CN102146428A - Method for extracting collagen II - Google Patents
Method for extracting collagen II Download PDFInfo
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- CN102146428A CN102146428A CN 201010623147 CN201010623147A CN102146428A CN 102146428 A CN102146428 A CN 102146428A CN 201010623147 CN201010623147 CN 201010623147 CN 201010623147 A CN201010623147 A CN 201010623147A CN 102146428 A CN102146428 A CN 102146428A
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- type
- hydrolysis
- cartilage
- animal cartilage
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a method for extracting collagen II. Animal cartilage is subjected to methods of enzymolysis, ultrafiltration, spray drying, and the like to obtain the collagen II. The invention provides a process for comprehensively utilizing the animal cartilage. The method has simple steps and is easy for operation and amplification production. The produced collagen II has the characteristics of high yield and low ash content.
Description
Technical field
The invention belongs to technical field of biochemical industry, especially relate to a kind of extracting method of type.
Background technology
Type is the main collagen protein in the cartilage, the polymer of being made up of three same a chains.Collagen protein in the transmission of iuntercellular information, keep cell phenotype, and the interaction of other matrix and keep aspect such as organized whole's function bringing into play important role.
Type is one of main component that constitutes joint cartilage, to keeping human body proper motion function, joint mobility, buffering body weight and external force the aspects such as pressure in bone and joint is played an important role.Type has to be adjusted immune function of human body, suppresses attack and the destructive function of immunity system to self joint, can treat rheumatic arthritis from the cause of disease, old degeneration osteoarthritis.
The production of type at present generally is to use vacuum concentrator to concentrate the method that adds ethanol sedimentation, can consume a large amount of solvents and energy.
Summary of the invention
The simple effective ways that the objective of the invention is to improve the deficiency of prior art and a kind of extraction of type is provided, present method have the advantages that cost is low, step simple, extract fully, be fit to industrialized production.
The object of the present invention is achieved like this:
Compared with the prior art the present invention has following distinguishing feature and positively effect: the technological method that the present invention relates to is to be raw material with the animal cartilage, adopts enzymolysis, ultrafiltration, spray drying process resulting.This method can obtain the purpose product by the control to reaction times, temperature of reaction and ultra-filtration membrane aperture, is fit to industrialized production.
The type that the present invention produced can be used to produce fields such as medicine, healthcare products, makeup, food.
Method of the present invention is used ultra-filtration technique, can realize concentrating of separate impurities and feed liquid at normal temperatures, and energy consumption is low, and is simple to operate, is convenient to amplify.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1, a kind of extracting method of type, be with nose of an ox bone 500 grams, pulverize the back and add in the 5L tap water, add compound protease 10 grams, hydrolysis 6 hours, filter the clarification back and adopt molecular weight cut-off 8000 daltonian ultrafiltration membrane treatment earlier, see through in the liquid and can obtain type, the ultra-filtration membrane with molecular weight cut-off 1000 will concentrate through liquid again, obtain 24 gram types after the lyophilize.
Embodiment 2, a kind of extracting method of type, be with 1 kilogram of shark suft bone, pulverizing the back adds in the 8L tap water, add 2709 proteolytic enzyme, each 20 gram of AL proteolytic enzyme successively, hydrolysis 4 hours is filtered the clarification back and is used the molecular weight 30000 daltonian ultrafiltration membrane treatment of damming, see through liquid and use molecular weight cut-off 3000 daltonian ultra-filtration membranes to concentrate, obtain 115 gram types after the lyophilize.
Embodiment 3, a kind of extracting method of type, be with 1000 kilograms of pigeon breast cartilages, add in the 6000L tap water, add each 10 kilograms in pancreatin, AL proteolytic enzyme successively, hydrolysis 6 hours is filtered the clarification back and is used the molecular weight 10000 daltonian ultrafiltration membrane treatment of damming, see through liquid and use molecular weight cut-off 1000 daltonian ultra-filtration membranes to concentrate, obtain 61 kilograms of types after the spraying drying.
Claims (2)
1. the extracting method of a type, it is characterized in that with the animal cartilage being raw material, after the animal cartilage hydrolysis, adopt the daltonian ultra-filtration membrane of molecular weight cut-off 8000-30000 to separate the impurity of removing in the collagen protein such as polysaccharide earlier, filtered solution is again with the concentrated type that obtains of the ultra-filtration membrane of 1000-3000.
2. the extracting method of a kind of type according to claim 1, it is characterized in that described animal cartilage hydrolysis is that animal cartilage is added in the cartilage weight 3-10 tap water doubly, the enzyme that adds is a kind of, two kinds and the above any mixing in 2709 proteolytic enzyme, AL proteolytic enzyme, pancreatin, Collagenase or the compound protease, the clarification of hydrolysis after-filtration, concentrated solution after the ultrafiltration promptly gets type after spraying drying or the lyophilize.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010623147 CN102146428A (en) | 2010-12-22 | 2010-12-22 | Method for extracting collagen II |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010623147 CN102146428A (en) | 2010-12-22 | 2010-12-22 | Method for extracting collagen II |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102146428A true CN102146428A (en) | 2011-08-10 |
Family
ID=44420899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010623147 Pending CN102146428A (en) | 2010-12-22 | 2010-12-22 | Method for extracting collagen II |
Country Status (1)
| Country | Link |
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| CN (1) | CN102146428A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352399A (en) * | 2011-10-13 | 2012-02-15 | 桐乡市康源生物制品有限公司 | Extraction method of protolysate |
| CN104087641A (en) * | 2014-07-08 | 2014-10-08 | 天津大学 | Yak skin blood pressure-decreasing collagen polypeptide and preparation method thereof |
| CN114457138A (en) * | 2022-02-25 | 2022-05-10 | 浙江工业大学 | Method for removing type II collagen peptide polysaccharide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1721546A (en) * | 2004-07-12 | 2006-01-18 | 周立军 | Process for preparing II-type collagen compound |
| CN101781672A (en) * | 2010-04-13 | 2010-07-21 | 北京华达杰瑞生物技术有限公司 | Process for preparing hydrolyzed II-type collagen |
-
2010
- 2010-12-22 CN CN 201010623147 patent/CN102146428A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1721546A (en) * | 2004-07-12 | 2006-01-18 | 周立军 | Process for preparing II-type collagen compound |
| CN101781672A (en) * | 2010-04-13 | 2010-07-21 | 北京华达杰瑞生物技术有限公司 | Process for preparing hydrolyzed II-type collagen |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352399A (en) * | 2011-10-13 | 2012-02-15 | 桐乡市康源生物制品有限公司 | Extraction method of protolysate |
| CN104087641A (en) * | 2014-07-08 | 2014-10-08 | 天津大学 | Yak skin blood pressure-decreasing collagen polypeptide and preparation method thereof |
| CN114457138A (en) * | 2022-02-25 | 2022-05-10 | 浙江工业大学 | Method for removing type II collagen peptide polysaccharide |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110810 |