CN102249894A - Method for filtering and clarifying citric acid fermentation broth - Google Patents
Method for filtering and clarifying citric acid fermentation broth Download PDFInfo
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- CN102249894A CN102249894A CN2010105836933A CN201010583693A CN102249894A CN 102249894 A CN102249894 A CN 102249894A CN 2010105836933 A CN2010105836933 A CN 2010105836933A CN 201010583693 A CN201010583693 A CN 201010583693A CN 102249894 A CN102249894 A CN 102249894A
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Abstract
The invention relates to a method for filtering and clarifying a citric acid fermentation broth. The method is characterized by the steps that: before the citric acid fermentation broth is processed, various flocculating agents and filter aids are added to feed liquid; filtering is then carried out, such that the clarified fermentation broth with a turbidity lower than 5 is obtained. With the method provided by the invention, quality of the fermentation broth is substantially improved. Not only the turbidity of the fermentation broth is substantially reduced, but the contents of internal colloids, proteins and other impurities in the fermentation broth are also reduced. Therefore, a burden on post extraction is greatly reduced.
Description
Technical field
The present invention relates to biological chemical field, be specifically related to a kind of citric acid fermentation broth and filter defecation method.
Background technology
Citric acid is first acid in the organic acid, because the performance of physicals, chemical property, derivative is widely used in industries such as food, medicine, daily use chemicals.In the suitability for industrialized production, citric acid mainly is to be that raw material makes through submerged fermentation with starchy materials such as corn, cassavas.Can obtain a large amount of citric acid fermentation wine with dregs after the fermentation ends, wherein contain many impurity,,, will be difficult to directly enter the back and extract workshop section as without purifying and filtration treatment as mycelium, albumen, colloid, pigment etc.At present, general way is that karusen is carried out filter just and multiple filter processing, and the clear liquid of acquisition is transported to the back again and extracts workshop section.The shortcoming of this technology was that cleaner liquid is of poor quality, unstable, its turbidity is usually more than 10, and the back being extracted materials such as the bigger albumen of influence, colloid is effectively removed, it is passive to cause the back to extract, every unit consumption index is obviously higher, the byproduct calcium sulfate color that obtains is relatively poor, is unfavorable for follow-up comprehensively utilization again.
Though in the patent No. is 200710113693.5 patent, disclose a kind of treatment process of citric acid mother liquor, impurity such as colloid wherein are effectively removed, obtained turbidity preferably, but had essence different, can't be applied to the processing of citric acid fermentation broth with the present invention.Compare with citric acid mother liquor, the citric acid fermentation broth composition is complicated more, amount is big, handle difficulty more, therefore the processing cost height, needs to seek a kind of effective filtering fermentation liquor defecation method, do from the feed liquid source, improving the shortcoming that the fermented liquid turbidity is poor, protein-colloid content is high, thereby greatly alleviate the facility load of subsequent disposal, significantly reduce the production run cost.
Summary of the invention
At above existing problems, the present invention proposes a kind of filtration defecation method of citric acid fermentation broth, adopt present method, can be effective improve the shortcoming that the fermented liquid turbidity is poor, protein-colloid content is high, obtain high-quality citric acid fermentation broth.
Key step is as follows:
1) one-level is handled
At first in citric acid fermentation broth, add flocculation agent and stir, add flocculating aids then and stir, carry out solid-liquid separation;
2) second-stage treatment
In above-mentioned filtrate, add flocculation agent and stir, add flocculating aids then and stir, carry out solid-liquid separation;
The flocculation agent and the flocculating aids that add in the flocculation agent that described one-level is added in handling and flocculating aids and the second-stage treatment are different, and its concrete processing step is as follows:
(1), in citric acid fermentation broth, add flocculation agent and stir, adding proportion is 0.1-2g/m
3
(2), add flocculating aids and stir, adding proportion is 0.1-2kg/m to citric acid fermentation broth
3
(3), above-mentioned mixed solution is carried out the one-level solid-liquid separation with plate-and-frame filter press;
(4), in filtrate filtered, add flocculation agent once more and stir, adding proportion is 0.1-1g/m
3
(5), in filtrate filtered, add flocculating aids once more and stir, adding proportion is 0.1-2kg/m
3
(6), above-mentioned mixed solution is carried out the secondary solid-liquid separation with plate-and-frame filter press.
In step (1), best by the flocculating effect of test of many times proof polyacrylamide or sodium polyacrylate, they can flocculate with soluble proteins, the colloid in the fermented liquid, thereby it is precipitated out.Table 1 is the influence to the colloid protein content of flocculation agent kind and adding proportion thereof.Draw by table 1, when the adding proportion of polyacrylamide/sodium polyacrylate is 0.1-2g/m
3The time, flocculating effect is better, not only albumen and colloid is fully flocculated but also can guarantee that flocculation agent can not remain in the fermented liquid, and the quality of citric acid fermentation broth is exerted an influence.
Table 1 flocculation agent kind and adding proportion thereof and colloid protein content relation
In step (2), adding flocculating aids is to help the filter coating for the flocs unit surface that produces in step (1) forms, and flocs unit is disperseed, and is easy to filter.Because this is filtered into one-level and filters, relatively low to the requirement of filtrate turbidity, so select for use the flocculating aids of relative low price to get final product.Table 2 is the influence to turbidity of flocculating aids kind and adding proportion thereof.Determine that by test of many times adopting ground phosphate rock or calcium sulfate is flocculating aids, adding proportion is 0.1-2kg/m
3The time, can obtain comparatively ideal effect.When above-mentioned mixed solution was carried out the one-level solid-liquid separation with plate-and-frame filter press, flocculating aids and flocs unit all were filtered, and can not enter in the filtrate its quality is impacted, and the turbidity of one-level solid-liquid separation rear filtrate is 6-10.
Table 2 flocculating aids kind and adding proportion thereof are to the influence of turbidity
After above-mentioned one-level processing, still there are a spot of albumen and colloid in the filtrate, therefore need carry out the secondary precision processing, find that through test of many times checking in the table 3 result of inorganic flocculating agent is more satisfactory, so adopting polymerize aluminum chloride or polyaluminium sulfate in the step (4) is flocculation agent, adding proportion is 0.1-1g/m
3, and because they are food grade, can not exert an influence to the fermented liquid quality.
Table 3 flocculation agent kind and adding proportion thereof and colloid protein content relation
Equally, in order to be easy to filter, step still needs to add flocculating aids in (5), and test of many times proves in table 4, and diatomite and perlite are more satisfactory, and the ratio of interpolation is 0.1-2kg/m
3, wherein perlitic cost is lower, because it can not enter into filtrate, so can be not influential to the fermented liquid quality.Flocculating aids forms coating on the flocs unit surface, with its dispersion.Because filtering feed liquid is citric acid fermentation broth, to reduce the residual acid in the filter cake as far as possible, therefore use barrier film plate-and-frame filter press to carry out the secondary solid-liquid separation, obtain the clarified broth of turbidity＜5 with pressing function, and its level of automation height, running cost is lower.
Table 4 flocculating aids kind and adding proportion thereof are to the influence of turbidity
In above-mentioned described one-level processing and second-stage treatment, add after flocculation agent or the flocculating aids, in order to mix, churning time is 20-30min.In filtration procedure, to filter and the reduction power consumption for the settling of floccus influence that prevents to generate, stirring velocity can be slow slightly, and 20-30rpm gets final product.If churning time is too short, filtering effect is relatively poor, and the long then influence of churning time is little.
In actually operating, comprise bi-level treatment at least, one-level handle with second-stage treatment in the flocculation agent and the flocculating aids that add be different, working order cannot be put upside down, and flocculates and helps filter can both reach best effect guaranteeing.If through after the bi-level treatment, do not reach desired standard, can proceed multiple-stage treatment.
In sum, technical process of the present invention is succinct, but continous-stable operation, the present invention does from the feed liquid source, greatly alleviated the facility load of subsequent disposal, significantly reduced the production run cost, the fermented liquid clarity of acquisition is good, foreign matter content such as colloid, albumen is low, thereby guarantees to obtain when secondary crystallization better yield and quality product.
Description of drawings
Fig. 1 is a process flow diagram of the present invention.
Embodiment
Embodiment 1:
A kind of citric acid fermentation broth filters defecation method, may further comprise the steps:
1, add flocculation agent polyacrylamide and stirring in citric acid fermentation broth, adding proportion is 0.1g/m
3
2, add flocculating aids ground phosphate rock and stirring in citric acid fermentation broth, adding proportion is 1.0kg/m
3
3, above-mentioned mixed solution is carried out the one-level solid-liquid separation with plate-and-frame filter press;
4, add flocculant poly aluminum chloride and stirring once more in filtrate filtered, adding proportion is 0.3g/m
3
5, add flocculating aids diatomite and stirring once more in filtrate filtered, adding proportion is 0.5kg/m
3
6, above-mentioned mixed solution is carried out the secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 3.27.
Embodiment 2:
A kind of citric acid fermentation broth filters defecation method, may further comprise the steps:
1, add flocculation agent sodium polyacrylate and stirring in citric acid fermentation broth, adding proportion is 2.0g/m
3
2, add flocculating aids calcium sulfate and stirring in citric acid fermentation broth, adding proportion is 0.5kg/m
3
3, above-mentioned mixed solution is carried out the one-level solid-liquid separation with plate-and-frame filter press;
4, add flocculant poly Tai-Ace S 150 and stirring once more in filtrate filtered, adding proportion is 0.1g/m
3
5, add flocculating aids perlite and stirring once more in filtrate filtered, adding proportion is 1.0kg/m
3
6, above-mentioned mixed solution is carried out the secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 3.52.
Embodiment 3:
A kind of citric acid fermentation broth filters defecation method, may further comprise the steps:
1, add flocculation agent polyacrylamide and stirring in citric acid fermentation broth, adding proportion is 1.0g/m
3
2, add flocculating aids ground phosphate rock and stirring in citric acid fermentation broth, adding proportion is 0.1kg/m
3
3, above-mentioned mixed solution is carried out the one-level solid-liquid separation with plate-and-frame filter press;
4, add flocculant poly aluminum chloride and stirring once more in filtrate filtered, adding proportion is 0.7g/m
3
5, add flocculating aids diatomite and stirring once more in filtrate filtered, adding proportion is 2.0kg/m
3
6, above-mentioned mixed solution is carried out the secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 4.45.
Embodiment 4:
A kind of citric acid fermentation broth filters defecation method, may further comprise the steps:
1, add flocculation agent polyacrylamide and stirring in citric acid fermentation broth, adding proportion is 1.5g/m
3
2, add flocculating aids calcium sulfate and stirring in citric acid fermentation broth, adding proportion is 2.0kg/m
3
3, above-mentioned mixed solution is carried out the one-level solid-liquid separation with plate-and-frame filter press;
4, add flocculant poly aluminum chloride and stirring once more in filtrate filtered, adding proportion is 1.0g/m
3
5, add flocculating aids perlite and stirring once more in filtrate filtered, adding proportion is 0.1kg/m
3
6, above-mentioned mixed solution is carried out the secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 3.56.
Embodiment 5:
A kind of citric acid fermentation broth filters defecation method, may further comprise the steps:
1, add flocculation agent sodium polyacrylate and stirring in citric acid fermentation broth, adding proportion is 0.5g/m
3
2, add flocculating aids ground phosphate rock and stirring in citric acid fermentation broth, adding proportion is 1.5kg/m
3
3, above-mentioned mixed solution is carried out the one-level solid-liquid separation with plate-and-frame filter press;
4, add flocculant poly Tai-Ace S 150 and stirring once more in filtrate filtered, adding proportion is 0.5g/m
3
5, add flocculating aids diatomite and stirring once more in filtrate filtered, adding proportion is 1.5kg/m
3
6, above-mentioned mixed solution is carried out the secondary solid-liquid separation with plate-and-frame filter press, the ferment filtrate turbidity of acquisition is 2.98.
Above embodiment is a picked at random in the actual production process, and the index contrast only is representational, specifically relates to many detection indexs, does not elaborate at this
Comparative example
Get citric acid fermentation broth, adopt traditional technology and technology of the present invention to handle respectively with isoconcentration.
In the original process, karusen carries out filter just and multiple filter is handled, and table 5 is fermentation filtered liquid index contrast table, and wherein colourity is by the absorbancy value, and this value is big more, and the fermented liquid color is dark more; The colloid protein content is the mass ratio that accounts for fermented liquid.From wherein as can be seen, adopt the present invention, its turbidity obviously reduces, and transparency is better, and colourity reduces, and the colloid protein content is reduced to 0.01-0.03% by 0.1-0.2%.
Table 5 fermentation filtered liquid index contrast table
| Average turbidity | Colourity | The colloid protein content | |
| Original | 11.50 | 82-88％ | 0.1-0.2％ |
| The present invention | 3.48 | 70-75％ | 0.01-0.03％ |
Claims (6)
1. a citric acid fermentation broth filters defecation method, comprises bi-level treatment at least, it is characterized in that:
1) one-level is handled
At first in citric acid fermentation broth, add flocculation agent and stir, add flocculating aids then and stir, carry out solid-liquid separation;
2) second-stage treatment
In above-mentioned filtrate, add flocculation agent and stir, add flocculating aids then and stir, carry out solid-liquid separation;
The flocculation agent and the flocculating aids that add in the flocculation agent that described one-level is added in handling and flocculating aids and the second-stage treatment are different.
2. treatment process according to claim 1 is characterized in that: added flocculation agent was polyacrylamide or sodium polyacrylate before one-level was handled, and adding proportion is 0.1-2g/m
3
3. treatment process according to claim 1 is characterized in that: added flocculating aids was ground phosphate rock or calcium sulfate before one-level was handled, and adding proportion is 0.1-2kg/m
3
4. treatment process according to claim 1 is characterized in that: added flocculation agent is polymerize aluminum chloride or polyaluminium sulfate before the second-stage treatment, and adding proportion is 0.1-1g/m
3
5. treatment process according to claim 1 is characterized in that: added flocculating aids is diatomite or perlite before the second-stage treatment, and adding proportion is 0.1-2kg/m
3
6. treatment process according to claim 1 is characterized in that: the solid-liquid separating equipment described in one-level processing and the second-stage treatment is a plate-and-frame filter press.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747110A (en) * | 2012-06-20 | 2012-10-24 | 日照鲁信金禾生化有限公司 | Treatment process for waste sodium citrate mother solution |
| CN105399617A (en) * | 2015-12-21 | 2016-03-16 | 日照金禾博源生化有限公司 | Method for filtering and clarifying citric acid fermentation broth |
| CN106241983A (en) * | 2016-08-11 | 2016-12-21 | 张慧坤 | A kind of purification process technique of waste or used plastics wash water |
| CN106365985A (en) * | 2016-08-31 | 2017-02-01 | 江苏国信协联能源有限公司 | Deep filtering method for citric acid fermentation liquid |
| US10604546B2 (en) | 2013-11-19 | 2020-03-31 | Kemira Oyj | Method for reducing colour of a biotechnological liquid |
| CN114307389A (en) * | 2021-12-16 | 2022-04-12 | 潍坊英轩实业有限公司 | Filtering method for improving citric acid extraction yield and feed liquid quality |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1323902A (en) * | 1969-07-29 | 1973-07-18 | Drew Chem Corp | Clarification of fermentation broths |
| CN101182079A (en) * | 2007-11-26 | 2008-05-21 | 日照金禾生化集团有限公司 | Citric acid mother liquor treatment process |
-
2010
- 2010-12-13 CN CN201010583693.3A patent/CN102249894B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1323902A (en) * | 1969-07-29 | 1973-07-18 | Drew Chem Corp | Clarification of fermentation broths |
| CN101182079A (en) * | 2007-11-26 | 2008-05-21 | 日照金禾生化集团有限公司 | Citric acid mother liquor treatment process |
Non-Patent Citations (1)
| Title |
|---|
| 欧阳平凯: "《生物分离原理及技术》", 28 February 1999, 化学工业出版社 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747110A (en) * | 2012-06-20 | 2012-10-24 | 日照鲁信金禾生化有限公司 | Treatment process for waste sodium citrate mother solution |
| CN102747110B (en) * | 2012-06-20 | 2014-01-08 | 日照鲁信金禾生化有限公司 | Treatment process for waste sodium citrate mother solution |
| US10604546B2 (en) | 2013-11-19 | 2020-03-31 | Kemira Oyj | Method for reducing colour of a biotechnological liquid |
| CN105399617A (en) * | 2015-12-21 | 2016-03-16 | 日照金禾博源生化有限公司 | Method for filtering and clarifying citric acid fermentation broth |
| CN106241983A (en) * | 2016-08-11 | 2016-12-21 | 张慧坤 | A kind of purification process technique of waste or used plastics wash water |
| CN106365985A (en) * | 2016-08-31 | 2017-02-01 | 江苏国信协联能源有限公司 | Deep filtering method for citric acid fermentation liquid |
| CN106365985B (en) * | 2016-08-31 | 2019-03-05 | 江苏国信协联能源有限公司 | A kind of depth filtration approaches of citric acid fermentation broth |
| CN114307389A (en) * | 2021-12-16 | 2022-04-12 | 潍坊英轩实业有限公司 | Filtering method for improving citric acid extraction yield and feed liquid quality |
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| CN102249894B (en) | 2014-02-05 |
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