CN102227117A - Monitoring system for cells under deep hypothermia refrigeration - Google Patents
Monitoring system for cells under deep hypothermia refrigeration Download PDFInfo
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Abstract
The invention discloses a monitoring system for cells under deep hypothermia refrigeration. The monitoring system comprises a data acquisition layer, a data transmission layer, a data processing layer and an information service layer, wherein the data processing layer utilizes a low-temperature sensing device to monitor the environment condition and provides data to the data transmission layer; the data transmission layer transmits data to the data processing layer by utilizing the radio frequency technology and the network technology; a server processes the data and provides the processed data to the information service layer, and meanwhile, a client can access the server to acquire the environment information of cells. The monitoring system for cells under deep hypothermia refrigeration provided by the invention can realize the beyond-visual-range measurement, monitor, inspection tour, alarm and quality status analysis on the temperature in real time on the basis of a higher accuracy level through non-contact and high-accuracy temperature measurement, remote centre storage, data analysis and remote networking, thus achieving the purpose of modernized monitoring with objective and reliable data, retrospective quality accidents, and non-contact beyond-visual-range real-time temperature monitoring for cells.
Description
Technical field
The invention belongs to the refrigeration field, relate in particular to a kind of monitoring system of cell profound hypothermia cold storage environment.
Background technology
Current, along with the high speed development of IT technology, radio-frequency communication technology, internet communication technology, digitlization observation and control technology, a brand-new rising industry---Internet of Things is just grown up fast in all trades and professions.
Cell technology, the high-end professional technique as association areas such as modern disease control, medical treatment, military affairs, scientific researches has become one of most advanced and sophisticated professional technique that influences national economy.Because cell technology is for the specific dependency of environment, cytoactive has special, professional requirement to environmental key-elements such as temperature, humidity, in the links such as the sampling of cell products, exploitation, production, transportation and storage, the key element that influences cytoactive for temperature, humidity etc. has extremely urgent, strict measurement and monitoring requirement.
Glassy state is a kind of existence of material, the material of this moment has certain shape and volume as solid, arrangement between the liquid molecule is just in order approximate again, is amorphous state or amorphous state therefore, is similar to the light transmissive material glass that we know; The sub-chain motion of macromolecule polyalcohol that is in this state is frozen, only allows undersized motion, so its deformation is very little, therefore is called glassy state; And change softness into when macromolecule polyalcohol and during the rubber-like solid, just be in rubbery state.So-called amorphous (Amorphous) is meant residing a kind of non-equilibrium, the non-crystalline state of material, preponderates and solute when keeping noncrystalline when saturation conditions, and the solid that form this moment is exactly an amorphous state; (Glass transitiontemperature Tg), is meant the temperature (as shown in Figure 1) when the transformation (glass transition) of amorphous food from the glassy state to the rubbery state to so-called vitrification point.
The biological material vitrified low temperature that grows up the eighties in 20th century is preserved; be cell and protective agent solution thereof to be crossed with enough fast rate of temperature fall be as cold as its glass transition temperature, and be cured as completely glassy state and with this glassy state method of long preservation at low temperatures.Avoid crystallization in this course inside and outside the cell fully, thereby avoided the various damages cause owing to freezing.People such as Papatz adopt the glycerine protective agent to realize the vitrifying of red blood cell suspension.People such as Fahy have inquired into the basic principle that vitrifying is preserved, and have described and how to have realized vitrified basic process.People's such as Rall mouse embryo vitrifying is preserved and has been obtained success.People such as Fuku have carried out the research of Change of Ultrastructure in vitrifying preservation and the preservation process to the egg mother cell of ox.
The profound hypothermia vitrifying storage of cell is the most advanced memory technology of current practicality, and its key element is that rate temperature change and temperature are steadily monitored.
Current, in exploitation, production, transportation and the storage process of cell products, a kind of method is to adopt the living environment of the mode pair cell of manual measurement, paper record to monitor, this class methods manpower consumption is big, measure error varies with each individual, immense papery data retrieval and analysis are difficult, for some cells (virus) with human body invasive, can't effectively reduce or remit the harm of the person.Second method is by using special equipment to carry out individuation, discrete preservation and monitoring, these class methods increase on the measurement consistency, but still need manual record and analysis, in addition because the stationarity and the restricted number of its sampled point do not possess flexibility in the practical application.
Summary of the invention
The objective of the invention is to develop that a kind of measuring point is disposed flexibly, measure error unanimity, deduction and exemption personnel contact, automation is monitored in real time and the temperature patrol inspection that can built-in default rule and the cell profound hypothermia refrigeration monitoring system of warning.
The technical solution adopted in the present invention is:
A kind of cell profound hypothermia refrigeration monitoring system that is used to monitor cellular environment, it comprises data collection layer, data transfer layer, data analysis layer and information service layer, wherein data analysis layer utilizes the low temperature sensing device that ambient condition is monitored and provides data to data transfer layer, data transfer layer utilize radio-frequency technique and network technology with transfer of data to data analysis layer, data are handled and data are offered information service layer by server.
Described data collection layer comprises a plurality of profound hypothermia temperature sensing devices, described profound hypothermia temperature sensing device comprises the temperature sensor of direct detection ambient temperature, be connected with described temperature sensor and obtain its temperature data temperature data control device that line data handles of going forward side by side, described temperature data control device comprises the housing that constitutes its outward appearance, be arranged on the mainboard of its enclosure interior and can give the power supply device of described mainboard energy supply, the single-chip microcomputer that described mainboard comprises, wherein said single-chip microcomputer has the profound hypothermia temperature compensation unit, the storage flash chip of real time temperature data, improve the radio frequency chip of radiofrequency signal and provide I for each chip unit to profound hypothermia temperature hub
2The I of C read-write bus
2The C chip.
Preferably, can also adopt wireless communication mode between the profound hypothermia temperature sensor of profound hypothermia temperature sensing device and profound hypothermia temperature sensing device mainboard, be that the profound hypothermia temperature sensor is a radio temperature sensor, described profound hypothermia temperature sensing device mainboard comprises the temperature data wireless receiving module of coupling.
Described data transfer layer comprises a plurality of profound hypothermia temperature hubs, the corresponding a plurality of profound hypothermia temperature sensing devices of each profound hypothermia temperature hub, described profound hypothermia temperature hub comprises housing, be arranged on the antenna and the mainboard and the mainboard power supply device that are arranged on enclosure interior of outside, described mainboard comprises main control chip, in order to receive the temperature sensing device radiofrequency signal and signal to be transferred to the concurrent Receiver Module of multiple spot of main control chip, in order to the GPS module of the current position of profound hypothermia temperature hub information to be provided, be connected and in real time temperature data and gps data be sent to the mixed-media network modules mixed-media of data analysis layer with described main control chip, the big capacity memory chip of data storage function is provided for the master control chip.
Preferably, described data analysis layer adopts the B/S framework, and it comprises web server, the database server and the prior-warning device that are connected with the web server respectively.And patrol and examine rule at the cell temperature that the web server memory contains customization, when reaching early warning or alert if, the web server then carries out early warning, warning by prior-warning device, carries out early warning initiatively by setting rule, improves fail safe.
The invention has the beneficial effects as follows: can accomplish to measure by contactless, high-precision temperature by cell profound hypothermia refrigeration monitoring system of the present invention, remote centre storage, data analysis and long-distance on-line, reaching on the basis of high level of accuracy more, realizing that the real time temperature of over the horizon is measured, monitor, patrol and examine, warning and quality state analysis.Reach the state-of-the-art supervision purpose that data are objective reliably, quality accident can be recalled, the real-time cell temperature of contactless over the horizon is monitored.
Description of drawings
Fig. 1 is the structured flowchart of cell profound hypothermia refrigeration monitoring system of the present invention;
Fig. 2 is the structured flowchart of temperature sensing device of the present invention;
Fig. 3 is a temperature sensing device thermometric schematic diagram of the present invention;
Fig. 4 is a cryogenic temperature hub architecture block diagram of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments the present invention is described in further detail:
As shown in Figure 1, Fig. 1 is a system architecture diagram of the present invention, cell profound hypothermia monitoring system of the present invention comprises data collection layer, data transfer layer, data analysis layer and information service layer, wherein data analysis layer mainly is to adopt sensing device pair cell state to monitor, data transfer layer utilizes radio-frequency technique and radio network technique to finish the transmission of image data, transfer of data is to data analysis layer, data are handled and data are offered information service layer by server, for it provides information inquiry.
1, data collection layer
Shown in Fig. 2,3, data collection layer of the present invention comprises a plurality of profound hypothermia temperature sensing devices 100, described profound hypothermia temperature sensing device 100 comprises: profound hypothermia temperature sensor 101, the temperature data control device 108 that is connected with described profound hypothermia temperature sensor 101 and the transmitting antenna 107 that is arranged on temperature data control device 108 outsides.
Described temperature data control device 108 comprises the housing that constitutes its outward appearance and is arranged on the mainboard 102 of its enclosure interior and can gives the battery of described mainboard 102 energy supplies.Described mainboard 102 comprises the single-chip microcomputer 103 with profound hypothermia temperature compensation unit, as the C8051F410 type; Flash chip 105, in program control single-chip microcomputer 103 being handled the temperature data that obtains writes and is stored in the flash chip 105 so that as the built-in database of record queries afterwards of a part, described flash chip is selected a lot, as 16 megabit SPI serial flash chip SST25VF016B; Can be simultaneously just single-chip microcomputer 103 handle the temperature data that obtains and be sent to the radio frequency chip 106 of the profound hypothermia hub that describes below by wireless mode, wherein single-chip microcomputer 103 can adopt ADF7020-1BCPZ; And I
2C chip 104, it provides I for each chip unit
2C reads and writes bus, selectable I
2The C chip type is a lot, as A8563.
Described temperature sensor 101 is connected with single-chip microcomputer 103 data, and preferably, 102 of described profound hypothermia temperature sensor 101 and mainboards are connected by the monobus communication interface.
But described energy supply battery adopts the battery of recharge, to prolong the useful life of battery, can guarantee the miniaturization of whole temperature data control device ease of use and volume simultaneously.
Required profound hypothermia temperature-measuring range is big because cell is stored in a warehouse environment; Adopt the radio-frequency transmissions of data, reduced the constraint of data wire physics and distance, effectively can dispose the profound hypothermia temperature sensing device arbitrarily at profound hypothermia refrigerator or profound hypothermia liquid nitrogen container in the receiving range; But and function battery recharge, recharge is more than 500 times, and utilizes special intelligent battery administrative skill, and not power consumption pond electric weight when external power is arranged further prolongs useful life of rechargeable battery; Measurement data transmits after compensating unit is handled again, the certainty of measurement height, and measure error is smaller or equal to 0.5 ℃; Measurement consistency height; Real-time, time-delay is less than 1S; The thermometric adaptive capacity to environment is strong; It is convenient to safeguard, can charge repeatedly; Install or charge and need not to destroy the cell memory device.That cell profound hypothermia of the present invention refrigeration monitoring system can reach fully is contactless under the cell profound hypothermia storage condition, high-precision temperature is measured, installation and maintenance purpose easily, has great Practical significance and social effect.
2, data transfer layer
Data transfer layer of the present invention comprises a plurality of profound hypothermia temperature hubs 200; the all corresponding a plurality of profound hypothermia temperature sensing devices 100 of each profound hypothermia temperature sensor 200; as shown in Figure 4; described profound hypothermia temperature hub 200 comprises: constitute the hub outward appearance and protect the housing of internal structure, be arranged on the antenna of outside and be arranged on the mainboard 210 and the mainboard power supply device of enclosure interior.Described mainboard 210 comprises the main control chip 201 as main control unit, S3C2410 type as ARM, in order to receive the temperature sensing device radiofrequency signal and signal to be transferred to the concurrent Receiver Module 202 of multiple spot of main control chip, the concurrent received RF module 202 of described multiple spot can receive the temperature radiofrequency signal that a plurality of temperature sensors send, as maximum can the concurrent reception of ten six temperature radio frequencies ADF7020-1BCPZ, in order to the GPS module 205 of current profound hypothermia temperature hub position information to be provided, be connected and in real time temperature data and gps data be sent to the mixed-media network modules mixed-media 203 of data analysis layer with described main control chip, described mixed-media network modules mixed-media 203 includes spider lines module such as RTL8139 network card chip or wireless-transmission network module such as GPRS unit etc., provide the big capacity memory chip 204 of data storage function to main control chip, as HY5DU561622FTP-5 type 256Mb memory chip.Wherein, described mainboard power supply device is external 12V power supply.
3, data analysis layer
Data analysis layer of the present invention adopts B/S IA frame serverPC, it comprises web server 300, the database server 320 that is connected with web server 300 respectively and prior-warning device 310, web server 300 utilizes the network receiving ability and the literacy of large server, by 10000 level temperature real-time network collection modules, temperature real-time collecting software module and proprietary big capacity storage and retrieving files system, realize stable storage, retrieval and the reporting system of vast capacity data.
For promoting the user simple application ability of complication system and the user that reduces the complex information platform are got involved difficulty, the cell temperature that also stores customizable simultaneously in the web server is patrolled and examined rule, this idiographic flow of patrolling and examining rule is to open to the user to formulate, when reaching early warning or alert if, the web server then carries out early warning, warning by prior-warning device 310, carry out early warning, the warning of forms such as acousto-optic or note by pre-defined rule, promote whole fail safe.
4, information service layer
Information service layer of the present invention comprises a plurality of clients 400, as mentioned above, the present invention adopts the B/S framework, client 400 can adopt various ways visit web server, as long as there is the computer that can surf the Net just can use, and the client zero dimension is protected, the system extension of web server is very easy to, the temperature data real-time update is fast, can inquire about real time temperature and historical temperature under the transportation attitude, can also review the cell supply chain information that inquiry is received.Simultaneously, in conjunction with GPS service module and wireless network, can realize the monitoring in all directions of omnidistance position, state.The use of B/S framework also can be carried out section processes on the client computer, thereby has alleviated load of server greatly; And increased interactivity, can carry out local real time and refresh.
In sum, cell profound hypothermia monitoring system of the present invention adopts cell profound hypothermia radio frequency temperature sensor, a plurality of profound hypothermia temperature sensors are corresponding with a profound hypothermia hub, utilize radio-frequency technique to realize the transmission of temperature data, can realize that temperature troops accurately, real-time Transmission, this system has and comprises a plurality of profound hypothermia hubs simultaneously, it is equipped with mixed-media network modules mixed-media and GPS module, can utilize network that temperature data and position data are passed to the web server, and the storage of the over capacity temperature data of server, analyze, form technology and cell temperature are are regularly patrolled and examined, temperature anomaly alert detecting and switch or SMS alarm technology make client can obtain multiple information, and the real-time monitored cellular environment changes.
Can accomplish to measure by the present invention by contactless, high-precision temperature, remote centre storage, data analysis and long-distance on-line, reaching on the basis of high level of accuracy more, realizing that the real time temperature of over the horizon is measured, monitor, patrol and examine, warning and quality state analysis.Reach the state-of-the-art supervision purpose that data are objective reliably, quality accident can be recalled, the real-time cell temperature of contactless over the horizon is monitored.
The above instrument is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. cell profound hypothermia refrigeration monitoring system that is used to monitor cellular environment, it is characterized in that: it comprises data collection layer, data transfer layer, data analysis layer and information service layer, wherein data analysis layer utilizes profound hypothermia sensing device pair cell ambient condition to monitor and provides data to data transfer layer, data transfer layer utilize radio-frequency technique and network technology with transfer of data to data analysis layer, data are handled and data are offered information service layer by server.
2. cell profound hypothermia refrigeration monitoring system as claimed in claim 1, it is characterized in that: described data collection layer comprises a plurality of profound hypothermia temperature sensing devices, described profound hypothermia temperature sensing device comprises the profound hypothermia temperature sensor (101) of direct detection ambient temperature, be connected with described profound hypothermia temperature sensor (101) and obtain its temperature data temperature data control device (108) that line data handles of going forward side by side, described temperature data control device (108) comprises the housing that constitutes its outward appearance, be arranged on the mainboard (102) of its enclosure interior and can give the power supply device of described mainboard (102) energy supply, the single-chip microcomputer (103) that described mainboard (102) comprises, the storage flash chip (105) of real time temperature data, improve the radio frequency chip (106) of radiofrequency signal and provide I for each chip unit to profound hypothermia temperature hub
2The I of C read-write bus
2C chip (104).
3. cell profound hypothermia refrigeration monitoring system as claimed in claim 2, it is characterized in that: described single-chip microcomputer has the profound hypothermia temperature compensation unit.
4. cell profound hypothermia refrigeration monitoring system as claimed in claim 2, it is characterized in that: described temperature sensor is a radio temperature sensor, described mainboard comprises the temperature data wireless receiving module of coupling
5. cell profound hypothermia as claimed in claim 2 refrigeration monitoring system is characterized in that: be connected by the monobus communication interface between described temperature sensor (1) and mainboard (2).
6. as the described cell profound hypothermia refrigeration of claim 1-5 monitoring system, it is characterized in that: described data transfer layer comprises a plurality of profound hypothermia temperature hubs, the corresponding a plurality of profound hypothermia temperature sensors of each profound hypothermia temperature hub, described profound hypothermia temperature hub comprises housing, be arranged on the antenna and the mainboard (210) and the mainboard power supply device that are arranged on enclosure interior of outside, described mainboard (210) comprises main control chip (201), in order to radiofrequency signal that receives profound hypothermia temperature sensing device (100) and the Receiver Module (202) that signal is transferred to main control chip (201), in order to the GPS module (205) of the current position of profound hypothermia temperature hub (200) information to be provided, the mixed-media network modules mixed-media (203) that is connected and in real time data is sent to data analysis layer with described main control chip, the big capacity memory chip (204) of data storage function is provided for the master control chip.
7. cell profound hypothermia refrigeration monitoring system as claimed in claim 6, it is characterized in that: described Receiver Module is the concurrent Receiver Module of wireless multipoint.
8. cell profound hypothermia refrigeration monitoring system as claimed in claim 1, it is characterized in that: described data analysis layer adopts the B/S framework, and it comprises web server, the database server and the prior-warning device that are connected with the web server respectively.
9. cell profound hypothermia refrigeration monitoring system as claimed in claim 8, it is characterized in that: contain cell temperature in the web server memory and patrol and examine rule, when reaching early warning or alert if, the web server then carries out early warning, warning by prior-warning device.
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| CN103823497A (en) * | 2014-03-04 | 2014-05-28 | 浙江大学宁波理工学院 | Device for on-line monitoring and controlling low-temperature protective agent solution for treating biological tissues |
| CN106094777A (en) * | 2016-08-26 | 2016-11-09 | 北京航空航天大学 | A kind of industry internet Hub device |
| CN106885641A (en) * | 2016-12-31 | 2017-06-23 | 新昌县迪斯曼科技有限公司 | Monitoring system for cells under deep hypothermia refrigeration |
| CN107318827A (en) * | 2017-07-03 | 2017-11-07 | 上海理工大学 | A kind of medical speed governing heat sink with communication function |
| CN110519985A (en) * | 2017-03-31 | 2019-11-29 | 弗劳恩霍夫应用研究促进协会 | Low temperature indicator mixture, the device and method for monitoring the temperature transition under low temperature |
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| WO2007115794A1 (en) * | 2006-04-05 | 2007-10-18 | Smartfreeze S.R.L. | A system for remotely controlling and monitoring a food refrigerator and its content |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103823497A (en) * | 2014-03-04 | 2014-05-28 | 浙江大学宁波理工学院 | Device for on-line monitoring and controlling low-temperature protective agent solution for treating biological tissues |
| CN103823497B (en) * | 2014-03-04 | 2016-02-10 | 浙江大学宁波理工学院 | Low temperature protectant solution process biological tissue's on-line monitoring control device |
| CN106094777A (en) * | 2016-08-26 | 2016-11-09 | 北京航空航天大学 | A kind of industry internet Hub device |
| CN106094777B (en) * | 2016-08-26 | 2018-03-02 | 北京航空航天大学 | A kind of industry internet Hub devices |
| CN106885641A (en) * | 2016-12-31 | 2017-06-23 | 新昌县迪斯曼科技有限公司 | Monitoring system for cells under deep hypothermia refrigeration |
| CN110519985A (en) * | 2017-03-31 | 2019-11-29 | 弗劳恩霍夫应用研究促进协会 | Low temperature indicator mixture, the device and method for monitoring the temperature transition under low temperature |
| CN107318827A (en) * | 2017-07-03 | 2017-11-07 | 上海理工大学 | A kind of medical speed governing heat sink with communication function |
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Address after: 300457 No. 220 Dongting Road, economic and Technological Development Zone, Binhai New Area, Tianjin Patentee after: Shun Hao Cell Biotechnology (Tianjin) Limited by Share Ltd Address before: 300457 No. 220 Dongting Road, economic and Technological Development Zone, Binhai New Area, Tianjin Patentee before: Shun Hao Cell Biotechnology (Tianjin) Co., Ltd. |