CN102227117B - Monitoring system for cells under deep hypothermia refrigeration - Google Patents

Monitoring system for cells under deep hypothermia refrigeration Download PDF

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CN102227117B
CN102227117B CN 201110089950 CN201110089950A CN102227117B CN 102227117 B CN102227117 B CN 102227117B CN 201110089950 CN201110089950 CN 201110089950 CN 201110089950 A CN201110089950 A CN 201110089950A CN 102227117 B CN102227117 B CN 102227117B
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data
temperature
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monitoring system
hypothermia
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CN102227117A (en
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罗元浩
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Shun Hao Cell Biotechnology (Tianjin) Limited by Share Ltd
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Shun Hao Cell Biotechnology (tianjin) Co Ltd
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Abstract

The invention discloses a monitoring system for cells under deep hypothermia refrigeration. The monitoring system comprises a data acquisition layer, a data transmission layer, a data processing layer and an information service layer, wherein the data processing layer utilizes a low-temperature sensing device to monitor the environment condition and provides data to the data transmission layer; the data transmission layer transmits data to the data processing layer by utilizing the radio frequency technology and the network technology; a server processes the data and provides the processed data to the information service layer, and meanwhile, a client can access the server to acquire the environment information of cells. The monitoring system for cells under deep hypothermia refrigeration provided by the invention can realize the beyond-visual-range measurement, monitor, inspection tour, alarm and quality status analysis on the temperature in real time on the basis of a higher accuracy level through non-contact and high-accuracy temperature measurement, remote centre storage, data analysis and remote networking, thus achieving the purpose of modernized monitoring with objective and reliable data, retrospective quality accidents, and non-contact beyond-visual-range real-time temperature monitoring for cells.

Description

Monitoring system for cells under deep hypothermia refrigeration
Technical field
The invention belongs to the refrigeration field, relate in particular to a kind of monitoring system of cell profound hypothermia cold storage environment.
Background technology
Current, along with the high speed development of IT technology, radio-frequency communication technology, internet communication technology, digitlization observation and control technology, a brand-new rising industry---Internet of Things is just at all trades and professions Fast Growth.
Cell technology, the high-end professional technique as association areas such as modern disease control, medical treatment, military affairs, scientific researches, become one of most advanced and sophisticated professional technique affected national economy.Due to the specific dependency of cell technology for environment, cytoactive has special, professional requirement to environmental key-elements such as temperature, humidity, in the links such as the sampling of cell products, exploitation, production, transportation and storage, the key element that affects cytoactive for temperature, humidity etc. has extremely urgent, strict measurement and monitoring requirement.
Glassy state is a kind of existence of material, material now has certain shape and volume as solid, just in order approximate as the arrangement between molecule liquid again, be therefore amorphous state or amorphous state, be similar to the light transmissive material glass that we know; The sub-chain motion of the macromolecule polyalcohol in this state is frozen, only allows undersized motion, so its deformation is very little, therefore is called glassy state; And when macromolecule polyalcohol changes softness into and has flexible solid, just in rubbery state.So-called amorphous (Amorphous) refers to residing a kind of non-equilibrium, the non-crystalline state of material, when saturation conditions, preponderates and solute while keeping noncrystalline, and the solid now formed is exactly amorphous state; So-called vitrification point (Glass transitiontemperature, Tg), the temperature (as shown in Figure 1) while referring to when amorphous food the transformation (glass transition) from the glassy state to the rubbery state.
The biological material vitrified low temperature grown up the eighties in 20th century is preserved; be to make cell and protective agent solution thereof cross and be as cold as its glass transition temperature with enough fast rate of temperature fall, and be cured as completely glassy state and with this glassy state method of long preservation at low temperatures.Avoid in this course crystallization inside and outside cell fully, thereby avoided the various damages that cause owing to freezing.The people such as Papatz adopt the glycerine protective agent to realize the vitrifying of red blood cell suspension.The people such as Fahy have inquired into the basic principle that vitrifying is preserved, and have described and how to have realized vitrified basic process.The people's such as Rall mouse embryo vitrifying is preserved and has been obtained success.The people such as Fuku have carried out the research of Change of Ultrastructure in vitrifying preservation and preservation process to the egg mother cell of ox.
The profound hypothermia vitrifying storage of cell, be current practicality the most advanced memory technology, and its key element is that rate temperature change and temperature are steadily monitored.
Current, in exploitation, production, transportation and the storage process of cell products, a kind of method is to adopt the mode of manual measurement, paper record to be monitored the living environment of cell, this class methods manpower consumption is large, measure error varies with each individual, immense papery data retrieval and analysis are difficult, and the cell (virus) that has the human body invasive for some, can't effectively reduce or remit personal harm.Second method is by using special equipment to carry out individuation, discrete preservation and monitoring, these class methods increase on the measurement consistency, but still need manual record and analysis, due to stationarity and the restricted number of its sampled point, do not possess flexibility in practical application in addition.
Summary of the invention
The object of the invention is to develop that a kind of measuring point is disposed flexibly, measure error consistent, deduction and exemption personnel contact, automation Real-Time Monitoring and temperature patrol inspection that can built-in default rule and the monitoring system for cells under deep hypothermia refrigeration of warning.
The technical solution adopted in the present invention is:
A kind of for monitoring the monitoring system for cells under deep hypothermia refrigeration of cellular environment, it comprises data collection layer, data transfer layer, data analysis layer and information service layer, wherein data analysis layer utilizes the low temperature sensing device ambient condition is monitored and provide data to data transfer layer, data transfer layer utilizes radio-frequency technique and network technology to send data to data analysis layer, by server, data is processed and data are offered to information service layer.
Described data collection layer comprises a plurality of ultra-low-temperature sensor devices, described ultra-low-temperature sensor device comprises the temperature sensor of direct detection ambient temperature, the temperature data control device that is connected and obtains its temperature data and carry out the data processing with described temperature sensor, described temperature data control device comprises the housing that forms its outward appearance, be arranged on the mainboard of its enclosure interior and can give the power supply device of described mainboard energy supply, the single-chip microcomputer that described mainboard comprises, wherein said single-chip microcomputer has the Profound hypothermia temperature compensating unit, the storage flash chip of real time temperature data, improve the radio frequency chip of radiofrequency signal and provide I for each chip unit to Profound hypothermia temperature hub 2the I of C read-write bus 2the C chip.
Preferably, can also adopt wireless communication mode between the Profound hypothermia temperature transducer of ultra-low-temperature sensor device and ultra-low-temperature sensor device mainboard, be that the Profound hypothermia temperature transducer is radio temperature sensor, the temperature data wireless receiving module that described ultra-low-temperature sensor device mainboard comprises coupling.
Described data transfer layer comprises a plurality of Profound hypothermia temperature hubs, the corresponding a plurality of ultra-low-temperature sensor devices of each Profound hypothermia temperature hub, described Profound hypothermia temperature hub comprises housing, be arranged on the antenna and the mainboard and the mainboard power supply device that are arranged on enclosure interior of outside, described mainboard comprises main control chip, in order to receive the temperature sensing device radiofrequency signal and signal to be transferred to the concurrent Receiver Module of multiple spot of main control chip, in order to the GPS module of the current position of Profound hypothermia temperature hub information to be provided, be connected and in real time temperature data and gps data be sent to the mixed-media network modules mixed-media of data analysis layer with described main control chip, the large capacity memory chip of data storage function is provided to Master control chip.
Preferably, described data analysis layer adopts the B/S framework, and it comprises web server, the database server be connected with web server respectively and prior-warning device.And the cell temperature that stores customization in web server patrols and examines rule, when reaching early warning or alert if, web server is carried out early warning, warning by prior-warning device, by setting rule, carries out early warning initiatively, improves fail safe.
The invention has the beneficial effects as follows: by monitoring system for cells under deep hypothermia refrigeration of the present invention, can accomplish to measure by contactless, high-precision temperature, remote centre storage, data analysis and long-distance on-line, reaching on the basis of high level of accuracy more, realizing that the real time temperature of over the horizon is measured, monitor, patrol and examine, warning and quality state analysis.Reach the state-of-the-art supervision purpose that data are objective reliably, quality accident can be recalled, the real-time cell temperature of contactless over the horizon is monitored.
The accompanying drawing explanation
The structured flowchart that Fig. 1 is monitoring system for cells under deep hypothermia refrigeration of the present invention;
The structured flowchart that Fig. 2 is temperature sensing device of the present invention;
Fig. 3 is temperature sensing device thermometric schematic diagram of the present invention;
Fig. 4 is cryogenic temperature hub architecture block diagram of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail:
As shown in Figure 1, Fig. 1 is system architecture diagram of the present invention, cell profound hypothermia monitoring system of the present invention comprises data collection layer, data transfer layer, data analysis layer and information service layer, wherein data analysis layer is mainly to adopt sensing device to be monitored cell state, data transfer layer utilizes radio-frequency technique and radio network technique to complete the transmission of image data, transfer of data is to data analysis layer, data are processed and data are offered to information service layer by server, for it provides information inquiry.
1, data collection layer
As shown in Figure 2,3, data collection layer of the present invention comprises a plurality of ultra-low-temperature sensor devices 100, described ultra-low-temperature sensor device 100 comprises: Profound hypothermia temperature transducer 101, the temperature data control device 108 be connected with described Profound hypothermia temperature transducer 101 and the transmitting antenna 107 that is arranged on temperature data control device 108 outsides.
Described temperature data control device 108 comprises the housing that forms its outward appearance and is arranged on the mainboard 102 of its enclosure interior and can gives the battery of described mainboard 102 energy supplies.Described mainboard 102 comprises the single-chip microcomputer 103 with Profound hypothermia temperature compensating unit, as the C8051F410 type; Flash chip 105, in program control, single-chip microcomputer 103 being processed to the temperature data obtained writes and is stored in flash chip 105 so that as a part of built-in database of record queries afterwards, described flash chip is selected a lot, as 16 megabit SPI serial flash chip SST25VF016B; Can be simultaneously just single-chip microcomputer 103 process the temperature data obtained and be sent to the radio frequency chip 106 of the profound hypothermia hub the following describes by wireless mode, wherein single-chip microcomputer 103 can adopt ADF7020-1BCPZ; And I 2 c chip 104, it provides I for each chip unit 2c reads and writes bus, selectable I 2the C chip type is a lot, as A8563.
Described temperature sensor 101 is connected with single-chip microcomputer 103 data, and preferably, described Profound hypothermia temperature transducer 101 is connected by the monobus communication interface with 102 of mainboards.
But described energy supply battery adopts the battery of recharge, to extend the useful life of battery, can guarantee the miniaturization of whole temperature data control device ease of use and volume simultaneously.
Because the required profound hypothermia temperature-measuring range of cell storage environment is large; Adopt the radio-frequency transmissions of data, reduced the constraint of data wire physics and distance, effectively in receiving range, can dispose arbitrarily ultra-low-temperature sensor device at profound hypothermia refrigerator or profound hypothermia liquid nitrogen container; But and function battery recharge, recharge is more than 500 times, and utilizes special intelligent battery administrative skill, and not power consumption pond electric weight when external power is arranged further extends useful life of rechargeable battery; Measurement data transmits after compensating unit is processed again, and certainty of measurement is high, and measure error is less than or equal to 0.5 ℃; The measurement consistency is high; Real-time, time delay is less than 1S; The thermometric environment is adaptable; It is convenient to safeguard, can repeatedly charge; Install or charge without destroying the cell memory device.That monitoring system for cells under deep hypothermia refrigeration of the present invention can reach fully is contactless under cell profound hypothermia storage condition, high-precision temperature is measured, installation and maintenance purpose easily, has great Practical significance and social effect.
2, data transfer layer
Data transfer layer of the present invention comprises a plurality of Profound hypothermia temperature hubs 200; each Profound hypothermia temperature transducer 200 is corresponding a plurality of ultra-low-temperature sensor device 100 all; as shown in Figure 4; described Profound hypothermia temperature hub 200 comprises: form the hub outward appearance and protect the housing of internal structure, be arranged on the antenna of outside and be arranged on mainboard 210 and the mainboard power supply device of enclosure interior.Described mainboard 210 comprises the main control chip 201 as main control unit, S3C2410 type as ARM, in order to receive the temperature sensing device radiofrequency signal and signal to be transferred to the concurrent Receiver Module 202 of multiple spot of main control chip, the concurrent received RF module 202 of described multiple spot can receive the temperature radiofrequency signal that a plurality of temperature sensors send, as maximum can the concurrent reception of ten six temperature radio frequencies ADF7020-1BCPZ, in order to the GPS module 205 of current Profound hypothermia temperature hub position information to be provided, be connected and in real time temperature data and gps data be sent to the mixed-media network modules mixed-media 203 of data analysis layer with described main control chip, described mixed-media network modules mixed-media 203 include the spider lines module as RTL8139 network card chip or wireless-transmission network module as GPRS unit etc., the large capacity memory chip 204 of data storage function is provided to main control chip, as HY5DU561622FTP-5 type 256Mb memory chip.Wherein, described mainboard power supply device is external 12V power supply.
3, data analysis layer
Data analysis layer of the present invention adopts B/S IA frame serverPC, it comprises web server 300, the database server 320 be connected with web server 300 respectively and prior-warning device 310, web server 300 is utilized network receiving ability and the literacy of large server, by 10000 level temperature real-time network collection modules, temperature real-time collecting software module and proprietary large capacity storage and retrieving files system, realize stable storage, retrieval and the reporting system of vast capacity data.
For promoting the user, the simple application ability of complication system the user that reduces the complex information platform are got involved to difficulty, the cell temperature that also simultaneously stores customizable in web server is patrolled and examined rule, this patrols and examines regular idiographic flow is to open to the user to formulate, when reaching early warning or alert if, web server is carried out early warning, warning by prior-warning device 310, carry out early warning, the warning of the forms such as acousto-optic or note by pre-defined rule, promote whole fail safe.
4, information service layer
Information service layer of the present invention comprises a plurality of clients 400, as mentioned above, the present invention adopts the B/S framework, client 400 can adopt various ways access web server, as long as there is the computer that can surf the Net just can use, and the client zero dimension is protected, the system extension of web server is very easy to, the temperature data real-time update is fast, can inquire about real time temperature and historical temperature under the transportation state, can also review the cell information of supply chain that inquiry is received.Simultaneously, in conjunction with GPS service module and wireless network, can realize the monitoring in all directions of omnidistance position, state.The use of B/S framework also can be carried out section processes on client computer, thereby has alleviated greatly the burden of server; And increased interactivity, can carry out part and refresh in real time.
In sum, cell profound hypothermia monitoring system of the present invention adopts cell profound hypothermia radio frequency temperature sensor, a plurality of Profound hypothermia temperature transducers are corresponding with a profound hypothermia hub, utilize radio-frequency technique to realize the transmission of temperature data, can realize that temperature troops accurately, real-time Transmission, this system has and comprises a plurality of profound hypothermia hubs simultaneously, it is equipped with mixed-media network modules mixed-media and GPS module, can utilize network that temperature data and position data are passed to web server, and the storage of the over capacity temperature data of server, analyze, form technology and cell temperature are are regularly patrolled and examined, temperature anomaly alert detecting and switch or SMS alarm technology make client can obtain much information, the real-time monitored cellular environment changes.
By the present invention, can accomplish to measure by contactless, high-precision temperature, remote centre storage, data analysis and long-distance on-line, reaching on the basis of high level of accuracy more, realizing that the real time temperature of over the horizon is measured, monitor, patrol and examine, warning and quality state analysis.Reach the state-of-the-art supervision purpose that data are objective reliably, quality accident can be recalled, the real-time cell temperature of contactless over the horizon is monitored.
The above instrument is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (7)

1. one kind for monitoring the monitoring system for cells under deep hypothermia refrigeration of cellular environment, and it is characterized in that: it comprises data collection layer, data transfer layer, data analysis layer and information service layer, wherein,
Described data collection layer comprises a plurality of ultra-low-temperature sensor devices, described ultra-low-temperature sensor device comprises the Profound hypothermia temperature transducer (101) of direct detection ambient temperature, the temperature data control device (108) that is connected and obtains its temperature data and carry out the data processing with described Profound hypothermia temperature transducer (101), described temperature data control device (108) comprises the housing that forms its outward appearance, be arranged on the mainboard (102) of its enclosure interior and can give the power supply device of described mainboard (102) energy supply, described mainboard (102) comprises single-chip microcomputer (103), the storage flash chip (105) of real time temperature data, the radio frequency chip (106) of radiofrequency signal is provided and provides I for each chip unit to Profound hypothermia temperature hub 2the I of C read-write bus 2c chip (104),
Described data transfer layer comprises a plurality of Profound hypothermia temperature hubs, the corresponding a plurality of Profound hypothermia temperature transducers of each Profound hypothermia temperature hub, described Profound hypothermia temperature hub comprises housing, be arranged on the antenna and the mainboard (210) and the mainboard power supply device that are arranged on enclosure interior of outside, described mainboard (210) comprises main control chip (201), in order to the radiofrequency signal that receives ultra-low-temperature sensor device (100) the Receiver Module (202) that signal is transferred to main control chip (201), in order to the GPS module (205) of the current position of Profound hypothermia temperature hub (200) information to be provided, be connected and send data in real time the mixed-media network modules mixed-media (203) of data analysis layer with described main control chip, the large capacity memory chip (204) of data storage function is provided to Master control chip,
Data collection layer utilizes the profound hypothermia sensing device monitored and provide data to data transfer layer the cellular environment state, data transfer layer utilizes radio-frequency technique and network technology to send data to data analysis layer, by server, data is processed and data are offered to information service layer.
2. monitoring system for cells under deep hypothermia refrigeration as claimed in claim 1, it is characterized in that: described single-chip microcomputer has the Profound hypothermia temperature compensating unit.
3. monitoring system for cells under deep hypothermia refrigeration as claimed in claim 2, it is characterized in that: described Profound hypothermia temperature transducer is radio temperature sensor, the temperature data wireless receiving module that described mainboard (102) comprises coupling.
4. monitoring system for cells under deep hypothermia refrigeration as claimed in claim 2 is characterized in that: between described Profound hypothermia temperature transducer (101) and mainboard (102), by the monobus communication interface, be connected.
5. monitoring system for cells under deep hypothermia refrigeration as claimed in claim 4, it is characterized in that: described Receiver Module is the concurrent Receiver Module of wireless multipoint.
6. monitoring system for cells under deep hypothermia refrigeration as claimed in claim 1 is characterized in that: described data analysis layer adopts the B/S framework, and it comprises web server, the database server be connected with web server respectively and prior-warning device.
7. monitoring system for cells under deep hypothermia refrigeration as claimed in claim 6, it is characterized in that: store cell temperature and patrol and examine rule in web server, when reaching early warning or alert if, web server is carried out early warning, warning by prior-warning device.
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CN103823497B (en) * 2014-03-04 2016-02-10 浙江大学宁波理工学院 Low temperature protectant solution process biological tissue's on-line monitoring control device
CN106094777B (en) * 2016-08-26 2018-03-02 北京航空航天大学 A kind of industry internet Hub devices
CN106885641A (en) * 2016-12-31 2017-06-23 新昌县迪斯曼科技有限公司 Monitoring system for cells under deep hypothermia refrigeration
DE102017003171A1 (en) * 2017-03-31 2018-10-04 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. TIEFTEMPERATUR-INDIKATORMISCHUNG, DEVICE AND METHOD FOR MONITORING A TEMPERATURE TRANSITION AT DEEP TEMPERATURES
CN107318827B (en) * 2017-07-03 2020-12-01 上海理工大学 Medical speed-regulating and cooling device with communication function

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