CN102174498A - Silicate bacteria vector as well as preparation method and application thereof - Google Patents
Silicate bacteria vector as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a silicate bacteria vector as well as a preparation method and application thereof. The silicate bacteria vector comprises grass carbon and silicon-containing substances or comprises grass carbon, silicon-containing substances and bentonite. The silicon-containing substances are selected from monocalcium silicate, dicalcium silicate, calcium magnesium silicate, sodium metasilicate, steel cinder, coal ash water-quenching slag, large slag ash, yellow phosphorous cinder or calcium metasilicate minerals. Compared with the traditional silicate bacteria vector with single grass carbon, the silicate bacteria vector prepared in the invention can ensure that silicate bacteria fertilizer supplies effective silicon for crops at the beginning, and the defect of slow increase of effective silicon content in single silicate microbial inoculum is completely overcome.
Description
[technical field]
The invention belongs to agriculture technical field of microbe application.More specifically, the present invention relates to a kind of silicate bacteria carrier, also relate to the preparation method of described silicate bacteria carrier, also relate to the purposes of described silicate bacteria carrier.
[background technology]
Silicon is listed in the fourth-largest first fertilizer after nitrogen, phosphorus, potassium at international soil circle.The silicon element has the function of " three anti-, three short, two improvement ", i.e. three anti-promptly resistant to lodging, disease and insect resistances, degeneration-resistant to crop; Three urge promptly to promote photosynthesis, promotion root growth and development, the utilization of promotion nutrition absorption; Two improvement promptly improve crop quality, improve the soil.Siliceous fertilizer is mainly used in happiness silicon crops such as paddy rice, wheat, corn, and is the most responsive to silicon with paddy rice especially.
People are divided into three kinds with available silicon content in the soil: (1) soil available silicon content 100mg/kg with interior be decided to be extremely scarce; (2) the soil available silicon content is decided to be scarce slightly at 100～250mg/kg; (3) the soil available silicon content is not decided to be scarce above 250mg/kg.The distributed pole of occurring in nature silicon is wide, accounts for 27.6% in the earth's crust, but main form is can not be by the silicon-dioxide of crop utilization, and China ploughs and about 1/2 lacks the available effective silicon of plant, about 1,000,000,000 mu of area.
Silicate bacteria can be converted into the invalid silicon in the soil the utilizable effective silicon of plant.Utilize silicate bacteria to increase, and then be processed into both silicate bacteria preparations of microbial fertilizer by microorganism fermentation process.The optimum carrier of tradition silicate bacteria is the peat composed of rotten mosses, and it is cheap and can effectively guarantee the survival of silicate bacteria.
The present invention is turned waste into wealth industrial residue, and the carrier of the silicate bacteria that invention is provided can also increase the content of effective silicon in the soil except having the general character with the peat composed of rotten mosses.The Silicate Bacteria Fertilizer of utilizing this carrier to make can directly increase in the soil the effectively content of silicon, can continue again for soil provides effective silicon, remedied single silicate bacteria microbial inoculum to the slow shortcoming that gathers way of available silicon content in the soil.
[summary of the invention]
[technical problem that will solve]
The purpose of this invention is to provide a kind of silicate bacteria carrier.
Another object of the present invention provides a kind of preparation method of silicate bacteria carrier.
Another object of the present invention provides the purposes of described a kind of silicate bacteria carrier.
[technical scheme]
The present invention is achieved through the following technical solutions.
The present invention relates to a kind of silicate bacteria carrier.
Described silicate bacteria carrier is made up of following component by weight:
Peat composed of rotten mosses 50-90 part
Silicon-containing material 10-50 part;
Or
Peat composed of rotten mosses 20-70 part
Silicon-containing material 10-50 part
Wilkinite 30-50 part,
Described silicon-containing material is selected from monocalcium silicate, Dicalcium Phosphate (Feed Grade), calcium magnesium silicate, Starso, iron and steel slag, flyash shrend slag, big slag ash, yellow phosphorus furnace slag or calcium metasilicate mineral.
A preferred embodiment of the invention, described silicate bacteria carrier is made up of following component by weight:
Peat composed of rotten mosses 55-85 part
Silicon-containing material 15-45 part;
Or
Peat composed of rotten mosses 25-65 part
Silicon-containing material 15-45 part
Wilkinite 35-45 part.
A preferred embodiment of the invention, described silicate bacteria carrier is made up of following component by weight:
Peat composed of rotten mosses 60-80 part
Silicon-containing material 20-40 part;
Or
Peat composed of rotten mosses 30-60 part
Silicon-containing material 20-40 part
Wilkinite 38-42 part.
According to another kind of preferred implementation of the present invention, described silicate bacteria is selected from bacillusmusilaginosiengineering, Bacillus circulans, soil genus bacillus or adjacent pseudomonas bacillus.
According to another kind of preferred implementation of the present invention, described silicon-containing material, the peat composed of rotten mosses and bentonitic granularity are the 60-120 orders.
The invention still further relates to the preparation method of described silicate bacteria carrier, it is characterized in that this method comprises the steps:
Behind the described peat composed of rotten mosses and silicon-containing material or the described peat composed of rotten mosses, silicon-containing material and the wilkinite mixing, to 6.5-7.5, solid-to-liquid ratio is 1: 2 during mensuration, carries out granulation again with its pH regulator for usefulness acid or humic acid substance; Perhaps
Allow described silicon-containing material and described acid or humic acid substance mixing earlier and make its pH reach 6.5-7.5, mix with the peat composed of rotten mosses then or mix, carry out granulation again with the peat composed of rotten mosses and wilkinite; Perhaps
Earlier the peat composed of rotten mosses is carried out nitrated, and then mixes, or mix with silicon-containing material and wilkinite with silicon-containing material with sour, use described acid or humic acid substance with its pH regulator to 6.5-7.5, solid-to-liquid ratio is 1: 2 during mensuration, carries out granulation again.
According to a kind of preferred implementation of the inventive method, described acid is sulfuric acid, nitric acid or phosphoric acid.
According to the another kind of preferred implementation of the inventive method, the concentration of described acid is 0.1-2.0M.
According to the another kind of preferred implementation of the inventive method, described humic acid substance is ulmic acid, Ulmic acids, xanthohumic acid or nitro humus acid.
The invention still further relates to the purposes of described silicate bacteria carrier in producing fertilizer.
Below the present invention will be described in more detail.
Described silicate bacteria carrier is made up of following component by weight:
Peat composed of rotten mosses 50-90 part
Silicon-containing material 10-50 part;
Or
Peat composed of rotten mosses 20-70 part
Silicon-containing material 10-50 part
Wilkinite 30-50 part;
The described peat composed of rotten mosses is the product in the growth course of marsh, is formed at the quaternary period, and the residual body of marsh plant fails to decompose fully to be piled under the condition of many water suspicion gas.It contains multiple elements such as large quantity of moisture and the plant residue that is not thoroughly decomposed, soil ulmin and abundant nitrogen, potassium, phosphorus, calcium, manganese, is the organic substance of pure natural, is a kind of green matter of aseptic, nontoxic, nuisanceless, pollution-free, noresidue.Organic content is generally more than 30%, and proportion 0.7-1.05 is brown or black more, has flammable and air-breathing property, and the pH value is generally 5.5～6.5, is the subacidity reaction.The peat composed of rotten mosses that the present invention uses is the peat composed of rotten mosses of selling in the market, and for example Beijing benefit field source local products building materials are sold company limited, the Qing Yuanxian of Liaoning Province the earth peat development company limited, rich field, the Heilongjiang Province fertilizer company limited production peat composed of rotten mosses.
Described silicon-containing material is selected from monocalcium silicate, Dicalcium Phosphate (Feed Grade), calcium magnesium silicate, Starso, iron and steel slag, flyash shrend slag, big slag ash, yellow phosphorus furnace slag or calcium metasilicate mineral.
Described monocalcium silicate, Dicalcium Phosphate (Feed Grade), calcium magnesium silicate, Starso all are product solds in the market.
The iron and steel slag is the slag that produces in iron smelting steel process, and the iron and steel slag mainly is made up of the oxide compound of calcium, iron, silicon, magnesium and small amount of aluminum, manganese, phosphorus etc.Main mineral facies are the Solid solution that the oxide compound of tricalcium silicate, Dicalcium Phosphate (Feed Grade), monticellite, calcium manganolite, iron calcium aluminate and silicon, magnesium, iron, manganese, phosphorus forms, and also contain small-amount free calcium oxide and metallic iron, fluorapatite etc.The iron and steel slag that the present invention uses is the iron and steel slag that Ma'anshan Iron and Steel Co., Ltd, Tangshan Iron ﹠ Steel Group Co., Ltd produce.
Flyash shrend slag is the pure slag of water of heat power plant's vertical cyclone furnace discharging, and the pure slag of water of coal ash about 150,000 tons can discharge in 1 year, wherein in a national large-lot producer; The flyash shrend slag that the present invention uses for example is the flyash shrend slag that the thermoelectric limited liability company in Harbin, Qinhuangdao heat power plant produce.
Big slag ash is the pure slag of water of heat power plant's vertical cyclone furnace discharging, is the irregular particle shape, and pH neutrality contains effective SiO
2Average more than 20%; The big slag ash that the present invention uses for example is the big slag ash that the thermoelectric limited liability company in Harbin, Qinhuangdao heat power plant produce.
Yellow phosphorus furnace slag is the industrial residue in chemical plant, contains effective SiO
2About 20%, pH neutrality; The yellow phosphorus furnace slag that the present invention uses for example is the yellow phosphorus furnace slag that phosphorus city, Kaiyang, Guizhou Province phosphorous chemical industry company limited, Guizhou Wengfu Yellow Phosphorus Co., Ltd. produce.
Calcium metasilicate mineral (wollastonite) are a kind of non-metallic mineralss of natural output, and chemical molecular formula is CaSiO
3, being needle-like, fibrous agrregate usually, color is pure white.The calcium metasilicate mineral that the present invention uses for example are the calcium metasilicate mineral that chemical industry company limited far away produces in the bright Feng Fenticailiaochang in Lishu County, the permanent industry in Beijing.
Described wilkinite is to be the nonmetallic minerals of essential mineral composition with the montmorillonite, 2: 1 type crystal structure that smectite structure is made up of two silicon-oxy tetrahedron folder layer of aluminum oxygen octahedras, because there is some positively charged ion in the laminate structure that the montmorillonite structure cell forms, as Cu, Mg, Na, K etc., and the effect of these positively charged ions and montmorillonite structure cell is very insecure, easily by other cationic exchange, so have ion exchangeable preferably.The wilkinite that the present invention uses can be various types of wilkinites, sodium bentonite for example, the sodium bentonite of producing as good prosperous wilkinite factory of Shandong Xian Quan wilkinite factory, Xinyang; Calcium-base bentonite, the calcium-base bentonite of producing as company of good prosperous wilkinite factory of Shandong Xian Quan wilkinite factory, Xinyang; The hydrogen base bentonite grinds the hydrogen base bentonite of former new science and technology limited Company, the production of concentric wilkinite factory as Beijing; Organobentonite, the organobentonite of producing as the good prosperous wilkinite in Xinyang factory, sky, Anji, Zhejiang dragon organobentonite company limited.
The described peat composed of rotten mosses, silicon-containing material and bentonitic granularity are controlled at the 60-120 order, preferably the 80-100 order.If the described peat composed of rotten mosses, silicon-containing material and bentonitic granularity are greater than 120 orders, loft is low, and ventilation property is bad, easily caking; If the described peat composed of rotten mosses, silicon-containing material and bentonitic granularity are less than 60 orders, then ventilation property is strong, water conservation not, and microbial inoculum is easily air-dry.
Therefore, if the described peat composed of rotten mosses, silicon-containing material and wilkinite need to sieve before use, get part use of 60-120 purpose sieve.If the described peat composed of rotten mosses, silicon-containing material and bentonitic granularity greater than 120 orders, then need to pulverize, screening then is to obtain 60-120 purpose raw material.Described pulverizing all is to adopt normally used disintegrating apparatus and screening plant in the present technique field with screening, and these disintegrating apparatus and screening plant also are general in the market product solds.
Preferably, described silicate bacteria carrier is made up of following component by weight:
Peat composed of rotten mosses 55-85 part
Silicon-containing material 15-45 part;
Or
Peat composed of rotten mosses 25-65 part
Silicon-containing material 15-45 part
Wilkinite 35-45 part.
More preferably, described silicate bacteria carrier is made up of following component by weight:
Peat composed of rotten mosses 60-80 part
Silicon-containing material 20-40 part.
Or
Peat composed of rotten mosses 30-60 part
Silicon-containing material 20-40 part
Wilkinite 38-42 part.
The present invention relates to the silicate bacteria carrier, described silicate bacteria can be decomposed the ecosystem mineral of aluminum silicates such as feldspar, mica, soil mineral on the one hand, make in the soil insoluble K, P, Si etc. change soluble substance into for the plant-growth utilization, the potassium that is rich in the thalline is absorbed by crop again after bacterium death; Materials such as the hormone that it produced, amino acid, polysaccharide promote the growth of crop on the other hand.Simultaneously, bacterium breeds in soil, suppresses other growth of pathogenic bacteria.These all plant growth, output are improved and quality improving has good action.
According to the present invention, described silicate bacteria is selected from bacillusmusilaginosiengineering, Bacillus circulans, soil genus bacillus or adjacent pseudomonas bacillus.Disclosed in the prior art cultural method is adopted in the cultivation of described silicate bacteria.
The invention still further relates to the preparation method of described silicate bacteria carrier.
This method comprises the steps:
Behind the described peat composed of rotten mosses and silicon-containing material or the described peat composed of rotten mosses, silicon-containing material and the wilkinite mixing, usefulness acid or humic acid substance to 6.5-7.5, carry out granulation with its pH regulator again.
The described peat composed of rotten mosses, silicon-containing material and wilkinite need to carry out conventional pre-treatment before application.For example their water-content is too high, for example reaches more than 20% (weight), then needs to carry out drying treatment, and their water-content is controlled at below 10% (weight).When their granularity is excessive or too small, need carries out pulverization process and handle, make their granularity reach the 60-120 order with screening.
Use mixing equipment with the pretreated peat composed of rotten mosses and silicon-containing material mixing then, perhaps with the pretreated peat composed of rotten mosses, silicon-containing material and wilkinite mixing.Described mixing equipment can be the mechanical means that the abundant mixing of these raw materials can not be taken place in other side reaction, for example normally used stirrer, duplex cone-type mixer etc. in the present technique field.
Use again behind the described material mixing acid or humic acid substance with its pH regulator to 6.5-7.5.
Can use many organic acids or mineral acid to carry out this adjusting, but consider production cost and, preferably use sulfuric acid, nitric acid or phosphoric acid, use sulfuric acid, nitric acid or phosphate aqueous solution to regulate usually later on to the influence of soil and crop.The concentration of described acid is 0.1-2.0M, preferably 0.5-1.0M.
Using humic acid substance to carry out this adjusting is to realize by the acidic-group in them.In the present invention, described humic acid substance for example is ulmic acid, Ulmic acids, xanthohumic acid or nitro humus acid.Humic acid substance is the natural macromole aromatic hydroxy-carboxylic of a class.It extensively is present in the soil organism, peat, brown coal, weathered coal and lake and the oceanic sediment.Coal can form the regeneration humic acids through artificial oxidation's (as with air, ozone or nitric acid treatment), is called nitro humic acid as coal with the product of nitric acid mild oxidation gained.The extracting method of humic acids is to use acid treatment earlier, sloughs the part mineral substance, and with the dilute alkaline soln extraction, extraction liquid is acidified with acid again, obtains the humic acids precipitation.Can be divided into three components according to the solubleness of humic acids in solvent: the part that 1. is dissolved in acetone or alcohol is called ulmic acid; 2. be insoluble to partial acetone and be called Ulmic acids; Part 3. water-soluble or diluted acid is called xanthohumic acid (claiming fulvic acid again).The macromolecular basic structure of humic acids is aromatic ring and alicyclic ring, and functional groups such as carboxyl, hydroxyl, carbonyl, quinonyl, methoxyl group are arranged on the ring.They and metal ion have effects such as exchange, absorption, complexing, chelating.Described humic-acid kind thing consumption requires to determine according to regulating pH.
The adjusting of pH value has met the requirements of raw material and need carry out granulation.Agglomeration techniques such as described granulation can the selector disc granulation, extruder grain, drum granulating, these technology all are the technology that those skilled in the art know, the equipment that these agglomeration techniques use is the normally used equipment of people in the present fertilizer production technology field, also is extensive in the market product sold.
Product needed after the granulation is carried out drying and is handled with screening, these dryings all are the technology that those skilled in the art know with material sieving technology, these dry equipment that use with material sieving technology are the normally used equipment of people in the present fertilizer production technology field, also are extensive in the market product solds.
Perhaps, the preparation method of silicate bacteria carrier of the present invention comprises the steps:
Allow described silicon-containing material and described acid or humic acid substance mixing earlier and make its pH reach 6.5-7.5, mix with the peat composed of rotten mosses then or mix, carry out granulation again with the peat composed of rotten mosses and wilkinite.
Perhaps, the preparation method of silicate bacteria carrier of the present invention comprises the steps:
Earlier the peat composed of rotten mosses is carried out nitrated, and then mixes, or mix with silicon-containing material and wilkinite with silicon-containing material with sour, use described acid or humic acid substance with its pH regulator to 6.5-7.5, carry out granulation again.
Other treatment step does not repeat them here as previously described.
The silicate bacteria carrier of the present invention's preparation and bacillusmusilaginosiengineering fermented liquid, Bacillus circulans fermented liquid etc. carry out mixing and absorption, fully promptly can use according to conventional amount used and usage as microbial fertilizer behind the mixing.
Adopt the silicate bacteria carrier of the present invention's preparation can guarantee that silicate bacteria survives for a long time, it can be played one's part to the full, to effective silicon that can directly be utilized by crop can not be become in the soil, thereby the effectively content of silicon can be significantly improved in the soil by the silicon that crop directly utilizes.
[beneficial effect]
Beneficial effect of the present invention is as follows:
Compare as the carrier of silicate bacteria with traditional single peat composed of rotten mosses, use the silicate bacteria carrier of the present invention preparation just can make Silicate Bacteria Fertilizer continue to provide effective silicon, overcome the single silicate bacteria microbial inoculum available silicon content slow defective that gathers way fully as crop from beginning.
[embodiment]
Embodiment 1
Iron and steel slag and Qian'an that present embodiment uses Tangshan Iron ﹠ Steel Group Co., Ltd to produce are unified into the peat composed of rotten mosses that wilkinite company limited produces, and use the 80-100 mesh sieve to sieve, and obtain 80-100 order iron and steel slag, the peat composed of rotten mosses respectively.
The described iron and steel slag of 0.8kg and the described peat composed of rotten mosses of 1.6kg are still mixed in the groove of the band stirring rake produced of your fluid device company limited in Shanghai, under agitation slowly add 33% (weight) H then
25O
4The aqueous solution, the pH that the DELTAPH instrumentation that uses Shanghai precision instrument company limited to produce is simultaneously decided its mixture (solid-to-liquid ratio is 1: 2) is 7.0, the H of use
2SO
4The aqueous solution is 120ml, then stirs up to abundant mixing again, and the mixture that obtains is as the powder carrier of silicate bacteria.
Above-mentioned carrier and bacillusmusilaginosiengineering fermented liquid are proportionally adsorbed by weight at 5: 2, fully adopting the dilution plate method of counting under normal temperature condition, to carry out regular live bacterial count mensuration behind the mixing, adopt the silicon molybdenum blue colorimetric method under normal temperature condition, to measure the available silicon content (referring to " the soil agrochemistry analysis third edition ") of the silicate bacteria carrier of present embodiment preparation.
Meanwhile carried out controlled trial, controlled trial is used the pH6.5 peat composed of rotten mosses (pH regulates with light calcium carbonate).Carried out live bacterial count every 1 month, and measure the content of its effective silicon, be 6 months detection time.Detected result is listed in table 1.
Table 1: the measurement result of viable count and available silicon content
Embodiment 2
Iron and steel slag, the Qian'an that present embodiment uses Tangshan Iron ﹠ Steel Group Co., Ltd to produce is unified into the peat composed of rotten mosses of wilkinite company limited production, the wilkinite that Shandong Xian Quan wilkinite factory produces, use the 80-100 mesh sieve to sieve, obtain 80-100 order iron and steel slag, the peat composed of rotten mosses, wilkinite respectively.
The 0.9kg peat composed of rotten mosses and 1.5kg80 order iron and steel slag, 1.8kg wilkinite after Shanghai still mixes in the groove of the band stirring rake produced of your fluid device company limited, are under agitation slowly added 25% (weight) HNO then
3The aqueous solution, the DELTAPH instrumentation that uses Shanghai precision instrument company limited to produce is simultaneously decided the pH6.97 of its mixture (solid-to-liquid ratio is 1: 2), the HNO of use
3The aqueous solution is 270ml.Then stir again up to abundant mixing, the mixture that obtains carries out extruder grain in the fertilizer tablets press that machine works of Weifang City's agricultural machine institute produces after as the granular carrier of silicate bacteria.
Above-mentioned carrier and Bacillus circulans fermented liquid were adsorbed by 4: 1, fully adopting the dilution plate method of counting under normal temperature condition, to carry out regular live bacterial count mensuration behind the mixing, adopt the silicon molybdenum blue colorimetric method under normal temperature condition, to measure the available silicon content of the silicate bacteria carrier of present embodiment preparation.
Meanwhile carried out controlled trial, controlled trial is used the pH6.5 peat composed of rotten mosses (pH regulates with light calcium carbonate).Carried out live bacterial count every 1 month, and measure the content of its effective silicon, be 6 months detection time.Detected result is listed in table 2.
Table 2: the measurement result of viable count and available silicon content
Embodiment 3
Big slag ash, the Qian'an that present embodiment uses Qinhuangdao heat power plant to produce is unified into the peat composed of rotten mosses of wilkinite company limited production, the wilkinite that Shandong Xian Quan wilkinite factory produces uses the 80-100 mesh sieve to sieve, and obtains 80-100 order iron and steel slag, the peat composed of rotten mosses, wilkinite respectively.
With the big slag ash of 2kg80 purpose, the 3kg peat composed of rotten mosses, 5kg wilkinite after Shanghai still mixes in the groove of the band stirring rake produced of your fluid device company limited; use the pH6.8 of fixed its mixture (solid-to-liquid ratio is 1: 2) of DELTA PH instrumentation that Shanghai precision instrument company limited produces, mixture carries out extruder grain in the fertilizer tablets press that machine works of Weifang City's agricultural machine institute produces after as the granular carrier of silicate bacteria.
Above-mentioned carrier and soil fermentation of bacillus liquid were adsorbed by 4: 1, fully adopting the dilution plate method of counting under normal temperature condition, to carry out regular live bacterial count mensuration behind the mixing, adopt the silicon molybdenum blue colorimetric method under normal temperature condition, to measure the available silicon content of the silicate bacteria carrier of present embodiment preparation.
Meanwhile carried out controlled trial, controlled trial is used the pH6.5 peat composed of rotten mosses (pH regulates with light calcium carbonate).Carried out live bacterial count every 1 month, and measure the content of its effective silicon, be 6 months detection time.Detected result is listed in table 3.
Table 3: the measurement result of viable count and available silicon content
Embodiment 4
Flyash shrend slag and Qian'an that present embodiment uses Qinhuangdao heat power plant to produce are unified into the peat composed of rotten mosses that wilkinite company limited produces, and use the 80-100 mesh sieve to sieve, and obtain 80-100 order iron and steel slag, the peat composed of rotten mosses respectively.
2kg80 purpose flyash shrend slag and the 1kg peat composed of rotten mosses, 30g nitro humic acid are still mixed in the groove of the band stirring rake produced of your fluid device company limited in Shanghai, the pH that the DELTA PH instrumentation that uses Shanghai precision instrument company limited to produce is decided its mixture (solid-to-liquid ratio is 1: 2) is 6.7, and the mixture that obtains is as the powder carrier of silicate bacteria.
Above-mentioned carrier and colloid bacillus cereus fermented liquid are proportionally adsorbed by weight at 5: 2, fully adopting the dilution plate method of counting under normal temperature condition, to carry out regular live bacterial count mensuration behind the mixing, adopt the silicon molybdenum blue colorimetric method under normal temperature condition, to measure the available silicon content of the silicate bacteria carrier of present embodiment preparation.
Meanwhile carried out controlled trial, controlled trial is used the pH6.5 peat composed of rotten mosses (pH regulates with light calcium carbonate).Carried out live bacterial count every 1 month, and measure the content of its effective silicon, be 6 months detection time.Detected result is listed in table 4.
Table 4: the measurement result of viable count and available silicon content
Embodiment 5: the soil culture experiment of silicate bacteria pulvis carrier
1) gets Changli, Qinhuangdao rice soil and dry in the shade, cross the 2mm sieve;
2) measure the saturation moisture content a of this soil;
3) get the back rice soil 500g that sieves, in the 500ml plastic beaker of packing into behind the complete mixing of silicate bacteria carrier b 1g, add tap water 150ml to the soil water content state that reaches capacity, the beaker mouth seals the back with plastic film and pricks a small amount of hole, be put at last in 25 ℃ of incubators, measure available silicon content c in the soil in the 1st, 2,4,6,8 weeks respectively.The controlled trial that does not add the silicate bacteria carrier simultaneously.Detected result is listed in table 5.
Table 5: silicate bacteria carrier soil culture experiment result
| Effective silicon mg/kg | 1 week | 2 weeks | 4 weeks | 6 weeks | 8 weeks |
| ck | 218.4 | 218.6 | 218.9 | 219.5 | 220.1 |
| With microbial inoculum carrier b | 357.3 | 357.9 | 358.4 | 358.9 | 359.4 |
Saturated soil water content a measuring method is as follows:
A) get a certain amount of test and put into Bush's funnel with soil, constantly slowly add water, the soil water content state that reaches capacity leaves standstill, spends the night when treating that water does not almost have water droplet to fall in the funnel.This test gained saturation moisture content is 23.1%.
B) get quantitative above-mentioned soil m
1G puts into the aluminium box m that has weighed
2Among the g, add a small amount of alcohol, light, soil is dried out fully after, m weighs
3G.
C) calculating of saturated water content in the soil:
B: silicate bacteria carrier: the described iron and steel slag of 0.8kg and the described peat composed of rotten mosses of 1.6kg are still mixed in the groove of the band stirring rake produced of your fluid device company limited in Shanghai, under agitation slowly add 33% (weight) H then
2SO
4The aqueous solution, the pH that the DELTA PH instrumentation that uses Shanghai precision instrument company limited to produce is simultaneously decided its mixture (solid-to-liquid ratio is 1: 2) is 7.0, the H of use
2SO
4The aqueous solution is 120ml, then stirs up to abundant mixing again, and the mixture that obtains is as the powder carrier of silicate bacteria, available silicon content 9.2%.Effectively the measuring method of silicon is that silica test is according to molybdenum blue colorimetric method (referring to " the soil agrochemistry analysis third edition ").
Claims (10)
1. silicate bacteria carrier is characterized in that it is made up of following component by weight:
Peat composed of rotten mosses 50-90 part
Silicon-containing material 10-50 part;
Or
Peat composed of rotten mosses 20-70 part
Silicon-containing material 10-50 part
Wilkinite 30-50 part.
Described silicon-containing material is selected from monocalcium silicate, Dicalcium Phosphate (Feed Grade), calcium magnesium silicate, Starso, iron and steel slag, flyash shrend slag, big slag ash, yellow phosphorus furnace slag or calcium metasilicate mineral.
2. silicate bacteria carrier according to claim 1 is characterized in that it is made up of following component by weight:
Peat composed of rotten mosses 55-85 part
Silicon-containing material 15-45 part;
Or
Peat composed of rotten mosses 25-65 part
Silicon-containing material 15-45 part
Wilkinite 35-45 part.
3. silicate bacteria carrier according to claim 1 is characterized in that it is made up of following component by weight:
Peat composed of rotten mosses 60-80 part
Silicon-containing material 20-40 part;
Or
Peat composed of rotten mosses 30-60 part
Silicon-containing material 20-40 part
Wilkinite 38-42 part.
4. silicate bacteria carrier according to claim 1 is characterized in that described silicate bacteria is selected from bacillusmusilaginosiengineering, Bacillus circulans, soil genus bacillus or adjacent pseudomonas bacillus.
5. silicate bacteria carrier according to claim 1 is characterized in that described silicon-containing material, the peat composed of rotten mosses and bentonitic granularity are the 60-120 orders.
6. according to the preparation method of the described silicate bacteria carrier of each claim among the claim 1-5, it is characterized in that this method comprises the steps:
Behind the described peat composed of rotten mosses and silicon-containing material or the described peat composed of rotten mosses, silicon-containing material and the wilkinite mixing, to 6.5-7.5, solid-to-liquid ratio is 1: 2 during mensuration, carries out granulation again with its pH regulator for usefulness acid or humic acid substance; Perhaps
Allow described silicon-containing material and described acid or humic acid substance mixing earlier and make its pH reach 6.5-7.5, mix with the peat composed of rotten mosses then or mix, carry out granulation again with the peat composed of rotten mosses and wilkinite; Perhaps
Earlier the peat composed of rotten mosses is carried out nitrated, and then mixes, or mix with silicon-containing material and wilkinite with silicon-containing material with sour, use described acid or humic acid substance with its pH regulator to 6.5-7.5, solid-to-liquid ratio is 1: 2 during mensuration, carries out granulation again.
7. method according to claim 6 is characterized in that described acid is sulfuric acid, nitric acid or phosphoric acid.
8. method according to claim 6, the concentration that it is characterized in that described acid is 0.1-2.0M.
9. method according to claim 6 is characterized in that described humic acid substance is ulmic acid, Ulmic acids, xanthohumic acid or nitro humus acid.
10. according to claim 1 or according to the purposes of silicate bacteria carrier in producing fertilizer of the described method of claim 6 preparation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105801241A (en) * | 2016-02-22 | 2016-07-27 | 鹤壁市人元生物技术发展有限公司 | Silicate bacteria fertilizer and preparation method thereof |
| CN107986908A (en) * | 2017-12-06 | 2018-05-04 | 云南云天化股份有限公司 | A kind of microbial manure matrix of suitable bacillus growth |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1119178A (en) * | 1995-08-31 | 1996-03-27 | 金尧 | Yeast microbial fertilizer and producing process thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1119178A (en) * | 1995-08-31 | 1996-03-27 | 金尧 | Yeast microbial fertilizer and producing process thereof |
Non-Patent Citations (2)
| Title |
|---|
| 《土壤肥料》 19971231 吴观以等 用海绿石作为载体吸附硅酸盐细菌的初步研究 第43-45页 1-10 , 第2期 * |
| 《土壤肥料》 19991231 李永兴等 不同载体对微生物菌剂质量的影响 第31-32页 1-10 , 第6期 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105801241A (en) * | 2016-02-22 | 2016-07-27 | 鹤壁市人元生物技术发展有限公司 | Silicate bacteria fertilizer and preparation method thereof |
| CN107986908A (en) * | 2017-12-06 | 2018-05-04 | 云南云天化股份有限公司 | A kind of microbial manure matrix of suitable bacillus growth |
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