CN102224892A

CN102224892A - Propolis extraction process

Info

Publication number: CN102224892A
Application number: CN2011101179890A
Authority: CN
Inventors: 马器重; 范少丹
Original assignee: DALIAN HAIERSI TECHNOLOGY Co Ltd
Current assignee: DALIAN HAIERSI TECHNOLOGY Co Ltd
Priority date: 2011-05-09
Filing date: 2011-05-09
Publication date: 2011-10-26

Abstract

The invention discloses a propolis extraction process which comprises the following steps: 1) freezing the propolis raw material in a liquid nitrogen environment below -5 DEG C for 10-12 hours, and crushing the propolis into fine powder smaller than 200 meshes for later use; 2) adding edible alcohol of which the weight is 3-4 times of the fine powder into the fine powder, and soaking for 6-8 days while stirring for 20-30 minutes at an interval of 2 hours in the soaking process; 3) performing prefiltration with a 80-100 meshed filter cloth, storing the filtrate in a refrigerator at 0 DEG C for 24 hours, and performing fine filtration with the filter cloth of more than 400 meshes to obtain a filtrate for later use; and 4) performing vacuum concentration under reduced pressure to obtain propolis cream. By adopting the propolis extraction process disclosed by the invention, the propolis purifying rate is high, the loss of volatile oil is little, and the active ingredients are obtained after the extraction; and moreover, the entire purification process has simple steps, saves time and improves the purification efficiency.

Description

A kind of extraction process of propolis

Technical field

The present invention relates to relate to a kind of extraction process of propolis.

Background technology

Propolis is viscous secretion---the mixture of resin and part beeswax, pollen etc. that honeybee comes from bud bud, bark or the stem wound collections of plant.Propolis is the stickum of solid shape, is brown or taupe, and being with of having is dark green, is being with aromatic odor, bitter.The chemist has isolated numerous active materials such as more than 20 kind of chalcopyrite cpds terpenes from propolis and other has the organic compound of BA.Propolis has medical function widely.The propolis external application has the function of antibacterial, anti-inflammatory, pain relieving and dissolving cutin.Therefore it is used to treat skin diseases such as cutaneous tuberculosis, chronic eczema, limitation psoriasis.At surgery, propolis is used for the treatment of corn, burns, bedsore etc.In the department of stomatology, with 2 ~ 4% propolis alcohol leachates as exodontia anesthetic and treatment periodontitis, alveolitis, oral cavity anabrosis etc.In ear-nose-throat department, propolis is used for the treatment of rhinitis, tympanitis, chronic pharyngitis and bradyacusia etc.In internal medicine, propolis is used for the treatment of gastric and duodenal ulcer disease, chronic gastritis, enteritis, pulmonary tuberculosis, nephrophthisis etc.The aerosol of propolis can be treated adult, childrens respiratory tract inflammation, bronchial astehma etc.

Propolis tends to sneak into a small amount of honeybee corpse, wood chip and silt in leaching process, also contain a large amount of beeswaxs and pollen in the propolis raw material, sometimes also may sneak into beavy metal impurity, when utilizing propolis to make medicine, health food and cosmetics for functional component, all must be through the processing of purifying, to remove non-functional component such as impurity, heavy metal and beeswax, pollen etc.

Summary of the invention

In order to overcome above-mentioned defective, the invention provides a kind of extraction process of propolis, operation is simple, and has effectively removed the non-functional component in the propolis raw material.

Technical scheme of the present invention is achieved in that a kind of extraction process of propolis, and step is as follows:

1) with propolis raw material under the liquid nitrogen environment below-5 ℃ freezing 10-12 hour, be ground into the following fine powder of 200 orders then, standby;

2) in fine powder, add the doubly heavy edible alcohol of 3-4, soaked 6-8 days, stirred 20-30 minute every 2 hours in the immersion process;

3) earlier with 80-100 purpose filter cloth coarse filtration, filtrate is carried out the essence filter after 0 ℃ of freezer is stored 24 hours, with the above filter cloth of 400 orders, the filtrate that obtains is stand-by during the essence filter; 4) through the concentrated propolis cream that obtains of vacuum decompression.

Described step 2) concentration of edible alcohol is 70-75% in.

Filter residue in the described step 3) after the coarse filtration carries out the essence filter after adding edible alcohol again.

The vacuum degree control that vacuum decompression concentrates in the described step 4) is under 0.08MPa.

Crushing process in two steps in the described step 1): the piece glue that will freeze earlier is broken into the fritter of 5-10mm, is ground into the fine powder below 200 then in disintegrating machine.

The invention has the beneficial effects as follows: adopt the propolis purifying technique that the present invention relates to, propolis purification rate height, loss of volatile oil is few, and it is many to extract back active ingredient, and the step of whole purifying technique is simple, saves time, the purification efficiency height.

The specific embodiment

The extraction process of 1 one kinds of propolis of embodiment

1) with propolis raw material under the liquid nitrogen environment below-5 ℃ freezing 10 hours, be ground into 200 purpose fine powders then, standby;

2) add the doubly heavy edible alcohol of 3-4 in fine powder, the concentration of alcohol is 70%, soaks 7 days, stirs 20-30 minute every 2 hours in the immersion process;

3) earlier with 80 purpose filter cloth coarse filtration, filtrate is carried out essence filter after 0 ℃ of freezer is stored 24 hours, when essence is filtered with 400 purpose filter clothes, the filtrate that obtains is stand-by, wherein, residual filter residue carries out the essence filter after adding edible alcohol again after the coarse filtration, and the filtrate that obtains also adds wherein stand-by;

4) through the concentrated propolis cream that obtains of vacuum decompression, wherein vacuum degree control is under 0.08MPa, and the alcohol of evaporation utilizes after reclaiming by retracting device once more.

The extraction process of 2 one kinds of propolis of embodiment

1) with propolis raw material under-10 ℃ liquid nitrogen environment freezing 12 hours, pulverize then, the piece glue that will freeze earlier is broken into the fritter of 5-10mm, is ground into 300 purpose fine powders then in disintegrating machine, and is standby;

2) add the doubly heavy edible alcohol of 3-4 in fine powder, the concentration of alcohol is 75%, soaks 7 days, stirs 20-30 minute every 2 hours in the immersion process;

3) earlier with 100 purpose filter cloth coarse filtration, filtrate is carried out essence filter after 0 ℃ of freezer is stored 24 hours, when essence is filtered with 400 purpose filter clothes, the filtrate that obtains is stand-by, wherein, residual filter residue carries out the essence filter after adding edible alcohol again after the coarse filtration, and the filtrate that obtains also adds wherein stand-by;

4) through the concentrated propolis cream that obtains of vacuum decompression, wherein vacuum degree control is below 0.08MPa, and the alcohol of evaporation utilizes after reclaiming by retracting device once more.

The above; only be the preferable specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to replacement or change according to technical scheme of the present invention and inventive concept thereof, all should be encompassed within protection scope of the present invention.

Claims

1. the extraction process of a propolis is characterized in that, processing step is as follows: 1), be ground into the following fine powder of 200 orders then with propolis raw material under the liquid nitrogen environment below-5 ℃ freezing 10-12 hour, and standby; 2) in fine powder, add the doubly heavy edible alcohol of 3-4, soaked 6-8 days, stirred 20-30 minute every 2 hours in the immersion process; 3) earlier with 80-100 purpose filter cloth coarse filtration, filtrate is carried out the essence filter after 0 ℃ of freezer is stored 24 hours, with the above filter cloth of 400 orders, the filtrate that obtains is stand-by during the essence filter; 4) through the concentrated propolis cream that obtains of vacuum decompression.

2. the extraction process of a kind of propolis according to claim 1 is characterized in that, described step 2) in the concentration of edible alcohol be 70-75%.

3. the extraction process of a kind of propolis according to claim 1 and 2 is characterized in that, the filter residue in the described step 3) after the coarse filtration carries out the essence filter after adding edible alcohol again.

4. the extraction process of a kind of propolis according to claim 1 is characterized in that, the vacuum degree control that vacuum decompression concentrates in the described step 4) is under 0.08MPa.

5. the extraction process of a kind of propolis according to claim 1 is characterized in that, crushing process in two steps in the described step 1): the piece glue that will freeze earlier is broken into the fritter of 5-10mm, is ground into the fine powder below 200 then in disintegrating machine.