JP4228175B2 - Disinfectant composition liquid and disinfecting method - Google Patents
Disinfectant composition liquid and disinfecting method Download PDFInfo
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Description
【０００１】
【発明の属する技術分野】
本発明は病院、食品工場、畜舎等の環境殺菌、及び医療機器、食品加工機器、調理器具、食器、手指等の殺菌に関するものである。
【０００２】
【従来の技術】
病院、食品工場、畜舎等の環境殺菌剤、医療機器、食品加工機器、調理器具、食器、手指等の殺菌剤としてこれまで種々の殺菌剤が開発されており、アルコール系、アルデヒド系、塩素系、カチオン系、両性界面活性剤系、ビグアナイド系、過酸化物系の殺菌剤を例示することができるが、以下の問題点がある。
【０００３】
すなわち、アルコール系殺菌剤は細菌芽胞に効果がない、揮発しやすいため残効性に乏しい等の欠点を有する。アルデヒド系の殺菌剤には毒性が高い、細菌芽胞、カビに対する効果が弱い等の問題がある。塩素系殺菌剤は結核菌、細菌芽胞に対する効果が弱い、有機物の存在下で速やかに分解し効力を失う、金属、木、ゴム、布等に対する腐食性が強い、臭気が強い、環境中でトリハロメタンを生成する等の問題を有している。
カチオン系殺菌剤は、結核菌、細菌芽胞に効果がない、カビ及びウィルスに対する効果が弱い、有機物の存在下で効力が低下する等の問題点がある。両性界面活性剤系殺菌剤は、結核菌、細菌芽胞、ウィルス、カビに対する効果が弱い。
ビグアナイド系殺菌剤は、結核菌、細菌芽胞、ウィルスに効果が弱い、耐性菌が生じる、菌株により効力に違いがある等の問題がある。
【０００４】
【発明が解決しようとする課題】
本発明の目的は、人体や環境に対する安全性に優れ、環境に優しく、しかも、細菌は勿論、細菌芽胞、酵母、カビ、ウィルス、原虫等を含む広範囲の微生物に対し強い殺菌効果を示す殺菌剤を提供することにある。
【０００５】
【課題を解決するための手段】
近年、本発明者らは上記諸問題に鑑み鋭意研究を重ねた結果、過酢酸とヨウ素分子（Ｉ2）を、特定の濃度範囲で共存させると、過酢酸とヨウ素分子の相乗作用により、顕著な殺菌力が発現することを見出し、この知見をもとに本発明を完成させた。
すなわち、本発明は、ヨウ素分子及び過酢酸を含む水溶液からなり、ヨウ素分子濃度が20〜200ppm、過酢酸濃度が50〜500ppmである殺菌剤組成液に関する。本発明の殺菌剤組成液は、物品を殺菌処理するために用いられる。
以下、本発明を詳細に説明する。
【０００６】
本発明の殺菌剤組成液は必須成分としてヨウ素分子及び過酢酸の両者を共に含む水溶液からなる。ヨウ素分子と過酢酸は、いずれも殺菌剤として知られているが、本発明においては、この両者が協同して殺菌作用を発揮し、相乗効果をもたらす。本発明の組成液は、酢酸及び過酸化水素を含んでもよい。また、これらの他に、pH調整剤、界面活性剤、着色剤、香料、他の殺菌成分等を含んでいてもよい。
本発明の組成液において、ヨウ素分子及び過酢酸は、水に溶解している。殺菌剤組成液中のヨウ素分子濃度は20〜200ppm、過酢酸濃度は50〜500ppmであり、好ましい濃度は、ヨウ素分子50〜100ppm、過酢酸100〜300ppmである。
ヨウ素分子及び過酢酸の少なくとも一方の濃度が、上記範囲を下回る場合には十分な相乗効果が得られず、同じく少なくとも一方の濃度が上記範囲を上回る場合には、ヨウ素分子もしくは過酢酸の臭気、又はヨウ素分子の着染性が強まり、品質的にも環境衛生的にも好ましくない。このように、ヨウ素分子及び過酢酸が、特定の濃度範囲で共存することにより、両成分の相乗作用が発現して、強い殺菌力を有しかつ人体や環境に対する安全性の面で優れた殺菌剤が得られる。
【０００７】
本発明の殺菌剤組成液は、ヨウ化物を含む水溶液Ａ液と、過酢酸を含む水溶液Ｂ液とを、所望により希釈水とともに、混合することによって得られる。過酢酸溶液に所定量の固体のヨウ素を添加し、本発明の殺菌剤組成物を調製しようとした場合、ヨウ素の溶解度が低いため、ヨウ素を溶解するのに長時間を要し実用的ではない。これに対し、本発明の過酢酸とヨウ素イオン(Ｉ-)の反応を利用した方法では、両水溶液を、必要に応じ水とともに希釈混合することにより、短時間で安全かつ容易に、必要量の本発明殺菌組成液を調製することができる。
【０００８】
本発明に用いる水溶液Ａ液に含まれるヨウ化物としては、水溶液中でヨウ素イオンを生成するものであれば良く、ヨウ化カリウム、ヨウ化ナトリウムに例示される金属ヨウ化物を挙げることができる。水溶液Ｂ液は、過酢酸の他に、酢酸及び過酸化水素を含んでもよい。
水溶液Ａ液及び水溶液Ｂ液は、両者を混合しなければ長期間安定に保存できる。水溶液Ａ液及び水溶液Ｂ液は、本発明殺菌剤組成物を必要とするまで、互いに接触又は混合しないような形で保存し、使用する時又は直前に混合される。
反応に用いる水溶液Ａ液中のヨウ化物濃度は1〜5％であり、好ましい濃度範囲は2〜4％である。水溶液Ｂ液における過酢酸は、2〜6％の濃度で、好ましくは3〜5％の濃度である。上記濃度範囲を下回る場合には、保存安定性の面で好ましくなく、逆に上記濃度範囲を上回る場合には、安全性の面で好ましくない。なお、水溶液Ｂ液に含まれる過酸化水素濃度を6％未満に保つことが、毒物及び劇物取締法上好ましい。
【０００９】
水溶液Ａ液と水溶液Ｂ液は、殺菌処理時又は殺菌処理前に、所望により希釈水とともに混合される。混合直後からヨウ素イオンと過酢酸の反応が開始され、速やかに強い殺菌力を示すヨウ素分子濃度に到達し、未反応の過酢酸とヨウ素分子からなる本発明殺菌剤組成液となる。なお、この反応に酵素は必要としない。
水溶液Ａ液と水溶液Ｂ液の混合比率は、過酢酸のモル数が、ヨウ素イオンのモル数の0.5倍超、好ましくは1〜10倍の範囲内、より好ましくは2〜8倍の範囲内になるように希釈混合する。 ヨウ素イオンのモル数に対する過酢酸のモル数の比率が上記範囲を下回る場合、ヨウ素分子へ酸化されないヨウ素イオンが残存し、ヨウ素イオンが有効に利用されない。また、該モル比が上記範囲を上回る場合には、過酢酸が余剰となり安全上好ましくなく経済的にも不利となる。
【００１０】
【発明の実施の形態】
本発明は、また、物品の殺菌方法に関する。
すなわち、本発明は、ヨウ化物を含む水溶液Ａ液と、過酢酸を含む水溶液Ｂ液とを希釈水とともに混合して、ヨウ素分子及び過酢酸を含む水溶液からなる殺菌剤組成液を得、当該組成液を物品に接触させることを特徴とする、物品の殺菌方法に関する。
【００１１】
ヨウ化物を含む水溶液Ａ液と過酢酸を含む水溶液Ｂ液は、殺菌処理前に混合して本発明の殺菌剤組成液とし、殺菌対象物品に接触せしめる。
【００１２】
殺菌対象物品に制限はなく、病院、食品工場、畜舎、医療機器、食品加工機器、調理器具、食器、手指等が挙げられる。本発明の殺菌剤組成液を対象物品に接触させる手段に制限はなく、散布、清拭、浸漬等が挙げられる。
【００１３】
【実施例】
本発明を実施例により具体的に説明するが、本発明は以下の実施例に限定されるものではない。
実施例１
4.5％過酢酸水溶液、2％ヨウ化カリウム水溶液それぞれ0.5mlを、水200mlに添加混合し、1分間、15℃に保った。その結果、過酢酸100ppm及びヨウ素分子35ppmを含む殺菌剤組成液が調製された。
10mlの普通ブイヨン培地またはポテト・デキストロース培地を入れた試験管に、大腸菌(Escherichia coli)、黄色ブドウ球菌(Staphylococcus aureus)、サルモネラ菌(Salmonella enteritidis)または酵母菌(Saccharomyces cerevisiae)を1白金耳植菌し、30℃でOD610が約1.0になるまで振とう培養した。それを同量の50mMリン酸バッファー(pH6.0)で洗浄、懸濁し、試験菌液とした。前記殺菌剤組成液に１/100容の試験菌液を加えて処理液とし、30℃で静置し、0.25分、0.5分、1分、2分、5分、15分、30分及び60分後に経時的に該処理液をサンプリングし、50mMリン酸バッファー(pH6.0)で希釈後、生菌数を測定した。生菌数が検出限界(5/ml)以下になるのに要した時間を殺菌時間として求めた。その結果を表1に示す。本発明の殺菌剤組成液は、供試した3種の細菌及び1種の酵母菌に対し、後記した比較例で使用した殺菌液と比べ強い殺菌力を示した。
【００１４】
比較例１〜３
過酢酸とヨウ素分子を含む液に替えて、過酢酸100ppm液(比較例１)、 ヨウ素分子35ppm液(比較例２)、又は、次亜塩素酸ナトリウム液(有効塩素濃度100ppm、比較例３)を使用した他は、実施例１と同様にして殺菌時間を求めた。その結果を表1に示す。
【００１５】
実施例２
4.5％過酢酸水溶液、2％ヨウ化カリウム水溶液それぞれ0.5mlを水100mlに添加混合し、1分間、15℃に保った。その結果、過酢酸200ppm及びヨウ素分子75ppmを含む殺菌剤組成液が調製された。
ポテト・デキストロース寒天斜面培地にクロカビ(Aspergillus niger)またはアオカビ(Penicillium chrysogenum)を接種し、25℃、1週間培養した。これに50mMリン酸バッファー(pH6.0)を入れ、攪拌することにより胞子の懸濁液を調整した。これをガーゼでろ過し菌糸の断片を除き、試験胞子液とした。前記殺菌剤組成液に１/100容の試験胞子液を加え、30℃で静置し、0.25分、0.5分、1分、5分、15分、30分及び60分後に処理液をサンプリングし、50mMリン酸バッファー(pH6.0)で希釈後、ポテト・デキストロース寒天平板培地に塗末し生存胞子数を測定した。生存胞子数が検出限界(5/ml)以下になるのに要した時間を殺菌時間として求めた。その結果を表２に示す。
本発明の殺菌剤組成液は、後記した比較剤で使用した殺菌液に比べ、クロカビ及びアオカビに対し、強い効力を示した。
【００１６】
比較例４〜６
過酢酸とヨウ素分子を含む液に替えて、過酢酸200ppm液(比較例４)、ヨウ素分子75ppm液(比較例５)、又は、次亜塩素酸ナトリウム液(有効塩素濃度150ppm、比較例６)を使用した他は、実施例２と同様にして、殺菌時間を求めた。その結果を表２に示す。
【００１７】
実施例3
普通ブイヨン寒天斜面培地にセレウス菌(Bacillus cereus)または枯草菌(Bacillus subtilis)を植菌し、30℃で７日間培養して芽胞を形成させた。それを50mMリン酸バッファー(pH6.0)中に懸濁し、熱処理により栄養細胞を殺滅したものを試験菌液とした。実施例２で調製した過酢酸200ppm及びヨウ素分子75ppmを含む殺菌剤組成液に１/100容の試験菌液を加え、30℃で静置し、0.5分、1分、5分、10分、15分、30分及び60分後に処理液をサンプリングし、適宜希釈後、普通寒天培地に塗末し生菌数を測定した。生菌数が検出限界(5/ml)以下になった時間を殺菌時間として求めた。その結果を表３に示す。
本発明の殺菌剤組成液は後記した比較例で使用した殺菌液と比べセレウス菌芽胞及び枯草菌芽胞に対し強い殺菌効力を示した。
【００１８】
比較例７〜９
過酢酸とヨウ素分子を含む液に替えて、過酢酸200ppm液(比較例７)、ヨウ素分子75ppm液(比較例８)、又は、次亜塩素酸ナトリウム液(有効塩素濃度150ppm、比較例９)を使用した他は、実施例３と同様にして殺菌時間を求めた。その結果を表３に示す。
【００１９】
実施例4
実施例２で調製した過酢酸200ppm及びヨウ素分子75ppmを含む殺菌剤
組成液20mlと、エコーウィルス液またはポリオウィルス液（約4000TCD
50/ml）5mlと混合後、30℃で静置し、0.5分、1分、5分、15分、30分及び60分後に0.1Nチオ硫酸ナトリウムにより活性成分を不活性化した。該処理液0.1mlをサンプリングし、HeLa細胞を培養した試験管5本に添加した。6日間観察を続け、CPE(細胞変性作用)の阻止の有無を検定した。すべての試験管でCPE阻止が観察された場合をウィルス不活化作用が認められたものと判断して殺菌時間として求めた。その結果を表４に示す。
本発明の殺菌剤組成液は、後記した比較例で使用した殺菌剤に比べ供試したウィルスに対し強い不活化作用を示した。
【００２０】
比較例１０〜１２
過酢酸とヨウ素分子を含む液に替えて、過酢酸200ppm液(比較例１０)、ヨウ素分子75ppm液(比較例１１)、又は、次亜塩素酸ナトリウム液(有効塩素濃度150ppm、比較例１２)を使用した他は、実施例４と同様にして殺菌時間を求めた。その結果を表４に示す。
【００２１】
実施例5
実施例２で調製した過酢酸200ppm及びヨウ素分子75ppmを含む殺菌剤組成液を醗酵食品工場の床に1m2あたり1.5リットル散布した。散布5分後に水洗し、滅菌綿棒を用いたふきとり法（10cm×10cm）により生菌数を測定した。その結果を表5に示す。
本発明の殺菌剤組成液は、実際使用場面においても後記した比較例で使用した殺菌剤に比べ強い殺菌効果を示した。
【００２２】
比較例１３〜１５
過酢酸とヨウ素分子を含む液に替えて、過酢酸200ppm液(比較例１３)、ヨウ素分子75ppm液(比較例１４)、又は、次亜塩素酸ナトリウム液(有効塩素濃度150ppm、比較例１５)を使用した他は、実施例5と同様にして生菌数を求めた。その結果を表5に示す。
【００２３】
【発明の効果】
本発明の殺菌剤組成物は、人体や環境に対する安全性に優れ、しかも、細菌は勿論、細菌芽胞、酵母、カビ、ウィルス、原虫等を含む広範囲の微生物に対し強い殺菌効果を示す。また、本発明の殺菌方法は、効果的かつ簡便な物品の殺菌方法である。
【表１】
BACKGROUND OF THE INVENTION
The present invention relates to environmental sterilization of hospitals, food factories, barns and the like, and sterilization of medical equipment, food processing equipment, cooking utensils, tableware, fingers and the like.
[0002]
[Prior art]
Various disinfectants have been developed as disinfectants for hospitals, food factories, barns, etc., medical devices, food processing equipment, cooking utensils, tableware, fingers, etc., alcohol-based, aldehyde-based, chlorine-based Cationic, amphoteric surfactant-based, biguanide-based and peroxide-based bactericides can be exemplified, but have the following problems.
[0003]
That is, the alcohol-based disinfectant has disadvantages such as being ineffective on bacterial spores and being easily volatilized and thus having poor residual effect. Aldehydic germicides have problems such as high toxicity and weak effects on bacterial spores and molds. Chlorine disinfectant has a weak effect on M. tuberculosis and bacterial spores, quickly decomposes and loses its effectiveness in the presence of organic substances, is highly corrosive to metals, wood, rubber, cloth, etc., has a strong odor, and is a trihalomethane in the environment Has a problem of generating.
Cationic fungicides have problems such as ineffectiveness against tuberculosis bacteria and bacterial spores, weak effects on molds and viruses, and reduced efficacy in the presence of organic substances. Amphoteric surfactant fungicides are less effective against Mycobacterium tuberculosis, bacterial spores, viruses and molds.
The biguanide fungicides have problems such as weak effects on tuberculosis bacteria, bacterial spores and viruses, the generation of resistant bacteria, and differences in efficacy depending on the strain.
[0004]
[Problems to be solved by the invention]
An object of the present invention is an antibacterial agent that is excellent in safety to the human body and the environment, is friendly to the environment, and exhibits a strong bactericidal effect against a wide range of microorganisms including bacteria, bacteria, spores, fungi, viruses, protozoa, etc. Is to provide.
[0005]
[Means for Solving the Problems]
In recent years, the present inventors have conducted extensive research in view of the above problems, and as a result, when peracetic acid and iodine molecules (I 2 ) coexist in a specific concentration range, The present invention was completed based on this finding.
That is, the present invention relates to a bactericide composition liquid comprising an aqueous solution containing iodine molecules and peracetic acid, having an iodine molecule concentration of 20 to 200 ppm and a peracetic acid concentration of 50 to 500 ppm. The disinfectant composition liquid of the present invention is used for sterilizing articles.
Hereinafter, the present invention will be described in detail.
[0006]
The bactericide composition liquid of the present invention comprises an aqueous solution containing both iodine molecules and peracetic acid as essential components. Both iodine molecules and peracetic acid are known as bactericides, but in the present invention, both cooperate to exert a bactericidal action and bring about a synergistic effect. The composition liquid of the present invention may contain acetic acid and hydrogen peroxide. In addition to these, a pH adjuster, a surfactant, a colorant, a fragrance, other sterilizing components, and the like may be included.
In the composition liquid of the present invention, iodine molecules and peracetic acid are dissolved in water. The concentration of iodine molecules in the bactericide composition solution is 20 to 200 ppm, and the concentration of peracetic acid is 50 to 500 ppm. Preferred concentrations are 50 to 100 ppm of iodine molecules and 100 to 300 ppm of peracetic acid.
When the concentration of at least one of iodine molecules and peracetic acid is below the above range, a sufficient synergistic effect cannot be obtained. Similarly, when at least one concentration exceeds the above range, the odor of iodine molecules or peracetic acid, Or the dyeing | staining property of an iodine molecule becomes strong and is unpreferable also in terms of quality and environmental hygiene. In this way, the coexistence of iodine molecules and peracetic acid in a specific concentration range allows the synergistic action of both components to develop and has a strong bactericidal power and is excellent in terms of safety to the human body and the environment. An agent is obtained.
[0007]
The bactericide composition liquid of the present invention can be obtained by mixing an aqueous solution A containing iodide and an aqueous solution B containing peracetic acid together with dilution water as desired. When a predetermined amount of solid iodine is added to a peracetic acid solution to prepare the disinfectant composition of the present invention, the solubility of iodine is low, so it takes a long time to dissolve iodine and is not practical. . On the other hand, in the method using the reaction of peracetic acid and iodine ion (I − ) of the present invention, both aqueous solutions are diluted and mixed with water as needed, so that the required amount can be safely and easily in a short time. The sterilizing composition liquid of the present invention can be prepared.
[0008]
The iodide contained in the aqueous solution A used in the present invention is not particularly limited as long as it generates iodine ions in the aqueous solution, and examples thereof include metal iodides exemplified by potassium iodide and sodium iodide. The aqueous solution B may contain acetic acid and hydrogen peroxide in addition to peracetic acid.
The aqueous solution A and the aqueous solution B can be stored stably for a long period of time unless both are mixed. The aqueous solution A and the aqueous solution B are stored in such a manner that they do not come into contact with or mix with each other until the fungicide composition of the present invention is required, and are mixed when or just before use.
The iodide concentration in the aqueous solution A used for the reaction is 1 to 5%, and the preferred concentration range is 2 to 4%. Peracetic acid in the aqueous solution B has a concentration of 2 to 6%, preferably 3 to 5%. When the concentration is below the above range, it is not preferable in terms of storage stability, and conversely, when it is above the above range, it is not preferable from the viewpoint of safety. In addition, it is preferable on the poisonous and deleterious substances control law to maintain the hydrogen peroxide concentration contained in the aqueous solution B below 6%.
[0009]
The aqueous solution A and the aqueous solution B are mixed with diluting water as desired before or during the sterilization treatment. Immediately after mixing, the reaction between iodine ions and peracetic acid is started, the iodine molecule concentration that exhibits a strong bactericidal power is quickly reached, and the present bactericidal composition solution comprising unreacted peracetic acid and iodine molecules is obtained. Note that no enzyme is required for this reaction.
The mixing ratio of the aqueous solution A and the aqueous solution B is such that the number of moles of peracetic acid is more than 0.5 times the number of moles of iodine ions, preferably 1 to 10 times, more preferably 2 to 8 times. Dilute to mix. When the ratio of the number of moles of peracetic acid to the number of moles of iodine ions is below the above range, iodine ions that are not oxidized into iodine molecules remain, and iodine ions are not effectively used. On the other hand, when the molar ratio exceeds the above range, peracetic acid is excessive, which is not preferable for safety and economically disadvantageous.
[0010]
DETAILED DESCRIPTION OF THE INVENTION
The present invention also relates to a method for sterilizing an article.
That is, the present invention mixes an aqueous solution A containing iodide and an aqueous solution B containing peracetic acid together with dilution water to obtain a bactericide composition solution comprising an aqueous solution containing iodine molecules and peracetic acid. The present invention relates to a method for sterilizing an article, wherein a liquid is brought into contact with the article.
[0011]
The aqueous solution A containing iodide and the aqueous solution B containing peracetic acid are mixed before the sterilization treatment to obtain the sterilizing agent composition liquid of the present invention, and are brought into contact with the article to be sterilized.
[0012]
There are no restrictions on articles to be sterilized, and examples include hospitals, food factories, barns, medical equipment, food processing equipment, cooking utensils, tableware, and fingers. There is no restriction | limiting in the means to which the bactericidal agent composition liquid of this invention is made to contact target object, A spraying, wiping, immersion, etc. are mentioned.
[0013]
【Example】
EXAMPLES The present invention will be specifically described with reference to examples, but the present invention is not limited to the following examples.
Example 1
0.5 ml each of a 4.5% aqueous solution of peracetic acid and a 2% aqueous solution of potassium iodide were added to and mixed with 200 ml of water and kept at 15 ° C. for 1 minute. As a result, a bactericidal composition liquid containing 100 ppm peracetic acid and 35 ppm iodine molecules was prepared.
Inoculate a test tube containing 10 ml of normal bouillon medium or potato dextrose medium with 1 platinum ear inoculation of Escherichia coli, Staphylococcus aureus, Salmonella enteritidis or yeast (Saccharomyces cerevisiae). The culture was performed at 30 ° C. until the OD610 was about 1.0. It was washed and suspended with the same amount of 50 mM phosphate buffer (pH 6.0) to obtain a test bacterial solution. 1/100 volume test bacteria solution is added to the bactericide composition solution to make a treatment solution, which is allowed to stand at 30 ° C., 0.25 min, 0.5 min, 1 min, 2 min, 5 min, 15 min, 30 min and 60 After a minute, the treatment solution was sampled over time, diluted with 50 mM phosphate buffer (pH 6.0), and the viable cell count was measured. The time required for the viable count to fall below the detection limit (5 / ml) was determined as the sterilization time. The results are shown in Table 1. The bactericidal composition liquid of the present invention showed stronger bactericidal power than the bactericidal liquids used in the comparative examples described later against the three types of bacteria and one type of yeast tested.
[0014]
Comparative Examples 1-3
Instead of a solution containing peracetic acid and iodine molecules, peracetic acid 100 ppm solution (Comparative Example 1), iodine molecule 35 ppm solution (Comparative Example 2), or sodium hypochlorite solution (effective chlorine concentration 100 ppm, Comparative Example 3) The sterilization time was determined in the same manner as in Example 1 except that was used. The results are shown in Table 1.
[0015]
Example 2
0.5 ml each of a 4.5% peracetic acid aqueous solution and a 2% potassium iodide aqueous solution were added to and mixed with 100 ml of water, and kept at 15 ° C. for 1 minute. As a result, a bactericide composition liquid containing 200 ppm peracetic acid and 75 ppm iodine molecules was prepared.
Potato dextrose agar slope medium was inoculated with black mold (Aspergillus niger) or green mold (Penicillium chrysogenum) and cultured at 25 ° C. for 1 week. To this, 50 mM phosphate buffer (pH 6.0) was added and stirred to prepare a spore suspension. This was filtered with gauze to remove mycelial fragments and used as a test spore solution. Add 1/100 volume test spore solution to the above bactericide composition solution, leave it at 30 ° C, and sample the treatment solution after 0.25 min, 0.5 min, 1 min, 5 min, 15 min, 30 min and 60 min. Then, after dilution with 50 mM phosphate buffer (pH 6.0), it was applied to a potato-dextrose agar plate medium and the number of viable spores was measured. The time required for the number of viable spores to fall below the detection limit (5 / ml) was determined as the sterilization time. The results are shown in Table 2.
The bactericidal composition liquid of the present invention showed a stronger effect on black mold and blue mold than the bactericidal liquid used in the comparative agents described later.
[0016]
Comparative Examples 4-6
Instead of a solution containing peracetic acid and iodine molecules, a 200 ppm solution of peracetic acid (Comparative Example 4), a 75 ppm solution of iodine molecules (Comparative Example 5), or a sodium hypochlorite solution (effective chlorine concentration 150 ppm, Comparative Example 6) The sterilization time was determined in the same manner as in Example 2 except that was used. The results are shown in Table 2.
[0017]
Example 3
Bacillus cereus or Bacillus subtilis was inoculated on a normal bouillon agar slope medium, and cultured at 30 ° C. for 7 days to form spores. A suspension of this in 50 mM phosphate buffer (pH 6.0) and killed vegetative cells by heat treatment was used as a test bacterial solution. 1/100 volume of the test bacterial solution is added to the bactericide composition solution containing 200 ppm peracetic acid and 75 ppm iodine molecule prepared in Example 2, and left at 30 ° C. for 0.5 minutes, 1 minute, 5 minutes, 10 minutes, After 15 minutes, 30 minutes and 60 minutes, the treatment solution was sampled, diluted appropriately, and spread on a normal agar medium, and the viable cell count was measured. The time when the viable cell count was below the detection limit (5 / ml) was determined as the sterilization time. The results are shown in Table 3.
The bactericide composition liquid of the present invention showed stronger bactericidal efficacy against Bacillus cereus and Bacillus subtilis spores than the bactericidal liquid used in Comparative Examples described later.
[0018]
Comparative Examples 7-9
Instead of a solution containing peracetic acid and iodine molecules, a 200 ppm solution of peracetic acid (Comparative Example 7), a 75 ppm solution of iodine molecules (Comparative Example 8), or a sodium hypochlorite solution (effective chlorine concentration 150 ppm, Comparative Example 9) The sterilization time was determined in the same manner as in Example 3 except that was used. The results are shown in Table 3.
[0019]
Example 4
20 ml of a bactericide composition solution containing 200 ppm of peracetic acid and 75 ppm of iodine molecules prepared in Example 2, and an echovirus solution or poliovirus solution (about 4000 TCD)
After mixing with 5 ml of 50 / ml), the mixture was allowed to stand at 30 ° C., and the active ingredient was inactivated with 0.1N sodium thiosulfate after 0.5 min, 1 min, 5 min, 15 min, 30 min and 60 min. 0.1 ml of the treatment solution was sampled and added to five test tubes in which HeLa cells were cultured. Observation was continued for 6 days, and the presence or absence of inhibition of CPE (cytopathic action) was assayed. When CPE inhibition was observed in all the test tubes, it was determined that the virus inactivating action was observed and was determined as the sterilization time. The results are shown in Table 4.
The bactericide composition liquid of the present invention showed a strong inactivation effect on the virus tested compared to the bactericides used in the comparative examples described later.
[0020]
Comparative Examples 10-12
Instead of a solution containing peracetic acid and iodine molecules, peracetic acid 200 ppm solution (Comparative Example 10), iodine molecule 75 ppm solution (Comparative Example 11), or sodium hypochlorite solution (effective chlorine concentration 150 ppm, Comparative Example 12) The sterilization time was determined in the same manner as in Example 4 except that was used. The results are shown in Table 4.
[0021]
Example 5
The fungicidal composition liquid containing 200 ppm of peracetic acid and 75 ppm of iodine molecules prepared in Example 2 was sprayed on the floor of the fermented food factory at 1.5 liters per 1 m 2 . Five minutes after spraying, the cells were washed with water, and the viable cell count was measured by a wiping method (10 cm × 10 cm) using a sterile cotton swab. The results are shown in Table 5.
The bactericide composition liquid of the present invention showed a stronger bactericidal effect than the bactericides used in the comparative examples described later even in actual use situations.
[0022]
Comparative Examples 13-15
Instead of a solution containing peracetic acid and iodine molecules, peracetic acid 200 ppm solution (Comparative Example 13), iodine molecule 75 ppm solution (Comparative Example 14), or sodium hypochlorite solution (effective chlorine concentration 150 ppm, Comparative Example 15) The viable cell count was determined in the same manner as in Example 5 except that was used. The results are shown in Table 5.
[0023]
【The invention's effect】
The disinfectant composition of the present invention is excellent in safety to the human body and the environment, and exhibits a strong disinfecting effect against a wide range of microorganisms including bacteria, spore, yeast, mold, virus, protozoa and the like. The sterilization method of the present invention is an effective and simple method for sterilizing articles.
[Table 1]
Claims (2)
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| JP2002072492A JP4228175B2 (en) | 2002-01-11 | 2002-03-15 | Disinfectant composition liquid and disinfecting method |
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| CN102049057A (en) * | 2010-12-17 | 2011-05-11 | 中国人民解放军第三军医大学 | Method for killing bacterial spores |
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| RU2486908C1 (en) * | 2011-11-16 | 2013-07-10 | Государственное научное учреждение Курский научно-исследовательский институт агропромышленного производства | Method for preparing antiseptic preparation with metabolic and hepatoprotective activity |
| JP2014051304A (en) * | 2012-09-07 | 2014-03-20 | Dainippon Printing Co Ltd | Method and apparatus for antiseptic sealing of sterile chamber |
| EP2799068A1 (en) * | 2013-05-01 | 2014-11-05 | National University of Ireland, Galway | Antimicrobial compositions and methods for their production |
| US20190144313A1 (en) * | 2017-11-16 | 2019-05-16 | Peroxychem Llc | Disinfection method for water and wastewater |
| CN112535681B (en) * | 2019-09-23 | 2024-01-23 | 华南理工大学 | Arylsulfatase and glucuronidase inhibitor, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102049057A (en) * | 2010-12-17 | 2011-05-11 | 中国人民解放军第三军医大学 | Method for killing bacterial spores |
| CN102049057B (en) * | 2010-12-17 | 2012-12-12 | 中国人民解放军第三军医大学 | Method for killing bacterial spores |
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