CN102049057A - Method for killing bacterial spores - Google Patents
Method for killing bacterial spores Download PDFInfo
- Publication number
- CN102049057A CN102049057A CN2010105937563A CN201010593756A CN102049057A CN 102049057 A CN102049057 A CN 102049057A CN 2010105937563 A CN2010105937563 A CN 2010105937563A CN 201010593756 A CN201010593756 A CN 201010593756A CN 102049057 A CN102049057 A CN 102049057A
- Authority
- CN
- China
- Prior art keywords
- concentration
- brood cell
- solution
- edta
- oxidant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for killing bacterial spores. The method comprises the following steps: 1) dropping L-alanine serving as a spore germinator on a contaminated article; 2) then continuing dropping a strong alkaline solution serving as a resistance weakening agent; and 3) adjusting the pH value of the solution to 4.0-5.0 by taking H2O2, Fe2<+> and EDTA as a reaction system so as to prepare an oxidant; and dropping the prepared oxidant on the contaminated article. The method provided by the invention is used for disinfecting the contaminated environment and articles and killing the bacterial spores attached to the environment and articles, and has the advantages of good effect, low toxicity, little stimulus, low cost and the like.
Description
Technical field
The present invention relates to a kind of method of sterilization, particularly a kind of effectively method of deactivation bacterial spore.
Technical background
Bacterial spore has the resistant function of height to physical and chemical factor.Therefore, conventional means was to take to burn to add high concentration chemicals long period of soaking when decontamination was carried out in the plague area due to the bacterial spore, but these methods are very serious to the pollution of environment.Carried out on-the-spot anthrax test as near the Gruinard island nineteen forty-two Scotland seashore, island are seriously polluted, just are purified after adopting high-concentration formaldehyde to add the sea water mixing immersion treatment in 1986; 2003, what the anthrax grave of Three Gorge Reservoir Region in Chongqing was handled also employing was that high power amount bleaching powder and mixing with soil are soaked, then the method for gasoline burning.
The characteristics that attack according to bio-terrorism attack ground often in the place of crowd's high concentration in recent years, as build in interior, the means of transport and important traffic place, on-the-spot decontamination difficulty and require to increase; It is very necessary to set up situ purification treatment technology reliable, environment-friendly type.
Summary of the invention
The method that the purpose of this invention is to provide a kind of kill bacteria brood cell, described method is utilized inducing of germinant and drag attenuator, promote bacterial spore to start rudiment, reduce the drag of cell space structure and composition, make that its chitin under the further effect of oxidant breaks, nucleolysis, thalline be by effectively deactivation.The present invention is used for contaminated environment and sterilisation of objects, kills the bacterial spore that is attached on environment and the article, and has effectively, and toxicity is low, and zest is little, low cost and other advantages.
Technical scheme of the present invention is:
Kill bacteria brood cell's method has following steps:
1) on contaminated article, drip spill concentration be 0.10-0.40 mol L as the L-alanine of brood cell's germinant, effect 20-60min;
2) subsequently, on the contaminated article of handling through the described brood cell's germinant of step 1), continue to drip spill concentration be 0.02-0.50 mol L as the strong base solution of drag attenuator, effect 20-40min;
3) with H
2O
2, Fe
2+, EDTA is reaction system, wherein H
2O
2Concentration be 0.10-0.80mol/L; Fe
2+Concentration be 0.01-0.08mol/L; The concentration of EDTA is 0.01-0.08mol/L; Get isopyknic H
2O
2, Fe
2+, EDTA solution is mixed, the pH value of adjusting solution is 4.0-5.0, promptly gets the preparation oxidant of being prepared; The oxidant of getting preparation drips and is sprinkled upon step 2) on the described contaminated article, action time 30-60min.
Step 2) described strong base solution is strong base solutions such as sodium hydroxide or potassium hydroxide.
Step 3) is described; Fe
2+Be ferrous sulfate or solution of ferrous chloride.
Reagent described in brood cell's germinant of the present invention, drag attenuator (basifier) and the oxidant is the commercially available prod.
Germinant, drag attenuator mainly act on brood cell's chitin and brood cell's clothing of bacterial spore among the present invention, and it is damaged that it is produced, and eliminates barrier and protective effect, and brood cell's core exposes; Oxidant can produce the hydroxyl radical free radical with forceful electric power position, and oxidant has the forceful electric power position, therefore strong oxidation is arranged, and germination body nucleolysis, enzymatic activity are disappeared, and thalline thoroughly is inactivated.
The method that the present invention is used for the kill bacteria brood cell is hypotoxicity to animal, and corrosivity is little, cost is low, and is easy to use.Each constituent is preserved at room temperature and is got final product, good stability, during use with joining with usefulness.
The specific embodiment
The present invention is described further below in conjunction with example.
1. results of laboratory
With final concentration be 0.10 mol L L-alanine be the germinant main constituent, effect brood cell suspension 20-60min.The sodium hydroxide aqueous slkali that with concentration is 0.02-0.50 mol L is the drag attenuator, acts on above-mentioned brood cell's suspension 20-40min, with H
2O
2, Fe
2+, EDTA is reaction system, determines H
2O
2Final concentration be 0.20mol/L, the concentration of ferrous sulfate is 0.02mol/L, the concentration of EDTA is 0.02mol/L, pH value 4.0-5.0, action time 30-60min.The result is as shown in table 1.
Brood cell's germinant of the present invention, drag attenuator (basifier) and oxidant, during use according to this method compound method, and according to sterilization steps of the present invention with joining with usefulness.
2. in-site modeling sterilization experiment
Get stone, tile, blade, cloth sheet, wooden unit surface (1x1cm area) drip brood cell's suspension and drip 10ul brood cell's suspension, get sandy soil 0.05g and drip 10ul brood cell's suspension, simulate contaminated external environment.Carry out brood cell's inactivation treatment with the inventive method:
With final concentration be 0.40 mol L L-alanine be germinant, drip 10ul in article surface, effect 20-60min;
The sodium hydroxide aqueous slkali that with concentration is 0.12-0.25 mol L is the drag attenuator, drips 90ul in above-mentioned article surface, effect 20-40min;
With H
2O
2, Fe
2+, EDTA is reaction system, determines H
2O
2Final concentration be 0.20mol/L, the concentration of ferrous sulfate is 0.02mol/L, the concentration of EDTA is 0.02mol/L, pH value in reaction 4.0-5.0 drips 90ul in above-mentioned article surface, effect 30-60min.
The result is carried out count plate.Different analog sample brood cell deactivation the results are shown in Table 2.
3. act on the mixed liquor toxicity test
Choose 60 of the SPF level Wistar rats of body weight 180-200 g, be divided into experimental group and matched group, male and female half and half.Acute oral toxicity test is pressed the 1.00ml/100g body weight, with gastric infusion of used sterilization mixed liquor, observes 14d record body weight, diet, death condition continuously; Subacute per os toxicity test is pressed the 1.00ml/100g body weight, and every day, per os was irritated the stomach contamination, and negative control group gives sterile distilled water.Continuous contamination 28d, last contamination back 24h broken end is got blood, detects every routine blood test index, and major organs is carried out pathologic finding.
The result: all in this detection unit range of normal value, every index of each dosage group and matched group compare every index, the equal not statistically significant of difference.This concentration sterilization mixed liquor is nontoxic.
Claims (3)
1. a kill bacteria brood cell method is characterized in that, following steps are arranged:
1) on contaminated article, drip spill concentration be 0.10-0.40 mol L as the L-alanine of brood cell's germinant, effect 20-60min;
2) subsequently, on the contaminated article of handling through the described brood cell's germinant of step 1), continue to drip spill concentration be 0.02-0.50 mol L as the strong base solution of drag attenuator, effect 20-40min;
3) with H
2O
2, Fe
2+, EDTA is reaction system, wherein H
2O
2Concentration be 0.10-0.80mol/L; Fe
2+Concentration be 0.01-0.08mol/L; The concentration of EDTA is 0.01-0.08mol/L; Get isopyknic H
2O
2, Fe
2+, EDTA solution is mixed, the pH value of adjusting solution is 4.0-5.0, promptly gets the preparation oxidant of being prepared; The oxidant of getting preparation drips and is sprinkled upon step 2) on the described contaminated article, action time 30-60min.
2. kill bacteria brood cell's according to claim 1 method is characterized in that: step 2) described strong base solution is strong base solutions such as sodium hydroxide or potassium hydroxide.
3. kill bacteria brood cell's according to claim 1 method, it is characterized in that: step 3) is described; Fe
2+Be ferrous sulfate or solution of ferrous chloride.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010593756 CN102049057B (en) | 2010-12-17 | 2010-12-17 | Method for killing bacterial spores |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010593756 CN102049057B (en) | 2010-12-17 | 2010-12-17 | Method for killing bacterial spores |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102049057A true CN102049057A (en) | 2011-05-11 |
| CN102049057B CN102049057B (en) | 2012-12-12 |
Family
ID=43954071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010593756 Expired - Fee Related CN102049057B (en) | 2010-12-17 | 2010-12-17 | Method for killing bacterial spores |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102049057B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102701881A (en) * | 2012-06-05 | 2012-10-03 | 凌天骏 | Soil disinfectant and soil disinfecting and sterilizing method |
| CN105962002A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院农产品加工研究所 | Method for killing bacterial spores in gentle manner |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6359004B1 (en) * | 1996-08-30 | 2002-03-19 | Duke University | Manipulating nitrosative stress to upregulate nitrosative stress defenses |
| CN1545883A (en) * | 2003-12-15 | 2004-11-17 | 韶山大北农动物药业有限公司 | Water soluble composite potassium monopersulfate powder disinfectant |
| JP4228175B2 (en) * | 2002-01-11 | 2009-02-25 | 三菱瓦斯化学株式会社 | Disinfectant composition liquid and disinfecting method |
| CN101698102A (en) * | 2009-05-13 | 2010-04-28 | 朱玛华 | Ultra-potent composite for decontaminating hazardous chemical substances |
-
2010
- 2010-12-17 CN CN 201010593756 patent/CN102049057B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6359004B1 (en) * | 1996-08-30 | 2002-03-19 | Duke University | Manipulating nitrosative stress to upregulate nitrosative stress defenses |
| JP4228175B2 (en) * | 2002-01-11 | 2009-02-25 | 三菱瓦斯化学株式会社 | Disinfectant composition liquid and disinfecting method |
| CN1545883A (en) * | 2003-12-15 | 2004-11-17 | 韶山大北农动物药业有限公司 | Water soluble composite potassium monopersulfate powder disinfectant |
| CN101698102A (en) * | 2009-05-13 | 2010-04-28 | 朱玛华 | Ultra-potent composite for decontaminating hazardous chemical substances |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102701881A (en) * | 2012-06-05 | 2012-10-03 | 凌天骏 | Soil disinfectant and soil disinfecting and sterilizing method |
| CN102701881B (en) * | 2012-06-05 | 2014-05-14 | 凌天骏 | Soil disinfectant and soil disinfecting and sterilizing method |
| CN105962002A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院农产品加工研究所 | Method for killing bacterial spores in gentle manner |
| CN105962002B (en) * | 2016-05-05 | 2019-07-09 | 中国农业科学院农产品加工研究所 | The method that mild formula kills bacterial spore |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102049057B (en) | 2012-12-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kumar et al. | A chronicle of SARS-CoV-2: seasonality, environmental fate, transport, inactivation, and antiviral drug resistance | |
| Mane et al. | A novel hybrid cavitation process for enhancing and altering rate of disinfection by use of natural oils derived from plants | |
| Alvear-Daza et al. | Resistance and induction of viable but non culturable states (VBNC) during inactivation of E. coli and Klebsiella pneumoniae by addition of H2O2 to natural well water under simulated solar irradiation | |
| UA53683C2 (en) | Bactericidal composition | |
| CN103922447A (en) | Novel environment-friendly bactericide composition and preparation and using method thereof | |
| CN103937701B (en) | Bacillus pumilus shou002 and application thereof | |
| CN105753110A (en) | Functional water and multiple application methods thereof | |
| CN103004897A (en) | Efficient biological bactericide | |
| CN101810198B (en) | Salicylic acid-iodine disinfector and preparation method thereof | |
| CN101578984A (en) | Algaecide combined by cinnamic aldehyde, eugenol and citric acid | |
| CN102049057B (en) | Method for killing bacterial spores | |
| June et al. | Copper and silver biocidal mechanisms, resistance strategies, and efficacy for legionella control | |
| CN103275909B (en) | Phthalate degrading bacteria and application thereof | |
| CN109370908A (en) | A kind of culture of chicken coccidial oocyst and save liquid and its application | |
| Stopforth et al. | Influence of extended acid stressing in fresh beef decontamination runoff fluids on sanitizer resistance of acid-adapted Escherichia coli O157: H7 in biofilms | |
| CN104211146A (en) | Method for inhibiting blue algae from growth by using plant fallen leaves | |
| CN102939966B (en) | A kind of compound disinfectant, its preparation method and application | |
| CN102747010B (en) | Allethrin degrading bacteria and soil restoration application thereof | |
| CN104188329B (en) | A kind of field survivorship purifying kettle | |
| Rogers | Experimental evaluation of mycoremediation of Escherichia coli bacteria in solution using Pleurotus ostreatus | |
| CN108130287B (en) | Application and preparation method of flavobacterium and secretion | |
| CN111759805A (en) | Oral and facial sterilizing and antiviral spray and preparation method and application thereof | |
| CN100536657C (en) | Composite efficient aldehyde insecticide | |
| CN104886110A (en) | Multipurpose composite cationic surfactant type disinfectant | |
| CN101123974A (en) | Silver-ionized plant extraction liquid and use thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121212 Termination date: 20141217 |
|
| EXPY | Termination of patent right or utility model |