CN102116712B - Fully-automatic immune tissue chemical dyeing instrument - Google Patents
Fully-automatic immune tissue chemical dyeing instrument Download PDFInfo
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- CN102116712B CN102116712B CN2011100334494A CN201110033449A CN102116712B CN 102116712 B CN102116712 B CN 102116712B CN 2011100334494 A CN2011100334494 A CN 2011100334494A CN 201110033449 A CN201110033449 A CN 201110033449A CN 102116712 B CN102116712 B CN 102116712B
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- pipe
- antibody
- screw mandrel
- displacement
- motor
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- 238000004043 dyeing Methods 0.000 title abstract description 8
- 239000000126 substance Substances 0.000 title abstract 6
- 238000006073 displacement reaction Methods 0.000 claims abstract description 100
- 238000010438 heat treatment Methods 0.000 claims abstract description 33
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 239000004065 semiconductor Substances 0.000 claims abstract description 16
- 238000011532 immunohistochemical staining Methods 0.000 claims description 10
- 230000001360 synchronised effect Effects 0.000 claims description 8
- 238000005070 sampling Methods 0.000 claims description 7
- 125000006850 spacer group Chemical group 0.000 claims description 7
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000003118 histopathologic effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
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Abstract
The invention relates to a fully-automatic immune tissue chemical dyeing instrument which aims at the problems that the present immune tissue chemical dyeing experiment device generally adopts manual operation and has poor efficiency and low accuracy. An antibody holding device, a pipe taking and liquid filling device and a temperature control and heating device are arranged on a base of the fully-automatic immune tissue chemical dyeing instrument. The antibody holding device comprises an antibody pipe and a pipe support; screw threads are formed at the upper part of the lateral wall of a pipe body of the antibody body; and a piston is arranged in the pipe body. The pipe taking and liquid filling device comprises a transverse displacement device, a longitudinal displacement device and a vertical displacement device, wherein a sampler in the vertical displacement device is cylindrical, and screw threads matched with the lateral wall of the antibody pipe are formed on the inner wall of the sampler. The temperature control and heating device comprises a rack, a motor, a movable plate, a slice rack, a water channel and semiconductor heating plates; and each semiconductor heating plate corresponds to each slice in position. The fully-automatic immune tissue chemical dyeing instrument realizes the fully-automatic immune tissue chemical dyeing experiment and improves the experiment efficiency and the experiment precision without manual operation.
Description
Technical field
The invention belongs to the checkout equipment technical field, what be specifically related to is the full-automatic immunohistochemical staining appearance that is applied in the histopathologic slide of hospital.
Background technology
The laboratory method of " immunohistochemical staining " is a very rigorous laboratory work, generally uses instrument and at home owing to receive the expensive influence of import instrument in developed country, and present 99% is manual operations with hospital; The problem of manual main existence is: 1. consuming time: dyeing each time needs 2. easy error more than 4-5 consuming time hour: because the used antibody of each " immunohistochemical staining " and reagent type reach tens of kinds of manual works add the easy mistake of reagent add or forget add, the reaction time can not effectively control, and 3. can not accomplish standardization and standardized laboratory room managing.
Summary of the invention
The object of the invention is exactly the deficiency to prior art, and a kind of full-automatic immunohistochemical staining appearance is provided.
The present invention includes base, base is provided with the antibody apparatus for placing, gets pipe liquid-adding device and temperature control heating arrangement.
Described antibody apparatus for placing comprises antibody pipe and pipe support;
The antibody pipe comprises body, and the lower end of body is connected with the liquid feeding head, and the liquid feeding headgear has headgear; Be provided with piston in the body, piston and pull bar are through being threaded, and the top of the sidewall of body has screw thread, and the sidewall of body is provided with spacer pin and scale mark;
Pipe support comprises platform and the platform support vertical with platform, has a plurality of antibody pipe sockets on the platform, and a plurality of antibody pipe sockets are arranged; The edge of each antibody pipe socket has stopper slot, and each antibody pipe socket limit has the headgear socket, and a plurality of headgear sockets are arranged.The stopper slot of pipe support is corresponding with the spacer pin shape, after the antibody pipe inserts antibody pipe socket, guarantees that body can not rotate.
The described pipe liquid-adding device of getting comprises transversal displacement device, length travel device and vertical displacement devices;
The transversal displacement device comprises the transversal displacement motor that is provided with on mobile base and the mobile base; Transversal displacement screw mandrel and transversal displacement slide bar laterally arrange, and transversal displacement screw mandrel and transversal displacement motor are connected;
The length travel device comprises length travel screw mandrel and the length travel slide bar that laterally arranges, and an end and the length travel motor of length travel screw mandrel are connected, and the other end is threaded with the mobile base of transversal displacement device;
Vertical displacement devices comprises support, gets pipe motor, perpendicular displacement motor, liquid feeding bar, perpendicular displacement screw mandrel and ST, and support is connected with the transversal displacement wire rod thread; The top of support is set with the perpendicular displacement motor, the bottom is provided with the ST seat, gets the pipe motor and is fixedly installed on the ST seat; Perpendicular displacement screw mandrel, liquid feeding bar and perpendicular displacement slide bar laterally arrange, and an end of liquid feeding bar is fixedly connected with slide block, and the other end of liquid feeding bar is provided with ST and photoelectric sensor, and the liquid feeding bar passes the setting of ST seat;
Described perpendicular displacement screw mandrel and perpendicular displacement slide bar all pass the slide block setting; The lower end of perpendicular displacement slide bar is fixedly connected with the ST seat; Perpendicular displacement slide bar and slide block are slidingly matched, and the perpendicular displacement screw mandrel is threaded with slide block, and the perpendicular displacement slide bar is provided with register pin; One end of perpendicular displacement screw mandrel and the clutch end of perpendicular displacement motor are provided with the perpendicular displacement belt wheel, and two perpendicular displacement belt wheels connect through belt; The clutch end of getting the pipe motor is provided with the sampling belt wheel, and the sampling belt wheel is connected through belt with ST; Described ST is a tubular, and inwall is attacked the screw thread with the sidewall coupling of the body of antibody pipe;
Described transversal displacement screw mandrel is vertical with the length travel screw mandrel, the transversal displacement screw mandrel is all vertical with the perpendicular displacement screw mandrel with the length travel screw mandrel.
Described temperature control heating arrangement comprises frame, motor, portable plate, section frame, tank, semiconductor heating plate; The clutch end of motor is connected with the power wheel that is arranged on the frame;
Portable plate is arranged on frame top, and the portable plate symmetrical, parallel is set with two rhizoid bars, and two rhizoid bars pass the frame setting respectively, and respectively be arranged on frame on two synchronous pulleys be threaded; Two synchronous pulleys are connected through belt with power wheel, realize rotating synchronously;
The section frame is connected through cramp bar with portable plate, and section is set up and is equipped with row's draw-in groove, and section is inserted in the draw-in groove; Tank is arranged between section frame and the portable plate, and heating frame is arranged on the tank, and the semiconductor heating plate is arranged on the heating frame, and every chip semiconductor heating plate is corresponding with every slice position on the frame of cutting into slices, and every chip semiconductor heating plate is provided with temperature control sensor.
Immunohistochemical staining appearance of the present invention has realized that immunohistochemical staining experiment automatically carries out, and need not improve conventional efficient on the one hand through manual operations, has improved experimental precision on the other hand.
Description of drawings
Fig. 1 is a structural representation of the present invention;
Fig. 2-1 is the structural representation of antibody apparatus for placing antibody pipe among Fig. 1;
Fig. 2-2 is the structural representation of the pipe support of antibody apparatus for placing among Fig. 1;
Fig. 3 is for getting the structural representation of pipe liquid-adding device among Fig. 1;
Fig. 4 is the structural representation of temperature control heating arrangement among Fig. 1.
Embodiment
As shown in Figure 1, full-automatic immunohistochemical staining appearance comprises base 4, and base 4 is provided with antibody apparatus for placing 1, gets pipe liquid-adding device 2 and temperature control heating arrangement 3.
Antibody apparatus for placing 1 comprises antibody pipe and pipe support.
Shown in Fig. 2-1, the antibody pipe comprises body 1-5, and the lower end of body 1-5 is connected with liquid feeding head 1-6, and liquid feeding head 1-6 is with headgear 1-7.Be provided with piston 1-2 in the body 1-5, piston 1-2 and pull bar 1-1 are through being threaded, and the top of the sidewall of body 1-5 has screw thread 1-3, and the sidewall of body 1-5 is provided with spacer pin 1-4 and scale mark.
Shown in Fig. 2-2, pipe support comprises platform 1-8 and the platform support 1-12 vertical with platform, has a plurality of antibody pipe socket 1-9 on the platform 1-8, and a plurality of antibody pipe socket 1-9 are arranged; The edge of each antibody pipe socket 1-9 has stopper slot 1-10, and each antibody pipe socket 1-9 limit has headgear socket 1-11, and a plurality of headgear socket 1-11 are arranged.The stopper slot 1-10 of pipe support is corresponding with the spacer pin 1-4 shape of antibody pipe, after the antibody pipe inserts antibody pipe socket, guarantees that antibody Guan Buhui rotates.
In the work, at first pull bar 1-1 is connected on the piston 1-2, extracts antibody, screw off pull bar 1-1 then, body 1-5 is inserted in the antibody pipe socket 1-9 of pipe support, spacer pin 1-4 pegs graft in the stopper slot 1-10 simultaneously during insertion.If headgear 1-7 is taken off in sampling, insert in the headgear socket 1-11 on this antibody pipe socket 1-9 limit, guarantee the corresponding antibody pipe 1 of each headgear 1-7, avoid cross pollution.Utilize the sampler of dyeing instrument to utilize the screw thread 1-3 rotation of body 1-5 sidewall to take out that the antibody pipe is taken a sample, application of sample then.
As shown in Figure 3, get pipe liquid-adding device 2 and comprise transversal displacement device, length travel device and vertical displacement devices.
The transversal displacement device comprises that mobile base 2-3 and mobile base 2-3 go up the transversal displacement motor 2-4 that is provided with; Transversal displacement screw mandrel 2-11 and transversal displacement slide bar 2-8 laterally arrange, and transversal displacement screw mandrel 2-11 and transversal displacement motor 2-4 are connected.
The length travel device comprises length travel screw mandrel 2-17 and the length travel slide bar 2-2 that laterally arranges, and the end of length travel screw mandrel 2-17 and length travel motor 2-1 are connected, and the other end is threaded with the mobile base 2-3 of transversal displacement device.
Vertical displacement devices comprises support 2-5, gets pipe motor 2-16, perpendicular displacement motor 2-6, liquid feeding bar 2-10, perpendicular displacement screw mandrel 2-12 and ST 2-14, and support 2-5 is threaded with transversal displacement screw mandrel 2-11; The top of support 2-5 is set with perpendicular displacement motor 2-6, the bottom is provided with ST seat 2-13, gets pipe motor 2-16 and is fixedly installed on the ST seat 2-13; Perpendicular displacement screw mandrel 2-12, liquid feeding bar 2-10 and perpendicular displacement slide bar 2-9 laterally arrange; The end of liquid feeding bar 2-10 is fixedly connected with slide block 2-7; The other end of liquid feeding bar 2-10 is provided with ST 2-14 and photoelectric sensor 2-15, and liquid feeding bar 2-10 passes ST seat 2-13 and is provided with.
Perpendicular displacement screw mandrel 2-12 and perpendicular displacement slide bar 2-9 all pass slide block 2-7 and are provided with; The lower end of perpendicular displacement slide bar 2-9 is fixedly connected with ST seat 2-13; Perpendicular displacement slide bar 2-9 and slide block 2-7 are slidingly matched; Perpendicular displacement screw mandrel 2-12 is threaded with slide block 2-7, and perpendicular displacement slide bar 2-9 is provided with register pin; The clutch end of the end of perpendicular displacement screw mandrel 2-12 and perpendicular displacement motor 2-6 is provided with the perpendicular displacement belt wheel, and two perpendicular displacement belt wheels connect through belt.
The clutch end of getting pipe motor 2-16 is provided with the sampling belt wheel, and the sampling belt wheel is connected through belt with ST 2-14; ST 2-14 is a tubular, and inwall is attacked the screw thread with the screw thread 1-3 coupling of the tube wall of antibody pipe.
Transversal displacement screw mandrel 2-11 is vertical with length travel screw mandrel 2-17, transversal displacement screw mandrel 2-11 is all vertical with perpendicular displacement screw mandrel 2-12 with length travel screw mandrel 2-17.
The back program control system instruction perpendicular displacement motor 2-6 that starts working works, and drives slide block 2-7, liquid feeding bar 2-10 and ST seat 2-13 through synchronous pulley and perpendicular displacement screw mandrel 2-12 and moves down.When photoelectric sensor 2-15 senses the piston of antibody pipe, perpendicular displacement motor 2-6 quits work, and starts to get pipe motor 2-16, gets pipe motor 2-16 and drives ST 2-14 rotation, and ST 2-14 and antibody pipe are promptly through being threaded together.Get pipe motor 2-16 then and stop, restarting perpendicular displacement motor 2-6 backward rotation, the antibody pipe is lifted to specified altitude assignment after, perpendicular displacement motor 2-6 stops, and pins the register pin on the perpendicular displacement slide bar 2-9 simultaneously.Through transversal displacement device and length travel device the antibody pipe is moved on the target application of sample position; Start perpendicular displacement motor 2-6; Liquid feeding bar 2-10 moves downward; This moment, ST seat 2-13 went up through perpendicular displacement slide bar 2-9 and register pin locks, through liquid feeding bar 2-10 with outside the antibody blow-off pipe in the antibody pipe.
As shown in Figure 4, temperature control heating arrangement 3 comprises frame 3-5, motor 3-6, portable plate 3-9, section frame 3-1, tank 3-3, semiconductor heating plate 3-12.
The clutch end of motor 3-6 is connected with the power wheel 3-4 that is arranged on the frame 3-5.
Portable plate 3-9 is arranged on frame 3-5 top, and portable plate 3-9 symmetrical, parallel is set with two rhizoid bar 3-8, and two rhizoid bar 3-8 pass frame 3-5 respectively and are provided with, and respectively be arranged on frame 3-5 on two synchronous pulley 3-7 be threaded; Two synchronous pulley 3-7 are connected through belt with power wheel 3-4, realize rotating synchronously.
Section frame 3-1 is connected through cramp bar 3-10 with portable plate 3-9, and section frame 3-1 is provided with row's draw-in groove, and section 3-2 inserts in the draw-in groove; Tank 3-3 is arranged between section frame 3-1 and the portable plate 3-9; Heating frame 3-11 is arranged on the tank 3-3; Semiconductor heating plate 3-12 is arranged on the heating frame 3-11; Every chip semiconductor heating plate 3-12 is corresponding with every section 3-2 position on the section frame, and every chip semiconductor heating plate 3-12 is provided with temperature control sensor 3-13.
When section 3-2 need heat, starter motor 3-6 moved down through power wheel 3-4, synchronous pulley 3-7 and screw mandrel 3-8 drive portable plate 3-9 and section frame 3-1, and when section 3-2 contacted with semiconductor heating plate 3-12, motor 3-6 stopped.Start semiconductor heating plate 3-12 and heat, temperature control sensor 3-13 carries out adjustment control voluntarily.After heating finishes, starter motor 3-6 backwards rotation, section frame 3-1 resets.
In the work, control antibody apparatus for placing 1, get pipe liquid-adding device 2 and temperature control heating arrangement 3 cooperatings, can realize that the immunohistochemical staining experiment automatically carries out, and need not carry out manual operations.
Claims (1)
1. full-automatic immunohistochemical staining appearance comprises base, and base is provided with the antibody apparatus for placing, gets pipe liquid-adding device and temperature control heating arrangement, it is characterized in that:
Described antibody apparatus for placing comprises antibody pipe and pipe support;
The antibody pipe comprises body, and the lower end of body is connected with the liquid feeding head, and the liquid feeding headgear has headgear; Be provided with piston in the body, piston and pull bar are through being threaded, and the top of the sidewall of body has screw thread, and the sidewall of body is provided with spacer pin and scale mark;
Pipe support comprises platform and the platform support vertical with platform, has a plurality of antibody pipe sockets on the platform, and a plurality of antibody pipe sockets are arranged; The edge of each antibody pipe socket has stopper slot, and each antibody pipe socket limit has the headgear socket, and a plurality of headgear sockets are arranged; The stopper slot of pipe support is corresponding with the spacer pin shape, after the antibody pipe inserts antibody pipe socket, guarantees that body can not rotate;
The described pipe liquid-adding device of getting comprises transversal displacement device, length travel device and vertical displacement devices;
The transversal displacement device comprises the transversal displacement motor that is provided with on mobile base and the mobile base; Transversal displacement screw mandrel and transversal displacement slide bar laterally arrange, and transversal displacement screw mandrel and transversal displacement motor are connected;
The length travel device comprises length travel screw mandrel and the length travel slide bar that laterally arranges, and an end and the length travel motor of length travel screw mandrel are connected, and the other end is threaded with the mobile base of transversal displacement device;
Vertical displacement devices comprises support, gets pipe motor, perpendicular displacement motor, liquid feeding bar, perpendicular displacement screw mandrel and ST, and support is connected with the transversal displacement wire rod thread; The top of support is set with the perpendicular displacement motor, the bottom is provided with the ST seat, gets the pipe motor and is fixedly installed on the ST seat; Perpendicular displacement screw mandrel, liquid feeding bar and perpendicular displacement slide bar laterally arrange, and an end of liquid feeding bar is fixedly connected with slide block, and the other end of liquid feeding bar is provided with ST and photoelectric sensor, and the liquid feeding bar passes the setting of ST seat;
Described perpendicular displacement screw mandrel and perpendicular displacement slide bar all pass the slide block setting; The lower end of perpendicular displacement slide bar is fixedly connected with the ST seat; Perpendicular displacement slide bar and slide block are slidingly matched, and the perpendicular displacement screw mandrel is threaded with slide block, and the perpendicular displacement slide bar is provided with register pin; One end of perpendicular displacement screw mandrel and the clutch end of perpendicular displacement motor are provided with the perpendicular displacement belt wheel, and two perpendicular displacement belt wheels connect through belt; The clutch end of getting the pipe motor is provided with the sampling belt wheel, and the sampling belt wheel is connected through belt with ST; Described ST is a tubular, and inwall is attacked the screw thread with the sidewall coupling of the body of antibody pipe;
Described transversal displacement screw mandrel is vertical with the length travel screw mandrel, the transversal displacement screw mandrel is all vertical with the perpendicular displacement screw mandrel with the length travel screw mandrel;
Described temperature control heating arrangement comprises frame, motor, portable plate, section frame, tank, semiconductor heating plate; The clutch end of motor is connected with the power wheel that is arranged on the frame;
Portable plate is arranged on frame top, and the portable plate symmetrical, parallel is set with two rhizoid bars, and two rhizoid bars pass the frame setting respectively, and respectively be arranged on frame on two synchronous pulleys be threaded; Two synchronous pulleys are connected through belt with power wheel, realize rotating synchronously;
The section frame is connected through cramp bar with portable plate, and section is set up and is equipped with row's draw-in groove, and section is inserted in the draw-in groove; Tank is arranged between section frame and the portable plate, and heating frame is arranged on the tank, and the semiconductor heating plate is arranged on the heating frame, and every chip semiconductor heating plate is corresponding with every slice position on the frame of cutting into slices, and every chip semiconductor heating plate is provided with temperature control sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100334494A CN102116712B (en) | 2011-01-31 | 2011-01-31 | Fully-automatic immune tissue chemical dyeing instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100334494A CN102116712B (en) | 2011-01-31 | 2011-01-31 | Fully-automatic immune tissue chemical dyeing instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102116712A CN102116712A (en) | 2011-07-06 |
| CN102116712B true CN102116712B (en) | 2012-07-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100334494A Expired - Fee Related CN102116712B (en) | 2011-01-31 | 2011-01-31 | Fully-automatic immune tissue chemical dyeing instrument |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103364250B (en) * | 2012-04-10 | 2015-12-09 | 深圳迈瑞生物医疗电子股份有限公司 | Push jack overflow dyeing machine color pond stops and tripping device and push jack overflow dyeing machine |
| CN103196732B (en) * | 2013-03-29 | 2015-10-14 | 麦克奥迪（厦门）医疗诊断系统有限公司 | A kind of pathology automated DNA coloring system and colouring method |
| CN105675383B (en) * | 2016-03-21 | 2019-06-14 | 厦门通灵生物医药科技有限公司 | A kind of multichannel slide glass sample incubating device with heating function |
| CN107202879B (en) * | 2017-05-18 | 2018-12-25 | 杭州依美洛克医学科技有限公司 | A kind of biochemical test sample adding system |
| CN109211619B (en) * | 2017-06-29 | 2021-01-05 | 宁夏六盘机器人系统工程有限公司 | Lithium battery electrolyte quantitative sampling equipment |
| CN113515077A (en) * | 2021-04-23 | 2021-10-19 | 重庆德方信息技术有限公司 | System and method for monitoring human body cell staining process |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2066578U (en) * | 1990-05-29 | 1990-11-28 | 华中农业大学 | Automatic dehydrating dyeing meter for boilogical micro-sample |
| US7521021B2 (en) * | 2003-11-26 | 2009-04-21 | Leica Biosvstems St. Louis Llc | System for in situ processing of a tissue specimen |
| US8758707B2 (en) * | 2008-06-16 | 2014-06-24 | Leica Biosystems Melbourne Pty Ltd | Staining instruments and methods |
| CN201331459Y (en) * | 2009-01-15 | 2009-10-21 | 孟平 | Full-automatic paraffin slice dyeing instrument |
| CN201681010U (en) * | 2010-04-13 | 2010-12-22 | 滕文友 | Automation multi-function staining apparatus |
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Address after: 311215 Zhejiang economic and Technological Development Zone, Xiaoshan District, Xiaoshan, No. four, building No. 1368 Road, No. Patentee after: ZHEJIANG CENTURY CONDOR MEDICAL SCIENCE & TECHNOLOGY Co.,Ltd. Address before: 311215 Construction No. four, No. 99, Xiaoshan District, Zhejiang, Hangzhou Patentee before: ZHEJIANG CENTURY CONDOR MEDICAL SCIENCE & TECHNOLOGY Co.,Ltd. |
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