CN105230609A - Formula of basic protection liquid for fish sperms - Google Patents

Formula of basic protection liquid for fish sperms Download PDF

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Publication number
CN105230609A
CN105230609A CN201510750728.0A CN201510750728A CN105230609A CN 105230609 A CN105230609 A CN 105230609A CN 201510750728 A CN201510750728 A CN 201510750728A CN 105230609 A CN105230609 A CN 105230609A
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CN
China
Prior art keywords
formula
protection liquid
foundation
fish
basic protection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510750728.0A
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Chinese (zh)
Inventor
刘玉鹏
李敬荣
金艳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Liu Chengzhang
Liu Diming
Liu Yupeng
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201510750728.0A priority Critical patent/CN105230609A/en
Publication of CN105230609A publication Critical patent/CN105230609A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a formula of basic protection liquid for fish sperms. The specific scheme is as follows: 1.6-2 g of C6H1206, 4.5-4.8 g of NaC1, 3.6-3.8 g of KCl, 0.4-0.6 g of CaCl2, 0.3-0.6 g of MgC12.6H2O, 0.3-0.6 g of NaHCO3, 1.6-2 g of Na2HPO4, 0.5-0.9 g of KH2PO4, and 1L of distilled water. The formula is scientific in compatibility of medicines, the pH value is stable, the components are mostly inorganic matter, and are low in cost, easily obtained and environmentally friendly, a good environment can be provided for the survival of fish sperms, and a remarkable storage effect is achieved. The basic protection liquid for fish sperms, prepared according to the formula, has no harsh requirements for temperature, and can be stored and used in room temperature without being refrigerated.

Description

The formula of the sub-foundation to protect liquid of a kind of milt
Technical field
The present invention relates to a kind of formula protecting liquid, the formula of the sub-foundation to protect liquid of especially a kind of milt.
Background technology
China Patent Publication No. CN104957130A, publication date on October 7th, 2015, the name of innovation and creation is called a kind of method of goldfish ovum Cord blood, that application discloses a kind of foundation to protect liquid for preserving goldfish ovum, formulated by sodium chloride, ethylene glycol, glycerine, dimethyl sulfoxide (DMSO), shitosan and water, its weak point has used multiple organic chemical composition, contaminated environment and with high costs; Research for the foundation to protect liquid of fish sperm rarely has report.
Summary of the invention
For solving an above existing difficult problem, the invention discloses the formula of the sub-foundation to protect liquid of a kind of milt, concrete scheme is as follows:
C 6H 12O 61.6～2g
NaCl4.5～4.8g
KCl3.6～3.8g
CaCl 20.4～0.6g
MgCl 2·6H 2O0.3～0.6g
NaHCO 30.3～0.6g
Na 2HPO 41.6～2g
KH 2PO 40.5～0.9g
Distilled water 1L
The effect of each component:
C 6h 12o 6: to the activity makeup energy of sperm;
NaCl: the homeostasis keeping osmotic pressure inside and outside sperm;
KCl: the homeostasis keeping osmotic pressure inside and outside sperm;
CaCl 2: calcium ion can the activity of Inhibit sperm, improves the time-to-live;
MgCl 26H 2o: magnesium ion can the activity of Inhibit sperm, improves the time-to-live;
NaHCO 3: keep stable pH value better to preserve sperm as buffer;
Na 2hPO 4: keep stable pH value better to preserve sperm as buffer;
KH 2pO 4: keep stable pH value better to preserve sperm as buffer.
Beneficial effect: compatibility science of the present invention, pH value is stablized, and component mostly is inorganic substances, cheap and easy to get, environmental protection, can provide good environment, have significant preservation effect for the survival of fish sperm.
Embodiment
In conjunction with the embodiments, the present invention is described in further details.
Embodiment 1
A formula for the sub-foundation to protect liquid of milt, concrete scheme is as follows:
C 6H 12O 61.6g
NaCl4.5g
KCl3.6g
CaCl 20.4g
MgCl 2·6H 2O0.3g
NaHCO 30.3g
Na 2HPO 41.6g
KH 2PO 40.5g
Distilled water 1L
Embodiment 2
A formula for the sub-foundation to protect liquid of milt, concrete scheme is as follows:
C 6H 12O 62g
NaCl4.8g
KCl3.8g
CaCl 20.6g
MgCl 2·6H 2O0.6g
NaHCO 30.6g
Na 2HPO 42g
KH 2PO 40.9g
Distilled water 1L
Embodiment 3
A formula for the sub-foundation to protect liquid of milt, concrete scheme is as follows:
C 6H 12O 61.8g
NaCl4.68g
KCl3.725g
CaCl 20.555g
MgCl 2·6H 2O0.406g
NaHCO 34.2g
Na 2HPO 41.86g
KH 2PO 40.72g
Distilled water 1L
The milt foundation to protect liquid that formula of the present invention is made in use can be used in combination with the composition being applicable to certain specific fish sperm protection, also can be used alone or use through dilution.The milt foundation to protect liquid that formula of the present invention is made is not harsh to temperature requirement, and room temperature can preserve and use, without the need to refrigeration.
Above-mentioned embodiment is only for example of the present invention is clearly described, and the restriction not to embodiment.For those of ordinary skill in the field; can also make other changes in different forms on the basis of the above description; here exhaustive without the need to also giving all embodiments, and thus the apparent change of amplifying out or variation be still in protection scope of the present invention.

Claims (5)

1. a formula for the sub-foundation to protect liquid of milt, it is characterized in that, concrete scheme is as follows:
2. the formula of the sub-foundation to protect liquid of a kind of milt according to claim 1, it is characterized in that, concrete scheme is as follows:
3. the formula of the sub-foundation to protect liquid of a kind of milt according to claim 1, it is characterized in that, concrete scheme is as follows:
4. the formula of the sub-foundation to protect liquid of a kind of milt according to claim 1, is characterized in that, described C 6h 12o 6for glucose or sucrose.
5. the formula of the sub-foundation to protect liquid of a kind of milt according to claim 1, is characterized in that, described distilled water is redistilled water.
CN201510750728.0A 2015-11-06 2015-11-06 Formula of basic protection liquid for fish sperms Pending CN105230609A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510750728.0A CN105230609A (en) 2015-11-06 2015-11-06 Formula of basic protection liquid for fish sperms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510750728.0A CN105230609A (en) 2015-11-06 2015-11-06 Formula of basic protection liquid for fish sperms

Publications (1)

Publication Number Publication Date
CN105230609A true CN105230609A (en) 2016-01-13

Family

ID=55028679

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510750728.0A Pending CN105230609A (en) 2015-11-06 2015-11-06 Formula of basic protection liquid for fish sperms

Country Status (1)

Country Link
CN (1) CN105230609A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107624754A (en) * 2017-10-24 2018-01-26 浙江省淡水水产研究所 A kind of anti frozen liquid and its application method for Odontobulis mpotamophila sperm storage

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101502256A (en) * 2009-03-11 2009-08-12 宁波大学 Ultra low temperature cryopreservation method for sperm of large yellow crocker and cryopreservation device
US20100191041A1 (en) * 2009-01-24 2010-07-29 Xy, Inc. Reproductive technology of low dose semen production and in vitro / in vitro fertilization in domestic animals
CN101884322A (en) * 2010-07-20 2010-11-17 中国水产科学研究院黄海水产研究所 Verasper moseri sperm cryopreservation method
CN102246746A (en) * 2011-06-09 2011-11-23 吴江市水产养殖有限公司 Fish sperm preserving fluid and preserving method
CN102870763A (en) * 2011-07-11 2013-01-16 广东海洋大学 Cryopreservation method of Lutjanus erythropterus sperms
CN103563886A (en) * 2013-10-14 2014-02-12 苏州依科曼生物农业科技有限公司 Super-freezing storage method of yellow perch sperm

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100191041A1 (en) * 2009-01-24 2010-07-29 Xy, Inc. Reproductive technology of low dose semen production and in vitro / in vitro fertilization in domestic animals
CN101502256A (en) * 2009-03-11 2009-08-12 宁波大学 Ultra low temperature cryopreservation method for sperm of large yellow crocker and cryopreservation device
CN101884322A (en) * 2010-07-20 2010-11-17 中国水产科学研究院黄海水产研究所 Verasper moseri sperm cryopreservation method
CN102246746A (en) * 2011-06-09 2011-11-23 吴江市水产养殖有限公司 Fish sperm preserving fluid and preserving method
CN102870763A (en) * 2011-07-11 2013-01-16 广东海洋大学 Cryopreservation method of Lutjanus erythropterus sperms
CN103563886A (en) * 2013-10-14 2014-02-12 苏州依科曼生物农业科技有限公司 Super-freezing storage method of yellow perch sperm

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107624754A (en) * 2017-10-24 2018-01-26 浙江省淡水水产研究所 A kind of anti frozen liquid and its application method for Odontobulis mpotamophila sperm storage

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SE01 Entry into force of request for substantive examination
C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20160512

Address after: 221300 Sanxing garden, Zhujiang East Road, Yunhe street, Xuzhou City, Jiangsu Province, 25, 105, Pizhou

Applicant after: Liu Yupeng

Applicant after: Liu Chengzhang

Applicant after: Liu Diming

Address before: 221300, building 3, building 25, Sanxing garden, Zhujiang East Road, Yunhe street, Jiangsu, Pizhou, 105

Applicant before: Liu Yupeng

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20160113