CN102241653B - Membrane filtration and concentration technology of gibberellin - Google Patents
Membrane filtration and concentration technology of gibberellin Download PDFInfo
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- CN102241653B CN102241653B CN 201110190855 CN201110190855A CN102241653B CN 102241653 B CN102241653 B CN 102241653B CN 201110190855 CN201110190855 CN 201110190855 CN 201110190855 A CN201110190855 A CN 201110190855A CN 102241653 B CN102241653 B CN 102241653B
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Abstract
The invention provides a membrane filtration and concentration technology of gibberellin and belongs to an extraction and production method of a plant growth regulator. The technology comprises the following steps: filtering gibberellin fermentation liquor by use of a plate ultrafiltration membrane, spiral ultrafiltration membrane or tubular ultrafiltration membrane; and then concentrating by useof a tubular nanofiltration membrane or reverse osmosis membrance. By adopting the technology, the water consumption, power consumption and steam consumption can be greatly reduced and the concentration yield can be increased; and the technology can be implemented at the room temperature and the content of the main active ingredients of gibberellin can be increased in all.
Description
Technical field
The invention belongs to the extraction preparation method of a plant growth regulators, be specifically related to a kind of filtration, concentration technology of Plant hormones regulators,gibberellins.
Background technology
Plant hormones regulators,gibberellins is a class plant hormone that extensively exists, and generally synthetic in young shoot, young root and immature seed, its chemical structure belongs to diterpenes acid, is derived and is got by the Fourth Ring skeleton.The main effect of Plant hormones regulators,gibberellins is to promote cell elongation, thereby causes that cane elongation and plant increase.The Plant hormones regulators,gibberellins that uses in the production utilizes gibberella to carry out liquid fermenting and produces, and its filtration, concentration technology generally adopt the film under vacuum concentration technology, and the consumption of this method water power vapour is big, production cycle is long, cause Plant hormones regulators,gibberellins product heat-sensitive substance effective ingredient inactivation, product quality is poor, and yield is low.
Summary of the invention
The objective of the invention is at top described defective, provide a kind of and can reduce water power vapour energy consumption, shortening production cycle, reduce heat-sensitive substance effective ingredient inactivation, improve Plant hormones regulators,gibberellins membrane filtration, the concentration technology of extract yield and product quality.
The objective of the invention is to be achieved by the following technical programs.
A kind of membrane filtration of Plant hormones regulators,gibberellins, concentration technology is characterized in that may further comprise the steps successively:
Earlier with gibberellin fermented liquid through Plate Filtration, obtain sheet frame filtrate and sheet frame washing lotion, sheet frame filtrate and sheet frame washing lotion get ultrafiltrated and ultrafiltration and concentration liquid after flat plate ultrafiltration membrane filters again, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, and gets ultrafiltrated;
2. ultrafiltrated is adopted the rolling nanofiltration membrane to carry out one-level and concentrate 3-5v/v, adopt the tubular type nanofiltration membrane to carry out secondary and concentrate 1.5-3v/v, get concentrated solution, operating temperature is normal temperature.
The filtrate of sheet frame described in the step 1 and sheet frame washing lotion also available rolling ultra-filtration membrane or tubular ultra-filtration membrane are filtered, and mould material is a kind of in polysulfone membrane, polyethylene film, poly (ether sulfone) film, cellulose acetate film, polyvinylidene fluoride film, polyamide membrane or the composite membrane.
The specification of flat plate ultrafiltration membrane described in the step 1 is 1-10 ten thousand molecular weight cut-offs.
The specification of the ultra-filtration membrane of rolling described in the step 1 and tubular ultra-filtration membrane is 1-10 ten thousand molecular weight cut-offs.
Secondary described in the step 2 concentrates can adopt reverse osmosis membrane.
The specification of the nanofiltration membrane of rolling described in the step 2 and tubular type nanofiltration membrane is the 100-300 molecular weight cut-off, and mould material is a kind of in polysulfone membrane, polyethylene film, poly (ether sulfone) film, cellulose acetate film, polyvinylidene fluoride film, polyamide membrane or the composite membrane.
The specification of reverse osmosis membrane described in the step 2 is the 50-100 molecular weight cut-off.
The invention has the beneficial effects as follows: the present invention adopts flat plate ultrafiltration membrane or rolling ultra-filtration membrane to carry out extraction separation, and gibberellin fermented liquid foreign protein obtains more than 99% removing, and has improved filtrate quality; The present invention adopts rolling nanofiltration membrane, tubular type nanofiltration membrane and reverse osmosis membrane to concentrate; reduce water consumption, power consumption, the steam consumption significantly; improve and concentrate yield; normal temperature running environment based on membrane module; Plant hormones regulators,gibberellins thermo-sensitivity mainly contains effective constituent GA3 and obtains safeguard protection; Plant hormones regulators,gibberellins product G A3 content is whole to be improved, and the former medicine powder color and luster whiteness grade that rises has been saved the consumption of extraction agent when the concentrated solution that obtains carries out solvent extraction again.
Embodiment
Be described further below in conjunction with the present invention of embodiment.
Embodiment one.
60 tons of gibberellin fermented liquid are through Plate Filtration, obtain 50 tons of sheet frame filtrates, 18 tons of sheet frame washing lotions, sheet frame filtrate and sheet frame washing lotion after the flat plate ultrafiltration membrane of 50,000 molecular weight cut-offs filters, get 67 tons of ultrafiltrateds, 1 ton of ultrafiltration and concentration liquid again, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, get a small amount of ultrafiltrated, ultrafiltrated concentrates 67 tons of ultrafiltrateds through the one-level rolling nanofiltration membrane of 200 molecular weight cut-offs, obtains 22 tons of one-level concentrated solutions, two-pass reverse osmosis film through 80 molecular weight cut-offs concentrates, obtain 6.5 tons of final concentrated solutions of Plant hormones regulators,gibberellins, the concentrated solution yield reaches 98.1%, 16 hours working hours, power consumption 5920KWH, 20 tons of water consumptions, 2 tons of steam consumptions, Plant hormones regulators,gibberellins crystalline product GA3 content 87.5%, improve 3.1% than thin film concentration traditional technology concentrated solution yield, water consumption reduces 95%, and power consumption reduces 75%, and consumption vapour reduces 99%.Increase economic benefit 11.4%; The former medicine powder GA3 of Plant hormones regulators,gibberellins improves 3.5%.
Embodiment two.
120 tons of gibberellin fermented liquid are through Plate Filtration, obtain 100 tons of sheet frame filtrates, 36 tons of sheet frame washing lotions, sheet frame filtrate and sheet frame washing lotion are again behind the rolling ultrafiltration membrance filter of 100,000 molecular weight cut-offs, get 134 tons of ultrafiltrateds, 2 tons of ultrafiltration and concentration liquid, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, get a small amount of ultrafiltrated, ultrafiltrated concentrates 134 tons of ultrafiltrateds through the one-level rolling nanofiltration of 300 molecular weight cut-offs, obtain 44 tons of one-level concentrated solutions, concentrate through the secondary tubular type nanofiltration membrane of 100 molecular weight cut-offs, obtain 13 tons of final concentrated solutions of Plant hormones regulators,gibberellins.
Embodiment three.
120 tons of gibberellin fermented liquid are through Plate Filtration, obtain 100 tons of sheet frame filtrates, 36 tons of sheet frame washing lotions, sheet frame filtrate and sheet frame washing lotion are again after the tubular ultra-filtration membrane of 10,000 molecular weight cut-offs filters, get 134.2 tons of ultrafiltrateds, 1.8 tons of ultrafiltration and concentration liquid, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, get a small amount of ultrafiltrated, ultrafiltrated concentrates 134.2 tons of ultrafiltrateds through the one-level rolling nanofiltration of 100 molecular weight cut-offs, obtain 43.5 tons of one-level concentrated solutions, concentrate through the secondary tubular type nanofiltration membrane of 300 molecular weight cut-offs, obtain 13.3 tons of final concentrated solutions of Plant hormones regulators,gibberellins.
Claims (4)
1. the membrane filtration of a Plant hormones regulators,gibberellins, concentration technology is characterized in that may further comprise the steps successively:
(1) earlier with gibberellin fermented liquid through Plate Filtration, obtain sheet frame filtrate and sheet frame washing lotion, sheet frame filtrate and sheet frame washing lotion get ultrafiltrated and ultrafiltration and concentration liquid after flat plate ultrafiltration membrane filters again, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, and gets ultrafiltrated;
(2) ultrafiltrated is adopted the rolling nanofiltration membrane carry out one-level and concentrate 3-5v/v, adopt reverse osmosis membrane to carry out secondary and concentrate 1.5-3v/v, get concentrated solution; The mould material of described rolling nanofiltration membrane is a kind of in polysulfone membrane, polyethylene film, poly (ether sulfone) film, cellulose acetate film, polyvinylidene fluoride film, polyamide membrane or the composite membrane;
The specification of flat plate ultrafiltration membrane described in the step (1) is 1-10 ten thousand molecular weight cut-offs;
The specification of rolling nanofiltration membrane is the 100-300 molecular weight cut-off described in the step (2).
2. the membrane filtration of a kind of Plant hormones regulators,gibberellins according to claim 1, concentration technology, it is characterized in that: the described flat plate ultrafiltration membrane of step (1) is replaced with rolling ultra-filtration membrane or tubular ultra-filtration membrane, and mould material is a kind of in polysulfone membrane, polyethylene film, poly (ether sulfone) film, cellulose acetate film, polyvinylidene fluoride film, polyamide membrane or the composite membrane.
3. the membrane filtration of a kind of Plant hormones regulators,gibberellins according to claim 2, concentration technology, it is characterized in that: the specification of rolling ultra-filtration membrane and tubular ultra-filtration membrane described in the step (1) is 1-10 ten thousand molecular weight cut-offs.
4. the membrane filtration of a kind of Plant hormones regulators,gibberellins according to claim 1, concentration technology, it is characterized in that: the specification of reverse osmosis membrane is the 50-100 molecular weight cut-off described in the step (2).
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110190855 CN102241653B (en) | 2011-07-08 | 2011-07-08 | Membrane filtration and concentration technology of gibberellin |
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| CN 201110190855 CN102241653B (en) | 2011-07-08 | 2011-07-08 | Membrane filtration and concentration technology of gibberellin |
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| CN102241653A CN102241653A (en) | 2011-11-16 |
| CN102241653B true CN102241653B (en) | 2013-09-25 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102775372A (en) * | 2012-07-19 | 2012-11-14 | 浙江钱江生物化学股份有限公司 | Application method of reverse osmose membrane technology in gibberellin extraction |
| CN103360355B (en) * | 2012-12-28 | 2015-10-21 | 三达膜科技（厦门）有限公司 | The method of Plant hormones regulators,gibberellins is extracted from fermented liquid |
| CN103641868B (en) * | 2013-11-18 | 2015-12-09 | 宁夏泰瑞制药股份有限公司 | A kind of method utilizing daunorubicin fermentation liquor to produce daunorubicin hydrochloride |
| CN105541603B (en) * | 2015-12-23 | 2019-04-19 | 江西新瑞丰生化有限公司 | A method of separating natural falls off acid crude from abscisic acid fermentation liquid |
| CN105622559A (en) * | 2016-02-03 | 2016-06-01 | 昆山东大智汇技术咨询有限公司 | Gibberellin fermentation liquid membrane extraction water crystallization process |
| CN106749140A (en) * | 2017-01-23 | 2017-05-31 | 同舟纵横（厦门）流体技术有限公司 | A kind of gibberellin high power concentration technology and concentration systems |
| CN108176226A (en) * | 2018-01-18 | 2018-06-19 | 四川龙蟒福生科技有限责任公司 | The membrane filtration method for concentration of gibberellic acid and membrane filtration concentration systems |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1055738A (en) * | 1990-04-16 | 1991-10-30 | 中外合资福州榕山生化制品开发有限公司 | Gibberellin extraction process |
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Address after: Ji'an City Chengnan Industrial Zone 331300 Jiangxi Xingan Patentee after: Jiangxi new Ruifeng biochemical Limited by Share Ltd Address before: Ji'an City Chengnan Industrial Zone 331300 Jiangxi Xingan Patentee before: Jiangxi Xinruifeng Biochemical Co., Ltd. |