CN102070410A - Microwave-combined enzymatic method for extracting resveratrol from giant knotweed rhizome - Google Patents

Abstract

The invention provides a microwave-combined enzymatic method for extracting resveratrol from giant knotweed rhizome, which comprises the following steps of: weighing a plurality of parts of 5.000g of giant knotweed rhizome, preparing into mixed solution in a liquid-to-material mass ratio of 20:1 by using 80 volume percent ethanol solution as an extracting agent, and adding 10mg of cellulase for enzymolysis under the optimal enzymatic extraction conditions, namely at the temperature of 50 DEG C and the pH value of 5.0 for 30 minutes, wherein a microwave-assisted extraction single factor experiment proves that microwave power has slight influence on the yield of the resveratrol, so only the influence of microwave time is considered; and performing microwave heating on the mixed solution subjected to enzymolysis under the power of 510W, performing suction-filtering on extracting solution under reduced pressure, concentrating, and drying at a low temperature to obtain a resveratrol sample. The resveratrol is extracted by the microwave-combined enzymatic method, so that on the one hand, the extraction ratio is greatly improved, and on the other hand, compared with a simple enzymatic extraction method, the microwave-combined enzymatic method greatly shortens extraction time; a simple microwave extraction method is high in speed but low in the extraction ratio; and the microwave-combined enzymatic method fully combines the advantages of the simple enzymatic extraction method and the simple microwave extraction method, so that production cost is reduced, and the method is more suitable for industrial production.

Description

Microwave is in conjunction with the method for trans-resveratrol in the Enzymatic Extraction giant knotweed

Technical field

The invention provides the method for a kind of microwave, relate to enzymolysis and extraction and extracting through microwave heating technology in conjunction with trans-resveratrol in the Enzymatic Extraction giant knotweed.

Technical background

Giant knotweed has another name called big tip of a leaf house steward; spot root Radix dactylicapni (Radix Dactylicapnotis); contain effective active matters such as anthraquinone class and stilbene compound in its root and the stem; trans-resveratrol (resveratrol) in middle its constituents of stilbene is a kind of important plant protecting chemical; can strengthen the ability of plant opposing pathogenic agent; and have anti-oxidant, remove free radical, prevent to wear out, antitumor, protection cardiovascular and the effect of phytoestrogen and the effect that protects the liver, be a kind of crude substance that exploitation is worth that has.

At present, have ten in the world surplus a countries and regions at exploitation trans-resveratrol raw material and preparation, be called the another new green cancer therapy drug after the taxol, by people's extensive concern.The domestic trans-resveratrol that mainly from giant knotweed, extracts, the organic solvent extraction of using more, and with the chloroform equal solvent as eluent, separate by silicagel column, required time is longer, and chloroform equal solvent toxicity is bigger, the dissolvent residual problem is difficult to solve, only the single component in the giant knotweed is carried out extraction separation, and can not make full use of the giant knotweed resource, economic worth is lower.The effective constituent of most of Chinese medicinal materials all is wrapped in the plant cell wall, with general organic solvent extraction of using, be difficult to destroy cell walls, extraction yield is lower, has the external eighties in 20th century to utilize enzymatic treatment to extract the report of natural product, studies show that, enzymolysis can make cell walls loosen, break, and reduces resistance to mass transfer, quickens effective constituent and discharges, thereby the raising extraction yield, the utility value of raising raw material.And microwave method have weak point heat-up time, homogeneous heating, quality volume production good, easily realize advantages such as automatization control.Microwave can directly act in molecule, makes the thermal motion aggravation of molecule, and causes that temperature raises, can the rapid damage cell walls from this heat effect, making in the herbal medicine imitate composition faster separation and Extraction come out.The present invention adopts enzyme, microwave acting in conjunction to extract the technology of trans-resveratrol down, enzyme and microwave and two kinds of methods is combined give full play to advantage separately, and this method extraction time is short, the extraction yield height, and good product quality realizes that easily automatization can be used for batch production production.

Summary of the invention

A kind of microwave is in conjunction with the method for trans-resveratrol in the Enzymatic Extraction giant knotweed, its difficult problem that mainly needs to solve be optimum enzymolysis and extraction condition, microwave extraction condition and both how in conjunction with the problem of extracting, the present invention has determined the optimum extraction condition of enzymolysis in conjunction with the microwave extraction trans-resveratrol by a series of experiments.

Example:

Take by weighing some parts of 5.000g Giant Knotweed Rhizome, with volume fraction is that 80% ethanolic soln is an extraction agent, being made into liquid material mass ratio and being 20: 1 mixed solution, is under 5.0 conditions in 50 ℃ of temperature, ph value promptly in the Enzymatic Extraction top condition, adds 10mg cellulase degradation 30min.Show that microwave power is little to the influence of trans-resveratrol yield because microwave-assisted extracts single factor experiment, therefore only consider the influence of microwave time.Microwave heating under the mixed solution 510W power that enzymolysis is crossed filters the extracting solution decompression, concentrates the back cryodrying, the trans-resveratrol sample.

Lime light: found through experiments microwave power and increase and to improve extract yield but influence is little, the too high container inner liquid medicine that causes easily of power acutely seethes with excitement, the ebullient medicine company can produce a large amount of foams, spray extraction vessel fast, cause loss of effective components, microwave should adopt intermittent action, with controlled temperature, prevents that soup from acutely seething with excitement.

The present invention adopts microwave to increase substantially extraction yield on the one hand in conjunction with the Enzymatic Extraction trans-resveratrol, compare greatly with simple Enzymatic Extraction on the other hand and shortened extraction time, though the simple microwave extraction time is fast but extraction yield is low, this law fully combines two kinds of method advantages, production cost is reduced, be more suitable in suitability for industrialized production.

Claims (5)

1. microwave is in conjunction with the method for trans-resveratrol in the Enzymatic Extraction giant knotweed, and its feature comprises following step:

(1) example:

Take by weighing some parts of 5.000g Giant Knotweed Rhizome, with volume fraction is that 80% ethanolic soln is an extraction agent, being made into liquid material mass ratio and being 20: 1 mixed solution, is under 5.0 conditions in 50 ℃ of temperature, ph value promptly in the Enzymatic Extraction top condition, adds 10mg cellulase degradation 30min.Show that microwave power is little to the influence of trans-resveratrol yield because microwave-assisted extracts single factor experiment, therefore only consider the influence of microwave time.Microwave heating under the mixed solution 510W power that enzymolysis is crossed filters the extracting solution decompression, concentrates the back cryodrying, the trans-resveratrol sample.

2. tell as claim (1), it is characterized in that adopting 80% ethanolic soln as the extraction agent best results.

3. tell as claim (1), it is characterized in that optimum extraction solid-liquid ratio selection 20: 1.

4. tell as claim (1), it is characterized in that having determined that enzymolysis process optimum extraction process condition is that 50 ℃ of temperature, ph values are under 5.0 conditions, add 10mg cellulase degradation 30min.。

5. tell as claim (1), it is characterized in that microwave power is 510W.The microwave power increase can improve extract yield but influence is little, and the too high container inner liquid medicine that causes easily of power acutely seethes with excitement, and the ebullient medicine company can produce a large amount of foams, the ejection extraction vessel causes loss of effective components fast, and microwave should adopt intermittent action, with controlled temperature, prevent that soup from acutely seething with excitement.

The present invention adopts microwave to increase substantially extraction yield on the one hand in conjunction with the Enzymatic Extraction trans-resveratrol, compare greatly with simple Enzymatic Extraction on the other hand and shortened extraction time, though the simple microwave extraction time is fast but extraction yield is low, this law fully combines two kinds of method advantages, production cost is reduced, be more suitable in suitability for industrialized production.