CN102093972A - Bacterial strain Comamonas sp. B-9 for processing high-density papermaking black liquor and application thereof - Google Patents
Bacterial strain Comamonas sp. B-9 for processing high-density papermaking black liquor and application thereof Download PDFInfo
- Publication number
- CN102093972A CN102093972A CN 201010578660 CN201010578660A CN102093972A CN 102093972 A CN102093972 A CN 102093972A CN 201010578660 CN201010578660 CN 201010578660 CN 201010578660 A CN201010578660 A CN 201010578660A CN 102093972 A CN102093972 A CN 102093972A
- Authority
- CN
- China
- Prior art keywords
- bacterial strain
- black liquor
- papermaking black
- comamonas
- density
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention discloses a bacterial strain Comamonas sp. B-9 for processing high-density papermaking black liquor, which has the collection number of CGMCC (China General Microbiological Culture Collection) No.4251. The inventor collects Wu kingdom bamboo slip soak solution with plaque in the three kingdoms period, which is collected in the Changsha Bamboo Slips Museum, and uses the Wu kingdom bamboo slip soak solution as raw material, and one bacterial strain capable of growing on a culture medium which takes the alkali lignin as the only carbon source is obtained by accumulation culture, isolation, purification and screening. The bacteria strain can be directly used for processing high-density alkaline straw pulp papermaking black liquor which is not subjected to any materialization preprocessing, and the COD (Chemical Oxygen Demand) of 30,000mg/L of the high-density alkaline straw pulp papermaking black liquor with the initial pH value of 11and chroma of 8,500 can be lowered by 38.9% within five days.
Description
Technical field
The present invention relates to the biologic treating technique of high density black liquid, particularly a strain is used for directly handling the bacterium and the application thereof of the alkaline straw pulp paper black liquid of high density, high basicity, high chroma.
Background technology
Paper-making industrial waste water accounts for 10% of the total wastewater flow rate of whole nation industry, and the pollution that the water resource environment of China is caused is on the rise.The black liquid that produces in the pulping process is the important source of pollution of paper industry.Straw pulp is the main slurry kind of Made in China paper plant, and straw pulp alkaline cooking black liquor is the principal pollutant in the domestic paper mill of papermaking.Silvola recovery is the most sophisticated treating paper-making black liquor of present stage, but and be not suitable for the improvement of straw pulp black liquor, and investment is big, running cost is high, cause most of papermaking enterprises black digestion liquid not to be administered or not exclusively administered discharging, cause the serious environmental pollution, become the significant problem of restriction paper industry development.
Treatment process for black liquid mainly also has at present: acid-precipitation method, alkali are analysed method, membrane separation process, flocculent precipitation, their each tool relative merits, but all can not reach good effect, limited the application in administration of papermaking black liquid.Biological treatment is the organism that utilizes in the microbiological oxidation a large amount of existence of nature, that rely on the organism life, the decomposition black liquor, take certain artificial measures simultaneously, creation helps the environment of microorganism growth, breeding, improves the efficient of microbiological oxidation, decomposing organic matter.In recent years, domestic the straw pulp black liquor biologic treating technique has been carried out many researchs, also obtained certain achievement, some technology is by pilot scale.But these traditional biological treatment process are applied to the following defectives of black liquor treatment ubiquity: 1, black liquor pH height just can enter biological processing unit after needing to regulate pH earlier; 2, in the black liquor salt ion of some high densitys to toxic effect of bacterium such as Na
+3, contain tannin etc. in the black liquor to the virose material of microorganism, the traditional biological method can only be handled the black liquor after dilution or the detoxification; 4, alkaline cooking dissolved xylogen is difficult to biological degradation, and most of microbe can not be degraded and be utilized xylogen; 5, the black liquor biodegradability is poor, and it is poor that mud increases.Chang Gui biotechnology treatment process can not be carried out black liquor treatment well thus.
Summary of the invention
The purpose of this invention is to provide bacterial strain Comamonas sp.B-9 and application that a strain is used to handle the high density black liquid.
The deposit number that one strain is used to handle the bacterial strain Comamonas sp.B-9 of high density black liquid is CGMCC No.4251.
The substratum of described bacterial strain consists of: alkali lignin 3g, (NH
4)
2SO
42g, K
2HPO
41g, MgSO
40.2g, CaCl
20.1g, FeSO
40.05, MnSO
40.02g, KH
2PO
41g, agar 15g, distilled water 1000mL, pH are 7.0～7.4.
Described bacterial strain is directly used in alkaline straw pulp paper black liquid and handles.
The present invention, obtains a strain and can be directly used in the bacterium of handling high COD, high chroma and high pH black liquid by gathering, separate, taming from black liquid microbiological treatment technical standpoint.
In conjunction with the bacterium colony morphological features with based on the Phylogenetic Analysis of bacterial 16 S rDNA gene order, be the Comamonas bacterium with this dientification of bacteria, called after Comamonas sp.B-9.This bacterial strain is submitted biological preservation on October 22nd, 2010 to China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), and preserving number is CGMCC No.4251.This bacterium can be directly used in the high density alkaline process black liquid of straw pulp paper-making of processing without any physico-chemical pretreatment, can effectively reduce COD, colourity and the pH of black liquor.
The contriver is according to a collection of unearthed the period of Three Kingdoms Wu state bamboo slip used for writing on during ancient times in building of galloping along on horseback of Changsha letter wooden tablets or slips for writing museum collection, in the back preservation process of being unearthed, broken out bamboo slip used for writing on during ancient times plaque disease, bamboo slip used for writing on during ancient times bamboo body is by microbial attack, xylogen wherein, Mierocrystalline cellulose are degraded by certain or certain group of Institute of Micro-biology, and the bamboo slip used for writing on during ancient times bamboo body in the plaque becomes translucent membranaceous material, and the situation of breaking easily and coming off, from the soak solution of this batch bamboo slip used for writing on during ancient times, separate, filter out bacterial strain of the present invention.
The separation of bacterial strain of the present invention, purifying and screening process are:
The bamboo slip used for writing on during ancient times soak solution of letter wooden tablets or slips for writing museum, Changsha sealing preservation is inoculated rich medium respectively carry out enrichment culture.Culture separates on corresponding solid plate in conjunction with the line partition method with dilution-plate method, until the pure culture that obtains various microorganisms.Culture temperature is 30 ℃.It is on the solid plate screening culture medium of sole carbon source that the bacterial strain pure culture that separation is obtained is inoculated into the xylogen, put into biochemical incubator and leave standstill cultivation at 30 ℃ of following constant temperature, observe every day, and according to the growing state of bacterial strain on the flat board, the line separation obtains final pure culture bacterial strain again.
Described rich medium consists of: Tryptones 10g, and yeast extract 5g, sodium-chlor 5g, agar 15-20g, distilled water 1000mL, the pH value is 7.0～7.4.Described xylogen is that the substratum of sole carbon source consists of: alkali lignin 3g, (NH
4)
2SO
42g, K
2HPO
41g, MgSO
40.2g, CaCl
20.1g, FeSO
40.05, MnSO
40.02g, KH
2PO
41g, agar 15g, distilled water 1000mL, pH are 7.0～7.4.
The present invention has remedied conventional microorganism can not be directly used in the deficiency of handling the high density black liquid.Bacterial strain Comamonas sp.B-9 of the present invention can directly drop into pH=11, and COD is up to 30000mg/L, and colourity is to handle in 8500 the high density black liquid, through 5 days biological treatment, can make the COD of black liquor descend 38.9%.So far, at home and abroad there is no the bacterial strain that belongs to about Comamonas and be applied to the report that black liquid is handled, the present invention provides a kind of new Microbial resources for the biologic treating technique of black liquid.
Description of drawings
Fig. 1: the colonial morphology figure of bacterial strain of the present invention;
Fig. 2: the thalline sem photograph of bacterial strain of the present invention;
Fig. 3: bacterial strain of the present invention is handled the COD clearance and the thalli growth situation of high density black liquid.
Embodiment:
Be intended to further specify the present invention below in conjunction with embodiment, and unrestricted the present invention.
Embodiment 1: the biological assay of bacterial strain
1. strain morphology feature
(1) colony morphology characteristic: light green, surface irregularity, bacterium colony is flat, and the edge spination is seen Fig. 1.
(2) morphological features: bacterial strain is carried out gramstaining, utilize opticmicroscope observation, show that this bacterial strain is the rod-short Gram-negative bacteria; And utilizing the sem observation bacterial strain, the bacterial strain stereoscan photograph is seen Fig. 2.
2. bacterial strain 16S rDNA analyzes
Adopt bacterial genomes DNA extraction test kit, the extracting strain gene group DNA, with the strain gene group DNA is template, primer is bacterial 16 S rDNA universal primer (27F, 1492R), the 16SrDNA fragment of amplification bacterial strain, the bacterial 16 S rDNA sequence and the nucleic acid database among the NCBI that obtain are carried out Blast homology comparison (www.ncbi.nlm.nih.gov/Blast), reached 99% with the similarity of the 16S rDNA sequence of known bacterium Comamonas sp.TK41 in the database, with this identification of strains is that Comamonas belongs to called after Comamonas sp.B-9.
Embodiment 2: bacterial strain is to the treatment effect of high density black liquid
With rich medium (the Tryptones 10g of bacterium at liquid, yeast extract 5g, sodium-chlor 5g, distilled water 1000mL, the pH value is 7.0～7.4) in be cultured to logarithmic phase, be inoculated into initial pH=11 by the inoculum size of 1% (V/V), COD is 30000mg/L, colourity is that at 30 ℃, shaking culture is 5 days under the 120r/mm in 8500 the high density alkaline process black liquid of straw pulp paper-making, sampling every day once, sample is through the centrifugal 6min of 12000r/min, and after precipitation was used the sterilized water washed twice, repeated centrifugation was operated, 80 ℃ of oven dry, weigh, obtain dry cell weight, be used to measure the strain growth situation; Measure supernatant C OD, be used to detect the COD clearance of bacterial strain, the results are shown in Figure 3 black liquid.
Claims (4)
1. a strain is used to handle the bacterial strain Comamonas sp.B-9 of high density black liquid, it is characterized in that the deposit number of described bacterial strain is CGMCC No.4251.
2. a substratum of cultivating the described bacterial strain of claim 1 is characterized in that, consists of: alkali lignin 3g, (NH
4)
2SO
42g, K
2HPO
41g, MgSO
40.2g, CaCl
20.1g, FeSO
40.05, MnSO
40.02g, KH
2PO
41g, agar 15g, distilled water 1000mL, pH are 7.0～7.4.
3. the described bacterial strain of claim 1 is applied to handle black liquid.
4. application according to claim 3 is characterized in that, described bacterial strain direct inoculation is handled in alkaline straw pulp paper black liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010578660 CN102093972A (en) | 2010-12-08 | 2010-12-08 | Bacterial strain Comamonas sp. B-9 for processing high-density papermaking black liquor and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010578660 CN102093972A (en) | 2010-12-08 | 2010-12-08 | Bacterial strain Comamonas sp. B-9 for processing high-density papermaking black liquor and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102093972A true CN102093972A (en) | 2011-06-15 |
Family
ID=44127239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010578660 Pending CN102093972A (en) | 2010-12-08 | 2010-12-08 | Bacterial strain Comamonas sp. B-9 for processing high-density papermaking black liquor and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102093972A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633405A (en) * | 2012-04-01 | 2012-08-15 | 中南大学 | Treatment method of papermaking black liquor |
-
2010
- 2010-12-08 CN CN 201010578660 patent/CN102093972A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 《Journal of chemical technology and biotechnology》 19921231 Mittar D等 Bioleaching of pulp and paper will effluents by phanerochaete chrysosporium 81-92 第51卷, 第1期 2 * |
| 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑 （月刊）》 20100415 陈跃辉 三国吴简腐蚀斑微生物的分离鉴定及其木质素降解性能研究 B027-97 , 第4期 2 * |
| 《工业水处理》 20041130 苏维丰 等 造纸黑液综合治理的研究进展 4-8 第24卷, 第11期 2 * |
| 《环境工程》 20020630 孙先锋 等 造纸黑液木质素降解微生物的分离和降解特性研究 78-80 第20卷, 第3期 2 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633405A (en) * | 2012-04-01 | 2012-08-15 | 中南大学 | Treatment method of papermaking black liquor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105255782B (en) | There is fiber bacterium and the purposes of reducing power to Cr VI | |
| CN109161497B (en) | Microbial preparation for degrading aflatoxin and application | |
| CN102093971A (en) | Bacterial strain Cupriavidus sp. B-8 for processing high-density papermaking black liquor and application thereof | |
| CN103352013A (en) | Lignocellulose degrading complex bacterium system and application | |
| CN108220169B (en) | Separation screening and identification method of strain for degrading polystyrene | |
| CN113174334B (en) | Method for screening simplified flora of sugarcane rhizosphere growth-promoting bacteria | |
| CN102093973B (en) | Bacterial strain Pandoraea sp. B-6 for processing high-density papermaking black liquor and application | |
| CN110343641B (en) | Bacillus salinus SYW-1 and application thereof in prevention and treatment of cotton verticillium wilt | |
| CN102633405B (en) | Treatment method of papermaking black liquor | |
| CN117363498B (en) | Wick ham yeast CYW-7 and application thereof | |
| CN106834165B (en) | Paracoccus capable of degrading penicillin, cell fraction and composition thereof | |
| CN107142230B (en) | Garcinia microsphere strain and application thereof | |
| CN102093972A (en) | Bacterial strain Comamonas sp. B-9 for processing high-density papermaking black liquor and application thereof | |
| CN109337825A (en) | One plant of Beijing Paecilomyces varioti bacterial strain LYZ7 and its application | |
| CN108676732A (en) | Vacation Escherichia WNF15 one plant oval and its application | |
| CN111621438B (en) | Wedner mannich bacillus LM-LZ separated from oxidation pond of pig farm and application thereof | |
| CN104560825B (en) | It is a kind of can the husky streptomycete of high-efficiency lignin degrading and its application in the basic conditions | |
| CN108102942A (en) | One plant of bacterial strain and its application for being used to purify molasses alcohol waste water | |
| CN109628355B (en) | Sulfide degrading bacteria and application thereof | |
| CN107236683B (en) | Slow staphylococcus strain and application thereof | |
| CN111235052A (en) | Thermophilic bacillus licheniformis and application thereof | |
| CN104962495B (en) | A kind of wheat anthropi of degradable lincomycin | |
| CN114891655B (en) | Application of stenotrophomonas SY1 immobilized microbial inoculum in purification of cadmium and chromium pollution | |
| CN103103140A (en) | Aerobic phosphorus removing bacterial stain for sewage treatment and application thereof | |
| CN110819538B (en) | Fusarium aromaticum HNU066 and application thereof in degradation of waste gas containing hydrogen sulfide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110615 |