CN102146166B - Method for loading oil soluble additive into bacterial cellulose - Google Patents
Method for loading oil soluble additive into bacterial cellulose Download PDFInfo
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- CN102146166B CN102146166B CN2010106029522A CN201010602952A CN102146166B CN 102146166 B CN102146166 B CN 102146166B CN 2010106029522 A CN2010106029522 A CN 2010106029522A CN 201010602952 A CN201010602952 A CN 201010602952A CN 102146166 B CN102146166 B CN 102146166B
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Abstract
The invention relates to a method for loading an additive into bacterial cellulose, in particular to a method for loading an oil soluble solid and/or liquid additive into the bacterial cellulose. The method for loading the oil soluble solid and/or liquid additive into the bacterial cellulose comprises the following four steps of: (1) preprocessing a vector; (2) preparing oil soluble additive mixed solution; (3) carrying out addition processing on the vector; and (4) cleaning. In the method for loading the oil soluble additive into the bacterial cellulose, the oil soluble additive has strong adhesive force; the loaded oil soluble additive has high stability; the application range is wide; equipment is simple; and the method is suitable for large-scale industrial production and has wide development potential and market prospect.
Description
Technical field
The present invention relates to a kind of oil soluble additive loading method of bacteria cellulose, particularly relate to the solid-state and/or liquid additive of a kind of oil soluble and be written into the inner method of bacteria cellulose.
Background technology
Along with science and technology development and growth in the living standard; People are more and more for the requirement of material various aspects of performance; Intensity and toughness such as for material of construction are had higher requirement; For indexs such as the color of foodstuffs material, taste, mouthfeels comparatively harsh demand or the like has been arranged also; In order to satisfy the growing material and cultural needs of people, the development novel material no doubt is an irreplaceable important channel, and novel additive and advanced additive loading method also are important means that improves and improve the various performances of material.At present, additive mainly comprises multiple chemical industry class additives such as fodder additives, foodstuff additive, concrete additive, oil additives, new chemical additive.Because the restriction of the present technology and equipment of China, the loading method of most of additives all is to adopt the method for primary physical blending the most, promptly under means such as mechanical stirring; Additive is distributed on macroscopic view in the carrier comparatively uniformly, and this method is simple to operate, applied range; Be particularly suitable for improving for the performance or the proterties of liquid or solid powder particulate vector; But most physical blending all is comparatively thick spacious means, and degree of uniformity often rests on the macroscopic aspect, be difficult to reach on the microcosmic evenly; The degree that becomes more meticulous is not enough; Even if reach the comparatively degree of uniformity of microcosmic level by powerful equipment, it is higher to consume energy, and is unfavorable for large-scale industrial production.Also has a kind of method, because the restriction of the performance of material support own causes the physical bond power between carrier and the additive very low; Cause additive from carrier, to spread easily and spin off, and influence additive effect, do not reach the purpose of improving carrier property; Therefore, need show carrier and carry out chemical modification, introduce special functionalization group or particle; Its surface property is made moderate progress, the physics chemical action of raising and additive, this method then is primarily aimed at the solid-state carrier under moisture free dry state; Then can't realize therefore having certain limitation for the easy ruined solid-state carrier of some self dry back structures.
Bacteria cellulose is a natural high moleculer eompound. through long term studies, confirm its chemical structure be by D-Glucopyranose acid anhydride with the linear polymer that β-(1-4)-glycosidic bond links, its chemical formula is C
6H
10O
5, the experiment molecular formula of chemical structure is (C
6H
10O
5) n (n is the polymerization degree); Bacteria cellulose has unique ultra-fine reticular fiber structure, and its width is approximately 30～100nm, and thickness is 3～8nm; Belong to nano-scale fiber; Be the thinnest present natural fiber, so its specific surface area is big, has the specific surface area bigger 200 times than softwood pulp; The ability of hydrogen bonded is strong, bonds inorganic at an easy rate or organic filler and fiber.
Summary of the invention
The oil soluble additive loading method that the purpose of this invention is to provide a kind of bacteria cellulose; Particularly provide a kind of solid-state and/or liquid oil dissolubility additive to be written into the inner method of bacteria cellulose; The oil soluble additive loading method of a kind of bacteria cellulose of the present invention; The strong adhesion of oil soluble additive in the target oil soluble additive carrier that obtains; Be difficult for from carrier, diffusing out, carrier can be applied to various solid-liquid systems, and does not influence the outward appearance and the physico-chemical property of extraneous solid-liquid system.
The oil soluble additive loading method of a kind of bacteria cellulose of the present invention may further comprise the steps:
(1) pre-treatment of bacteria cellulose;
Use zero(ppm) water; Bacteria cellulose is carried out repeatedly soaking and washing;, in 6.0～7.0 scopes, be in the sour environment because work as bacteria cellulose until the pH value inside and outside the bacteria cellulose, a large amount of hydroxyls and the water of the inner fento Shu Hanyou of bacteria cellulose form hydrogen bond; Make fento bundle surface have the water layer structure of one deck or which floor, hindered combining of hydroxyl on oil soluble natural pigment micella particle outside hydrophilic radical and the fento bundle to a certain extent; When bacteria cellulose is in neutral or the weakly alkaline environment; The ability that a large amount of hydroxyl of the inner fento Shu Hanyou of bacteria cellulose and water form hydrogen bond weakens; The water layer on fento bundle surface is destructurized; Promoted combining of hydroxyl on oil soluble natural pigment micella particle outside hydrophilic radical and the fento bundle largely, helped raising and be written into effect.Because most oil soluble additive is relatively more responsive to environment pH value; Acid-fast alkali-proof property is poor; Promptly under acidity or alkaline environment; Oil soluble additive molecule is destroyed easily, thus need make the bacteria cellulose internal pH-values in 6.0～7.0 scopes to avoid oil soluble additive molecule to be destroyed; Then bacteria cellulose is immersed in 1～2h in the edible salt solution of 1～3wt%; Be because the cyberspace structure of the Nano grade of bacteria cellulose and wherein contained a large amount of hydrogen bond; Therefore have very strong suction and water-holding power; In 3% edible salt water, soak the water layer structure that to destroy fiber surface, reduce its inner Zeta potential, help the diffusion and the absorption of oil soluble additive micella particle;
(2) preparation of oil soluble additives mixed liquid;
The oil soluble additive is solved homogeneously in formation oil soluble solution of additive in the organic solvent; Blending emulsifiers and cosurfactant are dissolved in the zero(ppm) water; Then described solution is dispersed in and obtains oil soluble additives mixed liquid in the zero(ppm) water, wherein the oil droplet size of oil soluble solution of additive≤100 nanometers;
Wherein, When described oil soluble additive is the solid-state or liquid oil dissolubility additive of oil soluble; The oil soluble additive is dissolved in the organic solvent with 1: 10～1 ratio; Under the effect of blending emulsifiers and cosurfactant, the oil soluble solution of additive is distributed in the middle of the zero(ppm) water uniformly, form O/W type microemulsion; The mass volume ratio of blending emulsifiers and oil soluble solution of additive is 1: 2～1g/mL; Wherein the consumption of emulsifying agent has important effect to the emulsifying effectiveness of oil soluble additive; Its consumption will account for more than 7.5% of microemulsion total mass, just can obtain comparatively ideal microemulsion of dispersion effect; The volume ratio of oil soluble solution of additive and cosurfactant is 1: 2～1; The volume ratio of oil soluble solution of additive and water is 1: 10～5; The consumption of oil soluble additive can be less, can cause the oil soluble additive concentration in the bacteria cellulose can be lower, can cause dispersion effect poor too much;
(3) interpolation of bacteria cellulose is handled;
In the oil soluble additives mixed liquid that the bacteria cellulose immersion step (2) that step (1) is handled well obtains; The bath raio of bacteria cellulose and oil soluble solution of additive is 1～1.3: 1; The addition of bacteria cellulose should not be very little; Otherwise can not fully utilize the oil soluble solution of additive, so the minimum ratio that should reach 1: 1; Also unsuitablely too much otherwise can make and be written into deleterious, not reach ideal effect, at most surpass 1.3 times of oil soluble solution of additive quality; Under 20 ℃～100 ℃, stir process 0.5～2h takes out; In this treating processes; Time is long more, and the concentration of oil soluble additive is big more in the bacteria cellulose, and the concentration of oil soluble additive does not prolong in time and changes in the bacteria cellulose when reaching peak concentration; So can reach institute's requirement and can stop to handle whenever at a distance from sampling observation test in ten minutes;
(4) cleaning;
Use zero(ppm) water cleaning step (3) to handle the bacteria cellulose that obtains, remove oil soluble additive, promptly obtain the required bacteria cellulose that contains target oil soluble additive attached to the surface.
As optimized technical scheme:
The oil soluble additive loading method of described a kind of bacteria cellulose is characterized in that described organic solvent is an edible oil, comprises safflower oil, walnut oil, peanut oil, VT 18, sweet oil or tea oil; Be flavouring agent perhaps, comprise ETHYLE ACETATE, ethyl n-butyrate, OE or ethyl octylate.
The oil soluble additive loading method of described a kind of bacteria cellulose; It is characterized in that; Described blending emulsifiers is tween 20 and molecule distillating monoglyceride, and mass ratio is 9: 1～4, makes its HLB value near 16, move; Because when the HLB of blending emulsifiers value is 16, the formation of the most suitable O/W type microemulsion.
The oil soluble additive loading method of described a kind of bacteria cellulose is characterized in that described cosurfactant is an ethanol, because the alcoholic acid effect is pretty good and less expensive.
Described oil soluble additive or the foodstuff additive for using always generally comprise vitamin A and Serlabo, vitamins D, vitamin E, vitamin K, Flos Rosae Rugosae quintessence oil or natural pigment.
Wherein, described natural pigment comprises oil soluble natural green pigment, oil soluble natural blue pigment, oil soluble natural yellow pigment, oil soluble natural red colouring matter or the natural purpurin of oil soluble.
Described oil soluble additive or functional food raw material, food supplement raw material, Medicines and Health Product raw material, cosmetics raw material for using always; Mainly comprise the hormone class, like adrenocortical hormone, sexual hormoue, thyroxine, adrenal medullary hormone, pineal hormone, hypothalamic hormone, pituitrin, gastrointestinal hormone `, thyrocalcitonin or prostaglandin(PG).
Described natural plant extracts generally comprises rainbow conk extract polysaccharide peptide, rhabarberone or compound honeysuckle extract.
Described bacteria cellulose is that specification is the square bacterial fibers crude granule of 3mm * 3mm * 3mm; Perhaps 10mm * 10mm * 2mm bacteria cellulose film; Perhaps be 3mm * 3mm * 30m bacteria cellulose bar, the bacteria cellulose ratio under these three kinds of shapes is easier to be written into the oil soluble additive.
Beneficial effect
The oil soluble additive loading method of a kind of bacteria cellulose of the present invention, feasibility is strong, is written into the efficient height; Good uniformity, oil soluble additive strong adhesion, the oil soluble additive that is written into stability is high; Be applicable to being written into of oil soluble additive, applied range, equipment is simple; Easy to operate, suitability for mass industrialized production, what this loading method was directed against is the solid carrier with mesh nanometer space structure; The strong adhesion of additive in the target oil soluble additive carrier that this method obtains is difficult for from carrier, diffusing out, and carrier can be applied to various solid-liquid systems; And do not influence the outward appearance and the physico-chemical property of extraneous solid-liquid system, therefore have big development potentiality and very wide market outlook.
Description of drawings
Fig. 1: the partial sweep Electronic Speculum picture of bacteria cellulose
Fig. 2: bacteria cellulose local structure virtual graph
Fig. 3: the nanometer network gap structure virtual graph of bacteria cellulose
Fig. 4: the oil soluble solution of additive is dispersed in the micella particle of aqueous phase
Fig. 5: the interaction synoptic diagram in the oil soluble additive micella particle diffusion bacteria cellulose nanometer network space
Embodiment
Below in conjunction with embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
The working method of a kind of high color fastness colorful bacterial cellulose provided by the present invention utilizes the cyberspace structure of the Nano grade of bacteria cellulose to reach wherein contained a large amount of hydrogen bond just.The chemical structure of bacteria cellulose has determined its inside to contain a large amount of hydrogen bonds; Therefore have very strong suction and water-holding power; 1～2h in the edible salt solution of 1～3wt%; Can destroy the water layer structure of fiber surface, reduce its inner Zeta potential, help the diffusion and the absorption of additive micella particle; Bacteria cellulose is under the situation of suction fully, and its network space is 50～100nm, and is as shown in Figure 1, and bacteria cellulose microtexture formula is to have a nanometer network space structure by what complicated fento bundle constituted, contains a large amount of hydrogen bonds on its fento bundle; Curve shown in Fig. 2 is the virtual graph of local structure among Fig. 1, and each bar curve is all represented interlaced fento bundle; Fig. 3 is the spacial framework virtual graph of local structure among Fig. 2, the wherein arbitrary network of general reference space, and each bar curve all is the interlaced fento bundle of representative; With of the effect of oil-soluble solution of additive through high pressure homogenizer, blending emulsifiers and cosurfactant, be dispersed in the zero(ppm) water, form the microemulsion of homogeneous; The mean diameter of micella particle wherein is 50nm, and is as shown in Figure 4, and the center ball representes to be dispersed in the spherical oil droplet in the water; The tentacle that is enclosed in the outside is represented surfactant molecule; The oleophilicity group is inserted in the oil droplet, and hydrophilic radical then is exposed in the outside water environment, forms stable micella particle; Bacteria cellulose is joined in the above-mentioned microemulsion; Through heated and stirred; Make the micella particle can be spread in the nanometer network fibrous texture of bacteria cellulose, as shown in Figure 5, wherein contained a large amount of hydrogen bond structure can with the hydrophilic radical generation hydrogen bond action of the emulsifying agent of micella particle surface; Thereby improve the firm degree of additive in bacteria cellulose to a great extent, make additive be difficult for from bacteria cellulose, diffusing out and cause loss.
Embodiment
Below in conjunction with embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
Choose the bacterial fibers crude granule of an amount of 3mm * 3mm * 3mm, through cleaning, making its internal pH-values is 6.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol; Tripoly phosphate sodium STPP, Trisodium Citrate and Tripotassium Citrate join in the zero(ppm) water, and wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 4, and the mass volume ratio of both total masses and oil soluble natural green pigment is 1: 2g/mL; The volume ratio of edible ethanol and oil soluble natural green pigment is 1: 2, and tripoly phosphate sodium STPP, Trisodium Citrate and Tripotassium Citrate three's mass ratio is 1: 2: 2, the mass volume ratio of this three's total mass and oil soluble natural green pigment 1: 40; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Oil soluble natural green pigment is dissolved in the ethyl n-butyrate; The volume ratio of oil soluble natural green pigment and ethyl n-butyrate is 1: 10, and oil soluble natural green cellulose solution and water volume ratio are 1: 20, under the high pressure homogenizer effect, slowly pigment solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacterial fibers crude granule after the adding depickling, the mass ratio of 3 bacterial fibers crude granules and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 2h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain green bacterial fibers crude granule.
Embodiment 2
Choose the bacterial fibers crude granule of an amount of 3mm * 3mm * 3mm, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 3wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol; Tripoly phosphate sodium STPP, Trisodium Citrate join in the zero(ppm) water, and wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 1, and the mass volume ratio of both total masses and oil soluble natural green pigment is 1: 1g/mL; The volume ratio of edible ethanol and oil soluble natural green pigment is 1: 1, and the mass ratio of tripoly phosphate sodium STPP, Trisodium Citrate is 1: 2, the mass volume ratio of the two total mass and oil soluble natural green pigment 1: 100; At normal temperatures, disperseed 30 minutes, it is uniformly dispersed with high pressure homogenizer; Oil soluble natural green pigment is dissolved in the safflower oil; The volume ratio of oil soluble natural green pigment and safflower oil is 1: 1, and oil soluble natural green cellulose solution and water volume ratio are 1: 5, under the high pressure homogenizer effect, slowly pigment solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacterial fibers crude granule after the adding depickling, the mass ratio of bacterial fibers crude granule and treatment solution is 1.3: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain green bacterial fibers crude granule.
Embodiment 3
Choose the bacteria cellulose diaphragm of an amount of 10mm * 10mm * 2mm, through cleaning, making its internal pH-values is 6.0, in the edible salt water of 2wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol; Tripoly phosphate sodium STPP and Tripotassium Citrate join in the zero(ppm) water, and wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 1, and the mass volume ratio of both total masses and oil soluble natural yellow pigment is 1: 1g/mL; The volume ratio of edible ethanol and oil soluble natural yellow pigment is 1: 1, and the mass ratio of tripoly phosphate sodium STPP, Tripotassium Citrate is 1: 2, the mass volume ratio of the two total mass and oil soluble natural yellow pigment 1: 100; At normal temperatures, disperseed 30 minutes, it is uniformly dispersed with high pressure homogenizer; The oil soluble natural yellow pigment is dissolved in the walnut oil; The volume ratio of oil soluble natural yellow pigment and walnut oil is 1: 5, and oil soluble natural yellow cellulose solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly pigment solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose diaphragm after the adding depickling, the mass ratio of bacteria cellulose diaphragm and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 2h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain xanchromatic bacteria cellulose diaphragm.
Embodiment 4
Choose the bacteria cellulose diaphragm of an amount of 10mm * 10mm * 2mm, through cleaning, making its internal pH-values is 6.0, in the edible salt water of 1wt%, soaks 2h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol; Trisodium Citrate and Tripotassium Citrate join in the zero(ppm) water, and wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 4, and the mass volume ratio of both total masses and oil soluble natural green pigment is 1: 2g/mL; The volume ratio of edible ethanol and oil soluble natural green pigment is 1: 2, and Trisodium Citrate and Tripotassium Citrate three's mass ratio is 1: 1, the mass volume ratio of this three's total mass and oil soluble natural green pigment 1: 80; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Oil soluble natural green pigment is dissolved in the sweet oil; The volume ratio of oil soluble natural green pigment and sweet oil is 1: 8, and oil soluble natural green cellulose solution and water volume ratio are 1: 15, under the high pressure homogenizer effect, slowly pigment solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose diaphragm after the adding depickling, the mass ratio of bacteria cellulose diaphragm and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 2h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain green bacteria cellulose diaphragm.
Embodiment 5
Choose the bacteria cellulose bar of an amount of 3mm * 3mm * 30mm, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 3wt%, soaks 3h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and oil soluble natural green pigment is 1: 2g/mL, and the volume ratio of edible ethanol and oil soluble natural green pigment is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Oil soluble natural green pigment is dissolved in the tea oil; The volume ratio of oil soluble natural green pigment and tea oil is 1: 6, and oil soluble natural green cellulose solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly pigment solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain green bacteria cellulose bar.
Embodiment 6
Choose the bacteria cellulose bar of an amount of 3mm * 3mm * 30mm, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 2wt%, soaks 2h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 3: 1, and the mass volume ratio of both total masses and oil soluble natural red colouring matter is 1: 1.5g/mL, and the volume ratio of edible ethanol and oil soluble natural red colouring matter is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; The oil soluble natural red colouring matter is dissolved in the tea oil; The volume ratio of oil soluble natural red colouring matter and tea oil is 1: 5, and natural red cellulose solution of oil soluble and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly pigment solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 2h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain red bacteria cellulose bar.
Embodiment 7
Choose the bacterial fibers crude granule of an amount of 3mm * 3mm * 3mm, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 3wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and oil soluble natural yellow pigment is 1: 1.5g/mL, and the volume ratio of edible ethanol and oil soluble natural yellow pigment is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; The oil soluble natural yellow pigment is dissolved in the tea oil; The volume ratio of oil soluble natural yellow pigment and tea oil is 1: 2, and oil soluble natural yellow cellulose solution and water volume ratio are 1: 5, under the high pressure homogenizer effect, slowly pigment solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacterial fibers crude granule after the adding depickling, the mass ratio of bacterial fibers crude granule and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 2h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain xanchromatic bacterial fibers crude granule.
Embodiment 8
Choose the bacterial fibers crude granule of an amount of 3mm * 3mm * 3mm, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and oil soluble natural blue pigment is 1: 1.5g/mL, and the volume ratio of edible ethanol and oil soluble natural blue pigment is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; The oil soluble natural blue pigment is dissolved in the OE; The volume ratio of oil soluble natural blue pigment and OE is 1: 2, and oil soluble natural blue cellulose solution and water volume ratio are 1: 5, under the high pressure homogenizer effect, slowly pigment solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacterial fibers crude granule after the adding depickling, the mass ratio of bacterial fibers crude granule and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 2h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain blue bacterial fibers crude granule.
Embodiment 9
Choose the bacterial fibers crude granule of an amount of 3mm * 3mm * 3mm, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and the natural purpurin of oil soluble is 1: 1.5g/mL, and the volume ratio of edible ethanol and the natural purpurin of oil soluble is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; The natural purpurin of oil soluble is dissolved in the ethyl octylate; The volume ratio of natural purpurin of oil soluble and ethyl octylate is 1: 2, and natural purpurin solution of oil soluble and water volume ratio are 1: 5, under the high pressure homogenizer effect, slowly pigment solution are joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacterial fibers crude granule after the adding depickling, the mass ratio of bacterial fibers crude granule and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 2h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain the bacterial fibers crude granule of purple.
Embodiment 10
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 2wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and oil soluble natural yellow pigment is 1: 1g/mL, and the volume ratio of edible ethanol and oil soluble natural yellow pigment is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; The oil soluble natural yellow pigment is dissolved in the ETHYLE ACETATE; The volume ratio of oil soluble natural yellow pigment and ETHYLE ACETATE is 1: 1.5, and oil soluble natural yellow cellulose solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly pigment solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain xanchromatic bacteria cellulose bar.
Embodiment 11
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 6.0, in the edible salt water of 3wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and oil soluble Flos Rosae Rugosae quintessence oil is 1: 1g/mL, and the volume ratio of edible ethanol and oil soluble Flos Rosae Rugosae quintessence oil is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; The oil soluble Flos Rosae Rugosae quintessence oil is dissolved in the ETHYLE ACETATE; The volume ratio of oil soluble Flos Rosae Rugosae quintessence oil and ETHYLE ACETATE is 1: 1.5, and Flos Rosae Rugosae quintessence oil solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly oil soluble Flos Rosae Rugosae quintessence oil solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of Flos Rosae Rugosae quintessence oil.
Embodiment 12
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 2h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and oil-soluble vitamine D is 1: 1g/mL, and the volume ratio of edible ethanol and oil-soluble vitamine D is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Oil-soluble vitamine D is dissolved in the safflower oil; The volume ratio of oil-soluble vitamine D and safflower oil is 1: 1.5, and oil-soluble vitamine D solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly oil-soluble vitamine D solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain the bacteria cellulose bar of rich vitamin D.
Embodiment 13
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and oil-soluble vitamine E is 1: 1g/mL, and the volume ratio of edible ethanol and oil-soluble vitamine E is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Oil-soluble vitamine E is dissolved in the ETHYLE ACETATE; The volume ratio of oil-soluble vitamine E and ETHYLE ACETATE is 1: 1.5, and oil-soluble vitamine E and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly oil-soluble vitamine E solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain the bacteria cellulose bar of rich in vitamin E.
Embodiment 14
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and oil-soluble vitamine K is 1: 1g/mL, and the volume ratio of edible ethanol and oil-soluble vitamine K is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Oil-soluble vitamine K is dissolved in the safflower oil; The volume ratio of oil-soluble vitamine K and safflower oil is 1: 1.5, and oil-soluble vitamine K and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly oil-soluble vitamine K solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain the bacteria cellulose bar of rich vitamin K.
Embodiment 15
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 6.0, in the edible salt water of 1wt%, soaks 2h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 7: 2, and the mass volume ratio of both total masses and rainbow conk extract polysaccharide peptide is 1: 1g/mL, and the volume ratio of edible ethanol and rainbow conk extract polysaccharide peptide is 1: 1; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Rainbow conk extract polysaccharide peptide is dissolved in the sweet oil; The volume ratio of rainbow conk extract polysaccharide peptide and sweet oil is 1: 1.5, and rainbow conk extract polysaccharide peptide and water volume ratio are 1: 7, under the high pressure homogenizer effect, slowly oil-soluble vitamine K solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of rainbow conk extract polysaccharide peptide.
Embodiment 16
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 2wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and rhabarberone is 1: 1g/mL, and the volume ratio of edible ethanol and rhabarberone is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Rhabarberone is dissolved in the ETHYLE ACETATE; The volume ratio of rhabarberone and ETHYLE ACETATE is 1: 1.5, and rhabarberone solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly rhabarberone solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of rhabarberone.
Embodiment 17
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 3wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 3: 1, and the mass volume ratio of both total masses and compound honeysuckle extract is 1: 1g/mL, and the volume ratio of edible ethanol and compound honeysuckle extract is 1: 1; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; The compound honeysuckle extract is dissolved in the ETHYLE ACETATE; The volume ratio of compound honeysuckle extract and ETHYLE ACETATE is 1: 1.5, and compound honeysuckle extract solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly the compound honeysuckle extract solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of compound honeysuckle extract.
Embodiment 18
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 3wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and adrenocortical hormone is 1: 1g/mL, and the volume ratio of edible ethanol and adrenocortical hormone is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Adrenocortical hormone is dissolved in the ETHYLE ACETATE; The volume ratio of adrenocortical hormone and ETHYLE ACETATE is 1: 1.5, and adrenocortical hormone solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly adrenocortical hormone solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of adrenocortical hormone.
Embodiment 19
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 2wt%, soaks 2h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and both total masses and thyroxinic mass volume ratio are 1: 1g/mL, and edible ethanol and thyroxinic volume ratio are 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Thyroxine is dissolved in the ETHYLE ACETATE, and the volume ratio of thyroxine and ETHYLE ACETATE is 1: 1.5, and thyroxine solution and water volume ratio are 1: 10; Under the high pressure homogenizer effect, slowly thyroxine solution is joined in the above-mentioned solution, continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in thyroxinic bacteria cellulose bar.
Embodiment 20
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 8: 3, and the mass volume ratio of both total masses and adrenal medullary hormone is 1: 1g/mL, and the volume ratio of edible ethanol and adrenal medullary hormone is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Adrenal medullary hormone is dissolved in the ETHYLE ACETATE; The volume ratio of adrenal medullary hormone and ETHYLE ACETATE is 1: 1.5, and adrenal medullary hormone solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly adrenal medullary hormone solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of adrenal medullary hormone.
Embodiment 21
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and pineal hormone is 1: 1g/mL, and the volume ratio of edible ethanol and pineal hormone is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Pineal hormone is dissolved in the ETHYLE ACETATE; The volume ratio of pineal hormone and ETHYLE ACETATE is 1: 2, and pineal hormone solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly pineal hormone solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of pineal hormone.
Embodiment 22
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and hypothalamic hormone is 1: 1g/mL, and the volume ratio of edible ethanol and hypothalamic hormone is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Hypothalamic hormone is dissolved in the ETHYLE ACETATE; The volume ratio of hypothalamic hormone and ETHYLE ACETATE is 1: 1, and hypothalamic hormone solution and water volume ratio are 1: 10, under the high pressure homogenizer effect, slowly hypothalamic hormone solution is joined in the above-mentioned solution; Continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of hypothalamic hormone.
Embodiment 23
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 6.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and pituitrin is 1: 1g/mL, and the volume ratio of edible ethanol and pituitrin is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Pituitrin is dissolved in the ETHYLE ACETATE, and the volume ratio of pituitrin and ETHYLE ACETATE is 1: 2, and pituitrin solution and water volume ratio are 1: 10; Under the high pressure homogenizer effect, slowly pituitrin solution is joined in the above-mentioned solution, continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of pituitrin.
Embodiment 24
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and thyrocalcitonin is 1: 1g/mL, and the volume ratio of edible ethanol and thyrocalcitonin is 1: 1; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Thyrocalcitonin is dissolved in the ETHYLE ACETATE, and the volume ratio of thyrocalcitonin and ETHYLE ACETATE is 1: 1.5, and thyrocalcitonin solution and water volume ratio are 1: 10; Under the high pressure homogenizer effect, slowly thyrocalcitonin solution is joined in the above-mentioned solution, continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of thyrocalcitonin.
Embodiment 25
Choose an amount of 3mm * 3mm * 30mm bacteria cellulose bar, through cleaning, making its internal pH-values is 7.0, in the edible salt water of 1wt%, soaks 1h then, for use; With tween 20, molecule distillating monoglyceride, edible ethanol join in the zero(ppm) water; Wherein the mass ratio of tween 20 and molecule distillating monoglyceride is 9: 2, and the mass volume ratio of both total masses and prostaglandin(PG) is 1: 1g/mL, and the volume ratio of edible ethanol and prostaglandin(PG) is 1: 2; At normal temperatures, disperseed 15 minutes, it is uniformly dispersed with high pressure homogenizer; Prostaglandin(PG) is dissolved in the ETHYLE ACETATE, and the volume ratio of prostaglandin(PG) and ETHYLE ACETATE is 1: 1.5, and prostaglandin(PG) solution and water volume ratio are 1: 10; Under the high pressure homogenizer effect, slowly prostaglandin(PG) solution is joined in the above-mentioned solution, continue to disperse 30min, obtain the stable microemulsion treatment solution.The micro emulsion treatment solution is transferred in the agitator kettle, the bacteria cellulose bar after the adding depickling, the mass ratio of bacteria cellulose bar and treatment solution is 1: 1, after stirring, at normal temperatures, continues to stir 1h, takes out; To be cooled to room temperature, vacuumize with the polypropylene plastics pocket and to pack, be put in shady and cool place after the sterilization and preserve and use.Promptly obtain being rich in the bacteria cellulose bar of prostaglandin(PG).
Claims (6)
1. the oil soluble additive loading method of a bacteria cellulose is characterized in that may further comprise the steps:
(1) pre-treatment of bacteria cellulose;
Use zero(ppm) water, bacteria cellulose carried out repeatedly soaking and washing, until the pH value inside and outside the bacteria cellulose in 6.0～7.0 scopes; Be immersed in 1～2h in the edible salt solution of 1～3wt% then, take out bacteria cellulose;
(2) preparation of oil soluble additives mixed liquid;
The oil soluble additive is solved homogeneously in formation oil soluble solution of additive in the organic solvent; Blending emulsifiers and cosurfactant are dissolved in the zero(ppm) water; Then described solution is dispersed in and obtains oil soluble additives mixed liquid in the zero(ppm) water, wherein the oil droplet size of oil soluble solution of additive≤100 nanometers;
Wherein, Described oil soluble additive is the solid-state or liquid additive of oil soluble; The oil soluble additive is dissolved in the organic solvent with 1: 10～1 mass ratio; Under the effect of blending emulsifiers and cosurfactant, the oil soluble solution of additive is distributed in the middle of the zero(ppm) water uniformly, form O/W type microemulsion; The mass volume ratio of blending emulsifiers and oil soluble solution of additive is 1: 2～1g/mL, and the volume ratio of oil soluble solution of additive and cosurfactant is 1: 2～1, and the volume ratio of oil soluble solution of additive and water is 1: 10～5;
(3) interpolation of bacteria cellulose is handled;
In the oil soluble additives mixed liquid that the bacteria cellulose immersion step (2) that step (1) is handled well obtains; The bath raio of bacteria cellulose and oil soluble solution of additive is 1～1.3: 1; Under 20 ℃～100 ℃, stir process 0.5～2h takes out the bacteria cellulose that adds after handling;
(4) cleaning;
Use zero(ppm) water to clean the bacteria cellulose that adds after handling, remove oil soluble additive, promptly obtain the required bacteria cellulose that contains target oil soluble additive attached to the surface.
2. the oil soluble additive loading method of a kind of bacteria cellulose as claimed in claim 1 is characterized in that, described oil soluble additive is oil soluble foodstuff additive, oil soluble natural animal-plant extract or oil soluble Medicines and Health Product raw material commonly used.
3. the oil soluble additive loading method of a kind of bacteria cellulose as claimed in claim 1 is characterized in that described organic solvent is an edible oil, is safflower oil, walnut oil, peanut oil, VT 18, sweet oil or tea oil; Perhaps described organic solvent is a flavouring agent, is ETHYLE ACETATE, ethyl n-butyrate, OE or ethyl octylate.
4. the oil soluble additive loading method of a kind of bacteria cellulose as claimed in claim 1 is characterized in that, described blending emulsifiers is tween 20 and molecule distillating monoglyceride, and mass ratio is 9: 1～4.
5. the oil soluble additive loading method of a kind of bacteria cellulose as claimed in claim 1 is characterized in that, described cosurfactant is an ethanol.
6. the oil soluble additive loading method of a kind of bacteria cellulose as claimed in claim 1 is characterized in that, described bacteria cellulose is a square bacterial fibers crude granule, perhaps is the bacteria cellulose diaphragm, perhaps is the bacteria cellulose bar.
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| CN110029443B (en) * | 2019-05-24 | 2022-02-08 | 华南协同创新研究院 | Application of camellia oil fiber membrane material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DD242822A1 (en) * | 1985-11-19 | 1987-02-11 | Akad Wissenschaften Ddr | PROCESS FOR IMMOBILIZING BIOLOGICAL MATERIAL |
| EP1660670A1 (en) * | 2003-07-03 | 2006-05-31 | Politechnika Lodzka | A method for the production of bacterial cellulose |
| JP2010235802A (en) * | 2009-03-31 | 2010-10-21 | Agri Bioindustry:Kk | Support for conductive material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DD242822A1 (en) * | 1985-11-19 | 1987-02-11 | Akad Wissenschaften Ddr | PROCESS FOR IMMOBILIZING BIOLOGICAL MATERIAL |
| EP1660670A1 (en) * | 2003-07-03 | 2006-05-31 | Politechnika Lodzka | A method for the production of bacterial cellulose |
| JP2010235802A (en) * | 2009-03-31 | 2010-10-21 | Agri Bioindustry:Kk | Support for conductive material |
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