CN102187856A - Fresh flower preservative - Google Patents

Fresh flower preservative Download PDF

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Publication number
CN102187856A
CN102187856A CN201110082069XA CN201110082069A CN102187856A CN 102187856 A CN102187856 A CN 102187856A CN 201110082069X A CN201110082069X A CN 201110082069XA CN 201110082069 A CN201110082069 A CN 201110082069A CN 102187856 A CN102187856 A CN 102187856A
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fresh
preservative
percent
garlic
olive leaf
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CN102187856B (en
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韩金光
伍曾利
吴家强
王海生
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Hainan standard biological Polytron Technologies Inc
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韩金光
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Abstract

The invention discloses a fresh flower preservative, which comprises the following components in percentage by weight: 0.5 to 4.0 percent of sucrose, 0.01 to 0.05 percent of 8-hydroxyquinoline sulfate, 0.001 to 0.004 percent of 1-methyl cyclopropene, 0.0005 to 0.002 percent of gibberellin, 0.01 to 0.06 percent of calcium nitrate, 0.05 to 0.3 percent of garlic extract, 0.05 to 0.2 percent of olive leaf extract, 0.03 to 0.15 percent of citric acid, 0.001 to 0.004 percent of 6-benzylaminopurine, and the balance of water. The invention also discloses a preparation method of the fresh flower preservative. The novel biological preservative (carnosine) is blended with the traditional additive, so that the defects of the conventional fresh flower preservative are overcome; the degradability advantage of the biological preservative is used; the fresh flower preservative is a product which is convenient to use and has good fresh keeping effect and no toxic hazard effect; and the fresh flower preservative meets the requirements of customers on spiritual civilization, and a technical scheme and a product are provided for solving the problem of difficulty in keeping flowers fresh.

Description

Fresh-preservative
Technical field
The present invention relates to flowers cultural technique field, especially a kind of fresh-preservative.
Background technology
In recent years, raising along with living standards of the people, great variation has taken place in the Chinese flower production and consumption, flowers become ordinary citizen's consumption fashion just gradually, flower industry is because huge economic benefit and potential domestic and international market simultaneously, become one of industry of current tool development prospect, be described as " the gold industry " of 21 century.
As a subsidiary industry of flower industry, along with the increase of people's fresh flower consumption figure, the fresh-keeping industry of fresh flower is prosperous just gradually.The fresh-keeping formulation products of China is single at present, adopts pure chemistry reagent to be used as fresh-preservative, and environmental pollution is serious, and problems such as DeGrain are very outstanding.Therefore, carrying out the post-harvest fresh-keeping of fresh flower, for the sustainable development of whole cut-flower industry, is vital.
Summary of the invention
The present invention is directed to deficiency, propose a kind of fresh-preservative, adopt the composition of biological components, reduced the pollution of antistaling agent, improved fresh-keeping effect simultaneously environment as antistaling agent.
In order to realize the foregoing invention purpose, the invention provides following technical scheme: a kind of fresh-preservative is made of the following component mixing of weight percentage:
Figure BDA0000053503110000011
Figure BDA0000053503110000021
Preferably, described garlic P.E is to obtain via following preparation process:
Get garlic, clean, the water that adds 4~8 times of amounts of garlic is pulled an oar; Adjusting slurry pH value to 3.5~5.5, temperature control be at 40~60 ℃, adds 20~60 million international units by the per kilogram garlic and carry out enzymolysis than the enzyme of vigor, and enzymolysis time is 180~360 minutes, and enzymolysis carries out enzyme-deactivating after finishing; Isolate clear liquid, last chromatography column separating purification is crossed milipore filter and is collected filter liquor, concentrates, and atomized drying promptly gets garlic P.E.
Preferably, described enzyme by cellulase, amylase, hemicellulase and pectase by weight 1~3: 1~2: 1~3: 1~2 is composited.
Preferably, described chromatographic column separation and purification process is: on the clear liquid behind the chromatographic column, water cleans removal of impurities, is 5.5~7.0 phosphate buffer wash-out again with the pH value of 20mmo1/L, collection phosphate eluent.
Preferably, described milipore filter is the daltonian milipore filters in molecular weight 5~100,000.
Preferably, described chromatographic column is the hydroxyapatite chromatography post.
Preferably, described Olive leaf P.E is to obtain via following preparation process:
Get dry olive leaf, pulverize, the water that adds 6~12 times of weight of olive leaf carries out Microwave Extraction, and extraction time is 60min~240min; Supernatant is got in centrifugal filtration, concentrates, and atomized drying gets Olive leaf P.E.
Compared with prior art, the present invention adopts the new bio antistaling agent, as Olive leaf P.E, the carnosine that wherein contains adds conventional additive and allocates, and has overcome the shortcoming of existing fresh-preservative, utilize the degradability advantage of bio-preservative, be the fresh-preservative product of a kind of good refreshing effect, nonhazardous effect, satisfy the demand of consumer, for the difficult problem of flower freshness provides a kind of technical scheme and product for spiritual civilization.Be applicable to storage, transportation, sale and flower arrangement after fresh flowers shearings such as lily, rose, China rose, carnation, chrysanthemum are exsomatized.
The making of fresh-preservative of the present invention, only need take by weighing each component in proportion and mix and get final product.Whole process is simple, is very easy to.
Embodiment
Describe the present invention below in conjunction with specific embodiment, the description of this part only is exemplary and explanatory, should any restriction not arranged to protection scope of the present invention.
Embodiment 1
A kind of novel fresh-preservative takes by weighing following components in weight percentage respectively, and each component is mixed, and promptly obtains fresh-preservative:
Sucrose 0.5%, oxine sulphate 0.01%, 1-methyl cyclopropene 0.001%, gibberellin 0.0005%, nitrate of lime 0.01%, garlic P.E 0.05%, Olive leaf P.E 0.05%, citric acid 0.03%, 6-benzyl aminopurine 0.001%, all the other are water.
Wherein garlic P.E is to obtain via following preparation process: get garlic, clean, the water that adds 8 times of amounts of garlic is pulled an oar; Adjusting slurry pH value to 3.5, temperature control be at 60 ℃, adds 20 million international units by the per kilogram garlic and carry out enzymolysis than the enzyme of vigor, and enzymolysis time is 360 minutes, and enzymolysis carries out enzyme-deactivating after finishing; Isolate clear liquid, last chromatography column separating purification is crossed milipore filter and is collected filter liquor, concentrates, and atomized drying promptly gets garlic P.E.
Olive leaf P.E is to obtain via following preparation process: get dry olive leaf, pulverize, the water that adds 6 times of weight of olive leaf carries out Microwave Extraction, and extraction time is 240min; Supernatant is got in centrifugal filtration, concentrates, and atomized drying gets Olive leaf P.E.
The fresh-preservative that makes with embodiment 1 is fresh-keeping group, organizes in contrast with water, the fresh-keeping test of arranging flowers after selection Fragrant Lily flower, rose, China rose and carnation are sheared and exsomatize, comparative test result such as table 1.
The fresh-keeping result of the test of table 1:
Annotate: reduced value is a fresh-keeping group/contrast groups, down together.
Embodiment 2
A kind of novel fresh-preservative takes by weighing following components in weight percentage respectively, and each component is mixed, and promptly obtains fresh-preservative:
Sucrose 4.0%, oxine sulphate 0.05%, 1-methyl cyclopropene 0.004%, gibberellin 0.002%, nitrate of lime 0.06%, garlic P.E 0.3%, Olive leaf P.E 0.2%, citric acid 0.15%, 6-benzyl aminopurine 0.004%, all the other are water.
Wherein, garlic P.E is to obtain via following preparation process: get garlic, clean, the water that adds 4 times of amounts of garlic is pulled an oar; Adjusting slurry pH value to 5.5, temperature control be at 40 ℃, adds 60 million international units by the per kilogram garlic and carry out enzymolysis than the enzyme of vigor, and enzymolysis time is 180 minutes, and enzymolysis carries out enzyme-deactivating after finishing; Isolate clear liquid, last chromatography column separating purification is crossed milipore filter and is collected filter liquor, concentrates, and atomized drying promptly gets garlic P.E.
Olive leaf P.E is to obtain via following preparation process: get dry olive leaf, pulverize, the water that adds 12 times of weight of olive leaf carries out Microwave Extraction, and extraction time is 60min; Supernatant is got in centrifugal filtration, concentrates, and atomized drying gets Olive leaf P.E.
The fresh-preservative that makes with embodiment 2 is fresh-keeping group, organizes in contrast with water, the fresh-keeping test of arranging flowers after selection Fragrant Lily flower, rose, China rose and carnation are sheared and exsomatize, comparative test result such as table 2.
The fresh-keeping result of the test of table 2:
Figure BDA0000053503110000051
Embodiment 3
A kind of novel fresh-preservative takes by weighing following components in weight percentage respectively, and each component is mixed, and promptly obtains fresh-preservative:
Sucrose 2.25%, oxine sulphate 0.03%, 1-methyl cyclopropene 0.0025%, gibberellin 0.00125%, nitrate of lime 0.035%, garlic P.E 0.175%, Olive leaf P.E 0.125%, citric acid 0.09%, 6-benzyl aminopurine 0.0025%, all the other are water.
Wherein, garlic P.E is to obtain via following preparation process: get garlic, clean, the water that adds 6 times of amounts of garlic is pulled an oar; Adjusting slurry pH value to 4.5, temperature control be at 50 ℃, adds 40 million international units by the per kilogram garlic and carry out enzymolysis than the enzyme of vigor, and enzymolysis time is 240 minutes, and enzymolysis carries out enzyme-deactivating after finishing; Isolate clear liquid, last chromatography column separating purification is crossed milipore filter and is collected filter liquor, concentrates, and atomized drying promptly gets garlic P.E.
Olive leaf P.E is to obtain via following preparation process: get dry olive leaf, pulverize, the water that adds 9 times of weight of olive leaf carries out Microwave Extraction, and extraction time is 150min; Supernatant is got in centrifugal filtration, concentrates, and atomized drying gets Olive leaf P.E.
The fresh-preservative that makes with embodiment 3 is fresh-keeping group, organizes in contrast with water, the fresh-keeping test of arranging flowers after selection Fragrant Lily flower, rose, China rose and carnation are sheared and exsomatize, comparative test result such as table 3.
The fresh-keeping result of the test of table 3:
Figure BDA0000053503110000061
From above-mentioned three insurance result of the test tables, used the prepared fresh-preservative of the embodiment of the invention, the bottle that has improved various fresh flowers is inserted the flower diameter of life-span and fresh flower.
It below only is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (7)

1. fresh-preservative is made of the following component mixing of weight percentage:
Figure FDA0000053503100000011
2. fresh-preservative as claimed in claim 1 is characterized in that: described garlic P.E is to obtain via following preparation process:
Get garlic, clean, the water that adds 4~8 times of amounts of garlic is pulled an oar; Adjusting slurry pH value to 3.5~5.5, temperature control be at 40~60 ℃, adds 20~60 million international units by the per kilogram garlic and carry out enzymolysis than the enzyme of vigor, and enzymolysis time is 180~360 minutes, and enzymolysis carries out enzyme-deactivating after finishing; Isolate clear liquid, last chromatography column separating purification is crossed milipore filter and is collected filter liquor, concentrates, and atomized drying promptly gets garlic P.E.
3. fresh-preservative as claimed in claim 2 is characterized in that: described enzyme by cellulase, amylase, hemicellulase and pectase by weight 1~3: 1~2: 1~3: 1~2 is composited.
4. fresh-preservative as claimed in claim 2, it is characterized in that: described chromatographic column separation and purification process is: on the clear liquid behind the chromatographic column, water cleans removal of impurities, is 5.5~7.0 phosphate buffer wash-out again with the pH value of 20mmol/L, collection phosphate eluent.
5. fresh-preservative as claimed in claim 2 is characterized in that: described milipore filter is the daltonian milipore filters in molecular weight 5~100,000.
6. fresh-preservative as claimed in claim 2 is characterized in that: described chromatographic column is the hydroxyapatite chromatography post.
7. fresh-preservative as claimed in claim 1 is characterized in that: described Olive leaf P.E is to obtain via following preparation process:
Get dry olive leaf, pulverize, the water that adds 6~12 times of weight of olive leaf carries out Microwave Extraction, and extraction time is 60min~240min; Supernatant is got in centrifugal filtration, concentrates, and atomized drying gets Olive leaf P.E.
CN 201110082069 2011-04-01 2011-04-01 Fresh flower preservative Expired - Fee Related CN102187856B (en)

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Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102550658A (en) * 2012-01-23 2012-07-11 王�琦 Chitosan film antistaling agent, its preparation method and uses
CN104012525A (en) * 2014-06-23 2014-09-03 临沂大学 Dendrobium nobile cut-flower pretreating solution formula and vase arrangement liquid formula
CN104170819A (en) * 2014-07-29 2014-12-03 卞佳林 Fresh flower preservative and preparation method of fresh flower preservative
CN104488858A (en) * 2014-12-22 2015-04-08 闽南师范大学 Multifunctional fresh cut flower gel preservative and using method thereof
CN104756983A (en) * 2015-03-23 2015-07-08 苏州谷力生物科技有限公司 Fresh flower antistaling agent and preparation method thereof
CN104970008A (en) * 2015-06-30 2015-10-14 柳州市天姿园艺有限公司 Vase solution of vanda cut-flower
CN104970006A (en) * 2015-06-30 2015-10-14 柳州市天姿园艺有限公司 Preservative of dendrobium cut-flower
CN104970009A (en) * 2015-06-30 2015-10-14 柳州市天姿园艺有限公司 Vase solution of dendrobium cut-flower
CN104982429A (en) * 2015-06-30 2015-10-21 柳州市天姿园艺有限公司 Preservative solution for cut flowers of phalaenopsis aphrodite Rchb. F.
CN105028392A (en) * 2015-06-30 2015-11-11 柳州市天姿园艺有限公司 Vase solution for cut phalaenopsis amabilis
CN105028393A (en) * 2015-06-30 2015-11-11 柳州市天姿园艺有限公司 Preservative for cut paphiopedilum flower
CN105076118A (en) * 2015-09-22 2015-11-25 宜宾云辰乔木园林有限责任公司 Organic preservative for fresh cut flowers
CN105685213A (en) * 2016-01-22 2016-06-22 新疆农业科学院园艺作物研究所 Preservative for prolonging shelf life of grapes or fresh cut flowers and preparation method and use method thereof
CN105685020A (en) * 2014-11-25 2016-06-22 广西大学 Fresh flower freshness-keeping agent
CN106818759A (en) * 2017-01-19 2017-06-13 南京农业大学 Application of the carnosine in turfgrass saline-alkaline tolerance is improved
CN106982824A (en) * 2017-06-07 2017-07-28 合肥申沃园艺有限公司 A kind of flower antistaling agent and preparation method thereof
CN107114360A (en) * 2017-06-23 2017-09-01 仲恺农业工程学院 A kind of method of gladiolus Dry pressed flowers pink colour reduction
CN107372477A (en) * 2017-09-02 2017-11-24 赖进九 A kind of agricultural composite medicine fresh-keeping for cutting flower
CN107821387A (en) * 2017-10-27 2018-03-23 陕西道大格生物科技有限责任公司 A kind of fresh-preservative
CN108935658A (en) * 2018-08-31 2018-12-07 镇江鑫源达园艺科技有限公司 A kind of agent keeping vegetable fresh and preparation method thereof
US10375954B2 (en) * 2013-01-15 2019-08-13 Agrofresh Inc. Compositions and methods for stabilizing cyclopropene in solutions

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1129058A (en) * 1995-09-30 1996-08-21 赵国晶 Non-toxic fresh-preservative and preparation method thereof
CN1153593A (en) * 1996-11-22 1997-07-09 单程仪 Fresh keeping agent for flowers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1129058A (en) * 1995-09-30 1996-08-21 赵国晶 Non-toxic fresh-preservative and preparation method thereof
CN1153593A (en) * 1996-11-22 1997-07-09 单程仪 Fresh keeping agent for flowers

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102550658A (en) * 2012-01-23 2012-07-11 王�琦 Chitosan film antistaling agent, its preparation method and uses
US10375954B2 (en) * 2013-01-15 2019-08-13 Agrofresh Inc. Compositions and methods for stabilizing cyclopropene in solutions
CN104012525A (en) * 2014-06-23 2014-09-03 临沂大学 Dendrobium nobile cut-flower pretreating solution formula and vase arrangement liquid formula
CN104170819A (en) * 2014-07-29 2014-12-03 卞佳林 Fresh flower preservative and preparation method of fresh flower preservative
CN105685020A (en) * 2014-11-25 2016-06-22 广西大学 Fresh flower freshness-keeping agent
CN104488858A (en) * 2014-12-22 2015-04-08 闽南师范大学 Multifunctional fresh cut flower gel preservative and using method thereof
CN104756983A (en) * 2015-03-23 2015-07-08 苏州谷力生物科技有限公司 Fresh flower antistaling agent and preparation method thereof
CN105028393A (en) * 2015-06-30 2015-11-11 柳州市天姿园艺有限公司 Preservative for cut paphiopedilum flower
CN104970008A (en) * 2015-06-30 2015-10-14 柳州市天姿园艺有限公司 Vase solution of vanda cut-flower
CN105028392A (en) * 2015-06-30 2015-11-11 柳州市天姿园艺有限公司 Vase solution for cut phalaenopsis amabilis
CN104970009A (en) * 2015-06-30 2015-10-14 柳州市天姿园艺有限公司 Vase solution of dendrobium cut-flower
CN104970006A (en) * 2015-06-30 2015-10-14 柳州市天姿园艺有限公司 Preservative of dendrobium cut-flower
CN104982429A (en) * 2015-06-30 2015-10-21 柳州市天姿园艺有限公司 Preservative solution for cut flowers of phalaenopsis aphrodite Rchb. F.
CN105076118A (en) * 2015-09-22 2015-11-25 宜宾云辰乔木园林有限责任公司 Organic preservative for fresh cut flowers
CN105685213A (en) * 2016-01-22 2016-06-22 新疆农业科学院园艺作物研究所 Preservative for prolonging shelf life of grapes or fresh cut flowers and preparation method and use method thereof
CN106818759A (en) * 2017-01-19 2017-06-13 南京农业大学 Application of the carnosine in turfgrass saline-alkaline tolerance is improved
CN106818759B (en) * 2017-01-19 2019-03-29 南京农业大学 Carnosine is improving the application in turfgrass saline-alkaline tolerance
CN106982824A (en) * 2017-06-07 2017-07-28 合肥申沃园艺有限公司 A kind of flower antistaling agent and preparation method thereof
CN107114360A (en) * 2017-06-23 2017-09-01 仲恺农业工程学院 A kind of method of gladiolus Dry pressed flowers pink colour reduction
CN107114360B (en) * 2017-06-23 2020-07-07 仲恺农业工程学院 Method for reducing planar dry pink gladiolus hybridus var gladiolus
CN107372477A (en) * 2017-09-02 2017-11-24 赖进九 A kind of agricultural composite medicine fresh-keeping for cutting flower
CN107821387A (en) * 2017-10-27 2018-03-23 陕西道大格生物科技有限责任公司 A kind of fresh-preservative
CN108935658A (en) * 2018-08-31 2018-12-07 镇江鑫源达园艺科技有限公司 A kind of agent keeping vegetable fresh and preparation method thereof

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