CN102207456B - Sampling and measuring apparatus for microspectrophotometer and application method thereof - Google Patents
Sampling and measuring apparatus for microspectrophotometer and application method thereof Download PDFInfo
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- CN102207456B CN102207456B CN 201110066094 CN201110066094A CN102207456B CN 102207456 B CN102207456 B CN 102207456B CN 201110066094 CN201110066094 CN 201110066094 CN 201110066094 A CN201110066094 A CN 201110066094A CN 102207456 B CN102207456 B CN 102207456B
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Abstract
The invention discloses a sampling and measuring apparatus for microspectrophotometer, and belongs to the field of microspectrophotometer. The apparatus comprises a distance adjustable upper measuring table an a lower measuring table; the upper measuring table is equipped with a projection fiber seat or a reception fiber seat, and the lower measuring table is equipped with a projection fiber seat, wherein the projection fiber seat and the reception fiber seat are aligned in a line and are equipped with fibers therein; a first jackscrew is arranged on the upper measuring table, and an elevating seat capable of moving up-and-down is arranged on a position, which is corresponding to the first jackscrew, on the lower measuring table, and an electromagnet magnetic cylinder covering the elevating seat is fixed with the lower measuring table. The sampling and measuring apparatus also comprises a detection unit connected with the reception fiber seat, wherein the detection unit includes a photoelectric conversion module, a data reception module, a detection calculation module, a numerical correction module, a light path length selection module and a detection data output module. The numerical correction module is added in the invention to correct a light path length, so that an ordinary user can realize accurate measurement without adjusting the light path length accurately.
Description
Technical field
The invention belongs to the spectrophotometer field, relate in particular to a kind of micro-spectrophotometer.
Background technology
Spectrophotometric method is one of biochemical test method of commonly using, is widely used in the Quantitative detection of the samples such as sugar, nucleic acid, enzyme or albumen; The testing tool of spectrophotometric method is spectrophotometer, the container of placing test sample in the legacy equipment is cuvette, but the inner capacities of cuvette is larger, so when treating the sample product and carrying out spectrophotomelric assay, need to expend relatively large sample to be checked on the one hand, cause the waste of the samples such as precious nucleic acid, albumen, on the other hand when carrying out the detection of different samples to be checked, also need repeatedly to clean cuvette, bring a lot of additional workloads to experimental work.
The appearance of micro-spectrophotometer has solved above-mentioned technical matters effectively, when using micro-spectrophotometer that sample is detected, it mainly utilizes the tension force traction of micro liquid to form light-path, so only need very micro-sample to be checked namely can obtain to detect accurately data, thereby can substitute cuvette fully, have novelty and practicality.Since first micro-spectrophotometer emerges, be subject to unanimously pursuing and favorable comment of global Molecular Biology Lab.
In the prior art, the general mode that adopts as shown in Figure 6 of the liquid sampling of micro-spectrophotometer, opposed and have between the upper measuring table (30) of certain interval and the lower measuring table (32) bridge joint and testing sample (31) up and down, because testing sample has certain surface tension, so the testing sample of bridge joint can not plan a successor, be arranged on like this measurement light that the projection fibre of measuring table penetrates and pass the testing liquid sample, and enter measuring mechanism after being received by the reception optical fiber that is arranged on lower measuring table and measure.
US Patent No. 2002154299 discloses a kind of micro-spectrophotometer of using liquid surface tension sampling, its sampling mechanism comprises base and the pivot arm that is connected by a central shaft, a projection fibre seat that is fixed in pivot arm, a reception fiber bench that is fixed on the base, the sample bridge joint is between described projection fibre seat and described reception fiber bench.Also be connected with jackscrew on pivot arm, be provided with one with the electromagnet of piston at described base, wherein, the upper surface of described piston contacts with the bottom surface of jackscrew.During work, the described micro-spectrophotometer of this technology is regulated the optical path length of micro-spectrophotometer by the electric break-make of control electromagnet, when the pivot arm that namely overturns makes the projection fibre seat relative with the reception fiber bench, adjusting optical path length by moving up and down of adjusting jackscrew is 1.0mm, at this moment, connects circuit described electromagnet is switched on, the electromagnet energising is rear under its magneticaction, jackscrew is subject to downward suction, descends thereby promote described piston, and then optical path length is adjusted to 0.2mm.Conversion between two optical path lengths of this micro-spectrophotometer is to control by the accurate distance that piston moves up and down, usually before dispatching from the factory through strict demarcation, dispatching from the factory generally will no longer change afterwards.
When utilizing above-mentioned micro-spectrophotometer of the prior art that difference sample to be checked is measured, in order to obtain accurately contrast effect, the mensuration of then need under identical optical path length, comparing.And when practical application, after repeatedly taking a sample, distance between the measuring table can be because the long-term use wearing and tearing of the continuous open and close of pivot arm and jackscrew produce micro-variation up and down, that is to say that photometric optical path length changes, this moment is for guaranteeing that measurement result accurately need to readjust, distance between the lower measuring table, and since up and down the distance between the measuring table at most also only have distance about 1mm, utilizing manual technique to carry out the trace adjusting not only needs to require considerable time and effort, and fully not guarantee of degree of accuracy, so just affect inevitably the accuracy of experimental result.If still guarantee detection accuracy after long-term the use, the user just has to seek the recovery that producer carries out Default Value.
Summary of the invention
Technical matters to be solved by this invention is that micro-spectrophotometer of the prior art is because long-term the use, be easy to cause the trace of optical path length when measuring to change, utilize manual adjustments to be difficult to fully this trace be changed proofread and correct, if just have to carry out the recovery of Default Value for the accuracy that guarantees experimental result, but and then provide a kind of micro-spectrophotometer that optical path length is proofreaied and correct that self carries out.
For achieving the above object, the sampling and measuring mechanism of micro-spectrophotometer provided by the invention comprises lower measuring table and the upper measuring table that links together by central shaft, and described upper measuring table can be along described central shaft rotating opening; Measuring table is fixedly installed the projection fibre seat on described, is provided with the reception fiber bench at described lower measuring table, and described projection fibre seat and described reception fiber bench are connected with optical fiber respectively; When described upper measuring table was closed, described projection fibre seat and described reception fiber bench aligned up and down, formed the sample cell that holds sample to be checked; On described on the measuring table, be provided with the first jackscrew perpendicular to vertical perforation of described upper measuring table, position corresponding with described the first jackscrew on described lower measuring table is vertically arranged with the elevating bracket that can longitudinally move up and down, and is set in the outer magnetic cylinder of described elevating bracket; When described upper measuring table was closed, the bottom of described the first jackscrew and the top of described elevating bracket contacted;
Described sampling and measuring device also comprises the detecting unit that is connected with described reception fiber bench, and described detecting unit comprises photoelectric conversion module, data reception module, detection computations module, data calibration module, optical path length selection module and detects data outputting module; Described photoelectric conversion module is connected by grating with described reception fiber bench, and described photoelectric conversion module is connected with described data reception module, and the electric signal after opto-electronic conversion is inputed to described data reception module; Described data reception module also respectively with described detection computations module, data calibration module and optical path length select module to be connected, described detection computations module receives the detection data of described data reception module output and it is calculated, described data calibration module is connected with described detection computations module, described data calibration module receives the result of calculation of described detection computations module output and to this result definite correction coefficient of comparing, and this correction coefficient inputed to described data reception module, the detection computations module is carried out light absorption value according to the correction coefficient of determining and is calculated; Described optical path length selects module to be connected with described detection computations module, described optical path length selects module to receive the result of calculation of described detection computations module output and to this result definite optical path length of comparing, and the optical path length of selecting inputed to described data reception module, the detection computations module is calculated according to corresponding described optical path length again; The detection numerical value output that described detection data outputting module calculates described detection computations module.
On described on the measuring table, also vertically connect perpendicular to described upper measuring table and to be provided with the second jackscrew, with respect to the end of described upper measuring table towards described lower measuring table, the bottom of described the first jackscrew is lower than the bottom of described the second jackscrew.
Be provided with the biasing force parts that can apply towards described elevating bracket biasing force in the bottom of described elevating bracket, the bottom of the other end of described biasing force parts and described magnetic cylinder interior is inconsistent.
Described magnetic cylinder gets when electric, obtains minimum optical path length; During described magnetic cylinder dead electricity, obtain maximum optical path length; Described minimum optical path length is 0.2mm, and maximum optical path length is 1.0mm.
When measuring table is closed on described, groove is offered respectively in the corresponding position on two planes that measuring table is relative with described lower measuring table on described, place magnet in described inside grooves, realize locking to described upper measuring table and described lower measuring table by the magnetic force of described magnet.
Described detecting unit also comprises optical path length selection module, is used for judging that the concentration of sample to be checked is to determine the optical path length of sample to be checked.
Described photoelectric conversion module is photodetector.
The present invention provides a kind of using method of above-mentioned sampling and measuring device for micro-spectrophotometer simultaneously, and it may further comprise the steps:
(1) overturns and close described upper measuring table, itself and described lower measuring table are aligned up and down, blank sample is measured, measure incident intensity I
0
(2) overturn and open described upper measuring table, microstandard solution is added drop-wise on the upper surface of reception fiber bench on the described lower measuring table or projection fibre seat, close described upper measuring table itself and lower measuring table are aligned up and down, tested standard solution bridges between described projection fibre seat and the described reception fiber bench;
(3) by regulating described the first jackscrew, make distance between described projection fibre seat and the described reception fiber bench near maximum optical path length; Described magnetic cylinder is switched on, and under the magneticaction of described magnetic cylinder, described the first jackscrew presses down described elevating bracket, when reaching balance, so that the distance between described projection fibre seat and the described reception fiber bench is near minimum optical path length;
(4) above-mentioned distance adjustment complete after, the described reception fiber bench of described grating pair inner fiber receives synthetic light signal and carries out light splitting, the different wave length signal is divided into monochromatic light, described photoelectric conversion module receives described monochromatic light and respectively its light intensity value I is converted into electric signal, described data reception module receives above-said current signal and described electric signal is processed, and the signal after the described processing transferred to described detection computations module, sample light intensity value I and blank light intensity value I after described detection computations module is according to conversion
0Obtain light absorption value A=-lg (I/I
0), at last the light absorption value that calculates is inputed to described data calibration module, in described data calibration module, described data calibration module is called the standard light absorption value A of standard solution
bCompare, obtain correction coefficient X=A
b/ A, and after depositing correction coefficient in data reception module, proofread and correct and finish;
(5) open described upper measuring table, described projection fibre seat and described reception fiber bench are cleaned, after drying, cleaning gets on the upper surface that micro-sample drop to be checked is added to reception fiber bench on the described lower measuring table or projection fibre seat, close described upper measuring table itself and lower measuring table are aligned up and down, sample to be checked is bridged between described projection fibre seat and the described reception fiber bench;
(6) the magnetic cylinder is got electric, obtain minimum optical path length, optical fiber in the described reception fiber bench with optical signal transmission to described grating, the monochromatic light signal that synthetic light signal is divided into different wave length, described photoelectric conversion module receives described monochromatic light and respectively its light intensity value I is converted into electric signal, described data reception module receives above-said current signal and described electric signal is processed, and the signal after the described processing transferred to described detection computations module, call the correction coefficient X of the minimum optical path length in the described data reception module in described detection computations module, and the signal after the described processing is calculated light absorption value A=-lg (I/I
0) * X, and concentration value C=A
0/ El is after the light absorption value A that records is sent to described optical path length and selects module, with the absorbance threshold value A
YCompare;
(7) record light absorption value A greater than described absorbance threshold value A
YThe time, light absorption value is measured in described detection data outputting module output and concentration value is shown to the user; Record light absorption value less than described absorbance threshold value A
YThe time, magnetic cylinder dead electricity, obtain maximum optical path length after, through behind the photoelectric conversion module, data reception module, detection computations module, data outputting module will be measured light absorption value and concentration value is shown to the user through detecting again.
In described step (2), close after described upper measuring table aligns up and down itself and lower measuring table, utilize magnet that described upper measuring table and described lower measuring table are locked.
The described maximum optical path length of above-mentioned steps (3) is 1mm, and minimum optical path length is 0.2mm.
In the step (3), in the situation that described magnetic cylinder gets is electric, described upper measuring table moves down, and by regulating described the second jackscrew, makes distance between described projection fibre seat and the described reception fiber bench near minimum optical path length.
The present invention has following advantage:
The present invention has added the sampling and measuring device with the data calibration module, after obtaining different optical path length correction coefficient by the data calibration module, treat and add this correction coefficient when the sample product are measured, realize the automatic calibration of micro-spectrophotometer measured value, improved the accuracy that micro-spectrophotometer is measured.Whole process realizes automatically by modules, and the common user who uses this micro-spectrophotometer is conveniently regulated.Do not have correction link with micro-spectrophotometer of the prior art, compare by dispatching from the factory accurately to set, made things convenient for user's use, guaranteed simultaneously measuring accuracy.
In addition, be provided with on the upper measuring table of the sampling and measuring device of this micro-spectrophotometer two jackscrews by electromagnet break-make and regulate two jackscrews and stretch out two different optical path lengths of length adjustment of upper measuring table lower end; Two different optical path lengths are regulated by the break-make of a jackscrew and electromagnet from the micro-spectrophotometer of documents and to be compared, greatly reduce the lifting accuracy requirement of electromagnet, reduced manufacturing cost.Simultaneously, this sampling and measuring device selects 0.2mm and two kinds of optical path lengths of 1.0mm to measure, select 1mm for the sample optical path length to be checked that concentration is large, the sample optical path length to be checked that concentration is little is selected 0.2mm, guarantees the accuracy of the measured value of the samples such as sugar, nucleic acid, enzyme or albumen that concentration value differs greatly.
Description of drawings
For content of the present invention is more likely to be clearly understood, the below according to a particular embodiment of the invention and by reference to the accompanying drawings, the present invention is further detailed explanation, wherein
Fig. 1 is the sampling and measuring device structural representation for micro-spectrophotometer of the present invention;
Fig. 2 is the sampling and measuring device structural representation that comprises two jackscrews;
Fig. 3 is the structured flowchart of detecting unit of the present invention;
Fig. 4-1 is the working state figure under the maximum optical path length in the situation of a jackscrew of the present invention;
Fig. 4-2 is the working state figure under the minimum optical path length in the situation of a jackscrew of the present invention;
Fig. 5-1 is the working state figure under the maximum optical path length in the situation of two jackscrews of the present invention;
Fig. 5-2 is the working state figure under the minimum optical path length in the situation of two jackscrews of the present invention;
Fig. 6 is the keeping method key diagram of trace liquid sample;
Fig. 7 is correcting process figure of the present invention;
Fig. 8 is measurement procedure figure of the present invention.
Reference numeral is expressed as among the figure:
Measuring table under the upper measuring table 5-of 1-the first jackscrew 2-the second jackscrew 3-projection fibre seat 4-
6-receives the ferromagnetic cylinder 10-of fiber bench 7-elevating bracket 8-spring 9-electromagnetism magnet 11-central shaft
20-detects measuring table under the upper measuring table 31-sample 32-of the 30-of unit.
Embodiment
Below with reference to accompanying drawing, use following examples that the present invention is further set forth.
Figure 1 shows that the structural representation of sampling and measuring device of the present invention, comprise the upper measuring table 4 and the lower measuring table 5 that are connected by central shaft 11, described upper measuring table 4 can be along described central shaft 11 rotating opening; A projection fibre seat 3 that is fixed in measuring table 4, a reception fiber bench 6 that is fixed in lower measuring table 5, projection fibre seat 3 is inserted with optical fiber with receiving fiber bench 6 inside, during described upper measuring table 4 closure, parallel with lower measuring table 5 at upper measuring table 4, align up and down; Between projection fibre seat 3 and reception fiber bench 6, form the sample cell that holds sample to be checked.
On described on the measuring table 4, be provided with the first jackscrew 1 perpendicular to described upper measuring table 4 vertical perforations, position corresponding with described the first jackscrew 1 on described lower measuring table 5 is vertically arranged with the elevating bracket 7 that can longitudinally move up and down, and is set in the outer ferromagnetic cylinder 9 of electromagnetism of described elevating bracket 7; During described upper measuring table 4 closure, the top of the bottom of described the first jackscrew 1 and described elevating bracket 7 contacts.
Between the ferromagnetic cylinder 9 of described electromagnetism and the elevating bracket 7 spring 8 is arranged, by spring 8 elevating bracket 7 is not withstood on the position concordant with lower measuring table 5 upper surfaces when the ferromagnetic cylinder 9 of electromagnetism is switched on, the ferromagnetic cylinder 9 of electromagnetism is inhaled elevating bracket 7 lower after the energising.As preferred embodiment a kind of, upper measuring table 4 of the present invention and lower measuring table 5 can also be respectively equipped with a groove, the inner magnet 10 of placing, at upper measuring table 4 during with lower measuring table 5 parallel placement, two magnet align up and down, under in working order upper measuring table 4 and lower measuring table 5 are locked.
Sampling and measuring device of the present invention also comprises the detecting unit 20 that is connected with described reception fiber bench 6, as shown in Figure 3.Described detecting unit 20 comprises photoelectric conversion module, data reception module, detection computations module, data calibration module, optical path length selection module and detects data outputting module;
The described photoelectric conversion module of the present embodiment is photodetector, described photodetector and described reception fiber bench 6 are connected by grating, described grating is by receiving the synthetic light signal of fiber bench inner fiber transmission, the different wave length signal is divided into monochromatic light, photodetector exports described data reception module to after receiving respectively monochromatic light and respectively its light intensity value being converted into electric signal;
Described data reception module also respectively with described detection computations module, the data calibration module and, optical path length selects module to be connected, described detection computations module receives incident intensity value and the output intensity value of described data reception module output it is carried out light absorption value calculating, described data calibration module is connected with described detection computations module, described data calibration module receives the result of calculation of described detection computations module output and to this result and standard light absorption value definite correction coefficient of comparing, and store after this correction coefficient inputed to described data reception module, when measuring solution to be measured, the correction coefficient of storage re-starts calculating to light absorption value in the detection computations module calling data receiver module; Described optical path length selects module to be connected with described detection computations module, solution light absorption value result and absorbance threshold ratio that described optical path length selects module to receive described detection computations module output are determined optical path length more afterwards, control the electric and dead electricity that gets of the ferromagnetic cylinder 9 of described electromagnetism, and then select minimum light path or maximum light path, store after simultaneously the optical path length of selecting being inputed to described data reception module, the detection computations module is calculated light absorption value and the concentration that finally draws described sample according to corresponding described optical path length, the detection numerical value output that described detection data outputting module calculates described detection computations module.
Fig. 4-1, Fig. 4-2 is the working state figure of the present invention at two optical path lengths.Fig. 4-1 is the duty of this device when the maximum light path, at first with ferromagnetic cylinder 9 dead electricity of electromagnetism, elevating bracket 7 is after rising to the position concordant with the upper surface of lower measuring table 5 under the effect of spring 8, the distance that changes between time measuring table 5 and the upper measuring table 4 by regulating the first jackscrew 1, and then regulate maximum optical path length; Fig. 4-2 is the duty of this device when the minimum light path, at first get the ferromagnetic cylinder 9 of electromagnetism electric, elevating bracket 7 descends under the magneticaction of the ferromagnetic cylinder 9 of electromagnetism, by the lower measuring table 5 of dropping distance change of the ferromagnetic cylinder 9 of control electromagnetism and the distance between the upper measuring table 4, and then regulate minimum optical path length.
In the present embodiment, maximum optical path length is selected 1mm, and minimum optical path length is selected 0.2mm.
According to langbobier law as can be known, light absorption value
A?=-lg(I/I
0)=?ECl
Wherein the I-sample throws light intensity; I
0-incident intensity (blank projection light intensity);
E-absorptivity (L/mol*cm); C-sample concentration (mol/L); L-optical path length (cm);
Generally, the light absorption value A of standard solution
bThat cuvette by a 1cm optical path length records.Like this, in the situation that photometer light path length of the present invention is 0.2mm or 1.0mm, scale-up factor X of corresponding adding
1=50 or 10 namely realized corresponding with traditional light absorption value.
When application the present invention carries out light absorption value and measurement of concetration, at first to carry out position correction to this sampling and measuring device, after position correction obtains correction coefficient, testing sample accurately be measured.Trimming process of the present invention and measuring process are as shown in Figure 7 and Figure 8.
The correcting process of sampling and measuring of the present invention mechanism is as follows:
At first, measuring table 4 aligns up and down itself and lower measuring table 5 in the upset, and blank sample is measured, and obtains incident intensity I by described photoelectric conversion module
0
Then, measuring table 4 makes 5 one-tenth 90 of itself and lower measuring tables in the upset
0, the 1-2ul standard solution is positioned over the upper surface of accepting fiber bench 6 on the lower measuring table 5 after, measuring table 4 aligns up and down itself and lower measuring table 5 in the upset, makes tested standard solution bridge at projection fibre seat 3 and receives on the fiber bench 6.
If wish to get the correction coefficient of maximum light path 1mm, at first with electromagnet 8 dead electricity, elevating bracket 7 makes projection fibre seat 3 and receives between the fiber bench 6 distance near ultimate range 1mm by the position of regulating the first jackscrew after rising to the position concordant with the upper surface of lower measuring table 5 under the effect of spring; Obtained the projection light intensity I of tested standard solution this moment by described photoelectric conversion module
1, described data reception module receives incident intensity I
0With projection light intensity I
1Signal after transfer to described detection computations module and calculate, the detection computations module by light absorption value A=-lg (I/I
0) while additional proportion coefficient X
0=10 correction light absorption value A that can obtain standard solution this moment
1, described detection computations module will be proofreaied and correct light absorption value A
1Be sent to described data calibration module, described data calibration module and standard light absorption value A
bAfter obtain correction coefficient X
1=A
b/ A
1, and with correction coefficient X
1Deposit in the data reception module, proofread and correct and finish;
If wish to get the correction coefficient of minimum optical path length 0.2mm, at first with electromagnet 8 energising, the displacement of control electromagnet 8 makes projection fibre seat 3 and receives between the fiber bench 6 apart near minor increment 0.2mm; Obtained the projection light intensity I of tested standard solution this moment by described photoelectric conversion module
1, described data reception module receives incident intensity I
0With projection light intensity I
1Signal after transfer to described detection computations module and calculate, the detection computations module by light absorption value A=-lg (I/I
0) while additional proportion coefficient X
0=50 correction light absorption value A that can obtain standard solution this moment
0.2, described detection computations module will be proofreaied and correct light absorption value A
1Be sent to described data calibration module, described data calibration module and standard light absorption value A
bAfter obtain correction coefficient X
0.2=A
b/ A
0.2, and with correction coefficient X
0.2Deposit in the data reception module, proofread and correct and finish.
Measurement procedure of the present invention is as follows:
Open described upper measuring table 4, described projection fibre seat 3 and described reception fiber bench 6 are cleaned, get after the cleaning on the upper surface that micro-sample drop to be checked is added to the reception fiber bench 6 on the described lower measuring table 5, close described upper measuring table 4 itself and lower measuring table 5 are aligned up and down, sample to be checked is bridged between described projection fibre seat 3 and the described reception fiber bench 6; By magnet 10 described upper measuring table 4 is locked with lower measuring table 5.
Because the difference of different solutions concentration, light absorption value is widely different, if when sample concentration is very little, is 1mm when measuring with optical path length, and the projection light intensity I of sample is close to incident intensity I
0, the light absorption value A that obtains=-lg (I/I
0) minimum, can not reach the differentiation effect; When if sample concentration is very high, be 0.2mm when measuring with optical path length, the projection light intensity I of sample is close to 0, light absorption value A=-lg (I/I
0) infinity, can not reach the differentiation effect;
Therefore, select to set in the module absorbance threshold value A of a critical concentration value at described optical path length
Y, this absorbance threshold value A
YChoose according to concrete experiment experience value and decide, the present embodiment is chosen A
Y=1, during measurement, described data reception module is with light intensity value I and the incident intensity I of sample
0After inputing to described detection computations module, after process detection computations module calculates the light absorption value A of sample, be sent to described optical path length and select module.
During actual measurement, the ferromagnetic cylinder 9 of electromagnetism is got electric, optical path length forwards 0.2mm to, when recording sample light absorption value A〉A
YThe time, show that this sample concentration is suitable for this optical path length and measures.This moment is by measuring the light intensity I of blank and sample
0And I
0.2, described detection computations module is called the correction coefficient X that obtains from data reception module
1, calculate the light absorption value A of sample
0.2=-lg (I
0.2/ I
0) * X
0.2, can get sample concentration value C simultaneously
0.2=A
0.2/ El; And with light absorption value A
1With C
1Export described detection data outputting module to, finish and measure and show.
When recording sample light absorption value A＜A
YThe time, show that the applicable optical path length of this sample concentration is that 1mm measures.Described optical path length selection this moment module is sent instruction makes ferromagnetic cylinder 9 dead electricity of electromagnetism, and optical path length forwards 1mm to, by measuring the light intensity I of blank and sample
0And I
1, described detection computations module is called the correction coefficient X that obtains from data reception module
1, calculate the light absorption value A of sample
1=-lg (I
1/ I
0) * X
1, can get sample concentration value C simultaneously
1=A
1/ El; And with light absorption value A
1With C
1Export described detection data outputting module to, finish and measure and show.
Figure 2 shows that the structural representation of the sampling and measuring device that two jackscrews are set, the distinctive points of its structure and embodiment 1 described structure is that this sampling and measuring device also is provided with the second jackscrew 2, concrete setting position is: on described on the measuring table 4, going back vertically perpendicular to described upper measuring table 4, perforation is provided with the second jackscrew 2, with respect to the end of described upper measuring table 4 towards described lower measuring table 5, the bottom of described the first jackscrew 1 is lower than the bottom of described the second jackscrew 2.
Fig. 5-1, Fig. 5-2 are that the sampling and measuring device of the present embodiment is at the working state figure of two optical path lengths.Fig. 5-1 is the duty of this device when the maximum light path, at first with ferromagnetic cylinder 9 dead electricity of electromagnetism, elevating bracket 7 is after rising to the position concordant with the upper surface of lower measuring table 5 under the effect of spring 8, the distance that changes between time measuring table 5 and the upper measuring table 4 by regulating the first jackscrew 1, and then regulate maximum optical path length; Fig. 5-2 is the duty of this device when the minimum light path, at first get the ferromagnetic cylinder 9 of electromagnetism electric, elevating bracket 7 descends under the magneticaction of the ferromagnetic cylinder 9 of electromagnetism, the distance that changes between time measuring table 5 and the upper measuring table 4 by regulating the second jackscrew 2, and then regulate minimum optical path length.
In the present embodiment, maximum optical path length is selected 1mm, and minimum optical path length is selected 0.2mm.
The present embodiment stretches out two different optical path lengths of length adjustment of upper measuring table lower end by regulating two jackscrews, greatly reduce the lifting accuracy requirement of electromagnet, has reduced manufacturing cost.
When application the present embodiment carries out light absorption value and measurement of concetration, comprise equally and proofread and correct and measure two processes.Its flow process as shown in Figure 7 and Figure 8.
The correcting process of the present embodiment is as shown in Figure 7:
At first, measuring table 4 aligns up and down itself and lower measuring table 5 in the upset, and blank sample is measured, and obtains incident intensity I this moment
0
Then, measuring table 4 makes 5 one-tenth 90 of itself and lower measuring tables in the upset
0, the 1-2ul standard solution is positioned over the upper surface of accepting fiber bench 6 on the lower measuring table 5 after, measuring table 4 aligns up and down itself and lower measuring table 5 in the upset, makes tested standard solution bridge at projection fibre seat 3 and receives on the fiber bench 6.
If wish to get the correction coefficient of maximum light path 1mm, at first with electromagnet 8 dead electricity, elevating bracket 7 makes projection fibre seat 3 and receives between the fiber bench 6 distance near ultimate range 1mm by the position of regulating the first jackscrew after rising to the position concordant with the upper surface of lower measuring table 5 under the effect of spring; Obtained the projection light intensity I of tested standard solution this moment by described photoelectric conversion module
1, described data reception module receives incident intensity I
0With projection light intensity I
1Signal after transfer to described detection computations module and calculate, the detection computations module by light absorption value A=-lg (I/I
0) while additional proportion coefficient X
0=10 correction light absorption value A that can obtain standard solution this moment
1, described detection computations module will be proofreaied and correct light absorption value A
1Be sent to described data calibration module, described data calibration module and standard light absorption value A
bAfter obtain correction coefficient X
1=A
b/ A
1, and with correction coefficient X
1Deposit in the data reception module, proofread and correct and finish;
If wish to get the correction coefficient of minimum optical path length 0.2mm, at first with electromagnet 8 energising, elevating bracket 7 inhaled lower after, make projection fibre seat 3 and receive between the fiber bench 6 apart near minor increment 0.2mm by the position of regulating the second jackscrew 2; Obtained the projection light intensity I of tested standard solution this moment by described photoelectric conversion module
1, described data reception module receives incident intensity I
0With projection light intensity I
1Signal after transfer to described detection computations module and calculate, the detection computations module by light absorption value A=-lg (I/I
0) while additional proportion coefficient X
0=50 correction light absorption value A that can obtain standard solution this moment
0.2, described detection computations module will be proofreaied and correct light absorption value A
1Be sent to described data calibration module, described data calibration module and standard light absorption value A
bAfter obtain correction coefficient X
0.2=A
b/ A
0.2, and with correction coefficient X
0.2Deposit in the data reception module, proofread and correct and finish.
The measuring process of the present embodiment is consistent with the measuring process of embodiment 1.
Although the present invention elaborates it by embodiment; but; those skilled in the art should be understood that any form that does not exceed the claim protection domain made on this basis and the variation of details, all belong to invention which is intended to be protected.
Claims (12)
1. sampling and measuring device that is used for micro-spectrophotometer, it comprises
Upper measuring table (4) and lower measuring table (5), the distance between described upper measuring table (4) and the described lower measuring table (5) is adjustable;
Measuring table (4) is provided with projection fibre seat (3) or receives fiber bench (6) on described, be provided with reception fiber bench (6) or projection fibre seat (3) at described lower measuring table (5), described projection fibre seat (3) and described reception fiber bench (6) are connected with optical fiber respectively; Described projection fibre seat (3) and described reception fiber bench (6) align up and down, are used for holding sample to be checked;
On described on the measuring table (4), be provided be used to the first jackscrew (1) of regulating distance between described upper measuring table (4) and the described lower measuring table (5), be provided with the elevating bracket (7) that can move up and down in the upper position corresponding with described the first jackscrew (1) of described lower measuring table (5), and be set in the ferromagnetic cylinder of electromagnetism (9) that described elevating bracket (7) is outer and described lower measuring table (5) is fixing; Described upper measuring table (4) and described lower measuring table (5) are up and down to timing, and the top of the bottom of described the first jackscrew (1) and described elevating bracket (7) contacts;
It is characterized in that:
Described sampling and measuring device also comprises the detecting unit (20) that is connected with described reception fiber bench (6), and described detecting unit (20) comprises photoelectric conversion module, data reception module, detection computations module, data calibration module, optical path length selection module and detects data outputting module;
Described photoelectric conversion module is connected by grating with described reception fiber bench (6), and described photoelectric conversion module is connected with described data reception module, and the electric signal after opto-electronic conversion is inputed to described data reception module;
Described data reception module also respectively with described detection computations module, data calibration module and optical path length select module to be connected, described detection computations module receives the detection data of described data reception module output and it is calculated, described data calibration module is connected with described detection computations module, described data calibration module receives the result of calculation of described detection computations module output and to this result definite correction coefficient of comparing, and this correction coefficient inputed to described data reception module, the detection computations module is carried out light absorption value according to the correction coefficient of determining and is calculated; Described optical path length selects module to be connected with described detection computations module, described optical path length selects module to receive the result of calculation of described detection computations module output and to this result definite optical path length of comparing, and the optical path length of selecting inputed to described data reception module, the detection computations module is calculated according to corresponding described optical path length again; The detection numerical value output that described detection data outputting module calculates described detection computations module.
2. the sampling and measuring device for micro-spectrophotometer according to claim 1 is characterized in that:
On described on the measuring table (4), going back vertically perpendicular to described upper measuring table (4), perforation is provided with the second jackscrew (2), with respect to the end of described upper measuring table (4) towards described lower measuring table (5), the bottom of described the first jackscrew (1) is lower than the bottom of described the second jackscrew (2).
3. the sampling and measuring device for micro-spectrophotometer according to claim 1 and 2 is characterized in that:
Described upper measuring table (4) is connected by central shaft (11) with described lower measuring table (5), and described upper measuring table (4) can be along described central shaft (11) rotating opening.
4. the sampling and measuring device for micro-spectrophotometer according to claim 3 is characterized in that:
Be provided with the biasing force parts (8) that can apply towards described elevating bracket (7) biasing force in the bottom of described elevating bracket (7), the bottom of the other end of described biasing force parts (8) and the ferromagnetic cylinder of described electromagnetism (9) inside is inconsistent.
5. the sampling and measuring device for micro-spectrophotometer according to claim 4 is characterized in that:
The ferromagnetic cylinder of described electromagnetism (9) when electric, obtain minimum optical path length; During the ferromagnetic cylinder of described electromagnetism (9) dead electricity, obtain maximum optical path length.
6. the sampling and measuring device for micro-spectrophotometer according to claim 5 is characterized in that:
Described minimum optical path length is 0.2mm, and maximum optical path length is 1.0mm.
7. it is characterized in that according to claim 1 and 2 or 4 or 5 or 6 described sampling and measuring devices for micro-spectrophotometer:
Measuring table on described (4) is when closing, groove is offered respectively in the corresponding position on two planes that measuring table (4) is relative with described lower measuring table (5) on described, place magnet (10) in described inside grooves, realize locking to described upper measuring table (4) and described lower measuring table (5) by the magnetic force of described magnet (10).
8. it is characterized in that according to claim 1 and 2 or 4 or 5 or 6 described sampling and measuring devices:
Described photoelectric conversion module is photodetector.
9. the using method of the described sampling and measuring device for micro-spectrophotometer of claim 1 may further comprise the steps:
(1). measuring table in the adjusting (4), itself and described lower measuring table (5) are aligned up and down, blank sample is measured, measure incident intensity I
0
(2). microstandard solution is added drop-wise on the upper surface of reception fiber bench (6) on the described lower measuring table (5) or projection fibre seat (3), regulate described upper measuring table (4) itself and lower measuring table (5) are aligned up and down, tested standard solution is bridged between described projection fibre seat (3) and the described reception fiber bench (6);
(3). by regulating described the first jackscrew, make distance between described projection fibre seat (3) and the described reception fiber bench (6) near maximum optical path length; The ferromagnetic cylinder of described electromagnetism (9) is switched on, under the magneticaction of electromagnet, described the first jackscrew (1) presses down described elevating bracket (7), when reaching balance, so that the distance between described projection fibre seat (3) and the described reception fiber bench (6) is near minimum optical path length;
(4). after above-mentioned distance adjustment is complete, the described reception fiber bench of described grating pair inner fiber receives synthetic light signal and carries out light splitting, the different wave length signal is divided into monochromatic light, described photoelectric conversion module receives described monochromatic light and respectively its light intensity value I is converted into electric signal, described data reception module receives above-said current signal and described electric signal is processed, and the signal after the described processing transferred to described detection computations module, sample light intensity value I and blank light intensity value I after described detection computations module is according to conversion
0Obtain light absorption value A=-lg (I/I
0), at last the light absorption value that calculates is inputed to described data calibration module, in described data calibration module, described data calibration module is called the standard light absorption value A of standard solution
bCompare, obtain correction coefficient X=A
b/ A, and after depositing correction coefficient in data reception module, proofread and correct and finish;
(5). described projection fibre seat (3) and described reception fiber bench (6) are cleaned, get after the cleaning on the upper surface that micro-sample drop to be checked is added to reception fiber bench (6) on the described lower measuring table (5) or projection fibre seat (3), regulate described upper measuring table (4) itself and lower measuring table (5) are aligned up and down, sample to be checked is bridged between described projection fibre seat (3) and the described reception fiber bench (6);
(6). the ferromagnetic cylinder of electromagnetism (9) is got electric, obtain minimum optical path length, optical fiber in the described reception fiber bench (6) with optical signal transmission to described grating, the monochromatic light signal that synthetic light signal is divided into different wave length, described photoelectric conversion module receives described monochromatic light and respectively its light intensity value I is converted into electric signal, described data reception module receives above-said current signal and described electric signal is processed, and the signal after the described processing transferred to described detection computations module, call the correction coefficient X of the minimum optical path length in the described data reception module in described detection computations module, and the signal after the described processing is calculated light absorption value A=-lg (I/I
0) * X, and concentration value C=A
0/ El is after the light absorption value A that records is sent to described optical path length and selects module, with the absorbance threshold value A
YCompare;
(7). record light absorption value A greater than described absorbance threshold value A
YThe time, light absorption value is measured in described detection data outputting module output and concentration value is shown to the user; Record light absorption value less than described absorbance threshold value A
YThe time, the ferromagnetic cylinder of electromagnetism (9) dead electricity, obtain maximum optical path length after, through behind the photoelectric conversion module, data reception module, detection computations module, data outputting module will be measured light absorption value and concentration value is shown to the user through detecting again.
10. the using method of the sampling and measuring device for micro-spectrophotometer according to claim 9 is characterized in that:
In described step (2), described upper measuring table (4) utilizes magnet (10) that described upper measuring table (4) and described lower measuring table (5) are locked after aligning up and down with lower measuring table (5).
11. the using method of the sampling and measuring device for micro-spectrophotometer according to claim 9 is characterized in that:
The described maximum optical path length of step (3) is 1mm, and minimum optical path length is 0.2mm.
12. the using method of the sampling and measuring device for micro-spectrophotometer according to claim 9 is characterized in that:
In described step (3), in the situation that the ferromagnetic cylinder of described electromagnetism (9) must be electric, described upper measuring table (3) moves down, and by regulating described the second jackscrew (2), makes distance between described projection fibre seat (3) and the described reception fiber bench (6) near minimum optical path length.
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| CN105547473A (en) * | 2016-01-19 | 2016-05-04 | 济南大学 | Spectrophotometer |
| CN105842178B (en) * | 2016-04-20 | 2018-08-10 | 华侨大学 | A kind of cell culture fluid acid-base value non-intrusion type on-line measuring device and detection method |
| CN106644564B (en) * | 2016-10-12 | 2019-02-05 | 中国地质大学（武汉） | Halmeic deposit gravity column sampling test platform and operating method |
| WO2019012580A1 (en) * | 2017-07-10 | 2019-01-17 | 株式会社島津製作所 | Flame atomic absorption spectrophotometer |
| CN112304878A (en) * | 2019-07-30 | 2021-02-02 | 杭州米欧仪器有限公司 | Spectrophotometer optical fiber head assembly, photometer and method for measuring solution concentration |
| CN113777062A (en) * | 2021-08-24 | 2021-12-10 | 左渡海 | Micro-spectrophotometer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001014855A1 (en) * | 1999-08-20 | 2001-03-01 | Charles William Robertson | Liquid photometer using surface tension to contain sample |
| CN1912585A (en) * | 2005-08-12 | 2007-02-14 | 深圳迈瑞生物医疗电子股份有限公司 | Method and device for automatic correcting and measuring gas concentration using infrared light absorption characteristic |
| CN101180497A (en) * | 2005-02-11 | 2008-05-14 | 纳米微滴技术公司 | Method for measuring fluorescence by surface tension |
| WO2010040104A1 (en) * | 2008-10-03 | 2010-04-08 | Nanodrop Technologies Llc | Optical path length sensor and method for optimal absorbance measurements |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN201955299U (en) * | 2011-03-18 | 2011-08-31 | 赵景琪 | Sampling measurement device for micro-spectrophotometer |
-
2011
- 2011-03-18 CN CN 201110066094 patent/CN102207456B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001014855A1 (en) * | 1999-08-20 | 2001-03-01 | Charles William Robertson | Liquid photometer using surface tension to contain sample |
| CN101180497A (en) * | 2005-02-11 | 2008-05-14 | 纳米微滴技术公司 | Method for measuring fluorescence by surface tension |
| CN1912585A (en) * | 2005-08-12 | 2007-02-14 | 深圳迈瑞生物医疗电子股份有限公司 | Method and device for automatic correcting and measuring gas concentration using infrared light absorption characteristic |
| WO2010040104A1 (en) * | 2008-10-03 | 2010-04-08 | Nanodrop Technologies Llc | Optical path length sensor and method for optimal absorbance measurements |
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