual acid in filter cake, therefore use the barrier film plate-and-frame filter press with pressing function to carry out secondary solid-liquid separation, obtain the clarified broth of turbidity < 5, and its level of automation is high, running cost is lower.
The impact on turbidity of table 4 flocculating aids kind and adding proportion thereof
In coagulation described above and second-stage treatment, after adding flocculation agent or flocculating aids, in order to mix, churning time is 20-30min.In filtration procedure, for the settling of floccus impact that prevents from generating, to filter and reduce power consumption, stirring velocity can be slightly slow, 20-30rpm.If churning time is too short, the effect of filtration is poor, and the long impact of churning time is little.
In actually operating, comprise at least two stages for the treatment of, coagulation is different from the flocculation agent adding in second-stage treatment and flocculating aids, and working order cannot be put upside down, to guarantee that flocculation and drainage can reach best effect.If after two stages for the treatment of, do not reach desired standard, can proceed multiple-stage treatment.
In sum, technical process of the present invention is succinct, can operate by continous-stable, the present invention does from feed liquid source, greatly alleviated the facility load of subsequent disposal, significantly reduced production run cost, the fermented liquid clarity of acquisition is good, the foreign matter content such as colloid, albumen is low, thereby guarantees to obtain better yield and quality product when secondary crystallization.
Accompanying drawing explanation
Fig. 1 is process flow diagram of the present invention.
Embodiment
Embodiment 1:
Filter a defecation method, comprise the following steps:
1, in citric acid fermentation broth, add flocculation agent polyacrylamide and stir, adding proportion is 0.1g/m
3;
2, in citric acid fermentation broth, add flocculating aids ground phosphate rock and stir, adding proportion is 1.0kg/m
3;
3, above-mentioned mixed solution is carried out to one-level solid-liquid separation with plate-and-frame filter press;
4, in the filtrate after filtering, again add flocculant poly aluminum chloride and stir, adding proportion is 0.3g/m
3;
5, in the filtrate after filtering, again add flocculating aids diatomite and stir, adding proportion is 0.5kg/m
3;
6, above-mentioned mixed solution is carried out to secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 3.27.
Embodiment 2:
Filter a defecation method, comprise the following steps:
1, in citric acid fermentation broth, add flocculation agent sodium polyacrylate and stir, adding proportion is 2.0g/m
3;
2, in citric acid fermentation broth, add flocculating aids calcium sulfate and stir, adding proportion is 0.5kg/m
3;
3, above-mentioned mixed solution is carried out to one-level solid-liquid separation with plate-and-frame filter press;
4, in the filtrate after filtering, again add flocculant poly Tai-Ace S 150 and stir, adding proportion is 0.1g/m
3;
5, in the filtrate after filtering, again add flocculating aids perlite and stir, adding proportion is 1.0kg/m
3;
6, above-mentioned mixed solution is carried out to secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 3.52.
Embodiment 3:
Filter a defecation method, comprise the following steps:
1, in citric acid fermentation broth, add flocculation agent polyacrylamide and stir, adding proportion is 1.0g/m
3;
2, in citric acid fermentation broth, add flocculating aids ground phosphate rock and stir, adding proportion is 0.1kg/m
3;
3, above-mentioned mixed solution is carried out to one-level solid-liquid separation with plate-and-frame filter press;
4, in the filtrate after filtering, again add flocculant poly aluminum chloride and stir, adding proportion is 0.7g/m
3;
5, in the filtrate after filtering, again add flocculating aids diatomite and stir, adding proportion is 2.0kg/m
3;
6, above-mentioned mixed solution is carried out to secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 4.45.
Embodiment 4:
Filter a defecation method, comprise the following steps:
1, in citric acid fermentation broth, add flocculation agent polyacrylamide and stir, adding proportion is 1.5g/m
3;
2, in citric acid fermentation broth, add flocculating aids calcium sulfate and stir, adding proportion is 2.0kg/m
3;
3, above-mentioned mixed solution is carried out to one-level solid-liquid separation with plate-and-frame filter press;
4, in the filtrate after filtering, again add flocculant poly aluminum chloride and stir, adding proportion is 1.0g/m
3;
5, in the filtrate after filtering, again add flocculating aids perlite and stir, adding proportion is 0.1kg/m
3;
6, above-mentioned mixed solution is carried out to secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 3.56.
Embodiment 5:
Filter a defecation method, comprise the following steps:
1, in citric acid fermentation broth, add flocculation agent sodium polyacrylate and stir, adding proportion is 0.5g/m
3;
2, in citric acid fermentation broth, add flocculating aids ground phosphate rock and stir, adding proportion is 1.5kg/m
3;
3, above-mentioned mixed solution is carried out to one-level solid-liquid separation with plate-and-frame filter press;
4, in the filtrate after filtering, again add flocculant poly Tai-Ace S 150 and stir, adding proportion is 0.5g/m
3;
5, in the filtrate after filtering, again add flocculating aids diatomite and stir, adding proportion is 1.5kg/m
3;
6, above-mentioned mixed solution is carried out to secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 2.98.
Above embodiment chooses in actual production process at random, and index contrast is only representational, specifically relates to many detection indexs, at this, does not elaborate
Comparative example
Get with isocyatic citric acid fermentation broth, adopt respectively traditional technology and technique of the present invention to process.
In original process, karusen carries out initial filter and multiple filter is processed, and table 5 is fermentation filtrate index contrast table, and wherein colourity is by absorbancy value, and this value is larger, and fermented liquid color is darker; Colloid protein content is the mass ratio that accounts for fermented liquid.From wherein finding out, adopt the present invention, its turbidity obviously reduces, and transparency is better, and colourity reduces, and colloid protein content is reduced to 0.01-0.03% by 0.1-0.2%.
Table 5 fermentation filtrate index contrast table
| Average turbidity | Colourity | Colloid protein content | |
| Original | 11.50 | 82-88％ | 0.1-0.2％ |
| The present invention | 3.48 | 70-75％ | 0.01-0.03％ |
Claims (2)
1. citric acid fermentation broth filters a defecation method, comprises at least two stages for the treatment of, it is characterized in that:
1) first coagulation adds flocculation agent to stir in citric acid fermentation broth, then adds flocculating aids to stir, and carries out solid-liquid separation;
2) second-stage treatment adds flocculation agent to stir in above-mentioned filtrate, then adds flocculating aids to stir, and carries out solid-liquid separation;
The flocculation agent adding in described coagulation is different from the flocculation agent adding in second-stage treatment and flocculating aids with flocculating aids;
Before described coagulation, added flocculation agent is polyacrylamide or sodium polyacrylate, and adding proportion is 0.1-2g/m
3;
Before described coagulation, added flocculating aids is ground phosphate rock or calcium sulfate, and adding proportion is 0.1-2kg/m
3;
Before described second-stage treatment, added flocculation agent is polymerize aluminum chloride or polyaluminium sulfate, and adding proportion is 0.1-lg/m
3;
Before described second-stage treatment, added flocculating aids is diatomite or perlite, and adding proportion is 0.1-2kg/m
3.
2. citric acid fermentation broth according to claim 1 filters defecation method, it is characterized in that: the solid-liquid separating equipment described in coagulation and second-stage treatment is plate-and-frame filter press.
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| CN102747110B (en) * | 2012-06-20 | 2014-01-08 | 日照鲁信金禾生化有限公司 | Treatment process for waste sodium citrate mother solution |
| CA2928805C (en) | 2013-11-19 | 2022-03-29 | Kemira Oyj | Method for reducing colour of a biotechnological liquid |
| CN105399617A (en) * | 2015-12-21 | 2016-03-16 | 日照金禾博源生化有限公司 | Method for filtering and clarifying citric acid fermentation broth |
| CN106241983A (en) * | 2016-08-11 | 2016-12-21 | 张慧坤 | A kind of purification process technique of waste or used plastics wash water |
| CN106365985B (en) * | 2016-08-31 | 2019-03-05 | 江苏国信协联能源有限公司 | A kind of depth filtration approaches of citric acid fermentation broth |
| CN114307389A (en) * | 2021-12-16 | 2022-04-12 | 潍坊英轩实业有限公司 | Filtering method for improving citric acid extraction yield and feed liquid quality |
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| CA940470A (en) * | 1969-07-29 | 1974-01-22 | Steven H. Corwin | Flocculation of fermentation broths |
| CN101182079B (en) * | 2007-11-26 | 2010-06-30 | 日照金禾生化集团有限公司 | Citric acid mother liquor treatment process |
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