CN102153190B - Method for rapidly culturing aerobic granular sludge by utilizing self-flocculated spores - Google Patents
Method for rapidly culturing aerobic granular sludge by utilizing self-flocculated spores Download PDFInfo
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- CN102153190B CN102153190B CN2011100535421A CN201110053542A CN102153190B CN 102153190 B CN102153190 B CN 102153190B CN 2011100535421 A CN2011100535421 A CN 2011100535421A CN 201110053542 A CN201110053542 A CN 201110053542A CN 102153190 B CN102153190 B CN 102153190B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Activated Sludge Processes (AREA)
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Abstract
The invention discloses a method for culturing aerobic granular sludge by utilizing self-flocculated spores. The method comprises the following steps of: with high-salt high-concentration organic waste water as a medium culture, adding salt-resistant activated sludge and self-flocculated spore suspension under the condition that sterilization is not carried out, placing the culture medium in a shaker to be cultured for 24-48 hours, and removing supernate, thus the aerobic granular sludge is obtained. Self-flocculated bacterium can form an induction nucleus by self-flocculation under certain hydraulic condition, thallus in the high-salt high-concentration organic waste water grows by taking the induction nucleus as a carrier, and the granular sludge is formed, thus the starting time of the granular sludge can be shortened, the bacterium enriched with waste water body has better effect in waste water treatment, and the aerobic granular sludge cultured by adopting the method disclosed by the invention has the characteristics of regular structure, compactness, uniform size, large specific surface area, strong adsorbability, good precipitation performance and the like.
Description
Technical field
The present invention relates to the treatment process of mud, especially relate to a kind of method of autoflocculation bacterium spore rapidly cultivating aerobic particle sludge.
Background technology
Along with the shortage of industrial expansion and water resources, high salt (more than the salinity 15g/L) waste water that some industrial trade cleaner productions are produced, high salinity waste water are meant that total saliferous (in NaCl content) massfraction is at least 1% waste water.Like saponin waste water, petroleum production wastewater, dyestuff processing waste water, food processing wastewater etc., the pressure that causes to ecotope also strengthens gradually.In existing sewage treatment area, biological treatment is still the treatment process that environmental benefit is arranged most, is widely used.But the high-salt wastewater saltiness is very high, directly gets into Sewage treatment systems and can cause bigger impact to Sewage treatment systems afterwards, because salt concn is too high, microbic activity has been produced restraining effect.
The research of aerobic particle mud is based upon on the anaerobic grain sludge research basis, from MishimaK in 1991 finds aerobic particle mud in aerobic upward flow reactor drum since, has become focus both domestic and external about the research of aerobic particle mud.Be that the aerobic sludge prilling mechanism of inoculum is reached the consistent viewpoint of comparison and is with the ordinary activated sludge: induce under the nuclear situation in nothing, fungi in the seed sludge (thread fungus) is occupied advantage in reactor drum.At initial operating stage, under stronger shear action, thread fungus is that a large amount of breedings of bacterium provide good habitat.Compare with traditional active sludge; Aerobic particle mud has the biological structure rule, than many advantages such as great, that settling property good, biota concentration is high, capacity of resisting impact load is strong; And can greatly improve and water quality, surplus sludge volume is little simultaneously, can reduce or simplify the subsequent treatment process flow process to excess sludge; Reduce the floor space of Sewage treatment systems, reduce cost of investment and running cost.Because the cultivation granule sludge time was longer under nothing was induced and examined; Therefore there is the scholar to adopt anaerobic grain sludge, aerobic particle mud to cultivate granule sludge for inducing nuclear; But anaerobic grain is cultivated the process that aerobic particle mud need pass through granule sludge disintegration regeneration aerobic particle mud, and incubation time is long; And adopt aerobic particle mud to cultivate aerobic particle mud again for inducing nuclear, and then need to cultivate earlier aerobic particle mud, therefore also time-consuming, and still rest on nothing for the cultivation of salt tolerant aerobic particle mud at present and induce the nuclear cultivation stage.At present; Utilize aerobic particle mud to carry out organic chemical contaminant oxygen requirement (chemical oxygen demand; The organic matter degradation of COD) removal, denitrogenation dephosphorizing and difficult degradation etc. all is research focuses of sewage treatment area, has very wide application prospect.
But aerobic particle mud is as an emerging technology, no matter be in experiment or in the middle of practical application; All there is a significant disadvantages; Be exactly that the aerobic particle mud culture condition is harsh, complicated operation, incubation time is long; Propose to add the 30mg/l REE in the patent of invention 200610069502.5 and can shorten the prilling time, Li Xiaoming is published an article and is thought at " bioresource technology ": add 10mg/l Mg
2+Also can shorten the prilling time, also take 7 days, and the aerobic particle mud that cultivation is come out can not reach the organic purpose of removal well under the condition of high density brine waste but lack most.Adopt the artificial synthetic medium in addition in the document mostly, directly do not utilize high salt high-concentration waste water to cultivate the salt tolerant granule sludge, more do not have to adopt the report of inducing nuclear to cultivate the salt tolerant granule sludge.
Summary of the invention
The object of the present invention is to provide a kind of utilize high salt high concentrated organic wastewater add for substratum, in the salt tolerant active sludge autoflocculation bacterium spore as induce nuclear cultivate aerobic particle mud method; Present method can not only shorten the start time of granule sludge; And the bacterium of waste water water body itself has better effect to wastewater treatment, the salt tolerant aerobic particle mud of turning out through present method have regular texture, closely knit, size evenly, characteristics such as big, the high adsorption capacity of specific surface area, sedimentation function be good.
The objective of the invention is to realize like this:
A kind of method of autoflocculation bacterium spore rapidly cultivating aerobic particle sludge, characteristic is: with high salt high concentrated organic wastewater as substratum, under unsterilised condition; Adding mixed liquor suspended solid, MLSS concentration is the salt tolerant active sludge of 10--20g/L, and inoculating spores concentration accounts for the autoflocculation bacterium spore suspension of volume 0.1%--2%, puts into shaking table and cultivates; Culture condition: 140--180 commentaries on classics/min; Temperature 20-30 ℃, cultivate and remove supernatant after 24--48 hour, can obtain aerobic particle mud.
Described autoflocculation bacterium is one or more mixtures in the aspergillus oryzae, terreus, Aspergillus sojae in the aspergillus.
The COD value of said substratum is 20000--43000 mg/L, saltiness is 1%-16%, wherein NO
3 -1Content be 2000--28000mg/L or Cl
-1Content be 1000--26000mg/L, Na
+Content be 2000--40000mg/L or Ca
2+Content be 1000--30000mg/L or Mg
2+Ionic content is 500-20000mg/L.
Because the autoflocculation bacterium is at 160--180 commentaries on classics/min, temperature 20-30 ℃ hydraulics can become the spheroid conduct to induce nuclear by autoflocculation down, and the bacterium in the high salt high concentrated organic wastewater is that carrier is grown to induce nuclear; Form granule sludge; Therefore the present invention as substratum, under unsterilised condition, adds salt tolerant active sludge and autoflocculation bacterium spore suspension with high salt high concentrated organic wastewater; Cultivate, obtain aerobic particle mud.
The present invention has following advantage:
(1), the flocculation thread fungus is that its spore can oneself condense into bead, adopts to add autoflocculation bacterium spore and cultivate granule sludge, culture condition is very simple, has overcome the culture condition of original harshness.
(2), can turn out have regular texture, closely knit, size evenly, the aerobic particle mud of characteristics such as specific surface area is big, (if need material to prove, then this point is not) sedimentation function is good.
(3), utilize the coenosis in the waste water to cultivate granule sludge, can not only shorten the start time of granule sludge, and wastewater treatment had better effect because of the bacterium of enrichment waste water water body itself.
The present invention uses for the actual engineering of aerobic particle mud also can produce very big practical value, particularly aspect the industrial special waste water of handling complicated component.
Embodiment
Below in conjunction with embodiment the present invention is done further explain.
Embodiment 1:
In the Erlenmeyer flask of 500ml, adding the COD value is 20000mg/L, NO
3 -1Content be the waste water 300ml of 20000mg/L, the salt tolerant active sludge and the inoculum size that add mixed liquor suspended solid, MLSS concentration and be 10g/L are the aspergillus oryzae of 3ml, put into shaking table and cultivate; It is 30 ℃ that shaking table is controlled at temperature; Rotating speed 150r/min, natural pH 7.2-7.4 is cultured to 24 hours; Remove supernatant, obtain regular texture, closely knit, size evenly, big, the high adsorption capacity of specific surface area, aerobic particle mud that settling property is good.
Instance 2:
In the Erlenmeyer flask of 500ml, adding the COD value is 43000mg/L, Cl
-1Content be 16000mg/L, Ca
2+Content be the waste water 300ml of 30000mg/L, the salt tolerant active sludge and the inoculum size that add mixed liquor suspended solid, MLSS concentration and be 15g/L are the terreus of 6ml, put into shaking table and cultivate; It is 30 ℃ that shaking table is controlled at temperature; Rotating speed 180r/min, natural pH 7.2-7.4 is cultured to 48 hours; Remove supernatant, obtain regular texture, closely knit, size evenly, big, the high adsorption capacity of specific surface area, aerobic particle mud that settling property is good.
Embodiment 3:
In the Erlenmeyer flask of 500ml, adding the COD value is 20000mg/L, Na
+Content be the waste water 300ml of 20000mg/L, the salt tolerant active sludge and the inoculum size that add mixed liquor suspended solid, MLSS concentration and be 20g/L are the Aspergillus sojae of 1ml, put into shaking table and cultivate; It is 30 ℃ that shaking table is controlled at temperature; Rotating speed 170r/min, natural pH 7.2-7.4 is cultured to 24 hours; Remove supernatant, obtain regular texture, closely knit, size evenly, big, the high adsorption capacity of specific surface area, aerobic particle mud that settling property is good.
Embodiment 4:
In the Erlenmeyer flask of 500ml, adding the COD value is 30000mg/L, Ca
2+Content be the waste water 300ml of 20000mg/L, the salt tolerant active sludge and the inoculum size that add mixed liquor suspended solid, MLSS concentration and be 10g/L are the Aspergillus sojae of 2ml, put into shaking table and cultivate; It is 30 ℃ that shaking table is controlled at temperature; Rotating speed 170r/min, natural pH 7.2-7.4 is cultured to 36 hours; Remove supernatant, obtain regular texture, closely knit, size evenly, big, the high adsorption capacity of specific surface area, aerobic particle mud that settling property is good.
Instance 5:
In the Erlenmeyer flask of 500ml, adding the COD value is 43000mg/L, NO
3 -1Content be 2000mg/L, Mg
2+Ionic content is the waste water 300ml of 500mg/L, and adding mixed liquor suspended solid, MLSS concentration is that salt tolerant active sludge and the inoculum size of 15g/L is the terreus of 6ml, puts into shaking table and cultivates; It is 30 ℃ that shaking table is controlled at temperature; Rotating speed 180r/min, natural pH 7.2-7.4 is cultured to 48 hours; Remove supernatant, obtain regular texture, closely knit, size evenly, big, the high adsorption capacity of specific surface area, aerobic particle mud that settling property is good.
Claims (2)
1. the method for an autoflocculation bacterium spore rapidly cultivating aerobic particle sludge is characterized in that: with high salt high concentrated organic wastewater as substratum, under unsterilised condition; Adding mixed liquor suspended solid, MLSS concentration is the salt tolerant active sludge of 10--20g/L, and inoculating spores concentration accounts for the autoflocculation bacterium spore suspension of volume 0.1%--2%, puts into shaking table and cultivates; Culture condition: 140--180 commentaries on classics/min; 30 ℃ of temperature are cultivated and are removed supernatant after 24--48 hour, can obtain aerobic particle mud; Described autoflocculation bacterium is one or more mixtures in the aspergillus oryzae, terreus, Aspergillus sojae in the aspergillus.
2. the method for autoflocculation bacterium spore rapidly cultivating aerobic particle sludge according to claim 1 is characterized in that: the COD value of said substratum is 20000--43000 mg/L, saltiness is 1%-16%, wherein NO
3 -1Content be 2000--28000mg/L or Cl
-1Content be 1000--26000mg/L, Na
+Content be 2000--40000mg/L or Ca
2+Content be 1000--30000mg/L or Mg
2+Ionic content is 500-20000mg/L.
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| CN2011100535421A CN102153190B (en) | 2011-03-07 | 2011-03-07 | Method for rapidly culturing aerobic granular sludge by utilizing self-flocculated spores |
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| CN102153190B true CN102153190B (en) | 2012-06-06 |
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| CN104609535B (en) * | 2013-11-05 | 2016-05-25 | 中蓝连海设计研究院 | A kind of decanedioic acid waste water that utilizes carries out salt tolerant activated sludge from the method for cultivating |
| CN103739087B (en) * | 2014-01-14 | 2016-04-20 | 复旦大学 | A kind of method of modifying of fungi type biologic grain stibate sorbing material |
| CN103739088B (en) * | 2014-01-14 | 2016-04-20 | 复旦大学 | A kind of method of modifying of bacteria type biologic grain stibate sorbing material |
| CN103755016B (en) * | 2014-01-14 | 2015-10-28 | 复旦大学 | A kind of method of modifying of biological floe sorbing material |
| CN105731636A (en) * | 2014-12-11 | 2016-07-06 | 中国矿业大学 | Method for rapidly cultivating aerobic granular sludge |
| CN110628862A (en) * | 2019-09-24 | 2019-12-31 | 哈尔滨工业大学 | Self-aggregation granular sludge construction method for wastewater gradient energy recovery |
| CN114716007B (en) * | 2022-04-21 | 2023-11-03 | 广东工业大学 | Application of streptomycete in promoting formation of aerobic granular sludge and/or improving stability of aerobic granular sludge |
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| JP2007253106A (en) * | 2006-03-24 | 2007-10-04 | Sumitomo Heavy Ind Ltd | Granular sludge producing method |
| CN101698534B (en) * | 2009-11-12 | 2011-08-31 | 上海交通大学 | Method for optimizing stability of aerobic granular sludge |
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