CN102174594A - Efficient enzyme hydrolysis method of lignocellulose biomass - Google Patents
Efficient enzyme hydrolysis method of lignocellulose biomass Download PDFInfo
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Abstract
The invention discloses an efficient enzyme hydrolysis method of lignocellulose biomass, comprising the following steps: pretreating the lignocellulose biomass through high-temperature aqueous water to obtain a lignocellulose raw material to be used as a substrate; dispersing the substrate into an acetic acid-sodium acetate buffer solution in a certain concentration; adding a nonionic surfactant and cellulose; stirring the materials; hydrolyzing for some time; adding a certain amount of materials twice; and simultaneously adding the nonionic surfactant and the cellulose; and continuously hydrolyzing to obtain a saccharide compound and lignocellulose residues. In the method provided by the invention, high-temperature aqueous water pretreatment, batch charging, nonionic surfactant adding and stirring and an enzyme hydrolysis process are coupled, thus the hydrolysis efficiency is high, the concentration of the enzymolysized lignocellulose raw material can be greatly improved; the concentration reaches 30%; the sugar generation concentration and the hydrolysis efficiency are high under the condition of high-concentration lignocellulose raw materials; the enzymolysized raw material concentration is improved; and simultaneously the high-concentration sugar is produced, thus improving the hydrolysis efficiency, and realizing the efficient enzyme hydrolysis of the lignocellulose biomass.
Description
Technical field:
The invention belongs to the lignocellulose technology for hydrolyzing of biological chemistry and chemical field, be specifically related to the method for the efficient enzymic hydrolysis of a kind of lignocellulose-like biomass.
Background technology:
Along with the high development of society, the consumption of fossil oil is increasing, because fossil oil is non-renewable, seeking substitute energy becomes the focus that people pay close attention to.Alcohol fuel is owing to have high octane value, cleaning and low CO
2The discharging and gain great popularity.Preparation alcoholic acid traditional raw material comes from grain and sugar crops such as corn, wheat, sugarcane, the production technology maturation, but have the problem of " strive grain, strive ground " with farming with the people.Utilize lignocellulose-like biomass such as agriculture and forestry organic waste material such as various crop materials, wood chip, branch such as maize straw, corn cob, paddy rice stalk to prepare cellulosic ethanol, be known as " s-generation alcohol fuel ", it is in the added value that improves agriculture and forestry organic waste material, reduction environmental pollution, and aspects such as promotion rural economic development are significant.
The mainstream technology of cellulosic ethanol preparation comprises pre-treatment, enzymic hydrolysis and fermentation.This is because lignocellulose-like biomass mainly is made up of Mierocrystalline cellulose, hemicellulose and xylogen three big components, connect the structure that forms densification by chemical bond between each component, wherein Mierocrystalline cellulose and hemicellulose are the glycosyl high molecular polymers, with it by pre-treatment and cellulase hydrolysis, obtain the carbohydrate product in hemicellulose and the Mierocrystalline cellulose, can be with its preparing ethanol by fermentation.Efficiently obtaining the carbohydrate product in the lignocellulose-like biomass, is the technological difficulties of most critical in the cellulose ethanol technology.
Obtaining of carbohydrate product mainly from pre-treatment and enzymic hydrolysis.Pretreated purpose is to reduce the content of xylogen and hemicellulose, reduces cellulosic degree of crystallinity, increase cellulase to pre-treatment after the accessibility of cellulosic material, obtain the sugar in the hemicellulose simultaneously; Enzymic hydrolysis is with cellulase pretreated cellulosic material to be hydrolyzed into glucose.The main problem of preconditioning technique existence at present is that chemical process (acid, alkali and organic solvent etc.) process often exists pollution, and biological process pre-treatment (utilizing lignin degradings such as whiterot fungi) inefficiency causes pre-treatment cost height; The cellulase cost of using in the enzymic hydrolysis is higher, and the carbohydrate production concentration of generation is lower, causes enzymic hydrolysis efficient lower.
Compare with other pretreatment modes, the high-temperature liquid state water treatment belongs to physical method, be described as green pretreatment technology, except water, need not to add chemical reagent in its treating processes, reduced pollution, produce more a spot of fermentation inhibitor environment, can have a negative impact to fermentation hardly, most hemicellulose can be converted into the carbohydrate product, can remove the part xylogen, promote the accessibility of the enzyme of cellulosic material.But this pretreatment process can not be removed xylogen in a large number, causes the pre-treatment material enzymolysis efficiency of high density low, and it is not high to produce sugared concentration.
Raw material with high density carries out enzymic hydrolysis, to the carbohydrate production concentration that improves output is unusual effective means, but when pretreated lignocellulose solid material concentration is higher, must bring viscosity big, mix uneven problem, also can the inhibitory enzyme hydrolysis carry out smoothly.
Carry out the patent inquiry by the State Intellectual Property Office of the People's Republic of China website, the relevant patent of enzymolysis is as follows again after pre-treatment at present domestic relevant lignocellulose-like biomass:
(1), a kind of technology of efficiently preprocessing lignocellulose, open (bulletin) number: CN101736631A;
(2), cellulose materials is changed into the alcoholic acid method and apparatus, open (bulletin) number: CN101268121A;
(3), a kind of method of high-efficiency pretreatment on biomass, open (bulletin) number: CN101586136;
(4), a kind of method of efficiently saccharifying bagasse, open (bulletin) number: CN101619332A;
(5), a kind of effectively hydrolyzing lignocellulosic biolobic material prepares the method for polycomponent liquid glucose and xylogen simultaneously, open (bulletin) number: CN101285106A;
(6), a kind of method that makes the plain efficiently saccharifying of straw pulp fiber, open (bulletin) number: CN101613728A;
Patent (1)～(3) are the relevant high temperature liquid water preprocessing lignocellulose patents of hydrolysis again.Patent (1) has been taked two step pre-treatment to lignocellulose, and high temperature liquid water is the first step wherein, and second step was that diluted acid is handled, and this is different from the pre-treatment of the present invention to lignocellulose.Patent (2) is essentially different to processing mode and the present invention of pretreated lignocellulose though also taked the high-temperature liquid state water pretreatment.Patent (3) has certain dependency with the present invention, but its special emphasis is to propose a kind of new pretreatment technology, and promptly two step segmentation alternating temperature high temperature liquid water hydrolysis processs do not relate to follow-up enzymic hydrolysis.The pretreatment process that adopt patent (4)～(6) is alkaline purification or acid treatment, and this and the present invention are essentially different.Certainly, the relevant patent of relevant enzymic hydrolysis lignocellulose more than these.Because there are difference in essence in pretreatment process that they are taked and enzymatic hydrolysis process and the present invention, therefore do not enumerate one by one.
Summary of the invention:
The purpose of this invention is to provide the method that a kind of simple, environmental protection, enzymic hydrolysis are handled the material concentration height, produced the high efficient enzymic hydrolysis of lignocellulose-like biomass of sugared concentration height and hydrolysis efficiency.
After the present invention adopts the high temperature liquid water pretreatment process that lignocellulose-like biomass is carried out pre-treatment, obtain lignocellulosic material, be scattered in acetate-sodium acetate buffer with finite concentration, add a certain proportion of nonionogenic tenside and cellulase again, the mixing of materials mode of taking to stir is after hydrolysis for some time, divide secondary in reaction system, to add a certain amount of material again, add simultaneously nonionogenic tenside and cellulase by a certain percentage and continue hydrolysis, obtain mainly to come from cellulosic saccharide compound and lignocellulose residue, improve the enzymolysis material concentration simultaneously thereby be implemented in, the output high concentration sugar, the purpose of raising enzymolysis efficiency.
The method of the efficient enzymic hydrolysis of lignocellulose-like biomass of the present invention is characterized in that, may further comprise the steps:
In pH4.5～4.8, temperature is under 45～55 ℃ the condition, originally with the quality volume fraction ratio of 10%～15%g/mL, the lignocellulosic material that lignocellulose-like biomass is obtained after the high-temperature liquid state water pretreatment, be added in the buffered soln of 0.05～0.1M acetate-sodium acetate, add cellulase by the cellulosic amount of 10～20FPU/g, ratio by 0.075～0.2mL/g lignocellulosic material adds nonionogenic tenside, after the hydrolysis 12～24 hours, press half adding lignocellulosic material of initial add-on again, add cellulase by the cellulosic amount of 10～20FPU/g simultaneously, ratio by 0.075～0.2mL/g lignocellulosic material adds nonionogenic tenside, after the hydrolysis 36～48 hours, add lignocellulosic material again, making the total quality volume fraction of the lignocellulosic material of three addings is 20%～30%g/ml, add cellulase by the cellulosic amount of 10～20FPU/g simultaneously, ratio by 0.075～0.2mL/g lignocellulosic material adds nonionogenic tenside, continue hydrolysis to 108～120 hour, the mode mixing material of whole hydrolytic process to stir.
The method of the efficient enzymic hydrolysis of described lignocellulose-like biomass, preferred steps is: at pH4.8, temperature is under 50 ℃ the condition, originally with the quality volume fraction ratio of 10%g/mL, the lignocellulosic material that lignocellulose-like biomass is obtained after the high-temperature liquid state water pretreatment, be added in the buffered soln of 0.05M acetate-sodium acetate, press the cellulosic amount of 20FPU/g and add cellulase, the ratio of pressing the 0.075mL/g lignocellulosic material adds Tween 80, after the hydrolysis 24 hours, press half adding lignocellulosic material of initial add-on again, press the cellulosic amount of 20FPU/g and add cellulase, the ratio of pressing the 0.075mL/g lignocellulosic material adds Tween 80, after the hydrolysis 48 hours, add lignocellulosic material again, making the total quality volume fraction of the lignocellulosic material of three addings is 20%g/ml, press the cellulosic amount of 20FPU/g and add cellulase, the ratio of pressing the 0.075mL/g lignocellulosic material adds Tween 80, continue hydrolysis to 120 hour, the mode mixing material of whole hydrolytic process to stir.
The described lignocellulosic material that lignocellulose-like biomass is obtained after the high-temperature liquid state water pretreatment, preferred steps is: lignocellulose-like biomass is crushed to 40～60 orders, is the ratio of 1: 10～20g/mL by material butt solid-to-liquid ratio, adds water in reactive system, reaction conditions is: 2～4MPa, 170～190 ℃, 300～500rpm, 15～30min, after processing finishes, solid-liquid separation washes the pre-treatment residue, obtains lignocellulosic material.
Described lignocellulose-like biomass preferably includes agriculture and forestry organic waste material such as sorgo stalk slag, bagasse, maize straw, corn cob, grass stalk and wood chip and speed, and to give birth to main component such as energy forest be the lignocellulose-like biomass of hemicellulose, Mierocrystalline cellulose and xylogen.
Described nonionogenic tenside is preferably Tween 80.Tween 80 can not pollute environment, and harmless to microorganism.
Alr mode can be magnetic agitation or mechanical stirring.With the better mixing material of the mode that stirs, increase the reaction probability of enzyme and substrate, thus the raising enzymolysis efficiency.
Beneficial effect of the present invention is as follows:
Advantages such as that the high-temperature liquid state water pretreatment has is inexpensive, environmental protection, easy handling need not pretreated residue is carried out detoxification treatment, simplify technology, save cost.The nonionogenic tenside that adds in the enzymic hydrolysis process, can significantly improve the material enzymolysis efficiency, this is because nonionogenic tenside can combine with xylogen by hydrophobic interaction, cellulase binding site on the shielding xylogen reduces the ineffective adsorption of xylogen to cellulase, increases the amount of resolvase, thereby promote the cellulase hydrolysis reaction process, improve enzymolysis efficiency, and help reducing the consumption of enzyme, thereby reduce the enzyme digestion reaction cost.In the enzymic hydrolysis process, take 3 batches of branches, a certain amount of lignocellulosic material of each interpolation, add a certain amount of cellulase and nonionogenic tenside, the hydrolysis regular hour, optimize enzymatic hydrolysis condition, thereby effectively reduced the viscosity of enzymolysis material, improve sugar yield, thereby improved hydrolysis efficiency.
The present invention is coupled the enzyme hydrolysis process in batches under high-temperature liquid state water pretreatment and the specified conditions, the hydrolysis efficiency height, and improved the lignocellulosic material concentration that enzymic hydrolysis is handled greatly, concentration can reach 30%g/ml (quality volume fraction), under the situation of the lignocellulosic material of high density, through technology of the present invention, it produces sugared concentration height, hydrolysis efficiency is also high, thereby successful solution big in the caused viscosity of the lignocellulosic material of high density, mix inhomogeneous, the carrying out smoothly of inhibitory enzyme hydrolysis, and cause the sugared concentration of product low, the difficult problem that hydrolysis efficiency is low is implemented in and improves the enzymolysis material concentration simultaneously, the output high concentration sugar, improve the purpose of enzymolysis efficiency, thereby realized the efficient enzymic hydrolysis of lignocellulose-like biomass.
Description of drawings:
Fig. 1 is a process flow sheet of the present invention.
Embodiment:
Following examples are to further elaboration of the present invention, rather than limitation of the present invention.
As shown in Figure 1, lignocellulose-like biomass is removed impurity such as dust and sandstone, be crushed to 40～60 orders, by material butt solid-to-liquid ratio is the ratio of 1: 10～20g/mL, add deionized water in reactive system, reaction conditions is: 2～4MPa, 170～190 ℃, 300～500rpm, 15～30min, after handling, solid-liquid separation obtains hemicellulose hydrolysate and pre-treatment residue, and the pre-treatment residue is washed with water totally, purpose is to remove the inhibition that a spot of furfural, acetate etc. influence enzymolysis and fermentation, obtains the raw material-lignocellulosic material of enzymic hydrolysis.This raw material is joined in the enzymic hydrolysis jar by following operation, in pH4.5～4.8, temperature is under 45～55 ℃ the condition, originally with the quality volume fraction ratio of 10%～15%g/mL, with lignocellulosic material, be added in the buffered soln of 0.05～0.1M acetate-sodium acetate, by 10～20FPU/g Mierocrystalline cellulose is the amount adding cellulase of dextran, ratio by 0.075～0.2mL/g lignocellulosic material adds nonionogenic tenside, with the stirring velocity hydrolysis of 10～200rpm after 12～24 hours, half adding lignocellulosic material of pressing initial add-on again is to enzymatic vessel, add cellulase by the cellulosic amount of 10～20FPU/g simultaneously, ratio by 0.075～0.2mL/g lignocellulosic material adds nonionogenic tenside, under the same reaction conditions, after the hydrolysis 36～48 hours, add lignocellulosic material again, making the total quality volume fraction of the lignocellulosic material of three addings is 20%～30%g/mL, add cellulase by the cellulosic amount of 10～20FPU/g, ratio by 0.075～0.2mL/g lignocellulosic material adds nonionogenic tenside, under identical reaction conditions, continue hydrolysis to 108～120 hour, the mode mixing material of whole hydrolytic process to stir, after enzymolysis finishes, solid-liquid separation obtains mainly to come from cellulosic carbohydrate product and lignin residue.
Comparative Examples 1
Get be crushed to 60 purposes squeeze the juice the back sugar-free sorgo stalk slag 2g, add in 10mL 0.05M acetate-sodium acetate buffer (pH4.8), ratio with the 20FPU/g dextran adds cellulase, hydrolysis 120h vibrates under 50 ℃, measure total reducing sugars concentration with the DNS method, obtaining total reducing sugars concentration is 15.3mg/mL, and the total reducing sugar rate of recovery is 13.7%.
Embodiment 1
The sorgo stalk slag of the back sugar-free of squeezing the juice is crushed to 40 orders, ratio by solid-to-liquid ratio 1: 10g/mL, join in the high temperature liquid water reactor, under 2Mpa, 170 ℃, the high-temperature liquid state water surrounding of 300rpm, handle 15min, finish pre-treatment, solid-liquid separation obtains hydrolysis of hemicellulose product and pretreated sorgo stalk slag substrate.Clean with clear water, remove enzymic hydrolysis and fermentation inhibitor, as the enzymic hydrolysis raw material.Get 1g sorgo stalk slag substrate as the enzymic hydrolysis raw material, add in 10mL 0.05M acetate-sodium acetate buffer (pH4.5), with the 10FPU/g Mierocrystalline cellulose is the ratio adding cellulase of dextran, add 0.075mL Tween80 simultaneously, stir hydrolysis down after 12 hours at 45 ℃, add 0.5g sorgo stalk slag substrate again, ratio with the 10FPU/g dextran adds cellulase, add 0.0375mL Tween80 simultaneously, after stirring hydrolysis 36h, add the pretreated sorgo stalk slag of 0.5g substrate again, the ratio adding cellulase with the 10FPU/g dextran adds 0.0375mLTween80 simultaneously, be hydrolyzed into 108h, measure total reducing sugars concentration with the DNS method, obtaining total reducing sugars concentration is 52.1mg/mL, and the total reducing sugar rate of recovery is 30.4%.
Embodiment 2
Sorgo stalk slag after squeezing the juice is crushed to 60 orders, join in the high temperature liquid water reactor by solid-to-liquid ratio 1: 20g/mL, under 4Mpa, 190 ℃, the high-temperature liquid state water surrounding of 500rpm, handle 30min, finish pre-treatment, solid-liquid separation, obtain hydrolysis of hemicellulose product and pretreated sorgo stalk slag substrate, clean with clear water, remove enzymic hydrolysis and fermentation inhibitor, as the enzymic hydrolysis raw material.Get 1.5g as the enzymic hydrolysis raw material, add in 10mL 0.1M acetate-sodium acetate buffer (pH4.8), ratio with the 20FPU/g dextran adds cellulase, add 0.3mL Tween80 simultaneously, stir hydrolysis after 24 hours at 55 ℃ of lower magnetic forces, add the 0.75g substrate again, the ratio adding cellulase with the 20FPU/g dextran adds 0.15mLTween80 simultaneously, behind the magnetic agitation hydrolysis 48h, add 0.75g pre-treatment material again, the ratio adding cellulase with the 20FPU/g dextran adds 0.15mL Tween80 simultaneously, be hydrolyzed into 120h, measure total reducing sugars concentration with the DNS method, obtaining total reducing sugars concentration is 92.3mg/mL, and the total reducing sugar rate of recovery is 58.2%.
Embodiment 3
Sorgo stalk slag after squeezing the juice is crushed to 60 orders, join in the high temperature liquid water reactor by solid-to-liquid ratio 1: 20g/mL, under 4Mpa, 180 ℃, the high-temperature liquid state water surrounding of 400rpm, handle 20min, finish pre-treatment, solid-liquid separation obtains hydrolysis of hemicellulose product and pretreated sorgo stalk slag substrate, cleans with clear water, remove enzymic hydrolysis and fermentation inhibitor, as the enzymic hydrolysis raw material.Get 1g as the enzymic hydrolysis raw material, add 10mL 0.05M acetate-sodium acetate buffer (pH4.8), ratio with the 20FPU/g dextran adds cellulase, add 0.075mL/g Tween80 simultaneously, stir hydrolysis after 24 hours at 50 ℃ of lower magnetic forces, add 0.5g pre-treatment material again, ratio with the 20FPU/g dextran adds cellulase, add 0.0375mL Tween80 simultaneously, behind the magnetic agitation hydrolysis 48h, add the pretreated substrate of 0.5g again, the ratio adding cellulase with the 20FPU/g dextran adds 0.0375mL Tween80 simultaneously, be hydrolyzed into 120h, measure total reducing sugars concentration with the DNS method, obtaining total reducing sugars concentration is 120.2mg/mL, and the total reducing sugar rate of recovery is 89.3%.
Comparative Examples 2
Sorgo stalk slag after squeezing the juice is crushed to 60 orders, join in the high temperature liquid water reactor by solid-to-liquid ratio 1: 20g/mL, under 4Mpa, 180 ℃, the high-temperature liquid state water surrounding of 400rpm, handle 20min, finish pre-treatment, solid-liquid separation obtains hydrolysis of hemicellulose product and pretreated sorgo stalk slag substrate, cleans with clear water, remove enzymic hydrolysis and fermentation inhibitor, as the enzymic hydrolysis raw material.Get 2g as the enzymic hydrolysis raw material, add 10mL 0.05M acetate-sodium acetate buffer (pH4.8), ratio with the 20FPU/g dextran adds cellulase, add 0.15mL Tween80 simultaneously, under 50 ℃, carry out magnetic agitation hydrolysis 120h, measure total reducing sugars concentration with the DNS method, obtaining total reducing sugars concentration is 105.5mg/mL, and the total reducing sugar rate of recovery is 64.9%.
By embodiment 1-3 and Comparative Examples 1 as seen, raw material adopts batch charging to stir after the high-temperature liquid state water pretreatment and adds the nonionogenic tenside hydrolysis, no matter on the sugared production concentration and the sugared rate of recovery, all is higher than the only technology of usefulness cellulase hydrolysis.
By embodiment 3 and Comparative Examples 2 as seen, with same sorgo stalk slag substrate is raw material, through behind the treatment process of embodiments of the invention 3, with compare behind the treatment process of the Comparative Examples 2 of disposable hydrolysis, its total reducing sugars concentration and the total reducing sugar rate of recovery are greatly improved, and show that its hydrolysis efficiency also is greatly improved.
From above embodiment as can be seen, lignocellulose-like biomass is after the water pretreatment of process high-temperature liquid state, taking to add Tween80, alr mode and batch hydrolysis technology process combined is hydrolyzed, the concentration to 30% of enzymolysis raw material can be improved, and the total reducing sugars concentration and the total reducing sugar rate of recovery can be significantly improved.
Claims (6)
1. the method for the efficient enzymic hydrolysis of lignocellulose-like biomass is characterized in that, may further comprise the steps:
In pH4.5～4.8, temperature is under 45～55 ℃ the condition, originally with the quality volume fraction ratio of 10%～15%g/mL, the lignocellulosic material that lignocellulose-like biomass is obtained after the high-temperature liquid state water pretreatment, be added in the buffered soln of 0.05～0.1M acetate-sodium acetate, add cellulase by the cellulosic amount of 10～20FPU/g, ratio by 0.075～0.2mL/g lignocellulosic material adds nonionogenic tenside, after the hydrolysis 12～24 hours, press half adding lignocellulosic material of initial add-on again, add cellulase by the cellulosic amount of 10～20FPU/g simultaneously, ratio by 0.075～0.2mL/g lignocellulosic material adds nonionogenic tenside, after the hydrolysis 36～48 hours, add lignocellulosic material again, making the total quality volume fraction of the lignocellulosic material of three addings is 20%～30%g/ml, add cellulase by the cellulosic amount of 10～20FPU/g simultaneously, ratio by 0.075～0.2mL/g lignocellulosic material adds nonionogenic tenside, continue hydrolysis to 108～120 hour, the mode mixing material of whole hydrolytic process to stir.
2. the method for the efficient enzymic hydrolysis of lignocellulose-like biomass according to claim 1, it is characterized in that, the method of the efficient enzymic hydrolysis of described lignocellulose-like biomass, step is: at pH4.8, temperature is under 50 ℃ the condition, originally with the quality volume fraction ratio of 10%g/mL, the lignocellulosic material that lignocellulose-like biomass is obtained after the high-temperature liquid state water pretreatment, be added in the buffered soln of 0.05M acetate-sodium acetate, press the cellulosic amount of 20FPU/g and add cellulase, the ratio of pressing the 0.075mL/g lignocellulosic material adds Tween 80, after the hydrolysis 24 hours, press half adding lignocellulosic material of initial add-on again, press the cellulosic amount of 20FPU/g and add cellulase, the ratio of pressing the 0.075mL/g lignocellulosic material adds Tween 80, after the hydrolysis 48 hours, add lignocellulosic material again, making the total quality volume fraction of the lignocellulosic material of three addings is 20%g/ml, press the cellulosic amount of 20FPU/g and add cellulase, the ratio of pressing the 0.075mL/g lignocellulosic material adds Tween 80, continue hydrolysis to 120 hour, the mode mixing material of whole hydrolytic process to stir.
3. the method for the efficient enzymic hydrolysis of lignocellulose-like biomass according to claim 1 and 2, it is characterized in that, the described lignocellulosic material that lignocellulose-like biomass is obtained after the high-temperature liquid state water pretreatment, step is: lignocellulose-like biomass is crushed to 40～60 orders, by material butt solid-to-liquid ratio is the ratio of 1: 10～20g/mL, add water in reactive system, reaction conditions is: 2～4MPa, 170～190 ℃, 300～500rpm, 15～30min, after processing finishes, solid-liquid separation washes the pre-treatment residue, obtains lignocellulosic material.
4. the method for the efficient enzymic hydrolysis of lignocellulose-like biomass according to claim 1 and 2, it is characterized in that described lignocellulose-like biomass is selected from sorgo stalk slag, bagasse, maize straw, corn cob, grass stalk, wood chip and speed and gives birth to energy forest.
5. the method for the efficient enzymic hydrolysis of lignocellulose-like biomass according to claim 1 is characterized in that, described nonionogenic tenside is Tween 80.
6. the method for the efficient enzymic hydrolysis of lignocellulose-like biomass according to claim 1 is characterized in that described alr mode comprises magnetic agitation and mechanical stirring.
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Cited By (14)
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| CN102703520A (en) * | 2012-05-22 | 2012-10-03 | 中国科学院广州能源研究所 | Method for producing ethanol through mixed fermentation of bagasse hydrolyzate and molasses |
| CN103614418A (en) * | 2013-11-27 | 2014-03-05 | 屠纪民 | Method for producing fuel ethanol through synchronous saccharification and fermentation |
| CN103993053A (en) * | 2014-05-07 | 2014-08-20 | 中国科学院广州能源研究所 | Method for pretreatment of biomass by water-ammonia coupling |
| CN104263779A (en) * | 2014-09-11 | 2015-01-07 | 大连理工大学 | Method for preparing reducing sugar by promoting lignocellulase to hydrolyze by ultrasonic pre-treatment in pressurized hot water |
| CN104328141A (en) * | 2014-10-30 | 2015-02-04 | 宁夏乙征生物工程有限公司 | Method for treating antibiotic residues by enzymic method |
| CN105838756A (en) * | 2016-05-12 | 2016-08-10 | 中国科学院广州能源研究所 | Water-saving and emission-reduction technology for producing sugar through enzymolysis of lignocellulose treated with alkali |
| CN106414756A (en) * | 2014-06-05 | 2017-02-15 | 植物材料工业公司 | Process for producing, in particular ethanol, by sequenced enzymatic hydrolysis of cellulose and hemicelluloses of a lignocellulosic raw material |
| CN107217081A (en) * | 2017-08-03 | 2017-09-29 | 中国科学院青岛生物能源与过程研究所 | A kind of efficient enzyme hydrolysis process method of the multistage of lignocellulosic |
| CN107916280A (en) * | 2016-10-11 | 2018-04-17 | 中国科学院大连化学物理研究所 | A kind of lignocellulosic substep diastatic fermentation method using polyethylene glycol water as medium |
| CN109535322A (en) * | 2018-11-15 | 2019-03-29 | 江苏科技大学 | A kind of preparation method of hydrophobic oleophilic oil mulberry fibrous material |
| WO2020034395A1 (en) * | 2018-08-17 | 2020-02-20 | 中国科学院青岛生物能源与过程研究所 | Method for pretreating lignocellulosic biomass matched with saccharification of whole fibrosome |
| CN111676257A (en) * | 2020-06-16 | 2020-09-18 | 天津科技大学 | Method for improving efficiency of producing fermentable sugar by high-concentration enzymatic hydrolysis of broad-leaved wood cellulose |
| CN112458132A (en) * | 2020-11-17 | 2021-03-09 | 汕头大学 | Method for preparing multi-element antibacterial probiotic by using single raw material |
| CN114058605A (en) * | 2021-11-10 | 2022-02-18 | 湖北大学 | Method for improving specific activity of cellulase and application thereof |
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| CN103614418A (en) * | 2013-11-27 | 2014-03-05 | 屠纪民 | Method for producing fuel ethanol through synchronous saccharification and fermentation |
| CN103993053A (en) * | 2014-05-07 | 2014-08-20 | 中国科学院广州能源研究所 | Method for pretreatment of biomass by water-ammonia coupling |
| CN103993053B (en) * | 2014-05-07 | 2017-01-04 | 中国科学院广州能源研究所 | A kind of water and the method for ammonia coupling preprocessing biomass |
| CN106414756A (en) * | 2014-06-05 | 2017-02-15 | 植物材料工业公司 | Process for producing, in particular ethanol, by sequenced enzymatic hydrolysis of cellulose and hemicelluloses of a lignocellulosic raw material |
| CN104263779A (en) * | 2014-09-11 | 2015-01-07 | 大连理工大学 | Method for preparing reducing sugar by promoting lignocellulase to hydrolyze by ultrasonic pre-treatment in pressurized hot water |
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| CN105838756A (en) * | 2016-05-12 | 2016-08-10 | 中国科学院广州能源研究所 | Water-saving and emission-reduction technology for producing sugar through enzymolysis of lignocellulose treated with alkali |
| CN107916280A (en) * | 2016-10-11 | 2018-04-17 | 中国科学院大连化学物理研究所 | A kind of lignocellulosic substep diastatic fermentation method using polyethylene glycol water as medium |
| CN107217081A (en) * | 2017-08-03 | 2017-09-29 | 中国科学院青岛生物能源与过程研究所 | A kind of efficient enzyme hydrolysis process method of the multistage of lignocellulosic |
| WO2020034395A1 (en) * | 2018-08-17 | 2020-02-20 | 中国科学院青岛生物能源与过程研究所 | Method for pretreating lignocellulosic biomass matched with saccharification of whole fibrosome |
| CN109535322A (en) * | 2018-11-15 | 2019-03-29 | 江苏科技大学 | A kind of preparation method of hydrophobic oleophilic oil mulberry fibrous material |
| CN111676257A (en) * | 2020-06-16 | 2020-09-18 | 天津科技大学 | Method for improving efficiency of producing fermentable sugar by high-concentration enzymatic hydrolysis of broad-leaved wood cellulose |
| CN112458132A (en) * | 2020-11-17 | 2021-03-09 | 汕头大学 | Method for preparing multi-element antibacterial probiotic by using single raw material |
| CN114058605A (en) * | 2021-11-10 | 2022-02-18 | 湖北大学 | Method for improving specific activity of cellulase and application thereof |
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