CN102093973B - Bacterial strain Pandoraea sp. B-6 for processing high-density papermaking black liquor and application - Google Patents
Bacterial strain Pandoraea sp. B-6 for processing high-density papermaking black liquor and application Download PDFInfo
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- CN102093973B CN102093973B CN201010578692A CN201010578692A CN102093973B CN 102093973 B CN102093973 B CN 102093973B CN 201010578692 A CN201010578692 A CN 201010578692A CN 201010578692 A CN201010578692 A CN 201010578692A CN 102093973 B CN102093973 B CN 102093973B
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Abstract
The invention discloses a bacterial strain Pandoraea sp. B-6 for processing high-density papermaking black liquor, which has the collection number of CGMCC (China General Microbiological Culture Collection) No.4239. The inventor collects Wu kingdom bamboo slip soak solution with plaque in the three kingdoms period, which is collected in the Changsha Bamboo Slips Museum, and uses the Wu kingdom bamboo slip soak solution as raw material, and one bacterial strain capable of growing on a culture medium which takes the alkali lignin as the only carbon source is obtained by accumulation culture, isolation, purification and screening. The bacteria strain can be directly used for processing high-density alkaline straw pulp papermaking black liquor which is not subjected to materialization preprocessing, and the COD (Chemical Oxygen Demand) of 30,000mg/L of the high-density alkaline straw pulp papermaking black liquor with the initial pH value of 11 and chroma of 8,500 can be lowered by 39.1% within five days.
Description
Technical field
The present invention relates to the biologic treating technique of high density black liquid, particularly a strain is used for the bacterium and the application thereof of the alkaline straw pulp paper black liquid of direct treatment of high concentration, high basicity, high chroma.
Background technology
Paper-making industrial waste water accounts for 10% of the total wastewater flow rate of whole nation industry, and the pollution that the water resource environment of China is caused is on the rise.The black liquid that produces in the pulping process is the important source of pollution of paper industry.Straw pulp is the main slurry kind of Made in China paper plant, and straw pulp alkaline cooking black liquor is the principal pollutant in the domestic paper mill of papermaking.Silvola recovery is the most sophisticated treating paper-making black liquor of present stage; But and be not suitable for the improvement of straw pulp black liquor; And investment is big, running cost is high; Cause most of papermaking enterprises black digestion liquid not to be administered or not exclusively administered and discharge, caused the serious environmental pollution, become the significant problem of restriction paper industry development.
Treatment process for black liquid mainly also has at present: Silvola recovery, acid-precipitation method, alkali are analysed method, membrane separation process, flocculent precipitation, their each tool relative merits, but all can not reach good effect, limited the application in administration of papermaking black liquid.Biological treatment is the organism that utilizes in the microbiological oxidation a large amount of existence of nature, that rely on the organism life, the decomposition black liquor; Take certain artificial measures simultaneously; Creation helps the environment of microorganism growth, breeding, improves the efficient of microbiological oxidation, decomposing organic matter.In recent years, domestic the straw pulp black liquor biologic treating technique has been carried out many researchs, also obtained certain achievement, some technology is through pilot scale.But these traditional biological treatment process are applied to some defectives below the black liquor treatment ubiquity: 1, black liquor pH is high, could get into biological processing unit after needing to regulate pH earlier; 2, in the black liquor salt ion of some high densitys to toxic effect of bacterium such as Na
+3, contain tannin etc. in the black liquor to the virose material of mikrobe, the traditional biological method can only be handled the black liquor after dilution or the detoxification; 4, alkaline cooking dissolved xylogen is difficult to biological degradation, and most of microbe can not be degraded and utilized xylogen; 5, the black liquor biodegradability is poor, and it is poor that mud increases.Conventional thus biotechnology treatment process can not be carried out black liquor treatment well.
Summary of the invention
The purpose of this invention is to provide bacterial strain Pandoraea sp.B-6 and application that a strain is used for the treatment of high concentration black liquid.
One strain is used for the bacterial strain Pandoraea sp.B-6 of treatment of high concentration black liquid, and the deposit number of said bacterial strain is CGMCC No.4239.
The substratum of described bacterial strain consists of: alkali lignin 3g, (NH
4)
2SO
42g, K
2HPO
41g, MgSO
40.2g, CaCl
20.1g, FeSO
40.05, MnSO
40.02g, KH
2PO
41g, agar 15g, zero(ppm) water 1000mL, pH are 7.0～7.4.
Said bacterial strain directly is used for alkaline straw pulp paper black liquid to be handled.
The present invention through gathering, separate, taming, obtains the bacterium that a strain can directly be used to handle high COD, high chroma and high pH black liquid from black liquid microbiological treatment technical standpoint.
In conjunction with the bacterium colony morphological features with based on the Phylogenetic Analysis of bacterial 16 S rDNA gene order, be the Pandoraea bacterium with this dientification of bacteria, called after Pandoraea sp.B-6.This bacterial strain is submitted biological preservation on October 22nd, 2010 to China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), and preserving number is CGMCC No.4239.This bacterium can directly be used to handle the high density alkaline process black liquid of straw pulp paper-making without any physico-chemical pretreatment, can effectively reduce COD, colourity and the pH of black liquor.
The contriver is according to a collection of unearthed the period of Three Kingdoms Wu state bamboo slip used for writing on during ancient times in building of galloping along on horseback of Changsha letter wooden tablets or slips for writing museum collection, and in the back preservation process of being unearthed, it is sick to have broken out the bamboo slip used for writing on during ancient times plaque; Bamboo slip used for writing on during ancient times bamboo body is by microbial attack; Xylogen wherein, Mierocrystalline cellulose are degraded by certain or certain group of Institute of Micro-biology, and the bamboo slip used for writing on during ancient times bamboo body in the plaque becomes translucent membranaceous material, and the situation of breaking easily and coming off; From the soak solution of this batch bamboo slip used for writing on during ancient times, separate, filter out bacterial strain of the present invention.
The separation of bacterial strain of the present invention, purifying and screening process are:
The bamboo slip used for writing on during ancient times soak solution of letter wooden tablets or slips for writing museum, Changsha sealing preservation is inoculated rich medium respectively carry out enrichment culture.Culture combines the line partition method on corresponding solid plate, to separate with dilution-plate method, until the pure culture that obtains various mikrobes.Culture temperature is 30 ℃.It is on the solid plate screening culture medium of sole carbon source that the bacterial strain pure culture that separation is obtained is inoculated into the xylogen; Put into biochemical incubator and leave standstill cultivation at 30 ℃ of following constant temperature; Observe every day, and according to the growing state of bacterial strain on the flat board, the line separation obtains final pure culture bacterial strain again.
Said rich medium consists of: Tryptones 10g, and yeast extract 5g, sodium-chlor 5g, agar 15-20g, zero(ppm) water 1000mL, the pH value is 7.0～7.4.Said xylogen is that the substratum of sole carbon source consists of: alkali lignin 3g, (NH
4)
2SO
42g, K
2HPO
41g, MgSO
40.2g, CaCl
20.1g, FeSO
40.05, MnSO
40.02g, KH
2PO
41g, agar 15g, zero(ppm) water 1000mL, pH are 7.0～7.4.
The present invention has remedied the deficiency that conventional mikrobe can not directly be used for the treatment of high concentration black liquid.Bacterial strain Pandoraea sp.B-6 according to the invention can directly drop into pH=11, and COD is up to 30000mg/L, and colourity is to handle in 8500 the high density black liquid, through 5 days biological treatment, can make the COD of black liquor descend 39.1%.So far, the bacterial strain that does not belong to relevant for Pandoraea as yet both at home and abroad is applied to the report that black liquid is handled, and the present invention provides a kind of new Microbial resources for the biologic treating technique of black liquid.
Description of drawings
Fig. 1: the colonial morphology figure of bacterial strain of the present invention;
Fig. 2: the thalline sem photograph of bacterial strain of the present invention;
Fig. 3: the COD clearance of bacterial strain treatment of high concentration black liquid of the present invention and thalli growth situation.
Embodiment:
Be intended to further specify the present invention below in conjunction with embodiment, and unrestricted the present invention.
Embodiment 1: the biological assay of bacterial strain
1. strain morphology characteristic
(1) colony morphology characteristic: bacterium colony is light green, and the surface is flat and smooth, the edge is wavy, sees Fig. 1.
(2) morphological features: bacterial strain is carried out gramstaining, utilize opticmicroscope observation, show that this bacterial strain is the rod-short Gram-negative bacteria; And utilizing the sem observation bacterial strain, the bacterial strain stereoscan photograph is seen Fig. 2.
2. bacterial strain 16S rDNA analyzes
Adopt bacterial genomes DNA extraction test kit; The extracting strain gene group DNA is a template with the strain gene group DNA, and primer is bacterial 16 S rDNA universal primer (27F; 1492R); The 16SrDNA fragment of amplification bacterial strain is carried out the Blast homology with the nucleic acid database among the bacterial 16 S rDNA sequence that obtains and the NCBI and is compared (www.ncbi.nlm.nih.gov/Blast), has reached 100% with the similarity of the 16S rDNA sequence of known bacterium Pandoraea sp.JBl in the DB; With this identification of strains is that Pandoraea belongs to called after Pandoraea sp.B-6.
Embodiment 2: bacterial strain is to the treatment effect of high density black liquid
With rich medium (Tryptones 10g, yeast extract 5g, sodium-chlor 5g, the zero(ppm) water 1000mL of bacterium at liquid; The pH value is 7.0～7.4) in be cultured to logarithmic phase, be inoculated into initial pH=11 by the inoculum size of 1% (V/V), COD is 30000mg/L, colourity is in 8500 the high density alkaline process black liquid of straw pulp paper-making; At 30 ℃, shaking culture is 5 days under the 120r/mm, and take a sample once every day, and sample is through the centrifugal 6min of 12000r/min; Deposition is with after the sterilized water washed twice, and repeated centrifugation is operated, 80 ℃ of oven dry; Weigh, obtain dry cell weight, be used to measure the strain growth situation; Measure supernatant C OD, be used to detect the COD clearance of bacterial strain to black liquid, the result sees Fig. 3.
Claims (1)
1. a strain is used for the application method of the bacterial strain Pandoraea sp.B-6 of treatment of high concentration black liquid, it is characterized in that, said bacterial strain direct inoculation is handled in alkaline straw pulp paper black liquid, and the deposit number of said bacterial strain is CGMCC No.4239.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010578692A CN102093973B (en) | 2010-12-08 | 2010-12-08 | Bacterial strain Pandoraea sp. B-6 for processing high-density papermaking black liquor and application |
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|---|---|---|---|
| CN201010578692A CN102093973B (en) | 2010-12-08 | 2010-12-08 | Bacterial strain Pandoraea sp. B-6 for processing high-density papermaking black liquor and application |
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| CN102093973A CN102093973A (en) | 2011-06-15 |
| CN102093973B true CN102093973B (en) | 2012-09-05 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559542B (en) * | 2011-12-14 | 2013-06-05 | 首创爱华(天津)市政环境工程有限公司 | Strain Pandoraea sp. for removing ammonia nitrogen in sewage under low-temperature condition and isolated culture method |
| CN102633405B (en) * | 2012-04-01 | 2013-10-09 | 中南大学 | Treatment method of papermaking black liquor |
| CN102978142B (en) * | 2012-12-13 | 2014-02-05 | 湖南大学 | Rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin |
| CN103756928B (en) * | 2013-11-26 | 2015-09-30 | 浙江大学 | For the bacterial strain of p-Xylol of degrading and cultural method thereof and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1182149A (en) * | 1996-10-09 | 1998-05-20 | 王允洪 | Disposal method of papermaking black liquor |
| CN1305783C (en) * | 2004-02-27 | 2007-03-21 | 华中科技大学 | Biological treatment method for paper making black liquor |
-
2010
- 2010-12-08 CN CN201010578692A patent/CN102093973B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1182149A (en) * | 1996-10-09 | 1998-05-20 | 王允洪 | Disposal method of papermaking black liquor |
| CN1305783C (en) * | 2004-02-27 | 2007-03-21 | 华中科技大学 | Biological treatment method for paper making black liquor |
Non-Patent Citations (2)
| Title |
|---|
| 柴立元等.三国吴简蚀斑可培养微生物的多样性.《中南大学学报(自然科学版)》.2010,第41卷(第5期),1674-1679. * |
| 陈跃辉.三国吴简腐蚀斑微生物的分离鉴定及其木质素降解性能研究.《中国优秀硕士学位论文全文数据库工程科技I辑》.2010,(第4期),B027-97. * |
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