CN102090391A - Method for enhancing control effect of biological control agent for plant parasitic nematodes - Google Patents
Method for enhancing control effect of biological control agent for plant parasitic nematodes Download PDFInfo
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- CN102090391A CN102090391A CN2010105930969A CN201010593096A CN102090391A CN 102090391 A CN102090391 A CN 102090391A CN 2010105930969 A CN2010105930969 A CN 2010105930969A CN 201010593096 A CN201010593096 A CN 201010593096A CN 102090391 A CN102090391 A CN 102090391A
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Abstract
The invention relates to a preparation method for enhancing the control effect of a biological control agent for plant parasitic nematodes, belonging to the technical field of biological pesticide. The method of the invention enhances the field survival rate, the anti-germination inhibiting effect and the anti-environment capability of the biological control agent by adding the biological control agent with sodium alginate, curdlan, carrageenin, starch or cyclodextrin cell protective agent, diatomite, crushed crop straw, porous grass carbon, porous vermiculite, porous activated carbon or perlite powder cell attachment agent, and polyvinyl alcohol, carrageenin or gelatin cell immobilizing agent, thereby improving the control effect of the biological control agent. According to the method, other properties, using way and preservation way of the biological control agent are not changed, the biological control agent can be used with other nematocide pesticides and plant growth regulating agents together, however, the mixed agent must be guaranteed to lead no side effect to the biological control agent. The method of the invention has the advantages of obviously stable control effect, good comprehensive property and great easiness for industrialized production.
Description
Technical field:
The present invention relates to a kind of method that improves plant nematode biological prevention and control agent preventive effect, belong to biological pesticide technical field.
Background technology:
Plant nematode is the plant disease that generally takes place in a kind of world wide, and only the known kind of root-knot nematode just reaches kind more than 70, and 3000 various plants that cause harm cause tremendous loss every year.Economic crops such as China plant nematode main harm tobacco, flowers, vegetables, cotton, soybean, peanut, pseudo-ginseng, American Ginseng, the loss that causes is very surprising.According to the incomplete statistics of national tobacco disease pest control information centre, the whole nation in 2000 only tobacco root-knot nematode onset area has reached 500,000 hectares, more than 500,000,000 yuan of direct economic loss.Only Yunnan Province's onset area just reaches 2.7 ten thousand hectares, about 100,000,000 yuan of direct economic loss, and indirect loss is even more serious.The Heilongjiang Province only the soybean Cyst nematode cause every year with a toll of 800,000,000 yuan more than.Pine wood nematode is called as smokeless forest fire, in Jiangsu, partly the area is serious Zhejiang, Guangdong, Shandong, Anhui, Hubei, Shanghai, Taiwan, Hong Kong etc. that the trend of stretching in the oriented whole nation takes place.In Shandong, vegetables main producing region such as Guangdong, Hainan and Yunnan, root knot nematode is in rising trend.Guizhou Province's American Ginseng have to stop production because of serious generation root-knot nematode.More seriously, cause the crop root disease seriously to take place because root-knot nematode is caused the crop wound after infecting.According to incompletely statistics, nematodiasis makes China's various crop annual production loss reach 10-15%, the whole world annual because of nematode cause with a toll of 1,000 hundred million dollars (Weischer, 1994).
The control of nematode at present mainly relies on chemical nematocide, but chemical nematocide mostly is the huge malicious agricultural chemicals of the natural disposition of going out, and not only develops immunity to drugs, and more causes environmental pollution, is detrimental to health.Have many disabled or be about to forbidding in succession.U.S.'s Celfume is stopped using respectively in 2005 in, methylene bromide in 2000.Gram has good preventive effect to nematode, toxicity height, destruction soil microflora though the upward a large amount of iron that use of production at present go out.The chemistry nematocide when ensureing agricultural production, has brought serious negative effect for human health and environment at the control eelworm harm.Because residue of pesticide exceed standard, commodity take place repeatedly for the tealeaves of China's export abroad, pseudo-ginseng, pear, Hami melon, fruit juice, flowers, honey and multiple animal products pays compensation for incident, directly influences the export of farm produce.The chemical nematocide of present domestic use simultaneously is mainly external imported product, and price is too expensive.Therefore, develop the nematode biological prevention and control agent and seem particularly important.
Occurring in nature, the natural enemy of nematode is a lot, mainly contains: fungi, bacterium, mite, predatism nematode etc.Wherein maximum is fungi, and research at present at most and what become launch also mainly is fungi.Mainly be divided into according to its characteristic that infects nematode:
1, predation fungus: catch nematode by the predacious organs that the nutrient hypha specialization forms;
2, parasitic fungus: by the fungus of the ovum of parasitic nematode, female worm, cyst etc.;
3, toxigenic bacterium thing: the fungus that can produce toxin poisoning nematode.
What become at present that product comes into the market mainly is predation fungus and parasitic fungus.
The nematode biological prevention and control agent just goes on the market as far back as eighties of last century the seventies.But through the development of decades, it uses and promotes still not extensively to today, comprises that a lot of countries of China do not use yet.Basic reason is the influence of ecotope, and wherein soil is one of most important influence factor to the inhibitory action that biocontrol microorganisms produces, and this also is that the numerous soil of restriction pass the bottleneck that class damage by disease and insect biocontrol microorganisms can not large-scale promotion.Because the existence of soil fungistasis, cause fungal spore germination rate in soil very low, generally between 0-40%, seldom surpass 50%, varying in size of dissimilar its mycostasises of soil finally causes biocontrol microorganisms to set up the population of different densities in soil, therefore same preparation occurred in different regions, the phenomenon that reflects different preventive effects in the dissimilar soil directly influences the application of biological prevention and control agent.The bottleneck that becomes restriction biocontrol fungicide preventive effect and use is sprouted in the field of microbial inoculum.
Summary of the invention:
The objective of the invention is to overcome the deficiency of prior art, a kind of method that improves plant nematode biological prevention and control agent preventive effect is provided.
Pin soil of the present invention improves biological prevention and control agent survival rate, anti-germination inhibitor and anti-environment capacity in the open air, thereby improves the biological prevention and control agent preventive effect by interpolation cell-protecting, cell attachment agent the bacteriostasis of nematode biological prevention and control agent.
The present invention realizes in the following manner:
1. biocontrol microorganisms is cultivated by conventional solid or liquid cultivating method, obtained to comprise mycelia, conidium, chlamydosporic liquid culture;
2. in liquid culture, add sodium alginate, heat polysaccharide, carragheen, starch or cyclodextrin cell polysaccharose protectant mixing with fixed attention, be incorporated as 1 ‰-1% of liquid culture;
3. add the porous cell adsorbent in above mixture, mixing is no less than one hour; The cell adsorbent is crop stalk, the porous peat composed of rotten mosses, porous vermiculite, porous activated carbon or the crushed crude pearlite of diatomite, pulverizing, and its adsorption capacity is that benchmark is 1 with water: 1.2-1.6, and particle size is 50-300 μ m;
4. continue in said mixture, to press 1 ‰ of mixed powder thing-7 ‰ and add polyvinyl alcohol, carragheen or gelatin cell fixation agent mixing, be no less than half an hour;
5. by the specific requirement drying of each biological prevention and control agent, pack, standby.
The present invention does not change other proterties, occupation mode, the preservation mode of biological prevention and control agent.The present invention improves preventive effect, activity, storage time, survival rate in the open air, anti-germination inhibitor and the anti-environment capacity of biological prevention and control agent.Can mix use with other nematicide agricultural chemicals, plant growth regulator, but must guarantee that mixed medicament has no side effect to biological prevention and control agent.
Method of the present invention can both arrive the effect that improves preventive effect to the living cells biological prevention and control agent, for different cytosiies certain difference is arranged, and has obviously stable control efficiency, and comprehensive proterties is good, is easy to suitability for industrialized production.
Embodiment:
Example one:
1. biocontrol microorganisms is cultivated by conventional liquid cultivating method, obtained to comprise mycelia, conidium, chlamydosporic liquid culture, the spore amount is determined by the specific requirement of each biological prevention and control agent;
2. in liquid culture, press 1 ‰ of liquid culture and add sodium alginate, mixing;
3. continuing to add adsorption capacity in above mixture is 1: 1.2 (is benchmark with water), and particle size is the diatomite of 50 μ m, and mixing is no less than one hour;
4. continue to press 1 ‰ of mixed powder thing and add polyvinyl alcohol in said mixture, mixing is no less than half an hour;
5. by the specific requirement drying of each biological prevention and control agent, pack, standby.
Example two:
1. biocontrol microorganisms is cultivated by conventional liquid cultivating method, obtained to comprise mycelia, conidium, chlamydosporic liquid culture, the spore amount is determined by the specific requirement of each biological prevention and control agent;
2. in liquid culture, press 5 ‰ of liquid culture and add heat polysaccharide with fixed attention, mixing;
3. continuing to add adsorption capacity in above mixture is 1: 1.4 (is benchmark with water), and particle size is the porous peat composed of rotten mosses of 200 μ m, and mixing is no less than one hour;
4. continue to press 4 ‰ of mixed powder thing and add polyvinyl alcohol in said mixture, mixing is no less than half an hour;
5. by the specific requirement drying of each biological prevention and control agent, pack, standby.
Example three:
1. biocontrol microorganisms is cultivated by conventional liquid cultivating method, obtained to comprise mycelia, conidium, chlamydosporic liquid culture, the spore amount is determined by the specific requirement of each biological prevention and control agent;
2. in liquid culture, press 1% of liquid culture and add carragheen, mixing;
3. continuing to add adsorption capacity in above mixture is 1: 6 (is benchmark with water), and particle size is the porous vermiculite of 300 μ m, and mixing is no less than one hour;
4. continue to press 7 ‰ of mixed powder thing and add polyvinyl alcohol in said mixture, mixing is no less than half an hour;
5. by the specific requirement drying of each biological prevention and control agent, pack, standby.
Example four:
1. biocontrol microorganisms is cultivated by conventional solid culture method, collection comprises mycelia, conidium, chlamydosporic culture, and thin up is to corresponding spore amount (the spore amount is determined by the specific requirement of each biological prevention and control agent);
2. in liquid culture, press 1% of liquid culture and add starch, mixing;
3. continuing to add adsorption capacity in above mixture is 1: 1.4 (is benchmark with water), and particle size is the crushed crude pearlite of 100 μ m, and mixing is no less than one hour;
4. continue to press 7 ‰ of mixed powder thing and add carragheen in said mixture, mixing is no less than half an hour;
5. by the specific requirement drying of each biological prevention and control agent, pack, standby.
Example five:
1. biocontrol microorganisms is cultivated by conventional liquid cultivating method, obtained to comprise mycelia, conidium, chlamydosporic liquid culture, the spore amount is determined by the specific requirement of each biological prevention and control agent;
2. in liquid culture, press 1% of liquid culture and add cyclodextrin cell, mixing;
3. continuing to add adsorption capacity in above mixture is 1: 1.6 (is benchmark with water), and particle size is the crop stalk of 300 μ m, and mixing is no less than one hour;
4. continue to press 7 ‰ of mixed powder thing and add gelatin in said mixture, mixing is no less than half an hour;
5. by the specific requirement drying of each biological prevention and control agent, pack, standby.
Example six:
1. biocontrol microorganisms is cultivated by conventional liquid cultivating method, obtained to comprise mycelia, conidium, chlamydosporic liquid culture, the spore amount is determined by the specific requirement of each biological prevention and control agent;
2. in liquid culture, press 1% of liquid culture and add sodium alginate, mixing;
3. continuing to add adsorption capacity in above mixture is 1: 1.6 (is benchmark with water), and particle size is the porous activated carbon of 300 μ m, and mixing is no less than one hour;
4. continue to press 7 ‰ of mixed powder thing and add gelatin in said mixture, mixing is no less than half an hour;
5. by the specific requirement drying of each biological prevention and control agent, pack, standby.
Embodiment seven: field test results
The control rate to root-knot nematode is used in biological prevention and control agent field after the processing
The result shows: the diseased plant rate/% of biological prevention and control agent, root knot percentage/%, the downright bad percentage/% of root, disease index are respectively than A Bading low 24.8%, 15%, 8%, 23.3, lower by 41.5%, 30%, 13%, 40 than blank, average preventive effect is than chemical pesticide A Badinggao 41%.The biological prevention and control agent of handling with the present invention is than former preparation, and the field germination rate of control efficiency, microbial inoculum has improved 15.5% and 10% respectively and shown the good preventive effect of biological prevention and control agent, has possessed to produce the primary condition that goes up large-scale popularization.
Claims (1)
1. method that improves plant nematode biological prevention and control agent preventive effect, the step of method that it is characterized in that improving plant nematode biological prevention and control agent preventive effect is as follows:
A. biocontrol microorganisms is cultivated by conventional solid or liquid cultivating method, obtained to comprise mycelia, conidium, chlamydosporic liquid culture;
B. in liquid culture, add sodium alginate or heat polysaccharide or carragheen or starch or cyclodextrin cell polysaccharose protectant mixing with fixed attention, be incorporated as 1 ‰-1% of liquid culture;
C. add the porous cell adsorbent in above mixture, mixing is no less than one hour; The cell adsorbent is crop stalk or the porous peat composed of rotten mosses or porous vermiculite or the porous activated carbon or the crushed crude pearlite of diatomite or pulverizing, and the adsorption capacity of adsorbent is that benchmark is 1 with water: 1.2-1.6, and particle size is 50-300 μ m;
D. continue in said mixture, to add polyvinyl alcohol, merit carragheen or gelatin cell fixation agent mixing, be no less than half an hour by 1 ‰ of mixed powder thing-7 ‰;
E. by the specific requirement drying of each biological prevention and control agent, pack, standby.
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| CN2010105930969A CN102090391A (en) | 2010-12-17 | 2010-12-17 | Method for enhancing control effect of biological control agent for plant parasitic nematodes |
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| CN2010105930969A CN102090391A (en) | 2010-12-17 | 2010-12-17 | Method for enhancing control effect of biological control agent for plant parasitic nematodes |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104247717A (en) * | 2013-06-25 | 2014-12-31 | 中国科学院沈阳应用生态研究所 | Microbial inoculant inhibiting diseases and insect pests of plants, and preparation method and application thereof |
| US11051460B2 (en) | 2015-01-19 | 2021-07-06 | Jiffy International As | Durable fiber plant growth containers and related materials and methods |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1984002829A1 (en) * | 1983-01-31 | 1984-08-02 | Termeloeszoevetkezetek Ertekes | Composition and process for the intensification of plant cultivation |
| CN1473475A (en) * | 2003-05-21 | 2004-02-11 | 云南大学 | Method for increasing growing and propagating ability of fungus agent in soil |
| CN101861880A (en) * | 2010-06-24 | 2010-10-20 | 云南大学 | Microbial agent and application thereof |
-
2010
- 2010-12-17 CN CN2010105930969A patent/CN102090391A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1984002829A1 (en) * | 1983-01-31 | 1984-08-02 | Termeloeszoevetkezetek Ertekes | Composition and process for the intensification of plant cultivation |
| CN1473475A (en) * | 2003-05-21 | 2004-02-11 | 云南大学 | Method for increasing growing and propagating ability of fungus agent in soil |
| CN101861880A (en) * | 2010-06-24 | 2010-10-20 | 云南大学 | Microbial agent and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 周峻沛等: "利用Brevibacillus laterosporus YMF3.00003菌株研制生物杀菌剂", 《云南大学学报(自然科学版)》, vol. 28, no. 5, 31 December 2006 (2006-12-31), pages 456 - 460 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104247717A (en) * | 2013-06-25 | 2014-12-31 | 中国科学院沈阳应用生态研究所 | Microbial inoculant inhibiting diseases and insect pests of plants, and preparation method and application thereof |
| US11051460B2 (en) | 2015-01-19 | 2021-07-06 | Jiffy International As | Durable fiber plant growth containers and related materials and methods |
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Application publication date: 20110615 |