CN107473979A - A kind of processing method of film extraction theanine - Google Patents
A kind of processing method of film extraction theanine Download PDFInfo
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- CN107473979A CN107473979A CN201710715706.XA CN201710715706A CN107473979A CN 107473979 A CN107473979 A CN 107473979A CN 201710715706 A CN201710715706 A CN 201710715706A CN 107473979 A CN107473979 A CN 107473979A
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- extraction
- tealeaves
- theanine
- film
- processing method
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C231/00—Preparation of carboxylic acid amides
- C07C231/22—Separation; Purification; Stabilisation; Use of additives
- C07C231/24—Separation; Purification
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- Organic Chemistry (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of processing method of film extraction theanine, it is characterised in that including following operating procedure：Step 1, fresh tea leaf is put into digester and extracted, the leaching liquor after extraction is crossed into 15 25 mesh sieves, obtains tealeaves filtrate；Step 2, obtained filtrate is removed macromolecular substances with composite tube type milipore filter and handled, obtains tealeaves refined filtration liquid；Step 3, obtained tealeaves refined filtration liquid is subjected to concentration with concentration film, obtains concentrate；Step 4, obtained concentrate addition extractant is extracted, takes extraction heavy phase；Step 5, obtained extraction heavy phase is subjected to separating treatment with milipore filter, obtains separating liquid；Step 6, obtained separating liquid is subjected to adsorption treatment with macroporous absorbent resin, obtain theanine extract, The present invention reduces a large amount of reagents needed for traditional extraction technique, using membrane processing method, ecological, environmental protective, energy consumption are small, DNA purity is high, technique is simple, production cost is low, and separative efficiency is high.
Description
Technical field
The invention belongs to membrane separation concentration technical field, and in particular to a kind of processing method of film extraction theanine.
Background technology
With continually developing for tealeaves, with characteristics such as its health cares constantly by it is found that and increasingly by the green grass or young crops of people
Look at, on the premise of tealeaves active component is not destroyed, the extraction to active ingredient in tealeaves has become the trend that plant lifts；
Theanine is distinctive free amino acid in tealeaves, and pleasantly sweet, theanine content changes because of the kind of tea, position, theanine
The 1-2% of weight is accounted in dry tea, theanine is similar to intracerebral active material glutamine, glutamic acid on chemical constitution, is tealeaves
In promote the production of body fluid profit sweet tea Main Ingredients and Appearance, theanine content is about 1-2% of newly picked and processed tea leaves or so, and its content is reduced with fermentation process；Theanine
It can reduce blood pressure, enhancing memory and other effects.
Currently conventional theanine preparation method has chemical synthesis, microbe fermentation method, enzyme process, tea callus synthesis
Method and tealeaves extraction method etc., domestic and international theanine chemical synthesis are mainly poisonous former with inflammability using ethamine or triethylamine etc.
Material, can bring the misgivings of security, the synthetic route also runs counter to Green Chemistry principle, using life in food or field of medicaments application
The method cost being combined to is higher, and is a green energy conservation technology of rising in recent years using membrane separation concentration technology, operation
Simple cost is low.
The content of the invention
It is an object of the invention to provide it is a kind of it is simple to operate, cost is low, separative efficiency is high, the film of energy-conserving and environment-protective extraction tea
The processing method of propylhomoserin.
The technical scheme is that a kind of processing method of film extraction theanine, including it is extraction coarse filtration, milipore filter refined filtration, dense
Six concentration of contracting film, solvent extraction, Ultra filtration membrane and resin adsorption steps, concrete operation step are as follows：
Step 1：Fresh tea leaf is put into digester, adds purifying water termostat immersion 2-3 hour of the temperature more than 80 DEG C, will
Leaching liquor after extraction crosses 15-25 mesh sieves, obtains tealeaves filtrate；
Step 2：Obtained filtrate is subjected to refined filtration processing with composite tube type milipore filter, removes the macromolecular complex in tealeaves filtrate
Matter, obtain tealeaves refined filtration liquid；
Step 3：Obtained tealeaves refined filtration liquid is subjected to concentration with concentration film, concentrated tealeaves refined filtration liquid with reverse osmosis membrane,
Thickening temperature is 35 DEG C -55 DEG C, obtains concentrate；
Step 4：Obtained concentrate addition extractant is extracted, takes extraction heavy phase；
Step 5：Obtained extraction heavy phase is subjected to separating treatment with milipore filter, obtains separating liquid；
Step 6：Obtained separating liquid is subjected to adsorption treatment with macroporous absorbent resin, the organic substance in adsorbing separation liquid is right
Separating liquid carries out separating-purifying, obtains theanine extract.
Preferably, macromolecular substances described in step 2 include protein or polysaccharide.
Preferably, the weight of fresh tea leaf described in step 1 and purified water ratio is 1:5-8.
Preferably, it is ethyl acetate solution to be extracted described in step 4, and the volume ratio of the concentrate and ethyl acetate is
1.2:1。
Preferably, the rejection of reverse osmosis membrane is more than 95% in step 3.
Preferably, milipore filter described in step 2 is ceramic membrane, and membrane aperture 50nm, ultrafiltration pressure is 0.15-0.3MPa.
The present invention is a kind of processing method of film extraction theanine, dense by extracting coarse filtration, milipore filter refined filtration, concentration film
Six contracting, solvent extraction, Ultra filtration membrane and resin adsorption steps extract theanine from tealeaves, using milipore filter pair
Filtrate carries out refined filtration, and reverse osmosis membrane is concentrated to tealeaves refined filtration liquid and milipore filter extraction heavy phase stripped to solution is divided
From processing, adsorption treatment finally is carried out with macroporous absorbent resin, obtains theanine.
Beneficial effects of the present invention：
The present invention is a kind of processing method of film extraction theanine, reduces a large amount of reagents needed for traditional extraction technique, adopts
With membrane processing method, ecological, environmental protective, energy consumption are small, DNA purity is high, technique is simple, production cost is low, and separative efficiency is high.
Brief description of the drawings
Fig. 1 is the operational flowchart for the processing method that a kind of film of the present invention extracts theanine.
Embodiment
For ease of skilled artisan understands that the present invention program, makees in conjunction with Figure of description to technical solution of the present invention
It is further elaborated with.
Embodiment 1
As shown in figure 1, the present invention is a kind of processing method of film extraction theanine, including extraction coarse filtration, milipore filter refined filtration, concentration
Six film concentration, solvent extraction, Ultra filtration membrane and resin adsorption steps, concrete operation step are as follows：
Step 1：Fresh tea leaf is put into digester, purifying water termostat of the temperature more than 80 DEG C is added and soaks 2 hours, will soak
Leaching liquor after carrying crosses 15 mesh sieves, obtains tealeaves filtrate；
Step 2：Obtained filtrate is subjected to refined filtration processing with composite tube type milipore filter, removes the macromolecular complex in tealeaves filtrate
Matter, obtain tealeaves refined filtration liquid；
Step 3：Obtained tealeaves refined filtration liquid is subjected to concentration with concentration film, concentrated tealeaves refined filtration liquid with reverse osmosis membrane,
Thickening temperature is 35 DEG C, obtains concentrate；
Step 4：Obtained concentrate addition extractant is extracted, takes extraction heavy phase；
Step 5：Obtained extraction heavy phase is subjected to separating treatment with milipore filter, obtains separating liquid；
Step 6：Obtained separating liquid is subjected to adsorption treatment with macroporous absorbent resin, the organic substance in adsorbing separation liquid is right
Separating liquid carries out separating-purifying, obtains theanine extract.
Macromolecular substances described in step 2 include protein or polysaccharide.Fresh tea leaf described in step 1 and purified water
Weight ratio is 1:5.It is ethyl acetate that solution is extracted described in step 4, and the volume ratio of the concentrate and ethyl acetate is 1.2:
1.The rejection of reverse osmosis membrane is more than 95% in step 3.Milipore filter described in step 2 is ceramic membrane, membrane aperture 50nm, is surpassed
Filtering pressure is 0.15MPa by force.
Embodiment 2
As shown in figure 1, the present invention is a kind of processing method of film extraction theanine, including extraction coarse filtration, milipore filter refined filtration, concentration
Six film concentration, solvent extraction, Ultra filtration membrane and resin adsorption steps, concrete operation step are as follows：
Step 1：Fresh tea leaf is put into digester, purifying water termostat of the temperature more than 80 DEG C is added and soaks 3 hours, will soak
Leaching liquor after carrying crosses 25 mesh sieves, obtains tealeaves filtrate；
Step 2：Obtained filtrate is subjected to refined filtration processing with composite tube type milipore filter, removes the macromolecular complex in tealeaves filtrate
Matter, obtain tealeaves refined filtration liquid；
Step 3：Obtained tealeaves refined filtration liquid is subjected to concentration with concentration film, concentrated tealeaves refined filtration liquid with reverse osmosis membrane,
Thickening temperature is 55 DEG C, obtains concentrate；
Step 4：Obtained concentrate addition extractant is extracted, takes extraction heavy phase；
Step 5：Obtained extraction heavy phase is subjected to separating treatment with milipore filter, obtains separating liquid；
Step 6：Obtained separating liquid is subjected to adsorption treatment with macroporous absorbent resin, the organic substance in adsorbing separation liquid is right
Separating liquid carries out separating-purifying, obtains theanine extract.
Macromolecular substances described in step 2 include protein or polysaccharide.Fresh tea leaf described in step 1 and purified water
Weight ratio is 1:8.It is ethyl acetate that solution is extracted described in step 4, and the volume ratio of the concentrate and ethyl acetate is 1.2:
1.The rejection of reverse osmosis membrane is more than 95% in step 3.Milipore filter described in step 2 is ceramic membrane, membrane aperture 50nm, is surpassed
Filtering pressure is 0.3MPa by force.
The present invention is a kind of processing method of film extraction theanine, and extraction coarse filtration is crossed using milipore filter and reverse osmosis membrane
Tealeaves filtrate carries out refined filtration and concentration, then adds solvent in concentrate and extracted, and then extraction heavy phase is entered with milipore filter
Row separating and filtering, suction-operated finally is carried out with macroporous absorbent resin, obtain the extract of theanine.
Invention is exemplarily described above in conjunction with accompanying drawing by the present invention program, it is clear that present invention specific implementation is not
Limited by aforesaid way, as long as the various unsubstantialities for employing inventive concept and technical scheme of the present invention progress improve,
Or it is not improved the design of invention and technical scheme are directly applied into other occasions, protection scope of the present invention it
It is interior.
Claims (6)
1. a kind of processing method of film extraction theanine, it is characterised in that dense including extraction coarse filtration, milipore filter refined filtration, concentration film
Six contracting, solvent extraction, Ultra filtration membrane and resin adsorption steps, concrete operation step are as follows：
Step 1：Fresh tea leaf is put into digester, adds purifying water termostat immersion 2-3 hour of the temperature more than 80 DEG C, will
Leaching liquor after extraction crosses 15-25 mesh sieves, obtains tealeaves filtrate；
Step 2：Obtained filtrate is subjected to refined filtration processing with composite tube type milipore filter, removes the macromolecular complex in tealeaves filtrate
Matter, obtain tealeaves refined filtration liquid；
Step 3：Obtained tealeaves refined filtration liquid is subjected to concentration with concentration film, concentrated tealeaves refined filtration liquid with reverse osmosis membrane,
Thickening temperature is 35 DEG C -55 DEG C, obtains concentrate；
Step 4：Obtained concentrate addition extractant is extracted, takes extraction heavy phase；
Step 5：Obtained extraction heavy phase is subjected to separating treatment with milipore filter, obtains separating liquid；
Step 6：Obtained separating liquid is subjected to adsorption treatment with macroporous absorbent resin, the organic substance in adsorbing separation liquid is right
Separating liquid carries out separating-purifying, obtains theanine extract.
2. the processing method of a kind of film extraction theanine according to claim 1, it is characterised in that big described in step 2
Molecular substance includes protein or polysaccharide.
3. the processing method of a kind of film extraction theanine according to claim 1, it is characterised in that new described in step 1
The weight of fresh tea passes and purified water ratio is 1:5-8.
4. the processing method of a kind of film extraction theanine according to claim 1, it is characterised in that extract described in step 4
It is ethyl acetate to take solution, and the volume ratio of the concentrate and ethyl acetate is 1.2:1.
A kind of 5. processing method of film extraction theanine according to claim 1, it is characterised in that counter-infiltration in step 3
The rejection of film is more than 95%.
6. the processing method of a kind of film extraction theanine according to claim 1, it is characterised in that surpass described in step 2
Filter membrane is ceramic membrane, and membrane aperture 50nm, ultrafiltration pressure is 0.15-0.3MPa.
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| CN201710715706.XA CN107473979A (en) | 2017-08-20 | 2017-08-20 | A kind of processing method of film extraction theanine |
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| CN201710715706.XA CN107473979A (en) | 2017-08-20 | 2017-08-20 | A kind of processing method of film extraction theanine |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527147A (en) * | 2018-12-18 | 2019-03-29 | 合肥信达膜科技有限公司 | A kind of membrane separation technique of tea comprehensive processing |
| CN112640972A (en) * | 2019-10-11 | 2021-04-13 | 李彤 | Preparation method of high-concentration theanine tea |
| CN113350322A (en) * | 2020-03-06 | 2021-09-07 | 李彤 | Multifunctional aromatherapy liquid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106518710A (en) * | 2016-09-23 | 2017-03-22 | 合肥信达膜科技有限公司 | Membrane treatment method for extracting theanine from tea |
-
2017
- 2017-08-20 CN CN201710715706.XA patent/CN107473979A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106518710A (en) * | 2016-09-23 | 2017-03-22 | 合肥信达膜科技有限公司 | Membrane treatment method for extracting theanine from tea |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527147A (en) * | 2018-12-18 | 2019-03-29 | 合肥信达膜科技有限公司 | A kind of membrane separation technique of tea comprehensive processing |
| CN112640972A (en) * | 2019-10-11 | 2021-04-13 | 李彤 | Preparation method of high-concentration theanine tea |
| CN113350322A (en) * | 2020-03-06 | 2021-09-07 | 李彤 | Multifunctional aromatherapy liquid |
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