CN102206232B - Composition for plant monomer extraction and purification - Google Patents
Composition for plant monomer extraction and purification Download PDFInfo
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- CN102206232B CN102206232B CN 201010137179 CN201010137179A CN102206232B CN 102206232 B CN102206232 B CN 102206232B CN 201010137179 CN201010137179 CN 201010137179 CN 201010137179 A CN201010137179 A CN 201010137179A CN 102206232 B CN102206232 B CN 102206232B
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- phytic acid
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Abstract
The invention discloses a composition for plant monomer extraction and purification. The composition comprises, by mass, 5% to 37.5% of cellulose, 10% to 15% of galactose, 0.5% to 2% of cellulase, 45% to 63% of saponin and 7% to 15% of vitamin E. According to the composition for plant monomer extraction and purification provided in the present invention, a natural plant extract is used to purify the plant monomers, so the purity of products is improved.
Description
Technical field
The present invention relates to the composition for the plant monomer extraction and cleaning, be used in particular for the phytic acid purifying.
Background technology
Phytic acid is to exist with the phytic acid salt form in plant materials, is a kind of anti-nutrition component, and its existence causes the nutritive ingredient of plant to be difficult to be absorbed by animal, and phytic acid originally has very high value in medicine, food, daily use chemicals, environmental protection and anti-corrosion of metal.Effectively extract phytic acid, to the deep development of agricultural byproducts, improving the value of agricultural byproducts and taking full advantage of resource has great meaning.
Phytic acid is not purified, and its industrial value is little; Phytic acid is because its natural characteristics causes the technical difficulty of purification big.The liquid phytic acid is the mixture of water and phytic acid; Phytic acid and multiple organism are sequestered in together, polysaccharide, albumen, metal ion, plant colloid etc.Conventional extracting method such as the precipitator method, ion exchange method, comprise that membrane sepn etc. all is difficult to effectively separate.In the phytic acid leaching process, adopt chemical reagent such as potassium permanganate, hydrogen peroxide, yellow prussiate of potash to handle at present, but also cure the symptoms, not the disease, can only shield pigment or the impurity such as oxidation pigment of the inside, effective separating impurity, that returns product simultaneously uses of seat belts hidden danger.
Summary of the invention
The purpose of this invention is to provide a kind of high composition that is used for the plant monomer extraction and cleaning of plant monomer purity that extracts.
Composition for the plant monomer extraction and cleaning provided by the present invention is made up of Mierocrystalline cellulose, semi-lactosi, cellulase, saponin(e and vitamin-E.
Composition for the plant monomer extraction and cleaning of the present invention, wherein: calculate according to the quality percentage composition, described cellulose comprises 5%～37.5%, described semi-lactosi account for 10%～15%; Described cellulase accounts for 0.5%～2%; Described saponin accounts for 45%～63%; Described vitamin-E accounts for 7%～15%.
Composition for the plant monomer extraction and cleaning of the present invention, wherein: described cellulosic molecular weight is 50000～100000.
Composition for the plant monomer extraction and cleaning of the present invention, wherein: described saponin(e is triterpenoid saponin.
Composition for the plant monomer extraction and cleaning of the present invention adopts saponin, cellulase every impurity that dissociates, and with Mierocrystalline cellulose polymerization protein isolate and polysaccharide and other impurity, adopts the natural plant extracts phytic acid of purifying, and has improved product purity.
Embodiment
Embodiment 1, production high purity liquid phytic acid
The composition (AR-9) that is used for the plant monomer extraction and cleaning is white powder, it is the mixture of Mierocrystalline cellulose, D-semi-lactosi, cellulase, triterpenoid saponin and vitamin-E, and wherein cellulosic quality percentage composition is 5%, the quality percentage composition of D-semi-lactosi is 15%; The quality percentage composition of cellulase is 2%; The quality percentage composition of triterpenoid saponin is 63%; The quality percentage composition of vitamin-E is 15%.
With AR-9 production high purity liquid phytic acid:
The hydrochloric acid soln that adopts defatted rice bran to add 1% (volumn concentration) of its 8 times of volumes soaked 4 hours, filter, filter residue with 3 times of filter residue volumes hydrochloric acid soln soak filtration; Merge secondary filtrate; Adjust pH 3.5 with NaOH then, add activated carbon decolorizing, filter then, get filtrate; Gained filtrate enters anion-exchange column and exchanges, and 4% (quality percentage composition) NaOH wash-out is used in washing then; The liquid of wash-out is the sodium phytate mother liquor.The sodium phytate mother liquor is crossed the cationic exchange coloum exchange, and flowing liquid is concentrated into 15%, adds AR-9, the add-on of AR-9 stirred 40 minutes with 60r/min for concentrating 0.02% of the liquid weight obtain, left standstill 4 hours, added 0.5% gac, continue to stir 40min, filter; Filter to get filtrate.Filtrate is advanced macroporous anion exchange resin chromatography column chromatography, and chromatographic solution advances upgrading tower 60-65 ℃, and vacuum tightness 750mm Hg concentrates, and is concentrated into 75% colourless liquid.
Concentrated solution is colourless, transparent, limpid liquid, and getting concentrated solution, to carry out the liquid that conventional iron(ic) chloride identifies that explanation obtains be phytic acid.Metal ion adopts aas determination.Protein content adopts triumphant formula titration measuring.
Detected result is as shown in table 1.
The detected result of the every index of table 1. phytic acid
| Outward appearance | Colourless transparent liquid |
| Phytic acid purity % | 98 |
| Phytic acid content % | 75 |
| Calcium (Ca) % | 0.001 |
| Iron (Fe) % | 0.01 |
| Chlorine root (Cl) % | 0.0002 |
| Sulfate radical (SO 4)％ | ---- |
| Polysaccharide % | 0.01 |
| Inorganic phosphorus (P) % | ---- |
| Heavy metal (Pb) % | 0.002 |
| Arsenic (As) % | 0.0002 |
| Foreign protein % | 0.01 |
Embodiment 2, production high purity liquid phytic acid
The composition (AR-9) that is used for the plant monomer extraction and cleaning is white powder, it is the mixture of Mierocrystalline cellulose, L-semi-lactosi, cellulase, triterpenoid saponin and vitamin-E, and wherein cellulosic quality percentage composition is 37.5%, the quality percentage composition of L-semi-lactosi is 10%; The quality percentage composition of cellulase is 0.5%; The quality percentage composition of triterpenoid saponin is 45%; The quality percentage composition of vitamin-E is 7%.
The sulphuric acid soln that adopts the corn dregs of rice to add 3% (volumn concentration) of its 12 times of volumes soaked 6 hours, filtered, and filter residue soaks with 3% sulphuric acid soln of its 5 times of volumes, filters merging secondary filtrate; Adding activated carbon decolorizing filters; Filtrate adds NaOH and adjusts pH 4.6, separates out albumen, removes by filter albumen, gets filtrate; Gained filtrate enters anion-exchange column and exchanges, washs, and uses the 8%KOH eluant solution then; The liquid of wash-out is the POTASSIUM PHYTATE mother liquor.The POTASSIUM PHYTATE mother liquor is crossed the cationic exchange coloum exchange, and effluent liquid advances upgrading tower and is concentrated into 8%, adds AR-9, the add-on of AR-9 stirred 80 minutes with 40r/min for concentrating 0.1% of the liquid weight obtain, left standstill 8 hours, added 0.5% gac, continue to stir 60min, filter; Filter to get filtrate; Filtrate is advanced macroporous anion exchange resin chromatography column chromatography, and chromatographic solution is concentrated into 70% colourless liquid.
Concentrated solution is colourless, transparent, limpid liquid, and getting concentrated solution, to carry out the liquid that conventional iron(ic) chloride identifies that explanation obtains be phytic acid.Metal ion adopts aas determination.Protein content adopts triumphant formula titration measuring.
Detected result is as shown in table 2.
The detected result of the every index of table 2. phytic acid
| Outward appearance | Colourless transparent liquid |
| Phytic acid purity % | 98.5 |
| Phytic acid content % | 70 |
| Calcium (Ca) % | 0.001 |
| Iron (Fe) % | 0.01 |
| Chlorine root (Cl) % | 0.001 |
| Sulfate radical (SO 4)％ | 0.001 |
| Polysaccharide % | 0.009 |
| Inorganic phosphorus (P) % | 0.008 |
| Heavy metal (Pb) % | 0.0015 |
| Arsenic (As) % | 0.0002 |
| Foreign protein % | 0.01 |
Embodiment 3, production high purity liquid phytic acid
The composition (AR-9) that is used for the plant monomer extraction and cleaning is white powder, it is the mixture of Mierocrystalline cellulose, L-semi-lactosi, cellulase, triterpenoid saponin and vitamin-E, and wherein cellulosic quality percentage composition is 20%, the quality percentage composition of L-semi-lactosi is 12%; The quality percentage composition of cellulase is 1%; The quality percentage composition of triterpenoid saponin is 54%; The quality percentage composition of vitamin-E is 13%.
The citric acid solution that adopts defatted rice bran to add 2% (volumn concentration) of 10 times of volumes soaked 5 hours, filtered, and filter residue soaks with 2% citric acid solution of 4 times of volumes, filters merging secondary filtrate; The adding activated carbon decolorizing filters, filtrate adds NaOH and adjusts pH value to 3.3, separates out albumen, removes by filter albumen, gets filtrate; Gained filtrate is advanced anion-exchange column exchange, washing, uses 6%NaHCO then
3Eluant solution; The liquid of wash-out is the sodium phytate mother liquor.The sodium phytate mother liquor is crossed cationic exchange coloum exchange current fluid and is advanced upgrading tower and concentrate, be concentrated into 10% liquid that obtains and add AR-9, the add-on of AR-9 stirred 60 minutes with 50r/min for concentrating 0.06% of the liquid weight obtain, left standstill 6 hours, the gac of adding 0.5%, continue to stir 60min, filter; Filter to get filtrate.Filtrate is advanced macroporous anion exchange resin chromatography column chromatography, and chromatographic solution continues to be concentrated into 50%.
Concentrated solution is colourless, transparent, limpid liquid, and getting concentrated solution, to carry out the liquid that conventional iron(ic) chloride identifies that explanation obtains be phytic acid.Metal ion adopts aas determination.Protein content adopts triumphant formula titration measuring.
Detected result is as shown in table 3.
The detected result of the every index of table 3. phytic acid
| Outward appearance | Colourless transparent liquid |
| Phytic acid purity % | 98.6 |
| Phytic acid content % | 50 |
| Calcium (Ca) % | 0.001 |
| Iron (Fe) % | 0.01 |
| Chlorine root (Cl) % | 0.001 |
| Sulfate radical (SO 4)％ | 0.001 |
| Polysaccharide % | 0.008 |
| Inorganic phosphorus (P) % | 0.01 |
| Heavy metal (Pb) % | 0.002 |
| Arsenic (As) % | 0.0002 |
| Foreign protein % | 0.008 |
Above embodiment is described preferred implementation of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that the common engineering technical personnel in this area make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (1)
1. the composition that is used for the plant monomer extraction and cleaning, formed by Mierocrystalline cellulose, semi-lactosi, cellulase, saponin(e and vitamin-E, calculate according to the quality percentage composition, described cellulose comprises 5%～37.5%, described semi-lactosi accounts for 10%～15%, described cellulase accounts for 0.5%～2%, and described saponin accounts for 45%～63%, and described vitamin-E accounts for 7%～15%;
Wherein, described cellulosic molecular weight is between 50,000～100,000; Described saponin(e is triterpenoid saponin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010137179 CN102206232B (en) | 2010-03-31 | 2010-03-31 | Composition for plant monomer extraction and purification |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010137179 CN102206232B (en) | 2010-03-31 | 2010-03-31 | Composition for plant monomer extraction and purification |
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| CN102206232A CN102206232A (en) | 2011-10-05 |
| CN102206232B true CN102206232B (en) | 2013-08-21 |
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| CN 201010137179 Expired - Fee Related CN102206232B (en) | 2010-03-31 | 2010-03-31 | Composition for plant monomer extraction and purification |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563019A (en) * | 2004-04-09 | 2005-01-12 | 夏红 | Method of technique of ion exchange for extracting phytic acid |
| CN101602776A (en) * | 2009-02-23 | 2009-12-16 | 杜江 | The production method of high-purity solid phytic acid |
-
2010
- 2010-03-31 CN CN 201010137179 patent/CN102206232B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563019A (en) * | 2004-04-09 | 2005-01-12 | 夏红 | Method of technique of ion exchange for extracting phytic acid |
| CN101602776A (en) * | 2009-02-23 | 2009-12-16 | 杜江 | The production method of high-purity solid phytic acid |
Non-Patent Citations (4)
| Title |
|---|
| 梁少华等.植酸提取方法的研究.《郑州粮食学院学报》.1997,第18卷(第3期),第86-91页. |
| 植酸提取方法的研究;梁少华等;《郑州粮食学院学报》;19970930;第18卷(第3期);第86-91页 * |
| 王文侠等.离子吸脱法从脱脂米糠中提取植酸的研究.《齐齐哈尔大学学报》.2005,第21卷(第1期),第19-22页. |
| 离子吸脱法从脱脂米糠中提取植酸的研究;王文侠等;《齐齐哈尔大学学报》;20050331;第21卷(第1期);第19-22页 * |
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