CN102172476B - Dialysis membrane fixing device - Google Patents
Dialysis membrane fixing device Download PDFInfo
- Publication number
- CN102172476B CN102172476B CN 201110072365 CN201110072365A CN102172476B CN 102172476 B CN102172476 B CN 102172476B CN 201110072365 CN201110072365 CN 201110072365 CN 201110072365 A CN201110072365 A CN 201110072365A CN 102172476 B CN102172476 B CN 102172476B
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- holder
- dialysis membrane
- dialysis
- quill
- chuck
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- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention provides a dialysis membrane fixing device comprising a permanent seat and a jacket, wherein the permanent seat is a hollow cylinder; the jacket is clamped on the outer side wall of the permanent seat; the outer side wall of the permanent seat and the jacket are provided with holes of which the positions correspond; when the fixing device is used, a dialysis membrane cut into a stripy shape is unfolded to be clamped between the outer side wall of the permanent seat and the jacket; the upper end of the permanent seat is connected with a movable sleeve by virtue of a fastening ring and a seal ring; the inner bottom and outer bottom of the permanent seat are respectively provided with a magnetic stir bar; the lower end of the permanent seat is provided with supporting legs; and the upper end of the movable sleeve is provided with a sample feeding hole and a sample feeding hole cover. The dialysis membrane fixing device can be used for greatly improving the dialysis efficiency, and is favorable for samples to remain in an aseptic condition in the dialysis and transfer process. By utilizing the dialysis membrane fixing device, the dialysis under the positive pressure and negative pressure condition can be carried out, and the dialysis membrane cut into the stripy shape is easy to clean, so that the use times are increased, and the cost is lowered.
Description
Technical field
The present invention relates to the apparatus of dialysing, especially be used for gripping dialysis membrane so that the device of dialysing.
Background technology
Dialysis is one of laboratory common technology, is used for macromolecular substances such as protein displacement buffer system, thick purifying, concentrates, desalination etc.The core component of dialysis technology is a dialysis membrane, and the dialysis membrane that spreads all over micropore allows to arrive the dialysis membrane opposite side less than the molecular diffusion of micro-pore diameter, and therefore dialysis is able to carry out.The dialysis membrane that is generally materials such as cellulose, cellulose mixture of laboratory use at present, dialysis membrane is made into bag filter, dialysis tubing, during dialysis sample liquid is packed into wherein, and airtight being placed in the dislysate dialysed.Bag filter, dialysis tubing are very easy to use, need not specific apparatus, therefore use extensively.But the motion mode of sample molecule is freely to spread in bag filter, the dialysis tubing, and after a period of time was carried out in dialysis, approaching zero near the concentration difference of dialysis membrane part target molecule, dialysis almost stopped, and almost not become away from dialysis membrane part concentration of target molecules this moment; Later dialysis just recovers once more near the concentration difference of dialysis membrane part when target molecule has improved through diffusion, so dialytic efficiency is not given full play to.When bag filter, dialysis tubing diameter are big, the influence of the uneven effect of the concentration difference highly significant that becomes, dialytic efficiency significantly reduces, and it is very long to reach the required time of complete equipilibrium; The diffusion rate of macromolecule target molecule is little in addition, even the dialysis of sample also needs just can reach complete equipilibrium for a long time in a small amount.The concentration difference inequality is the greatest drawback of bag filter, dialysis tubing in the dialysis procedure; Be through making sample in the closed environment that dialysis membrane, pipeline, pump are formed, keep flow regime to solve this problem in the clinical practice, but the laboratory does not also have corresponding product at present.
The other defective of bag filter, dialysis tubing comprises: sink to the dislysate bottom after (1) bag filter seals with sealing clip usually; The bag filter quantity stirrer in the dislysate for a long time usually can not normal rotation; Though there is not this problem in dialysis tubing, use cost is high; (2) bag filter, dialysis tubing use the back to be difficult for cleaning; The mode that can only adopt flowing water to wash repeatedly cleans, and cleaning effect is relatively poor, the inboard normal residual sample of film; If each experiment all uses new bag filter, dialysis tubing then can improve experimental cost, and not environmental protection; (3) mechanical strength of bag filter, dialysis tubing is not high, some need pressurize or the environmental condition of negative pressure under can't use; (4) after bag filter, the dialysis tubing aseptic process, germ-free condition is difficult for keeping in the use, especially in the sample transfer process, pollutes easily.
Summary of the invention
The purpose of this invention is to provide a kind of dialysis experiment that is convenient to do, can improve dialytic efficiency, and help to make sample in dialysis and transfer process, to keep the dialysis membrane fixture of germ-free condition.
For realizing above-mentioned technical purpose; The invention provides a kind of dialysis membrane fixture; Comprise holder and chuck, holder is a hollow column, and the chuck clip is in the lateral wall of holder; Holder lateral wall and chuck are provided with corresponding hole, position, and dialysis membrane is unfolded and is held between holder lateral wall and the chuck during use.
The dialysis membrane that is cut into strip is held between holder lateral wall and the chuck, forms a confined space to hold sample, can place in inside or fixed installation magnetic agitation.Apparatus of the present invention that sample is housed are submerged in the dislysate; Sample molecule just carries out diffusion motion through hole on holder lateral wall and the chuck and dialysis membrane; Magnetic agitation makes externally that each several part sample concentration difference remains homogeneous in the confined space under the effect in magnetic field; Thereby the raising dialytic efficiency significantly reduces the required time of dialysis equilibrium that reaches.Dialysis membrane can be pulled down and clean after dialysis was accomplished, and improved cleaning effect thus, had increased the dialysis membrane access times, helped to reduce cost.In addition, because holder and chuck are that dialysis membrane provides support, have disperseed the suffered pressure of dialysis membrane, thereby can carry out the dialysis under some malleations or the condition of negative pressure.
Further, the upper end of holder is connected with quill through dead ring, sealing ring, and sealing ring is between holder and quill lateral wall and the dead ring.The upper end inward flange of holder and sealing ring lateral surface are the taper surface that matches.Between dead ring and the holder for being threaded; When the rotation dead ring moves down it; Dead ring is promoting sealing ring and is moving down, and the taper surface of holder upper end inward flange produces axially and the radial compression effect sealing ring, thereby the clearance seal between holder, dead ring and the quill three is lived; And compress quill, quill is reliably located.
Further; Quill can move up and down, thereby makes holder internal capacity adjustable size, and the holder inwall is provided with by the position, upper end quill is carried out spacing boss; When moving down quill, can play position-limiting action to the range that quill moves down in the process; The quill lateral wall is provided with the scale of vertical direction, just can determine the volume of confined space in the holder according to this scale; The quill upper end is provided with well and application of sample port lid, and well is lived by application of sample port lid closed cover in the dialysis procedure, makes inside be in germ-free condition, and sample shifted through well after dialysis was accomplished, so sample can be not contaminated in transfer process.
Further, chuck is provided with two of symmetry, connects for buckle-type between two chucks.Being clamped on the holder with two dialysis membrane symmetries during use.Adopt the buckle-type syndeton of two symmetrical chucks, be convenient to the manufacturing and the assembling of chuck, also make the dismounting of dialysis membrane easier.
Corresponding hole, position is the bar hole of horizontal direction on holder lateral wall and the chuck, is provided with seal groove around the bar hole, and sealing strip is housed in the seal groove.In vertical direction, the rib between two bar holes is satisfying under the prerequisite of support strength narrowly as much as possible up and down, makes the hole big as much as possible, to improve effective usable floor area of dialysis membrane.
The inner bottom part of holder is provided with first magnetic agitation, and outer bottom is provided with second magnetic agitation.During dialysis; First magnetic agitation, second magnetic agitation externally magnetic field effect stir sample liquid, dislysate respectively down; To keep the homogeneous of sample liquid, dislysate each several part concentration difference in dialysis procedure, significantly improve dialytic efficiency, reduce the time that reaches dialysis equilibrium.
The lower end of holder is provided with at least two feets, and the lower end of feet radially outward is extended with flange, and flange upwards is a wedge that big other end is little in week.Utilize feet, can holder be positioned in the container of splendid attire dislysate, the feet lower end is provided with the wedge flange, is in order to adopt rotation mode fast holder to be positioned.
The remarkable technique effect that the present invention obtains is: dialysis membrane is cut into strip is clamped on the device of the present invention and dialyses; Can not only improve dialytic efficiency greatly; Conveniently dialysis membrane being cleaned the back reuses; Reduce the dialysis experimental cost, and can carry out the dialysis under some malleations or the condition of negative pressure, and help to make sample in dialysis and transfer process, to keep germ-free condition.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Fig. 2 is a perspective exploded view of the present invention.
Fig. 3 is the floor map after Fig. 1 dissects along the A-A line.
Fig. 4 is the B-B profile of Fig. 3.
Among the figure: 1. holder; 2. chuck; 3. hole; 4. dead ring; 5. quill; 6. scale; 7. well; 8. application of sample port lid; 9. first sealing ring; 10. first magnetic agitation is sub; 11. second magnetic agitation is sub; 12. second sealing ring; 13. anti-skidding convexity; 14. boss; 15. sealing strip; 16. feet; 17. flange; 18. salient point; 19. groove.
The specific embodiment
Below in conjunction with the accompanying drawing and the specific embodiment the present invention is specified.
To shown in Figure 4, a kind of dialysis membrane fixture is used for the dialysis membrane that cutting is in blocks to grasp the experiment of dialysing like Fig. 1.This device includes holder 1, chuck 2 and quill 5, and dialysis membrane is unfolded and is held between holder 1 lateral wall and the chuck 2 during use, and holder 1 lateral wall and chuck 2 are provided with corresponding hole 3, position.Holder 1, dialysis membrane, quill 5 be common to constitute confined spaces, interiorly is equivalent to bag filter or dialysis tubing in order to hold sample liquid, and sample molecule spreads through hole 3 and the dialysis membrane on holder 1 lateral wall and the chuck 2.
Referring to Fig. 2, Fig. 3 and Fig. 4; The upper end of holder 1 is connected with quill 5 through dead ring 4, first sealing ring 9; Quill 5 lateral walls are provided with the scale 6 of vertical direction, and quill 5 is complementary the lower ending opening of quill 5 with the profile of holder 1; The upper end is provided with well 7 and application of sample port lid 8, between dead ring 4 and the holder 1 for being threaded.Quill 5 end faces can be set to sphere, and well 7 evaginations are arranged at the central authorities of end face, and application of sample port lid 8 is equiped with second sealing ring 12 through being threadedly connected on the well 7 at application of sample port lid 8 inner tops.Scale 6 both can application formation also can adopt the scale form to indicate.Unclamp dead ring 4, can move up and down quill 5, thereby make apparatus of the present invention sealed volume adjustable size, just can confirm the volume size of confined space, be convenient to the sample liquid that adds is carried out precisely quantitatively according to the scale on quill 5 lateral walls 6.
Like Fig. 1, Fig. 2 and shown in Figure 3, the inner bottom part of holder 1 is provided with first magnetic agitation 10, and outer bottom is provided with second magnetic agitation 11.First magnetic agitation, 10, second magnetic agitation 11 so that the concentration of sample liquid, dislysate each several part keeps homogeneous, thereby improves dialytic efficiency in order to when dialysing, respectively sample liquid, dislysate are stirred greatly.
Referring to Fig. 3, Fig. 4, the upper end inward flange of holder 1 is a taper surface, and the lateral surface that is provided with first sealing ring, 9, the first sealing rings 9 between this taper surface and quill 5 lateral walls and the dead ring 4 also is a taper surface.When rotation dead ring 4 moves down it; Dead ring 4 is promoting first sealing ring 9 and is moving down; The taper surface of holder 1 upper end inward flange produces axially and the radial compression effect first sealing ring 9; Thereby the clearance seal between holder 1, dead ring 4 and quill 5 threes is lived, and compress quill 5, quill 5 is reliably located.
Referring to Fig. 1, Fig. 2 and Fig. 3, the lateral surface of dead ring 4 is anti-skidding protruding 13 along being circumferentially with, and when twisting dead ring with hand-screw, can avoid skidding.
Like Fig. 3, shown in Figure 4; Holder 1 inwall is provided with by the position, upper end quill 5 is carried out spacing boss 14; When moving down quill 5, can play position-limiting action to the range that quill 5 moves down in the process, prevent that quill 5 from falling in the holder 1.
Corresponding hole 3, position is the bar hole of horizontal direction on holder 1 lateral wall and the chuck 2, is provided with seal groove around the bar hole, and sealing strip 15 is housed in the seal groove.Holder 1 whole lateral surface porose 3 area sum promptly be the gross area that dialysis membrane can be exposed; Therefore in vertical direction; Rib between two bar holes should be done narrowlyer as far as possible up and down; Make hole 3 big as much as possible under the prerequisite of bulk strength satisfying, to improve effective usable floor area of dialysis membrane.Vertical bar hole also can be made in hole 3, or makes other shape.It is preferably whole more recessed that holder 1 lateral wall is used for the zone of clamping dialysis membrane, so that the properer clamping of dialysis membrane above that.Sealing strip position on holder 1 lateral wall sealing strip and the chuck 2 preferably misplaces a bit each other, to improve sealing reliability.
Referring to Fig. 1, Fig. 2, holder 1 is a hollow cylinder shape, and chuck 2 is provided with two of symmetry, connects through buckle between two chucks 2, and cylindrical shape helps its interior sample molecule and carries out balanced diffusion to all directions.During use, with being clamped on the holder 1 of two dialysis membrane symmetries.Adopt the buckle-type syndeton of two symmetrical chucks, be convenient to the manufacturing and the assembling of chuck 2, also make the dismounting of dialysis membrane easier.Salient point 18 is arranged on the chuck 2, and correspondence position is fluted 19 on the holder 1, and salient point 18 cooperates with groove 19 can play the auxiliary fixing effect to chuck.
The lower end of holder 1 is provided with at least two feets 16, and the lower end of feet 16 radially outward is extended with flange 17, and flange 17 upwards is a wedge that big other end is little in week.Utilize feet 16, can holder 1 be positioned in the container of splendid attire dislysate, feet 16 lower ends are provided with wedge flange 17, are in order to adopt rotation mode fast holder 1 to be positioned.
The actual use shows that interior external application magnetic agitation stirs sample and dislysate respectively during dialysis, has improved dialytic efficiency greatly; Sample shifts through well in the dialysis procedure, is easy to keep germ-free condition; Because holder and chuck are that dialysis membrane provides support, have disperseed the suffered pressure of dialysis membrane, thereby can carry out the dialysis under some malleations or the condition of negative pressure simultaneously; And the dialysis membrane that dialysis is cut into strip after accomplishing can clean completely, increased access times, helps to reduce the dialysis experimental cost.
Claims (9)
1. dialysis membrane fixture, it is characterized in that: comprise holder and chuck, holder is a hollow column, and the chuck clip is in the lateral wall of holder, and holder lateral wall and chuck are provided with corresponding hole, position; Dialysis membrane is unfolded and is held between holder lateral wall and the chuck during use; The inner bottom part of holder is equipped with first magnetic agitation.
2. a kind of dialysis membrane fixture as claimed in claim 1, it is characterized in that: the upper end of said holder is connected with quill through dead ring and sealing ring, between dead ring and the holder for being threaded.
3. a kind of dialysis membrane fixture as claimed in claim 1, it is characterized in that: the profile of said chuck and holder is complementary, and is provided with two of symmetry, connects for buckle-type between two chucks.
4. a kind of dialysis membrane fixture as claimed in claim 1 is characterized in that: corresponding hole, position is the bar hole of horizontal direction on the lateral wall of said holder and the chuck, is provided with seal groove around the bar hole, and sealing strip is housed in the seal groove.
5. a kind of dialysis membrane fixture as claimed in claim 1, it is characterized in that: the lower end of said holder is provided with at least two feets, and the lower end of feet radially outward is extended with flange, and flange upwards is a wedge that big other end is little in week.
6. a kind of dialysis membrane fixture as claimed in claim 1 is characterized in that: the outer bottom of said holder is equipped with second magnetic agitation.
7. a kind of dialysis membrane fixture as claimed in claim 2, it is characterized in that: said quill can move up and down, and lateral wall is provided with the scale of vertical direction, and the upper end of quill is provided with well and application of sample port lid.
8. a kind of dialysis membrane fixture as claimed in claim 2 is characterized in that: the inwall of said holder is provided with by the position, upper end quill is carried out spacing boss.
9. a kind of dialysis membrane fixture as claimed in claim 2 is characterized in that: said sealing ring is between holder and quill lateral wall and the dead ring, and the upper end inward flange of holder and sealing ring lateral surface are the taper surface that matches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110072365 CN102172476B (en) | 2011-03-24 | 2011-03-24 | Dialysis membrane fixing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110072365 CN102172476B (en) | 2011-03-24 | 2011-03-24 | Dialysis membrane fixing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102172476A CN102172476A (en) | 2011-09-07 |
| CN102172476B true CN102172476B (en) | 2012-12-19 |
Family
ID=44515835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110072365 Expired - Fee Related CN102172476B (en) | 2011-03-24 | 2011-03-24 | Dialysis membrane fixing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102172476B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109046030A (en) * | 2018-09-05 | 2018-12-21 | 东莞市正品五金电子有限公司 | A kind of honeycomb hydrophilic ceramic filter membrane |
| CN110152497B (en) * | 2019-04-04 | 2022-01-04 | 杭州柏医健康科技有限公司 | Permeable membrane preparation device |
| CN111298650A (en) * | 2020-04-01 | 2020-06-19 | 谷昌德 | High-efficient dialysis medical treatment experimental facilities |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5814223B2 (en) * | 1979-02-24 | 1983-03-17 | 株式会社メデクス | Dialysis “filtration” device |
| CN1031245C (en) * | 1990-03-26 | 1996-03-13 | 无锡县红旗超滤设备厂 | Bidirectional ultrafiltration membrane pipe |
| US20100096311A1 (en) * | 2004-10-28 | 2010-04-22 | Nxstage Medical, Inc | Blood treatment dialyzer/filter design to trap entrained air in a fluid circuit |
| CN201179425Y (en) * | 2008-01-18 | 2009-01-14 | 钱峰 | Dialysis desalination plant |
-
2011
- 2011-03-24 CN CN 201110072365 patent/CN102172476B/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN102172476A (en) | 2011-09-07 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121219 Termination date: 20140324 |