CN102207456A - Sampling and measuring apparatus for microspectrophotometer and application method thereof - Google Patents
Sampling and measuring apparatus for microspectrophotometer and application method thereof Download PDFInfo
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- CN102207456A CN102207456A CN2011100660949A CN201110066094A CN102207456A CN 102207456 A CN102207456 A CN 102207456A CN 2011100660949 A CN2011100660949 A CN 2011100660949A CN 201110066094 A CN201110066094 A CN 201110066094A CN 102207456 A CN102207456 A CN 102207456A
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Abstract
The invention discloses a sampling and measuring apparatus for microspectrophotometer, and belongs to the field of microspectrophotometer. The apparatus comprises a distance adjustable upper measuring table an a lower measuring table; the upper measuring table is equipped with a projection fiber seat or a reception fiber seat, and the lower measuring table is equipped with a projection fiber seat, wherein the projection fiber seat and the reception fiber seat are aligned in a line and are equipped with fibers therein; a first jackscrew is arranged on the upper measuring table, and an elevating seat capable of moving up-and-down is arranged on a position, which is corresponding to the first jackscrew, on the lower measuring table, and an electromagnet magnetic cylinder covering the elevating seat is fixed with the lower measuring table. The sampling and measuring apparatus also comprises a detection unit connected with the reception fiber seat, wherein the detection unit includes a photoelectric conversion module, a data reception module, a detection calculation module, a numerical correction module, a light path length selection module and a detection data output module. The numerical correction module is added in the invention to correct a light path length, so that an ordinary user can realize accurate measurement without adjusting the light path length accurately.
Description
Technical field
The invention belongs to the spectrophotometer field, relate in particular to a kind of micro-spectrophotometer.
Background technology
Spectrophotometric method is one of biochemical test method of using always, and the fast quantification that is widely used in samples such as sugar, nucleic acid, enzyme or albumen detects; The testing tool of spectrophotometric method is a spectrophotometer, the container of placing test sample in the legacy equipment is a cuvette, but the inner capacities of cuvette is bigger, so when treating the sample product and carrying out spectrophotomelric assay, need expend relatively large sample to be checked on the one hand, cause the waste of samples such as precious nucleic acid, albumen, on the other hand when carrying out the detection of different samples to be checked, also need to clean repeatedly cuvette, bring a lot of additional workloads to experimental work.
The appearance of micro-spectrophotometer has solved above-mentioned technical matters effectively, when using micro-spectrophotometer that sample is detected, it mainly utilizes the tension force traction of micro liquid to form light-path, so only need very micro-sample to be checked promptly can obtain to detect accurately data, thereby can substitute cuvette fully, have novelty and practicality.Since first micro-spectrophotometer emerges, be subjected to the consistent of global Molecular Biology Lab and pursued and favorable comment.
In the prior art, the general mode that adopts as shown in Figure 6 of the liquid sampling of micro-spectrophotometer, up and down opposed and have certain interval between measuring table (30) and the following measuring table (32) bridge joint testing sample (31), because testing sample has certain surface tension, so the testing sample of bridge joint can not plan a successor, be arranged on the measuring light that the projection optical fiber of measuring table penetrates like this and pass the testing liquid sample, and enter measuring mechanism after receiving by the reception optical fiber that is arranged on down measuring table and measure.
U.S. Pat 2002154299 discloses a kind of micro-spectrophotometer of using liquid surface tension sampling, its sampling mechanism comprises base and the pivot arm that is connected by a central shaft, a projection fiber bench that is fixed in pivot arm, a reception fiber bench that is fixed on the base, the sample bridge joint is between described projection fiber bench and described reception fiber bench.Also be connected with jackscrew on pivot arm, described base is provided with an electromagnet that has piston, and wherein, the upper surface of described piston contacts with the bottom surface of jackscrew.During work, the described micro-spectrophotometer of this technology is regulated the optical path length of micro-spectrophotometer by the electric break-make of control electromagnet, when the pivot arm that promptly overturns makes the projection fiber bench relative with the reception fiber bench, adjusting optical path length by moving up and down of adjusting jackscrew is 1.0mm, at this moment, connects circuit described electromagnet is switched on, electromagnet energising back is under its magneticaction, jackscrew is subjected to downward suction, descends thereby promote described piston, and then optical path length is adjusted to 0.2mm.Conversion between two optical path lengths of this micro-spectrophotometer is to control by the accurate distance that piston moves up and down, and the strict demarcation of process before dispatching from the factory usually generally will no longer change after dispatching from the factory.
When utilizing above-mentioned micro-spectrophotometer of the prior art that difference sample to be checked is measured, in order to obtain contrast effect accurately, the mensuration of then need under identical optical path length, comparing.And when practical application, after repeatedly taking a sample, distance between the measuring table can be because the long-term use wearing and tearing of the continuous open and close of pivot arm and jackscrew produce micro-variation up and down, that is to say that photometric optical path length changes, this moment is for guaranteeing that measurement result accurately need readjust, distance between the following measuring table, and since up and down the distance between the measuring table at most also have only distance about 1mm, utilizing manual technique to carry out the trace adjusting not only needs to require considerable time and effort, and degree of accuracy not guarantee fully, so just influence the accuracy of experimental result inevitably.If still guarantee detection accuracy after long-term the use, the user just has to seek the recovery that producer carries out Default Value.
Summary of the invention
Technical matters to be solved by this invention is that micro-spectrophotometer of the prior art is because long-term the use, be easy to cause the trace of optical path length when measuring to change, utilize manual adjustments to be difficult to fully this trace be changed proofread and correct, if for the accuracy that guarantees experimental result just has to carry out the recovery of Default Value, but and then provide a kind of micro-spectrophotometer that optical path length is proofreaied and correct that self carries out.
For achieving the above object, the sampling and measuring mechanism of micro-spectrophotometer provided by the invention comprises following measuring table and the last measuring table that links together by central shaft, and described upward measuring table can be along described central shaft rotating opening; Be set with the projection fiber bench on the measuring table on described, described measuring table down is provided with the reception fiber bench, and described projection fiber bench and described reception fiber bench are connected with optical fiber respectively; When described last measuring table was closed, described projection fiber bench and described reception fiber bench aligned up and down, form the sample cell that holds sample to be checked; On described on the measuring table, be provided with first jackscrew perpendicular to vertical perforation of described upward measuring table, on described down measuring table, be vertically arranged with the elevating bracket that can longitudinally move up and down and be set in magnetic cylinder outside the described elevating bracket with the corresponding position of described first jackscrew; When described last measuring table was closed, the bottom of described first jackscrew and the top of described elevating bracket contacted;
Described sampling and measuring device also comprises the detecting unit that is connected with described reception fiber bench, and described detecting unit comprises photoelectric conversion module, data reception module, detection computations module, data calibration module, optical path length selection module and detects data outputting module; Described photoelectric conversion module is connected by grating with described reception fiber bench, and described photoelectric conversion module is connected with described data reception module, and the electric signal after opto-electronic conversion is inputed to described data reception module; Described data reception module also respectively with described detection computations module, data calibration module and optical path length select module to be connected, described detection computations module receives the detection data of described data reception module output and it is calculated, described data calibration module is connected with described detection computations module, described data calibration module receives the result of calculation of described detection computations module output and to this result definite correction coefficient of comparing, and this correction coefficient inputed to described data reception module, the detection computations module is carried out light absorption value according to the correction coefficient of determining and is calculated; Described optical path length selects module to be connected with described detection computations module, described optical path length selects module to receive the result of calculation of described detection computations module output and to this result definite optical path length of comparing, and the optical path length of selecting inputed to described data reception module, the detection computations module is calculated according to corresponding described optical path length again; The detection numerical value output that described detection data outputting module calculates described detection computations module.
On described on the measuring table, also vertically connect and be provided with second jackscrew perpendicular to the described measuring table of going up, towards a described end of measuring table down, the bottom of described first jackscrew is lower than the bottom of described second jackscrew with respect to the described measuring table of going up.
Be provided with the biasing force parts that can apply biasing force towards described elevating bracket in the bottom of described elevating bracket, the bottom of the other end of described biasing force parts and described magnetic cylinder interior is inconsistent.
Described magnetic cylinder gets when electric, obtains minimum optical path length; During described magnetic cylinder dead electricity, obtain maximum optical path length; Described minimum optical path length is 0.2mm, and maximum optical path length is 1.0mm.
When measuring table is closed on described, measuring table is offered groove respectively with the corresponding position on described down two planes that measuring table is relative on described, place magnet in described inside grooves, realize described upward measuring table and the described locking of measuring table down by the magnetic force of described magnet.
Described detecting unit also comprises optical path length selection module, is used to judge that the concentration of sample to be checked is to determine the optical path length of sample to be checked.
Described photoelectric conversion module is a photodetector.
The present invention provides a kind of above-mentioned using method that is used for the sampling and measuring device of micro-spectrophotometer simultaneously, and it may further comprise the steps:
(1) overturns and close the described measuring table of going up, itself and described time measuring table are aligned up and down, blank sample is measured, measure incident intensity I
0
(2) overturn and open the described measuring table of going up, the microstandard drips of solution is added on the upper surface of reception fiber bench on the described down measuring table or projection fiber bench, close the described measuring table of going up itself and following measuring table are aligned up and down, tested standard solution bridges between described projection fiber bench and the described reception fiber bench;
(3), make the approaching maximum optical path length of distance between described projection fiber bench and the described reception fiber bench by regulating described first jackscrew; Described magnetic cylinder is switched on, and under the magneticaction of described magnetic cylinder, described first jackscrew presses down described elevating bracket, when reaching balance, makes distance between described projection fiber bench and the described reception fiber bench near minimum optical path length;
(4) after above-mentioned distance adjustment finishes, the described reception fiber bench of described grating pair inner fiber receives synthetic light signal and carries out beam split, wavelength optical signals is divided into monochromatic light, described photoelectric conversion module receives described monochromatic light and respectively its light intensity value I is converted into electric signal, described data reception module receives above-said current signal and described electric signal is handled, and the signal after the described processing transferred to described detection computations module, sample light intensity value I and blank light intensity value I after described detection computations module is according to conversion
0Obtain light absorption value A=-lg (I/I
0), at last the light absorption value that calculates is inputed to described data calibration module, in described data calibration module, the standard light absorption value A of described data calibration module invokes standard solution
bCompare, obtain correction coefficient X=A
b/ A, and after depositing correction coefficient in data reception module, proofread and correct and finish;
(5) open the described measuring table of going up, described projection fiber bench and described reception fiber bench are cleaned, after drying, cleaning gets on the upper surface that micro-sample drop to be checked is added to reception fiber bench on the described down measuring table or projection fiber bench, close the described measuring table of going up itself and following measuring table are aligned up and down, sample to be checked is bridged between described projection fiber bench and the described reception fiber bench;
(6) the magnetic cylinder is got, obtain minimum optical path length, optical fiber in the described reception fiber bench with optical signal transmission to described grating, the monochromatic light signal that synthetic light signal is divided into different wave length, described photoelectric conversion module receives described monochromatic light and respectively its light intensity value I is converted into electric signal, described data reception module receives above-said current signal and described electric signal is handled, and the signal after the described processing transferred to described detection computations module, the correction coefficient X of the minimum optical path length in the described data reception module of described detection computations module invokes, and the signal after the described processing calculated light absorption value A=-lg (I/I
0) * X, and concentration value C=A
0/ El is sent to the light absorption value A that records after described optical path length selects module, with the absorbance threshold value A
YCompare;
(7) record light absorption value A greater than described absorbance threshold value A
YThe time, light absorption value is measured in described detection data outputting module output and concentration value is shown to the user; Record light absorption value less than described absorbance threshold value A
YThe time, magnetic cylinder dead electricity, obtain maximum optical path length after, through behind the photoelectric conversion module, data reception module, detection computations module, data outputting module will be measured light absorption value and concentration value is shown to the user through detecting again.
In described step (2), close described go up measuring table itself and following measuring table aligned up and down after, utilize magnet will be described on measuring table and described measuring table down lock.
The described maximum optical path length of above-mentioned steps (3) is 1mm, and minimum optical path length is 0.2mm.
In the step (3), get under the electric situation at described magnetic cylinder, the described measuring table of going up moves down, and by regulating described second jackscrew, makes the approaching minimum optical path length of distance between described projection fiber bench and the described reception fiber bench.
The present invention has following advantage:
The present invention has added the sampling and measuring device that has the data calibration module, after obtaining different optical path length correction coefficient by the data calibration module, treat and add this correction coefficient when the sample product are measured, realized the micro-spectrophotometer measured value from normal moveout correction, improved the accuracy that micro-spectrophotometer is measured.Whole process realizes automatically by each module, and the common user who uses this micro-spectrophotometer is conveniently regulated.Do not have correction link with micro-spectrophotometer of the prior art, compare, made things convenient for user's use, guaranteed measuring accuracy simultaneously by dispatching from the factory accurately to set.
In addition, the last measuring table of the sampling and measuring device of this micro-spectrophotometer be provided with two jackscrews by electromagnet break-make and regulate two jackscrews and stretch out two different optical path lengths of length adjustment of measuring table lower end; Two different optical path lengths are regulated by the break-make of a jackscrew and electromagnet with the micro-spectrophotometer of documents and to be compared, greatly reduce the lifting accuracy requirement of electromagnet, reduced manufacturing cost.Simultaneously, this sampling and measuring device selects for use 0.2mm and two kinds of optical path lengths of 1.0mm to measure, select 1mm for use for the sample optical path length to be checked that concentration is big, the sample optical path length to be checked that concentration is little is selected 0.2mm for use, guarantees the accuracy of the measured value of the samples such as sugar, nucleic acid, enzyme or albumen that concentration value differs greatly.
Description of drawings
For the easier quilt of content of the present invention is clearly understood, below according to a particular embodiment of the invention and in conjunction with the accompanying drawings, the present invention is further detailed explanation, wherein
Fig. 1 is the sampling and measuring device structural representation that is used for micro-spectrophotometer of the present invention;
Fig. 2 is the sampling and measuring device structural representation that comprises two jackscrews;
Fig. 3 is the structured flowchart of detecting unit of the present invention;
Fig. 4-1 is the working state figure under the maximum optical path length under the situation of a jackscrew of the present invention;
Fig. 4-2 is the working state figure under the minimum optical path length under the situation of a jackscrew of the present invention;
Fig. 5-1 is the working state figure under the maximum optical path length under the situation of two jackscrews of the present invention;
Fig. 5-2 is the working state figure under the minimum optical path length under the situation of two jackscrews of the present invention;
Fig. 6 is the maintenance method key diagram of trace liquid sample;
Fig. 7 is correcting process figure of the present invention;
Fig. 8 is a measurement process flow diagram of the present invention.
Reference numeral is expressed as among the figure:
The 1-first jackscrew 2-second jackscrew 3-projection fiber bench 4-goes up measuring table under the measuring table 5-
6-receives the ferromagnetic cylinder 10-of fiber bench 7-elevating bracket 8-spring 9-electromagnetism magnet 11-central shaft
20-detects the 30-of unit and goes up measuring table under the measuring table 31-sample 32-.
Embodiment
Below with reference to accompanying drawing, use following examples that the present invention is further set forth.
Figure 1 shows that the structural representation of sampling and measuring device of the present invention, comprise the last measuring table 4 and the following measuring table 5 that are connected by central shaft 11, described upward measuring table 4 can be along described central shaft 11 rotating opening; A projection fiber bench 3 that is fixed in measuring table 4, one is fixed in the down reception fiber bench 6 of measuring table 5, and projection fiber bench 3 is inserted with optical fiber with receiving fiber bench 6 inside, and the described measuring table 4 gone up is when closed, parallel at last measuring table 4 with following measuring table 5, align up and down; Between projection fiber bench 3 and reception fiber bench 6, form the sample cell that holds sample to be checked.
On described on the measuring table 4, be provided with first jackscrew 1 perpendicular to described upward measuring table 4 vertical perforations, on described down measuring table 5, be vertically arranged with the elevating bracket 7 that can longitudinally move up and down and be set in the ferromagnetic cylinder 9 of electromagnetism outside the described elevating bracket 7 with described first jackscrew 1 corresponding position; The described measuring table 4 gone up is when closed, and the top of the bottom of described first jackscrew 1 and described elevating bracket 7 contacts.
Between ferromagnetic cylinder 9 of described electromagnetism and the elevating bracket 7 spring 8 is arranged, by spring 8 elevating bracket 7 is not withstood on and the concordant position of following measuring table 5 upper surfaces when the ferromagnetic cylinder 9 of electromagnetism is switched on, the ferromagnetic cylinder 9 of energising back electromagnetism is inhaled elevating bracket 7 down.As preferred embodiment a kind of, the measuring table 4 of going up of the present invention can also be respectively equipped with a groove with following measuring table 5, the inner magnet 10 of placing, at last measuring table 4 during with following measuring table 5 parallel placements, two magnet align up and down, will go up measuring table 4 under being used in working order and following measuring table 5 locks.
Sampling and measuring device of the present invention also comprises the detecting unit 20 that is connected with described reception fiber bench 6, as shown in Figure 3.Described detecting unit 20 comprises photoelectric conversion module, data reception module, detection computations module, data calibration module, optical path length selection module and detects data outputting module;
The described photoelectric conversion module of present embodiment is a photodetector, described photodetector and described reception fiber bench 6 are connected by grating, described grating is by receiving the synthetic light signal of fiber bench inner fiber transmission, wavelength optical signals is divided into monochromatic light, photodetector exports described data reception module to after receiving monochromatic light respectively and respectively its light intensity value being converted into electric signal;
Described data reception module also respectively with described detection computations module, the data calibration module and, optical path length selects module to be connected, described detection computations module receives the incident intensity value and the output intensity value of described data reception module output it is carried out light absorption value calculating, described data calibration module is connected with described detection computations module, described data calibration module receives the result of calculation of described detection computations module output and to this result and standard light absorption value definite correction coefficient of comparing, and store after this correction coefficient inputed to described data reception module, when measuring solution to be measured, the correction coefficient of detection computations module invokes data reception module stored is calculated again to light absorption value; Described optical path length selects module to be connected with described detection computations module, solution light absorption value result and absorbance threshold ratio that described optical path length selects module to receive described detection computations module output are determined optical path length in the back, control the electric and dead electricity that gets of the ferromagnetic cylinder 9 of described electromagnetism, and then select minimum light path or maximum light path, store after simultaneously the optical path length of selecting being inputed to described data reception module, the detection computations module is calculated light absorption value and the concentration that finally draws described sample according to corresponding described optical path length, the detection numerical value output that described detection data outputting module calculates described detection computations module.
Fig. 4-1, Fig. 4-2 is the working state figure of the present invention at two optical path lengths.Fig. 4-1 is the duty of this device when the maximum light path, at first with ferromagnetic cylinder 9 dead electricity of electromagnetism, elevating bracket 7 is after rising to the position concordant with the upper surface of following measuring table 5 under the effect of spring 8, change the distance between the measuring table 5 and last measuring table 4 down by regulating first jackscrew 1, and then regulate maximum optical path length; Fig. 4-2 is the duty of this device when the minimum light path, at first the ferromagnetic cylinder 9 of electromagnetism is got, elevating bracket 7 descends under the magneticaction of the ferromagnetic cylinder 9 of electromagnetism, by the distance between measuring table 5 and the last measuring table 4 under the dropping distance change of the ferromagnetic cylinder 9 of control electromagnetism, and then regulate minimum optical path length.
In the present embodiment, maximum optical path length is selected 1mm for use, and minimum optical path length is selected 0.2mm for use.
According to langbobier law as can be known, light absorption value
A?=-lg(I/I
0)=?ECl
Wherein the I-sample throws light intensity; I
0-incident intensity (blank projection light intensity);
E-absorptivity (L/mol*cm); C-sample concentration (mol/L); L-optical path length (cm);
Generally, the light absorption value A of standard solution
bBe that cuvette by a 1cm optical path length records.Like this, be under the situation of 0.2mm or 1.0mm in photometer light path length of the present invention, scale-up factor X of corresponding adding
1=50 or 10 promptly realized corresponding with traditional light absorption value.
When application the present invention carries out light absorption value and measurement of concetration, at first to carry out position correction, after position correction obtains correction coefficient, testing sample accurately be measured this sampling and measuring device.Trimming process of the present invention and measuring process are as shown in Figure 7 and Figure 8.
The correcting process of sampling and measuring of the present invention mechanism is as follows:
At first, measuring table 4 aligns itself and following measuring table 5 up and down in the upset, and blank sample is measured, and obtains incident intensity I by described photoelectric conversion module
0
Then, measuring table 4 makes 5 one-tenth 90 of itself and following measuring tables in the upset
0, the 1-2ul standard solution is positioned over down the upper surface of accepting fiber bench 6 on the measuring table 5 after, measuring table 4 aligns itself and following measuring table 5 up and down in the upset, and tested standard solution is bridged on projection fiber bench 3 and the reception fiber bench 6.
If the correction coefficient of wishing to get maximum light path 1mm, at first with electromagnet 8 dead electricity, elevating bracket 7 makes projection fiber bench 3 and receives between the fiber bench 6 apart near ultimate range 1mm by the position of regulating first jackscrew after rising to the position concordant with the upper surface of following measuring table 5 under the effect of spring; Obtain the projection light intensity I of tested standard solution this moment by described photoelectric conversion module
1, described data reception module receives incident intensity I
0With projection light intensity I
1Signal after transfer to described detection computations module and calculate, the detection computations module by light absorption value A=-lg (I/I
0) while additional proportion coefficient X
0=10 correction light absorption value A that can obtain standard solution this moment
1, described detection computations module will be proofreaied and correct light absorption value A
1Be sent to described data calibration module, described data calibration module and standard light absorption value A
bObtain correction coefficient X relatively
1=A
b/ A
1, and with correction coefficient X
1Deposit in the data reception module, proofread and correct and finish;
If the correction coefficient of wishing to get minimum optical path length 0.2mm, at first with electromagnet 8 energisings, the displacement of control electromagnet 8 makes projection fiber bench 3 and receives between the fiber bench 6 apart near minor increment 0.2mm; Obtain the projection light intensity I of tested standard solution this moment by described photoelectric conversion module
1, described data reception module receives incident intensity I
0With projection light intensity I
1Signal after transfer to described detection computations module and calculate, the detection computations module by light absorption value A=-lg (I/I
0) while additional proportion coefficient X
0=50 correction light absorption value A that can obtain standard solution this moment
0.2, described detection computations module will be proofreaied and correct light absorption value A
1Be sent to described data calibration module, described data calibration module and standard light absorption value A
bObtain correction coefficient X relatively
0.2=A
b/ A
0.2, and with correction coefficient X
0.2Deposit in the data reception module, proofread and correct and finish.
Measurement flow process of the present invention is as follows:
Open the described measuring table 4 of going up, described projection fiber bench 3 and described reception fiber bench 6 are cleaned, get after the cleaning on the upper surface that micro-sample drop to be checked is added to the reception fiber bench 6 on the described down measuring table 5, close the described measuring table 4 of going up itself and following measuring table 5 are aligned up and down, sample to be checked is bridged between described projection fiber bench 3 and the described reception fiber bench 6; By magnet 10 the described measuring table 4 of going up is locked with following measuring table 5.
Because the difference of different solutions concentration, light absorption value is widely different, if when sample concentration is very little, is 1mm when measuring with optical path length, and the projection light intensity I of sample approaches incident intensity I
0, the light absorption value A that obtains=-lg (I/I
0) minimum, can not reach the differentiation effect; When if sample concentration is very high, be 0.2mm when measuring with optical path length, the projection light intensity I of sample approaches 0, light absorption value A=-lg (I/I
0) infinity, can not reach the differentiation effect;
Therefore, select to set in the module absorbance threshold value A of a critical concentration value at described optical path length
Y, this absorbance threshold value A
YChoose according to concrete experiment experience value and decide, present embodiment is chosen A
Y=1, during measurement, described data reception module is with the light intensity value I and the incident intensity I of sample
0After inputing to described detection computations module, after process detection computations module calculates the light absorption value A of sample, be sent to described optical path length and select module.
During actual measurement, it is electric that the ferromagnetic cylinder 9 of electromagnetism is got, and optical path length forwards 0.2mm to, when recording sample light absorption value A〉A
YThe time, show that this sample concentration is suitable for this optical path length and measures.This moment is by measuring the light intensity I of blank and sample
0And I
0.2, the correction coefficient X that described detection computations module invokes obtains from data reception module
1, calculate the light absorption value A of sample
0.2=-lg (I
0.2/ I
0) * X
0.2, can get sample concentration value C simultaneously
0.2=A
0.2/ El; And with light absorption value A
1With C
1Export described detection data outputting module to, finish and measure and show.
When recording sample light absorption value A＜A
YThe time, show that it is that 1mm measures that this sample concentration is suitable for optical path length.Described optical path length selection this moment module is sent instruction makes ferromagnetic cylinder 9 dead electricity of electromagnetism, and optical path length forwards 1mm to, by measuring the light intensity I of blank and sample
0And I
1, the correction coefficient X that described detection computations module invokes obtains from data reception module
1, calculate the light absorption value A of sample
1=-lg (I
1/ I
0) * X
1, can get sample concentration value C simultaneously
1=A
1/ El; And with light absorption value A
1With C
1Export described detection data outputting module to, finish and measure and show.
Figure 2 shows that the structural representation of the sampling and measuring device that two jackscrews are set, the distinctive points of its structure and embodiment 1 described structure is that this sampling and measuring device also is provided with second jackscrew 2, the position specifically is set is: on described on the measuring table 4, be provided with second jackscrew 2 perpendicular to also vertical perforation of described upward measuring table 4, towards a described end of measuring table 5 down, the bottom of described first jackscrew 1 is lower than the bottom of described second jackscrew 2 with respect to the described measuring table 4 of going up.
Fig. 5-1, Fig. 5-2 are the working state figure of the sampling and measuring device of present embodiment at two optical path lengths.Fig. 5-1 is the duty of this device when the maximum light path, at first with ferromagnetic cylinder 9 dead electricity of electromagnetism, elevating bracket 7 is after rising to the position concordant with the upper surface of following measuring table 5 under the effect of spring 8, change the distance between the measuring table 5 and last measuring table 4 down by regulating first jackscrew 1, and then regulate maximum optical path length; Fig. 5-2 is the duty of this device when the minimum light path, at first the ferromagnetic cylinder 9 of electromagnetism is got, elevating bracket 7 descends under the magneticaction of the ferromagnetic cylinder 9 of electromagnetism, changes the distance between the measuring tables 5 and last measuring table 4 down by regulating second jackscrew 2, and then regulates minimum optical path length.
In the present embodiment, maximum optical path length is selected 1mm for use, and minimum optical path length is selected 0.2mm for use.
Present embodiment greatly reduces the lifting accuracy requirement of electromagnet by regulating two different optical path lengths of length adjustment that two jackscrews stretch out the measuring table lower end, has reduced manufacturing cost.
When the application present embodiment carries out light absorption value and measurement of concetration, comprise equally and proofread and correct and measure two processes.Its flow process as shown in Figure 7 and Figure 8.
The correcting process of present embodiment is as shown in Figure 7:
At first, measuring table 4 aligns itself and following measuring table 5 up and down in the upset, and blank sample is measured, and obtains incident intensity I this moment
0
Then, measuring table 4 makes 5 one-tenth 90 of itself and following measuring tables in the upset
0, the 1-2ul standard solution is positioned over down the upper surface of accepting fiber bench 6 on the measuring table 5 after, measuring table 4 aligns itself and following measuring table 5 up and down in the upset, and tested standard solution is bridged on projection fiber bench 3 and the reception fiber bench 6.
If the correction coefficient of wishing to get maximum light path 1mm, at first with electromagnet 8 dead electricity, elevating bracket 7 makes projection fiber bench 3 and receives between the fiber bench 6 apart near ultimate range 1mm by the position of regulating first jackscrew after rising to the position concordant with the upper surface of following measuring table 5 under the effect of spring; Obtain the projection light intensity I of tested standard solution this moment by described photoelectric conversion module
1, described data reception module receives incident intensity I
0With projection light intensity I
1Signal after transfer to described detection computations module and calculate, the detection computations module by light absorption value A=-lg (I/I
0) while additional proportion coefficient X
0=10 correction light absorption value A that can obtain standard solution this moment
1, described detection computations module will be proofreaied and correct light absorption value A
1Be sent to described data calibration module, described data calibration module and standard light absorption value A
bObtain correction coefficient X relatively
1=A
b/ A
1, and with correction coefficient X
1Deposit in the data reception module, proofread and correct and finish;
If the correction coefficient of wishing to get minimum optical path length 0.2mm,, after elevating bracket 7 is inhaled down, make projection fiber bench 3 and receive between the fiber bench 6 apart near minor increment 0.2mm by the position of regulating second jackscrew 2 at first with electromagnet 8 energisings; Obtain the projection light intensity I of tested standard solution this moment by described photoelectric conversion module
1, described data reception module receives incident intensity I
0With projection light intensity I
1Signal after transfer to described detection computations module and calculate, the detection computations module by light absorption value A=-lg (I/I
0) while additional proportion coefficient X
0=50 correction light absorption value A that can obtain standard solution this moment
0.2, described detection computations module will be proofreaied and correct light absorption value A
1Be sent to described data calibration module, described data calibration module and standard light absorption value A
bObtain correction coefficient X relatively
0.2=A
b/ A
0.2, and with correction coefficient X
0.2Deposit in the data reception module, proofread and correct and finish.
The measuring process of present embodiment is consistent with the measuring process of embodiment 1.
Though the present invention elaborates it by embodiment; but; the any form that does not exceed the claim protection domain that those skilled in the art should be understood that on this basis to be made and the variation of details all belong to invention which is intended to be protected.
Claims (12)
1. sampling and measuring device that is used for micro-spectrophotometer, it comprises
Last measuring table (4) and following measuring table (5), described upward measuring table (4) and described distance between the measuring table (5) down are adjustable;
Measuring table on described (4) is provided with projection fiber bench (3) or receives fiber bench (6), described measuring table (5) down is provided with and receives fiber bench (6) or projection fiber bench (3), and described projection fiber bench (3) and described reception fiber bench (6) are connected with optical fiber respectively; Described projection fiber bench (3) and described reception fiber bench (6) align up and down, are used to hold sample to be checked;
On described on the measuring table (4), be provided with and be used to regulate described measuring table (4) and described first jackscrew (1) of distance between the measuring table (5) down gone up, upward be provided with the elevating bracket (7) that can move up and down and be set in outside the described elevating bracket (7) and the fixing ferromagnetic cylinder of electromagnetism (9) of described measuring table (5) down at described measuring table (5) down with the corresponding position of described first jackscrew (1); Described last measuring table (4) and described following measuring table (5) are up and down to timing, and the top of the bottom of described first jackscrew (1) and described elevating bracket (7) contacts;
It is characterized in that:
Described sampling and measuring device also comprises the detecting unit (20) that is connected with described reception fiber bench (6), and described detecting unit (20) comprises photoelectric conversion module, data reception module, detection computations module, data calibration module, optical path length selection module and detects data outputting module;
Described photoelectric conversion module is connected by grating with described reception fiber bench (6), and described photoelectric conversion module is connected with described data reception module, and the electric signal after opto-electronic conversion is inputed to described data reception module;
Described data reception module also respectively with described detection computations module, data calibration module and optical path length select module to be connected, described detection computations module receives the detection data of described data reception module output and it is calculated, described data calibration module is connected with described detection computations module, described data calibration module receives the result of calculation of described detection computations module output and to this result definite correction coefficient of comparing, and this correction coefficient inputed to described data reception module, the detection computations module is carried out light absorption value according to the correction coefficient of determining and is calculated; Described optical path length selects module to be connected with described detection computations module, described optical path length selects module to receive the result of calculation of described detection computations module output and to this result definite optical path length of comparing, and the optical path length of selecting inputed to described data reception module, the detection computations module is calculated according to corresponding described optical path length again; The detection numerical value output that described detection data outputting module calculates described detection computations module.
2. the sampling and measuring device that is used for micro-spectrophotometer according to claim 1 is characterized in that:
On described on the measuring table (4), be provided with second jackscrew (2) perpendicular to also vertical perforation of described upward measuring table (4), towards a described end of measuring table (5) down, the bottom of described first jackscrew (1) is lower than the bottom of described second jackscrew (2) with respect to the described measuring table (4) of going up.
3. the sampling and measuring device that is used for micro-spectrophotometer according to claim 1 and 2 is characterized in that:
The described measuring table (4) of going up is connected by central shaft (11) with described measuring table (5) down, and described upward measuring table (4) can be along described central shaft (11) rotating opening.
4. the sampling and measuring device that is used for micro-spectrophotometer according to claim 3 is characterized in that:
Be provided with the biasing force parts (8) that can apply biasing force towards described elevating bracket (7) in the bottom of described elevating bracket (7), the bottom of the other end of described biasing force parts (8) and the ferromagnetic cylinder of described electromagnetism (9) inside is inconsistent.
5. the sampling and measuring device that is used for micro-spectrophotometer according to claim 4 is characterized in that:
The ferromagnetic cylinder of described electromagnetism (9) when electric, obtain minimum optical path length; During the ferromagnetic cylinder of described electromagnetism (9) dead electricity, obtain maximum optical path length.
6. the sampling and measuring device that is used for micro-spectrophotometer according to claim 5 is characterized in that:
Described minimum optical path length is 0.2mm, and maximum optical path length is 1.0mm.
7. according to claim 1 or 2 or 4 or the 5 or 6 described sampling and measuring devices that are used for micro-spectrophotometer, it is characterized in that:
Measuring table on described (4) is when closing, measuring table (4) is offered groove respectively with the corresponding position on described down two planes that measuring table (5) is relative on described, place magnet (10) in described inside grooves, realize described upward measuring table (4) and the described locking of measuring table (5) down by the magnetic force of described magnet (10).
8. according to the arbitrary described sampling and measuring device of claim 1-7, it is characterized in that:
Described photoelectric conversion module is a photodetector.
9. the described using method that is used for the sampling and measuring device of micro-spectrophotometer of claim 1 may further comprise the steps:
Measuring table in the adjusting (4) aligns itself and described measuring table (5) down up and down, and blank sample is measured, and measures incident intensity I
0
The microstandard drips of solution is added on the upper surface of reception fiber bench (6) on the described down measuring table (5) or projection fiber bench (3), regulate the described measuring table (4) of going up itself and following measuring table (5) are aligned up and down, tested standard solution is bridged between described projection fiber bench (3) and the described reception fiber bench (6);
By regulating described first jackscrew, make the approaching maximum optical path length of distance between described projection fiber bench (3) and the described reception fiber bench (6); The ferromagnetic cylinder of described electromagnetism (9) is switched on, under the magneticaction of electromagnet, described first jackscrew (1) presses down described elevating bracket (7), when reaching balance, makes distance between described projection fiber bench (3) and the described reception fiber bench (6) near minimum optical path length;
After above-mentioned distance adjustment finishes, the described reception fiber bench of described grating pair inner fiber receives synthetic light signal and carries out beam split, wavelength optical signals is divided into monochromatic light, described photoelectric conversion module receives described monochromatic light and respectively its light intensity value I is converted into electric signal, described data reception module receives above-said current signal and described electric signal is handled, and the signal after the described processing transferred to described detection computations module, sample light intensity value I and blank light intensity value I after described detection computations module is according to conversion
0Obtain light absorption value A=-lg (I/I
0), at last the light absorption value that calculates is inputed to described data calibration module, in described data calibration module, the standard light absorption value A of described data calibration module invokes standard solution
bCompare, obtain correction coefficient X=A
b/ A, and after depositing correction coefficient in data reception module, proofread and correct and finish;
Described projection fiber bench (3) and described reception fiber bench (6) are cleaned, get after the cleaning on the upper surface that micro-sample drop to be checked is added to reception fiber bench (6) on the described down measuring table (5) or projection fiber bench (3), regulate the described measuring table (4) of going up itself and following measuring table (5) are aligned up and down, sample to be checked is bridged between described projection fiber bench (3) and the described reception fiber bench (6);
The ferromagnetic cylinder of electromagnetism (9) is got, obtain minimum optical path length, optical fiber in the described reception fiber bench (6) with optical signal transmission to described grating, the monochromatic light signal that synthetic light signal is divided into different wave length, described photoelectric conversion module receives described monochromatic light and respectively its light intensity value I is converted into electric signal, described data reception module receives above-said current signal and described electric signal is handled, and the signal after the described processing transferred to described detection computations module, the correction coefficient X of the minimum optical path length in the described data reception module of described detection computations module invokes, and the signal after the described processing calculated light absorption value A=-lg (I/I
0) * X, and concentration value C=A
0/ El is sent to the light absorption value A that records after described optical path length selects module, with the absorbance threshold value A
YCompare;
Record light absorption value A greater than described absorbance threshold value A
YThe time, light absorption value is measured in described detection data outputting module output and concentration value is shown to the user; Record light absorption value less than described absorbance threshold value A
YThe time, the ferromagnetic cylinder of electromagnetism (9) dead electricity, obtain maximum optical path length after, through behind the photoelectric conversion module, data reception module, detection computations module, data outputting module will be measured light absorption value and concentration value is shown to the user through detecting again.
10. the using method that is used for the sampling and measuring device of micro-spectrophotometer according to claim 9 is characterized in that:
In described step (2), after described upward measuring table (4) aligns up and down with following measuring table (5), utilize magnet (10) that described upward measuring table (4) and described measuring table (5) are down locked.
11. the using method that is used for the sampling and measuring device of micro-spectrophotometer according to claim 9 is characterized in that:
The described maximum optical path length of step (3) is 1mm, and minimum optical path length is 0.2mm.
12. the using method that is used for the sampling and measuring device of micro-spectrophotometer according to claim 9 is characterized in that:
In described step (3), get under the electric situation at the ferromagnetic cylinder of described electromagnetism (9), the described measuring table (3) of going up moves down, and by regulating described second jackscrew (2), makes the approaching minimum optical path length of distance between described projection fiber bench (3) and the described reception fiber bench (6).
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105547473A (en) * | 2016-01-19 | 2016-05-04 | 济南大学 | Spectrophotometer |
| CN105842178A (en) * | 2016-04-20 | 2016-08-10 | 华侨大学 | Non-intrusive mode online detection device for pH value of cell culture fluid and detection method |
| CN106644564A (en) * | 2016-10-12 | 2017-05-10 | 中国地质大学（武汉） | Deep-sea sediment gravity coring test stand and operation method thereof |
| CN110832303A (en) * | 2017-07-10 | 2020-02-21 | 株式会社岛津制作所 | Flame atomic absorption spectrophotometer |
| WO2023024868A1 (en) * | 2021-08-24 | 2023-03-02 | 左渡海 | Optical measurement device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001014855A1 (en) * | 1999-08-20 | 2001-03-01 | Charles William Robertson | Liquid photometer using surface tension to contain sample |
| CN1912585A (en) * | 2005-08-12 | 2007-02-14 | 深圳迈瑞生物医疗电子股份有限公司 | Method and device for automatic correcting and measuring gas concentration using infrared light absorption characteristic |
| CN101180497A (en) * | 2005-02-11 | 2008-05-14 | 纳米微滴技术公司 | Method for measuring fluorescence by surface tension |
| WO2010040104A1 (en) * | 2008-10-03 | 2010-04-08 | Nanodrop Technologies Llc | Optical path length sensor and method for optimal absorbance measurements |
| CN201955299U (en) * | 2011-03-18 | 2011-08-31 | 赵景琪 | Sampling measurement device for micro-spectrophotometer |
-
2011
- 2011-03-18 CN CN 201110066094 patent/CN102207456B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001014855A1 (en) * | 1999-08-20 | 2001-03-01 | Charles William Robertson | Liquid photometer using surface tension to contain sample |
| CN101180497A (en) * | 2005-02-11 | 2008-05-14 | 纳米微滴技术公司 | Method for measuring fluorescence by surface tension |
| CN1912585A (en) * | 2005-08-12 | 2007-02-14 | 深圳迈瑞生物医疗电子股份有限公司 | Method and device for automatic correcting and measuring gas concentration using infrared light absorption characteristic |
| WO2010040104A1 (en) * | 2008-10-03 | 2010-04-08 | Nanodrop Technologies Llc | Optical path length sensor and method for optimal absorbance measurements |
| CN201955299U (en) * | 2011-03-18 | 2011-08-31 | 赵景琪 | Sampling measurement device for micro-spectrophotometer |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105547473A (en) * | 2016-01-19 | 2016-05-04 | 济南大学 | Spectrophotometer |
| CN105842178A (en) * | 2016-04-20 | 2016-08-10 | 华侨大学 | Non-intrusive mode online detection device for pH value of cell culture fluid and detection method |
| CN106644564A (en) * | 2016-10-12 | 2017-05-10 | 中国地质大学（武汉） | Deep-sea sediment gravity coring test stand and operation method thereof |
| CN106644564B (en) * | 2016-10-12 | 2019-02-05 | 中国地质大学（武汉） | Halmeic deposit gravity column sampling test platform and operating method |
| CN110832303A (en) * | 2017-07-10 | 2020-02-21 | 株式会社岛津制作所 | Flame atomic absorption spectrophotometer |
| WO2023024868A1 (en) * | 2021-08-24 | 2023-03-02 | 左渡海 | Optical measurement device |
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