CN102210763A - Chinese medicinal composition for promoting blood circulation, removing blood stasis, promoting qi circulation and relieving pain and preparation method thereof - Google Patents
Chinese medicinal composition for promoting blood circulation, removing blood stasis, promoting qi circulation and relieving pain and preparation method thereof Download PDFInfo
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a Chinese medicinal composition for promoting blood circulation, removing blood stasis, promoting qi circulation and relieving pain and a preparation method thereof. The Chinese medicinal composition comprises the following components in part by weight: 7.5 to 22.5 parts of hawthorn fruit, 7.5 to 22.5 parts of root of red-rooted salvia, 7.5 to 22.5 parts of kudzuvine root, 1 to 3 parts of notoginseng root and 1 to 3 parts of costustoot. The preparation method for granules comprises the following steps of: crushing notoginseng root and costustoot to form 150 to 250-mesh fine powder by using a micro powder crusher for later use; crushing hawthorn fruit, root of red-rooted salvia and kudzuvine root for form coarse powder; extracting active ingredients from the coarse powder by using supercritical carbon dioxide fluid, after extracting, reducing system pressure to normal pressure, and precipitating and separating extracted substances to obtain extractum; and drying the extractum, crushing the extractum to form 150 to 250-mesh fine powder, mixing with the fine powder of notoginseng root and costustoot and proper amount of dextrin and stevioside uniformly to prepare granules, and packaging to obtain the Chinese medicinal composition. The preparation method has a simple production process, and is high in extraction speed, the active ingredients are easy to separate, the Chinese medicinal composition does not have residual toxicity, and is high in purity and good in curative effect, and structures and properties of active substances are not damaged.
Description
Technical field
The present invention relates to a kind of blood circulation promoting and blood stasis dispelling, promoting the circulation of QI to relieve pain Chinese medicine composition and preparation method thereof.
Background technology
" Chinese medicine ministry standard " the 15th records a kind of XINKESHU JIAONANG, and prescription is Fructus Crataegi, Radix Puerariae, Radix Salviae Miltiorrhizae, Radix Notoginseng, the Radix Aucklandiae.Method for making: the above five tastes, get Radix Notoginseng, Radix Aucklandiae powder is broken into fine powder; Fructus Crataegi, Radix Puerariae added 60% ethanol warm macerating after 30 minutes, the heating and refluxing extraction secondary, and 2.5 hours for the first time, 2 hours for the second time, merge alcohol extract, decompression recycling ethanol is concentrated into relative density 1.35 (20 ℃); Radix Salviae Miltiorrhizae decocts with water secondary, and 2 hours for the first time, 1.5 hours for the second time, collecting decoction filtered, and is concentrated into relative density 1.35 (20 ℃); Above-mentioned two kinds of thick pastes are dry under 80 ℃, be ground into fine powder and Radix Notoginseng, Radix Aucklandiae fine powder mixing, make granule, drying is distributed into 1000, promptly.
As from the foregoing, existing preparation technology adopts alcohol extracting method or water extraction to Fructus Crataegi, Radix Puerariae, Radix Salviae Miltiorrhizae.Existing literature research data shows: the chemical constituent of Fructus Crataegi is organic acid, triterpenes, flavonoid and polysaccharide etc., and flavone and polysaccharide have blood pressure lowering, hypolipemic function preferably.Puerarin has another name called puerarin, is a kind of flavonoid glycoside that extracts from the leguminous plant Radix Puerariae.Puerarin has arrhythmia, coronary artery dilator, protection myocardial ultrastructure, cerebral blood flow increasing amount, anticoagulant, effects such as antioxidation, enhancing human body immunity power and the formation of inhibition human skin melanin.Radix Salviae Miltiorrhizae is the most frequently used Chinese medicine of promoting blood circulation to restore menstrual flow, and known active component is for being the tanshinone liposoluble substance of representative with the tanshinone IIA and being the water-soluble substances of representative with salvianolic acid B, protocatechualdehyde that character to each other differs comparatively great disparity.
Studies show that adopt conventional water extraction, alcohol extracting method to extract the effective ingredient of Chinese crude drug, effect is undesirable, paste-forming rate is big, and is consuming time longer, and extraction efficiency is not high, the separation and purification difficulty, and effective ingredient purity is low.
Summary of the invention
The technical problem to be solved in the present invention is, provides a kind of production technology simple and easy, the product purity height, and the product non-residual toxicity does not damage blood circulation promoting and blood stasis dispelling promoting the circulation of QI to relieve pain Chinese medicine composition of active substance structure and character and preparation method thereof.
The technical scheme that the present invention solves its technical problem employing is:
The present invention's blood circulation promoting and blood stasis dispelling promoting the circulation of QI to relieve pain Chinese medicine composition is made by following parts by weight of Chinese traditional medicine crude drug and adjuvant: Fructus Crataegi 7.5-22.5 part, Radix Salviae Miltiorrhizae 7.5-22.5 part, Radix Puerariae 7.5-22.5 part, Radix Notoginseng 1-3 part, Radix Aucklandiae 1-3 part, stevioside 0.25-0.75 part, dextrin 7.5-22.5 part.
Preferred weight proportion is: 15 parts of Fructus Crataegis, 15 parts of Radix Salviae Miltiorrhizaes, 15 parts of Radix Puerariaes, 2 parts of Radix Notoginseng, 2 parts of the Radix Aucklandiae, 0.5 part of stevioside, 15 parts in dextrin.
The preparation method of the present invention's blood circulation promoting and blood stasis dispelling, promoting the circulation of QI to relieve pain Chinese medicinal composition granules may further comprise the steps: Radix Notoginseng, the Radix Aucklandiae are ground into 150-250 purpose fine powder with the micropowder pulverizer, and standby; With Fructus Crataegi, Radix Salviae Miltiorrhizae, powder of Radix Puerariae is broken into 50-70 order (preferred 60 orders) powder, adopt the supercritical carbon dioxide extraction method to extract its effective active composition, the extraction process condition is as follows: the preferred 35MPa of extracting pressure 34-36MPa(), extraction temperature 48-52 ℃ (preferred 50 ℃), the 110-130 minute extraction time (preferred 120 minutes), preferred 10.0 MPa of separator pressure 9.0-11.0MPa(), separator temperature 39-41 ℃ (preferred 40 ℃), carbon dioxide flow is the preferred 80L/H of 78-82L/H(), extraction finishes, and system pressure is reduced to normal pressure, extract is separated out separation, get extractum,, be ground into 150-250 order fine powder then with extract dry, with Radix Notoginseng, Radix Aucklandiae fine powder and dextrin, the stevioside mixing, make granule, packing.
Added the stevioside of tool hypotensive activity in the medicament composing prescription of the present invention, promptly improved the mouthfeel of preparation, be adapted to middle-older patient especially and take, but Synergistic treatment " three-hypers " symptom again, thus increase the product curative effect.Preparation technology of the present invention adopts the supercritical carbon dioxide extraction technology, and it is simple and easy to have extraction process, and rate of extraction is fast, and the effective active composition is easy to separate, the product non-residual toxicity, and the product purity height does not damage advantages such as the structure of active substance and character.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
Present embodiment composition weight proportioning: 15 parts of Fructus Crataegis, 15 parts of Radix Salviae Miltiorrhizaes, 15 parts of Radix Puerariaes, 2 parts of Radix Notoginseng, 2 parts of the Radix Aucklandiae, 15 parts in dextrin, 0.5 part of stevioside.
Preparation: get Radix Notoginseng, the Radix Aucklandiae and be ground into the 150-250 order with the micropowder pulverizer to get fine powder standby; Fructus Crataegi, Radix Salviae Miltiorrhizae, powder of Radix Puerariae are broken into 60 order powder, adopt its effective active composition of supercritical carbon dioxide extraction (extracting pressure 35MPa, 50 ℃ of extraction temperature, extraction time 2H, separator pressure 10MPa, 40 ℃ of separator temperatures, carbon dioxide flow are 80L/H), extraction finishes, system pressure is reduced to normal pressure, extract is separated out, and gets extractum, then with extract dry, again extract powder is broken into 150-250 order fine powder, with Radix Notoginseng, Radix Aucklandiae fine powder and an amount of dextrin, stevioside mixing, make granule, packing is promptly.
The method of inspection:
1 bag of this product is got in [discriminating] (1), porphyrize, and the 30ml that adds diethyl ether, supersound process (power 300W, frequency 25KHz) 15 minutes filters, and filtrate volatilizes, and residue adds methanol 1ml makes dissolving, as need testing solution.Other gets Radix Aucklandiae control medicinal material 1g, shines medical material solution in pairs with legal system.Get the ursolic acid reference substance again, add dehydrated alcohol and make the solution that every 1ml contains 1mg, in contrast product solution.Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2010 B), draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, with cyclohexane extraction-chloroform-ethyl acetate-formic acid (20: 5: 8: 0.1) be developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear that hot blast blows to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show corresponding aubergine speckle; With the corresponding position of control medicinal material chromatograph on, show identical redness and blue two speckles.
(2) get 1 bag of this product, porphyrize adds methanol 20ml, and supersound process (power 300W, frequency 25KHz) 15 minutes filters, and filtrate is as need testing solution.Other gets the protocatechualdehyde reference substance, adds methanol and makes the solution that every 1ml contains 0.5mg, in contrast product solution.Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2010 B), draw need testing solution 20 μ l, reference substance solution 5 μ l, put respectively on same silica gel g thin-layer plate, with toluene-ethyl acetate-formic acid (8: 5: 0.5) is developing solvent, launches, and takes out, dry, spray is with the mixed solution of 1% phloroglucinol ethanol liquid-sulphuric acid (1: 1).In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
(3) get 1 bag of this product, porphyrize takes by weighing 0.5g, the 10ml that adds diethyl ether, supersound process (power 300W, frequency 25KHz) 5 minutes, filter, discard ether solution, residue volatilizes solvent, add methanol 25ml, reflux 15min is put cold, filter, filtrate evaporate to dryness, residue add water 25ml, and slight fever makes dissolving, with the saturated n-butyl alcohol liquid 25ml of ammonia solution, jolting is extracted, and gets n-butanol extracting liquid, and the ammonia solution saturated with n-butyl alcohol washs 2 times, each 25ml, evaporate to dryness butanol solution, residue add methanol 1ml makes dissolving, as need testing solution.Other gets Radix Notoginseng control medicinal material 0.1g, adds diethyl ether and shines medical material solution in pairs with legal system.Get ginsenoside Rg1's reference substance again, ginsenoside Rb1's reference substance and arasaponin R1 reference substance add methanol and make the mixed solution that every 1ml contains 0.5mg, in contrast product solution.Test according to thin layer chromatography (Chinese Pharmacopoeia version appendix in 2010 VI B), draw each 5 μ l of above-mentioned need testing solution, control medicinal material solution and reference substance solution, put respectively on same silica gel g thin-layer plate, lower floor's solution of placing below 10 ℃ with chloroform-methanol-water (13:7:2) is developing solvent, launch, dry, spray is with 10% ethanol solution of sulfuric acid, and 105 ℃ to be heated to speckle colour developing clear.In the test sample chromatograph, with control medicinal material and the corresponding position of reference substance chromatograph on, show the speckle of same color.
[inspection] should meet every regulation relevant under the granule item (an appendix I of Chinese Pharmacopoeia version in 2010 C).
[assay] measured according to high performance liquid chromatography (an appendix VI of Chinese Pharmacopoeia version in 2010 D).
Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; Methanol-water (25:75) is a mobile phase; The detection wavelength is 250nm.Number of theoretical plate calculates by puerarin peak should be not less than 4000.
It is an amount of to the puerarin reference substance of constant weight that drying under reduced pressure is got in the preparation of reference substance solution, adds water and make the solution that every 1ml contains 30 μ g, promptly.
The about 0.1g of this product is got in the preparation of need testing solution, and accurate the title decides, and puts in the 25ml measuring bottle, adds water 20ml supersound process (power 300W, frequency 25KHz) after 20 minutes, cooling, water is settled to scale, filter with microporous filter membrane (0.45 μ m), get subsequent filtrate, promptly.
Accurate respectively reference substance solution and each the 20 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, promptly.
This product contains Radix Puerariae in puerarin for every bag, must not be less than 29.0mg.
Chinese medicine granules of the present invention and former XINKESHU JIAONANG clinical efficacy are relatively
Case is selected: meet diagnostic criteria patient totally 125 examples, the Chinese medicine granules of establishing invention is the treatment group, and former XINKESHU JIAONANG is a matched group.68 examples are organized in treatment, wherein male 58 examples, women 10 examples, 51 years old mean age.Matched group 57 examples, wherein male 50 examples, women 7 examples, 49 years old mean age.
One, treatment hyperlipidemia
1, diagnostic criteria
Being diagnosed as of latest domestic dyslipidemia: the blood fat project [mg/dl(mmol/L)]
(1) rising 〉=240(〉=6.21 T-CHOL TC: OK range＜200(＜5.17), edge rising 200-239(5.17-6.21))
(2) low-density lipoprotein cholesterol LDL-C: rising 〉=160(〉=4.14 OK range＜130 (＜3.36) edge rising 130-159(3.36-4.14))
(3) HDL-C HDL-C: reduction＜40(＜1.03)
(4) triglyceride TG: edge, OK range＜150 (＜1.69) rising 150 ~ 199 (1.69-2.26) rising 〉=200 (〉=2.26)
2, curative effect determinate standard TCH descends 〉=20%, and TG descends 〉=40%, and HDL-C rising 〉=0.26mmol/L is a produce effects; TCH descends and reaches 10-20%, and TG descends and reaches 20-40%, and HDL rising 〉=0.18mmol/L is effective; It is invalid not reaching effective standard, and TCH rises 〉=10%, and TG rises 〉=10%, and HDL-C descends and reaches 0.18mmol/L for worsening.
Two, treatment myocardial ischemia
1, inclusion criteria 24 hr Ambulatory EKG Monitoring have the T ripple, the ST-T changer (the ST section moves down 〉=0.1mV), except merge serious hypertension, diabetes, pulmonary insufficiency and other severe cardiac disorder person.
2, check 24 hr Ambulatory EKG Monitoring after all cases of efficacy assessment standard are taken medicine.(1) produce effects: ischemic ST-T disappears, and clinical symptoms disappears substantially; (2) effective: the ischemic ST-T frequency of occurrences reduce 50% or the ST section go up 〉=O.05mV but the deviant improve but clinical symptoms is most of; (3) invalid: 24 hr Ambulatory EKG Monitoring no changes, clinical symptoms is improved not obvious.
Three, the curative effect to treatment hyperlipidemia and treatment myocardial ischemia compares
Can find out from last table, treatment hyperlipidemia 45 examples, treatment group total effective rate is 88.89%, the matched group total effective rate is 62.22%; Treatment myocardial ischemia 60 examples, treatment group total effective rate is 85.00%, and the matched group total effective rate is 60.00%, and Chinese medicine granules of the present invention has better therapeutic than matched group.
Claims (3)
1. the Chinese medicine composition of a blood circulation promoting and blood stasis dispelling, promoting the circulation of QI to relieve pain, it is characterized in that, make: Fructus Crataegi 7.5-22.5 part, Radix Salviae Miltiorrhizae 7.5-22.5 part, Radix Puerariae 7.5-22.5 part, Radix Notoginseng 1-3 part, Radix Aucklandiae 1-3 part, dextrin 7.5-22.5 part, stevioside 0.25-0.75 part by following parts by weight of Chinese traditional medicine crude drug and adjuvant.
2. the Chinese medicine composition of blood circulation promoting and blood stasis dispelling promoting the circulation of QI to relieve pain according to claim 1, it is characterized in that, make: 15 parts of Fructus Crataegis, 15 parts of Radix Salviae Miltiorrhizaes, 15 parts of Radix Puerariaes, 2 parts of Radix Notoginseng, 2 parts of the Radix Aucklandiae, 15 parts in dextrin, 0.5 part of stevioside by following parts by weight of Chinese traditional medicine crude drug and adjuvant.
3. the process for producing granula of a blood circulation promoting and blood stasis dispelling promoting the circulation of QI to relieve pain Chinese medicine composition as claimed in claim 1 is characterized in that, may further comprise the steps: Radix Notoginseng, the Radix Aucklandiae are ground into 150-250 purpose fine powder with the micropowder pulverizer, and standby; With Fructus Crataegi, Radix Salviae Miltiorrhizae, powder of Radix Puerariae is broken into 50-70 order powder, adopt the supercritical carbon dioxide extraction method to extract its effective active composition, the extraction process condition is as follows: extracting pressure 34-36MPa, extraction temperature 48-52 ℃, extracted separator pressure 9.0-11.0MPa time 110-130 minute, separator temperature 39-41 ℃, carbon dioxide flow is 78-82L/H, and extraction finishes, and system pressure is reduced to normal pressure, extract is separated out separation, get extractum,, again extract powder is broken into 150-250 order fine powder then with extract dry, with Radix Notoginseng, Radix Aucklandiae fine powder and dextrin, the stevioside mixing, make granule, packing.
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| CN1883587A (en) * | 2006-05-19 | 2006-12-27 | 山东沃华医药科技股份有限公司 | Preparation of 'Xin Ke Shu' tablet for treating coronary heart disease |
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| 《中华中医药学刊》 20080531 梁绪国等 心可舒片对血管内皮细胞凋亡及凋亡调控基因的影响 第26卷, 第5期 * |
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