CN102144767B

CN102144767B - Method for extracting rebaudiodside A (RA) with content of 80%

Info

Publication number: CN102144767B
Application number: CN201010108135A
Authority: CN
Inventors: 张来旺; 孔祥生; 来雨强
Original assignee: SAINT-OUEN SHANDONG PHARMACEUTICAL Co Ltd
Current assignee: SAINT-OUEN SHANDONG PHARMACEUTICAL Co Ltd
Priority date: 2010-02-10
Filing date: 2010-02-10
Publication date: 2012-10-10
Anticipated expiration: 2030-02-10
Also published as: CN102144767A

Abstract

The invention discloses a method for extracting rebaudiodside A (RA) with a content of 80%, belonging to the technical field of food additive production. The RA is a component with the best taste in stevioside; and due to different processes and methods, the traditional production processes generally have the problems of low conversion rate, high energy consumption, high cost, unstable control of effective constituents and the like. The method provided by the invention comprises the steps of: mixing solvents such as methanol and the like according to a certain proportion; then mixing the mixed solvents with RA with low purity according to a certain proportion; carrying out solid-liquid separation after dissolving and agitating; and drying or washing and then drying obtained solid to obtain RA with high purity, wherein the content of the RA can be up to more than 80% and the conversion rate of the RA is up to more than 80%. The method for producing the RA with high purity has the advantages of simple process, low energy consumption, stable RA purity, no lingering bitterness and the like and is suitable for industrialized production.

Description

The process for extracting of a kind of 80% content Lai Baodi glycosides A

Technical field

The invention belongs to the foodstuff additive production technical field.

Background technology

Steviosides is that a kind of high sugariness that extraction obtains from sweet Stevia is hanged down heat energy, flavor matter is good and the natural low-heat sugar source of safety non-toxic, and its sugariness is 150～300 times of sucrose, can substitute asccharin or sucrose, is applied in various food, the beverage.Steviosides can also prevent diseases such as mellitus, obesity and pediatric saprodontia, is patient's desirable sweeting agent.But the bitter taste of stevioside influences its popularization and utilization always, and Japan and Korea S adopt the method for transferring enzyme and lytic enzymeization to improve the bitter aftertaste of stevioside; Insufficient is the spinoff sugariness multiple decline often that brings, cost up.Cause the difficulty in the application equally.But progressively increase along with the natural sweeteners market requirement; The back bitter taste that more and more enterprises begins to pay close attention to research and solves stevia rebaudianum; People attempt the stevia rebaudianum product of refining refinement Gao Laibaodi glycosides content; Get rid of bad composition steviobioside of mouthfeel and Du Ke glucoside, sugariness can up to 350-400 doubly have been removed the back bitter taste of stevia rebaudianum simultaneously like this.At present, some enterprises of Korea S, Malaysia, the U.S., China etc. can be purified to the Lai Baodi glycosides more than 80%, but because the difference of technology and method separately; The ubiquity transformation efficiency is low; Energy consumption is high, and cost is high, drawbacks such as effective constituent control instability.

Summary of the invention

In order to overcome the above problems; The invention provides the process for extracting of a kind of production 80% content Lai Baodi glycosides A, this method operational path is simple, and is not high to equipment requirements; And have advantages such as less energy-consumption, high production, stable, the thorough removal of RA purity back bitter taste, be fit to suitability for industrialized production.Concrete technical scheme is following, the process for extracting of a kind of production 80% content Lai Baodi glycosides A, and it comprises following step:

(1) the solvent preparatory stage is methyl alcohol with methyl alcohol, water in the ratio of solvent: water (6～10: 0～4).

(2) mix stages: the low-purity stevioside was mixed than 1: 3～1: 15 by plastid with the methanol mixed solvent.

(3) agitation phases:, and keep 1～4h with the said mixture stirring and dissolving.

(4) the placement stage: will stir the back mixture and fully separate out at the placement of the temperature below 30 ℃ 0～24h RA.

(5) solid-liquid separation: carry out solid-liquid separation with centrifugal or filtering method, filter that aperture of filter material is less than 50 μ m or greater than 500 orders.

(6) the liquid separated thing is reclaimed 1～4h in boiling temperature, should be at 30 ℃ with held 0～96h.

(7) mixed solution is placed to separate out and is finished the back and carry out solid-liquid separation with centrifugal or filtering method, and the filtration aperture of filter material is less than 50 μ m or more than 500 orders.

(8) method of isolating solid substance being done with oven dry or spray is carried out drying.

Embodiment

Embodiment 1

(1) the solvent preparatory stage is methyl alcohol with methyl alcohol, water in the ratio of solvent: water (9: 1).

(2) mix stages: the low-purity stevioside was mixed than 1: 15 by plastid with the methanol mixed solvent.

(3) agitation phases:, and keep 1h with the said mixture stirring and dissolving.

(4) the placement stage: will stir the back mixture and place 6h in the temperature below 30 ℃.

(5) solid-liquid separation: carry out solid-liquid separation with filtering method, filter filter material 500 purpose filter clothes.

(6) the liquid separated thing is reclaimed 6h in boiling temperature, reclaim liquid and should fully separate out with held 72h A at 30 ℃.

(7) carry out solid-liquid separation with filtering method, filter filter material 500 purpose filter clothes.

(8) isolating solid substance is dried with infrared drying oven.

Embodiment 2

(1) the solvent preparatory stage is methyl alcohol with methyl alcohol, water in the ratio of solvent: water (7: 3).

(2) mix stages: the low-purity stevioside was mixed than 1: 6 by plastid with the methanol mixed solvent.

(3) agitation phases:, and keep 2h with the said mixture stirring and dissolving.

(4) the placement stage: will stir the back mixture and place 12h in the temperature below 30 ℃.

(6) the liquid separated thing is reclaimed 3h in boiling temperature, reclaim liquid and should fully separate out with held 48h A at 15 ℃.

(8) with behind the isolating solid substance deionized water dissolving, volatilize organic solvent, the dried machine of spray-drier is dry.

Claims

1. the process for extracting of a content Lai Baodi glycosides A is characterized in that it comprises following step:

(1) the solvent preparatory stage: is to prepare at 6～10: 0～4 methyl alcohol and water according to the ratio of solvent;

(2) mix stages: the low-purity stevioside was mixed than 1: 3～1: 15 by plastid with the methanol mixed solvent;

(3) agitation phases:, and keep 1～4h with the said mixture stirring and dissolving;

(4) the placement stage: will stir the back mixture and place 6～24h in the temperature below 30 ℃, Lai Baodi glycosides A fully separates out;

(5) solid-liquid separation: carry out solid-liquid separation with centrifugal or filtering method, filter aperture of filter material greater than 500 orders;

(6) the liquid separated thing is reclaimed 1～4h in boiling temperature, at 30 ℃ with held 48～96h;

(7) after mixed solution is placed and separated out end, carry out solid-liquid separation, filter aperture of filter material greater than 500 orders with centrifugal or filtering method;

(8) the method drying of isolating solid substance being done with oven dry or spray.