CN102174715A - Method for recycling hemp strain-containing degumming liquid for degumming hemp - Google Patents

Method for recycling hemp strain-containing degumming liquid for degumming hemp Download PDF

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Publication number
CN102174715A
CN102174715A CN 201010611429 CN201010611429A CN102174715A CN 102174715 A CN102174715 A CN 102174715A CN 201010611429 CN201010611429 CN 201010611429 CN 201010611429 A CN201010611429 A CN 201010611429A CN 102174715 A CN102174715 A CN 102174715A
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hemp
degumming
bacterium
pond
baths
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CN 201010611429
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CN102174715B (en
Inventor
蔡柏岩
凌宏志
葛菁萍
平文祥
接伟光
王贵宾
江云飞
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Heilongjiang University
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Heilongjiang University
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Abstract

The invention relates to a method for recycling hemp strain-containing degumming liquid for degumming hemp, relating to a hemp degumming process method and mainly solving the problems of the traditional hemp degumming method, such as long degumming period, low fiber yield, low hemp fiber strength and large sewage discharge during retting. The method disclosed by the invention comprises the following steps of: activating strains used for degumming hemp; carrying out primary seed culturing; carrying out second-degree seed culturing; carrying out third-degree seed culturing; degumming hemp with strains; recycling hemp strain-containing degumming liquid and adding to a new hemp degumming pond, and then adding hemp stalks, sodium chloride and urea, filling clean water into the hemp degumming pond, increasing the temperature for retting and completing the degumming process when a retting terminal point is reached. By adopting the method disclosed by the invention, 50% of waste water discharge can be reduced every day, energy consumed for degumming hemp is saved by 10%-15%; meanwhile, the degumming period of the hemp stalks is shortened to 80-120h, the hemp fiber yield is improved by 3%-5%, the hemp fiber strength is enhanced to 220-280N, and the quality of hemp fiber is improved.

Description

Hemp adds the method for bacterium degumming baths recycling carrying out hemp degumming
Technical field
The present invention relates to the method for hemp degumming.
Background technology
China adopts hemp degumming method to be at present: the wisp that at first big anesthesia stem is bundled into 20 centimeters diameters, to tie good big anesthesia stem then packs into and comes unstuck that loading density is 75~80 kilograms/cubic metre in the pond, the pond of will coming unstuck is then filled with, fill with the back and heat to needed temperature for the retted fibre pond with steam, mend temperature once to desired temperature with steam every day in the hemp degumming process.What adopt during terminal point in the judgement system of macerating is organoleptic method, and organoleptic method mainly just is meant " method of putting forth ": the straw of finishing in the position system of will macerating of the root 1/3rd of straw fractures, and xylem that can be wherein be arrival and macerate the system terminal point by extracting out in the fiber portion.
The fermentation period time that hemp warm water comes unstuck is long: have nothing in common with each other kind and the harvest time for the big anesthesia stem of hemp raw material plant at present, cause the composition of fiber in the diameter of big anesthesia stem and the bast to have nothing in common with each other, this is mainly reflected in the different of temperature and usually time of coming unstuck, and the degumming tech operative norm of each raw material plant neither be very consistent.The temperature of coming unstuck of general big anesthesia stem is 30 ℃~33 ℃, and macerating the system time is 120~168h.
The fibre percentage that hemp warm water comes unstuck is lower with respect to technology of the present invention: adopt the present invention can make the hemp fibre percentage improve 1.5%~2%, wherein fibre percentage is meant the weight shared former scapus heavy ratio of the hemp scapus of constant weight through the resulting hemp of system fiber crops processing.
The hemp warm water hemp intensity of producing of coming unstuck is low: adopting natural Degumming method fibre strength that big anesthesia stem produces is 170~180N.
The hemp warm water energy consumption of coming unstuck is bigger: hemp degumming production need make the temperature in the retted fibre pond reach certain temperature, realize that this condition factory need adopt steam to mend temperature, like this every day each pond to mend the temperature once need to expend a large amount of steam, promptly to expend a large amount of fire coals.Add the method that the bacterium degumming baths carries out hemp degumming and have the big problem of quantity of wastewater effluent of macerating in the system and adopt.
Summary of the invention
The present invention be for the method that solves existing hemp degumming exist the cycle of coming unstuck long, fibre percentage is low, hemp intensity is low, macerate the big problem of quantity of wastewater effluent in the system, and provides hemp to add the method for bacterium degumming baths recycling carrying out hemp degumming.
Hemp adds bacterium degumming baths recycling and carries out the method for hemp degumming and realize according to the following steps: one, hemp degumming is with the activation of bacterial classification: colon bacillus HDDMG05 and bacillus licheniformis HDDMM02 are cultivated 24h respectively on beef-protein medium under 30 ℃; Two, first order seed is cultivated: with oese respectively the bacterial classification of picking step 1 activation access in 200~500mL industry enlarged culture base, under 28~32 ℃ of conditions, cultivate 20~30h; Three, secondary seed is cultivated: the bacterium liquid after first order seed is cultivated by volume mark is that 0.5%~2% inoculum concentration is linked in 200~500L industry enlarged culture base, cultivates 20~30h under 28~32 ℃ of conditions; Four, three grades of seed culture: the bacterium liquid after secondary seed cultivated by volume mark is that 0.5%~2% inoculum concentration is linked in 2~5t industry enlarged culture base, cultivates 20~30h under 28~32 ℃ of conditions; Five, in the hemp degumming pond, add the big anesthesia stem of 4~6t, 2~2.5 kilograms sodium chloride and 3~5 kilograms urea, then the bacterium liquid after three grades of seed culture is linked in the hemp degumming pond with 1% of big anesthesia stem weight, fill with the hemp degumming pond with clear water again, heat to 30 ℃~33 ℃ and macerate the system hemp, adopt the method for putting forth to judge to arrive to finish when macerating the system terminal point to adopt to add the bacterium degumming baths and carry out hemp degumming; Adding of producing when six, the system terminal point is macerated in arrival in the step 5 joins in the new hemp degumming pond after the bacterium degumming baths reclaims, add the big anesthesia stem of 4~6t, 2~2.5 kilograms sodium chloride and 3~5 kilograms urea again, fill with the hemp degumming pond with clear water again, heat to 30 ℃~33 ℃ and macerate the system hemp, adopt the method for putting forth to judge to arrive and finish hemp when macerating the system terminal point and add bacterium degumming baths recycling carrying out hemp degumming;
Wherein the HDDMG05 of colon bacillus described in the step 1 is preserved in Chinese typical culture collection center, deposit number is CCTCC No:M 208082, preservation date is on June 2nd, 2008, and patent name is " a kind of engineered strain that produces pectase ", and publication number is CN 101386825A;
The HDDMM02 of bacillus licheniformis described in the step 1 is preserved in Chinese typical culture collection center, deposit number is CCTCC No:M 208081, preservation date is on June 2nd, 2008, and patent name is " a kind of engineered strain that produces mannase ", and publication number is CN 101386824A;
Industrial enlarged culture base is the glucose by 10g in the step 2, the soybean diffusion juice of 10mL, and the macrogranule salt of 5g, the urea of 2g add water and are settled to 1000mL, and pH 7.0~7.2, and 30min sterilizes under 112 ℃ of conditions;
Add the addition that joins in the hemp degumming pond after the bacterium degumming baths reclaims in the step 6 and account for 50% of hemp degumming pool volume.
The present invention carries out hemp degumming and arrives the bacterium degumming baths that adds that produces when macerating the system terminal point and carry out recycling adding the bacterium degumming baths, all reduce by 50% discharge of wastewater (adding the discharge of wastewater that the bacterium degumming baths carries out hemp degumming with employing relatively) every day, save hemp degumming energy consumption 10%~15%; Simultaneously the cycle of coming unstuck of big anesthesia stem shortens to that 80~120h, hemp fibre percentage have improved 3%~5%, hemp intensity is brought up to 220~280N, has improved the hemp quality.
The specific embodiment
Technical solution of the present invention is not limited to the following cited specific embodiment, also comprises any combination between each specific embodiment.
The specific embodiment one: the present embodiment hemp adds bacterium degumming baths recycling and carries out the method for hemp degumming and realize according to the following steps: one, hemp degumming is with the activation of bacterial classification: colon bacillus HDDMG05 and bacillus licheniformis HDDMM02 are cultivated 24h respectively on beef-protein medium under 30 ℃; Two, first order seed is cultivated: with oese respectively the bacterial classification of picking step 1 activation access in 200~500mL industry enlarged culture base, under 28~32 ℃ of conditions, cultivate 20~30h; Three, secondary seed is cultivated: the bacterium liquid after first order seed is cultivated by volume mark is that 0.5%~2% inoculum concentration is linked in 200~500L industry enlarged culture base, cultivates 20~30h under 28~32 ℃ of conditions; Four, three grades of seed culture: the bacterium liquid after secondary seed cultivated by volume mark is that 0.5%~2% inoculum concentration is linked in 2~5t industry enlarged culture base, cultivates 20~30h under 28~32 ℃ of conditions; Five, in the hemp degumming pond, add the big anesthesia stem of 4~6t, 2~2.5 kilograms sodium chloride and 3~5 kilograms urea, then the bacterium liquid after three grades of seed culture is linked in the hemp degumming pond with 1% of big anesthesia stem weight, fill with the hemp degumming pond with clear water again, heat to 30 ℃~33 ℃ and macerate the system hemp, adopt the method for putting forth to judge to arrive to finish when macerating the system terminal point to adopt to add the bacterium degumming baths and carry out hemp degumming; Adding of producing when six, the system terminal point is macerated in arrival in the step 5 joins in the new hemp degumming pond after the bacterium degumming baths reclaims, add the big anesthesia stem of 4~6t, 2~2.5 kilograms sodium chloride and 3~5 kilograms urea again, fill with the hemp degumming pond with clear water again, heat to 30 ℃~33 ℃ and macerate the system hemp, adopt the method for putting forth to judge to arrive and finish hemp when macerating the system terminal point and add bacterium degumming baths recycling carrying out hemp degumming;
Wherein the HDDMG05 of colon bacillus described in the step 1 is preserved in Chinese typical culture collection center, deposit number is CCTCC No:M 208082, preservation date is on June 2nd, 2008, and patent name is " a kind of engineered strain that produces pectase ", and publication number is CN 101386825A;
The HDDMM02 of bacillus licheniformis described in the step 1 is preserved in Chinese typical culture collection center, deposit number is CCTCC No:M 208081, preservation date is on June 2nd, 2008, and patent name is " a kind of engineered strain that produces mannase ", and publication number is CN 101386824A;
Industrial enlarged culture base is the glucose by 10g in the step 2, the soybean diffusion juice of 10mL, and the macrogranule salt of 5g, the urea of 2g add water and are settled to 1000mL, and pH 7.0~7.2, and 30min sterilizes under 112 ℃ of conditions;
Add the addition that joins in the hemp degumming pond after the bacterium degumming baths reclaims in the step 6 and account for 50% of hemp degumming pool volume.
Beef-protein medium is by the beef extract of 3g in the present embodiment step 1, the peptone of 10g, and the NaCl of 5g, the agar of 15g~20g adds water and is settled to 1000mL, and pH 7.0~7.2, and 30min sterilizes under 121 ℃ of conditions.
The specific embodiment two: present embodiment and the specific embodiment one are different be in the step 2 with oese respectively the bacterial classification of picking step 1 activation access in 250~400mL industry enlarged culture base, under 30 ℃ of conditions, cultivate 24h.Other steps are identical with the specific embodiment one with parameter.
The specific embodiment three: present embodiment and the specific embodiment one are different be after in the step 3 first order seed being cultivated bacterium liquid by volume mark be that 1%~1.5% inoculum concentration is linked in 250~400L industry enlarged culture base, under 30 ℃ of conditions, cultivate 24h.Other steps are identical with the specific embodiment one with parameter.
The specific embodiment four: present embodiment and the specific embodiment one are different be after in the step 4 secondary seed being cultivated bacterium liquid by volume mark be that 1%~1.5% inoculum concentration is linked in 2.5~4t industry enlarged culture base, under 30 ℃ of conditions, cultivate 24h.Other steps are identical with the specific embodiment one with parameter.
The specific embodiment five: to be step 6 join in the new hemp degumming pond after the bacterium degumming baths reclaims arriving adding of producing when macerating the system terminal point in the step 5 for present embodiment and the specific embodiment one different, add the big anesthesia stem of 4t, 2 kilograms sodium chloride and 3 kilograms urea again, fill with the hemp degumming pond with clear water again.Other is identical with the specific embodiment one.
The specific embodiment six: to be step 6 join in the new hemp degumming pond after the bacterium degumming baths reclaims arriving adding of producing when macerating the system terminal point in the step 5 for present embodiment and the specific embodiment one different, add the big anesthesia stem of 6t, 2.5 kilograms sodium chloride and 5 kilograms urea again, fill with the hemp degumming pond with clear water again.Other is identical with the specific embodiment one.
The specific embodiment seven: to be step 6 join in the new hemp degumming pond after the bacterium degumming baths reclaims arriving adding of producing when macerating the system terminal point in the step 5 for present embodiment and the specific embodiment one different, add the big anesthesia stem of 4.5~5.5t, 2.1~2.4 kilograms sodium chloride and 3.5~4.5 kilograms urea again, fill with the hemp degumming pond with clear water again.Other is identical with the specific embodiment one.
The specific embodiment eight: to be step 6 join in the new hemp degumming pond after the bacterium degumming baths reclaims arriving adding of producing when macerating the system terminal point in the step 5 for present embodiment and the specific embodiment one different, add the big anesthesia stem of 5t, 2.3 kilograms sodium chloride and 4 kilograms urea again, fill with the hemp degumming pond with clear water again.Other is identical with the specific embodiment one.
The specific embodiment nine: the present embodiment hemp adds bacterium degumming baths recycling and carries out the method for hemp degumming and realize according to the following steps: one, hemp degumming is with the activation of bacterial classification: colon bacillus HDDMG05 and bacillus licheniformis HDDMM02 are cultivated 24h respectively on beef-protein medium under 30 ℃; Two, first order seed is cultivated: with oese respectively the bacterial classification of picking step 1 activation access in the 300mL industry enlarged culture base, under 30 ℃ of conditions, cultivate 24h; Three, secondary seed is cultivated: the bacterium liquid after first order seed is cultivated by volume mark is that 1% inoculum concentration is linked in the 300L industry enlarged culture base, cultivates 24h under 30 ℃ of conditions; Four, three grades of seed culture: the bacterium liquid after secondary seed cultivated by volume mark is that 1% inoculum concentration is linked in the 3t industry enlarged culture base, cultivates 24h under 30 ℃ of conditions; Five, the big anesthesia stem, 2.3 kilograms sodium chloride and 4 kilograms the urea that in the hemp degumming pond, add 5t, then the bacterium liquid after three grades of seed culture is linked in the hemp degumming pond with 1% of big anesthesia stem weight, fill with the hemp degumming pond with clear water again, heat to 32 ℃ and macerate the system hemp, adopt the method for putting forth to judge to arrive to finish when macerating the system terminal point to adopt to add the bacterium degumming baths and carry out hemp degumming; Adding of producing when six, the system terminal point is macerated in arrival in the step 5 joins in the new hemp degumming pond after the bacterium degumming baths reclaims, add the big anesthesia stem of 5t, 2.3 kilograms sodium chloride and 4 kilograms urea again, fill with the hemp degumming pond with clear water again, heat to 32 ℃ and macerate the system hemp, adopt the method for putting forth to judge to arrive and finish hemp when macerating the system terminal point and add bacterium degumming baths recycling carrying out hemp degumming;
Wherein the HDDMG05 of colon bacillus described in the step 1 is preserved in Chinese typical culture collection center, deposit number is CCTCC No:M 208082, preservation date is on June 2nd, 2008, and patent name is " a kind of engineered strain that produces pectase ", and publication number is CN 101386825A;
The HDDMM02 of bacillus licheniformis described in the step 1 is preserved in Chinese typical culture collection center, deposit number is CCTCC No:M 208081, preservation date is on June 2nd, 2008, and patent name is " a kind of engineered strain that produces mannase ", and publication number is CN 101386824A;
Industrial enlarged culture base is the glucose by 10g in the step 2, the soybean diffusion juice of 10mL, and the macrogranule salt of 5g, the urea of 2g add water and are settled to 1000mL, and pH 7.0~7.2, and 30min sterilizes under 112 ℃ of conditions;
Add the addition that joins in the hemp degumming pond after the bacterium degumming baths reclaims in the step 6 and account for 50% of hemp degumming pool volume.
Carry out hemp degumming and arrive the bacterium degumming baths that adds that produces when macerating the system terminal point and carry out recycling adding the bacterium degumming baths in the present embodiment, after testing, present embodiment all reduces 50% discharge of wastewater (relatively with adopt add the discharge of wastewater that the bacterium degumming baths carries out hemp degumming) every day, saves hemp degumming with energy consumption 15%; Simultaneously the cycle of coming unstuck of big anesthesia stem shortens to that 80h, hemp fibre percentage have improved 5%, hemp intensity is brought up to 280N, has improved the hemp quality.

Claims (6)

1. hemp adds the method that hemp degumming is carried out in bacterium degumming baths recycling, it is characterized in that hemp adds that the method for bacterium degumming baths recycling carrying out hemp degumming realizes according to the following steps: one, hemp degumming is with the activation of bacterial classification: colon bacillus HDDMG05 and bacillus licheniformis HDDMM02 are cultivated 24h respectively on beef-protein medium under 30 ℃; Two, first order seed is cultivated: with oese respectively the bacterial classification of picking step 1 activation access in 200~500mL industry enlarged culture base, under 28~32 ℃ of conditions, cultivate 20~30h; Three, secondary seed is cultivated: the bacterium liquid after first order seed is cultivated by volume mark is that 0.5%~2% inoculum concentration is linked in 200~500L industry enlarged culture base, cultivates 20~30h under 28~32 ℃ of conditions; Four, three grades of seed culture: the bacterium liquid after secondary seed cultivated by volume mark is that 0.5%~2% inoculum concentration is linked in 2~5t industry enlarged culture base, cultivates 20~30h under 28~32 ℃ of conditions; Five, in the hemp degumming pond, add the big anesthesia stem of 4~6t, 2~2.5 kilograms sodium chloride and 3~5 kilograms urea, then the bacterium liquid after three grades of seed culture is linked in the hemp degumming pond with 1% of big anesthesia stem weight, fill with the hemp degumming pond with clear water again, heat to 30 ℃~33 ℃ and macerate the system hemp, adopt the method for putting forth to judge to arrive to finish when macerating the system terminal point to adopt to add the bacterium degumming baths and carry out hemp degumming; Adding of producing when six, the system terminal point is macerated in arrival in the step 5 joins in the new hemp degumming pond after the bacterium degumming baths reclaims, add the big anesthesia stem of 4~6t, 2~2.5 kilograms sodium chloride and 3~5 kilograms urea again, fill with the hemp degumming pond with clear water again, heat to 30 ℃~33 ℃ and macerate the system hemp, adopt the method for putting forth to judge to arrive and finish hemp when macerating the system terminal point and add bacterium degumming baths recycling carrying out hemp degumming;
Wherein the HDDMG05 of colon bacillus described in the step 1 is preserved in Chinese typical culture collection center, deposit number is CCTCC No:M 208082, preservation date is on June 2nd, 2008, and patent name is " a kind of engineered strain that produces pectase ", and publication number is CN 101386825A;
The HDDMM02 of bacillus licheniformis described in the step 1 is preserved in Chinese typical culture collection center, deposit number is CCTCC No:M 208081, preservation date is on June 2nd, 2008, and patent name is " a kind of engineered strain that produces mannase ", and publication number is CN 101386824A;
Industrial enlarged culture base is the glucose by 10g in the step 2, the soybean diffusion juice of 10mL, and the macrogranule salt of 5g, the urea of 2g add water and are settled to 1000mL, and pH 7.0~7.2, and 30min sterilizes under 112 ℃ of conditions;
Add the addition that joins in the hemp degumming pond after the bacterium degumming baths reclaims in the step 6 and account for 50% of hemp degumming pool volume.
2. hemp according to claim 1 adds the method for bacterium degumming baths recycling carrying out hemp degumming, it is characterized in that in the step 2 with oese respectively the bacterial classification of picking step 1 activation access in 250~400mL industry enlarged culture base, under 30 ℃ of conditions, cultivate 24h.
3. hemp according to claim 1 adds the method for bacterium degumming baths recycling carrying out hemp degumming, bacterium liquid after it is characterized in that in the step 3 first order seed cultivated by volume mark is that 1%~1.5% inoculum concentration is linked in 250~400L industry enlarged culture base, cultivates 24h under 30 ℃ of conditions.
4. hemp according to claim 1 adds the method for bacterium degumming baths recycling carrying out hemp degumming, bacterium liquid after it is characterized in that in the step 4 secondary seed cultivated by volume mark is that 1%~1.5% inoculum concentration is linked in 2.5~4t industry enlarged culture base, cultivates 24h under 30 ℃ of conditions.
5. hemp according to claim 1 adds the method for bacterium degumming baths recycling carrying out hemp degumming, it is characterized in that step 6 joins in the new hemp degumming pond after the bacterium degumming baths reclaims arriving adding of producing when macerating the system terminal point in the step 5, add the big anesthesia stem of 4.5~5.5t, 2.1~2.4 kilograms sodium chloride and 3.5~4.5 kilograms urea again, fill with the hemp degumming pond with clear water again.
6. hemp according to claim 1 adds the method for bacterium degumming baths recycling carrying out hemp degumming, it is characterized in that step 6 joins in the new hemp degumming pond after the bacterium degumming baths reclaims arriving adding of producing when macerating the system terminal point in the step 5, add the big anesthesia stem of 5t, 2.3 kilograms sodium chloride and 4 kilograms urea again, fill with the hemp degumming pond with clear water again.
CN2010106114296A 2010-12-29 2010-12-29 Method for recycling hemp strain-containing degumming liquid for degumming hemp Expired - Fee Related CN102174715B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103194804A (en) * 2013-03-12 2013-07-10 江西恩达麻世纪科技股份有限公司 Energy-saving environment-friendly biological degumming technology
CN110387585A (en) * 2019-08-19 2019-10-29 东嘉麻棉(常州)有限公司 Flax fiber microbial degumming technique

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1594675A (en) * 2004-07-16 2005-03-16 黑龙江大学 Microorganism rapid degumming and warm water retting method for flax
CN1594676A (en) * 2004-07-16 2005-03-16 黑龙江大学 Bacteria added retting liquid recycling method in warm water retting
CN101285211A (en) * 2008-06-05 2008-10-15 黑龙江省科学院亚麻综合利用研究所 Microorganism retting flax method for crudefiber crops

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1594675A (en) * 2004-07-16 2005-03-16 黑龙江大学 Microorganism rapid degumming and warm water retting method for flax
CN1594676A (en) * 2004-07-16 2005-03-16 黑龙江大学 Bacteria added retting liquid recycling method in warm water retting
CN101285211A (en) * 2008-06-05 2008-10-15 黑龙江省科学院亚麻综合利用研究所 Microorganism retting flax method for crudefiber crops

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103194804A (en) * 2013-03-12 2013-07-10 江西恩达麻世纪科技股份有限公司 Energy-saving environment-friendly biological degumming technology
CN103194804B (en) * 2013-03-12 2015-05-27 江西恩达麻世纪科技股份有限公司 Energy-saving environment-friendly biological degumming technology
CN110387585A (en) * 2019-08-19 2019-10-29 东嘉麻棉(常州)有限公司 Flax fiber microbial degumming technique

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