CN102100714A - Method for extracting active ingredients of Echinacea through supercritical carbon dioxide - Google Patents
Method for extracting active ingredients of Echinacea through supercritical carbon dioxide Download PDFInfo
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- CN102100714A CN102100714A CN 200910255655 CN200910255655A CN102100714A CN 102100714 A CN102100714 A CN 102100714A CN 200910255655 CN200910255655 CN 200910255655 CN 200910255655 A CN200910255655 A CN 200910255655A CN 102100714 A CN102100714 A CN 102100714A
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- echinacea
- carbon dioxide
- supercritical carbon
- dioxide extraction
- effective ingredient
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Abstract
The invention relates to a method for extracting active ingredients of Echinacea through supercritical carbon dioxide. The method mainly comprises the processes of: drying whole Echinacea; performing ultramicro crushing, performing supercritical carbon dioxide extraction, performing primary separation under reduced pressure, performing secondary separation under reduced pressure, and obtaining extract liquor of active ingredients of Echinacea. The method has the characteristics that: the extraction temperature is low, the speed is high, the extraction rate is high, solvent residue is avoided, the obtained active ingredients are high in content and have no undesirable influence on human and environment, and the like.
Description
Technical field
The present invention relates to a kind of extracting process of effective ingredients in plant, particularly utilize the method for a kind of Echinacea effective ingredient of supercritical carbon dioxide extraction, belong to medical technical field.
Background technology
Echinacea (Echinacea) is a kind of Echinacea plant that originates in North America and Canada south, this platymiscium has 8 kinds and several mutation, present 3 kinds that mainly contain: Echinacea purpurea Moench (Echinacea purpurea as drug development, be usually said Echinacea), Echinacea angustifolia (Echinacea augustifolia) and white Echinacea (Echinacen pallida).At present, wild amount seldom, they originate in the North America, now Europe and other are regional all an introducing and planting.Ground such as China Beijing, Nanjing, Shanghai and Changsha are also introduced a fine variety and are carried out the GAP kind and plant.The main chemical compositions that Echinacea, Echinacea angustifolia and violet ash awl chrysanthemum contain can be divided into several big classes: polysaccharide and glycoprotein, polar caffeic acid derivant (caffeic acid derivatives), fat-soluble alkylamide (alkamides).Every constituents that different plants contain has difference, and the contained chemical compound of every constituents also is not quite similar.For example, the polarity part that leaches at ethanol mainly contains the caffeic acid derivant, Echinacea angustifolia and violet ash awl chrysanthemum mainly contain Echinacea glycoside (echinacoside), and Echinacea then mainly contains chicoric acid (cichoric acid), can be used as immunomodulator and stimulant.
China has abundant animal and plant resource, and the application of Chinese herbal medicine also has history in several thousand.The utilization modern science and technology can promote human beings'health to the research (comprising separation, purification, structure determination, structure and function etc.) of natural product active ingredient, control to livestock and poultry also can be more safe and effective, and it has become the trend of new century natural product research.And promptly be wherein a kind of for the research of Echinacea.Echinacea has long use history in America, U.S. aborigines once were used as Echinacea " cure-all medicine ", is used for treating back of the body saddle skin ulcer, insecticide and venom, blood viral disease disease of infection and wound, flu, headache, horse etc.At present Echinacea in America as immunopotentiating agent and immunomodulator, be used for the treatment of virosis or bacterial acute or various serious bacterial infections of the various antibiotics of chronic respiratory tract infection auxiliary treatment etc., what this wherein really brought into play drug effect is effective ingredient such as polysaccharide, chicoric acid and flavone.Present extraction for Echinacea, be limited to traditional reflux method more, the method that the ultrasonic extraction used is also arranged, but all there are some drawbacks in these two kinds of methods, the temperature that heating reflux method needs is higher, time is longer, and therefore the part effective ingredient can lose activity and can not be extracted fully; Though supercritical ultrasonics technology can shorten extraction time, also not high to the requirement of temperature, the strong cavitation effect that ultrasound wave produced can cause the part loss of active ingredients equally.
Summary of the invention
Supercritical carbon dioxide extraction method of the present invention has overcome the deficiency of existing extractive technique, and the method for the supercritical carbon dioxide extraction Echinacea effective ingredient that a kind of extraction temperature is low, speed is fast, extraction yield is high, the gained active constituent content is high is provided.
To achieve these goals, the technical solution used in the present invention is: Echinacea herb oven dry → micronizing → supercritical carbon dioxide extraction → one-level decompression separation → second depressurized separation → Echinacea effective ingredient extract.
Advantage of the present invention is:
1. extraction temperature is low, speed is fast, extraction yield is high
2. no solvent residue
3. human and environment is had no adverse effects
The specific embodiment
Below embodiments of the invention are described in further detail:
The present invention is a kind of method of supercritical carbon dioxide extraction Echinacea effective ingredient, it is characterized in that: Echinacea herb oven dry → micronizing → supercritical carbon dioxide extraction → one-level decompression separation → second depressurized separation → Echinacea extracts active ingredients liquid.
Described Echinacea herb bake out temperature is 50～80 ℃, and drying time is 4～6 hours.
Described Echinacea herb oven dry back grinding particle size is 60～80 orders.
In the described extractor with Echinacea herb powder input supercritical carbon dioxide extraction apparatus, with supercritical carbon dioxide as solvent, 60～80% ethanol are done entrainer, at pressure is 10～20MPa, temperature is to extract under 40～60 ℃ of conditions, and the supercritical carbon dioxide extraction time is 1～2 hour.
The described one-level decompression separation pressure that carries out in knockout drum is 5～10MPa, and temperature is 40～60 ℃; Second depressurized separating pressure position 5～7MPa, temperature is 30～50 ℃.
Embodiment
Get the Echinacea medical material 100g of micronizing, grinding particle size is 80 orders, and drop in the extraction kettle oven dry back, and extraction conditions is: extracting pressure is 15MPa, and temperature is 45 ℃; Separation condition is: flash trapping stage still pressure is 6MPa, and temperature is 45 ℃, and secondary separating still pressure is 5MPa, and temperature is 35 ℃; 80% ethanol is done entrainer.Behind the dynamic operation 2 hours, obtain the extract of Echinacea effective ingredient from flash trapping stage still and secondary separating still.
Claims (5)
1. the method for a supercritical carbon dioxide extraction Echinacea effective ingredient is characterized in that, Echinacea herb oven dry → micronizing → supercritical carbon dioxide extraction → one-level decompression separation → second depressurized separation → Echinacea effective ingredient extract.
2. the method for supercritical carbon dioxide extraction Echinacea effective ingredient according to claim 1 is characterized in that: Echinacea herb bake out temperature is 50～80 ℃, and drying time is 4～6 hours.
3. the method for supercritical carbon dioxide extraction Echinacea effective ingredient according to claim 1 is characterized in that: Echinacea herb oven dry back grinding particle size is 60～80 orders.
4. the method for supercritical carbon dioxide extraction Echinacea effective ingredient according to claim 1, it is characterized in that: Echinacea herb powder is dropped in the extractor of supercritical carbon dioxide extraction apparatus, with supercritical carbon dioxide as solvent, 60～80% ethanol are done entrainer, at pressure is 10～20MPa, temperature is to extract under 40～60 ℃ of conditions, and the supercritical carbon dioxide extraction time is 1～2 hour.
5. the method for supercritical carbon dioxide extraction Echinacea effective ingredient according to claim 1 is characterized in that: carrying out one-level decompression separation pressure in knockout drum is 5～10MPa, and temperature is 40～60 ℃; Second depressurized separating pressure position 5～7MPa, temperature is 30～50 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910255655 CN102100714A (en) | 2009-12-16 | 2009-12-16 | Method for extracting active ingredients of Echinacea through supercritical carbon dioxide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910255655 CN102100714A (en) | 2009-12-16 | 2009-12-16 | Method for extracting active ingredients of Echinacea through supercritical carbon dioxide |
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| CN102100714A true CN102100714A (en) | 2011-06-22 |
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| CN 200910255655 Pending CN102100714A (en) | 2009-12-16 | 2009-12-16 | Method for extracting active ingredients of Echinacea through supercritical carbon dioxide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498192A (en) * | 2014-12-15 | 2015-04-08 | 云南中烟工业有限责任公司 | Preparation method and application of florists chrysanthemum extract |
| EP3034129A1 (en) * | 2014-12-16 | 2016-06-22 | Agon Pharma GmbH | Method for producing an echinacea purpura dry press juice and use of same for the treatment of equinem sarcoid |
-
2009
- 2009-12-16 CN CN 200910255655 patent/CN102100714A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498192A (en) * | 2014-12-15 | 2015-04-08 | 云南中烟工业有限责任公司 | Preparation method and application of florists chrysanthemum extract |
| EP3034129A1 (en) * | 2014-12-16 | 2016-06-22 | Agon Pharma GmbH | Method for producing an echinacea purpura dry press juice and use of same for the treatment of equinem sarcoid |
| WO2016096972A1 (en) * | 2014-12-16 | 2016-06-23 | Agon Pharma Gmbh | Method for producing an echinacea purpurea dry pressed juice, and the use of same for treating equine sarcoids |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Application publication date: 20110622 |