CN102178739B - Method for extracting flavone by using surfactant from herb to coordinate with enzymolysis-microwave - Google Patents
Method for extracting flavone by using surfactant from herb to coordinate with enzymolysis-microwave Download PDFInfo
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- CN102178739B CN102178739B CN 201110093463 CN201110093463A CN102178739B CN 102178739 B CN102178739 B CN 102178739B CN 201110093463 CN201110093463 CN 201110093463 CN 201110093463 A CN201110093463 A CN 201110093463A CN 102178739 B CN102178739 B CN 102178739B
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Abstract
The invention provides a method for extracting flavone by using surfactant from a herb to coordinate with enzymolysis-microwave. The method includes the following steps: (1) stirring and mixing the herb with cellulose, the surfactant and water; (2) putting the mixture obtained in step (1) in a microwave device, conducting microwave extraction for 2.0-3.0min, cooling, decompressing and filtering to acquire filtrate that is solution containing flavone. The method has no over high requirement on the granularity of medicinal materials, evenly heats an extraction object, is high-efficiency and quick, saves solvent consumption and reduces energy consumption. By using the solubilization of the surfactant and the wall breaking function of an enzymic preparation and taking water solution as a substitute for organic solvent, the method is low in cost and small in energy consumption and good in product quality.
Description
Technical field
The present invention relates to a kind of from herbaceous plant the method for extracting effective components.
Background technology
The extraction of Effective Component of Chinese Medicine is that Chinese medicine is produced the most important link of fundamental sum, also is the important topic that the Study on extraction worker pays close attention to.Because draft class medicinal herb components is complicated, the content of effective constituent is generally lower, only is about 3% such as the effective constituent in the White Mulberry Root-bark.
Traditional extraction and separation technology, such as pickling process, percolation, decocting method, soxhlet extraction method, the shortcoming such as ubiquity efficient is low, energy consumption is high, operation is many, the production cycle is long is difficult to adapt to the requirement of the modernization of Chinese medicine.Therefore, some new extraction and separation technologies arise at the historic moment.
Microwave-assisted extracts (Microwave-assisted Extraction, MAE), claims again microwave extracting (Microwave Extraction), is the combine a kind of novel sample pretreatment means of rear formation of microwave and traditional solvent-extraction process.Its principle of heating is to utilize micro-wave energy, directly acts on simultaneously the material molecule, and whole material is heated simultaneously, i.e. " body heating ".Be different from tradition and extract the characteristics of heat and mass transfer opposite direction, it is by dipole rotation and the inside and outside simultaneously heating of ionic conduction dual mode that microwave-assisted extracts, so that vegetable cell breaks, the effective constituent Fast Stripping, this Mass And Heat Transfer Process direction is identical.The advantages such as therefore, microwave technology has rapidly and efficiently, homogeneous heating, saving solvent, technique are simple, reduce investment outlay.
Summary of the invention
The purpose of this invention is to provide a kind of method of from herbaceous plant, utilizing tensio-active agent coordinated enzymatic hydrolysis-microwave extraction flavones, to satisfy the needs of relevant field development.
Method of the present invention comprises the steps:
(1) herbaceous plant and cellulase, tensio-active agent and water are mixed, stirring velocity is 0-2600r/min, processes 45～90 minutes, and makes the effective constituent stripping in the herbaceous plant for 45～54 ℃;
Described herbaceous plant is White Mulberry Root-bark or Yerbadetajo Herb;
The content of tensio-active agent in water is 1.0～2.4g/L water, and described tensio-active agent is selected from polysorbate60;
The content of cellulase in water is 0.50-1.25g/L water, the preferred green wooden enzyme of described cellulase, as to choose the production code member that extra large pattern ocean Bioisystech Co., Ltd produces be M0030, CAS number is the cellulase of 9012-54-8;
The weight of herbaceous plant in water is 0.65～1.75g/25mL;
(2) mixture that step (1) is obtained is put into microwave device, microwave extraction 2.0～3.0min, and then cooling, decompress filter, filtrate being of acquisition contained the solution of flavones.
Described microwave extraction device comprises conventional household microwave oven and is arranged on the extraction element of described household microwave oven routine, household microwave oven such as ER-692 type household microwave oven, and output rating is 500～1000W, preferred 650W, frequency 2450MHz;
The method that the present invention adopts tensio-active agent and cellulase to combine with microwave extraction, to reduce the interfacial tension of solid-liquid phase, ability is oozed out in the dissolving that increases larger molecular organics matter, can shorten infiltrating time, improve extraction rate, the mild condition that has simultaneously Enzymatic Extraction, easy and simple to handle, the advantage such as with low cost can improve the extraction yield of effective ingredient greatly.
Method of the present invention, the medicinal material granularity there is not excessive demand, to extracting the object homogeneous heating, efficient quick is saved solvent load, is reduced energy consumption, utilizes the solublization of tensio-active agent and the broken wall effect of zymin, replace organic solvent with the aqueous solution, cost is low, and energy consumption is little, and the products obtained therefrom quality is good.
Embodiment
Embodiment 1
From White Mulberry Root-bark, extract white mulberry rootbark general flavone:
(1) is 20 purpose White Mulberry Root-bark medicinal materials with the 5g granularity, places there-necked flask, add 165mL deionized water, cellulase and polysorbate60,45 ℃ of lower 45min that stir;
The content of cellulase is 0.75g/L, and the content of polysorbate60 is 2.0g/L;
(2) product that step (1) is obtained, microwave extraction 2.83min, 5min is to room temperature in cooling, shakes up decompress filter;
Microwave oven output power is 650W, frequency 2450MHz
(3) with the resulting extracting solution of step (2), use ultraviolet spectrophotometry, under the 510nm wavelength, measure flavones content, it is 510nm that described ultraviolet spectrophotometer is set wavelength, and flavones content is 0.206g/L.
Embodiment 2
From Yerbadetajo Herb, extract the Yerbadetajo Herb total flavones:
(1) is 10 purpose Yerbadetajo Herb medicinal materials with the 5g granularity, places there-necked flask, add the 105mL deionized water, cellulase, polysorbate60,54 ℃ of lower 90min that stir;
The content of cellulase is 1.00g/L, and the content of polysorbate60 is 2.4g/L;
(2) product that step (1) is obtained, microwave extraction 2.36min, 5min is to normal temperature in cooling, shakes up decompress filter;
Microwave oven output power is 650W, frequency 2450MHz.
(3) with the resulting extracting solution of step (2), use ultraviolet spectrophotometry, under the 510nm wavelength, measure flavones content, flavones content is 0.657g/L.
Claims (4)
1. from herbaceous plant, utilize the method for tensio-active agent coordinated enzymatic hydrolysis-microwave extraction flavones, it is characterized in that, comprise the steps:
(1) herbaceous plant and cellulase, tensio-active agent and water are mixed;
(2) mixture that step (1) is obtained is put into the microwave extraction device, microwave extraction 2.0～3.0min, and then cooling, decompress filter, filtrate being of acquisition contained the solution of flavones;
Described herbaceous plant is White Mulberry Root-bark or Yerbadetajo Herb.
2. method according to claim 1 is characterized in that, the stirring velocity of step (1) is 0-2600r/min, processes 45～90 minutes for 45～54 ℃.
3. method according to claim 1 is characterized in that, the content of tensio-active agent in water is 1.0～2.4g/L water, and described tensio-active agent is selected from polysorbate60; The content of cellulase in water is 0.50-1.25g/L water, and described cellulase is green wooden enzyme; The weight of herbaceous plant in water is 0.65～1.75g/25mL.
4. method according to claim 3 is characterized in that, the output rating of described microwave extraction device is 500～1000W, frequency 2450MHz.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110093463 CN102178739B (en) | 2011-04-14 | 2011-04-14 | Method for extracting flavone by using surfactant from herb to coordinate with enzymolysis-microwave |
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| CN 201110093463 CN102178739B (en) | 2011-04-14 | 2011-04-14 | Method for extracting flavone by using surfactant from herb to coordinate with enzymolysis-microwave |
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| CN102178739A CN102178739A (en) | 2011-09-14 |
| CN102178739B true CN102178739B (en) | 2013-04-10 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102489038B (en) * | 2011-11-29 | 2014-09-24 | 华东理工大学 | Method for extracting flavones in Chinese herbal medicine by surfactant cooperated supersonic wave-enzymatic hydrolysis |
| CN103243139B (en) * | 2013-05-21 | 2014-10-22 | 河南工业大学 | Method for efficiently degrading lignocelluloses raw material |
| CN104706553B (en) * | 2015-02-11 | 2018-06-05 | 广州赛莱拉干细胞科技股份有限公司 | A kind of extracting method of myrrh leaf cell extract and its application |
| CN105231249A (en) * | 2015-09-17 | 2016-01-13 | 暨南大学 | Bamboo leaf flavone extracting method by synergy of ultrasonic and surface-active agent |
| CN106377453B (en) * | 2016-08-31 | 2020-12-25 | 海南师范大学 | Morinda citrifolia extract emulsion and preparation method and application thereof |
| CN113866327A (en) * | 2016-12-12 | 2021-12-31 | 邢玉伟 | Application of LHJ solubilizer in quantitative determination of insoluble food additives |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101912445A (en) * | 2010-08-26 | 2010-12-15 | 西北农林科技大学 | Method for extracting general flavonoids from toona sinensis leaves under assistance of surfactant |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101912445A (en) * | 2010-08-26 | 2010-12-15 | 西北农林科技大学 | Method for extracting general flavonoids from toona sinensis leaves under assistance of surfactant |
Non-Patent Citations (2)
| Title |
|---|
| 丁利君等.微波-纤维素酶联合提取芒萁黄酮及抗氧化研究.《食品科技》.2011,第36卷(第1期),第172-176页. * |
| 董彩军等.黄酮类化合物的研究进展.《农产品加工.学刊》.2010,(第199期),第65-69页. * |
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