CN102029163B - Catalase catalyst analog as well as preparation method and application thereof - Google Patents
Catalase catalyst analog as well as preparation method and application thereof Download PDFInfo
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- CN102029163B CN102029163B CN200910272236XA CN200910272236A CN102029163B CN 102029163 B CN102029163 B CN 102029163B CN 200910272236X A CN200910272236X A CN 200910272236XA CN 200910272236 A CN200910272236 A CN 200910272236A CN 102029163 B CN102029163 B CN 102029163B
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention provides a catalase catalyst analog which is bismuth ferrite. The preparation method comprises the following steps of: dissolving raw materials of ferric nitrate and bismuth nitrate in ethylene glycol monomethyl ether; adding a complexant and a dispersant to prepare sol and further obtain gel; calcining the obtained gel at high temperature; and postprocessing the obtained gel to obtain BiFeO3 catalase catalyst analog. The catalase catalyst analog has simple preparation process, low cost and stable chemical activity. Because the catalase catalyst analog can be subjected to magnetic recovery, the catalase catalyst can be reused many times, thereby overcoming the defects that the traditional catalase catalyst analog is synthesized in a mode wastes time, labor and money, is unstable and is hard to recover during application and the like. The catalase catalyst analog can serve as the substitute product of the natural catalase, can be used for detecting and analyzing H2O2 content in food samples, biological samples and environment samples and can be used for degrading and mineralizing organic pollutants.
Description
Technical field
The present invention relates to the quasi-enzyme catalytic field, particularly many ferrimagnets ferrous acid bismuth (BiFeO
3) the catalatic catalytic activity of type of having, it can be under near neutral, room temperature and the no special external energy condition of (referring to ultrasonic, heating using microwave, high light irradiation etc.) effective activation hydrogen peroxide (H
2O
2) produce the hydroxy radical of strong oxidizing property, thus make organic pollutant degradation and mineralising, and the detection and the water that can be applicable to content of hydrogen peroxide pollute processing.
Technical background
Environmental pollution particularly water pollution is one of focal issue of restriction China economic development.Microbial method is modal sewage water treatment method, but owing to contain a large amount of high toxicities, the organic pollution of difficult degradation in many industrial wastewaters, can cause the microorganism can't normal growth even death and losing efficacy when handling this type waste water with traditional microbial method.In recent years, people attempt the optically catalytic TiO 2 technology is used to handle industrial wastewater, but because existing photocatalysis technology exists utilization ratio of optical energy and the low inferior deficiency of quantum efficiency, thereby seriously limited its actual application value.
Classical Fenton reaction (Fe
2+And H
2O
2) can produce a large amount of hydroxy radicals with strong oxidizing property ability, be widely used in the processing of waste water.But also there are some problems in the method in practical application, as Fenton reaction, only is applicable to pH less than 3 acid system, and Fe
2+Be depleting agents, cause the generation of a large amount of iron mud, thereby make that last handling process is an effort again consuming time.And then people's various new improvement technology that begins one's study.Utilize ultraviolet light or direct current to quicken Fe if any the people
3+Be converted into Fe
2+, so that improve the cyclic utilization rate of Fenton reaction, but this light Fenton or Fenton technology still exist in actual application problems such as energy consumption is big, disposal cost height.Zhao advances just to wait people (Ji, H.; Song, W.; Chen, C.; Yuan, H.; Ma, W.; Zhao, J.Environ.Sci.Technol.2007,41,5103-5107.) with organic ligand and Fe
3+Or Fe
2+Coordination generates organic coordination compound, and these organic coordination compounds are immobilized on ion exchange resin.Utilize it that absorbing of visible light (or sunshine) realized Fe
3+To Fe
2+Conversion, but this system still need consume external energy, keeps Fe
3+With Fe
2+Circulation, reach effective activation H
2O
2Purpose.
Since the eighties in last century, have discover in vital movement with the ferro element be the catalase of activated centre atom can be under room temperature, no external energy and nearly neutral condition activation H
2O
2Yet, time-consuming, the effort, expensive of these natural catalatic purge processes, and its environment tolerance is very limited, in use and easy inactivation, thereby can not directly be used for waste water control.With Meunier be representative Environmental Chemistry man be devoted to develop native catalase always substitute be used for activation H
2O
2, they have developed a kind of ferrous phthalocyanine complex, can be in the mixed solvent of acetonitrile and water under room temperature condition effective activation H
2O
2, and degraded (Sorokin, the J.L. of realization chlorophenol pollutant; Seris, J.L.; Meunier, B.Science 1995,268,1163-1165.).But if make water make solvent separately, the degraded of chlorophenols will obviously be suppressed, thereby limit this system in the actual environment Application of Sewage.People such as American scholar Collins have developed a kind of huge ring organic coordination compound of tetramine base (commodity are called Fe-TAML) of iron, this catalyst can be under the condition of the aqueous solution, nearly neutral pH, room temperature and no external energy activation H efficiently
2O
2, and can be with the rapid mineralising of organic pollution (Collins, T.J.Acc.Chem.Res.2002,35,782-790 and Gupta, S.S.; Stadler, M.; Noser, C.A.; Ghosh, A.; Steinhoff, B.; Lenoir, D.; Horwitz, C.P.; Schramm, K.; Collins, T.J.Science 2002,296,326-328.).Though this type of catalyst shows excellent performance aspect catalytic activity; But its preparation process is complicated, cost is very high, be difficult to recycling; And this catalyst is originally as organic compound; The hydroxy radical oxidation that is produced in can be in the process of degraded and inactivation by system, even the dissociation of ligand reaction takes place under lower pH condition and lost efficacy (Polshin, V.; Popescu, D.L.; Fischer, A.; Chanda, A.; Homer, D.C.; Beach, E.S.; Henry, J.; Qian, Y.L.; Horwitz, C.P.; Lente, G.; Fabian, I.; Munck, E.; Bominaar, E.L.; Ryabov, A.D.; Collins, T.J.J.Am.Chem.Soc.2008,130,4497-4506.).Therefore, develop the class hydrogen peroxide enzyme catalyst that the gentle anti-hydroxy radical ability of a kind of service condition is strong, the preparation method simple, be easy to recycle, have crucial economy and social value aspect industry and the sanitary wastewater improvement.
Summary of the invention
Task of the present invention provides a kind catalase catalyst; It is very simple and reliable to make it have the preparation process, but low in raw material cost is easy to get, chemism stablize magnetic recovery, can be at the aqueous solution, room temperature, near neutral and do not introduce effective activation H under any special external energy condition of (referring to ultrasonic, heating using microwave, high light irradiation etc.)
2O
2Produce the characteristics such as hydroxy radical of strong oxidizing property, with overcome prior art effort, time-consuming, expensive and native catalase or existing type hydrogen peroxide catalyzed dose when the synthetic type of hydrogen peroxide enzyme catalyst in use unstable, be difficult to deficiency such as recovery.
Realize that technical scheme of the present invention is:
This kind catalase catalyst provided by the invention is the ferrous acid bismuth, and what the ferrous acid bismuth was used during as class catalase catalyst applications is ferrous acid bismuth meal end.
The present invention with the ferrous acid bismuth as class catalase catalyst activation H
2O
2,, thereby realize H in food, biology or the environmental sample in order to the hydroxy radical oxidizing ferment substrate of generation strong oxidizing property
2O
2Content detection is analyzed, and described zymolyte is benzoic acid, cumarin, P-hydroxybenzoic acid, aniline, o-phenylenediamine or 3,3,5,5-tetramethyl benzidine etc.
The present invention with the ferrous acid bismuth as class hydrogen peroxide enzyme activation H
2O
2Hydroxy radical to produce strong oxidizing property is realized oxidative degradation and mineralising to environmental organic pollutant; Said here environmental organic pollutant comprises: type organic pollution such as organic dyestuff, endocrine interferon, antibiotic and machine chloro pesticide, and wherein organic dyestuff comprises materials such as rhodamine, methyl orange, malachite green and methylene blue; The endocrine interferon comprises materials such as nonyl phenol, bisphenol-A and estrone; Antibiotic comprises materials such as tetracycline, terramycin and chloramphenicol; Organo-chlorine pesticide comprises materials such as hexachloro-benzene, toxaphene and DDT.
Provided by the invention type of catalase Preparation of catalysts method may further comprise the steps:
(1) presses Fe (NO
3)
39H
2O and Bi (NO
3)
35H
2The mol ratio of O is that 1: 1 ratio is dissolved in it in EGME solution, with EGME solution and used Fe (NO
3)
39H
2The mol ratio of the iron atom among the O is 8～160: 1, is preferably 10～30: 1, and more preferably 20: 1 ratio is confirmed the consumption of EGME solution, is to stir 5～60min under 25～60 ℃ of conditions in temperature, with 0.1～6mol L
-1HNO
3The pH to 4 of regulator solution～7;
(2) in the solution that step (1) obtains, add complexant, with complexant and the used Fe (NO of step (1)
3)
39H
2The mol ratio of the iron atom among the O is 0.1～5: 1, is preferably 1～3: 1, and more preferably 1: 1 ratio is confirmed the consumption of complexant, adds dispersant again, with dispersant and the used Fe (NO of step (1)
3)
39H
2The mol ratio of iron atom is 5～100: 1 among the O; Be preferably 10～20: 1; More preferably 15: 1 ratio is confirmed the consumption of dispersant, under temperature is 25～150 ℃, stirs 10～100min and obtains pitchy colloidal sol, under 100 ℃ of conditions, keeps 5～40h to obtain gel then;
(3) the gel roasting 5～50min on electric furnace that step (2) is obtained transfers to then in the Muffle furnace and to be 400～600 ℃ in temperature and to keep obtaining in 2～10 hours graininess class catalase catalyst B iFeO down
3
With the prepared graininess class of step (3) catalase catalyst B iFeO
3Obtain Powdered type of catalase catalyst B iFeO through grinding
3, can be during concrete operations with the prepared graininess BiFeO of step (3)
3Class hydrogen peroxide enzyme catalyst places mortar, promptly obtains Powdered BiFeO through repeatedly grinding
3Class hydrogen peroxide enzyme catalyst.
The complexant that adds in the above-mentioned steps (2) is any one or two kinds in oxalic acid, ethylenediamine tetra-acetic acid (EDTA), nitrilotriacetic acid (NTA), ethylenediamine, bipyridyl and the Phen, and when the complexant that adds was two kinds, the ratio between them was arbitrarily.The dispersant that adds in the above-mentioned steps (2) is a kind of in ethylene glycol, polyethylene glycol and the glycerine or two kinds, and when the dispersant that adds was two kinds, the ratio between them was arbitrarily.
The powdery product that has confirmed the present invention's preparation through Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction characterization methods such as (XRD) is BiFeO really
3, seeing Fig. 1 and Fig. 2, Fig. 1 is X-ray diffraction (XRD) collection of illustrative plates of the pulverulent solids product of the inventive method preparation for Fourier transform infrared spectroscopy (FT-IR) figure of the powdery product of the inventive method preparation, Fig. 2.As can beappreciated from fig. 1, the prepared product of the present invention is at 553 and 443 centimetres
-1The place has two tangible infrared absorption peaks, represents the stretching vibration of Fe-O and the flexural vibrations of O-Fe-O respectively.As can beappreciated from fig. 2, the prepared product of the present invention has typical perovskite structure, does not have the generation of iron oxide and bismuth oxide dephasign, its result and BiFeO
3Standard x RD spectrogram (JCPDS File No.20-169) unanimity proves that the product that the present invention makes is BiFeO really
3
Record its intensity of magnetization-magnetic field intensity (M-H) curve through magnetometer, obtaining its saturation magnetization is 2.2 electromagnetic unit/gram (emu g
-1); See Fig. 3, Fig. 3 is the magnetic hysteresis regression curve collection of illustrative plates of the powdery product of the inventive method preparation, and as can beappreciated from fig. 3, its saturation magnetization of the prepared product of the present invention is 2.2emu g
-1, after externally-applied magnetic field removes, its intensity of magnetization will be classified as zero, explain that the prepared product of the present invention has superparamagnetism.
BiFeO of the present invention
3The class catalase can replace native catalase (like the horseradish catalase) to be used for H
2O
2Detection.Particularly, BiFeO of the present invention
3Class catalase powder is activation H effectively
2O
2Produce the hydroxy radical of strong oxidizing property; And can be fast with multiple colourless hydrogen peroxide zymolyte (as 3,3,5; The 5-tetramethyl benzidine; O-phenylenediamine, aniline etc.) oxidation generates the product with different colours, or not fluorescent zymolyte (like cumarin, bipyridyl, benzoic acid etc.) oxidation is generated fluorescence-causing substance.Utilize the changing value and the H of these substrates absorbance or fluorescence intensity in the fermentoid oxidation reaction process
2O
2There is linear relationship between the concentration, can be used for H in food, environment and the biological sample
2O
2The check and analysis of content.
Because BiFeO of the present invention
3Type catalase can be in room temperature, near neutral and do not introduce under any special external energy situation of (referring to ultrasonic, heating using microwave, high light irradiation etc.) effectively activation H
2O
2Produce the hydroxy radical of strong oxidizing property, thereby can be fast and degraded even the various toxic organic pollutants of mineralising effectively.These toxic pollutants comprise the organic dyestuff (like rhodamine, methyl orange, methylene blue etc.) of various bio-refractories, endocrine interferon (like nonyl phenol, bisphenol-A, estrone etc.) and organo-chlorine pesticide (like hexachloro-benzene, toxaphene, DDT etc.), antibiotic (like tetracycline, terramycin, chloramphenicol etc.) etc.
BiFeO provided by the invention
3Type hydrogen peroxide enzyme catalyst can and not introduced under any special external energy condition of (referring to ultrasonic, heating using microwave, high light irradiation etc.) effectively activation H in room temperature, nearly neutral pH
2O
2Produce the hydroxy radical of strong oxidizing property, the common zymolyte of hydroxy radical oxidation that can this strong oxidizing property is used for H
2O
2The check and analysis of content also can be widely used in the degraded and the mineralising of organic pollution in the environment.
BiFeO provided by the invention
3Class hydrogen peroxide enzyme catalyst can directly use commercially available ferric nitrate and bismuth nitrate to be raw material, and preparation technology is simple, does not have harsh synthesis condition requirement, and is with low cost.Reuse but synthetic catalyst is stable, nontoxic, application pH scope is wide, pollutant is had high clearance magnetic recovery.These advantages have guaranteed that such hydrogen peroxide enzyme catalyst can remove and the mineralising organic pollution efficiently, thus the environmental pollution of effectively administering these pollutants and being caused.
As everyone knows, native catalase is at catalytic activation H
2O
2The aspect has best catalytic activity; A class hydrogen peroxide enzyme catalyst can reach the catalytic activity identical with native catalase and the present invention makes, can be at the aqueous solution, room temperature, near neutral and do not introduce under any special external energy condition of (referring to ultrasonic, heating using microwave, high light irradiation etc.) effectively activation H
2O
2Produce the hydroxy radical of strong oxidizing property, thus degraded and the various organic pollutions of mineralising fast.Therefore, BiFeO provided by the invention
3Type hydrogen peroxide enzyme catalyst is in the waste water control industry value that is widely used, and in addition, as the substitute products of native catalase, this type of enzyme catalyst also can be used for H in food, biology and the environmental sample
2O
2The check and analysis of content.
Description of drawings
Fig. 1 is the FT-IR collection of illustrative plates of the powdery product of the inventive method preparation, and its ordinate is a transmitance, abscissa be wave number (centimetre
-1).Can find out that from Fig. 1 the prepared product of the present invention is 553 and 443cm
-1Have two tangible infrared absorption peaks, represent the stretching vibration of Fe-O and the flexural vibrations of O-Fe-O respectively.
Fig. 2 is the XRD figure spectrum of the powdery product of the inventive method preparation, and its ordinate is a diffracted intensity, and abscissa is angle of diffraction (degree).Can find out that from Fig. 2 the prepared product of the present invention has typical perovskite structure, does not have the generation of iron oxide and bismuth oxide dephasign, its result and BiFeO
3Standard x RD spectrogram (JCPDS File No.20-169) unanimity proves that the product that the present invention synthesizes is BiFeO really
3
Fig. 3 is the magnetic hysteresis regression curve collection of illustrative plates of the powdery product of the inventive method preparation, and its ordinate is specific magnetization (electromagnetic unit/gram), and abscissa is magnetic field intensity (oersted).Can find out that from Fig. 3 its saturation magnetization of the prepared product of the present invention is 2.2emu g
-1, after externally-applied magnetic field removes, its intensity of magnetization will be classified as zero, explain that the prepared product of the present invention has superparamagnetism.
Fig. 4 is BiFeO of the present invention
3Class hydrogen peroxide enzyme activation H
2O
2Oxybenzene formic acid detects H
2O
2The fluorescence spectrum figure of content, ordinate are different H
2O
2The fluorescence intensity of the fluorescence-causing substance that is generated under the concentration; Abscissa is wavelength (nanometer), and (interior illustration is for measuring H
2O
2The calibration curve collection of illustrative plates, ordinate is the fluorescence intensity of the fluorescence-causing substance that generates; Abscissa is H
2O
2Concentration (every liter of micromole)).This figure explanation BiFeO of the present invention
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2, can be advantageously used in low concentration H
2O
2The detection of content.
Fig. 5 is BiFeO of the present invention
3Class hydrogen peroxide enzyme activation H
2O
2The ultraviolet-visible spectrogram that fades of degraded rhodamine, ordinate is an absorbance, and abscissa is wavelength (nanometer), and (interior illustration is different system oxidative degradation organic dyestuff rhodamines, the independent H of curve 1 representative
2O
2The degraded rhodamine, the independent BiFeO of curve 2 representatives
3The degraded rhodamine, curve 3 is represented BiFeO
3-H
2O
2System degraded rhodamine, ordinate are the dyestuff residual concentration and the ratio of its initial concentration; Abscissa is reaction time (branch)).The BiFeO that this figure explanation the present invention makes
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2, can be advantageously used in the rhodamine degraded of organic pollution in the environment.
The specific embodiment
Embodiment 1: use BiFeO of the present invention
3Class catalase catalyst activation H
2O
2Come Oxybenzene formic acid to be used for indirect determination H
2O
2Content
With the 5mg BiFeO that makes
3Pulverulent solids joins in the 10mL distilled water, and ultrasonic dispersion 1min is with isopyknic 1.0mmol L
-1The benzoic acid aqueous solution, add H again
2O
2(ultimate density is 2.0 * 10
-8～2.0 * 10
-5Mol L
-1).At this moment, the substrate benzoic acid can be generated the hyperfluorescence material by catalytic oxidation, and the maximum emission wavelength of its fluorescence-causing substance is 405nm.Behind reaction 15min, it is centrifugal to get the 2mL reactant liquor rapidly, and the supernatant after centrifugal is taken out, and on sepectrophotofluorometer, measures the fluorescence intensity of supernatant at the 405nm place.This BiFeO
3The changing value and the H of class catalase catalyst system and catalyzing fluorescence intensity at the 405nm place
2O
2Concentration is 2.0 * 10
-8～2.0 * 10
-5Mol L
-1Scope internal memory linear relationship is seen Fig. 4.In addition, we choose native catalase (is example with the horseradish catalase) as catalyst replacement BiFeO
3The class catalase repeats this experiment, and the result shows the changing value and the H of horseradish catalase catalyst system and catalyzing fluorescence intensity at the 405nm place
2O
2Concentration is 2.0 * 10
-8～2.0 * 10
-5Mol L
-1Also deposit linear relationship in the scope.The class hydrogen peroxide enzyme catalyst that this control experiment explanation the present invention makes can reach the catalytic activity identical with native catalase.Utilize BiFeO provided by the invention
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2Excellent properties, can be advantageously used in low concentration H in the actual samples such as food, environment and biology
2O
2The check and analysis of content.
Embodiment 2: use BiFeO of the present invention
3Class catalase catalyst activation H
2O
2Come the oxidation cumarin to be used for indirect determination H
2O
2Content
With the 5mg BiFeO that makes
3Pulverulent solids joins in the 10mL distilled water, and ultrasonic dispersion 1min is with isopyknic 0.5mmol L
-1The cumarin aqueous solution, add H again
2O
2(ultimate density is 6.0 * 10
-8～5.0 * 10
-5Mol L
-1).At this moment, the substrate cumarin can be generated hyperfluorescence material umbelliferone by catalytic oxidation, and the maximum emission wavelength of its fluorescence-causing substance is 456nm.Behind reaction 10min, it is centrifugal to get the 2mL reactant liquor rapidly, and the supernatant after centrifugal is taken out, and on sepectrophotofluorometer, measures the fluorescence intensity of supernatant at the 456nm place.This BiFeO
3The changing value and the H of class catalase catalyst system and catalyzing fluorescence intensity at the 456nm place
2O
2Concentration is 6.0 * 10
-8～5.0 * 10
-5Mol L
-1Scope internal memory linear relationship.Utilize BiFeO of the present invention
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2Excellent properties, can be advantageously used in low concentration H in the actual samples such as food, environment and biology
2O
2The check and analysis of content.
Embodiment 3: use BiFeO
3Class catalase catalyst activation H
2O
2Come oxidation 3,3,5, the 5-tetramethyl benzidine is used for indirect determination H
2O
2Content
With the 5mg BiFeO that makes
3Pulverulent solids joins in the 5mL distilled water, and ultrasonic dispersion 1min is with isopyknic 1mmol L
-13,3,5,5-tetramethyl benzidine aqueous solution adds H again
2O
2(ultimate density is 5.0 * 10
-7～2.0 * 10
-4Mol L
-1).At this moment, substrate 3,3,5, the 5-tetramethyl benzidine can be generated blue product by catalytic oxidation, and the maximum absorption wavelength of its blue product is 650nm.Behind reaction 15min, it is centrifugal to get the 2mL reactant liquor rapidly, and the supernatant after centrifugal is taken out, and on ultraviolet-visible spectrophotometer, measures the absorbance of supernatant at the 650nm place.This BiFeO
3The changing value and the H of class catalase catalyst system and catalyzing absorbance at the 650nm place
2O
2Concentration is 5.0 * 10
-7～2.0 * 10
-4Mol L
-1Scope internal memory linear relationship.Utilize BiFeO of the present invention
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2Excellent properties, can be advantageously used in low concentration H in the actual samples such as food, environment and biology
2O
2The check and analysis of content.
Embodiment 4: use BiFeO of the present invention
3Class catalase catalyst activation H
2O
2Come the oxidation o-phenylenediamine to be used for indirect determination H
2O
2Content
With the 5mg BiFeO that makes
3Pulverulent solids joins in the 5mL distilled water, and ultrasonic dispersion 1min is with isopyknic 2.0mmol L
-1The o-phenylenediamine aqueous solution, add H again
2O
2(ultimate density is 8.0 * 10
-7～4.0 * 10
-4Mol L
-1).At this moment, the substrate o-phenylenediamine can be generated orange product by catalytic oxidation, and the maximum absorption wavelength of its orange product is 410nm.Behind reaction 15min, it is centrifugal to get the 2mL reactant liquor rapidly, and the supernatant after centrifugal is taken out, and on ultraviolet-visible spectrophotometer, measures the absorbance of supernatant at the 410nm place.This BiFeO
3The changing value and the H of class catalase catalyst system and catalyzing absorbance at the 410nm place
2O
2Concentration is 8.0 * 10
-7～4.0 * 10
-4Mol L
-1Scope internal memory linear relationship.Utilize BiFeO of the present invention
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2Excellent properties, can be advantageously used in low concentration H in the actual samples such as food, environment and biology
2O
2The check and analysis of content.
Embodiment 5: use BiFeO of the present invention
3Class catalase catalyst activation H
2O
2Come the oxidative degradation rhodamine
In 50mL concentration is 20 μ mol L
-1The rhodamine aqueous solution in add 25mg BiFeO
3Pulverulent solids, ultrasonic dispersion 1min, dark condition stir 30min down and reach the adsorption/desorption balance.Adding 0.5mL concentration then is 1.0mol L
-1H
2O
2Solution, it is centrifugal that every separated 15min gets the 2mL reactant liquor, and the supernatant after centrifugal is taken out, and on ultraviolet-visible spectrophotometer, measures the absorbance (see figure 5) of its supernatant at the 554nm place.Find that this type of hydrogen peroxide enzyme catalyst fades the whole oxidations of organic dyestuff rhodamine within 1.5h, and the further mineralising of ability, behind reaction 2h, its total organic carbon (TOC) has descended 90%.The BiFeO that utilizes the present invention to make
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2Excellent properties, can be advantageously used in the degraded and the mineralising of organic pollution in the environment.
Embodiment 6: use BiFeO of the present invention
3Class catalase catalyst activation H
2O
2Come oxidative degradation DDT
In 50mL concentration is 100 μ mol L
-1The DDT aqueous solution in add 25mg BiFeO
3Pulverulent solids, ultrasonic dispersion 1min, dark condition stir 30min down and reach the adsorption/desorption balance.Adding 1.0mL concentration then is 1.0mol L
-1H
2O
2Solution, it is centrifugal that every separated 20min gets the 2mL reactant liquor, and the supernatant after centrifugal is taken out, and under HPLC, measures its supernatant concentration.Find that this type of hydrogen peroxide enzyme catalyst effectively removes DDT within 5h, and further mineralising, behind reaction 8h, its total organic carbon (TOC) has descended 73%.The BiFeO that utilizes the present invention to make
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2Excellent properties, can be advantageously used in the degraded and the mineralising of organic pollution in the environment.
Embodiment 7: use BiFeO
3Class catalase catalyst activation H
2O
2Come the oxidative degradation bisphenol-A
In 50mL concentration is 1.0mmol L
-1The bisphenol-A aqueous solution in add 50mg BiFeO
3Pulverulent solids, ultrasonic dispersion 1min, dark condition stir 30min down and reach the adsorption/desorption balance.Adding 1.0mL concentration then is 2.0mol L
-1H
2O
2Solution, it is centrifugal that every separated 20min gets the 2mL reactant liquor, and the supernatant after centrifugal is taken out, and under HPLC, measures its supernatant concentration.Find that this type of hydrogen peroxide enzyme catalyst effectively removes bisphenol-A within 3h, and further mineralising, behind reaction 6h, its total organic carbon amount (TOC) has descended 76%.The BiFeO that utilizes the present invention to make
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2Excellent properties, can be advantageously used in the degraded and the mineralising of organic pollution in the environment.
Application examples 8: use BiFeO
3Class catalase catalyst activation H
2O
2Come the oxidative degradation tetracycline
In 50mL concentration is 0.8mmol L
-1The tetracycline aqueous solution in add 50mg BiFeO
3Pulverulent solids, ultrasonic dispersion 2min, dark condition stir 30min down and reach the adsorption/desorption balance.Adding 1.0mL concentration then is 2.0mol L
-1H
2O
2Solution, it is centrifugal that every separated 30min gets the 2mL reactant liquor, and the supernatant after centrifugal is taken out, and under HPLC, measures its supernatant concentration.Find that this type of hydrogen peroxide enzyme catalyst effectively removes tetracycline within 4h, and further mineralising, behind reaction 6h, its total organic carbon (TOC) has descended 70%.The BiFeO that utilizes the present invention to make
3Class hydrogen peroxide enzyme catalyst is activation H effectively
2O
2Excellent properties, can be advantageously used in the degraded and the mineralising of organic pollution in the environment.
Claims (1)
- The ferrous acid bismuth as the class catalase at activation H 2O 2Hydroxy radical oxidizing ferment substrate to produce strong oxidizing property is realized H in food, biology or the environmental sample 2O 2Application during content detection is analyzed, described zymolyte is benzoic acid, cumarin, P-hydroxybenzoic acid, aniline, o-phenylenediamine or 3,3,5, the 5-tetramethyl benzidine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910272236XA CN102029163B (en) | 2009-09-27 | 2009-09-27 | Catalase catalyst analog as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910272236XA CN102029163B (en) | 2009-09-27 | 2009-09-27 | Catalase catalyst analog as well as preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN108760705A (en) * | 2018-06-05 | 2018-11-06 | 华侨大学 | The fluorescence analysis method of benzoyl peroxide in a kind of detection flour |
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| CN111189823B (en) * | 2020-02-25 | 2022-02-25 | 陕西师范大学 | TMB color development system and application thereof in detection of reducing substances |
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