CN102154169A - Propionibacterium strain and method for producing antibiotic metabolin by virtue of fermentation of same - Google Patents
Propionibacterium strain and method for producing antibiotic metabolin by virtue of fermentation of same Download PDFInfo
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Abstract
The invention relates to a propionibacterium strain sp.CS01 strain and a method for producing antibiotic metabolin by virtue of fermentation of the same. The collection number of the strain is CCTCC (China center for type culture collection) NO:M 2010007. A crude extract obtained by virtue of ammonium sulfate precipitation of the fermented metabolin of the strain has the effect of inhibiting Gram-positive bacteria, partial Gram-negative bacteria and mold. The metabolin can be used for effectively inhibitting the growth of harmful microorganisms polluting food, and prolonging the shelf life of the food, thus the metabolin has an important effect of improving the food safety. The metabolin loses the bacteriostasis function under the action of alkali protease, the relative molecule weight of the metabolin is distributed within a range of 350-3600 Da, and the metabolin is a peptide antibiotic substance.
Description
Technical field
The present invention relates to a kind of microorganism and application thereof, be specifically related to a strain propionibacterium CS01(
PropionibacteriumSp. the method for bacterial strain and the antibiotic metabolite of fermentative production thereof CS01).
Background technology
Propiono-bacterium (
PropionibacteriumSp.) be a class gram-positive microorganism, do not form gemma, do not move, change the different oxygen of energy, anaerobism to oxytolerant, the hydrogen peroxide enzyme positive all contains hemachrome enzyme, is polymorphic existence usually.Propionibacterium comprises 2 big monoids: cutaneous propionibacteria bacillus and dairy products propionibacterium.The former derives from people's skin, is the normal microflora of human skin; The latter separates from soil, vegetables, dairy products and silage and obtains, because therefore its no pathogenicity is widely used in fermentation industry, is mainly used to production Switzerland cheese and production of Propionic Acid by Fermentation Process and VB12.The dairy products propionibacterium comprise propionibacterium freudenreichii (
P.freudenreichii), the Te Shi propionibacterium (
P.thoenii), propionibacterium acide-propionici (
P. acidipropionici) and propionibacterium jensenii (
P. jensenii) etc.
Can synthesize a kind of special peptide or albumen---propionibacterium element in the metabolic process of propionibacterium.The propionibacterium element is the special-shaped antimicrobial substance that is produced by propionibacterium, and its antagonistic property has been subjected to investigator's extensive concern.Prior art has been found that, the propionibacterium bacteriocin that has has bacteriostatic action widely, can suppress gram negative bacterium, part gram-positive microorganism, mould and yeast, and its fungistatic effect is better than significantly that some are commonly used as synthesised food sanitass such as Sodium Benzoate, potassium sorbate, can prolong the shelf-lives of food.Because it is a kind of by biosynthetic natural compounds, advocate today natural, nutritive food the human consumer, the investigator of countries in the world has transferred to the metabolic synthetic preservative that passes through microorganism to the emphasis of research, and promptly the stereoselectivity biocatalysis by complete microorganism cells realizes.Therefore it is significant to screen the production bacterial strain with the higher antimicrobial metabolite of secretion.
Propionibacterium is studied by people abroad very early as probiotic bacterium.As far back as 1986, Grinstead just found that the dairy products propionibacterium suppresses the nearer bacterial growth phenomenon of other relationships, carried out subsequently to the research of this supressor and from
P. thoeniiP126(ATCC 4872) in isolated Jenseniin, and find to add in the yogurt JenseniinG and can make sour milk keep suitable acidity (pH4.2～4.3), this illustrates that this bacteriocin has played vital role to preventing the excessive acidifying of yogurt; 1991, Lyon etc. from
P.thoeniiSeparate among the P127 and obtain Propionicin PLG-1, it is a kind of protein to the proteolytic ferment sensitivity, has thermolability, relative molecular mass is 9327.7 Da, time have activity in pH3～9, it can suppress other kind and some moulds and the yeast of the close propionibacterium of sibship effectively; 2000, Faye etc. from
P. thoeniiIsolating Propionicin T1 in 419 bacterial strains, it all has restraining effect to producing sour propionibacterium, Te Shi propionibacterium, propionibacterium jensenii and Bacterium lacticum, because it has pH tolerance and thermostability, so can be used as the food protection agent, can obtain purifying by ion exchange chromatography, anti-phase and ion-exchange high performance liquid phase; Miescher etc. from
P. jenseniiThe plain Propionicin SM1 of isolating propionibacterium among the DF1 preserved for two weeks in 30 ℃, preserved more than half a year for 4 ℃, still had strong antifungal property, and SM1 be first propose by propionibacterium is plasmid-encoded can be at the bacteriocin of using aspect the genetics; 2003, Galit Ben-Shushart found
P.thoeniiObtain two kinds of different bacillins under the different culture condition of P127; 2004, Merwe etc. from
P. thoeniiIsolated Thoeniicin 447 in 447, it handles 15 min and pH1～10 time cultivation 30 min under 100 ℃ of conditions, still have vigor, can suppress lactobacillus delbruckii and propionibacterium effectively.
Domestic researchist also studies respectively the bacteriostatic activity of propionibacterium growth and metabolite, and the result shows that the propionibacterium metabolite is to zymic bacteriostatic activity difference under the different culture condition.
Summary of the invention
Technical purpose of the present invention provides the new propionibacterium bacterial strain of a strain, makes this bacterial strain have the ability of the antibiotic metabolite of high yield; Another technical purpose of the present invention provides the method for utilizing this propionibacterium strain fermentation to produce antibiotic metabolite.
In order to realize technical purpose of the present invention, technical solution of the present invention is:
One, the present invention with
Propionibacterium feudenreichiiAs1.231 obtains a plant mutant bacterial strain as original starting strain with its mutagenesis and screening, its classification called after propionibacterium CS01(
PropionibacteriumSp. CS01), its preservation registration number is CCTCC NO:M 2010007.
Mutagenesis of the present invention and screening obtain propionibacterium CS01(
PropionibacteriumSp. concrete grammar CS01) is:
1, the cultivation of original strain
With original strain
Propionibacterium feudenreichiiAs1.231 activates on solid medium, the bigger colony inoculation of picking is in liquid seed culture medium, after leaving standstill cultivation 56 h, the centrifugal collection thalline of 8000 r/min under 4 ℃, TrisHCl washed twice with 0.01 M, the pH6.8 that sterilize, be resuspended among the pH6.8,0.01M TrisHCl solution of sterilization, it is standby to obtain bacterium liquid;
2, induction mutation of bacterium
It is the treatment with uv radiation of 15 W after 0.5～5 minute that bacterium liquid is adopted power, photoreactivation 5 h, twice of repetitive operation; Getting 0.2 mL bacterium liquid evenly is applied on the solid medium, put into thick round filter paper then in the culture dish center, nitrosoguanidine 0.02 mL that adds 1 mg/mL is to filter paper, after 30 ℃ of half-lights are cultivated 7 d, colony inoculation around the picking filter paper is to liquid seed culture medium, 30 ℃ leave standstill cultivate 7～8 d after, 4 ℃ of centrifugal 10 minutes of 8000 r/min down, it is standby to get the mutant strain supernatant liquor;
3, superior strain screening
With fermented liquid under 4 ℃, the condition of 8000 r/min centrifugal 10 minutes, in supernatant liquor, slowly add the ammonium sulfate powder while stirring, the ammonium sulfate final concentration is 70%, continue to stir, place to precipitate in 4 ℃ of refrigerators and spend the night, under 4 ℃, the condition of 12000 r/min centrifugal 10 minutes, after throw out adds the less water dissolving, to centrifugal fermented supernatant fluid 48 h that dialyse, change water once with (MWCO:10000Da) dialysis tubing, after the dialysis dialyzate is concentrated about 10 times every 6 h.
Double-deck bacteriostatic experiment substratum is cooled to 50 ℃, earlier pour the bottom substratum into plate, solidifying the back is positioned over the Oxford cup on the bottom substratum earlier, the streptococcus aureus and the Escherichia coli bacteria liquid mixing that add 100 mL in the substratum of upper strata respectively, be mixed with the agar that carries disease germs, be poured on rapidly then on the chilled bottom substratum.Rotating culture dish gently evenly spreads out it, accurately draw 200 mL mutant strains dialysis concentrated solution after cooling successively in Oxford cup (almost being full of hole), covering ware is placed on 4 ℃ and left standstill 2 hours, move to again and cultivate 18 h in 37 ℃ of incubators, use the vernier caliper measurement antibacterial circle diameter, select the bacterial strain of inhibition zone maximum, be about to its called after propionibacterium CS01(
PropionibacteriumSp. CS01).
Two, a kind ofly utilize propionibacterium CS01(of the present invention
PropionibacteriumSp. the method for the antibiotic metabolite of fermentative production CS01).
Its concrete grammar is: with propionibacterium CS01 bacterial classification inoculation to liquid seed culture medium, with 30 ℃ of static cultivation 4 d of temperature, then seed culture fluid is inserted in the fermention medium by 2%～10% inoculum size, pH is 6.0～8.0, leavening temperature is 30～37 ℃, fermentation culture 8～9 d.
Wherein, the inoculum size of the optimum of fermentation process of the present invention is 5%.
Carbon source and nitrogenous source that fermention medium in the fermentation process of the present invention utilizes can as long as can be utilized and transform the antibiotic metabolite of formation by this bacterial strain.In preferred embodiments, the present invention uses glucose, maltose, lactose and Sodium.alpha.-hydroxypropionate as carbon source; Beef extract, yeast extract, peptone, corn steep liquor, urea and ammonium sulfate are nitrogenous source; Further in the preferred embodiment, the present invention uses glucose as carbon source; Yeast extract is a nitrogenous source; Nutrient substance comprises potassium primary phosphate, sal epsom, tween 80.
Most preferred fermention medium is: glucose 25 g/L, yeast extract paste 10 g/L, KH
2PO
41.25 g/L, MgSO
47H
2O 0.2 g/L, tween 80 0.1 mL/L.
The present invention adopts the response surface analysis method to carry out the optimization of strain fermentation condition of the present invention, has determined that processing parameter: pH such as fermentation time, initial pH, temperature and inoculum size are 7.0, and leavening temperature is 30 ℃, and incubation time is 9 d.
Wherein, described fermentation process can also comprise that following purifying obtains the method for antibiotic metabolite: with fermented liquid under 4 ℃, the condition of 8000 r/min centrifugal 10 minutes, slowly add the ammonium sulfate powder in supernatant liquor while stirring, the ammonium sulfate final concentration is 70%, continues to stir, place to precipitate in 4 ℃ of refrigerators and spend the night, under 4 ℃, the condition of 12000 r/min centrifugal 10 minutes, after throw out added water dissolution, 48 h dialysed, change water once every 4 h, at last dialyzate is concentrated 10 times.
Propionibacterium CS01(of the present invention
PropionibacteriumSp. CS01) the protein properties of the antibiotic metabolite that obtains of fermenting determine that method is:
The concentrated solution that fermented liquid is added the ammonium sulfate precipitation thing that the ammonium sulfate powder obtains carries out enzymolysis with proteolytic enzyme (traditional Chinese medicines Shanghai biochemical reagents company), enzymolysis solution contrast concentrated solution carries out bacteriostatic activity and detects, basic protein enzymolysis liquid does not have bacteriostatic activity as a result, papain enzymolysis liquid bacteriostatic activity slightly descends, show that therefore ammonium sulfate precipitation gained bacteriostatics can be had protein properties by protease hydrolysis.The relative molecular mass that adopts high-efficient gel filtration chromatography to measure the dialysis concentrated solution distributes, mainly is distributed in 350Da, 908 Da, the 3600Da scope, and be peptide class antimicrobial substance therefore.
Beneficial effect of the present invention is:
Propionibacterium CS01(of the present invention
PropionibacteriumSp. be propionibacterium bacterial strain common in the dairy products CS01), have the enteron aisle of improvement enteron aisle ecological functions; The peptide class antimicrobial substance that this dissociant produced is a kind of new type natural microbiological antiseptic, and scope of restraining fungi is wider, and bacteriostatic activity is better than the fungistatic effect of self propionic acid that produces; This peptide class sanitas can directly apply in food and the feed.The antibiotic metabolite of gained of the present invention is the wide peptides natural sanitas of a kind of safety, scope of restraining fungi, gram-positive microorganisms such as streptococcus aureus, Shigellae, bacillus cereus, Salmonellas, listeria spp, intestinal bacteria and Gram-negative bacteria all there are restraining effect, particularly mould are had significant inhibitory effect.
Description of drawings
Fig. 1 is the thalli morphology (1000 *) of propionibacterium CS01.
Fig. 2 is the bacteriostatic experiment figure as a result of antibiotic metabolite
The antibacterial figure of 1-mutant strain ammonium sulfate extraction thing among the figure wherein; The antibacterial figure of the former bacterial strain ammonium sulfate extraction of 2-thing; The antibacterial figure of 3-propionic acid; The antibacterial figure of 4-lactic acid; Indicator is a streptococcus aureus.
Fig. 3 is the antibacterial figure of propionibacterium CS01 ammonium sulfate extraction thing to grace radiation Mucor.
Fig. 4 is the relative molecular mass distribution plan of propionibacterium CS01 ammonium sulfate extraction thing.
Microorganism propionibacterium CS01(of the present invention
PropionibacteriumSp. preservation date CS01) is on January 13rd, 2010, and depositary institution's full name is Chinese typical culture collection center, is called for short CCTCC, and its preservation registration number is CCTCC NO:M 2010007.
Embodiment
Embodiment 1
Present embodiment illustrates propionibacterium CS01(of the present invention
PropionibacteriumSp. mutagenic breeding method CS01):
Wherein, the used substratum of present embodiment is:
(1) solid medium (g/L): Sodium.alpha.-hydroxypropionate 20, yeast extract paste 10, KH
2PO
41, MgSO
47H
2O 0.2, agar 20, pH7.0;
(2) liquid seed culture medium (g/L): Sodium.alpha.-hydroxypropionate 20, yeast extract paste 10, KH
2PO
41, MgSO
47H
2O 0.2, pH7.0;
(3) double-deck bacteriostatic experiment substratum (g/L):
The upper strata substratum: extractum carnis 3, peptone 10, NaCl 5, and pH 7.0, agar 7.5;
Bottom substratum: agar 20.
Adopt solid medium with original strain
Propionibacterium feudenreichiiAs1.231(is available from Chinese Academy of Sciences microbial preservation center) activate, the bigger colony inoculation of picking is in liquid seed culture medium, after leaving standstill cultivation 56 h, the centrifugal collection thalline of 8000 r/min under 4 ℃, TrisHCl washed twice with the 0.01M, the pH6.8 that sterilize, be resuspended in the TrisHCl solution of sterilization pH6.8,0.01M, the cell concentration of adjusting bacterium liquid is 10
8Cfu/mL; Get 5 sterilization culture dish, add 15 mL bacterium liquid respectively, culture dish is placed on the magnetic stirring apparatus Stage microscope, turn on agitator, and in ultraviolet lamp (power is 15 W, distance 15 cm) the following irradiation limit stirring of preheating 20 min, and timing.Take out photoreactivation 5 hours respectively at 0.5 min, 1.0 min, 1.5 min, 3.0 min, 5.0 min, repeat aforesaid operations.Get 0.2 mL mutagenesis bacterium liquid respectively, after suitably diluting, evenly be applied on the solid medium, put into thick round filter paper then in the ware center, add 1 mg/mL nitrosoguanidine, 0.02 mL to filter paper, 30 ℃, half-light is cultivated 7d, the colony inoculation around the picking filter paper to liquid (seed) substratum, 30 ℃ leave standstill cultivate 7 d after, 8000 r/min centrifugal 10 minutes under 4 ℃, get supernatant liquor and carry out the mutant strain screening;
Adopt double-deck bacteriostatic experiment substratum screening superior strain.Substratum is cooled to 50 ℃, earlier pour the bottom substratum into plate, solidifying the back is positioned over the Oxford cup on the bottom substratum earlier, the streptococcus aureus (ATCC25923) and intestinal bacteria (ATCC25922) the bacterium liquid mixing that add 100 mL in the substratum of upper strata respectively, be mixed with the agar that carries disease germs, be poured on rapidly then on the chilled bottom substratum.Rotating culture dish gently evenly spreads out it, accurately draw 200 mL mutant strain supernatant liquors after cooling successively in Oxford cup (almost being full of hole), covering the ware lid places 4 ℃ of refrigerators to leave standstill 2 h in advance, move to again and cultivate 18 h in 37 ℃ of incubators, use the vernier caliper measurement antibacterial circle diameter, select the bacterial strain of inhibition zone maximum, promptly obtain mutant strain, be about to its called after propionibacterium CS01(
PropionibacteriumSp. CS01).
Embodiment 2
The present embodiment explanation is in the method for utilizing the antibiotic metabolite of propionibacterium CS01 fermentation fermentative production, to the Optimum of culture medium method.
In order to improve the output of the antibiotic metabolite of mutant strain propionibacterium CS01, adopt Placket-Burman method of design (table 1) to optimize culture condition, thereby obtain mass production conditions.To glucose, yeast extract paste, KH
2PO
4, MgSO
4, factor such as Tween80, fermentation time, temperature, pH and inoculum size is optimized, and investigates the main effect and the interactive one-level effect of each factor.Utilize SAS software to analyze, the result shows that initial glucose concentration, pH and tween 80 are the key factors of the antibiotic metabolite output of influence.Culture condition after the optimization is: glucose 25 g/L, yeast extract paste 10g/L, KH
2PO
41.25g/L, MgSO
47H
2O 0.2g/L, tween 80 0.1mL/L, pH7.0, inoculum size 5%, 30 ℃ of temperature.
Embodiment 3
The present embodiment explanation utilizes propionibacterium CS01(
PropionibacteriumSp. the method for the antibiotic metabolite of fermentative production CS01).
One, fermentation culture:
With propionibacterium CS01 bacterial classification inoculation to liquid seed culture medium, with 30 ℃ of static cultivation 4 d of temperature, then seed culture fluid is inserted in the fermention medium by 2%～10% inoculum size, initial pH is 6.0～8.0, fermenting process pH is controlled at 3.2～6.0, leavening temperature is 30～37 ℃, fermentation culture 8～9 d.
Two, the purifying of antibiotic metabolite
With above-mentioned fermented liquid under 4 ℃, the condition of 8000 r/min centrifugal 10 minutes, in supernatant liquor, slowly add the ammonium sulfate powder while stirring, the ammonium sulfate final concentration is 70%, continue to stir, place to precipitate in 4 ℃ of refrigerators and spend the night, under 4 ℃, the condition of 12000 r/min centrifugal 10 minutes, after throw out adds the less water dissolving, 48 h that dialyse change water once every 4 h, at last dialyzate are concentrated 10 times.
Embodiment 4
The protein properties of antibiotic metabolite that bacterial strain of the present invention produces is determined.With letter Bioisystech Co., Ltd of Alcalase 2.4 L, Neutrase 0.8 L(Novi) and papoid (traditional Chinese medicines Shanghai biochemical reagents company) embodiment 3 described dialysis concentrated solutions are carried out enzymolysis, enzymolysis solution contrast concentrated solution carries out bacteriostatic activity and detects, Alcalase 2.4 L, Neutrase 0.8 L enzymolysis solution bacteriostatic activity disappear as a result, papain enzymolysis liquid bacteriostatic activity slightly descends, show that therefore ammonium sulfate precipitation gained bacteriostatics can be had protein properties by protease hydrolysis; The relative molecular mass of the dialysis concentrated solution that high-efficient gel filtration chromatography (seeing accompanying drawing 4) is measured is distributed in 350Da, 908 Da, the 3600Da scope, therefore
PropionibacteriumSp. to produce antibiotic metabolite be peptide class antimicrobial substance to CS01.
Embodiment 5
The bacteriostatic activity of the antibiotic metabolite of gained of the present invention detects.Test strain is that pathogenic bacterium and spoilage organism important in the food comprises streptococcus aureus (ATCC25923), bacillus cereus (As1.1686), Corynebacterium glutamicum (ATCC 13032), shigella flexneri (51571, China pharmaceutical biological product check institute), Salmonella typhimurium (50115, China pharmaceutical biological product check institute), Listeria monocytogenes (ATCC 19114), intestinal bacteria (ATCC25922), plant lactobacillus (As1.557), lactobacterium casei (As1.62), lactobacillus bulgaricus (As1.148), graceful radiation Mucor (Shanghai industrial microorganism institute), aspergillus niger (As3.739), yeast saccharomyces cerevisiaes (As2.109) etc. separate for this laboratory, identify and preservation.
The preparation of bacterium, saccharomycetes to make fermentation liquid: the test strain access is equipped with in the beef extract-peptone nutrient solution and PDA nutrient solution of 10 mL, cultivates 24 h, be diluted to bacteria suspension with 0.85% physiological saline for 37 ℃ with identical OD value.
Mould spores suspension preparation: the test strain access is equipped with in the potato glucose nutrient solution of 10 mL, cultivates 24 h, be diluted to bacteria suspension with 0.85% physiological saline for 37 ℃ with identical OD value.
Bacteriostatic activity detects: pour the agar that 10 mL have melted in aseptic plate, after solidifying, put into aseptic Oxford cup, 15 mL that melted are detected substratum be cooled to about 50 ℃, add 100 μ L indicator suspensions, fast abundant mixing, pour plate into, treat that it solidifies.In the cup of Oxford, add 200 μ L dialysis concentrated solution, will detect plate and be placed on after 37 ℃ of incubators cultivate 16 h, the measurement antibacterial circle diameter, experimental result sees Table 2.
Table 2 and Fig. 2 and Fig. 3 prove absolutely that the antibiotic metabolite of propionibacterium all has significant inhibitory effect to 4 strain gram positive bacteriums (streptococcus aureus, Listeria monocytogenes, Corynebacterium glutamicum, bacillus cereus), 1 strain gram negative bacterium (intestinal bacteria), 2 strain moulds (aspergillus niger and graceful radiation Mucor) and yeast saccharomyces cerevisiae.
Claims (6)
1. a strain propionibacterium bacterial strain, its classification called after propionibacterium CS01(
PropionibacteriumSp. CS01), its preservation registration number is CCTCC NO:M 2010007.
2. utilize the method for the antibiotic metabolite of claim 1 described propionibacterium CS01 fermentative production, it is characterized in that: with propionibacterium CS01 bacterial classification inoculation to liquid seed culture medium, with 30 ℃ of static cultivation 4 d of temperature, then seed culture fluid is inserted in the fermention medium by 2%～10% inoculum size, fermentation condition is pH6.0～8.0,30～37 ℃ of leavening temperatures, fermentation culture 8～9 d.
3. method according to claim 2, the inoculum size that it is characterized in that described seed culture fluid is 5%.
4. method according to claim 2 is characterized in that described fermention medium is: glucose 25 g/L, yeast extract paste 10 g/L, KH
2PO
41.25 g/L, MgSO
47H
2O 0.2 g/L, tween 80 0.1 mL/L.
5. method according to claim 2 is characterized in that described fermentation condition is that pH is 7.0, and leavening temperature is 30 ℃, and incubation time is 9 d.
6. method according to claim 2, it is characterized in that comprising that also purifying obtains the method for antibiotic metabolite: with fermented liquid under 4 ℃, the condition of 8000 r/min centrifugal 10 minutes, in supernatant liquor, slowly add the ammonium sulfate powder while stirring, the ammonium sulfate final concentration is 70%, continue to stir, place to precipitate in 4 ℃ of refrigerators and spend the night, under 4 ℃, the condition of 12000 r/min centrifugal 10 minutes, after throw out adds water dissolution, 48 h dialyse, change water once every 4 h, at last dialyzate is concentrated 10 times.
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Cited By (4)
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| CN102919551A (en) * | 2012-11-22 | 2013-02-13 | 生物源生物技术（深圳）有限公司 | Method for producing propionic acid type mildewproof agent by utilizing microbial fermentation method, and application thereof |
| CN104830734A (en) * | 2015-05-20 | 2015-08-12 | 常熟理工学院 | Propionibacterium freudenreichii strain and method for fermentatively producing bacteriocin by virtue of propionibacterium freudenreichii strain |
| CN110964668A (en) * | 2019-12-26 | 2020-04-07 | 浙江大学 | Anti-escherichia coli bacillus alvei strain and antiseptic application thereof in cosmetics |
| CN112006066A (en) * | 2019-10-09 | 2020-12-01 | 江苏奕农生物股份有限公司 | Mixed fermentation liquor with improved antibacterial activity and preparation method and application thereof |
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| CN101045910A (en) * | 2007-03-13 | 2007-10-03 | 南京工业大学 | Preparation of propionic acid and co-production of vitamin B by using propionibacterium freudenre NX-412Method (2) |
| CN101445820A (en) * | 2008-10-24 | 2009-06-03 | 上海应用技术学院 | Method for preparing antibacterial metabolin of Propionibacterium and use thereof |
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| CN101045910A (en) * | 2007-03-13 | 2007-10-03 | 南京工业大学 | Preparation of propionic acid and co-production of vitamin B by using propionibacterium freudenre NX-412Method (2) |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919551A (en) * | 2012-11-22 | 2013-02-13 | 生物源生物技术（深圳）有限公司 | Method for producing propionic acid type mildewproof agent by utilizing microbial fermentation method, and application thereof |
| CN104830734A (en) * | 2015-05-20 | 2015-08-12 | 常熟理工学院 | Propionibacterium freudenreichii strain and method for fermentatively producing bacteriocin by virtue of propionibacterium freudenreichii strain |
| CN104830734B (en) * | 2015-05-20 | 2018-05-22 | 常熟理工学院 | The method of one plant of propionibacterium freudenreichii bacterial strain and its fermented-producing bacteria element |
| CN112006066A (en) * | 2019-10-09 | 2020-12-01 | 江苏奕农生物股份有限公司 | Mixed fermentation liquor with improved antibacterial activity and preparation method and application thereof |
| CN110964668A (en) * | 2019-12-26 | 2020-04-07 | 浙江大学 | Anti-escherichia coli bacillus alvei strain and antiseptic application thereof in cosmetics |
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