CN102246746A - Fish sperm preserving fluid and preserving method - Google Patents
Fish sperm preserving fluid and preserving method Download PDFInfo
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- CN102246746A CN102246746A CN2011101538361A CN201110153836A CN102246746A CN 102246746 A CN102246746 A CN 102246746A CN 2011101538361 A CN2011101538361 A CN 2011101538361A CN 201110153836 A CN201110153836 A CN 201110153836A CN 102246746 A CN102246746 A CN 102246746A
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- sperm
- fish sperm
- fish
- seminal fluid
- preserving
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Abstract
The invention discloses a fish sperm preserving fluid which is prepared from 2.120-2.420g of NaCl, 1.602-2.202g of KCL, 0.178-0.478g of CaCl2, 0.133-0.333g of MgSO4.7H2O, 0.114-0.414g of NaHCO3, 0.128-0.328g of VC and 500mL of distilled water. The fish sperm preserving method comprises the following steps of: (a) sperm collection: wiping and drying the genital pore region of a mature milter by using a towel, slightly pressing the abdomen of the mature milter, and collecting the extruded sperm by using a suction tube; and (b) sperm preservation: putting the collected sperm into a clean container, adding the sperm preserving fluid of which the volume is 10-20 times of the volume of the sperm, and preserving in low-temperature light-tight places.
Description
Technical field
The present invention relates to a kind of fish sperm and preserve liquid and store method, belong to the animal reproduction technical field.
Background technology
China is the country of a water biological species resource than horn of plenty, and rich and varied water biological species resource has played important effect to the development of China's culture fishery.Minnow subfamily fish are a kind of small fishes that move in lower floor in the water body, and most of river and attached water body thereof in China flat area all have distribution.Though the artificial propagation techniques of a lot of fish is broken through, Shi Minnow subfamily artificial propagation of fish is not also realized.Because the long-term overfishing of fishery resources and the pollution ， Minnow subfamily stock of fish slump of disastrous proportions of water environment and endangered.A lot of fish are owing to solved an artificial propagation techniques difficult problem, and species resource is effectively protected and makes full use of.Minnow subfamily fish are owing to reasons such as seminal fluid rarenesses, and artificial propagation techniques does not solve so far.The artificial propagation of Kai Zhan Minnow subfamily fish, and species resource developed, seem particularly important.
Summary of the invention
For understanding the problem that the Minnow subfamily stock of fish amount of determining decays day by day, root of the present invention has designed sperm preservation liquid and store method according to the reproductive biology characteristics of Minnow subfamily fish, and this technology is preserved by the dilution of sperm, the real artificial propagation of Minnow subfamily fish now.
In order to reach above purpose, the technical solution used in the present invention is: a kind of fish sperm is preserved liquid, and every 100ml should preserve and comprise following composition in the liquid:
NaCl 0.424g～0.484g
KCl 0.320～0.440g
CaCl
2
0.036 g～0.096g
MgSO
4
·7H
2
O 0.027g～0.067g
NaHCO
3
0.023～0.083g
V
C
0.026g～0.066g。
Preferred fish sperm is preserved liquid, and every 100mL should preserve and comprise following composition in the liquid:
NaCl 0.464g
KCl 0.380g
CaCl
2
0.066g
MgSO
4
·7H
2
O 0.047g
NaHCO
3
0.053g
V
C
0.046g。
Another program of the present invention provides a kind of fish sperm store method, the steps include:
(a) semen collection: towel off and do ripe milter gonopore zone, gently press belly, collect the seminal fluid of extruding with suction pipe;
(b) seminal fluid is deposited: the seminal fluid that collects is placed clean container, add the fish sperm preservation liquid with respect to 10～20 times of volumes of seminal fluid volume, deposit in the low-temperature dark place.
Preferably, the container in the step (b) has lid, and it is airtight that lid is built the back container.
More preferably, the seminal fluid storage temperature is 3～5 ℃ in the step (b).
The present invention is by adopting above technical scheme; can effectively prolong the external time-to-live of fish sperm; the integrality of protection sperm; significantly improved the survival index of fish sperm; keep its fertilization vigor; Shi Xian Minnow subfamily fish parent's artificial induced spawning, and the artificial propagation of the problem Shi Xian Minnow subfamily fish that Xie Jue Minnow subfamily fish sperm is few, fertilization rate is low.Fish sperm of the present invention is preserved liquid and store method both had been applicable to that the artificial insemination bred, and is applicable to that also the short-term of fish sperm is preserved.Simultaneously sperm preserve the preparation of liquid simple, draw materials easily, the sperm store method is convenient to operation, a whole set of technical scheme is with low cost, and is easy to promote.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby protection scope of the present invention is made more explicit defining so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that.
For improving the survival rate of fish sperm, the artificial propagation of Shi Xian Minnow subfamily fish, the employing of fish sperm preservation liquid NaCl, KCl, CaCl
2
, MgSO
4
7H
2
O, NaHCO
3
, V
C
Etc. various compositions, formulated with distilled water.
Fish sperm in the present embodiment is preserved liquid, comprises following composition:
NaCl 2.320g
KCl 1.902g
CaCl
2
0.328g
MgSO
4
·7H
2
O 0.233g
NaHCO
3
0.264g
V
C
0.228g
Distilled water 500mL.
Its compound method is as follows:
At first take by weighing 2.320g NaCl, be dissolved in the 10ml distilled water, it is dissolved fully.Take by weighing 1.902g KCl and be dissolved in the 50ml distilled water, add 0.328g CaCl respectively after treating to dissolve fully
2
, 0.233g MgSO
4
7H
2
O, 0.264g NaHCO
3
, add one by one according to above order, a kind of under treating during interpolation to add again after the reagent that adds earlier dissolves fully, after the dissolving two kinds of preparations are mixed mutually, add 0.228g V then
C
, add distilled water 500ml at last.
Utilize above-mentioned fish sperm to preserve a kind of fish sperm store method of liquid, the steps include:
(a) semen collection towels off and does ripe milter gonopore zone, gently presses belly, collects the seminal fluid of extruding with suction pipe;
(b) seminal fluid is deposited, and the seminal fluid that collects is placed container clean, that have lid, adds the fish sperm preservation liquid with respect to 10～20 times of volumes of seminal fluid volume, has changed lid, makes container airtight, and airtight container is deposited in the low-temperature dark place.
In the present embodiment airtight container is deposited in 4 ℃ the refrigerator.
Above embodiment only is explanation technical conceive of the present invention and characteristics; its purpose is to allow the people that is familiar with this technology understand content of the present invention and is implemented; can not limit protection scope of the present invention with this; all equivalences that spirit essence is done according to the present invention change or modify, and all should be encompassed in protection scope of the present invention.
Claims (5)
1. a fish sperm is preserved liquid, and it is characterized in that: every 100mL should preserve liquid and comprise NaCl 0.424g～0.484g, KCl 0.320～0.440g, CaCl
20.036 g～0.096g, MgSO
47H
2O 0.027g～0.067g, NaHCO
30.023～0.083g, V
C0.026g～0.066g.
2. fish sperm according to claim 1 is preserved liquid, and it is characterized in that: every 100mL should preserve liquid and comprise NaCl 0.464g, KCl 0.380g, CaCl
20.066g, MgSO
47H
2O 0.047g, NaHCO
30.053g, V
C0.046g.
3. a fish sperm store method that utilizes the described fish sperm of claim 1 to preserve liquid the steps include:
(a) semen collection: towel off and do ripe milter gonopore zone, gently press belly, collect the seminal fluid of extruding with suction pipe;
(b) seminal fluid is deposited: the seminal fluid that collects is placed clean container, add the described fish sperm preservation liquid with respect to 10～20 times of volumes of seminal fluid volume, deposit in the low-temperature dark place.
4. fish sperm store method according to claim 3 is characterized in that: the container described in the step (b) has lid, and it is airtight that lid is built the back container.
5. fish sperm store method according to claim 3 is characterized in that: the seminal fluid storage temperature is 3～5 ℃ described in the step (b).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101538361A CN102246746A (en) | 2011-06-09 | 2011-06-09 | Fish sperm preserving fluid and preserving method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101538361A CN102246746A (en) | 2011-06-09 | 2011-06-09 | Fish sperm preserving fluid and preserving method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102246746A true CN102246746A (en) | 2011-11-23 |
Family
ID=44974612
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101538361A Pending CN102246746A (en) | 2011-06-09 | 2011-06-09 | Fish sperm preserving fluid and preserving method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102246746A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986650A (en) * | 2012-12-19 | 2013-03-27 | 中国水产科学研究院长江水产研究所 | Finless eel sperm diluent and preparation method thereof |
| CN103931606A (en) * | 2014-04-04 | 2014-07-23 | 湖南苗王生物科技有限公司 | Preparation method for vitrification freezing-thawing protection liquid of sturgeon sperms |
| CN104814005A (en) * | 2015-03-26 | 2015-08-05 | 谢光玉 | Preservation method for seminal fluid of Schizothorax davidi |
| CN105230609A (en) * | 2015-11-06 | 2016-01-13 | 刘玉鹏 | Formula of basic protection liquid for fish sperms |
| CN105557683A (en) * | 2016-01-15 | 2016-05-11 | 清远职业技术学院 | Loach sperm preservation method and corresponding loach sperm preservation solution |
| CN109122668A (en) * | 2018-10-25 | 2019-01-04 | 镇江市绿色农业科技有限公司 | A kind of store method of hilsa herring sperm |
-
2011
- 2011-06-09 CN CN2011101538361A patent/CN102246746A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 宣云峰等: "华鳈人工繁殖试验", 《水产养殖》 * |
| 赵钦等: "黄颡鱼精子低温保存方法的初步研究及应用", 《水产科学》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986650A (en) * | 2012-12-19 | 2013-03-27 | 中国水产科学研究院长江水产研究所 | Finless eel sperm diluent and preparation method thereof |
| CN102986650B (en) * | 2012-12-19 | 2014-04-02 | 中国水产科学研究院长江水产研究所 | Finless eel sperm diluent and preparation method thereof |
| CN103931606A (en) * | 2014-04-04 | 2014-07-23 | 湖南苗王生物科技有限公司 | Preparation method for vitrification freezing-thawing protection liquid of sturgeon sperms |
| CN104814005A (en) * | 2015-03-26 | 2015-08-05 | 谢光玉 | Preservation method for seminal fluid of Schizothorax davidi |
| CN105230609A (en) * | 2015-11-06 | 2016-01-13 | 刘玉鹏 | Formula of basic protection liquid for fish sperms |
| CN105557683A (en) * | 2016-01-15 | 2016-05-11 | 清远职业技术学院 | Loach sperm preservation method and corresponding loach sperm preservation solution |
| CN109122668A (en) * | 2018-10-25 | 2019-01-04 | 镇江市绿色农业科技有限公司 | A kind of store method of hilsa herring sperm |
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Application publication date: 20111123 |