CN102159333A - 归一化样品中生物分子含量的方法 - Google Patents

归一化样品中生物分子含量的方法 Download PDF

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Publication number
CN102159333A
CN102159333A CN2009801370183A CN200980137018A CN102159333A CN 102159333 A CN102159333 A CN 102159333A CN 2009801370183 A CN2009801370183 A CN 2009801370183A CN 200980137018 A CN200980137018 A CN 200980137018A CN 102159333 A CN102159333 A CN 102159333A
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China
Prior art keywords
reaction
pcr
sample
reaction vessel
vessel
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Pending
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CN2009801370183A
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English (en)
Chinese (zh)
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C·埃尔巴赫
P·格鲁内费德
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Qiagen GmbH
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Qiagen GmbH
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Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of CN102159333A publication Critical patent/CN102159333A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN2009801370183A 2008-09-17 2009-09-16 归一化样品中生物分子含量的方法 Pending CN102159333A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102008047790.7 2008-09-17
DE102008047790A DE102008047790A1 (de) 2008-09-17 2008-09-17 Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
PCT/EP2009/061990 WO2010031784A1 (de) 2008-09-17 2009-09-16 Verfahren zur normierung des gehalts von biomolekülen in einer probe

Publications (1)

Publication Number Publication Date
CN102159333A true CN102159333A (zh) 2011-08-17

Family

ID=41203765

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801370183A Pending CN102159333A (zh) 2008-09-17 2009-09-16 归一化样品中生物分子含量的方法

Country Status (7)

Country Link
US (1) US20110171656A1 (enExample)
EP (1) EP2326431A1 (enExample)
JP (1) JP2012502642A (enExample)
CN (1) CN102159333A (enExample)
AU (1) AU2009294655B2 (enExample)
DE (1) DE102008047790A1 (enExample)
WO (1) WO2010031784A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101545848B1 (ko) 2012-04-09 2015-08-21 (주)바이오니아 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
CN104217612B (zh) * 2014-07-22 2016-05-25 刘全羽 一种停车场实时智能车辆引导系统
CN104200670B (zh) * 2014-08-26 2016-04-13 长沙理工大学 一种基于车辆地磁感应特征的车辆车型识别方法

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CN1950506A (zh) * 2003-12-30 2007-04-18 新加坡科技研究局 核酸纯化芯片
EP1911844A1 (en) * 2006-10-10 2008-04-16 Qiagen GmbH Methods and kit for isolating nucleic acids

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US20020177144A1 (en) * 1997-12-30 2002-11-28 Jose Remacle Detection and/or quantification method of a target molecule by a binding with a capture molecule fixed on the surface of a disc
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
EP1131159A1 (en) * 1998-11-18 2001-09-12 Orchid BioSciences, Inc. One-step nucleic acid dipstick device with movable membrane
US6423536B1 (en) * 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
AU780319B2 (en) * 1999-08-20 2005-03-17 Promega Corporation Simultaneous isolation and quantitation of DNA
DE19956820A1 (de) * 1999-11-25 2001-05-31 Roche Diagnostics Gmbh Spezies-spezifischer Nachweis von Nukleinsäuren mittels eines Analyseelementes
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JP3602071B2 (ja) * 2001-06-05 2004-12-15 株式会社日立製作所 核酸の精製分離方法
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
DE10201463B4 (de) * 2002-01-16 2005-07-21 Clondiag Chip Technologies Gmbh Reaktionsgefäß zur Durchführung von Array-Verfahren
US20060160085A1 (en) * 2002-11-08 2006-07-20 Timo Hillebrand Novel buffer formulations for isolating purifying and recovering long-chain and short-chain nucleic acids
JP3917595B2 (ja) * 2003-02-26 2007-05-23 株式会社東芝 核酸濃度定量分析チップ、核酸濃度定量分析装置および核酸濃度定量分析方法
DE10322696B3 (de) 2003-05-20 2005-01-20 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zur plasmagestützten Behandlung von vorgebbaren Oberflächenbereichen eines Substrates
WO2004113872A2 (en) * 2003-06-24 2004-12-29 The Trustees Of Columbia University In The City Of New York Covalent methods for immobilization of thiolated biomolecules on siliceous and metallic surfaces
CA2482097C (en) * 2003-10-13 2012-02-21 F. Hoffmann-La Roche Ag Methods for isolating nucleic acids
US20050239104A1 (en) * 2003-11-04 2005-10-27 Ferea Tracy L Microarray controls
US20050142565A1 (en) * 2003-12-30 2005-06-30 Agency For Science, Technology And Research Nucleic acid purification chip
AU2005200670B2 (en) * 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
US20060108225A1 (en) * 2004-11-19 2006-05-25 Stephen Carson High conductivity sieving matrices for high resolution biomolecule separations
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
CN101748117B (zh) * 2005-02-10 2014-03-26 恰根有限公司 反应表面的样品裂解和涂层
EP1690938A1 (de) * 2005-02-11 2006-08-16 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden
DE102005040259A1 (de) * 2005-08-24 2007-03-01 Qiagen Gmbh Verfahren zur Gewinnung von Nukleinsäuren aus Blut
DE102006036536B3 (de) 2006-07-31 2008-02-28 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zum Plasmabehandeln einer Oberfläche
WO2008122310A1 (en) * 2007-04-04 2008-10-16 Chimera Biotec Gmbh Method for the detection of an analyte in biological matrix

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1950506A (zh) * 2003-12-30 2007-04-18 新加坡科技研究局 核酸纯化芯片
WO2006128776A1 (de) * 2005-05-30 2006-12-07 Qiagen Gmbh Vorrichtung und verfahren zur normalisierung von nukleinsäure-konzentrationen
EP1911844A1 (en) * 2006-10-10 2008-04-16 Qiagen GmbH Methods and kit for isolating nucleic acids

Also Published As

Publication number Publication date
AU2009294655B2 (en) 2014-04-17
JP2012502642A (ja) 2012-02-02
WO2010031784A1 (de) 2010-03-25
AU2009294655A1 (en) 2010-03-25
EP2326431A1 (de) 2011-06-01
DE102008047790A1 (de) 2010-04-15
US20110171656A1 (en) 2011-07-14

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Application publication date: 20110817