CN102048696A - Preparation method of lipid nanoparticles - Google Patents
Preparation method of lipid nanoparticles Download PDFInfo
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- CN102048696A CN102048696A CN2011100068975A CN201110006897A CN102048696A CN 102048696 A CN102048696 A CN 102048696A CN 2011100068975 A CN2011100068975 A CN 2011100068975A CN 201110006897 A CN201110006897 A CN 201110006897A CN 102048696 A CN102048696 A CN 102048696A
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Abstract
The invention discloses a preparation method of lipid nanoparticles, comprising the following steps: dissolving solid lipid or a mixture of the solid lipid and liquid lipid in an organic solvent which is mutually soluble with water to form an oil phase, wherein surface active agent can be dissolved into the oil phase, and also can be dissolved into an aqueous phase; and pressurizing to 0.1-100MPa to lead the oil phase to pass through a microporous membrane to be dispersed into the moving aqueous phase to form the lipid nanoparticles, wherein, the aperture of the microporous membrane is 0.01-10 mu m. The preparation method disclosed by the invention has the following technical effects of having moderate preparation condition, avoiding the operations, such as heating and the like, having simple equipment process, less operation steps and capability of continuous production, and being suitable for large-scale production of medicine and fine chemical engineering enterprises.
Description
Technical field
The present invention relates to a kind of preparation method of lipid nanoparticle.
Background technology
Lipid nanoparticle is a kind of novel carriers drug-supplying system that grows up in generation nineteen ninety, it is that mixture with natural or synthetic solid-state lipid or solid-state lipid and operative liquid lipid is a carrier material, with medicine absorption or be wrapped in the lipid core, the particle diameter of formation is the solid nano drug-supplying system of 50 ~ 1000 nm.
Solid lipid nanoparticle (solid lipid nanoparticles, SLN) be first generation lipid nanoparticle, its preparation material is for belonging to solid-state matrix material (not containing liquid lipid) under room temperature and body temperature state, its weak point is pure solid lipid material degree of crystallinity height, and Gui Ze crystal leaves that the space of medicine is less, drug loading is low, medicine leaks easily at lay up period for relatively.(nanostructured lipid carriers is the second filial generation lipid nanoparticle NLC) to nano structured lipid carrier, adds the matrix material of operative liquid during preparation in solid-state lipid, and mixing lipid still is solid-state under room temperature and body temperature state.Nano structured lipid carrier is the physical stability height not only, and drug loading is big, and can control the release of medicine.
Lipid nanoparticle has many advantages: slow controlled release; Targeting; The medicine that the protection parcel advances in the lipid core is not degraded; Envelop rate is higher; Can wrap up fat-soluble medicine and the water soluble drug of making fat-soluble prodrug; The matrix material physiological compatibility is good, and is biodegradable, and toxicity is low; Can be mass-produced etc.
Lipid nano particle preparation is suitable for oral administration, intravenously administrable, pulmonary administration, and multiple route of administration such as dosing eyes and local skin administration are a kind of drug administration carriers with vast potential for future development.Aspect local skin administration and cosmetics, lipid nanoparticle has remarkable advantages, the suspension of lipid nanoparticle can join in the traditional Emulsion and gel, the low particle size of lipid nanoparticle has film forming performance on skin, can reduce the evaporation of moisture of skin, make skin become tender beautiful in vain.The lipid nanoparticle of carrying medicament can be positioned epidermis and dermal sites for a long time, reaches the drug level and the prolonging drug action time that improve local skin.Lipid nanoparticle can also protect entrapped active substance to avoid chemolysis, improves stability of drug.
Reported the preparation method of multiple lipid nanoparticle at present in the document, as fusion-homogenize method method, cooling-homogenize method, microemulsion method etc.These methods all have its limitation, all need experience pyroprocess as fusion-homogenize method method, cooling-homogenize method, microemulsion method, are not suitable for the lipid nanoparticle preparation to thermo-responsive medicine; Microemulsion method dosage of surfactant is bigger, untoward reaction such as easy toxigenicity.The present invention has overcome the limitation of said method; under the lower condition of temperature, can prepare lipid nanoparticle; the preparation condition gentleness; avoid high-temperature operation, be fit to heat sensitive material, do not need to use a large amount of surfactants during preparation; apparatus and process is simple; operating procedure is few, and seriality is strong, is suitable for medicine and the large-scale production of fine chemistry industry enterprise.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of lipid nanoparticle, the lipid nanoparticle mean diameter of this method preparation is less than 300nm, the particle size distribution homogeneous.
The present invention is achieved like this, and method is: the mixture of solid-state lipid or solid-state lipid and liquid lipid is dissolved in the organic solvent formation oil phase that dissolves each other with water, and surfactant dissolves in oil phase, also dissolves in water, pressurization 0.1
~It is the aqueous phase that the microporous membrane of 0.01 ~ 10 m is scattered in motion by the aperture that 100Mpa makes oil phase, forms lipid nanoparticle.
Described solid-state lipid is a kind of or some kinds of mixture in stearic acid, Palmic acid, mountain Yu acid, trilaurin, three Semen Myristicae acid glycerides, tripalmitin, glyceryl tristearate, glyceryl monostearate, glycerol distearate, three Yu acid glycerides, cholesterol, the spermaceti; Described liquid lipid is a kind of or some kinds of mixture among oleic acid, capric acid, caprylic/capric triglyceride, octadecanol, Labrafac CC, the Migyol 812.
Described surfactant is that poloxamer, natural or synthetic phospholipid, Polysorbate, fatty acid Pyrusussuriensis are smooth, one or more mixture of polyoxyethylene aliphatic alcohol ether, polyoxyethylene fatty acid ester, fatty glyceride, sodium lauryl sulphate, soap, alevaire, sodium cholate, sodium glycocholate, sodium taurocholate, sodium taurodeoxycholate.
The organic solvent that dissolves each other with water is the mixed solvents of a kind of or some kinds of compositions in ethanol, acetone, propanol, isopropyl alcohol, the tert-butyl alcohol, Polyethylene Glycol, the glycerol.
Described microporous membrane is ceramic membrane, glass-film, polycarbonate membrane or cellulose membrane.
By the material of matrix material load can be medicine, health product or skin care material.
Technique effect of the present invention is: the preparation condition gentleness, avoided operations such as high temperature, and apparatus and process is simple, and operating procedure is few, but continuous production is suitable for medicine and the large-scale production of fine chemistry industry enterprise.In addition, as adopting the organic solvent of ethanol as the lipin dissolving material, when producing cosmetics, can remove, the lipid nanoparticle suspension of Xing Chenging can join in the traditional Emulsion and gel at last; As adopt the organic solvent of the tert-butyl alcohol as the lipin dissolving material, and the suspension for preparing is carried out lyophilizing, can obtain freeze-dried powder, convenient transportation stores and uses.
The specific embodiment
Further specify this method below in conjunction with concrete embodiment, but protection scope of the present invention is not limited in this, protection scope of the present invention sees claims.
Embodiment 1
Prescription:
Stearic acid 0.5(w/w)
Tween 80 0.3(w/w)
Epikuron?200 0.2（w/w）
Ethanol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 1m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The stearic acid, Tween 80, the phospholipid (Epikuron 200) that take by weighing recipe quantity are dissolved in ethanol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 30 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 169nm, and polydispersity index is 0.190.
Embodiment 2
Prescription:
Stearic acid 0.5(w/w)
Poloxamer 188 0.3(w/w)
Epikuron?200 0.2（w/w）
Ethanol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 1m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The stearic acid, poloxamer 188, the Epikuron 200 that take by weighing recipe quantity are dissolved in ethanol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 30 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 183nm, and polydispersity index is 0.181.
Embodiment 3
Prescription:
Glyceryl monostearate 0.5(w/w)
Tween 80 0.3(w/w)
Epikuron?200 0.2（w/w）
Ethanol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 1m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The glyceryl monostearate, Tween 80, the Epikuron 200 that take by weighing recipe quantity are dissolved in ethanol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 30 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 186nm, and polydispersity index is 0.183.
Embodiment 4
Prescription:
Glyceryl monostearate 0.5(w/w)
Poloxamer 188 0.3(w/w)
Epikuron?200 0.2（w/w）
Ethanol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 1m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The glyceryl monostearate, poloxamer 188, the Epikuron 200 that take by weighing recipe quantity are dissolved in ethanol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 30 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 183nm, and polydispersity index is 0.175.
Embodiment 5
Prescription:
Glyceryl monostearate 0.5(w/w)
Poloxamer 188 0.3(w/w)
Epikuron?200 0.2（w/w）
Isopropyl alcohol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 1m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The glyceryl monostearate, poloxamer 188, the Epikuron 200 that take by weighing recipe quantity are dissolved in isopropyl alcohol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 40 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 150nm, and polydispersity index is 0.167.
Embodiment 6
Prescription:
Glyceryl monostearate 0.5(w/w)
Poloxamer 188 0.3(w/w)
Epikuron?200 0.3（w/w）
Isopropyl alcohol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 5m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The glyceryl monostearate, poloxamer 188, the Epikuron 200 that take by weighing recipe quantity are dissolved in ethanol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 40 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 157nm, and polydispersity index is 0.154.
Embodiment 7
Prescription:
Glyceryl monostearate 1.0(w/w)
Tween 80 0.5(w/w)
Epikuron?200 0.5（w/w）
Acetone 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 5m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The stearic acid, Tween 80, the Epikuron 200 that take by weighing recipe quantity are dissolved in ethanol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 40 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 187nm, and polydispersity index is 0.195.
Embodiment 8
Prescription:
Glyceryl monostearate 0.5(w/w)
Tween 80 0.3(w/w)
Epikuron?200 0.2（w/w）
Tert-butyl alcohol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 8m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The glyceryl monostearate, Tween 80, the Epikuron 200 that take by weighing recipe quantity are dissolved in the tert-butyl alcohol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 40 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 179nm, and polydispersity index is 0.183.
Embodiment 9
Prescription:
Glyceryl monostearate 0.5(w/w)
Poloxamer 188 0.3(w/w)
Epikuron?200 0.3（w/w）
Tert-butyl alcohol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 5m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The glyceryl monostearate, poloxamer 188, the Epikuron 200 that take by weighing recipe quantity are dissolved in the tert-butyl alcohol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 40 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 175nm, and polydispersity index is 0.167.
Embodiment 10
Prescription:
Glyceryl monostearate 1.0(w/w)
Tween 80 0.5(w/w)
Epikuron?200 0.5（w/w）
Tert-butyl alcohol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 8m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The glyceryl monostearate, Tween 80, the Epikuron 200 that take by weighing recipe quantity are dissolved in the tert-butyl alcohol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 40 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 187nm, and polydispersity index is 0.191.
Embodiment 11
Prescription:
Palmic acid 0.5(w/w)
Tween 80 0.3(w/w)
Epikuron?200 0.2（w/w）
Ethanol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 5m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The Palmic acid, Tween 80, the Epikuron 200 that take by weighing recipe quantity are dissolved in ethanol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 40 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 178nm, and polydispersity index is 0.187.
Embodiment 12
Prescription:
Glyceryl tristearate 0.5(w/w)
Tween 80 0.3(w/w)
Epikuron?200 0.2（w/w）
Isopropyl alcohol 10(v/w)
Water adds to 100
Preparation technology: the water of recipe quantity is joined in the container, open water pump, make that water is 8m/s by the speed of ceramic membrane, water flows at the pipeline internal recycle.The glyceryl tristearate, Tween 80, the Epikuron 200 that take by weighing recipe quantity are dissolved in isopropyl alcohol as oil phase together, oil phase is joined in the ceramic membrane disperser, adopting pressure is the nitrogen pressure of 0.3Mpa, making oil phase is the ceramic membrane of 0.1 μ m by the aperture, mechanical agitation speed in the container is 300rpm, and the temperature of water and oil phase all remains on 30 ℃.
Physicochemical characteristics: collect the solid lipid nanoparticle suspension of the above-mentioned preparation of 10ml, carry out particle diameter and particle size distribution.The mean diameter of gained solid lipid nanoparticle is 176nm, and polydispersity index is 0.184.
Claims (6)
1. the preparation method of a lipid nanoparticle is characterized in that method is: the mixture of solid-state lipid or solid-state lipid and liquid lipid is dissolved in the organic solvent that dissolves each other with water constitutes oil phase, surfactant dissolves in oil phase, also dissolves in water, pressurization 0.1
~It is the aqueous phase that the microporous membrane of 0.01 ~ 10 m is scattered in motion by the aperture that 100Mpa makes oil phase, forms lipid nanoparticle.
2. the preparation method of a kind of lipid nanoparticle according to claim 1, it is characterized in that: described solid-state lipid is a kind of or some kinds of mixture in stearic acid, Palmic acid, mountain Yu acid, trilaurin, three Semen Myristicae acid glycerides, tripalmitin, glyceryl tristearate, glyceryl monostearate, glycerol distearate, three Yu acid glycerides, cholesterol, the spermaceti; Described liquid lipid is a kind of or some kinds of mixture among oleic acid, capric acid, caprylic/capric triglyceride, octadecanol, Labrafac CC, the Migyol 812.
3. the preparation method of a kind of lipid nanoparticle according to claim 1 is characterized in that: described surfactant is that poloxamer, natural or synthetic phospholipid, Polysorbate, fatty acid Pyrusussuriensis are smooth, one or more mixture of polyoxyethylene aliphatic alcohol ether, polyoxyethylene fatty acid ester, fatty glyceride, sodium lauryl sulphate, soap, alevaire, sodium cholate, sodium glycocholate, sodium taurocholate, sodium taurodeoxycholate.
4. the preparation method of a kind of lipid nanoparticle according to claim 1 is characterized in that: the organic solvent that dissolves each other with water is a kind of or some kinds of mixed solvents of forming in ethanol, acetone, propanol, isopropyl alcohol, the tert-butyl alcohol, Polyethylene Glycol, the glycerol.
5. the preparation method of a kind of lipid nanoparticle according to claim 1, it is characterized in that: described microporous membrane is ceramic membrane, glass-film, polycarbonate membrane or cellulose membrane.
6. the preparation method of a kind of lipid nanoparticle according to claim 1 is characterized in that by the material of matrix material load can be medicine, health product or skin care material.
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| JP7048496B2 (en) | 2015-09-17 | 2022-04-05 | アラヤ （シャンハイ） バイオサイエンス カンパニー リミテッド | Highly stable non-vesicular nanoparticles and their use in the treatment of microbial infections |
| CN106539758A (en) * | 2015-09-17 | 2017-03-29 | 阿赖耶识（上海）生物技术有限公司 | A kind of non-vesicle type nano particle of high stable and its application in treatment infection of staphylococcus aureus |
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| CN111759823B (en) * | 2015-09-17 | 2022-12-30 | 阿赖耶识（上海）生物技术有限公司 | High-stability non-vesicular nanoparticles and application thereof in treating microbial infection |
| JP2018532770A (en) * | 2015-09-17 | 2018-11-08 | アラヤ （シャンハイ） バイオサイエンス カンパニー リミテッド | Highly stable non-vesicular nanoparticles and their use in the treatment of microbial infections |
| EP3351238A4 (en) * | 2015-09-17 | 2019-07-10 | Alaya (Shanghai) Bioscience Co., Ltd | Highly stable non-vesicular nanoparticles and application thereof in treating microbial infection |
| CN106551902B (en) * | 2015-09-17 | 2020-07-03 | 阿赖耶识（上海）生物技术有限公司 | High-stability non-vesicular nanoparticles and application thereof in treating microbial infection |
| CN111759823A (en) * | 2015-09-17 | 2020-10-13 | 阿赖耶识（上海）生物技术有限公司 | High-stability non-vesicular nanoparticles and application thereof in treating microbial infection |
| AU2016321503B2 (en) * | 2015-09-17 | 2022-04-14 | Alaya (Shanghai) Bioscience Co., Ltd | Highly stable non-vesicular nanoparticles and application thereof in treating microbial infection |
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| CN105326812A (en) * | 2015-10-28 | 2016-02-17 | 南昌大学 | Sorafenib solid lipid nanoparticles and preparation method thereof |
| CN107019682B (en) * | 2017-04-13 | 2019-09-03 | 中国药科大学 | A kind of Nimodipine lipid nanoparticle and its preparation process |
| CN107019682A (en) * | 2017-04-13 | 2017-08-08 | 中国药科大学 | A kind of Nimodipine lipid nanoparticle and its preparation technology |
| CN113784707A (en) * | 2018-11-05 | 2021-12-10 | 沃特福德技术学院 | Artificial tear |
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| CN102048696B (en) | 2013-08-07 |
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